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1.
Molecular markers and their applications in wheat breeding 总被引:40,自引:0,他引:40
In recent years, considerable emphasis has been placed on the development of molecular markers to be used for a variety of objectives. This review attempts to give an account of different molecular markers—restriction fragment length polymorphisms (RFLPs), random amplified polymorphic DNAs (RAPDs), sequence-tagged sites (STS), DNA amplification fingerprinting (DAF), amplified fragment length polymorphisms (AFLPs) and microsatellites (STMS)—currently available for genome mapping and for tagging different traits in wheat. Other markers, including microsatellite-primed polymerase chain reaction (MP-PCR), expressed sequence tags (ESTs) and single nucleotide polymorphisms (SNPs) are also discussed. Recent information on synteny in cereal genomes, marker-assisted selection, marker validation and their relevance to cereal breeding in general and wheat breeding in particular are also examined. 相似文献
2.
Wild emmer, Triticum dicoccoides, the progenitor of cultivated wheat, harbors rich genetic resources for wheat improvement. They include many agronomic traits
such as abiotic stress tolerances (salt, drought and heat), biotic stress tolerances (powdery mildew, rusts, and Fusarium
head blight), grain protein quality and quantity, and micronutrient concentrations (Zn, Fe, and Mn). In this review, we summarize
(1) traits and controlling genes identified and mapped in T. dicoccoides; and (2) the genes transferred to cultivated wheat from T. dicoccoides. These genes, controlling important agronomic traits such as disease resistance, high protein and micronutrient content,
should contribute to wheat production and food nutrition. However, most of the rich genetic reservoir in wild emmer remains
untapped, highlighting the need for further exploration and utilization for long-term wheat breeding programs. 相似文献
3.
Significant progress has been made in the characterization of loci controlling traits of importance using molecular markers.
A number of markers are currently available in wheat for genes of interest to the breeders. Markers can be used to better
characterize parental material, thereby improving the efficiency and effectiveness of parental selection for crossing and
to track genes in segregating progenies through the selection process. Although a number of breeding programs are using molecular
markers at modest levels, the costs associated with marker assisted selection (MAS) are frequently cited as the main constraint
to their wide-spread use by plant breeders. However, this is likely to change when user-friendly, high-throughput, automated
marker technologies based on single nucleotide polymorphisms become available. These evolving technologies will increase the
number of available markers, and will improve the efficiency, throughput, and cost effectiveness of MAS, thereby making it
more attractive and affordable to many breeding programs. This article examines the extent to which molecular markers have
been used at the International Maize and Wheat Improvement Center (CIMMYT) in applied wheat breeding and reviews the limited
publicly available information on MAS from other wheat breeding programs. As markers are currently available for relatively
few traits, we believe that MAS must be integrated with ongoing conventional breeding to maximize its impact. When used in
tandem with phenotypic selection, MAS will improve response to selection for certain traits, thereby increasing rates of genetic
progress. 相似文献
4.
本研究以一个蝴蝶兰杂交组合Phal.462×Phal.20后代为材料,测定其后代群体中88个单株的叶长、叶宽和株幅,并基于已构建的AFLP分子连锁图谱,应用区间作图法对调控这3个性状的基因进行了QTL分析。统计分析结果表明,3个性状在群体中呈连续分布,具有数量性状的典型特征,叶长与株幅之间存在极显著正相关。QTL分析结果表明,在父本中共检测到QTL60个,分布于8个连锁群上,包括控制叶长性状的QTL20个,控制叶宽性状19个,控制株幅性状21个;各QTL的LOD值在3.05~14.78之间,可解释44.1%~89.4%的表型变异。在母本中共检测到QTL28个,分布于五个连锁群上,包括控制叶长性状10个,控制叶宽性状8个,控制株幅性状10个;各QTL的LOD值在3.1~9.24之间,可解释57.7%~76.3%的表型变异。这是蝴蝶兰上首个QTL分析研究,可为今后蝴蝶兰的基因克隆与分子标记辅助育种等提供基础。 相似文献
5.
A. Badea F. Eudes R. J. Graf A. Laroche D. A. Gaudet R. S. Sadasivaiah 《Euphytica》2008,164(3):803-819
Fusarium head blight (FHB) caused by Fusarium species, is among the most devastating wheat diseases, causing losses in numerous sectors of the grain industry through yield
and quality reduction, and the accumulation of poisonous mycotoxins. A germplasm collection of spring and winter wheat, including
nine reference cultivars, was tested for Type II FHB resistance and deoxynivalenol (DON) content. Genetic diversity was evaluated
on the basis of Simple Sequence Repeat (SSR) markers linked to FHB resistance quantitative trait loci (QTLs) and Diversity
Arrays Technology (DArT) markers. The allele size of the SSR markers linked to FHB resistance QTLs from known resistance sources
was compared to a germplasm collection to determine the presence of these QTLs and to identify potentially novel sources of
resistance. Forty-two accessions were identified as resistant or moderately resistant to Fusarium spread, and two also had very low DON concentrations. Genetic relationships among wheat accessions were generally consistent
with their geographic distribution and pedigree. SSR analysis revealed that several resistant accessions carried up to four
of the tested QTLs. Resistant and moderately resistant lines without any known QTLs are considered to be novel sources of
resistance that could be used for further genetic studies. 相似文献
6.
Molecular markers in Brassica oilseed breeding: current status and future possibilities 总被引:6,自引:0,他引:6
As PCR techniques have developed over the last 15 years, a wealth of new DNA marker technologies have arisen which have enabled the generation of high‐density molecular maps for all the major Brassica crop species. Molecular markers have also been heavily used in analyses of genetic diversity in Brassica crops. The majority of the work utilizing molecular markers in Brassica oilseed breeding has to date been based on genetic mapping using various DNA marker systems in segregating populations generated for specific investigations of particular traits of interest. For numerous qualitative traits, traditional mapping approaches have led to the development of marker‐assisted selection strategies in oilseed Brassica breeding, and in some cases to map‐based cloning of the responsible genes. For quantitative traits, however, it has become apparent that traditional mapping of quantitative trait loci (QTL) is often not sufficient to develop effective markers for trait introgression or for identification of the genes responsible. In this case, allele‐trait association studies in non‐structured genetic populations represent an interesting new approach, provided the degree of gametic phase disequilibrium between the QTL and the marker loci is sufficient. Because Brassica species represent the closest crop plant relatives to the model plant Arabidopsis thaliana, significant progress will be achieved in the coming years through integration of candidate gene approaches in crop brassicas, using the detailed information now available for the Arabidopsis genome. Integration of information from the model plant with the increasing supply of data from physical mapping and sequencing of the diploid Brassica genomes will undoubtedly give great insight into the genetics underlying both simple and complex traits in oilseed rape. This review describes the current use of available genetic marker technologies in oilseed rape breeding and provides an outlook for use of new technologies, including single‐nucleotide polymorphism markers, candidate gene approaches and allele‐trait association studies. 相似文献
7.
Recent advances in genomics bioinformatics now offer real opportunities for dissecting complex traits into their component sub-traits which will simplify the process of developing the tools necessary to manipulate the underlying genes 《分子植物育种》2007,5(2):199-200
Recent advances in genomics and bioinformatics now offer real opportunities for dissecting complex Waits into their component sub-traits, which will simplify the process of developing the tools necessary to manipulate the underlying genes (Varshney et al., 2005). The value of molecular markers as a complement to phenotyping under several breeding scenarios is largely unquestioned, as demonstrated by the increasing number of successful studies published (Varshney et al., 2006). However most experiments have targeted crop improvement for disease resistance, morphological waits or quality waits (Franca et al., 2005) and there is still some way to go before markers can be used routinely and ubiquitously to breed for complex waits, such as tolerance to abiotic stress (Ribaut and Ragot, 2007). The combination of genetic mapping and association studies has considerable potential to generate a catalogue of genetic variation, and thereby present novel opportunities for selection based on genome-wide scans (Biswas and Akey, 2006). Nevertheless, it remains to be seen whether the outcome of gene interactions, particularly where significant gene networks are involved, 相似文献
8.
Characterization of dinucleotide and trinucleotide EST-derived microsatellites in the wheat genome 总被引:6,自引:0,他引:6
Over the past decade microsatellites or simple sequence repeats (SSRs) have attracted a considerable amount of attention from
researchers. The aim of the present paper was to analyse expressed sequence tag-derived SSR (EST-SSR) marker variability in
wheat and to investigate the relationships between the number and type of repeat units and the level of microsatellite polymorphism.
Two hundred and forty-one new EST-SSR markers available in a public database () were characterized in eight durum wheat cultivars (Svevo, Ciccio, Primadur, Duilio, Meridiano, Claudio, Latino, Messapia),
two accessions of Triticum turgidum var. dicoccoides (MG4343, MG29896), one accession of T. turgidum var. dicoccum (MG5323) and in the common wheat cv. Chinese Spring. Of these, 201 primer pairs (83.4%) amplified PCR products successfully,
while the remaining 40 (16.6%) failed to amplify any product. Of the EST-SSRs analysed, 45.2% of the primer pairs amplified
one or two PCR products. Multiple discrete PCR products were observed among both di- and trinucleotide EST-SSR markers (31.2
and 40.5%, respectively). Markers based on dinucleotide microsatellites were more polymorphic than those based on trinucleotide
SSRs in the 12 wheat genotypes tested (68.9 and 52.7%, respectively). An average of 2.5 alleles for dinucleotide and 2.0 alleles
for trinucleotide SSRs was observed. The data reported in the present work indicate the presence of a significant relationship
between motif sequence types and polymorphism. The primer set based on the AG repeat motif showed the lowest percentage of
polymorphism (55.0%), while the primer set based on the AC repeat motif showed t he highest percentage (85.0%). Among trinucleotide
SSRs, the AGG microsatellite markers showed the highest percentage of polymorphism (70.0%), and the ACG motif the lowest value
(25.0%). The characterization of these new EST-SSR markers and the results of our studyon the effect of repeat number and
type of motifs could have important applications in the genetic analysis of agronomically important traits, quantitative trait
locus discovery and marker-assisted selection. 相似文献
9.
Kun-Pu Zhang Guang-Feng Chen Liang Zhao Bin Liu Xian-Bin Xu Ji-Chun Tian 《Euphytica》2009,165(3):471-484
Flour color is an important trait in the assessment of flour quality for the production of many end products. In this study,
quantitative trait loci (QTLs) with additive effects, epistatic effects, and QTL × environment (QE) interactions for flour
color in bread wheat (Triticum aestivum L.) were studied, using a set of 168 doubled haploid (DH) lines derived from a Huapei 3 × Yumai 57 cross. A genetic map was
constructed using 283 simple sequence repeats (SSR) and 22 expressed sequence tags (EST)-SSR markers. The DH and parents were
evaluated for flour color in three environments. QTL analyses were performed using QTLNetwork 2.0 software based on a mixed
linear model approach. A total of 18 additive QTLs and 24 pairs of epistatic QTLs were detected for flour color, which were
distributed on 19 of the 21 chromosomes. One major QTL, qa1B, closely linked to barc372 0.1 cM, could account for 25.64% of the phenotypic variation of a* without any influence from the environments. So qa1B could be used in the molecular marker-assisted selection (MAS) in wheat breeding programs. The results showed that both additive
and epistatic effects were important genetic basis for flour color, and were also sometimes subject to environmental modifications.
The information obtained in this study should be useful for manipulating the QTLs for flour color by MAS in wheat breeding
programs.
Kun-Pu Zhang and Guang-Feng Chen contributed equally to this study. 相似文献
10.
Stine Tuvesson Christophe Dayteg Per Hagberg Outi Manninen Pirjo Tanhuanpää Teija Tenhola-Roininen Elina Kiviharju Jens Weyen Jutta Förster Joerg Schondelmaier Julia Lafferty Marion Marn Andreas Fleck 《Euphytica》2007,158(3):305-312
The breeding companies and laboratories involved in this article cover a wide range of crops grown in the temperate climate
zone: small grain cereals, oilseed crops, forage crops, turf, vegetables and potato. Speed and efficiency are becoming increasingly
important in variety breeding and doubled haploids (DH) and genetic markers are important biotechnological tools to accelerate
materials to market. Collaborative research between universities, research institutions and breeding companies has resulted
in the routine use of DH technology and molecular markers in practical breeding of barley, wheat and rapeseed. DH populations
have been established not only for barley, wheat and rapeseed, but for rye, oat and triticale, where DH technology is less
developed. A driver here is the value of the crop e.g. although wheat is less responsive to DH production the value of the
end product makes the effort worthwhile. Simple and rapid DNA extraction methods used in high-throughput marker assisted selection
(MAS) systems are essential for routine use of markers. MAS is used both to monitor the presence of genes of interest and
also to monitor the genetic background. DH technology in forage, turf and vegetables is still in progress and the practical
use of markers in all crops is limited by access to trait linked markers. Collaboration and technology transfer with universities,
research institutions and breeding companies is essential for the improvement of both DH protocols in recalcitrant crops and
marker technology in all crops. 相似文献
11.
分子标记辅助选择小麦抗白粉病基因Pm2、Pm4a、Pm21 的聚合体 总被引:19,自引:0,他引:19
利用与小麦抗白粉病基因Pm2、Pm4a和Pm21紧密连锁的PCR标记,对含有Pm2、Pm4a和Pm21的小麦品系复合杂交后代经3轮分子标记选择,得到了一批聚合有Pm2+Pm4a+Pm21 3个基因的抗病植株,以及若干株Pm2+Pm21、Pm4a+Pm21和Pm2+Pm4a 2个基因聚合的植株。同时,还对中选植株进行抗病性人工接种鉴定。结果表明,含有Pm21的聚合体与Pm21基因单独存在时抗性相当,均对白粉病免疫,聚合体Pm2+Pm4a的抗性好于Pm2或Pm4a单独存在时的抗性。为降低分子标记选择成本,将检测Pm4a和Pm21的2种PCR放在一个反应体系中进行,扩增产物经1次电泳,可同时检测出Pm4a和Pm21,不同引物之间没有明显交叉扩增现象。 相似文献
12.
Mandy Christopher Emma Mace David Jordan David Rodgers Paul McGowan Ian Delacy Phillip Banks John Sheppard David Butler David Poulsen 《Euphytica》2007,154(3):307-316
The aim of the pedigree-based genome mapping project is to investigate and develop systems for implementing marker assisted
selection to improve the efficiency of selection and increase the rate of genetic gain in breeding programs. Pedigree-based
whole genome marker application provides a vehicle for incorporating marker technologies into applied breeding programs by
bridging the gap between marker–trait association and marker implementation. We report on the development of protocols for
implementation of pedigree-based whole genome marker analysis in breeding programs within the Australian northern winter cereals
region. Examples of applications from the Queensland DPI&F wheat and barley breeding programs are provided, commenting on
the use of microsatellites and other types of molecular markers for routine genomic analysis, the integration of genotypic,
phenotypic and pedigree information for targeted wheat and barley lines, the genomic impacts of strong selection pressure
in case study pedigrees, and directions for future pedigree-based marker development and analysis. 相似文献
13.
Tariq Mahmood Muhammad H. Rahman Gary R. Stringam Francis Yeh Allen G. Good 《Euphytica》2007,154(1-2):101-111
A doubled haploid population of Brassica juncea, developed from a cross between two parental lines differing for days to maturity, was used to study the efficiency of indirect
selection for a primary trait through selection of secondary trait(s) over direct selection for the primary trait when quantitative
trait loci information is available for both primary and secondary traits, and applied. Days to maturity was considered as
primary trait, while days to first flowering, days to end of flowering, flowering period and plant height were considered
as secondary traits. An RFLP linkage map was employed for QTL analysis of maturity and maturity-determinant traits, and a
stable QTL B6 simultaneously affecting these two types of traits was identified. This linked QTL explained 11.7% phenotypic
variation for days to maturity, 20.7% variation for days to first flowering, 24.3% variation for days to end of flowering
and 14.4% variation for plant height. Phenotypic evaluation of maturity and/or maturity-determinant traits, viz. days to first
flowering, days to end of flowering and plant height revealed that limited genetic advance for early maturity can be achieved
through phenotypic selection of the primary and/or the secondary trait(s). However, the estimates of genetic advance for early
maturity based on combined phenotypic evaluation and linked QTL data was found to be, at least, three times higher compared
to genetic advance based on phenotypic evaluation only, demonstrating the potential of marker-assisted selection in breeding
for early maturity in B. juncea. 相似文献
14.
Mark E. Sorrells 《Euphytica》2007,157(3):299-306
There have been many changes impacting wheat improvement since the 1996 International Maize and Wheat Improvement Center Wheat
Yield Symposium. This review highlights a few of the technological advances and impacts of new knowledge on wheat improvement
that have occurred in the past 10 years as well as on-going challenges. One of the most dramatic discoveries has been the
revelation that the genomes of graminaceous crops are complex, rapidly evolving, and heterogeneous, even within species. The
use of marker-assisted selection for improving complex traits is one of the challenges facing wheat breeders. Integration
of association analysis into conventional breeding programs is proposed as a crop improvement strategy that has the potential
to improve the efficiency of molecular breeding. 相似文献
15.
An introduction to markers, quantitative trait loci (QTL) mapping and marker-assisted selection for crop improvement: The basic concepts 总被引:24,自引:2,他引:24
Recognizing the enormous potential of DNA markers in plant breeding, many agricultural research centers and plant breeding institutes have adopted the capacity for marker development and marker-assisted selection (MAS). However, due to rapid developments in marker technology, statistical methodology for identifying quantitative trait loci (QTLs) and the jargon used by molecular biologists, the utility of DNA markers in plant breeding may not be clearly understood by non-molecular biologists. This review provides an introduction to DNA markers and the concept of polymorphism, linkage analysis and map construction, the principles of QTL analysis and how markers may be applied in breeding programs using MAS. This review has been specifically written for readers who have only a basic knowledge of molecular biology and/or plant genetics. Its format is therefore ideal for conventional plant breeders, physiologists, pathologists, other plant scientists and students. 相似文献
16.
B. B. Dholakia J. S. S. Ammiraju H. Singh M. D. Lagu M. S. Röder V. S. Rao H. S. Dhaliwal P. K. Ranjekar V. S. Gupta W. E. Weber 《Plant Breeding》2003,122(5):392-395
The economic value of wheat grain is determined by the kernel morphology which is an important parameter for manufacturing different food products requiring specific grain characteristics. Although kernel size and shape have emerged as important breeding objectives, not much information is available about the number or location of associated gene(s)/quantitative trait loci. In the present study, a recombinant inbred line population of 106 plants (F7) was phenotyped for four traits, namely kernel length, width, weight and factor form density (FFD) and genotyped with different polymerase chain reaction‐based markers. Transgressive segregants were observed for all the traits and genetic correlation studies showed positive correlations between the majority of the traits. The number of markers associated with each trait ranged from two to nine and the phenotypic contribution by an individual marker ranged from 3.3 to 16.6%. Many of the markers showed linkage to more than one trait. Strategies for improving the wheat grain quality traits and the utility of such markers in marker‐assisted selection (MAS) efforts are discussed. 相似文献
17.
QTL mapping for flour and noodle colour components and yellow pigment content in common wheat 总被引:1,自引:0,他引:1
Yelun Zhang Yunpeng Wu Yonggui Xiao Zhonghu He Yong Zhang Jun Yan Yan Zhang Xianchun Xia Chuanxi Ma 《Euphytica》2009,165(3):435-444
Improvement of flour colour is an important breeding objective for various wheat-based end-products. The objectives of this
study were to identify quantitative trait loci (QTL) for flour colour components and yellow pigment content (YPC), using 240
recombinant inbred lines (RILs) derived from a cross between the Chinese wheat cultivars PH82-2 and Neixiang 188. Field trials
were performed in a Latinized α-lattice design in Anyang and Jiaozuo, Henan Province and Taian, Shandong, in the 2005–2006
and 2006–2007 cropping seasons providing data for six environments. One hundred and eighty-eight polymorphic SSR markers,
rye secalin marker Sec1, STS markers YP7A for a phytoene synthase gene (Psy-A1), and four glutenin subunit markers, were used to genotype the population and construct the linkage map for subsequent QTL
analysis. Two major QTL were detected for YPC, associated with 1RS (1B.1R translocation) and the Psy-A1 (7A) gene, explaining 31.9% and 33.9% of the phenotypic variances, respectively. 1RS also had large influences on Fa*, Fb*,
KJ, NL*and Nb*, and Psy-A1 genes showed large effects on Fa*, Fb*, Kj, Fci, NL*, Na* and Nb*, explaining from 4.5 to 26.1% and 4.3 to 35.9% of the phenotypic
variances, respectively. In addition, QTL for flour colour parameters and yellow pigment content were also detected on chromosomes
1A and 4A, accounting for 1.5–4.1% of the phenotypic variance. The genetic effect of the 1B.1R translocation on flour colour
parameters was also discussed. 相似文献
18.
Chunmei Wang Yiping Zhang Dejun Han Zhensheng Kang Guiping Li Aizhong Cao Peidu Chen 《Euphytica》2008,159(3):359-366
Stripe (yellow) rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most devastating wheat diseases worldwide. Triticum aestivum-Haynaldia villosa 6VS/6AL translocation lines carrying the Yr26 gene on chromosome 1B, are resistant to most races of Pst used in virulence tests. In order to better utilize Yr26 for wheat improvement, we attempted to screen SSR and EST-based STS markers closely linked with Yr26. A total of 500 F2 plants and the F2:3 progenies derived from a cross between 92R137 and susceptible cultivar Yangmai 5 were inoculated with race CYR32. The analysis
confirmed that stripe rust resistance was controlled by a single dominant gene, Yr26. Among 35 pairs of genomic SSR markers and 81 pairs of STS markers derived from EST sequences located on chromosome 1B, Yr26 was flanked by 5 SSR and 7 STS markers. The markers were mapped in deletion bins using CS aneuploid and deletion lines. The
closest flanking marker loci, Xwe173 and Xbarc181, mapped in 1BL and the genetic distances from Yr26 were 1.4 cM and 6.7 cM, respectively. Some of these markers were previously reported on 1BS. Eight common wheat cultivars
and lines developed from the T. aestivum-H. villosa 6VS/6AL translocation lines by different research groups were tested for presence of the markers. Five lines with Yr26 carried the flanking markers whereas three lines without Yr26 did not. The results indicated that the flanking markers should be useful in marker-assisted selection for incorporating
Yr26 into wheat cultivars. 相似文献
19.
Passoupathy Rajendrakumar Akshaya Kumar Biswal Kannabiran Sakthivel Maganti Sheshu Madhav Chirravuri Neeraja Sena M. Balachandran Kommoju Srinivasarao Podishetty Natarajkumar Yadla Hari Kalidindi Sujatha Raman M. Sundaram 《Euphytica》2009,169(2):263-271
SSR markers targeting (GATA)
n
motifs are known to be highly polymorphic and useful in many organisms. (GATA)
n
motif specific SSR markers covering the whole rice genome are not available. The present study was carried out with an objective
to identify class I rice microsatellites in the rice genome with (GATA)n motifs, in-silico, and validate their potential as molecular markers. A total of 243 such motifs were identified; 65 of these
were present in the genic region, 59 were in the upstream region and the remaining motifs were found in the intergenic regions.
Many of the (GATA)
n
motifs were found within and/or upstream of genes associated with biotic or abiotic stress tolerance. A total of 230 PCR-based
markers targeting all the class I (GATA)
n
microsatellites were developed and 35 of these markers spread across the rice genome were validated in a set of 24 representative
rice varieties belonging to five distinct cultivar groups. All the markers were polymorphic, with average polymorphism information
content (PIC) value of 0.61, and the rice cultivars could be uniquely distinguished into different cultivar groups based on
marker analysis. These informative markers targeting (GATA)
n
motifs representing a new set of markers in rice will be highly useful for genetic studies and marker-assisted selection.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users.
Passoupathy Rajendrakumar, Akshaya Kumar Biswal and Kannabiran Sakthivel contributed equally. 相似文献
20.
The potential of genomic‐assisted breeding to improve Fusarium head blight resistance in winter durum wheat
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Thomas Miedaner Henry Desaint Hermann Buerstmayr C. Friedrich H. Longin Tobias Würschum 《Plant Breeding》2017,136(5):610-619
Durum wheat is the most important tetraploid wheat mainly used for semolina and pasta production, but is notorious for its high susceptibility to Fusarium head blight (FHB). Our objectives were to identify and characterize quantitative trait loci (QTL) in winter durum and to evaluate the potential of genomic approaches for the improvement of FHB resistance. Here, we employed an international panel of 170 winter and 14 spring durum lines, phenotyped for Fusarium culmorum resistance at five environments. Heading date, plant height and mean FHB severity showed significant genotypic variation with high heritabilities and FHB resistance was negatively correlated with both heading date and plant height. The dwarfing gene Rht‐B1 significantly affected FHB resistance and the genome‐wide association scan identified eight additional QTL affecting FHB resistance, explaining between 1% and 14% of the genotypic variation. A genome‐wide prediction approach yielded only a slightly improved predictive ability compared to marker‐assisted selection based on the four strongest QTL. In conclusion, FHB resistance in durum wheat is a highly quantitative trait and in breeding programmes may best be tackled by classical high‐throughput recurrent phenotypic selection that can be assisted by genomic prediction if marker profiles are available. 相似文献