Mykotoxinbildung während der Infektion der Weizenähre durch den pathogenen Pilz Fusarium graminearum

Fusarium graminearum is one of the most destructive pathogens of cereals and a threat to food and feed production worldwide. Infection especially of wheat leads to yield losses and mycotoxin contamination. Deoxynivalenol is a hazardous mycotoxin inhibiting the biosynthesis of proteins. Its production by the fungus is a prerequisite for the colonization of the wheat spike. Combining molecular genetics with bioimaging techniques allows a fascinating insight into the pathobiology of this cereal pathogen.     

Silikate im Pflanzenschutz: Elektrostatische Applikation und Abhängigkeit der Wirksamkeit von der Schichtdicke

Natural occurring amorphous silica is ubiquitous on the earth in soils and sediments. Both, natural derived silica and synthetically produced amorphous silica are used against stored product pests and parasites in animal houses. New formulations, active even under higher relative humidity's can be possible used against horticultural arthropod pests. Since many horticultural pests hide on the undersides of leaves and silica is a physical contact insecticide, an appropriate application method is needed. This paper reviews electrostatic application of natural derived amorphous silica to Brassica chinensis leaves. Due to the inherent high volume resistivity of silica powder, induction charging was ineffective for electrostatic powder coating. Corona charging involves a reliable current source, but typically is inefficient in charging the powder particles and creates a high density of free ions between the gun and grounded substrate as well as within the coating layer. These factors resulted in poor coating quality and inefficient particle deposition. The best leave coating was archived using tribo charging. Here we have been able to apply coatings with different thickness. While a silica coating of 25–50?μm in petri dish experiments resulted in a reduced mortality of the granary weevil, Sitophilus granarius (L.), there was no difference between mortality with coatings of 50–100?μm and 100–125?μm. Silica applications on B. chinensis leaves resulted within two hours in a 45% reduction of the CO₂-absorption. Absorption level stayed reduced over a period of seven days and was between 45 and 73% compared to the control. Even after removing silica dust using pressurized air CO₂-absorption was between 19 and 28% reduced. This might be the case because silica particles where so small that they could possible clog the stomata opening resulting in a reduced transpiration and photosynthesis rate.     

Möglichkeiten der gezielten Bekämpfung von Pyrethroid-resistenten Rapsglanzkäferpopulationen – Ergebnisse von Ringversuchen mehrerer Bundesländer

From 2006 to 2008, tests for regulating pyrethroid-resistant pollen beetle populations using new insecticidal agents were conducted in the states of Brandenburg, Saxony, Saxony-Anhalt and Thuringia. Reason for this test series were high yield losses in winter rape in some states in 2006. These losses were the result of unilateral licensing procedures focussing on class??II pyrethroids. Organophosphates, like chlorpyrifos-ethyl and chlorpyrifos-methyl, as well as class??I pyrethroids like etofenprox and bifenthrin were examined during the tests concerning their efficiency towards Meligethes aeneus. As a comparative agent, neonicotinoid based on thiacloprid was used. Mixtures of class??II pyrethroids with piperonylbutoxid were not admitted by the producers in 2008 despite good insecticidal effects due to economic reasons. Therefore, in 2008, insecticides with different mode of action agents were tested for pollen beetle control. Investigations at five different locations showed that pymetrozin (Plenum 50 WG) and indoxacarb (STEWARD) were identical in their efficiency as thiacloprid (Biscaya) against pyrethroid-resistant pollen beetle. Metaflumizone (Alverde), a semicarbazone, is not eligible for controlling M.??aeneus populations in both investigated formulations. In order to avoid further selection of neonicotinoid-resistent pollen beetle populations, the admittance of other insecticidal agents in the coming years is necessary. For that purpose, organophosphates and insecticides based indoxacarb and pymetrozin would be good alternatives.     

Bewertung der Pflanzenschutzintensität landwirtschaftlicher Betriebe im Rahmen des Umweltsicherungssystems Landwirtschaft

Zusammenfassung Der Verband Deutscher Landwirtschaftlicher Untersuchungs- und Forschungsanstalten (VDLUFA) Bonn bietet unter der Bezeichnung USL bundesweit eine Umweltverträglichkeitsbewertung landwirtschaftlicher Betriebe an und vergibt das Zertifikat Betrieb der umweltverträglichen Landbewirtschaftung, sofern die dafür geltenden Bedingungen erfüllt sind. Zu den 17 Prüfkriterien gehört auch die Bewertung der betrieblichen Pflanzenschutzintensität. Diese wird seit ca. zwei Jahren über den so genannten Behandlungsindex ermittelt, der in den letzten Jahren auf die spezifischen Bedürfnisse von USL (belegbare Datenbasis zur Absicherung der Justiziabilität) zugeschnitten worden ist. Das Verfahren ist von 2001 bis 2003 in zahlreichen Landwirtschaftsbetrieben getestet worden, darunter auch im Umwelttestbetriebsnetz Thüringen (25 Betriebe, 31 Bewertungen). Die Ergebnisse zeigen, dass mit dieser Methode aussagefähige sowie reproduzierbare und justiziable Ergebnisse gewonnen werden können.     

Beitrag zum Sortentyp und Merkmalskennung der Reifedynamik durch den phänologischen Indikator, dem Silomais-Reifeindex

Data from the Central German variety trials was evaluated, to with the Silage maize Ripeness-Index (SRI) the off-ripe-specific type in its significance and impact on selected parameters of the quality, the yield of silage and energy maize, as well as the appropriate feeding of ruminants with rations of higher maize proportions. The characterization of the ripe type on the basis of SRI is closely associated with statements to maize ripening, reproducibility of variety performance, site suitability and environmental conditions of production in the context of the Dynamic Ripening and Analysis system (DRA). The environmental stability of maize varieties characterizes the type of variety that exerts a dominant role over the ripeness level on the production of silage maize. The difference between type and productivity as well as feed value was demonstrated. For a location and physiological ruminant feeding of maize in relation to the type are been conclusions in the field of plant health, ground feeding uptake, structure impact and physiological grain hardness. As a result of this evaluation a comprehensive advantage of the environment stable variety type with slow ripening maize (residual) plant is to determine which cannot be evidenced and used with the present system of ripeness of use-specific classification.     

Maßnahmen zur Eindämmung des Brennnesseltyps der Schwarzholzkrankheit bei Weinreben (Vitis vinifera)

Since 2003 incidence of Bois noir disease (Stolbur Type I) in the area of Baden-Württemberg has rapidly increased. Hylasthes obsoletus, known vector cicada transmitting Bois noir disease from stinging nettle to grapevine, was found on stinging nettles located at vineyard walls or vineyard tracks. Phytoplasma of Stolbur Type I (host plant: stinging nettles) were also detected within samples of Hylasthes obsoletus. Incidence of Bois noir disease could be strongly reduced by either not mowing or destroying stinging nettles (host plant of Hyalesthes obsoletus) between June and August, the flight period of Hylasthes obsoletus, or by eradication of stinging nettles in winter. Fighting stinging nettles between November and March with Glyphosate resulted in a dramatic decrease of vector cicada. Pruning experiments of the extremely sensitive grape variety Lemberger and the variety Trollinger showed that survival of grape vines significantly improved compared to normal pruning in winter if the vines were cut off 10?cm above ground immediately after detection of the first symptoms. In contrast, cutting off vines 10?cm above ground in summer had no effect on survival rates of 40 year old grape vines of the variety Pinot noir. Furthermore, most of these old grapevines did not survive this dramatic pruning measure. Generally it is advised to cut off diseased plant material as soon as symptoms become visible.     

Studien zur Bekämpfung von Phytophthora ramorum an Rhododendron im Rahmen der Entwicklung eines Behandlungskonzeptes für Park- und Gartenanlagen

In this study dealing with the development of management strategies for P. ramorum in Rhododendron stands several plant protection and fertilizer products were tested for their suitability to control this pathogen on Rhododendron. Tests were conducted on young potted plants and abscised leaves, using the following products: phosphite (Nutri-Phite^® PK), copper hydroxide (Cuprozin^® liquid), Bacillus subtilis (Serenade^®), a mycorrhizal preparation, and a product based on ‘effective microorganisms’ (EMa). Although transmission of the pathogen from infected to healthy plants was successfully inhibited by phosphite, Cu hydroxide and B. subtilis, subsequent tests showed that only phosphite and Cu hydroxide had a significant effect on disease development. All other preparations based on antagonistic or growth promoting microorganisms had no preventive or curative effect. While B. subtilis tended to increase disease symptoms, the remaining products caused a slight reduction of disease severity only. In contrast, phosphite and Cu hydroxide almost completely inhibited infection by P. ramorum. Therefore, in field situations, the use of the ecologically safe, systemic phosphite against P. ramorum in Rhododendron stands appears to be reasonable and advisable. Along with other supporting measures within the framework of a management strategy, this will allow to prevent or slow down further spread of the disease.     

Erarbeitung eines Testverfahrens zur Bestimmung der Protektiv- und Kurativleistung von Fungiziden bei der Bek?mpfung des Erregers der Stemphylium-Laubkrankheit Stemphylium botryosum in der Spargelkultur (Asparagus officinalis L.)

The foilage of Asparagus officinalis L. has a large contact surface for different leaf diseases, which can lead to profits cuts, especially by the causal pathogen of Stemphylium leaf spot, Stemphylium botryosum. The maintaining healthy of the foliage is an important precondition to achieve an optimal harvest in the following year. Therefore, it is necessary to describe the efficacy of currently approved plant protection products against S. botryosum in order to optimize the fungicide applications. In the context of greenhouse trials with asparagus seedlings a method should be developed to evaluate the preventive and curative efficacy of fungicides. At the beginning, stable, repeatable and homogeneous infections of S. botryosum needed to be achieved. Ad hoc the factors plant age (weeks after sowing date), inoculum (density of conidia per ml and concentration of malt), infection conditions (period of high relative humidity) and day of rating, as well as their influence on the occurrence of the symptoms, were investigated. With the exception of the factor plant age, the other factors had a significant effect on the infection. For the trials to evaluate the efficacy of fungicides, the analysed factors were defined. The age of the seedlings was six weeks after sowing, the inoculum was composed of 1,00?×?10⁵ conidia per ml combined with an 0,5% malt concentration, as infection condition the ??tunnel system?? with periodically high relative humidity was chosen. 13 days past infection was the optimal day of rating. To apply the seedlings with the fungicides, the registered application rate was reduced to 1/5. The trials to evaluate fungicides were conducted with the fungicides Signum^®, Rovral^® WG and two different testing agencies. At four consecutive application days, the plants were covered with fungicides. In the following, the infestation degree of stems in percent was evaluated. Additionally, the used S. botryosum-Isolates were biomolecularly investigated and the QoI- and SDHI resistance state determined.     

Virusnachweis in Blattläusen

Potato virus Y (PVY) causes great economic losses in potato production world-wide. Concerning important it has replaced Potato leafroll virus (PLRV). Virus is transmitted by different aphid species in a non-persistent manner. During last years the developing of new effective methods for PVY monitoring, forecasting and detection in aphids is of increasing interest. Sensitive, rapid detection of virus in its natural vectors is of a great need to investigate the relationship between aphid migration and the spread of PVY. Simple diagnostic protocol for the detection of non-persistent Plum pox virus and semipersistent Citrus tristeza virus in aphids, proposed by Olmos et al. (2005) was probed to validate and estimate the efficiency of its applying for the detection of PVY in different aphid species too.     

Maiszünslerbekämpfung – Bekämpfung und neue Entwicklungen beim Trichogramma brassicae-Einsatz

The European corn borer Ostrinia nubilalis (Huebner) is a well-known and investigated pest of corn and sweet corn particularly in the southwest of Germany since a long time. Nevertheless the pest can still surprise scientists and farmers. The first occurrence of a bivoltine race of the European corn borer in South Badenia in the years 2006 and 2007 is remarkable. The European corn borer had to be controlled in the last year on an area of approx. 60.000 hectares in Germany. An important antagonist of this pest is the parasitoid Trichogramma brassicae Bezdenko (Hym., Trichogrammatidae), which is already used for over 30 years on a continuously rising acreage for the control of Ostrinia nubilalis. The biology of the pest and its parasitoid are recapitulated particularly with regard to the biological control. The flight activities of the European corn borer are supervised with light traps in Southwest Germany. The data were inserted into a central data base at the LTZ Augustenberg, office Stuttgart (at first in 2007). The data can be used by advisors and farmers. They determinate the optimal time for the introduction of Trichogramma brassicae and optimize the application of insecticides. The annual randomized monitoring of the Trichogramma quantities and partially also qualities by the former state institute for plant protection and nowadays the LTZ Augustenberg helped to supply the farmers with good Trichogramma material. The efficiencies of the Trichogramma introduction reach up to over 70%. With the insecticide STEWARD (active ingredient Indoxacarb) similar and partly better efficiencies can be obtained. With the necessity of controlling Diabrotica virgifera virgifera with insecticides problems for the use of Trichogramma can arise – this will be discussed.     

Arbeitsergebnisse aus der Projektgruppe ?Krankheiten im Getreide“ der Deutschen Phytomedizinischen Gesellschaft e. V.

In den Jahren 1989 bis 1992 wurde in bundesweit durchgeführten Feldversuchen eine Bekämpfungsschwelle für Blatt- und Ährenkrankheiten in unterschiedlich widerstandsfähigen Winterweizensorten überprüft. Nach vergleichbarem Muster lief die Überprüfung verschiedener Schwellenwerte in Winterroggen, Winter- und Sommergerste von 1992 bis 1995 ab.Mit von 1995 bis 1998 ebenfalls mehrfaktoriell angelegten Feldversuchen beteiligte sich die Projektgruppe an dem Vorhaben der Bundesanstalt für Landwirtschaft und Ernährung (BLE) Untersuchungen zum Einfluss unterschiedlicher Pflanzenschutzmaßnahmen auf den Mykotoxingehalt und den Fusarium-Besatz im Erntegut von Getreide (1997–2000). Ergänzt um Beiträge der Projektgruppenteilnehmer aus dem eigenen Forschungs- und Entwicklungsbereich wurden die Erkenntnisse der Projektgruppe zu diesem Themenkomplex im Rahmen eines Workshops im November 2002 vorgestellt und mit ca. 120 Fachleuten aus Ministerien, der Produktionstechnik sowie den vor- und nachgelagerten Bereichen diskutiert.     

Gentechnisch veränderte Bäume für eine nachhaltige,umweltverträgliche und ressourcenschonende Produktion von Holz für die Energiegewinnung

Genetically modified trees (GM trees) are long-living plants. In regard to their special ecological and economic importance deployment of genetically modified trees is controversial because of the possible risks they are involved.Commercial use of GM trees for example as a plantation culture is supported by proponents, if they were previously subjected to a comprehensive risk analysis and safety assessment. In contrast, many critics exclude a commercial use of GM trees completely. They demand to stop the research.In this paper, the possibilities of a sustainable, environmentally sound and resource-efficient use of GM trees are discussed. Arguments are given in favor of GM trees in respect to both, as a source of renewable raw material to cover an increasing demand for energy and chemical raw materials.Besides, arguments are given in favor of GM trees to mitigate effects of climate change in comparison to agronomical energy crops such as maize, sugar beet and cereals.Finally, an overview of an international project on risk assessment of GM trees (recently approved by the European Union) is given. The paper concludes with a presentation of requested and approved field trials of GM trees in the last five years in Europe.     

Arbeitsergebnisse aus der Projektgruppe „Krankheiten im Getreide“ der Deutschen Phytomedizinischen Gesellschaft e. V.

Zusammenfassung In den Jahren 1989 bis 1992 wurde in bundesweit durchgeführten Feldversuchen eine Bekämpfungsschwelle für Blatt- und Ährenkrankheiten in unterschiedlich widerstandsfähigen Winterweizensorten überprüft. Nach vergleichbarem Muster lief die Überprüfung verschiedener Schwellenwerte in Winterroggen, Winter- und Sommergerste von 1992 bis 1995 ab.Mit von 1995 bis 1998 ebenfalls mehrfaktoriell angelegten Feldversuchen beteiligte sich die Projektgruppe an dem Vorhaben der Bundesanstalt für Landwirtschaft und Ernährung (BLE) Untersuchungen zum Einfluss unterschiedlicher Pflanzenschutzmaßnahmen auf den Mykotoxingehalt und den Fusarium-Besatz im Erntegut von Getreide (1997–2000). Ergänzt um Beiträge der Projektgruppenteilnehmer aus dem eigenen Forschungs- und Entwicklungsbereich wurden die Erkenntnisse der Projektgruppe zu diesem Themenkomplex im Rahmen eines Workshops im November 2002 vorgestellt und mit ca. 120 Fachleuten aus Ministerien, der Produktionstechnik sowie den vor- und nachgelagerten Bereichen diskutiert.Darüber hinaus wurden in verschiedenen Redaktionsgruppen Bekämpfungsschwellen harmonisiert (1996) und Umfrageergebnisse zu den Ursachen der Entwicklung einer Fungizidresistenz (2001) sowie zum regionalen Ausmaß unterschiedlicher Vorfrüchte und Bodenbearbeitungsverfahren zu Winterweizen im Zusammenhang mit dem Komplex Ährenfusariosen/Mykotoxine (2002) bearbeitet.Als weitere Schwerpunkte wurden Gaeumannomyces graminis, Saatzeitversuche in Winterweizen sowie nichtparasitäre und Ramularia-Blattflecken behandelt. Die Frage nach möglichen Auswirkungen der ab 01.01.2005 gültigen EU-Agrarreform, des Pflanzenschutzmittelreduktionsprogramms der Bundesregierung und des Klimawandels auf den Pflanzenschutz wird aufgeworfen.     

Erfahrungen bei der bek?mpfung der wanderratte,Rattus norvegicus (Berk.) 1999 in Magdeburg

Data of a pest control business was used to study infestation with the brown, or Norway rat (Rattus norvegicus Berkenhout, 1769]) in the City of Magdeburg for the year 1999. Swarms of rats were found to live all over the territory of the city. Blocks of residential buildings erected during G. D. R. times and, more specifically, their basements were noted to be focal sites of infestation with rats. The problem of rats living in the sewerage system of the city turned out to be unresolved. Detailed reference is made to rodenticides and active substances as well as to control strategies. The priority given to economical issues over the application of scientific findings is considered to be the decisive factor which accounts for the needs in the extermination of rats.      

Erfahrungen bei der bek?mpfung der wanderratte,Rattus norvegicus (Berk.) 1999 in Magdeburg

Data of a pest control business was used to study infestation with the brown, or Norway rat (Rattus norvegicus Berkenhout, 1769]) in the City of Magdeburg for the year 1999. Swarms of rats were found to live all over the territory of the city. Blocks of residential buildings erected during G. D. R. times and, more specifically, their basements were noted to be focal sites of infestation with rats. The problem of rats living in the sewerage system of the city turned out to be unresolved. Detailed reference is made to rodenticides and active substances as well as to control strategies. The priority given to economical issues over the application of scientific findings is considered to be the decisive factor which accounts for the needs in the extermination of rats.     

Wirkung verschiedener Fungizide auf den Befall der D��rrfleckenkrankheit (Alternaria spp.) sowie auf den Ertrag der Kartoffel

Potato early blight caused by Alternaria solani and Alternaria alternata is a major disease of potatoes and other Solanaceae. By now, Alternaria species have established themselves as destructive pathogens. Due to considerable yield losses early blight is getting a serious problem in many German potato growing areas. Up to now, the management of this disease is achieved mainly through protective fungicide applications. By this, fungicides with different mode of actions have a considerable influence on the course of disease progress. For this, field trials were carried out in 2007 and 2008 and the efficacy of various active ingredients was determined. Marked differences were observed in the control of early blight disease due to the effectiveness of applied active ingredients. The application of potent fungicides, which are highly efficient, resulted in prolonged photosynthesis. As a result of this potato yield increased significantly. Our investigation show, that disease control can be better achieved with strobilurines like Azoxystrobin or Boscalid + Pyraclostrobin than with Mancozeb, Chlorthalonil or Fenamidon.     

Untersuchungen zur Bekämpfung von Beifußblättriger Ambrosie (Ambrosia artemisiifolia L.) mit herbiziden Wirkstoffen

Measures for the control of A.??artemisiifolia have to be adjusted to allow for the plant density in the stands. In Brandenburg and Saxony numerous locations of A.??artemisiifolia with low density of the species have been reported. For those stands eradication arrangements are scheduled and also practicable. Furthermore there are, especially in Brandenburg, agricultural areas with a high density of A.??artemisiifolia. The goal in these locations is the control of further spread, combined with the reduction of allergean potential. Approaches to the control A.??artemisiifolia are demonstrated by testing of herbicides in different agricultural crops. High efficacy (up to 100%) was reached in maize using approved herbicides. The active substance imazamox showed the highest efficacy against A.??artemisiifolia in peas. Cultiviation of tribenuron-tolerant sunflower varieties and the use of tribenuron-methyl offers the opportunity to eliminate the competition of A.??artemisiifolia even in strongly infested areas. In grass land and public green space the active substance clopyralid controls A.??artemisiifolia well. Non-selective active substances like glyphosate and glufosinate are appropriate to control A.??artemisiifolia in non-crop areas. Organic farming shows a reduction of A.??artemisiifolia taking adequate agricultural measures. However further spread of A.??artemisiifolia cannot be prevented in organic farming.