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母猪子宫内膜炎病原菌的分离鉴定及药敏试验 总被引:1,自引:0,他引:1
《当代畜牧》2008,(1)
试验对广西11个猪场98份患子宫内膜炎的母猪子宫分泌物进行了细菌分离鉴定,分离到的细菌有链球菌、大肠杆菌、葡萄球菌、沙门氏菌,其中链球菌、大肠杆菌分别占53.85%和37.50%。对分离的主要细菌链球菌和大肠杆菌进行了药敏试验。 相似文献
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《黑龙江畜牧兽医》2015,(17)
为了了解山东、河北和河南等省部分地区养殖场鸡源致病性大肠杆菌的血清型及耐药性,试验采用细菌分离和生化鉴定方法对鸡大肠杆菌进行分离和鉴定,通过动物试验确定分离大肠杆菌的致病性,应用血清学方法和药敏试验对致病性大肠杆菌的血清型及耐药性进行研究。结果表明:分离鉴定的119株大肠杆菌中致病性菌株有110株,其中92株致病性菌株能够定型,分别属于15个血清型,有5株出现交叉凝集,O14、O35、O1、O2、O78、O88为优势血清型;分离鉴定的致病性大肠杆菌对环丙沙星、头孢噻肟、头孢哌酮等最敏感,对林可霉素、头孢克肟、卡那霉素、庆大霉素、红霉素的耐药率达60%以上。说明分离的致病性大肠杆菌血清型存在复杂性,对大部分临床常用药物产生了不同程度的耐药。 相似文献
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目的:从鸡舍的地表面分离大肠杆菌,试验研究大肠杆菌分离株的特征及其耐药性,为棚养鸡舍环境中大肠杆菌病的预防、控制和治疗提供资料。方法:从鸡舍表面和鸡舍地表下10cm土壤采集样本,采用分离培养、选择性培养、生化鉴定等方法,对分离的大肠杆菌进行鉴定;并通过药敏试验,了解大肠杆菌分离株对药物的耐药性。结果:本次从鸡舍表面和鸡舍地表下10cm土壤中采集的样本分离到的大肠杆菌对诺氟沙星、多种氨基甙类抗生素、红霉素、阿奇霉素和司帕霉素敏感,但对β内酰胺类的青霉素、甚至头孢唑啉、头孢哌酮却极不敏感。结论:从棚养鸡舍分离到的大肠杆菌,已对部分常规使用的药物产生了耐药性。 相似文献
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对临床发现的由鸡大肠杆菌引起的以鸡输卵管炎和腹膜炎为典型症状的传染病诊治情况进行论述。采用大肠杆菌分离培养、生化试验和药敏试验对病料进行了病原鉴定,结果表明徐州地区鸡群被大肠杆菌感染,并提出了防治鸡大肠杆菌病的建议。 相似文献
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XING Shou-ye ZHOU Xu-zheng LI Bing NIU Jian-rong WEI Xiao-juan ZHANG Ji-yu 《中国畜牧兽医》2016,43(1):256-260
The assay was aimed to study the sterility test and validation test of ivermectin microemulsion preparation and establish a sterility test method for ivermectin microemulsion preparation.The test method was carried out according to the method in volumeⅠ, Chinese Veterinary Pharmacopoeia Edition 2005.By choosing positive control bacteria and defining washing volumes in sterility test, the membrane-filter method was used to test the quantity of 10 bottles of test samples, and the sterility test was established.The result of method validation test showed that the test and all of positive control bacteria and microorganism growth after each filter being washed with 400 mL 0.1% peptone solution.It illustrated that the samples had no antimicrobial activity under the sample quantity and test condition.This method was available for sterility test of ivermectin microemulsion preparation.Using this method to test three lots test samples, the results showed that the positive control bacteria grew well within 24 h.The negative control bacteria and three lots test samples were sterile.It indicated that sterility test results met the requirements. 相似文献
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本试验旨在对伊维菌素微乳制剂进行无菌检查方法学验证和无菌检查试验,确认本试验所用的方法适用于该制剂的无菌检查。按《中国兽药典》2005版一部(附录118)所载"无菌检查法"项下进行试验,通过对阳性对照菌、不同量冲洗液等条件的选择,采用薄膜过滤法对10瓶供试品(每种试验菌的样品量)进行检测,建立了无菌检查方法。经方法验证,用400mL 0.1%蛋白胨水溶液冲洗后,含供试品容器中的7个阳性菌试验组与阳性菌对照组相比均生长良好,说明供试品的该检验量在该检验条件下无抑菌作用或其抑菌作用可以忽略不计,可以用该方法进行供试品的无菌检查。对3批供试品进行无菌检查,阳性对照菌均在24h内生长良好,阴性对照均澄清,无菌生长,3批供试品均澄清,无菌生长,无菌检查试验结果符合规定。 相似文献
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Validation of the indirect fluorescent antibody and the complement fixation tests for the diagnosis of Theileria equi 总被引:1,自引:0,他引:1
Ogunremi O Georgiadis MP Halbert G Benjamin J Pfister K Lopez-Rebollar L 《Veterinary parasitology》2007,148(2):102-108
The indirect fluorescent antibody (IFA) test for Theileria equi was evaluated to assess test's suitability for the serological diagnosis of equine piroplasmosis, to provide performance parameters for the purpose of test validation, and to compare it with the complement fixation (CF) test. Using a protocol that included Evan's blue, the specificity of the IFA test was estimated at 99.0% for T. equi by the classical method of analysis, and 96.6% by the Bayesian method. The use of Evan's blue in the test protocol increased test specificity and contributed to an excellent test agreement between two collaborating laboratories (kappa = 0.96). Using Bayesian analysis, the sensitivity estimate for the IFA test was 89.2%. The CF test sensitivity and specificity estimates for T. equi were 63.1 and 96.4%, respectively, as determined by Bayesian analysis. The IFA test was more sensitive than the CF test but the specificity estimates were similar. 相似文献
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E C Feldman 《Journal of the American Veterinary Medical Association》1985,187(1):49-53
Twenty-one dogs with hyperadrenocorticism were studied. Six dogs had functioning adrenocortical tumors and 15 had pituitary-dependent hyperadrenocorticism. Each dog was evaluated, using endogenous plasma ACTH, ACTH stimulation, dexamethasone screening, dexamethasone suppression, and combined dexamethasone suppression/ACTH stimulation tests. The ACTH stimulation portion of the combined test was less reliable as a screening test in diagnosing hyperadrenocorticism than was the isolated ACTH stimulation test or the dexamethasone screening test. The dexamethasone suppression portion of the combined test was less reliable in distinguishing dogs with adrenocortical tumors from those with pituitary-dependent hyperadrenocorticism than was the endogenous ACTH or isolated dexamethasone suppression test. The combined test is not recommended for use. The ACTH stimulation test is the recommended screening test because of its diagnostic reliability and its subsequent importance as a base line in determining success of mitotane therapy. 相似文献
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一株鸡肾型传染性支气管炎病毒的分离与鉴定 总被引:1,自引:0,他引:1
从山东某鸡场疑似肾型传染性支气管炎病死鸡的肾脏中分离到一株病毒,经SPF鸡胚连续传代、血凝试验、鸡新城疫干扰试验、鸡胚矮小化试验、RT—PCR鉴定和动物回归试验,初步确定该病毒为肾型传染性支气管炎病毒,并命名为SD1105株。 相似文献
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An evaluation of the delayed-type hypersensitivity test for diagnosing brucellosis in individual cattle: a field study 总被引:2,自引:0,他引:2
A field study was conducted to evaluate the delayed-type hypersensitivity (DTH) test in diagnosing brucellosis in cattle, in particular the diagnosis of infection in individual cows. A total of 93 cows that were negative, suspect, or positive to the serum agglutination test (SAT), complement fixation test (CFT), or the milk ring test (MRT) were subjected to the DTH test. The cows were then slaughtered and the supramammary lymph nodes were collected for bacteriologic examination. In 989 cows the DTH test, MRT and serologic tests were negative. When the DTH test results were compared with bacteriologic results, 12 of the 93 cows with CFT titres greater than 1:200 tested negative in the DTH test while bacteriologic results were positive. The sensitivity of the DTH test (calculated on the remaining 81 cows) was 100%; the specificity was 83%. The sensitivity of the DTH test (calculated on 93 cows) was 81%; the specificity was 83%. The sensitivity and specificity of the DTH test correlated well with those of the CFT (86-83%). We conclude that the DTH test is very sensitive, and specific enough to diagnose brucellosis in individual cows. The DTH test should be used in combination with serologic tests in the diagnosis of brucellosis. 相似文献