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1.
The use of the scanning electron microscope gave a three dimensional representation of the epithelial surface. Additionally, light microscopy revealed the representative structure of the epithelium. The rete testis showed a single layer of cubic epithelial cells. Short and dense microvilli were found on the surface. Sporadically a single, cilia-like structure was recognized. An extratesticular rete testis was identified. The flowing transition of the epithelium between the rete testis and the efferent ductuli occurred at different levels, so that both kinds of epithelial structures were recognized in the same area. The efferent ductuli were composed of a single columnar epithelium consisting of two cell types, principal cells and ciliated cells. The ciliated cells were recognized by their cilia protruding into the lumen. The principal cells showed microvilli on their surface and bleblike apical protrusions which erupt into the lumen.  相似文献   

2.
In this study, the epididymal region of the Sudani duck was investigated using histological and lectin histochemical methods. Morphologically, the epididymal region of the Sudani duck is composed of extratesticular rete testis, proximal and distal efferent ductules, a short connecting duct, and epididymal ducts. Morphometric analysis of the epididymal region of Sudani duck revealed that the efferent ductules predominate in relation to the epididymal ducts. The distribution of sugar moieties within the epididymal region of the Sudani duck was investigated using ten different fluorescein isothiocyanate (FITC) conjugated lectins. In the rete testis epithelium, only PHA-L showed a positive reaction. Efferent ductules in contrary exhibited a wide range of lectin affinity whereas six positive lectins (Con A, LCA, PNA, WGA, PHA-L, PHA-E) were observed. In the connecting and epididymal ducts, four lectins (Con A, WGA, PHA-L, PHA-E) were also detected. GSA-I, UEA-I, and LTA were at all not evident in the epididymal region of the Sudani duck. In conclusion, the correlation between the large areas of the epididymal region occupied by the efferent ductules and the wide range of sugar affinity of this portion may confirm the speculation that efferent ductules might be the primary site of fluid reabsorption in the epididymal region of Sudani duck.  相似文献   

3.
A surgical technique was developed to collect testicular fluid from the extratesticular rete testis of the pony stallion. A cannula was inserted through an incision in the visceral vaginal tunic following ligation of the efferent ductules. The cannula was sutured in place in the extratesticular rete testis. The other end of the cannula was exteriorized and inserted into a plastic collection receptacle fastened to the scrotum. Testicular fluid was collected for 12 to 36 hours at a rate of 1.07 ± 0.8 ml/hr. Sperm clots in the proximal end of the cannulas were responsible for the cessation of drainage.  相似文献   

4.
The early development of the gonads, and the ontogenesis of the rete testis and tubuli seminiferi recti in the chicken (Gallus domesticus) The primordium of the gonads can be discerned in the 4-day chick embryo by the elevation of the celomic epithelium to form germinal epithelium, and by the arrival of the primordial germ cells. Already in the 4½ day chick embryo there appears on the left side of the body, as a result of the first proliferation of the germinal epithelium, a subepithelial mesenchymelike cell conglomeration, which has been erroneously labelled in the literature a “urogenital union”. In contrast to the opinion expressed in the literature such a union does not appear until the 10th day, when the mesenchymal cells change to forerunners of rete cells which after hatching differentiate into rete-epithelial cells. The rete testis consists of intra and extratesticular transverse cisterns and of an intercalated and partically subdivided longitudinal cistern. The tubuli seminiferi contori end on the intracapsular lingitudinal cisterns either directly, or indirectly by intercalated tubuli siminiferi recti or intratesticular transverse cisterns.  相似文献   

5.
The rete ovarii is the homologue of the rete testis. It develops from cells of mesonephric origin which immigrate into the developing gonad of the embryo. The mature form of the rete ovarii is generally found to be groups of anastomosing tubules lined by cuboidal or columnar epithelium. These tubules are usually located in the hilus of the ovary, but may extend through the medulla or be isolated in the mesovarium adjacent to the hilus. The rete is often continuous with the transverse ductules through which it contacts the longitudinal duct of the epoophoron. The rete ovarii is important in the control of meiosis in the maturing ovary. Cells of the rete ovarii differentiate to form granulosa cells as well. The rete is also credited with secretory capability, a hypothesis supported by the observation of secretory material in the lumina of the rete tubules in several species. Cysts have been observed in the rete ovarii of several species. The rete ovarii of the adult does not appear to be a functionless vestige as has been previously reported.  相似文献   

6.
Effects of ligation of the ductus deferens on the fowl epididymal region   总被引:1,自引:0,他引:1  
The effects of ligation of the ductus deferens on the epididymis in the fowl were studied histochemically and immunohistochemically to reveal the mechanisms of sperm disposal. At one week post-ligation, the lumina of the rete testes (RT) and the efferent ductules (ED) were distended and filled with densely accumulated spermatozoa. Macrophages and foreign-body giant cells were aggregated in and around the accumulations. The epithelium regressed in the initial portion of the RT with the invasion of fibroblasts and heterophiles into the lumen. The other part of the epithelium was penetrated by many spermatozoa. Numerous lymphocytes and plasma cells infiltrated into the interstitium. At 4 weeks, larger number of spermatozoa agglutinated in the lumen, and large masses of foamy cells and proliferated connective tissue protruded into the lumen. At 8 weeks, large masses of foamy cells were noted. The connecting ductules or the epididymal duct showed no marked changes after ligation. The epithelium of the ED showed weaker or no acid phosphatase activity after ligation. Immunoglobulin G-containing cells increased in number in the interstitium. These results showed that ligation of the ductus deferens in the fowl causes granuloma in the RT and ED, and that epithelial cells, macrophages and granuloma are engaged in the removal of spermatozoa. The participation of antibody is suggested in the sperm disposal processes.  相似文献   

7.
The rete testis (RT) in the cat comprises three distinct and sequential parts: septal or adlobular; mediastinal and tunical parts. This last part is followed by the small extratesticular rete testis. The whole of the RT tubules and/or channels is lined by single low columnar and/or cuboidal epithelium with ultrastructural peculiarities of secretory and absorptive functions. The Golgi apparatus polarity (cis-trans surfaces), its relation with the long profiles of the ER, mainly with the rough ER, and presence of apical uncoated electrondense granules are suggestive of active release of macromolecules, probably glycoproteins. The absorptive function is proposed through the observation of a conspicuous microvacuolar-vesicular apical complex in epithelial cells. Moreover spermatozoa phagocytosis is also noticed, mainly in the septal part of the RT.  相似文献   

8.
A histochemical study using fluorescein isothiocyanate (FITC)-labelled lectins to identify glycoconjugates present in the efferent ductules and the three segments of the ductus epididymis (initial, middle and terminal segment) of dogs was carried out. The lectins used were: mannose-binding lectins (Con A, LCA and PSA), galactose-binding lectins (PNA, RCA), N -acetylgalactosamine-binding lectins (DBA, SBA, SJA and GSL I), N -acetylglucosamine-binding lectins (WGA and WGAs), fucose-binding lectins (UEA) and lectins which bind to complex carbohydrate configurations (PHA E and PHA L). The lectin-binding pattern in the canine epididymis presents similarities and differences to those observed in other mammalian species. The ductuli efferentes distinctly stained with most of the lectins used, whereas in the ductus epididymis a segment specific staining pattern was observed. Whereas principal cells of the ductus epididymis stained clearly with several FITC-labelled lectins (WGA, UEA and PHA-L), basal cells showed only a significant binding of Con A.  相似文献   

9.
The arrangement, origin, course and opening of the ductuli efferents testis of the bull (Bos taurus) were visualized using scanning electron microscopy. The corresponding capillary structure was also described and documented with the help of corrosive microanatomical casts. The number of the ductuli efferents testis of the bull varies narrowly between 12 to 13. Ductuli removed from the surrounding tissue have a length of up to 78 cm. The duct system is composed of linear as well as tortuous parts, permitting division into three distinct segments. Blind ending ductules are very short. We therefore do not see these structures as being associated with spermiostasis. The capillary density as well as the capillary architecture show regional peculiarities. All segments of the ductuli efferents testis are composed of capillaries of the continuous type. The findings indicate that the increased capillary density in the voluminous initial section of the ductuli efferents testis represents the basis for resorptive as well as secretory activities in the bull.  相似文献   

10.
The functional morphology of the rete testis, tubuli recti and terminal segments of the tubuli seminiferi in the mature bull The terminal portion of the seminiferous tubule in the mature bull has three segments: a transitional segment, a middle segment and a terminal plug. The middle segment and the terminal plug are formed almost exclusively by modified sustentacular cells. Each terminal segment is surrounded by a cuff-like, vascular plexus. The seminiferous tubule is succeeded by the straight tubule. The latter begins with a funnel-shaped enlargement which surrounds the terminal plug and then forms a smooth-walled, narrow tube that could be likened to the stem of the funnel. The tubular segment between the stem of the funnel and the rete is characterized by intraepithelial invaginations. The single layer of epithelium of the straight tubule and of the rete contains large numbers of free cells (lymphocytes and macrophages) which constitute up to 25 % of the epithelium. In the subepithelial stroma of the rete are free elastic fibers and flat cells which, because of their phosphatase content, are thought to be contractile.  相似文献   

11.
观察了5只健康成年母鸡腺胃粘膜上皮及其固有层腺体的亚显微结构。结果,粘膜表面散布许多隆起的乳头,其上皮为单层柱状上皮,上皮细胞顶端呈球状冠,表面布满球形微突;浅层单管腺上皮细胞形态与粘膜上皮细胞相似;深层复管腺的分泌导管开口于乳头顶端。复管腺由许多圆形或多形性小叶组成,每个腺小叶包括1个集合窦和许多围绕集合窦呈辐射状排列的腺小管。腺小管上皮细胞游离端呈椭球状冠。  相似文献   

12.
(1) Immunohistochemical methods and three antibodies (against actin, desmin and smooth muscle actin) were used to demonstrate the myoid cells in the domestic fowl testis and its excurrent ducts. (2) A positive reaction to actin, smooth muscle actin and desmin was found in the myoid cells of peritubular tissue of the testis and in rete testis, ductuli efferentes and ductus epididymidis. (3) In the testis myoid-reactive cells form a single layer. In the rete testis, ductuli efferentes and the ductus epididymidis reactive myoid cells form a main component of the stroma. (4) Positive reaction to actin, smooth muscle actin and desmin was also observed in the myoid cells of the tunica albuginea and in the wall of blood vessels in the testis and epididymis, indicating a contractile function for the testicular capsule.  相似文献   

13.
Junctional complexes of the epithelia lining the rete testis, efferent ductules, connecting ductules and epididymal duct in the fowl were examined by transmission electron microscopy and by a tracer method using lanthanum nitrate. The junctional complexes were composed of tight junctions, adhering junctions and desmosomes. In the rete testis, one or two points of membrane fusion were observed at the tight junctions. In the efferent and connecting ductules and epididymal duct, the tight junctions consisted of a series of punctate membrane fusions. The adhering junctions and desmosomes showed no remarkable structural differences among these excurrent ducts. Vascularly infused lanthanum nitrate penetrated into the tight junctions of individual epithelia for variable distances, but was prevented from entering the lumen at the site of membrane fusion. These results suggest that the tight junctions can restrict the diffusion of materials via the paracellular route, and that they play an important role in maintaining a suitable fluid environment within the excurrent ducts.  相似文献   

14.
The luminal appearance of the various ducts of the epididymis of the ostrich was studied by scanning electron microscopy in tissues fixed by immersion in glutaraldehyde. The ductal types were similar to those previously described for some other species of birds. Numerous short microvilli, as well as a single cilium, projected from the apical surface of the rete testis cell. The ciliated cells of the efferent ductules projected tufts of cilia into the ductal lumen, while the non-ciliated cells bore short microvilli. The connecting and epididymal ducts were lined by a columnar cell type whose apical surface bore uniformly distributed microvilli and a single, centrally situated cilium. The spermatozoa found in all ducts of the epididymis bore a distal cytoplasmic droplet. This observation has implications for the maturational process in the ostrich spermatozoon in the epididymis. The surface features of the ducts, except for a few noteworthy differences, were generally similar to those previously described for the male domestic fowl, turkey and duck.  相似文献   

15.
The aim of the study was to answer the open questions concerning the development of the horse's testis. This study revealed that the seminiferous tubules originate from the sex cords of the coelomic epithelium and Leydig cells from the proximal part of mesonephric nephrones, whereas the rete and the ductuli efferentes derive from intermediate and distal parts of the mesonephric tubules. During the development the Leydig cells undergo an enormous proliferation due to the PMSG secretion in the mare. The proliferation of these cells prevent the deep penetration of the rete into the medulla and is therefore the reason for the reduced extension of the rete and mediastinum testis in the stallion, although 80% of these cells degenerate in the last third of pregnancy. The growth of the seminiferous tubules during sexual maturity reduces the rete to the extremitas capitata of the testis.  相似文献   

16.
In a study of the absorptive activities of the excurrent ducts of the testis of birds, six adult cocks of the Ovambo breed of domestic fowl were deeply anaesthetised and intravascularly perfused with buffered 3 per cent glutaraldehyde. Epididymal tissue was prepared conventionally for electron microscopy. In favourable sections in three of these birds avid ingestion of spermatozoa was revealed in the non-ciliated (Type I) cells of the epithelial lining of the initial part of the proximal efferent duct, at and a little beyond its junction with the rete testis. No obvious defects were detected in luminal spermatozoa. The testicular excurrent ducts were neither distended nor obstructed, and mononuclear cell mobilisation was absent in both the ductal and periductal tissue. The significance of this observation with regard to the specific segment of the efferent duct system involved and how certain spermatozoa are identified for elimination from the duct lumen, must await precise studies.  相似文献   

17.
应用HE染色法和免疫荧光组织化学技术分别对小鼠生后不同发育阶段附睾组织结构及Crb3在不同发育时期附睾组织中的定位表达进行了研究。结果显示,附睾在4周龄时上皮为2层~3层的假复层纤毛柱状细胞,顶端纤毛细长;6周龄上皮细胞层数减少至1层~2层;8周龄附睾管上皮厚度增至最大,上皮细胞呈单层高柱状;12周龄后附睾管上皮开始变薄,呈单层柱状。免疫荧光染色结果显示,Crb3蛋白主要分布在附睾上皮柱状细胞的胞膜,在精子尾部有微弱的表达,这提示了Crb3不仅与附睾上皮细胞的极性建立和维持有关,而且对精子活力和血-附睾屏障的形成也可能具有潜在的影响。  相似文献   

18.
Four different aquaporins (AQP1, 2, 5 and 9), integral membrane water channels that facilitate rapid passive movement of water, were immuno‐localized in the excurrent ducts collected from sexually mature cats during orchiectomy. Aquaporins 1, 2 and 9, were immuno‐localized at distinct levels, whereas AQP5 was undetectable all along the feline genital tract. No immunoreactivity was present at the level of the rete testis with any of the antibodies tested. In the efferent ducts, AQP1‐immunoreactivity was strongly evidenced at the apical surface of the non‐ciliated cells, and AQP9‐immunoreactivity was shown at the periphery of both ciliated and non‐ciliated cells. Aquaporins 2 was absent in the caput epididymidis, either in the efferent ducts or in the epididymal duct. Otherwise, AQP2 was increasingly localized at the adluminal surface of principal cells from the corpus to the cauda epididymidis and more weakly in the vas deferens epithelium. The supranuclear zone of the epididymal principal cells was AQP9‐immunoreactive throughout the duct, with the exclusion of the vacuolated sub‐region of the caput and with higher reaction intensity in the cauda region. AQP1 was present in the blood vessels all along the genital tract. AQP1 was expressed also in the smooth muscle layer of the vas deferens. The tested AQP molecules showed a different expression pattern in comparison with laboratory mammals, primates and the dog, unique other carnivore species studied to date. The present information is possibly useful in regard to the regional morphology of the feline epididymis and correlated functions, which are still a matter of debate.  相似文献   

19.
Cysts of the rete ovarii system from 20 cats are described. The cellular morphology of these cysts varies because the normal rete ovarii in the cat consists of three anatomically distinct parts. Cysts arise in the ovarian medulla or at the tubal extremity of the ovary, expand into ovarian stroma rather than the mesovarium , and are lined by ciliated columnar to flattened epithelium. Their functional significance is unknown.  相似文献   

20.
A surgical technique was devised to collect rete testis fluid from 14 mature goats. The tubular portion of the extratesticular rete testis was cannulated where it penetrated the tunica albuginea of the testis under the center of the head of the epididymis. The tip of the cannula was sutured in place in the extratesticular rete testis and the opposite end was passed through the scrotum and into a plastic collection bottle. The bottle was glued to pillow ticking, which was sutured to the scrotum. Continuous flow of rete testis fluid occurred for 0 to 14 days and was usually terminated by a sperm clot in the cannula. The flow rate was 0.59 +/- 0.37 ml/hr.  相似文献   

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