Colorimetric sensor array for soft drink analysis

Fourteen commercial soft drinks have been analyzed using colorimetric sensor arrays made from a set of 25 chemically responsive dyes printed on a hydrophobic membrane. Digital imaging of the dye array before and after immersion provides a color change profile as a unique fingerprint for each specific analyte. The digital data library generated was analyzed with statistical and chemometric methods, including principal component analysis (PCA) and hierarchical clustering analysis (HCA). Facile identification of all of the soft drinks was readily achieved using comparison of the color change profiles or a PCA score plot. Using a HCA dendrogram, the misclassification rate was <2%, and even very similar sodas were easily differentiated. In addition, the monitoring of soft drinks as they degas or upon dilution also proved to be possible. This work demonstrates the potential of our colorimetric sensor array technology for quality assurance/control applications of sodas and perhaps other beverages as well.     

Six-week consumption of a wild blueberry powder drink increases bifidobacteria in the human gut

Wild blueberries are a rich source of polyphenols and other compounds that are highly metabolized by the intestinal microbiota and may, at the same time, affect the intestinal environment itself. A repeated-measure, crossover dietary intervention on human volunteers was designed to study the effect of six week consumption of a wild blueberry ( Vaccinium angustifolium ) drink, versus a placebo drink, in modulating the intestinal microbiota. Relative to total eubacteria, Bifidobacterium spp. significantly increased following blueberry treatment (P ≤ 0.05), while Lactobacillus acidophilus increased after both treatments (P ≤ 0.05). No significant differences were observed for Bacteroides spp., Prevotella spp., Enterococcus spp., and Clostridium coccoides . Bifidobacteria, which have been largely proposed to be of benefit for the host, appeared to be selectively favored suggesting an important role for the polyphenols and fiber present in wild blueberries. Results obtained suggest that regular consumption of a wild blueberry drink can positively modulate the composition of the intestinal microbiota.     

轻简柔性墙体装配式日光温室能耗分析

轻简装配式日光温室的柔性墙体一般不具备蓄热能力,为评估其地区适应性,该研究以太阳辐射为主要指标,结合气候条件和日光温室发展选择中国17个城市,利用热平衡方程和日光温室热环境模拟软件RGWS-RHJPJ V1.2,计算最冷月室外最低温度下温室的采暖热负荷和冬至日的采暖需求量,并以主动蓄放热系统供热为例,分析轻简装配式日光温室太阳能热利用效果。研究结果表明：在所选城市中,轻简装配式日光温室采暖热负荷的范围为50～150 W/m2,随着城市地理纬度的升高,采暖热负荷增大,以冬至日为例,约20%城市轻简装配式日光温室的采暖需求量在0～2 MJ/(m2·d),约50%在2～4 MJ/(m2·d),仅10%大于6 MJ/(m2·d);对轻简装配式日光温室采暖热负荷影响最大的参数是南屋面保温被传热系数,其次是温室的换气次数;在拉萨和昌都,主动蓄放热系统对轻简装配式日光温室采暖需求量的满足率为100%。轻简装配式日光温室的采暖热负荷和采暖需求量在中国不同气候区差异较大,且主动蓄放热系统的加温效果在地区间差异也很大。研究结果对中国轻简装配式日光温室的建造、供暖设计具有一定的参考价值。     

Determinants of urban bushmeat consumption in Río Muni, Equatorial Guinea

Understanding the factors driving demand for wild meat and its substitutes is crucial for predicting the effects of changing socio-economic conditions on consumption, and managing supplies sustainably. However urban demand for wild meat remains relatively understudied, particularly in West/Central Africa. We use interviews with consumers in households, markets and restaurants and a market survey to examine patterns of consumption of bushmeat, domestic meat and fish in Bata, Equatorial Guinea, a country currently undergoing a period of strong economic and population growth. Consumers make a much clearer distinction between fresh and frozen foods than between bushmeat, domestic meat and fish. Fresh foods are greatly preferred over frozen but are more expensive and less consumed. Consumption of all fresh foods increases with income. Controlling for income, native Equatoguineans consume more bushmeat than other nationalities, while of the two dominant Equatoguinean tribes, the continental Fang consume more bushmeat than the coastal Ndowe. Our findings indicate that increasing wealth of a growing urban population will greatly increase future demand for all fresh foods, including bushmeat. There is no evidence of a luxury bushmeat market based on rare species, thus controlled demand for bushmeat could be met from common, highly productive species that are relatively robust to exploitation. Improving the supply of under-developed commodities, particularly domestic livestock, could also offset demand for bushmeat.     

The stability of soft drinks intake from adolescence to adult age and the association between long-term consumption of soft drinks and lifestyle factors and body weight

OBJECTIVES: To investigate the tracking of sugar-sweetened, carbonated soft drinks intake from age 15 to 33 years and the association between this intake and lifestyle factors and body weight. DESIGN: A longitudinal study with 18-20 years of follow-up. Data about diet, physical activity, smoking and dieting were collected in 1981/1979, 1991 and 1999. Body weight and height were measured in 1981/1979 and self-reported in 1999. SETTING: Oslo, Norway. SUBJECTS: Four hundred and twenty-two men and women. RESULTS: Tracking of soft drinks intake from adolescence into early adulthood (age 25 years) and from early adulthood into later adulthood (33 years) was moderate to high, while tracking from adolescence into later adulthood was low. Comparing those reporting a high intake of soft drinks in both 1991 and 1999 with those reporting a low intake at both times, male long-term high consumers were more likely to smoke (48 vs. 21%, P=0.002) and reported higher intakes of energy (12.2 vs. 10.2 MJ day(-1), P=0.005) and sugar (142 vs. 50 g day(-1), P<0.001) in 1999 than did long-term low consumers. Women high consumers were less likely to be physically active (14 vs. 42%, P=0.03) and had higher sugar intake (87 vs. 41 g day(-1), P<0.001) in 1999 than did women low consumers. There were no differences in body mass index, overweight or obesity in 1999 between long-term high and low consumers. CONCLUSION: In this study, stability of soft drinks intake from age 15 to 25 years and from age 25 to 33 years was moderate to high, while from age 15 to 33 years it was low. Soft drinks intake from age 25 to 33 years was associated with smoking and physical inactivity, but not with body weight.     

A pilot study on the effect of short-term consumption of a polyphenol rich drink on biomarkers of coronary artery disease defined by urinary proteomics

Polyphenol rich diets have been associated with a reduced risk of cardiovascular disease. We examined the effect of a polyphenol rich (P-R) drink on biomarkers assessed by urinary proteomics. Thirty nine middle aged and overweight subjects were randomized to P-R drink (n = 20) or placebo (n = 19) in addition to their normal diet. After two weeks urine samples were obtained for assessment of the urinary proteome using capillary electrophoresis coupled to a mass spectrometer. A total of 93 polypeptides were found to be candidates for differential distribution with a nominal p-value <0.05, though these differences did not reach significance when multiple testing was accounted for. Sequences were determined in 19 of these demonstrating that they originate from alpha-1 antitrypsin, collagens, fibrinogen alpha and IgG kappa. Levels of 27 polypeptides were greater than 4-fold different between the two groups. Of these, 7 were previously found to be part of a coronary artery disease (CAD) specific urinary biomarker pattern. Their direction of expression was closer to the healthy state in the P-R drink group and closer to CAD state in the placebo group. Our data suggest that the P-R drink may have beneficial effects on urinary biomarkers of CAD. The data encourage the planning of future prospective studies, aimed at investigating significant effects of polyphenol rich dietary products.     

毛樱桃汁碳酸饮料生产工艺研究

分析了毛樱桃的营养成分,研究了果胶酶ROHAPECT D5S对毛樱桃的出汁率、粘度、澄清度的影响。结果表明,毛樱桃营养丰富,矿物质和维生素等含量较高;复合果胶酶处理对出汁率的影响显著,酶的浓度为6%、处理温度为45℃、处理时间为60 min时出汁率最高,达到72.8%,提高了11.6%;并且酶的浓度能极大地降低果汁的粘度,使果汁澄清度加大,差异显著;同时优选出毛樱桃汁碳酸饮料的最佳配方。     

枇杷叶植物饮料悬浮稳定性和口味的优化

引用流变学原理改善枇杷叶植物饮料悬浮稳定性,以正交试验方案优化了辅料配方,制成口味优良的饮料。结果表明：澄清的枇杷叶水提液是一个复杂的多分散相系统,所含粒子颗粒(固相)大小、分散相介质(液相)粘度以及固液两相间的密度差是影响悬浮稳定性的主要因素,依Stokes定律进行改良可有效提高稳定性。枇杷叶植物饮料的口味配方为澄清的枇杷叶水提液用量70%,蔗糖6%,蜂蜜2.4%,柠檬酸0.4%,复合稠化剂0.15%,产品中含黄酮类化合物0.858～0.987 mg/L,氨基酸108～115 mg/L,可溶性糖210～230 mg/L。     

The impact of knowledge and social influences on adolescents' breast-feeding beliefs and intentions

OBJECTIVES: Many health promotion educational interventions assume that increasing knowledge directly influences beliefs, intentions and behaviour, whereas research suggests that knowledge alone is insufficient for behavioural change. Social cognition frameworks such as the Theory of Reasoned Action propose a central role for beliefs and social normative influences. This Scottish study evaluates the role of knowledge and social influences (subjective norms, exposure to breast-feeding, social barriers) on beliefs and future intentions to breast-feed or bottle-feed. Social influences from family and peers are investigated.DESIGN: A cross-sectional between-subjects observational design was used. A questionnaire was administered to a sample of 229 (46%) male and 267 (54%) female adolescents aged 11-18 years.SETTING: Participants completed questionnaires during lessons at three secondary schools in Central Scotland.RESULTS: Knowledge about health benefits of breast-feeding was generally poor. Analyses found that perceived social barriers to breast-feeding moderated the relationship between knowledge and beliefs. More knowledge, positive beliefs and supportive subjective norms also predicted future intentions to breast-feed. Parental norms exerted greater influence than peer norms on adolescents' breast-feeding beliefs.CONCLUSIONS: Knowledge and social influences are important predictors of positive breast-feeding beliefs and future intentions to breast-feed in adolescents. This has important implications for breast-feeding health promotion interventions in young people.     

Changes in the aroma of a strawberry drink during storage.

The flavor of a commercially available strawberry drink was investigated with special regard to the changes of the sensory properties during the shelf life of the product. The experiments were performed using gas chromatographic methods after liquid-liquid extraction and after solid-phase microextraction of the headspace. A trained sensory test panel was used to substantiate the results from instrumental analyses. The relative concentrations of several compounds were followed over a storage period of six weeks at elevated temperature (37 degrees C), which corresponds to about 12 months storage at room temperature. Significant concentration changes of several flavor compounds were determined after a short storage time. These results correlate highly with changes in the aroma observed by the sensory test panel. Further on, changes in the sensorial relevance of aroma active compounds were monitored by comparative aroma extract dilution analysis of extracts of the fresh product and the product at the end of the declared shelf-life time. The results showed a significant decrease in flavor dilution factors of compounds with characteristic fruity attributes.     

Change in the flavor of black tea drink during heat processing.

Heat processing during canning is responsible for the change in flavor of black tea infusion. The quantitative change in the volatile components of the black tea infusion during heat processing is not sufficient for explaining the sensory evaluation. In this study, application of aroma extract dilution analysis using the volatile fraction before and after black tea (Darjeeling) samples were heat processed resulted in the detection of 10 odor-active peaks for which flavor dilution (FD) factors changed. Seven potent odorants were identified from these peaks by gas chromatography-mass spectrometry. Among these components, 3-methylbutanal (stimulus), methional (potato-like), beta-damascenone (sweet), dimethyl trisulfide (putrid), and 2-methoxy-4-vinylphenol (clove-like) showed the highest FD factors after heat processing of the black tea sample. Therefore, these odorants were the most important components involved in changing the black tea odor during heat processing. In addition, the precursor of beta-damascenone in black tea infusion was investigated, and 3-hydroxy-7,8-didehydro-beta-ionol was determined to be one of the beta-damascenone-generating compounds for the first time.     

Investigation of the change in the flavor of a coffee drink during heat processing

Heat processing is responsible for the change in the flavor of a coffee drink. In this study, the application of gas chromatography-olfactometry of headspace samples (GCO-H) using the vapor fraction before and after heat processing of the coffee samples resulted in the detection of 12 odor-active peaks for which the flavor dilution (FD) factors changed. Eight potent odorants were identified from these peaks by gas chromatography-mass spectrometry (GC-MS). Among these components, methanethiol (putrid), acetic acid (sour), 3-methylbutanoic acid (sour), 2-furfuryl methyl disulfide (meaty), and 4-hydroxy-2,5-dimethyl-3(2H)-furanone (caramel-like) increased after heating of the coffee sample, whereas 2-furfurylthiol (roasty), methional (potato-like), and 3-mercapto-3-methylbutyl formate (roasty) decreased compared with the coffee sample before heat treatment. In addition, extensive studies have been carried out on the pH effects on the change in the concentration of 2-furfurylthiol during heat processing and in the pH range of 5-7; it was found that the concentration of this compound in the model solutions had significantly changed.     

Seed consumption by millipedes

Seed predation is a key mortality factor for plants that reproduce by seeds. We investigated seed consumption by Cylindroiulus caeruleocinctus (Wood, 1864), an abundant representative of an unrecognised group of seed predators – the millipedes (Diplopoda: Julidae). The consumption of small seed species by millipedes was measured in the absence (41 species) and in the presence (11 species) of alternative food sources (dead leaves). We examined whether millipedes would consume seeds at all and if so whether there were any seed size constraints that determined millipede seed acceptance (i.e., the proportion of millipedes that ate seeds) and seed consumption (i.e., the mass of the seeds eaten). Our results indicated that C. caeruleocinctus readily accepted seeds with 75% of seed species accepted in a no-choice experiment and 100% of seed species in a choice experiment. Seed acceptance decreased with increasing seed mass but increased with increasing millipede mass. Seed consumption increased with the size of the offered seed species but was found to be independent of millipede mass. After the millipedes were given dead leaves to consume, the acceptance slightly decreased, and the consumption of the more preferred seed species decreased. Seeds form a small part of the millipede diet but are consumed regularly. Because of millipedes’ foraging preferences and their patchy distribution they may notably contribute to the local mortality of some seed species in their natural environment.     

Seed consumption by millipedes

Seed predation is a key mortality factor for plants that reproduce by seeds. We investigated seed consumption by Cylindroiulus caeruleocinctus (Wood, 1864), an abundant representative of an unrecognised group of seed predators - the millipedes (Diplopoda: Julidae). The consumption of small seed species by millipedes was measured in the absence (41 species) and in the presence (11 species) of alternative food sources (dead leaves). We examined whether millipedes would consume seeds at all and if so whether there were any seed size constraints that determined millipede seed acceptance (i.e., the proportion of millipedes that ate seeds) and seed consumption (i.e., the mass of the seeds eaten). Our results indicated that C. caeruleocinctus readily accepted seeds with 75% of seed species accepted in a no-choice experiment and 100% of seed species in a choice experiment. Seed acceptance decreased with increasing seed mass but increased with increasing millipede mass. Seed consumption increased with the size of the offered seed species but was found to be independent of millipede mass. After the millipedes were given dead leaves to consume, the acceptance slightly decreased, and the consumption of the more preferred seed species decreased. Seeds form a small part of the millipede diet but are consumed regularly. Because of millipedes’ foraging preferences and their patchy distribution they may notably contribute to the local mortality of some seed species in their natural environment.     

Effect of a tomato-based drink on markers of inflammation, immunomodulation, and oxidative stress

Regular consumption of tomato and its products is being consistently associated with lower risk of several types of cancer and, to a lesser extent, coronary heart disease. Among the many tomato components credited with healthful properties, carotenoids and particularly lycopene are being actively investigated. Given the recognized role of immune/inflammatory processes in atherogenesis, the effects of a tomato-based drink (Lyc-o-Mato), which was previously shown to afford DNA protection from oxidative stress, on the modulation of immune and inflammatory markers (by enzyme immunoessay), on basal lymphocyte DNA damage (by comet assay), and on F2-isoprostane excretion (by LC-MS/MS), were investigated in 26 healthy young volunteers. In a placebo-controlled, double-blind, crossover study, Lyc-o-Mato (5.7 mg of lycopene, 3.7 mg of phytoene, 2.7 mg of phytofluene, 1 mg of beta-carotene, and 1.8 mg of alpha-tocopherol) or a placebo drink (same taste and flavor, but devoid of active compounds) were given for 26 days, separated by a wash-out period. During the study subjects maintained their habitual, hence unrestricted, diet. TNF-alpha production by whole blood was 34.4% lower after 26 days of drink consumption, whereas the other parameters were not significantly modified by the treatment. In turn, modest effects of the regular intake of a tomato drink, providing small amounts of carotenoids, were found on the production of inflammatory mediators, such as TNF-alpha, in young healthy volunteers. Future intervention trials in subjects with low carotenoid status and/or compromised immune system will resolve the issue of whether carotenoids modulate immune parameters in humans.