共查询到20条相似文献,搜索用时 0 毫秒
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Berman DM Karhadkar SS Hallahan AR Pritchard JI Eberhart CG Watkins DN Chen JK Cooper MK Taipale J Olson JM Beachy PA 《Science (New York, N.Y.)》2002,297(5586):1559-1561
Constitutive Hedgehog (Hh) pathway activity is associated with initiation of neoplasia, but its role in the continued growth of established tumors is unclear. Here, we investigate the therapeutic efficacy of the Hh pathway antagonist cyclopamine in preclinical models of medulloblastoma, the most common malignant brain tumor in children. Cyclopamine treatment of murine medulloblastoma cells blocked proliferation in vitro and induced changes in gene expression consistent with initiation of neuronal differentiation and loss of neuronal stem cell-like character. This compound also caused regression of murine tumor allografts in vivo and induced rapid death of cells from freshly resected human medulloblastomas, but not from other brain tumors, thus establishing a specific role for Hh pathway activity in medulloblastoma growth. 相似文献
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Deregulation of Akt/protein kinase B (PKB) is implicated in the pathogenesis of cancer and diabetes. Akt/PKB activation requires the phosphorylation of Thr308 in the activation loop by the phosphoinositide-dependent kinase 1 (PDK1) and Ser473 within the carboxyl-terminal hydrophobic motif by an unknown kinase. We show that in Drosophila and human cells the target of rapamycin (TOR) kinase and its associated protein rictor are necessary for Ser473 phosphorylation and that a reduction in rictor or mammalian TOR (mTOR) expression inhibited an Akt/PKB effector. The rictor-mTOR complex directly phosphorylated Akt/PKB on Ser473 in vitro and facilitated Thr308 phosphorylation by PDK1. Rictor-mTOR may serve as a drug target in tumors that have lost the expression of PTEN, a tumor suppressor that opposes Akt/PKB activation. 相似文献
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Phytohemagglutinin: inhibition of the agglutinating activity by N-acetyl-D-galactosamine 总被引:5,自引:0,他引:5
H Berberg J Woodruff R Hirschhorn B Gesner P Miescher R Silber 《Science (New York, N.Y.)》1966,154(752):1019-1020
The effect of simple sugars on the agglutinating activity of phytohemagglutinin was studied. N-Acetyl-d-galactosamine selectively inhibits the agglutination of leukocytes and erythro- cytes by phytohemagglutinin. 相似文献
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Rabbits pretreated with aldosterone exhibited lessened response to the pressor agent angiotensin. This observation may help to explain the absence of hypertension in certain instances in which both the formation of angiotensin and the secretion of aldosterone are above normal. 相似文献
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【目的】探讨瘦素(Leptin)影响大鼠脂肪细胞perilipin、ADRP和TIP47 mRNA表达的分子机理。【方法】以体外培养的原代大鼠脂肪细胞为研究对象,用50 ng/mL Leptin及Leptin与JAK-STAT3通路抑制剂(AG490)、MEK通路抑制剂(U0126)、PPARgamma激活剂(罗格列酮(Ros))联合处理原代大鼠脂肪细胞3 h,提取总RNA,半定量(Semi-quantitative,SQ)RT-PCR法检测PPAR gamma、perilipin、ADRP和TIP47 mRNA的表达情况。【结果】阻断JAK-STAT3通路,可以减弱50 ng/mL Leptin对原代大鼠脂肪细胞perilipin mRNA表达的抑制作用(P<0.05),但加剧了Leptin对大鼠脂肪细胞ADRP和TIP47 mRNA表达的抑制(P<0.05);阻断MEK通路可以加剧Leptin对大鼠脂肪细胞perilipin、ADRP和PPAR gamma mRNA表达的抑制作用(P<0.05)。【结论】Leptin通过JAK-STAT3通路抑制原代大鼠脂肪细胞perilipin mRNA的表达。 相似文献
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Enhancement of flicker by lateral inhibition 总被引:9,自引:0,他引:9
Sinusoidal modulation of illumination on the compound eye of the horseshoe crab, Limulus, produces a corresponding variation in the rate of discharge of optic nerve impulses. Increasing the area of illumination decreases the variation at low frequencies of modulation, but unexpectedly enhances-or "amplifies"-the variation at the intermediate frequencies to which the eye is most sensitive. Both effects must result from inhibition since it is the only significant lateral influence in this eye. 相似文献
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Concentrations of butylated hydroxyanisole as low as 8 x 10(-9) mole per liter can inhibit detectably the contraction of smooth muscle elicited by bradykinin. The mechanism of the inhibitory effect of this food grade anti-oxidant is apparently complex, and the effect is only partially reversible. 相似文献
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[目的]探求应用RAPD技术区分麻蝇种类。[方法]用RAPD技术对黑尾黑麻蝇、棕尾别麻蝇、红尾粪麻蝇进行扩增,并对反应体系和程序进行一系列优化,从中选出可以区分3种麻蝇的引物和特异性条带。[结果]引物S247、S266对3种麻蝇扩增结果存在显著差异。[结论]RAPD技术是一种可以有效区分3种麻蝇的方法。 相似文献
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Growth inhibition by mechanical stress 总被引:2,自引:0,他引:2
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采用 RAPD 技术对 E.tenella 早熟株、鸡胚株、亲本毒抹和田间分离的抗药株进行研究,发现本研究选育的 E.tenella 早熟株与亲本毒株间 DNA 多态性存在着差异,证实该早熟侏已发生了遗传变异,但变异程度不大;而鸡胚株及抗药株却比早熟株的变异程度大。建议 RAPD 技术可用于艾美耳球虫株间差异及其球虫株间关系距离的检测.对经细胞培养的 E.acervulina 早熟株及其毒株和抗药株的基因组 DNA 进行多态性比较研究,发现该3株球虫间的变异程度大于 E.tenella 各虫株间的变异,即不同来源的 E.ac-ervulina 各虫株间的相似性小于 E.tenella 各虫株间的相似性,表明不同球虫其种内变异程度不一致。本研究结果表明 RAPD 技术可作为球虫株间变异的分子标记方法及其球虫株间关系距离的检测手段。 相似文献
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Activation of the protein kinase Raf can lead to opposing cellular responses such as proliferation, growth arrest, apoptosis, or differentiation. Akt (protein kinase B), a member of a different signaling pathway that also regulates these responses, interacted with Raf and phosphorylated this protein at a highly conserved serine residue in its regulatory domain in vivo. This phosphorylation of Raf by Akt inhibited activation of the Raf-MEK-ERK signaling pathway and shifted the cellular response in a human breast cancer cell line from cell cycle arrest to proliferation. These observations provide a molecular basis for cross talk between two signaling pathways at the level of Raf and Akt. 相似文献
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Rajendran L Schneider A Schlechtingen G Weidlich S Ries J Braxmeier T Schwille P Schulz JB Schroeder C Simons M Jennings G Knölker HJ Simons K 《Science (New York, N.Y.)》2008,320(5875):520-523
beta-Secretase plays a critical role in beta-amyloid formation and thus provides a therapeutic target for Alzheimer's disease. Inhibitor design has usually focused on active-site binding, neglecting the subcellular localization of active enzyme. We have addressed this issue by synthesizing a membrane-anchored version of a beta-secretase transition-state inhibitor by linking it to a sterol moiety. Thus, we targeted the inhibitor to active beta-secretase found in endosomes and also reduced the dimensionality of the inhibitor, increasing its local membrane concentration. This inhibitor reduced enzyme activity much more efficiently than did the free inhibitor in cultured cells and in vivo. In addition to effectively targeting beta-secretase, this strategy could also be used in designing potent drugs against other membrane protein targets. 相似文献
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对一元函数取对数求导法的两种常见类型之一——由若干因式的积、商、幂组成的函数的取对数求导作了进一步的讨论,对含有负因式、零因式时取对数求导也得出相应的结论。 相似文献
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