共查询到20条相似文献，搜索用时 30 毫秒
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Jensen AL Iversen L Høier R 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》1999,28(2):53-56
The aim of the present study was to evaluate analytical performance using total error criteria of a commercial enzyme immunometric assay for the determination of endogenous canine thyrotropin (TSH). The allowable total error for this assay (22.6%) was estimated using previously reported data on biological variation. Inaccuracy and imprecision of the assay (0% and 5.7% for the low control material; 6.8% and 3.0% for the high control material) were estimated by measuring the same lot of control material for 21 consecutive weeks, during which time the assay was considered stable and in control. Analytical performance was assessed using a MEDx chart, a graphical tool for comparing inaccuracy and imprecision, with an analytical quality requirement stated in the form of allowable total error. The results of the present study showed that the canine TSH assay had good to excellent analytical performance. 相似文献
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Reasons for performing study: Accurate measurement of plasma fibrinogen concentrations is an important tool for assessment of horses with inflammatory diseases. Objectives: To determine the precision and accuracy of a benchtop instrument using both fresh and frozen equine plasma by comparing the plasma fibrinogen concentration measured by a benchtop instrument to 2 separate laboratory standard methods (ACL 100 and STA Compact) for fibrinogen measurement. Methods: Accuracy and precision of the VSPro was evaluated using both human fibrinogen standards and samples from horses. Fifty frozen samples from horses with gastrointestinal disease had the fibrinogen concentration measured using the ACL 1000 and the VSPro. Fifty fresh samples were collected from hospitalised horses and fibrinogen concentration was measured using the STA Compact coagulation machine and the VSPro. Correlations for measurements were performed, as well as Bland‐Altman analysis. Results: Coefficients of variability for the VSPro ranged from 7% to 15%. The VSPro fibrinogen values were well correlated to both the ACL 1000 (r = 0.94, P<0.001) and the STA Compact measurements (r = 0.926, P<0.001). Bland‐Altman analysis showed a mean bias of ‐0.83 g/l (95% confidence interval ‐2.03–0.324 g/l) for the ACL 1000 and a mean bias of ‐0.024 g/l (95% confidence interval ‐1.434–1.386 g/l) for the STA Compact. Conclusions: The VSPro appears to have adequate accuracy and precision for clinical measurement of plasma fibrinogen concentrations. Potential relevance: The VSPro provides a measurement of equine plasma fibrinogen concentration using a benchtop instrument with a rapid test time that has comparable accuracy to the fibrinogen concentration obtained from reference laboratories. 相似文献
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Shiel RE Brennan SF O'Rourke LG McCullough M Mooney CT 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2007,36(3):274-277
BACKGROUND: Greyhound dogs have numerous clinicopathologic differences compared with other dog breeds, most notably in their hematologic profiles. The hematologic differences are likely related to breed; however, the influence of other factors, including age, sex, and training, has not been fully determined. OBJECTIVES: The aim of this study was to assess hematologic values in young, healthy, pretraining Greyhounds and to determine the effects of age and sex on these findings. METHODS: Jugular venous EDTA-anticoagulated blood samples were collected from 43 healthy, pretraining Greyhounds between 5 and 13 months of age. Samples were analyzed within 24 hours of collection on an Abbott CELL-DYN 3500R hematology analyzer. Mean hematologic results for different age groups, and correlation with age and sex were determined for each analyte. Results were compared with adult canine, nonbreed-specific reference intervals. RESULTS: From the age of 9 to 10 months, Greyhounds had higher HCT, hemoglobin concentration, and RBC counts compared with adult canine reference intervals. Younger Greyhounds (5-6 months) had values comparable with reference intervals. Mean total WBC, neutrophil, lymphocyte, and platelet counts tended to be toward the lower end or below the reference intervals. HCT, hemoglobin concentration, and RBC counts were correlated positively with age, and platelet count was correlated negatively with age. No differences were found based on sex. CONCLUSIONS: These results confirm that significant hematologic differences exist in pretraining Greyhounds by 9 to 10 months of age, when compared with adult canine, nonbreed-specific reference intervals; however, these differences are less marked in Greyhounds 5 to 6 months old. Given these findings, it is unlikely that factors such as training or racing are responsible for differences in hematologic values between adult Greyhounds and other breeds. 相似文献
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Three techniques to measure utilization of karoo bushes were evaluated. Precision, accuracy and consistency were measured on three species at different times and levels of utilization. The percentage‐cover technique was less sensitive and less efficient than the percentage‐edible‐strikes and percentage‐twigs‐grazed techniques. It required samples that were 25% larger than those needed for the other two techniques to achieve equivalent levels of precision. For any single technique, precision was consistent for different species. Provided samples exceeded 30 bushes all techniques were consistent over time. No improvement in accuracy was measured for samples greater than 20 bushes. While similar levels of accuracy were achieved on the three species, the lack of consistency of the percentage‐cover technique for different levels of utilization, limits the usefulness of the technique. To estimate utilization of a single species using any of the techniques with adequate precision and accuracy, approximately 25 minutes with two operators was required. 相似文献
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E. Yildirim T. Karapinar A. Hayirli 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2015,29(1):388-394
Background
Rapid determination of blood electrolyte concentrations can help determine electrolyte status and delivery of effective volume of electrolyte solutions in field conditions.Objective
To evaluate reliability of the i‐STAT, a point‐of‐care (POC) device, in measuring blood K+, Na+, and CI− concentrations in cattle.Animals
Ninety‐eight cattle with various diseases.Methods
In this prospective study, blood samples collected from the jugular vein were processed for determination of K+, Na+, and CI− concentrations in blood and plasma using the i‐STAT and auto‐analyzer (Cobas C501), respectively. Blood and plasma electrolyte data were subjected to student t‐test for comparison, the concordance analysis for agreement, accuracy, and precision, the Passing‐Bablok regression and the Bland‐Altman plot for reliability, and receiver operating characteristics curves for sensitivity (Se) and specificity (Sp).Results
Plasma concentrations of K+ (4.39 versus 4.2 mmol/L; P < .0001) and CI− (100.30 versus 99.4 mmol/L; P < .04) were greater than their concentrations in blood. Plasma and blood Na+ concentrations were similar (136.95 versus 136.8 mmol/L). The i‐STAT results were highly correlated with the Cobas C501 results (r = 0.970, 0.922, and 0.866 for K+, Na+, and CI−, respectively). Regression equations fitting blood (Y) and plasma (X) concentration did not deviate from the identity line for K+ (Y = −0.10 + 0.98 × X), Na+ (Y = X), and CI− (Y = 3.04 + 0.96 × X). The mean bias (blood concentration ‐ plasma concentration) was −0.20 for K+ (P = .03), −0.16 for Na+ (P = .12), and −0.87 for CI− (P = .93). The i‐STAT had 76–100% Se and 87.7–100% Sp for assessing electrolyte statuses.Conclusions and Clinical Importance
The i‐STAT yielded results that were in agreement with the auto‐analyzer, with negligible biases in measurement of plasma K+, Na+, and CI− concentrations. The i‐STAT is a reliable POC device and can be used in field condition to assess electrolyte status in cattle. 相似文献15.
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Camilla A. Jamieson BVM & BVS Andrew S. Hanzlicek DVM MS DACVIM Mark E. Payton PhD Todd C. Holbrook DVM DACVIM DACVSMR 《Journal of Veterinary Emergency and Critical Care》2018,28(1):39-44