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1.
 为了探索小麦抗白粉病分子机理,明确茉莉酸对小麦白粉病抗性的诱导作用、对植物抗病性标志基因PR-1、PR-2、PR-5和本实验室克隆的1个新基因Ta-JA2的激活作用,以及抗病性变化与基因表达变化之间的相关性,本研究以感白粉病的小麦品种“中国春”、“濮麦9号” 和“周麦18”为材料,用茉莉酸甲酯(methyl jasmonate,MeJA)喷洒小麦幼苗叶片进行诱导,通过离体叶段培养法接种白粉菌(Blumeria graminis f. sp. tritici,Bgt)进行抗性鉴定;用实时定量PCR技术检测小麦叶片中PR-1、PR-2、PR-5和Ta-JA2基因的表达变化。结果表明MeJA处理可以显著提高“中国春”、“濮麦9号”和“周麦18”对白粉菌的抗病水平。茉莉酸处理显著激活了PR-1、PR-2、PR-5和Ta-JA2的转录。茉莉酸诱导的抗病性提高与抗病标志基因PR-1、PR-2、PR-5及Ta-JA2的表达增强呈正相关。植物激素茉莉酸是小麦抗白粉病反应的信号分子。  相似文献   

2.
Wheat (Triticum aestivum L.) powdery mildew has direct effects on photosynthesis- and energy-related pathways. Because it is prevalent in Sichuan Province, China, knowledge concerning Pm40-mediated wheat powdery mildew resistance is important for understanding host–pathogen interactions. The aim of this study was to understand physiological changes during the host–pathogen interaction, including gene expression, photosynthetic and chlorophyll fluorescence parameters, chlorophyll content, and antioxidant activity, specific to the resistance response to powdery mildew. The Pm40-expressing L693 and Pm40-deficient L1095 wheat lines were employed for comparison analyses. Fifty-eight and 26 expressed sequence tags (ESTs) were obtained from forward and reverse suppression subtractive hybridization cDNA libraries constructed from Bgt-infected L693 and L1095 leaves, respectively, and 69% of the ESTs in the forward library were related to photosynthesis- and energy metabolism-related pathways. Our data indicate that photosynthesis and carbon metabolism as well as nitrogen metabolism related to photosynthesis are the main pathways involved in Pm40-mediated wheat powdery mildew resistance and that an acetyl CoA-like gene might be a key regulatory factor in these pathways. This study provides new insight into the physiological and genetic mechanisms of resistance to wheat powdery mildew.  相似文献   

3.
The effect of soil solarization and Trichoderma harzianum on induced resistance to grey mould (Botrytis cinerea) and powdery mildew (Podosphaera xanthii) was studied. Plants were grown in soils pretreated by solarization, Tharzianum T39 amendment or both, and then their leaves were inoculated with the pathogens. There was a significant reduction in grey mould in cucumber, strawberry, bean and tomato, and of powdery mildew in cucumber, with a stronger reduction when treatments were combined. Bacillus, pseudomonad and actinobacterial communities in the strawberry rhizosphere were affected by the treatments, as revealed by denaturing gradient gel electrophoresis fingerprinting. In tomato, treatments affected the expression of salicylic acid (SA)‐, ethylene (ET)‐ and jasmonic acid (JA)‐responsive genes. With both soil treatments, genes related to SA and ET – PR1a, GluB, CHI9 and Erf1 – were downregulated whereas the JA marker PI2 was upregulated. Following soil treatments and B. cinerea infection, SA‐, ET‐, and JA‐related genes were globally upregulated, except for the LOX genes which were downregulated. Upregulation of the PR genes PR1a, GluB and CHI9 in plants grown in solarized soil revealed a priming effect of this treatment on these genes' expression. The present study demonstrates the capacity of solarization and T. harzianum to systemically induce resistance to foliar diseases in various plants. This may be due to either a direct effect on the plant or an indirect one, via stimulation of beneficial microorganisms in the rhizosphere.  相似文献   

4.
利用40个鉴别寄主对河南省5个小麦产区的44个单孢子堆纯化分离的小麦白粉病菌进行毒性鉴定分析,并采用多基因家系法进行了遗传多样性分析。结果显示,供试群体对Pm8、Pm1、Pm3a、Pm3b、Pm3c、Pm3f、Pm3e、Pm5、Pm6、Pm7、Pm17、Pm19、Pm34等抗病基因的毒性频率分别达到90%以上,已不能再作为抗源利用;对Pm12、Pm16、Pm21、Pm18、Pm2、Pm2+6、Pm2+MLD、Pm5(Mli)、Pm23、Pm30、Pm4a等基因的毒性频率低于15%,仍可以在抗病育种中加以利用;河南省的小麦白粉病菌存在地理之间的差异,同时病菌在地区之间有自西南向东北/东方向的传播。表明省内群体中存在着丰富的遗传多样性。  相似文献   

5.
Arbuscular mycorrhiza (AM) colonization led to a decrease in the severity of fusarium wilt disease caused by Fusarium oxysporum f. sp. lycopersici in tomato plants. The involvement of two plant defense hormones, namely methyl jasmonate (MeJA) and salicylic acid (SA), in the expression of mycorrhiza induced resistance (MIR) against this vascular pathogen was studied in the AM colonized and non-colonized (controls) plants. Activity of lipoxygenase (LOX), which plays a role in jasmonic acid (JA) biosynthesis, as well as levels of methyl jasmonate (MeJA) increased in AM colonized plants as compared to controls, but did not show any further changes in response to F. oxysporum inoculation. On the other hand, activity of phenylalanine ammonia lyase (PAL), which is an enzyme from salicylic acid (SA) biosynthetic pathway, as well as SA levels, increased in both controls and AM colonized plants in response to application of F. oxysporum spores. Hence the JA and not the SA signalling pathway appeared to play a role in the expression of MIR against this vascular pathogen. The resistance observed in AM colonized plants was completely compromised when plants were treated with the JA biosynthesis inhibitor salicylhydroxamic acid (SHAM). This confirmed that the AM-induced increase in JA levels was involved in the expression of resistance toward F. oxysporum. The SA response gene pathogenesis-related 1 (PR1) showed an increased expression in response to F. oxysporum infection in SHAM treated AM colonized plants as compared to plants that were not treated with this JA inhibitor. This suggested the possibility that JA inhibited SA responses, at least in the roots. AM colonization therefore appeared to prime plants for improved tolerance against the vascular pathogen F. oxysporum, which was mediated through the JA signalling pathway.  相似文献   

6.
Wheat powdery mildew, caused by the fungal pathogen Blumeria graminis f. sp. tritici (Bgt), is a destructive wheat disease worldwide. The key issue for the disease forecast is to timely and accurately estimate and quantify the latent infection levels in volunteer seedlings where the pathogen over-winters or over-summers to serve as sources of initial inoculum of epidemics. To improve the conventional method, a real-time PCR assay had been established in this study to quantify latent infection level of wheat leaves. Artificially and naturally infected leaves in wheat fields at different geographical locations in China were collected and processed to determine the latent infection levels. Linear relationships between the molecular disease index (MDX) and the observed disease index (DX) were obtained from artificial inoculation experiments. Field experiments showed that the spatial distribution patterns of MDX matched well with those of DX in the most cases. This study demonstrated that the real-time PCR assay was a useful tool to rapidly and accurately quantify the latent infection levels of wheat powdery mildew and to efficiently estimate the initial inoculum potentials of epidemics in the fields.  相似文献   

7.
为西北农林科技大学小麦新育成品种(系)在黄淮麦区的大面积推广,该研究对83份西农新育成的小麦品种(系)进行苗期抗条锈病和白粉病鉴定,成株期抗条锈病、白粉病、叶锈病和赤霉病鉴定,并在田间自然环境下对其抗性进行鉴定及对相关抗病基因进行分子检测。结果显示,在苗期人工接种鉴定中,有63、29和16份小麦品种(系)分别对条锈菌Puccinia striiformis f.sp.tritici生理小种CYR32、CYR33和CYR34表现出抗性,9份小麦品种(系)对3个条锈菌生理小种均表现出抗性;有10、3和0份小麦品种(系)分别对白粉菌Blumeria graminis f.sp.tritici生理小种E15、E09和A13表现出抗性。在成株期人工接种鉴定中,有23、15、28和62份小麦品种(系)分别对条锈病、白粉病、叶锈病和赤霉病表现出抗性。在83份小麦品种(系)中有6份在苗期和成株期均对小麦条锈病表现出抗性。在田间抗性鉴定中,有57、6、65和40份小麦品种(系)分别对条锈病、白粉病、赤霉病及叶锈病表现出抗性。在83份小麦品种(系)中,3份含有Yr5基因,22份含有Yr9基因,3份含有Yr17基因,2份含有Pm24基因,14份含有Lr1基因,所占比例分别为3.6%、26.5%、3.6%、2.4%和16.8%。  相似文献   

8.
Powdery mildew (Blumeria graminis f. sp. tritici) results in serious economic loss in wheat production. Exploration of plant resistance to wheat powdery mildew over several decades has led to the discovery of a wealth of resistance genes and quantitative trait loci (QTLs). We have provided a comprehensive summary of over 200 powdery mildew genes (permanently and temporarily designated genes) and QTLs reported in common bread wheat. This highlights the diverse and rich resistance sources that exist across all 21 chromosomes. To manage different data for breeders, here we also present a bridged mapping result from previously reported powdery mildew resistance genes and QTLs with the application of a published integrated wheat map. This will provide important insights to empower further breeding of powdery mildew resistant wheat via marker-assisted selection (MAS).  相似文献   

9.
10.
The effect of phytohormones on the defense response of wheat against Fusarium graminearum infection was investigated. Infection of heads with F. graminearum induced accumulation of salicylic acid (SA), jasmonic acid (JA), abscisic acid (ABA), and indole acetic acid (IAA). Exogenous phytohormone treatments showed crosstalk between them and a complex effect on expression of the genes ATB2, ExpB6, LEA Td16, PR1, Pdf1.2, PR4. JA treatment reduced F. graminearum growth and fusarium head blight (FHB) symptoms while an increase in FHB was observed with ABA. Transient down-regulation of allene oxide synthase (AOS) supports a complex role for JA in wheat head.  相似文献   

11.
This work is a detailed study of the infection of fungal biotrophic pathogens causing powdery mildew diseases on introgression lines originating from the intergeneric hybridisation between wheat and barley (Triticum aestivum L. × Hordeum vulgare L.). Powdery mildew fungi are among the most widespread biotrophic pathogens of plants also and infect dicot and monocot species. Most powdery mildew species are strictly host specific. They colonize only a narrow range of species or one particular host species. The intergeneric hybridisation between wheat and barley could result in expansions of host ranges of the barley powdery mildew. Our experiments covered natural infections in the field and artificial infections under greenhouse conditions. Formae speciales of powdery mildew were identified on the basis of the sequencing results of ribosomal internal transcribed spacer sequences (rDNA-ITS). We identified Blumeria graminis f.sp. tritici isolate 14 (HM484334) on the wheat parent and all wheat-barley introgression lines and B. g. f. sp. hordei isolate MUMH1723 (AB 273556) on the barley parent, respectively. The wheat-barley introgression lines were inoculated with barley powdery mildew under greenhouse conditions. According to our results the added barley chromosomes (or segments) do not cause host range expansion of barley powdery mildew.  相似文献   

12.
13.
Barley (Hordeum vulgare L.) is an important cereal crop and powdery mildew, caused by Blumeria graminis f. sp. hordei, is one of the most serious diseases that occurs on barley throughout the world. In the Middle East, which is the centre of diversity for barley and its pathogens, the wild barley–powdery mildew pathosystem co-evolves resulting in many specific resistances in the host as well as corresponding virulences in the pathogen. Many specific resistances have been used in European breeding programmes and a centre of pathogen diversity has arisen also especially in central Europe. This short review briefly summarizes the use of host resistances derived from wild barley and land races including the durable resistance gene mlo. The use of powdery mildew pathogenicity for studying new and unknown specific resistances and for identifying resistances in commercial varieties is described. However, highly heterogeneous wild barley is also characterized as a valuable source of minor genes for powdery mildew resistance. These might be exploited by barley breeders especially for winter barley improvement where the non-specific resistance gene mlo cannot be used.  相似文献   

14.
Upon pathogen or insect attack, plants respond with production of a specific blend of the alarm signals salicylic acid (SA) and jasmonic acid (JA), which are recognized as key players in the regulation of the signaling pathways involved. SA and JA responsive genes and SA/JA cross talk were well characterized in dicotyledonous species, but little is known in monocotyledonous plants. Using qRT-PCR, the expression profiles of SA and JA responsive genes were investigated after SA and JA treatments in monocots wheat. The results showed that Glu2 and PR-2 responded almost exclusively to SA, PR-3 and LOX2 responded positively to methyljasmonate (MeJA) treatment, while Lipase and PR-1.1 were induced in response to treatment with SA or MeJA. Furthermore, either by pathogen infection or exogenous application of hormones can activate the antagonistic effect between SA and JA in wheat, which has been well elucidated in dicotyledonous species. The outcomes of SA-JA interactions could be affected by the relative concentration of each hormone. This study shed light on marker genes that can represent SA and JA pathways in wheat and provided some clues for better understanding their interactions in monocot.  相似文献   

15.
16.
为了解河南省郑州市中牟市小麦全蚀病菌的变种类型及进化情况,筛选全蚀病菌侵染小麦的分子标记,采用形态学观察、科赫氏法则验证、ITS序列分析和系统进化树构建等方法对分离的小麦全蚀病菌进行鉴定,并对其侵染后小麦病原相关蛋白(pathogen-related protein,PR)基因的表达进行实时定量PCR分析。结果表明,显微形态观察初步确定分离的小麦全蚀病菌为禾顶囊壳Gaeumannomyces graminis(Sacc.)v. Arx.Olivier,经科赫氏法则验证、ITS序列比对及系统进化树分析进一步证明该病菌均为禾顶囊壳。本研究分离的小麦全蚀病菌与地域相距较远的英国、美国的小麦全蚀病菌间的同源性比与来自中国陕西省的小麦全蚀病菌的同源性更高。采用禾顶囊壳4个变种的特异性引物进行PCR扩增,所有分离病菌均扩增出了小麦变种的特异性条带,证实分离菌株为禾顶囊壳小麦变种G. graminis var. tritici。实时定量PCR分析发现,病原相关蛋白基因PR4a、PR4b、PR2、PR10受到小麦全蚀病菌侵染后表达量均显著上调,在侵染后第5~6天达到最高峰,表明这些基因可作为小麦全蚀病菌侵染小麦的标志性基因。  相似文献   

17.
When the biocontrol agent Pythium oligandrum (PO) colonizes the rhizosphere, it suppresses bacterial wilt disease in tomato (Solanum lycopersicum cv. Micro‐Tom) caused by Ralstonia solanacearum, and a homogenate of its mycelia exhibits elicitor activity, inducing an ethylene (ET)‐dependent defence response in Micro‐Tom. Since salicylic acid (SA) and jasmonic acid (JA) play an important role in plant defence responses to pathogens, the involvement of SA‐ and JA‐dependent signal transduction pathways in resistance to R. solanacearum was investigated in tomato roots treated with a mycelial homogenate of PO. Bacterial wilt disease was also suppressed in tomato cv. Moneymaker treated with the PO homogenate. However, the SA‐inducible PR‐1(P6) gene was not up‐regulated in either Micro‐Tom or Moneymaker. SA did not accumulate in homogenate‐treated roots in comparison with distilled water‐treated controls, even 24 h after inoculation. Induced resistance against R. solanacearum was not compromised in SA‐non‐accumulating NahG transgenic plants treated with the PO homogenate. On the other hand, the expression of the JA‐responsive gene for the basic PR‐6 protein was induced in both tomato cultivars treated with the PO homogenate. Furthermore, quantitative disease assays showed that the induced resistance against R. solanacearum was compromized in PO homogenate‐treated jai1‐1 mutant plants defective in JA signalling. These results indicated that the JA‐dependent signalling pathway is required for PO‐induced resistance against R. solanacearum in tomato.  相似文献   

18.
Powdery mildew, caused by Blumeria graminis f. sp. hordei (Bgh), is a worldwide disease problem on barley (Hordeum vulgare) with potentially severe impact on yield. Historically, resistance genes have been identified chiefly from cultivated lines and landraces; however, wild barley (H. vulgare subsp. spontaneum) accessions have proven to be extraordinarily rich sources of powdery mildew resistance. This study describes the characterization of a collection of 316 wild barley accessions, known as the Wild Barley Diversity Collection (WBDC), for resistance to powdery mildew and the genetic location of powdery mildew resistance loci. The WBDC was phenotyped for reaction to 40 different Bgh isolates at the seedling stage and genotyped with 10 508 molecular markers. Accessions resistant to all 40 isolates of Bgh were not found; however, three accessions (WBDC 053, 085 and 089) exhibited resistance to 38 of the isolates. Gene postulation analyses revealed that many accessions, while resistant, contained none of the 12 genes present in the Pallas near‐isogenic lines Mla1, Mla3, Mla6, Mla7, Mla9, Mla12, Mla13, Mlk1, MlLa, Mlg, Mlat and Ml(Ru2), suggesting that the accessions carry novel genes or gene combinations. A genome‐wide association study of powdery mildew resistance in the WBDC identified 21 significant marker‐trait associations that resolved into 15 quantitative trait loci. Seven of these loci have not been previously associated with powdery mildew resistance. Taken together, these results demonstrate that the WBDC is a rich source of powdery mildew resistance, and provide genetic tools for incorporating the resistance into barley breeding programmes.  相似文献   

19.
In this study, the Arabidopsis thaliana NPR1 (non‐expressor of PR genes) gene was integrated into an elite wheat cultivar, and the response of the transgenic wheat expressing NPR1 to inoculation with Fusarium asiaticum was analysed. With seedling inoculation, the transgenic lines showed significantly increased fusarium seedling blight (FSB) susceptibility, whereas floret inoculation resulted in enhanced fusarium head blight (FHB) resistance. Quantitative real‐time PCR revealed that expression of two defence genes, PR3 and PR5, was associated with susceptible reactions to FSB and FHB, whereas the PR1 gene was activated in resistance responses. This inverse modulation by the constitutively expressed NPR1 gene suggests that NPR1 has a bifunctional role in regulating defence responses in plants. Therefore, it is unsuitable for improving overall resistance to FSB and FHB in wheat.  相似文献   

20.
为明确中国61个小麦后备品种对白粉病的抗性水平及其抗病基因,将2016年从黄淮海冬麦区、长江中下游冬麦区及东北春麦区的9个市采集分离的269株单孢子堆白粉病菌,分别接种于61个小麦后备品种进行抗性测定;用NTSYSpc 2.10e软件对供试品种表型数据进行聚类分析;用35株鉴别菌株对29个含已知抗白粉病基因小麦材料和61个小麦后备品种进行鉴别,比较其抗谱并推导61个小麦后备品种所含抗白粉病基因。结果显示,61个小麦后备品种间的抗谱存在明显差异,国豪麦5号和7号、BL5008、绵麦系列、黔麦系列、楚麦16号、内麦101和366等18个品种抗谱较宽,抗性频率均大于97.0%;泰科麦5303、邯11-5272和临Y8222等10个品种的抗性频率在42.0%~56.1%之间;郑麦0943等33个品种的抗性频率小于37.9%。聚类分析可将61个小麦后备品种分成5大类,第I类有11个品种,其中8个品种的抗性频率在42.0%~56.1%之间;第II类和第III类共30个品种,抗性频率均小于32.7%;第IV类有2个品种,抗性频率分别为53.5%和53.2%,第V类有18个品种,抗性频率均大于97.0%;聚类显示来自于同一地区且抗性频率相近的品种具有相似或相近的抗性遗传背景。本研究推导出21个小麦后备品种含抗病Pm基因,其中,邯11-5272含有Pm30,安科1503含有Pm2、Pm5a、Pm6、Pm19和Pm30,临Y8222含有Pm5a、Pm6、Pm19和Pm30,云154-15含有Pm5a、Pm6、Pm7、Pm19和Pm2+ta,泰科麦5303等6个品种含有Pm2和Pm30,华麦7号等5个品种含有Pm5a、Pm6和Pm19,扬麦24号等6个品种含有Pm5a、Pm6、Pm19和Pm2+ta。研究表明,54.1%的小麦后备品种对白粉病菌群的抗性频率小于37.9%,存在适宜条件下小麦白粉病暴发流行的风险,因此这些小麦后备品种推广种植时需加强病害预警和监测。  相似文献   

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