不同硒源对猪肉品质的影响

试验研究了酵母硒和亚硒酸钠对肥育猪肉质的影响。选用72头(61.32±1.14)kg的杜长大肥育猪,随机分成3个处理,每个处理4个重复(栏),每个栏6头猪。3个处理分别饲喂基础日粮+0.30mg/kg亚硒酸钠、基础日粮+0.30mg/kg酵母硒Ⅰ、基础日粮+0.30mg/kg酵母硒Ⅱ。试验正试期6周。试验结束后从每栏选择体重基本一致的试验猪各1头,前腔静脉采血后屠宰取背最长肌,测定肌肉pH值、滴水损失、肉色、粗蛋白、粗脂肪和水分。结果表明:与亚硒酸钠比,饲粮中添加酵母硒Ⅰ、Ⅱ降低了肌肉中滴水损失62.86%和16.59%,且酵母硒Ⅱ比酵母硒Ⅰ降低了39.68%;Huntera值分别提高了13.70%和14.42%,但均未达到显著水平(P>0.05)。     

不同硒源和硒水平对鸡抗氧化能力的影响

为了探讨不同硒源和硒水平对良凤花鸡的影响。选用340只1日龄良凤花雏鸡,试验采用2种硒源、3个添加水平按2因素完全随机法分组,每个处理组4个重复。两种硒源分别为亚硒酸钠(Na2SeO)3、蛋氨酸硒(Se-Met)。硒水平为基础日粮、基础日粮+0.15 mg/kg水平的硒、基础日粮+0.30 mg/kg水平的硒。在31日龄和56日龄时,每处理选体重相近的肉鸡6羽,共30羽采血测定血清抗氧化指标。结果表明:两种硒源对各种抗氧化指标的影响差异不显著。试验前期,添加硒水平组的GSH-Px和SOD的活力均极显著高于对照组(P<0.01),T-AOC显著高于对照组(P<0.05),MDA低于对照组,但差异不显著(P>0.05);试验后期,低硒水平组血清GSH-Px酶活性显著高于高硒水平组(P<0.05);对照组的MDA显著高于高硒水平组(P<0.05),T-AOC显著低于高硒水平组(P<0.05)。在日粮中添加0.15 mg/kg的蛋氨酸硒或亚硒酸钠能有效提高肉鸡的抗氧化能力。     

不同硒源及水平对肉鸡组织硒含量及抗氧化功能的影响

本文旨在研究和比较不同硒源及水平对爱拔益加(AA)肉鸡生长性能、组织硒含量和抗氧化功能的影响,并探讨在生产中应用较低水平(0.20 mg/kg)有机硒替代较高水平(0.30 mg/kg)无机硒的可行性.选用1日龄AA肉仔鸡600只,随机分为5组,分别饲喂基础饲粮(对照组)及在基础饲粮中添加0.30 mg/kg硒(以亚硒酸钠形式)(SS组)、0.20 mg/kg硒(以酵母硒形式)(SY Ⅰ组)、0.30 mg/kg硒(以酵母硒形式)(SYⅡ组)和0.30 mg/kg混合硒(亚硒酸钠和酵母硒形式硒各0.15mg/kg)(MS组)的试验饲粮,每组6个重复,每个重复20只鸡.试验期42 d.结果表明:1)SYⅡ组肉鸡前期平均日采食量显著低于对照组(P＜0.05),饲粮添加各水平有机硒均显著降低了肉鸡全期平均日采食量(P ＜0.05或P＜0.01).2)与对照组和SS组相比,添加各水平有机硒均显著提高了肉鸡胸肌中的硒含量(P＜0.01),且以SYⅡ组最高,显著高于其他各组(P＜0.01);SY Ⅰ组和SYⅡ组肉鸡血浆中的硒含量显著高于对照组(P ＜0.05或P＜0.01);添加各水平有机硒均显著提高了42 d肉鸡肝脏中的硒含量(P ＜0.05或P＜0.01).3)在试验前期(1 ～21 d),较SS组,SYⅡ组肉鸡血浆谷胱甘肽过氧化物酶(GSH-Px)和总超氧化物歧化酶(T-SOD)的活性均显著提高(P＜0.05);较对照组,SS组、SY Ⅰ组和SYⅡ组肉鸡肝脏T-SOD的活性均显著提高(P＜0.05或P＜0.01).在试验后期(22 ～42 d),较对照组,SS组、SY Ⅰ组和SYⅡ组肉鸡血浆和肝脏GSH-Px的活性均显著提高(P＜0.05或P＜0.01),各添加有机硒组肉鸡的血浆丙二醛含量均显著降低(P＜0.05或P＜0.01);与对照组相比,MS组肉鸡肝脏过氧化氢酶(P＜0.05)和GSH-Px的活性均显著提高(P＜0.01).由此可知,饲粮添加0.30 mg/kg酵母硒能够提高肉鸡组织硒沉积量,对其生长性能和抗氧化功能的改善效果最佳;饲粮添加0.20 mg/kg的酵母硒替代0.30 mg/kg亚硒酸钠,在改善肉鸡的生长性能、提高组织硒含量和抗氧化功能方面是可行的.     

饲粮中添加不同硒源对仔猪血浆硒含量和血清抗氧化能力的影响

试验旨在研究饲粮中添加不同硒源及不同硒水平对仔猪血浆硒含量和血清抗氧化能力的影响。选用体重相近[(12.38±0.43)kg]、健康良好的杜长大三元杂交仔猪168头,随机分成7组,每组3个重复,每个重复8头仔猪。第7组为对照组,饲喂基础饲粮;其余6组均为试验组,即分别以0.30、0.50、0.80 mg/kg 3个硒水平添加到基础饲粮中,并配成6种试验饲粮,试验期40 d。试验结果显示:不同硒源及添加水平间,仔猪血浆硒含量均高于对照组,但纳米硒在0.30、0.50 mg/kg水平时,血浆硒含量均显著高于对照组和亚硒酸钠组(P<0.05);纳米硒在0.50 mg/kg水平时,血清GSH-Px活性、T-AOC显著高于对照组(P<0.05)。同一硒源组添加不同硒水平对仔猪血清中SOD活性、MDA含量差异均不显著(P>0.05),但纳米硒组不同硒水平,仔猪血清SOD活性显著高于对照组(P<0.05),血清MDA含量低于对照组和亚硒酸钠组(P<0.05)。试验说明饲粮中添加纳米硒能显著提高仔猪血清抗氧化能力,并有提高血浆硒含量的趋势。     

不同硒源对育肥猪生长性能、血清抗氧化指标和硒沉积的影响

本研究以酵母硒为原料制备了3种工艺的硒产品（颗粒硒、粉体硒和水解硒）,并对比研究亚硒酸钠与颗粒硒、粉体硒和水解硒对育肥猪生长性能、血清抗氧化指标及硒在组织中沉积的影响。结果表明：颗粒硒、粉体硒和水解硒组的粗蛋白质、总硒含量无明显差异,但水解硒组的酸溶蛋白、氨基酸态氮和溶解率明显提高;从生长性能指标来看,不同硒源对育肥猪的平均日增重（ADG）、平均日采食量（ADFI）和料重比（F/G）无显著影响（P> 0.05）;从抗氧化指标来看,相比亚硒酸钠组,水解硒组和粉体硒组谷胱甘肽过氧化物酶（GSH-Px）的含量显著提高（P <0.05）;从血清、肝脏、和肌肉中硒含量来看,相比亚硒酸钠组,颗粒硒组、粉体硒组和水解硒组血清中硒的含量显著提高（P<0.05）,并且粉体硒组和水解硒组的肝脏和肌肉总硒含量显著高于亚硒酸钠组（P <0.05）。综上所述,水解酵母硒能提高硒酵母的酸溶蛋白含量,同时提高育肥猪的抗氧化性能及硒在组织中的快速沉积。     

不同硒源对生长肥育猪生产性能的影响

通过在生长肥育猪日粮中添加不同的硒源，比较其对生长肥育猪生长性能、肉质以及抗氧化指标的影响。结果表明：与对照组相比，试验组生长肥育猪的ADFI和ADF均有增加，F／G降低；肌肉样品中的pH值、滴水损失、肉色以及肌肉中粗蛋白、粗脂肪、水分含量均有改善。添加酵母硒在一定程度上提高血清和肌肉组织的抗氧化能力，降低了血清和肌肉中丙二醛的含量。     

不同硒源对蛋鸡组织硒含量及其抗氧化能力的影响

本试验比较研究了富硒益生菌(Selenium-enriched probiotics,SP)和亚硒酸钠(Sodium selenite,SS)对蛋鸡肝脏、肾脏和心肌硒含量及其抗氧化能力的影响。选取800羽健康罗曼蛋鸡,随机均分为8组,将2硒源分别以3个硒水平(0.2、0.5和1.0mg·kg-1)添加到基础日粮中组成6种试验日粮,同时将益生菌添加到基础日粮中作为益生菌对照,基础日粮作为空白对照,进行为期28d的饲养试验。在试验结束时,杀鸡取肝脏、肾脏和心肌分别进行硒含量及其谷胱甘肽过氧化物酶(GSH-Px)活性、过氧化氢酶(CAT)活性、超氧化物歧化酶(SOD)活性和总抗氧化能力(T-AOC)的测定。结果表明,补硒能提高蛋鸡肝脏、肾脏、心肌的硒含量以及GSH-Px、CAT、SOD的活性和T-AOC(P0.05);且随着硒添加水平的升高,肝脏、肾脏和心肌的硒含量、GSH-Px活性均有显著升高(P0.05);添加SP在提高肝脏和心肌硒含量的能力上和在提高肝脏、肾脏和心肌的GSH-Px活性、CAT活性以及肾脏的SOD活性和T-AOC的能力上要优于或显著优于SS。结果提示,补充硒能提高蛋鸡组织硒含量及其抗氧化能力,特别提倡添加低中水平(0.2和0.5mg·kg-1)SP源硒。     

不同硒源对肥育猪生产性能及肉品质的影响

通过在生长肥育猪日粮中添加不同硒源,比较不同硒源对生长肥育猪生长性能、肉质及抗氧化指标的影响。试验采用完全随机设计,选择体质量为(60±2)kg、遗传背景相同和体况良好的48头杜×长×大肥育猪,分成2个处理,每处理4个重复,每重复6头猪。试验开始前预饲1周,试验期60d。对照组以S-Se(亚硒酸钠来源的硒)0.3mg/kg和试验组Y-Se(酵母硒来源的硒)0.3mg/kg。通过观察各组采食量、平均日增质量和料重比;肌肉样品中的pH、滴水损失、肉色及肌肉中粗蛋白、粗脂肪和水分含量;肌肉和血液中的谷胱甘肽过氧化物酶和总超氧化物歧化酶的活性、总抗氧化能力及丙二醛含量等指标,探索不同硒源对肥育猪的影响。试验结果表明:与对照组相比,试验组生长肥育猪的平均日采食量和酸性洗涤纤维在数值上均有增加,料重比降低;肌肉样品中的pH、滴水损失及肉色肌肉中粗蛋白、粗脂肪和水分含量均有改善。Y-Se0.3酵母硒在一定程度上提高血清和肌肉组织的抗氧化能力,降低了血清和肌肉中丙二醛的含量。     

不同硒源对生长育肥猪生产性能的影响

近年来,研究已表明有机硒比无机硒更容易在动物体内贮存.硒酵母作为有机硒的一种,具有酵母和有机硒的双重作用.笔者拟用硒酵母和亚硒酸钠作比较,通过对生长育肥猪生长性能、肉质以及抗氧化指标的检测,验证硒酵母对生长育肥猪的生长和肉质有无影响,同时,进一步探讨硒酵母的作用机理.     

不同硒源及硒水平对大鼠生长性能、血清抗氧化能力和组织硒沉积的影响

本试验旨在考察不同硒源及硒水平对大鼠生长性能、血清抗氧化能力及组织硒沉积的影响,并以亚硒酸钠和商品酵母硒(酵母硒B)为参比,对本课题组用糖蜜和尿素为发酵底物研制的酵母硒(酵母硒A)生物学效价进行评定.将硒耗竭2周后的70只8周龄左右Wistar雌性大鼠称重,随机分到10个处理,每个处理7个重复,每个重复1只大鼠,分别饲喂不同处理的饲粮21d.处理1不添加任何形式的硒源,处理2～9在基础饲粮中分别以亚硒酸钠或酵母硒A的形式添加0.1、0.2、0.3和0.4mg/kg硒,处理10以酵母硒B形式添加0.3mg/kg硒.结果表明:1)不同硒源及硒水平对大鼠生长性能、血清谷胱甘肽过氧化物酶(GSH-Px)和超氧化物歧化酶活性影响不显著(P＞0.05);2)组织硒含量随饲粮硒水平的升高极显著增加(P＜0.01),添加酵母硒A组大鼠组织硒含量显著高于亚硒酸钠组(P＜0.05);3)以亚硒酸钠为参比,血清GSH-Px活性及肝脏、肾脏和肌肉硒含量作为判定指标,酵母硒A的相对生物学效价分别为95.9％、127.5％、114.5％和101.2％;4)添加酵母硒A和酵母硒B对大鼠生长性能、血清抗氧化能力的影响不显著(P＞0.05),但添加酵母硒A组大鼠肾脏硒含量极显著高于酵母硒B组(P＜0.01),而酵母硒B在肌肉中有更多的硒沉积(P＜0.01).结果提示:1)硒源对大鼠组织硒沉积有较大影响,酵母硒相对于亚硒酸钠具有更高的生物学效价,但不同酵母硒之间存在一定的差异;2)饲粮硒水平对大鼠血清抗氧化能力影响较小,以血清GSH-Px活性为衡量标准,0.1mg/kg硒的酵母硒和亚硒酸钠都能满足大鼠的需要,但更高水平的酵母硒可以使大鼠组织中有更多硒的储备.     

蛋鸡日粮中添加富硒酵母对鸡蛋中硒含量及分布的影响

试验选用产蛋率约为80%的罗曼褐蛋鸡35只,随机分为7组,进行为期30d的饲养试验。A组为对照组,饲以基础日粮,其余6组为试验组,B、C、D组在基础蛋鸡日粮中分别添加0.2mg/kg、0.5mg/kg、1mg/kg硒的亚硒酸钠,E、F、G组在基础蛋鸡日粮中分别添加0.2mg/kg、0.5mg/kg、1mg/kg硒的富硒酵母。试验过程中记录蛋鸡的生产性能,每隔7d从各试验组鸡所产的鸡蛋中任取3枚,测定鸡蛋中硒的含量,并于试验的第14、15天,28、29天从A、C、F组每组各取5枚鸡蛋分别测定蛋黄和蛋清的硒含量。结果显示,日粮中添加硒对蛋鸡生产性能无显著影响,能极显著提高鸡蛋中硒含量,并且硒主要存在蛋黄中,添加富硒酵母组蛋硒含量较亚硒酸钠组提高更为显著。     

Effect of different selenium sources on production performance and biochemical parameters of broilers

Selenium (Se) is an essential trace element for animal and human. Supplementation of Se usually in livestock diet has been proved as effective element. This study was conducted to investigate the effect of different sources of selenium on growth performance, slaughter performance, immune trait, oxidation resistance, meat quality and selenium content in tissue of broilers to comprehensively evaluate the effect of selenium. A total of 324 1‐day‐old AA broilers were selected and randomly allocated to three treatments of six replicates with 18 broilers each. The trial period was 42 days and was divided into two periods. Our results showed that effect of different sources of selenium on growth performance, slaughter performance, the immune status, drip loss and flesh had not significant difference (p > 0.05); while the activities of serum glutathione peroxidase (GSH‐Px), total superoxide dismutase (T‐SOD), the abilities to inhibit hydroxyl radical (OH˙) and total antioxidant capacity (T‐AOC) were significantly higher in selenium yeast than sodium selenite groups, and the contents of MDA of selenium yeast groups were significantly lower than that of sodium selenite. This study demonstrated that the different sources of selenium had no obvious effect on production performance of broilers, but significantly influenced the broiler oxidation resistance.     

不同硒源对家兔消化性能和血液生化指标的影响

本试验旨在探讨日粮不同硒源对新西兰肉兔消化性能和血液生化指标的影响。选用35日龄的断奶仔兔60只,公母各半,随机分为3组,每组5个重复,每个重复4只。在基础日粮中分别添加相同水平(0.15 mg/kg)的硒代蛋氨酸与亚硒酸钠,预试期7 d,正试期35 d。结果表明,与对照组相比,硒能显著提高粗蛋白质和粗纤维表观消化率(P<0.05),硒代蛋氨酸组显著高于亚硒酸钠组(P<0.05);硒对血清总蛋白(TP)水平有显著提高,但不同硒源之间差异不显著(P>0.05);硒能显著降低血清尿酸(UA)水平,且硒代蛋氨酸组显著低于亚硒酸钠组(P<0.05);对照组血糖(GLU)水平显著低于加硒组,但不同硒源之间无显著差异(P>0.05)。综合本试验结果,生长兔饲料中添加硒对其消化性能有增效作用,且有机硒的作用明显大于无机硒。     

不同硒源对蛋鸡生产性能和硒沉积的影响

文章旨在研究日粮添加不同硒源及不同水平对蛋鸡生产性能和鸡蛋中硒沉积量的影响。试验选择产蛋率一致的海兰褐壳蛋鸡720只,随机分为5组,每组6个重复,每个重复24只鸡。试验共设计5种日粮,对照组饲喂基础日粮(不添加硒),处理组分别在基础日粮中添加2个水平的无机硒(0.4和0.8mg/kg硒,亚硒酸钠)以及2个水平的酵母硒(0.4和0.8mg/kg硒),试验共进行16周。结果发现,无论日粮添加酵母硒还是无机硒,随着硒添加水平的升高,蛋鸡生产性能均无显著影响(P>0.05)。从第9周到试验结束,0.4mg/kg无机硒和0.8mg/kg酵母硒组较对照组和0.8mg/kg无机硒组显著提高了蛋鸡的产蛋率(P<0.05)。试验第9周开始,对照组、0.8mg/kg无机硒组和0.4mg/kg酵母硒组产蛋率显著降低(P<0.05)。随着日粮无机硒或酵母硒添加水平的升高,鸡蛋中硒的沉积量显著线性增加(P<0.05)。从第4周开始到试验结束,无机硒或酵母硒组较对照组均显著提高了鸡蛋中硒含量(P<0.05)。在同一硒添加水平及同一时间下,酵母硒较无机硒显著提高了鸡蛋硒含量(P<0.05),随着试验时间的增加,对照组鸡蛋硒含量显著降低(P<0.05)。因此,在本试验条件下,日粮硒的来源和添加水平直接影响鸡蛋硒沉积量,酵母硒较无机硒显著提高了鸡蛋硒沉积量。日粮中酵母硒的添加量可以达到0.8mg/kg,对蛋鸡生产性能无负面影响。     

Milk selenium content and speciation in response to supranutritional selenium yeast supplementation in cows

The effects of selenium (Se) yeast supplementation on performance, blood biochemical and antioxidant parameters, and milk Se content and speciation were evaluated. Thirty-six mid-lactation Holstein dairy cows were randomly assigned to 1 of 3 treatments: 1) control (basal diet containing Se at 0.11 mg/kg DM), 2) basal diet + 0.5 mg supplemental Se/kg DM (SY-0.5), and 3) basal diet + 5 mg supplemental Se/kg DM (SY-5). Selenium was supplemented as Se yeast. The trial consisted of a 1-week pretrial period and an 8-week experimental period. Milk somatic cell score decreased with SY-5 supplementation (P < 0.05), but other performance parameters were not affected (P > 0.05). The serum Se concentration increased with the increasing levels of Se yeast supplementation (P < 0.05), however, blood biochemical parameters showed few treatment effects. The antioxidant capacity of dairy cows was improved with Se yeast supplementation reflected in increased serum glutathione peroxidase activity (P < 0.05) and total antioxidant capacity (P = 0.08), and decreased malondialdehyde concentration (P < 0.05). Milk total Se concentration increased with Se dose (P < 0.05). Also, the selenomethionine concentration increased with Se dose from 13.0 ± 0.7 μg/kg in control to 33.1 ± 2.1 μg/kg in SY-0.5 and 530.4 ± 17.5 μg/kg in SY-5 cows (P < 0.05). Similarly, selenocystine concentration increased from 15.6 ± 0.9 μg/kg in control and 18.9 ± 1.1 μg/kg in SY-0.5 to 22.2 ± 1.5 μg/kg in SY-5 cows (P < 0.05). In conclusion, Se yeast is a good organic Se source to produce Se-enriched cow milk with increased Se species including selenomethionine and selenocystine. The results can provide useful information on milk Se species when a high dose Se yeast was supplemented in the cow diet.     

补饲维生素D3对育肥猪血钙与肌肉钙含量的影响

本试验旨在研究宰前短期补饲维生素D3对育肥猪血钙和肌肉钙舍量的影响.将20头体重85 kg左右的杜长大三元杂交猪随机分为5组,每组4头.试验设维生素D3的补饲量(2.5×105 Iu/d或5×105IU/d)和补饲时间(5 d或10 d)两个因素.通过对血清钙水平和内中钙含量的测定采评价补饲维生素D3对育肥猪血钙和肌肉钙含量的影响.研究结果表明:补饲维生素D3能提高育肥猪的血清钙含量和肌肉钙的含量(P＜0.05),促进钙的沉积.     

不同硒生物强化方式对火龙果硒含量的影响

为探究适用于提高火龙果硒含量的含硒肥料种类和施肥方式,以“金都1号”火龙果为试验材料,增施含硒有机肥和氨基酸硒肥,氨基酸硒肥又设置喷施（3种浓度）和淋施（3种浓度）两种施用方式,研究火龙果产量、果肉和茎硒含量的变化特征。结果表明,施用含硒肥料对火龙果产量无显著影响（p>0.05）。增施含硒有机肥和淋施氨基酸硒肥均显著提高火龙果果肉、茎硒含量（p<0.05）。所有处理组中,含硒有机肥料处理组果肉硒含量最高,达到0.019 3 mg/kg,是空白对照的5.7倍,淋施氨基酸硒肥处理组果肉硒含量范围在0.006 1 ~0.007 9 mg/kg。所有喷施氨基酸硒肥处理组果肉、茎硒含量与对照无显著差异（p>0.05）。结论：土壤施硒是火龙果更为有效的硒生物强化方式;在酸性土壤中,施用碱性含硒有机肥能更好地达到硒生物强化效果。     

Effects of selenium yeast on rumen fermentation, lactation performance and feed digestibilities in lactating dairy cows

The objective was to evaluate the effects of selenium-yeast (SY) supplementation on rumen fermentation, lactation performance and feed digestibilities in dairy cows. Twenty-eight multiparous (2.5 ± 0.3 parity) Holstein dairy cows, averaging 625 ± 18.2 kg of BW, 63 ± 3.0 days in milk and average daily milk production of 26.2 ± 0.5 kg/cow were used in a replicated 4 × 4 Latin square experiment. Treatments were: control, LSY, MSY and HSY with 0, 150, 300 and 450 mg selenium yeast (Sel-Plex®, 1 g/kg selenium, produced from Saccharomyces cerevisiae CNCM I-3060) per kg of diet dry matter (DM), respectively. Experimental periods were 45 days with 30 days of adaptation and 15 days of sampling. Ruminal pH was lower for MSY than for control and was linearly (P = 0.03) decreased, whereas total VFA concentration was linearly and quadratically (P ≤ 0.01) increased with increasing SY supplementation, with the least for control, followed by LSY and HSY, and the highest for MSY. Ratio of acetate to propionate was lower for LSY, MSY and HSY than for control, and was linearly and quadratically (P ≤ 0.01) decreased as SY supplementation increased due to an increase in molar proportion of propionate. Ruminal ammonia N content was lower (P ≤ 0.01) for MSY and HSY than for control and was linearly decreased (P ≤ 0.01) with increasing SY supplementation. Dry matter intake, proportions and yields of milk fat, protein and lactose were not affected (P > 0.05). Milk yields and 4% FCM were higher (P ≤ 0.05) for LSY and MSY than for HSY and control. Milk selenium content was linearly and quadratically (P ≤ 0.01) increased as increasing SY supplementation, with the least for control, followed by LSY and MSY, and the highest for HSY. Digestibilities of DM, organic matter (OM), crude protein (CP), ether extract (EE), aNDF and ADF in the total tract were higher (P < 0.01) for LSY and MSY than for control and HSY. The results indicate that supplementation of diet with SY improved rumen fermentation, milk yields, milk selenium, and feed digestion. It was suggested that the SY stimulated the digestive microorganisms or enzymes in a dose-dependent manner. In the experimental conditions of this trial, the optimum selenium-yeast dose was about 300 mg per kg diet DM.     

不同酵母菌种富硒能力比较与发酵条件优化

将啤酒酵母、产朊假丝酵母、克鲁斯假丝酵母和葡萄汁酵母4种酵母菌株分别接种到不同硒浓度的YEPD琼脂培养基中,观察不同酵母菌株的耐硒能力;比较4株酵母菌株在相同硒浓度下的生长情况以及有机硒的转化率,选出1株富硒能力强的酵母,并对发酵条件进行优化,确定最佳的硒添加量和添加方式。结果表明:啤酒酵母的耐硒能力和富硒能力均为最强,当硒添加量为20μg/mL时,在发酵12,18和24 h分3次添加,啤酒酵母的综合富硒效果最好,活菌数为2.74×108cfu/mL,有机硒转化率为85.5%。