Comparison of bacterial organisms and their susceptibility patterns from otic exudate and ear tissue from the vertical ear canal of dogs undergoing a total ear canal ablation

The purpose of this prospective study was to compare bacterial organisms and their susceptibility patterns from otic exudate and ear tissue from the vertical ear canal of six dogs with end-stage otitis undergoing a total ear canal ablation. Twenty-six organisms, 13 from the exudate and 13 from the tissue, were morphologically and biochemically similar, and their susceptibility patterns were compared. There were discrepancies for only 5 antibiotics for 4 organisms from 3 dogs. Culture of otic exudate from the vertical ear canal of dogs with chronic end-stage otitis externa provides an accurate reflection of the bacterial species present and the antimicrobial susceptibility patterns of these infections.     

从犬的剪耳、断尾说起

在犬展上、电视、电影里面你一定见到过,本应天生垂耳的狗狗的耳朵却向上方生长,有些狗的尾巴非常短,以致于会让我们认为这些狗是没有尾巴的。其实,这些狗都是从小做了剪耳、断尾手术的。所谓剪耳,就是在狗狗耳朵的基础上剪掉它们三分之二的包括耳廓的部分,以此来使天生垂耳的狗狗的耳朵能够向上方生长的手术;断尾是人们通过实施手术将狗的尾巴截断的手术。     

种公牛耳组织成纤维细胞的分离与培养

哺乳动物细胞培养始于20世纪50年代,发展至今已成为生物、医学研究和应用中广泛采用的技术方法.近年来,体细胞克隆技术得到迅速发展,不同细胞类型供体的克隆动物相继产生.     

遮穗和去颖下燕麦穗部特征变化和穗部光合贡献率估算

随着探索提高植物整体光合能力相关研究的不断开展，麦类作物穗部器官等植物非叶绿色器官光合潜力挖掘逐渐得到关注。本研究在成都平原秋播美达、贝勒、莫尼卡、摄政王、泰克和甜燕60等6个品种燕麦，设置遮穗、去颖2个试验处理，比较分析了各品种间穗部特征、穗部光合贡献率、颖片光合贡献率和茎光合物质转移率等差异。结果表明，燕麦穗部器官光合贡献率为28.56%～49.05%，其中甜燕60最高；6个品种燕麦的颖片光合贡献率为11.03%～36.88%，茎光合物质转移率为6.65%～35.81%。燕麦穗部器官对籽实增重表现了较高的光合贡献，当燕麦穗部器官光合受到限制时，燕麦单粒种子重和单穗种子数显著降低，尤其是影响双粒小穗数。     

陆川猪耳部成纤维细胞的体外培养

本研究建立陆川猪耳部成纤维细胞的体外培养体系,采用组织块培养法可以获得陆川猪耳部成纤维细胞。用0.25%胰蛋白酶＋0.02%EDTA消化液消化细胞、用含有10%FBS的DMEM对细胞进行培养,能很好的支持陆川猪耳部成纤维细胞的生长。传3代后,观察到培养的细胞形态逐渐均一,为典型的成纤维细胞,绝大部分呈梭形或不规则三角形...     

牛耳静脉采血方法简析

牛耳静脉采血具有操作简便,全血采集质量好,采血速度快,对牛只应激小,耗材成本低等优点。本文主要从采血用品、采血部位、操作方法和注意事项等方面,简要介绍牛耳静脉采血方法。     

Births of freemartins derived from embryos reconstructed with ear fibroblasts.

Although the combination of artificial insemination (AI) and embryo transfer (ET) is effective for preventing large offspring syndrome in clone cattle production, it may cause freemartinism. In this study, 51 reconstructed embryos were transferred to artificially inseminated recipients. Of those 9 twin pregnancies, three delivered male and female offsprings. The females had tufts of long coarse hair and short blind pouch at the vaginal end. At necropsy, hypoplastic testicles and epididymis, which connected to the uterus through the spermatic cord, were found and seminal vesicles were also noted. All females had mixed sex chromosome configuration (60, XX and 60, XY). These results suggest that the combined ET program can cause freemartinism, which reduces the efficiency of clone cattle production.     

The occurrence and anti-microbial susceptibility of Salmonellae isolated from commercially available pig ear pet treats

In Canada, there have been reported outbreaks of human salmonellosis related to exposure to animal-derived pet treats, involving pig ear treats, beef steak patty dog treats and pet treats of seafood origin. As a follow-up to recommendations made to the pig ear treat industry in 1999, a total of 245 pig ear treats were purchased in two Canadian cities to provide evidence of adoption of the recommendations and to determine the current levels of Salmonella contamination of pig ear treats available at pet stores. An overall prevalence of 4% was observed, with isolates exhibiting resistance to up to seven anti-microbials. Serotypes recovered included S. Bovismorbificans, S. Give, S. Derby and S. Typhimurium var. Copenhagen. Although the prevalence observed during this study is lower than the prevalence observed in 1999, pig ear treats should still be considered as a possible source of Salmonella and anti-microbial resistant bacteria to humans and dogs in Canada.     

Surgery of the ear and pinna.

There are several disease processes of the ear and pinna that warrant surgical intervention. This article reviews surgical anatomy and common surgical procedures of the ear and pinna, including aural hematomas, lateral wall resection, vertical ear canal resection, total ear canal ablation and lateral bulla osteotomy, partial pinna resection, and feline inflammatory polyps. The clinical signs, diagnosis, and surgical treatment along with potential complications for each disease process are discussed.     

Comparison of haematological analyses of blood taken from the cephalic and marginal ear veins in cats

Various haematological components were compared in blood samples from the cephalic and marginal ear vein of 21 cats. Test results tended to be higher in blood samples from the cephalic vein, although significant differences were only detected for haemoglobin concentration, white blood cell count and the absolute numbers of neutrophilic granulocytes and lymphocytes. Differences in test results between the two veins were small and apparently of only minor practical importance, thereby further substantiating the suitability of the marginal ear vein nick technique as a means of obtaining small blood samples from cats.     

Canine ear cropping

Extract

Sir:- At a recent meeting, the members of the Small Animal Society of the N.Z.V.A. voted to state their unanimous objection to the practice of cropping canine ears for cosmetic reasons.     

In vitro antimicrobial activity of orbifloxacin against Staphylococcus intermedius isolates from canine skin and ear infections

The objective of the study was to evaluate the in vitro activity of orbifloxacin against Staphylococcus intermedius strains isolated in France from canine skin and ear infections. The minimum inhibitory concentrations (MICs) of orbifloxacin against 240 field S. intermedius isolates (69 skin and 171 ear isolates) ranged from 0.016 to 8 mg l(-1), with MIC50 and MIC90 equal to 0.5 and 1 mg l(-1), respectively. Only one strain, a pyoderma isolate was resistant (MIC=8 mg l(-1)). Orbifloxacin was tested at different concentrations for killing rate against five isolates obtained from pyoderma cases and against a reference strain (Staphylococcus aureus ATCC 29213). Orbifloxacin expressed a concentration-dependent bactericidal activity against the S. aureus reference strain, but a time-dependent bactericidal activity against S. intermedius. Orbifloxacin induced bactericidal effect against the S. intermedius strains tested with concentrations equal to or two times MIC.     

A semiquantitative cytological evaluation of normal and pathological samples from the external ear canal of dogs and cats

Abstract  Numbers of desquamated epithelial cells, yeast cells and bacterial organisms were counted in samples collected from the external ear canal of 37 normal dogs and 16 normal cats, and from 24 dogs and 22 cats with otitis externa. The aims of the study were to establish quantitative reference ranges and to correlate these data with the clinical status of the dogs and cats. Numbers of yeast cells and bacterial organisms were significantly increased in dogs ( P  = 0.05; P  = 0.0001) and cats ( P  = 0.0001; P  = 0.0001) with otitis externa, and in most cases high counts were correlated with clinical signs. Mean Malassezia counts per high-power dry field of ≥ 5 in the dog and ≥ 12 in the cat were considered abnormal. Mean bacterial counts per high-power dry field of ≥ 25 in the dog and ≥ 15 in the cat were considered abnormal. When used to differentiate normal from inflamed external ear canals, these figures provided a low sensitivity but a specificity of ≥ 95%.