共查询到20条相似文献,搜索用时 78 毫秒
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1 材料与方法 1998~ 2 0 0 0年进行了本试验 ,选择兴山县夏橙研究所和秭归县滩乡陈家湾村分别为锦橙 10 1和奥灵达夏橙果实的采样点 ,以普通锦橙和伏令夏橙为对照品种。锦橙 10 1和普通锦橙的树龄为 14年生 ,枳砧。奥灵达夏橙和伏令夏橙的树龄 5年生 ,基砧枳 ,中间砧锦橙。每年分别在 11月下旬至 12月上旬、4月下旬采集果样 ,送华中农业大学食品生化系实验室分析可溶性固形物含量、含酸量、固酸比和出汁率等。根据国内有关柑桔果汁加工的资料 ,综合制定出甜橙非浓缩原汁优质标准指标 :果汁可溶性固形物含量≥ 10 % ,含酸量 0 6 %~… 相似文献
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从湖南柑桔主产县(市)代表性柑桔生产基地果园采集12份普通甜橙(以冰糖橙为主)果实样品,17份脐橙(以纽荷尔脐橙为主)果实样品,包括13个甜橙品种,对单果质量、果实横纵径、种子数、出汁率、可食率、可溶性固形物含量、可滴定酸含量、固酸比和维生素C含量等品质指标进行测定和比较分析。结果表明,冰糖橙、纽荷尔脐橙两个主栽品种在湖南各个产区果实品质性状稳定,湘南地区甜橙果实品质整体较好;卡拉卡拉脐橙、锦秀冰糖橙和橘湘元糖橙等品种的果实品质表现优异;早红脐橙、崀丰脐橙和园丰脐橙可以延长湖南脐橙的采收期。 相似文献
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世界柑桔汁消费变化趋势及影响因素 总被引:3,自引:0,他引:3
柑桔汁因其优美的色泽、丰富的营养、宜人的口味与芳香,是世界上最受欢迎、贸易量最大的果汁产品之一,大约占据了世界2/3的果汁市场。按制汁原料的不同可分为甜橙汁、葡萄柚汁、柠檬汁、温州蜜柑汁等多种类型,其中甜橙汁是最重要的柑桔汁,占柑桔汁总量的95%左右。全球橙汁的生产主要集中在巴西的圣保罗和美国的佛罗里达, 相似文献
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AIM:To establish a fast, accurate and economical technique for culturing mouse pulmonary arteriolar smooth muscle cells (PASMCs), and to explore the effects of hypoxia on the proliferation and apoptosis of the PASMCs. METHODS:In sterile condition, the pulmonary artery was isolated from the male BALB/c mice by digesting with collagenase I, and the cells were cultured in fetal bovine serum-coated flask. Centrifugal procedure was not used during the cell passage. The cell morphology was observed under an inverted phase-contrast microscope. α-Smooth muscle actin was identified by immunocytochemistry and immunofluorescence. The effects of hypoxia on the proliferation and apoptosis of the PASMCs were detected by CCK-8 assay and TUNEL assay. RESULTS:PASMCs were identified by the methods of immunocytochemistry, immunofluorescence staining and observation of morphology. Unlike the rat PASMCs with typical subcultured peak-vally pattern, the mouse PASMCs showed a lot different without a peak-vally pattern. The cells could be subcultured after 5 d to 7 d and there was 3 to 5 generations depending on the activity of the cells. CCK-8 assay demonstrated that the A values of PASMCs exposed to hypoxia increased after 24 h (P<0.05) as compared with normoxia. TUNEL result showed that the apoptotic index of the PASMCs in hypoxia decreased after 24 h (P<0.05). CONCLUSION:This technique for obtaining cultured mouse PASMCs is simple, fast, accurate and economical. The digestion time is easy to control. Hypoxia promotes the proliferation and inhibits the apoptosis of PASMCs. 相似文献
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小檗碱和壳聚糖抗蔬菜病原真菌活性测定及复合膜制备 总被引:2,自引:0,他引:2
研究了小檗碱和壳聚糖对几种常见蔬菜病原真菌的抑制活性,以及以小檗碱和壳聚糖为主料制备复合膜的方法,并测定了该膜的药物释放效果。试验表明:低浓度(0.234 mg/mL)小檗碱即可抑制辣椒炭疽病菌(Vermicularia capsici)等5种蔬菜病原真菌的生长。20 mg/mL浓度壳聚糖对番茄灰霉病菌(Botrytis cinerea)的抑制率高达65%,而对其余4种果蔬病原真菌也有一定的抑制作用。为了集成这2种天然化合物的优点,制备了小檗碱-壳聚糖复合膜,该膜具有缓释功能,在模拟外部环境(磷酸缓冲液,pH 6.8)条件下,20 d小檗碱累计释放率接近25%,提示其在果蔬贮藏抗菌中的应用价值。 相似文献
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钾肥在小白菜和萝卜上的施用效果 总被引:6,自引:0,他引:6
采用盆栽试验方法研究了施用钾肥对小白菜、萝卜生长发育、产量、品质及钾素含量的影响。结果表明 ,施用钾肥对小白菜和水萝卜的株高、叶片数无显著的影响 ,但可提高水萝卜块根的产量 ,提高幅度为 31.0 4 %~ 97.93%。施用钾肥可提高小白菜和萝卜的蛋白质和VC含量 ,降低硝酸盐含量 ,增加植株的钾素含量 相似文献
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Fresh onion seeds desiccated to 6.0% seed moisture content (SMC) were stored in various packaging materials under different storage conditions. Seeds packed in aluminum-laminated pouches beside those stored with silica gel at 25 °C maintained satisfactory germinability and vigour after 12 months. Desiccated seeds stored in moisture impervious containers produced more vigourous seedlings. Germination potential of onion seeds increased with reduced SMC besides storage in moisture impervious packets along with desiccants as physiological and biochemical attributes are regulated. Seed viability and vigour decreased with accelerated ageing due to increased lipid peroxidation, decreased activities of several free radical and peroxide scavenging enzymes. Electrical conductance of seed leachates also increased with ageing. Thus, adoption of appropriate storage temperature and moisture control techniques would significantly affect onion seed quality, which was due to minimum accumulation of free peroxide radicals and enhanced activity of free radical scavenging enzymes. 相似文献
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以‘小樱桃’文心兰为材料克隆了铁氧还蛋白氧化还原酶(FNR)两种类型基因RFNR(Root-type Ferredoxin-NADP+ oxidoreductase)和LFNR(Leaf-type Ferredoxin-NADP+ oxidoreductase)。生物信息学分析表明,RFNR与LFNR都属于FNR-like超家族,具有黄素腺嘌呤二核苷酸(flavin adenine dinucleotide,FAD)和烟酰胺腺嘌呤二核苷酸(nicotinamide adenine dinucleotide,NAD)功能结合域,在进化过程中比较保守;两种类型的FNR在氨基酸组成、蛋白结构和构型方面存在明显差异,RFNR比LFNR有更强的保守性。FNR蛋白亚细胞定位结果表明文心兰FNR蛋白都定位于叶绿体。实时荧光定量PCR分析表明,LFNR和RFNR具有器官表达特异性:LFNR更多地在叶中表达,RFNR更多地在根中表达;在软腐病胁迫下LFNR下调响应,RFNR上调响应;两种类型的FNR在盐胁迫以及高温胁迫处理时上调响应,但RFNR响应较快且强度更大;LFNR和RFNR在水杨酸(salicylic acid,SA)处理时轻微波动,茉莉酸甲酯(methyl jasmonate,MeJA)处理时呈现单峰反应。文心兰两种类型的FNR基因在不同类型的胁迫中显示相似的和有区别的表达特性,表明其具有不同的胁迫响应分子机理。 相似文献
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The effects of combinations of salinity (no salt, 2000 p.p.m. or 4000 p.p.m. of CaCl2 and NaCl, 1:1) and water table (30 cm, 60 cm or 90 cm from the soil surface), on the vegetative growth and tolerance of ‘Golden Japanese’ plum and ‘Mit Ghamre’ and ‘Balady’ peaches were studied. The plants were grown in lysimeters. The growth of the trunk, total shoot length, the increase in shoot length per cm and the fresh weight of top, root and total plant were reduced with increasing salinity of the irrigation water. The effect was accentuated when the plants were maintained at high water table level. The salinity treatments resulted in the death of 43%, 73% and 76% of the plants in the plum, and the ‘Mit Ghamre’ and ‘Balady’ peaches, respectively, indicating that the plum is more tolerant to salinity than the peach. The plants of the salinity treatments showed various symptoms of salt injury, such as leaf burn, defoliation, shoot die-back and finally death. In the peaches, salt injury started to occur in the first growing-season, whereas the salt injury appeared in the plum in the second growing-season. The symptoms were more pronounced in the 4000 p.p.m. treatment than in the 2000 p.p.m. treatment and were more pronounced at the high water table level. The salinity level was the predominant factor and the effect of the water table on the vegetative growth diminished with increase in the salinity level of the irrigation water. 相似文献