Hyperostotic bone disease in red pandas (Ailurus fulgens).

Three adult red pandas (Ailurus fulgens) developed multiple periarticular exostoses in their elbow joints. Two of these animals also had extensive periosteal new bone formation and osteosclerosis of the ulnae and radii and mild periosteal new bone deposition on the femurs. One animal also showed extensive hyperostosis of the cranium and mandibles. Dietary concentrations of calcium and phosphorus were estimated to be adequate, but dietary vitamin A and D appeared excessive when compared with recommended levels for this species. Serum vitamin A concentrations were not elevated in the two most severely affected animals, but their liver vitamin A content was higher than what is considered normal for most domestic animal species. Serum 25-hydroxy vitamin D concentrations were within normal ranges for domestic species. A definitive diagnosis for the cause of the lesions was not established, but hypervitaminosis A was suspected.     

Serosurvey of ex situ giant pandas (Ailuropoda melanoleuca) and red pandas (Ailurus fulgens) in China with implications for species conservation.

Conservation strategies for the giant panda (Ailuropoda melanoleuca) include the development of a self-sustaining ex situ population. This study examined the potential significance of infectious pathogens in giant pandas ex situ. Serologic antibody titers against canine distemper virus (CDV), canine parvovirus (CPV), canine adenovirus (CAV), canine coronavirus (CCV), canine herpesvirus, canine parainfluenza virus (CPIV), Toxoplasma gondii, Neospora caninum, and Leptospira interrogans were measured in 44 samples taken from 19 giant pandas between 1998 and 2003 at the Chengdu Research Base of Giant Panda Breeding in Sichuan, China. Seroassays also included samples obtained in 2003 from eight red pandas (Ailurus fulgens) housed at the same institution. All individuals had been vaccinated with a Chinese canine vaccine that included modified live CDV, CPV, CAV, CCV, and CPIV. Positive antibody titers were found only against CDV, CPV, and T. gondii. Sera were negative for antibodies against the other six pathogens. Results indicate that the quality of the vaccine may not be reliable and that it should not be considered protective or safe in giant pandas and red pandas. Positive antibody titers against T. gondii were found in seven of the 19 giant pandas. The clinical, subclinical, or epidemiologic significance of infection with these pathogens via natural exposure or from modified live vaccines in giant pandas is unknown. Research in this area is imperative to sustaining a viable population of giant pandas and other endangered species.     

Dermatophytosis in red pandas (Ailurus fulgens fulgens): a review of 14 cases.

Fourteen cases of dermatophytosis were identified from medical records of red pandas (Ailurus fulgens fulgens) housed at the Knoxville Zoo between 1980 and 1996. The median age of affected animals on initial presentation was 8.5 wk (3 wk-11 mo). Clinical signs included crusting, purulent exudate, alopecia, thickening of affected skin, ulceration, and necrosis. Seven animals had mild lesions with signs restricted to crusting and/or alopecia, and six animals had more severe infections, with ulceration, skin necrosis, and purulent exudate. Five of the severely affected pandas had tail involvement. The severity of disease affecting one individual was not recorded. Dermatophytosis was confirmed by culture, cytology, histopathology, or culture followed by histopathology. Microsporum gypseum was the only fungal organism cultured. Six animals were treated for mild disease, and all clinical signs resolved. Partial tail amputation was required as part of the treatment regimen for two of the six severely affected animals, and two others had ulcerated tail lesions that left circumferential scarring after resolution of infection. Itraconazole (5 mg/kg p.o. q 12-24 hr) was the most frequently used systemic antifungal agent in animals with severe lesions. All fungal infections resolved, although one panda died from unrelated causes early in the treatment period.     

Tyzzer's disease in a red panda (Ailurus fulgens fulgens).

A debilitated 9-yr-old female red panda (Ailurus fulgens fulgens) with a recent history of corticosteroid administration displayed anorexia, depression, and diarrhea for 2 days. Blood work revealed a moderate nonregenerative anemia, leukocytosis, hypokalemia, hyperbilirubinemia, and mildly elevated alanine aminotransferase and aspartate aminotransferase. Serology was negative for occult heartworm, Toxoplasma gondii, feline leukemia virus, feline infectious peritonitis, feline immunodeficiency virus, and canine distemper virus. Electron microscopy of the feces demonstrated corona-like virus particles. The panda died 3 days after initial presentation. Histologic findings included multifocal, acute, hepatic necrosis and diffuse, necrotizing colitis. Liver and colon lesions contained intracellular, curved, spore-forming, gram-negative, silver-positive rods morphologically consistent with Clostridium piliforme. This panda most likely contracted Tyzzer's disease subsequent to having a compromised immune system after corticosteroid administration and concurrent disease.     

Avascular necrosis of the femoral heads in a red panda (Ailurus fulgens fulgens): possible Legg-Calve-Perthes disease.

A 17-mo-old captive-born female red panda (Ailurus fulgens fulgens) presented with a sudden onset of lameness in its left hind leg was diagnosed radiographically as having possible severe, bilateral Legg-Calve-Perthes disease with fracture of the great trochanter of the left femur. Surgical repair of the fracture was performed using pins and a tension band wire through a lateral approach to the hip. This is the first case reported at Madrid Zoo-Aquarium, where 63 individuals have been bred over 15 yr.     

Chromobacteriosis in a Chinese red panda (Ailurus fulgens styani).

An adult Chinese red panda (Ailurus fulgens styani) transported by airplane from Florida to a North Dakota zoo died 1 week after arrival. Grossly, an interscapular abscess, subcutaneous inflammation, lymphadenitis, and pulmonary abscesses were observed. Microscopic findings included necrotizing inflammation in liver, lung, lymph node, and spleen. Chromobacterium violaceum was cultured from the interscapular abscess, liver, lung, and spleen and was injected into Swiss Webster mice. These mice died 18 hours postinoculation, and C. violaceum was cultured from liver, lung, and spleen. Chromobacterium violaceum is a sporadically reported but highly virulent pathogenic bacterium of both animals and humans typically found as a soil and water inhabitant of tropical and subtropical regions.     

圈养小熊猫的疾病

小熊猫作为一种高度特化的素食性食肉目动物,在分类、系统进化及生态学等领域具有十分重要的研究价值,也是动物园里具有很高观赏价值的一种动物。为了更好地保护小熊猫免受疾病侵扰,做到有针对性的疾病预防,对近年来有关小熊猫疾病的报道进行了总结,并按病毒病、细菌病、真菌病、寄生虫病、代谢病、中毒病等进行了分类介绍。     

小熊猫的圈养繁殖

讨论了广州地区圈养繁殖小熊猫的技术要点,基于小熊猫行为研究的笼舍环境设计,以及圈养小熊猫的日粮组成和营养水平,提出了适度驯化有助于动物管理、生物安全防制疾病的观点.     

中国小熊猫生态学研究现状

概述了中国小熊猫的分布现状、栖息地状况,进一步阐明了目前对我国小熊猫的研究主要集中在生境和食物选择方面,且研究地区主要在四川进行,分析了我国小熊猫保护面临的受到人类活动的干扰、森林过度采伐、乱捕滥猎等问题,提出了加大投入、扩大保护面积、建立和实施保护补偿制度、提高公众保护意识以及加强科学研究等相应的保护对策。     

小熊猫人工育幼的研究

2007年7～10月,昆明动物园对1只小熊猫幼仔进行人工育幼,提供适宜的育幼条件,采用兔奶与人工乳制品相结合饲喂,终于获得成功.同时结合前几年昆明动物园小熊猫人工育幼失败的教训及其他动物园成功的经验,指出了小熊猫人工育幼成败的关键因素在于适宜的环境条件,合理的饲料配给,精心的护理,特别是饲喂适合的动物乳汁可大大提高人工育幼条件下小熊猫幼仔的成活率.     

小熊猫的常规行为训练

对小熊猫进行常规行为训练的目的是减少人为应激对小熊猫的影响,方便兽医对小熊猫进行疾病预防、诊断与治疗,同时有助于了解小熊猫的生长状况,更是为了增进人和动物的和谐关系,提高动物福利.小熊猫的行为训练在正常饲喂时间进行,主要在小熊猫采食牛奶稀饭或苹果的过程中对其进行分步式、渐进式、强化式训练.通过1a时间的训练,动物园内饲养的4只小熊猫都能主动配合训练者完成肌注、采血、心率测定、体温测量、体重称量、眼睛检查.     

Mortality associated with melarsomine dihydrochloride administration in two North American river otters (Lontra canadensis) and a red panda (Ailurus fulgens fulgens).

Two adult North American river otters (Lontra canadensis) and an adult red panda (Ailurus fulgens fulgens) at three separate institutions died within 22 hr after receiving single 2.5- to 2.7-mg/kg doses of melarsomine dihydrochloride administered in the epaxial musculature as a treatment for filarid nematodes. One otter had a suspected Dirofilaria immitis infection, the other had a confirmed D. lutrae infection, and the red panda had a confirmed Dirofilaria sp. infection, presumably with D. immitis. Postmortem examinations revealed similar gross lesions, although they were less severe in the red panda. The trachea and primary bronchi contained abundant foamy fluid, the lungs were mottled with areas of consolidation, and the pulmonary parenchyma exuded abundant fluid at the cut section. Histologic evaluation revealed acute pulmonary edema, which resulted in respiratory failure and death. There may have been direct pulmonary cellular toxicity of melarsomine dihydrochloride or a severe systemic anaphylactic reaction to antigens released after parasite death. An idiosyncratic drug reaction or a low therapeutic index of melarsomine probably caused the death of the three individuals. Melarsomine dihydrochloride use should be avoided in North American river otters and red pandas.     

圈养条件下小熊猫指名亚种旱季的活动规律

2008年11～12月,在姚家坪小熊猫繁育基地采用目标取样法和瞬时扫描取样法对19只圈养小熊猫指名亚种的活动规律进行了研究。结果表明,圈养小熊猫1d大部分时间用于休息（56.2%）,其次是运动（25.1%）和采食（9.9%）,其余行为分配的时间较短。在行为节律上,休息行为除9：00～9：55外,其余时间均为所占比例最高的行为,出现4个休息高峰,分别为8：00～8：55、11：00～11：55、14：00～14：55和17：00～17：55,在9：00～9：55出现极低值16.3%,此时是运动和采食的高峰;运动行为在9：00～9：55、13：00～13：55和15：00～15：55形成波峰;采食有2个高峰,分别为9：00～9：55和19：00～19：55;标记行为在9：00～9：55、10：00～10：55和13：00～13：55出现频率较高,超过5%,修饰行为在9：00～9：55超过5%,其余时间标记和修饰行为均低于5%;其他行为所占比率较低。不同年龄组间小熊猫的行为时间分配有差异,老年在休息行为上明显高于幼年和成年,约占总行为的72%;幼年在休息、运动和标记行为上略高于成年;成年的修饰行为明显高于幼年和老年。     

圈养小熊猫繁殖初探

建立小熊猫人工种群开展繁殖研究是减轻野生种群压力,有效保护小熊猫的措施之一。为探索提高人工条件下小熊猫繁殖水平,2011年以来成都大熊猫繁育研究基地对圈养小熊猫的环境条件等进行了改进提高。通过对小熊猫的饲养、繁育环境条件的改善,小熊猫的繁育情况有了一定的提高,初步结果显示,改进后,小熊猫的发情率、受胎率没有显著变化,但其繁殖成活率从改进前的55. 5%极显著地提高到改进后的76. 26%(P <0. 01)。     

小熊猫接种犬瘟热弱毒疫苗的效果分析

使用荷兰英特威(intervet)公司生产的犬用犬瘟热(Canine Distemper)弱毒疫苗,接种了云南野生动物收容拯救中心、云南野生动物园和南京市红山森林动物园3处不同地点、不同饲养环境条件下的72只小熊猫,历时12 a,先后共接种302次,接种前所有小熊猫经过检疫、检查,无任何疾病,健康状况良好.抽检其中4只小熊猫作抗体变化测定,接种前中和抗体效价平均为1∶44,经犬用犬瘟热病弱毒疫苗接种后中和抗体效价提高到1∶1976,经多次接种试验,除1只发生不良反应出现死亡之外,其余接种的71只健康状况良好,多年来未发生犬瘟热病,通过此次试验,证明使用荷兰英特威公司生产的犬用犬瘟热弱毒疫苗接种小熊猫较为安全,中和抗体效价比接种前提高了45倍,有很好的免疫效果,是国内动物园预防小熊猫犬瘟热较为理想的疫苗.     

小熊猫源大肠杆菌的药敏特性及致病性

为探明死亡圈养小熊猫(Ailurus fulgens)体内分离大肠杆菌(Escherichia coli,MT820501)的耐药情况及致病性,对分离菌株进行药敏特性分析,同时以小鼠感染实验检测其致病性。结果显示:该分离菌株对哌拉西林、头孢西丁、头孢曲松、头孢吡肟、美罗培南、哌拉西林-他唑巴坦、氨苄青霉素-舒巴坦、卡那霉素、丁胺卡那、新霉素、米诺环素、左氧氟沙星、呋喃唑酮、多黏菌素B敏感,对大部分β-内酰胺类药物及少部分氨基糖苷类药物耐药;小鼠感染实验结果显示,分离菌株半数致死量为8.42×10⁷cfu/m L,死亡小鼠体内分离出与小熊猫体内一致的菌株,病理表现为各实质脏器出现不同程度水肿、充血及炎性细胞浸润,与死亡小熊猫病理损伤特点高度相似,但不完全相同,这可能与感染方式、种属差异、耐受性等相关。     

小熊猫奇异变形杆菌病的诊治

20 0 2年 4月 2 1～ 2 7日 ,广东某地饲养的 4只小熊猫(Ailurus fulgens)先后感染奇异变形杆菌 (Proteus.mirabilis)。由于发病急、病原菌株存在耐药现象 ,仅 2只经治疗而康复。1　临床症状发病小熊猫两雌两雄 ,均已成年 ,合群饲养 ,生活于当地多年 ,无既往病史。小熊猫发病初期表现为精神不振 ,爬卧于树枝上 ,不愿下来采食 ;口鼻分泌物增多 ,两前肢不断抹擦口鼻部 ,眼结膜潮红、充血 ,但无眼屎 ;体温 3 7 5～ 3 8℃。 2～4d后小熊猫出现稽留高热 (体温 3 9 3～ 40 5℃ ) ,精神沉郁 ,呼吸急促 ,听诊肺部有明显音 ;行走时后肢无力 ,左右…     

小熊猫卵巢肥大细胞与雌性生殖干细胞的初步观察

为了解小熊猫卵巢肥大细胞分布情况,收集15只成体小熊猫的卵巢,按常规组织学技术制作组织切片。根据甲苯胺蓝染色结果,肥大细胞主要分布于血管壁与卵泡动脉外膜的结缔组织中,其数量随着发情周期呈规律性变化,发情期最高,乏情期次之,妊娠期数量最少,不同时期之间肥大细胞数量差异极显著(P0.01);阿尔新蓝-番红花红(AB-S)染色将肥大细胞可分为黏膜型、结缔组织型与混合型。肥大细胞可能对小熊猫的受精,胚胎着床,妊娠有调节作用。免疫组化染色显示,小熊猫卵巢有组胺阳性细胞,这些细胞分布也是随着发情周期呈规律性变化,卵母细胞和生殖上皮细胞呈现小鼠脉管同系物(MVH)阳性反应,说明小熊猫卵巢内存在生殖干细胞。