IBA对东方杉硬枝扦插生根的影响

以2 a生东方杉扦插苗的1 a生硬枝为材料,研究了IBA对东方杉硬枝扦插生根的影响。结果表明:IBA对东方杉硬枝扦插生根效果显著,各处理生根率均比对照高,IBA 1000 mg/L水剂处理插穗60 s,其生根率达70.0%;而IBA粉剂对插穗生根的效果远好于水剂,尤其以1000 mg/L的IBA粉剂处理插穗生根效果最好,生根率达90.0%,平均不定根6.4条、平均根长7.2 cm。     

不同处理对软枣猕猴桃硬枝扦插生根的影响

通过设置不同激素及其浓度和不同插穗粗度处理对软枣猕猴桃进行硬枝扦插生根试验,结果表明,适宜浓度的IBA、APT对软枣猕猴桃硬枝扦插生根均有促进作用,用100 mg?L-1IBA激素处理12 h综合质量达到最佳,生根率达80.2%,平均根数12.23条,平均根长2.8 cm;插穗粗细对生根有影响,插穗直径为4.5~6.5 mm时生根效果最好。     

不同激素处理对丁香扦插生根的影响

采用IBA,ABT,NAA对丁香插穗进行处理,分析不同激素对丁香扦插生根特性的影响。结果表明,IBA处理硬枝插穗生根特性效果最佳,生根率最大值达到了84.63%;NAA处理嫩枝插穗生根效果最好,生根率最大值达93.32%。       

吲哚丁酸对青海云杉硬枝扦插生根效应的影响

介绍了青海云杉硬枝扦插插穗生根的三种类型,主要研究IBA(吲哚-3丁酸)的不同浓度和不同处理时 间对青海云杉硬枝插穗生根的影响,以及不同单株间插穗生根情况的差异。比较了IBA对青海云杉1年生硬 枝进行不同处理的生根差异、萌芽情况和插穗腐烂情况,找出了较好的促进青海云杉插穗产生更多高质量根 的．IBA浓度和处理时间的组合(IBA 250 mg·kg-1处理12 h);并且对IBA不同浓度,不同处理时间与插穗生 根之间的关系以及衡量扦插效果的不同指标间相关性均进行了分析,讨论了在IBA处理下插穗上部芽的萌发 与插穗生根之间的关系。     

乌饭树扦插技术研究

本文以乌饭树老枝、嫩枝和萌发枝为材料,研究不同插穗类型、不同扦插时间、不同扦插基质以及不同生长素处理插穗对扦插生根率的影响.结果表明,插穗类型以萌发枝生根率最高,即生根率76.1%;扦插时间以7月生根率较高,达到76.1%;扦插基质以过筛的黄心土的生根率最好,生根率为76.5%;不同生长素处理效果以生根粉1号处理的萌发枝插穗生根率最高,即77.4%;这些研究结果为乌饭树繁殖和产业化提供技术参考.     

钟花樱扦插繁育研究

比较不同基质、不同生根促进剂及其质量体积浓度、不同季节、不同修剪方式、不同插穗类 型对钟花樱（Cerasus campanulata）扦插生根的影响,结果表明：扦插基质类型、生根激素种类及其质量 体积浓度、扦插季节、插穗留叶面积以及插穗类型均对钟花樱扦插成活率和生根率产生显著或极显著影 响,扦插基质采用黄心土最有利于生根;生根激素种类及其各浓度处理效果均显著高于清水对照,其中 采用IBA 以质量体积浓度2 500 mg · L-1 速蘸10 s 处理效果最佳;于春季扦插最好;插穗留1 片或半片叶 片成活率及生根率远高于不留叶插穗;采用嫩枝扦插和带踵扦插优于硬枝扦插。因此钟花樱扦插宜在春 季,采用修剪成1 片或半片叶片带踵或嫩枝的插穗,经质量体积浓度2 500 mg · L-1 的 IBA 药液蘸10 s 后 扦插在黄心土上,生根效果最好。     

笃斯越桔的扦插技术研究

采用笃斯越桔嫩枝及硬枝扦插,用生根剂处理插穗,在不同的基质上进行扦插试验。结果表明,笃斯越桔扦插用河沙作为基质最好,生根剂ABT处理的插穗生根率最高,越桔嫩枝扦插以ABT 1mg/L、NAA 1mg/L和IBA 1mg/L效果最佳。笃斯越桔嫩枝扦插比硬枝扦插成活率高。     

IBA和ABT-1对新疆杨硬枝扦插效果的影响

为了筛选能提高新疆杨盐碱地扦插效果的药剂,研究了不同浓度IBA和ABT-1对新疆杨硬枝扦插效果的影响,结果表明:新疆杨插穗经IBA和ABT-1生根粉浸泡后,可有效促进扦插苗生根,缩短了生根时间,提高了生根率,提高了扦插苗的苗、径生长量;相同浓度ABT-1总体效果好于IBA效果;200mg·L-1 ABT-1处理对新疆杨的扦插效果最好。     

钟花樱扦插繁育研究

比较不同基质、不同生根促进剂及其质量体积浓度、不同季节、不同修剪方式、不同插穗类型对钟花樱(Cerasus campanulata)扦插生根的影响,结果表明:扦插基质类型、生根激素种类及其质量体积浓度、扦插季节、插穗留叶面积以及插穗类型均对钟花樱扦插成活率和生根率产生显著或极显著影响,扦插基质采用黄心土最有利于生根;生根激素种类及其各浓度处理效果均显著高于清水对照,其中采用IBA以质量体积浓度2 500 mg·L~(-1)速蘸10 s处理效果最佳;于春季扦插最好;插穗留1片或半片叶片成活率及生根率远高于不留叶插穗;采用嫩枝扦插和带踵扦插优于硬枝扦插。因此钟花樱扦插宜在春季,采用修剪成1片或半片叶片带踵或嫩枝的插穗,经质量体积浓度2 500 mg·L~(-1)的IBA药液蘸10 s后扦插在黄心土上,生根效果最好。     

长柄扁桃扦插繁育技术研究

以长柄扁桃为材料,研究了采用不同母株年龄的插穗、不同扦插时间以及不同浓度和不同时间的IBA处理对长柄扁桃嫩枝扦插生根率的影响。结果表明:随着母树年龄的增大,扦插生根率下降;萌芽抽枝后越早扦插,生根率越高;IBA处理能够提高长柄扁桃扦插生根率,浓度过高会抑制生根;IBA处理时间长短对扦插成活率没有影响;通过试验证明采集2年生母株上的当年生枝条作插穗,5月1日扦插,扦插时用800mg/L的IBA速蘸30s,扦插成活率可达81.25%。     

紫茎泽兰叶片浸提液对滇杨插穗抽梢和生根的影响

采用1年生滇杨扦插苗枝条作为插穗,研究了不同浓度紫茎泽兰鲜叶和冻害叶片浸提液对插穗抽梢和生根的影响。结果表明,滇杨在各处理条件下均能抽梢和生根,抽梢率和生根率在98％以上,生根类型为皮部生根。各处理条件下的平均梢长和平均不定根数达极显著水平,平均不定根长和根系效果指数差异不显著。紫茎泽兰鲜叶和冻害叶片浸提液对插穗抽梢及生根的化感作用表现一致,对梢长及不定根长有微弱的化感促进或抑制作用,对不定根数均表现出较强的化感抑制作用。     

无患子硬枝扦插进程插穗内源激素和多酚类物质含量变化

为了解无患子硬枝扦插生根机制,以1年生无患子硬枝作为扦插材料,在扦插第0—80天调查愈伤组织发育和根系形成情况,测定插穗的腋芽(嫩叶)以及基部2cm长度的韧皮部内源激素和多酚类物质的含量。结果表明,无患子硬枝扦插第10天开始出现愈伤组织,第50天愈伤率达到最高值83.33%;第40天开始出现生根插穗,生根率为6.67%,随后不定根数量迅速增加,第70天生根率达到86.67%,此时平均有5.33条。之后生根数量不变,根系仍然在生长,且扦插后根系效果指数持续增高。插穗韧皮部内源激素含量变化较复杂。整体来看,高含量赤霉素(GA3)抑制愈伤组织和不定根形成,且在根原基发生期和不定根形成关键期达到峰值,在不定根速生期持续下降;高含量生长素(IAA)促进不定根的形成,且在扦插后第20天,即根原基发生期,达到峰值(92.7μg/g);玉米素核苷(ZR)对无患子硬枝扦插过程的生理作用较复杂,低含量的ZR有利于根原基的发生和不定根速生,但高含量的ZR促进不定根形成。韧皮部ZR/IAA在扦插后第20—40天呈下降趋势,第40—60天快速上升,促进不定根生长;GA3/IAA整体呈现抛物线形下降趋势,特别是在根原基发生期和不定根速生期,下降速率更快,以促进不定根的生长。无患子硬枝插穗生根进程中,多数多酚类物质对愈伤组织形成、根原基发生及不定根形成有极显著抑制作用,没食子酸抑制效果稍弱;插穗内多种激素和多酚类物质的含量均发生了变化,且对插穗生根产生重大影响。总之,插穗根原基发生和不定根形成等关键时期,IAA含量升高,GA3、ZR以及多酚类物质含量降低,植物体内多种内源物质此消彼长,达到动态平衡,共同促进插穗生根。     

嫩江云杉扦插繁殖技术

通过露天网棚对分别来自于嫩江云杉和红皮云杉的嫩枝进行了扦插繁殖,试验结果表明：嫩江云杉可以通过扦插方式进行无性繁殖,其平均生根率为27．9％,插穗平均不定根数为6．2条;就插穗平均不定根数而言,嫩江云杉与红皮云杉无显著差异。但是,来自于40—59a生嫩江云杉树下的子代幼树的插稳的扦插生根率与红皮云杉相比较,达到显著水平,具有较高的扦插生根率;而60-99a生的嫩江云杉N-T的幼树的插穗的扦插生根率与红皮云杉相比较,无显著差异,表现出明显的年龄效应,即随着母树年龄的提高,其扦插生根率随之降低。     

植物扦插生根机理研究进展

植物扦插繁殖主要是不定根的形成过程,而不定根形成的复杂过程是多因素共同调控的,研究其生根机理对于了解不定根形成及控制具有重要意义。文中介绍了国内外关于不定根形成机理的分子水平、生理生化（植物激素、酶、营养物质等）以及解剖学等方面的研究进展。通过对生根机理的综合研究可以为难生根植物繁殖提供理论参考,为生产上加快植物扦插生根速率和提高其成活率提供技术支持。     

几种生根剂对金银忍冬嫩枝扦插育苗作用的试验初报

用IBA、NAA、ABT1、IBA＋NAA四种生根剂,分别以不同浓度,不同浸泡时间处理金银忍冬嫩枝插穗进行扦插育苗试验,结果表明：用IBA 1000×10^-6、处理2s、生根率最高为69.4%;用NAA 1000×10^-6、处理时间2s、生根率最高为90%;用ABT1 200×10^-6、处理时间2h、生根率最高为80.2%;用NAA 100×10^-6＋IBA 50×10^6、处理2h、生根率90%或NAA 50×10^-6＋IBA 100×10^-6、处理12h、生根率90%,用清水处理2h,生根率70.4%,表明用外源激素处理能够提高金银忍冬嫩枝扦插的生根率,增加不定根数量。从上面的方差分析结果综合生根率和生根性状的各项指标,如果选择一种激素,选择NAA 100×10^-6＋IBA50×10^-6、处理2h、生根率90%,效果最为理想。     

观赏桃嫩枝扦插技术研究

为了实现观赏桃的快速繁殖,以观赏桃品种‘元春’1年生半木质化的枝条为试材,分别就不同种类与浓度的植物生长调节剂处理、不同类型的基质处理对其扦插后的生根率、不定根数及不定根长度的影响情况进行了扦插试验。结果表明:以黄心土基质处理的生根效果优于以细河沙基质处理的;不同种类与浓度的生长调节剂处理对桃树生根的影响有差异,以浓度为1 000 mg/L的IBA处理的插穗其生根效果最佳,生根率为13.29%。文中综合分析试验结果后认为,适于桃树绿枝扦插的条件为:以黄心土为扦插基质,以浓度为1 000 mg/L的IBA溶液处理插穗。     

银杏枝条部位和年龄对不定根形成的影响及其与非结构碳水化合物含量的关系

[目的]研究银杏枝条部位、年龄对不定根形成的影响,并探讨其相应的叶、茎段中非结构碳水化合物(NSC)含量变化与扦插生根的关系。[方法]以50年生银杏树冠上、下部枝条的1、2和3年生位置的茎段为材料截取插穗,500 mg·L-1IBA与纯水对照处理基部60 s,在全光喷雾状态下研究扦插生根情况,并利用HLPC法分析相应部位叶和茎段中NSC含量。[结果]表明:银杏扦插不定根形成过程分为4个阶段,即0～4周愈伤形成期、4～6周愈伤膨大期、6～8周不定根开始形成期、8周后不定根伸长;生根部位主要在切口处。树冠上、下部位插穗的生根率未见明显差异,但随着枝龄的增加而明显降低。NSC分析表明:下部叶片NSC组分中多糖和总糖含量明显高于上部的,但茎段NSC的所有组分在上下部均无明显差异;随着枝龄的增加,相应位置叶片NSC各组分含量没有明显差异,但相应茎段NSC组分中葡萄糖、果糖、多糖、总糖、淀粉含量却明显降低,与生根率的变化趋势一致。此外,500 mg·L-1IBA处理并未明显提高生根率,还需进一步优化处理浓度。[结论]银杏树冠上部和下部枝条对不定根形成无影响;未处理枝条的枝龄与生根率呈负相关,而茎段NSC含量与生根率呈正相关;叶片NSC含量对不定根形成影响较小;生根方式以愈伤生根类型为主。该结论将为今后银杏扦插生根及其机制研究提供参考。     

Effect of branch position and auxin treatment on clonal propagation of <Emphasis Type="Italic">Tectona grandis</Emphasis> Linn. f.

The main objective of our clonal propagation program is the production of high quality timber and faster tree growth. Teak (Tectona grandis Linn. f.) is an important plantation species in the tropics but the demand of teak timber is increasing globally. Teak planting stock is still produced from seeds of unselected elite genotypes which may lead to poor stand establishment. The number of seeds per fruit and their germination ability are also variable and limited by several factors. Therefore an alternative method i.e., clonal propagation is required for mass multiplication of elite trees. It is an established fact that the clonal propagation or adventitious root formation in cuttings is markedly affected by several external and internal factors. Considering these, an experiment was conducted on leafy soft wood shoot cuttings of 3-year-old hedged teak stock plants grown in a hedge garden to study the effects of branch position and auxin treatment on adventitious root formation. A factorial completely randomized design was used for experimentation. Coppice shoots were collected from basal, middle and upper positions of the hedge garden; and mono-nodal leafy cuttings were prepared. These cuttings were treated with different concentrations of IBA and NAA; and were cultured in a mist chamber for rooting where the relative humidity was maintained at 85 ± 2% with maximum and minimum day-night temperature at 32 ± 1°C and 26 ± 1°C respectively. After 45 days the cuttings were removed from the rooting medium and observations were made. It was observed that the rooting parameters viz., percent rooting and percent sprouting, mean number of leaves, mean number of shoots and length, and mean number of roots and length per cutting were significantly influenced by the branch position and auxin treatment. Treatment with 4000 ppm IBA increased percent rooting and percent sprouting whereas NAA suppressed it. Treatment with 4000 ppm IBA also increased the mean number of leaves and shoots, and the length, and the mean number of roots per cutting. The highest mean number of roots and length were observed in cuttings taken from the branch emerging at the upper position followed by branches of middle and lower position. Cuttings of branches at a middle position showed the highest values of percent rooting, percent sprouting, mean number of leaves and shoots and mean shoot length per cutting. Furthermore, the interactive effect of branch position and auxin treatment on rooting and sprouting response was also found to be significant. Overall, the findings of this investigation suggest that middle branch position in hedge plants and 4000 ppm IBA can be made to induce maximum rooting and to produce high quality planting stock material for clonal forestry program of teak.     

THE OCCURRENCE AND DEVELOPMENT OF THE ADVENTITIOUS ROOTS FORMING GREEN SHOOT CUTTING OF LARIX OLGENSIS

The experiment with the green shoot cutting propagation of Larix olgensis wasmade in the Maoer Mountain Forestry Farm on July 5,1990.After the callus formed ofthe cutting base before adventitious roots came out the materials were collected from thecutting base and fixed in FAA solution.By the paraffin sections and anatomical observatio-n.the results indicted that it needed 40 days from the cutting to the rooting.The inducedprimordium of the cutting was endogenous in origin,which came from:(1)vascularcambium of the callus,and became roots;(2)meristematic cell population in the intersectbetween the cambium and xylem ray of the cutting base.There were extruding,crushingprocess and enzymolysis when the adventitious roots broke through the outer layers in tworooting types.     

The occurrence and development of the adventitious roots forming green shoot cutting ofLarix olgensis

The experiment with the green shoot cutting propagation ofLarix olgensis was made in the Maoer Mountain Forestry Farm on July 5, 1990. After the callus formed of the cutting base before adventitious roots came out the materials were collected from the cutting base and fixed in FAA solution. By the paraffin sections and anatomical observation, the results indicted that it needed 40 days from the cutting to the rooting. The induced primordium of the cutting was endogenous in origin, which came from: (1) vascular cambium of the callus, and became roots; (2) meristematic cell population in the intersect between the cambium and xylem ray of the cutting base. There were extruding, crushing process and enzymolysis when the adventitious roots broke through the outer layers in two rooting types.