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1.
Aphid colonies were established on one second-node leaflet of young broad bean plants and sublethal treatments of 14C-labelled phorate were applied to the adjacent leaflets. After 3 days, the amounts of toxic and non-toxic 14C-labelled compounds in the aphids, their honeydew and the untreated foliage were determined. There were no significant differences between the amounts in leaves at the same level on the plant when infested and aphid-free plants were compared, but the aphids and their honey-dew contained two and 23 times as much of the toxic and non-toxic 14C-compounds, respectively, found in the host leaflets. Following translocation to aphids on leaves above or below treated leaflets, the aphid colonies again contained more labelled compounds than the host-plant leaves. The movement of non-toxic compounds into the roots was reduced when the aphid colonies were situated on foliage between the site of treatment and the roots. More of the toxic and the non-toxic fractions were translocated downwards from the third to the second leaf than in the reverse direction.  相似文献   

2.
西瓜花叶病毒2号(WMV-2)对棉蚜生物学特性的影响   总被引:1,自引:0,他引:1  
通过对棉蚜寄生选择、显微测量、解剖法及室内培养,研究了西瓜花叶病毒2号对棉蚜寄生甜瓜的趋向性、体长、胚胎数量、繁殖力等的影响。结果表明,棉蚜嗜好感病的甜瓜;病株上棉蚜胚胎数目比健株多2~3个;病株上棉蚜的体长大于健康株;用Logistic曲线拟合,病株上棉蚜最大生物容纳量和瞬时增加速率大于健株;在寄主甜瓜接毒2周内,病株上棉蚜个体繁殖力大于健株,但接毒2周后,病株上棉蚜的繁殖力小于健株;病毒WMV-2对棉蚜种群结构也有影响。  相似文献   

3.
柑橘衰退病毒含量对其蚜传效率的影响   总被引:1,自引:0,他引:1  
为明确毒源植株和蚜虫中柑橘衰退病毒(Citrus tristeza virus,CTV)的发生情况与蚜虫传播CTV能力的关系,将褐色橘蚜、棉蚜、橘二叉蚜和绣线菊蚜置于分别感染了4个CTV分离株的锦橙上取食24 h后,运用巢式RT-PCR和实时RT-PCR检测蚜虫和锦橙的带毒情况,并分析蚜虫的传毒能力。结果显示,蚜虫中CTV的平均带毒率为0.76~0.84,其中棉蚜的最高,其次为绣线菊蚜、褐色橘蚜和橘二叉蚜。锦橙中各CTV分离株的含量差异不大,与蚜虫传毒效率间无显著相关性;也未发现蚜虫带毒率与其传播CTV能力之间存在相关性。蚜虫体内CTV的含量为5.36×103±2.33×103~2.01×106±3.67×105拷贝,褐色橘蚜中含量最高,其次为棉蚜、橘二叉蚜和绣线菊蚜;且高蚜传能力CTV分离株在褐色橘蚜体内的含量远高于低蚜传能力分离株。表明蚜虫体内CTV的含量可能与蚜虫传毒能力有关。  相似文献   

4.

The resistance of ashgourd to the melon-cottonaphid, Aphis gossypii (Glover), and the incidence of aphid-transmitted viral diseases was investigated following recording of aphid numbers and trichome (plant hair) numbers per cm2 of leaf on four parts of the cucurbit, ashgourd, representing four genotypes (Local Sylhet, Local Round, High Female and CQ-10-90). The Local Sylhet genotype was found to have the highest trichome density compared with those of Local Round, High Female, and CQ-10-90 ashgourd genotypes, and was least infested by Aphis gossypii. The percentage of virus-infected plants was also found to be lowest on the Local Sylhet genotype. Aphid numbers were lowest on the terminal and young leaves of ashgourd compared with mature and senescent leaves, due to the higher trichome density in the former leaf categories. Trichome density was found to have a significant negative influence on the number of aphids. Trichomes in ashgourd play a major role by providing a first line defence in reducing aphid infestation, with a subsequent beneficial effect of reducing the incidence of virus.  相似文献   

5.
Aphid feeding in cereals results in both quantitative and qualitative yield losses; moreover aphid-transmitted viruses are responsible for other quantitative and qualitative damage so that direct or indirect effects of aphid infection are always of interest. At the beginning of stem elongation, wheat plants were caged regardless of the presence or absence of indigenous cereal aphids. Half of the caged plants were artificially infected with Diuraphis noxia. The number of D noxia and Rhopalosiphum padi individuals on plants destined for flour preparation was approximately 150 aphids per tiller at peak population. The number of Sitobion avenae individuals was ca 25 aphids per tiller. Bread-making quality of wheat flour is determined primarily by the protein content, the gluten proteins (gliadins, glutenins) being the prime factors. The absolute amounts and/or the relative proportion of gliadins and glutenins are very important in dough making and in determining baking quality. Flour from grains originating from plants infected naturally with cereal aphids indigenous to Hungary, and flour infected artificially with D noxia, were analysed for glutenin and gliadin content using size exclusion HPLC. It was found that aphid infection had a significant effect on the gliadin/glutenin ratio, which was significantly lower in wheat flour prepared from aphid-infected plants than in that from uninfected control. The most significant decrease in gliadin/glutenin ratio was caused by D noxia infection, followed by R padi and then S avenae. As the gliadin/glutenin ratio was significantly lower in flour made from aphid-infected wheat seeds, it can be supposed that aphid feeding results in decreased bread making quality of wheat flour.  相似文献   

6.
The species composition and abundance of aphids in commercial cv. Agate and cv. Super Pride hop gardens in Tasmania, Australia, were characterized over three seasons (1999–2001). Gunns Plains recorded 14 aphid species and Bushy Park 11 species, with nine of these common to both sites. The majority of aphids were trapped in the first 2 months (October and November) of active hop growth in all three seasons. Cultivar and geographical location had significant effects on the abundance of total aphids (species pooled) trapped and several individual aphid species in the three seasons. In general, significantly more aphids (total and individual species) were trapped in cv. Agate than cv. Super Pride gardens, and higher numbers were trapped at Bushy Park than at Gunns Plains. This coincided with a higher incidence of plants infected by carlaviruses in cv. Agate gardens at both locations. Differences in the spatiotemporal dynamics of Carlavirus epidemics were described by fitting a stochastic model to the data, with parameters for local spread within the garden (contagion) and background infection (disease increase unrelated to infected plants within the gardens). Local spread of Hop latent virus (HpLV) and Hop mosaic virus (HpMV) was indicated within all gardens. For HpMV in cv. Agate at Gunns Plains, however, infections caused by immigrant viruliferous aphids were also apparent. Using join-count statistics, spatial aggregation of both virus diseases was found for all years, except for the initial year (1999) when incidence was low. Clusters of diseased plants extended to greater distances for HpLV than for HpMV. Based on spatial and spatiotemporal analyses, local spread (mechanical transmission and/or aphid movement within the garden) appears to be the dominant factor in the epidemics of HpLV. Aphid immigration from outside the crop over time may play a more significant role for HpMV epidemics, at least for one location.  相似文献   

7.
植物抗蚜生理及分子机制的研究进展   总被引:1,自引:0,他引:1  
蚜虫作为农业害虫和植物病毒的传播者,已经成为严重威胁全球农业生产发展的主要害虫之一。本文综述了植物宿主免疫系统在抗蚜过程中的作用,从物理屏障、化感元素以及基因调控等方面阐述目前的抗蚜应答机理研究,提出了抗蚜研究应该整体地考虑植物-蚜虫互作动态体系,抗蚜基因的挖掘应该建立在有效性和持久性双重标准上,培育抗蚜新品种应考虑蚜虫效应因子与植物的基因间互作以及植物NBS-LRR抗病基因家族可能参与抗蚜免疫反应等观点,以期为揭示植物-蚜虫之间相互作用的分子机制,探索在生产实践中绿色、生态的蚜虫防治新方法提供理论依据。  相似文献   

8.
The cotton aphid (Aphis gossypii) is one of the major pests of okra. Eleven okra varieties collected from farmers' fields in Cameroon and four aphid-resistant accessions from The World Vegetable Center (AVRDC, Asian Vegetable Research and Development Center) were evaluated from October 2012 to March 2013, and another seven aphid-resistant accessions from AVRDC were evaluated from March to July 2013 at AVRDC's Nkolbisson station, Yaoundé, Cameroon. Accessions selected from these trials were screened in an advanced confirmatory screening in autumn (October–December) 2013 in Shanhua, Taiwan. Results showed that none of the farmers' landraces were resistant to aphids. Two accessions (VI041210 and VI033824) with the lowest aphid populations had the highest trichome density. Analysis of various phytochemicals in selected okra accessions revealed that higher nitrogen and potassium levels made the okra accessions susceptible to aphids during the reproductive stage of the plants. Similarly, the total phenol content was lower in moderately resistant accessions during their reproductive stage. Aphid feeding induced an increase in phenols, and to a lesser extent, tannins. Accessions VI041210 and VI033824 could be incorporated into an integrated pest management strategy to enhance productivity of okra.  相似文献   

9.
Since potato leafroll virus multiplies in the green peach aphid,Myzus persicae, the effect of the virus on the biology of its vector was investigated. Observations were made regarding the longevity and the reproduction rate of viruliferous and non-viruliferous aphids on leafroll-diseased and healthy plants ofPhysalis floridana. The same matters were investigated for both viruliferous and non-viruliferous aphids on seedlings of Chinese cabbage (Brassica pekinensis). It was shown that on leafroll-diseased plants ofP. floridana the aphids produced more progeny than on healthy ones, although the average number of progeny produced per day in both cases was almost the same. On healthy Chinese cabbage seedlings there was no difference in average length of the larval and adult stages, number of progeny per aphid, and number of progeny per day, between viruliferous and non-viruliferous aphids. Evidence was obtained that the virus does not influence the development of its vector. Measurements of oxygen consumption of both viruliferous and non-viruliferous aphids point in the same direction.  相似文献   

10.
The severe yellowing disease (amarelão) on melon plants is a serious problem in Brazil, although the causative agent remained unknown for a long time. Recently, recombinant isolates of cucurbit aphid-borne yellows virus (CABYV) were reported as the possible causative agents of this disease on melon plants. Although aphids are known to be the vectors of the common type of CABYV isolates, almost no aphid colony was observed in the major melon fields in Brazil with high incidence of the severe yellowing disease. In contrast, whiteflies are often abundant. Based on this observation, the hypothesis of the transmission of recombinant CABYV by whiteflies was evaluated. After thorough transmission experiments, we found that this recombinant CABYV isolate was transmitted by the whitefly Bemisia tabaci MEAM1, but not by Aphis gossipii. Furthermore, the host response by whitefly-based inoculation in cucurbits and other indicator plants showed differences in host range when compared to the common type of CABYV. Due to its transmissibility by the whitefly and the distant relationship of the P3/P5 protein to CABYV, the name “cucurbit whitefly-borne yellows virus” is proposed for this recombinant CABYV. This is the second report of polerovirus transmission by the whitefly B. tabaci, following the report of pepper whitefly-borne vein yellows virus.  相似文献   

11.
Stability of the aphid transmission phenotype in cucumber mosaic virus   总被引:2,自引:0,他引:2  
Ng  & Perry 《Plant pathology》1999,48(3):388-394
The stability of the aphid transmission phenotype in seven field isolates of cucumber mosaic virus (CMV) was studied, using aphids Aphis gossypii and Myzus persicae . Field isolates, obtained from four vegetable crops, were propagated in squash and Nicotiana glutinosa , and passaged by either aphid transmission or mechanical transfer. All seven isolates were transmissible by both aphids and this aphid transmission phenotype was stable after 20–24 mechanical passages. Upon further mechanical passaging, one of the seven isolates, CMV-2 A1-MT 60x, lost its transmissibility by Myzus persicae but was still transmissible by Aphis gossypii , although at a reduced rate. Isolates maintained by both aphid transmission and mechanical transfer were transmitted more efficiently by Aphis gossypii than by Myzus persicae . A comparison of the RNA profiles showed no major differences among the CMV isolates before and after mechanical passage.  相似文献   

12.
The influence of co‐infection on concentration and accumulation of genetically different isolates of Potato virus Y (PVY) in potato and tobacco plants and the efficiency of transmission by Myzus persicae of PVY isolates from doubly versus singly infected plants were evaluated. The vector ability to simultaneously transmit two virus isolates was examined. Eight PVY isolates represented three strain groups: PVYO (pathotype and serotype O), PVYNW (pathotype N and serotype O), and PVYNTN (pathotype and serotype N). Different diagnostic methods, including DAS‐ELISA, multiplex RT‐PCR, aphid transmission tests and bioassays, were applied to detect the presence of PVY isolates in source and assay plants. Significant reductions in concentrations of certain PVY isolates during co‐infection with other isolates were found both in potato and tobacco plants. The observed effects were both isolate‐ and host‐dependent in form. The highest rates of virus transmission by single aphids were recorded with PVYNTN isolates, and the lowest ones with PVYO isolates. Individual aphids of M. persicae were able to simultaneously transmit two PVY isolates. The frequency of transmission was generally low, but it reached as high as 20% for one of the isolate combinations. The findings presented in the work provide proof for antagonistic within‐plant interactions between isolates of PVY, with some implications of these interactions for virus transmission by aphid vectors. Consequently, this research contributes to a better understanding of the epidemiology of the disease caused by PVY.  相似文献   

13.
The effect of low concentrations of azadirachtin applied systemically through root tissues of rape plants (Brassica napus subsp.napus) on the mortality, period of development, longevity and fecundity of the cabbage aphid (Brevicoryne brassicae L.) (Sternorrhyncha: Aphididae) was studied. The concentrations of azadirachtin A tested were: 0.25, 0.05, 0.005, 0.0005 and 0.00005 mg ml−1. The mortality of cabbage aphid nymphs, particularly during the period of ecdysis, increased significantly with increasing concentrations. Azadirachtin had no effect on the length of the development period of the respective immature stages of cabbage aphids. The average longevity of the cabbage aphids decreased with increasing azadirachtin concentration and time spent feeding. The fecundity of aphids decreased after the application of azadirachtin. http://www.phytoparasitica.org posting May 6, 2004.  相似文献   

14.
This paper reviews the contribution of vector activity and plant age to virus spread in potato crops. Determining which aphid species are vectors is particularly important for timing haulm destruction to minimize tuber infection by potato virus Y (PVY). Alate aphids of more than 30 species transmit PVY, and aphids such asRhopalosiphum padi, that migrate in large numbers before flights of the more efficient vector,Myzus persicae, appear to be important vectors. Differences in methodology, aphid biotypes and virus strains prevent direct comparisons between estimates of vector efficiencies obtained for aphids in different countries in north western Europe. M. persicae is also the most efficient vector of potato leafroll virus (PLRV), but some clones ofMacrosiphum euphorbiae transmit PLRV efficiently toNicotiana clevelandii and potato test plants. The removal of infected plants early in the season prevents the spread of PLRV in cool regions with limited vector activity. The proportion of aphids acquiring PLRV from infected potato plants decreases with plant age, and healthy potato plants are more resistant to infection later in the season. Severe symptoms of secondary leafroll developed on progeny plants of cv. Maris Piper derived from mother plants inoculated with PLRV in June or July of the previous year. Progeny plants derived from mother plants inoculated in August showed only mild symptoms, but the concentration of PLRV in these plants was as high as that in the plants with severe symptoms.  相似文献   

15.
16.
The overall aim of this study was to identify potential associations between aphids and plants as reservoirs of entomophthoralean fungi. A survey of weeds associated with wheat field borders in two different localities was performed over two years, from April 2010 to April 2012 in the Pampeana central region, Argentina. On each sampling date, five individuals of each plant species were randomly selected, and healthy and infected aphids were collected and quantified once a month. The taxonomic identities of both aphid hosts and entomophthoralean fungi were established. Aphid–fungus associations identified as favorable for inclusion in conservation biological control strategies in borders of wheat crops are the following: Sonchus oleraceus (L.)/Hyperomyzus carduellinus (Theobald), Uroleucon sonchi (L.)/Pandora neoaphidis (Remaudière & Hennebert) Humber; S. oleraceus (L.)/H. carduellinus (Theobald)/Zoophthora radicans (Brefeld) Batko; Lamium amplexicaule (L.)/Cryptomyzus korschelti Bôrner/P. neoaphidis, Z. radicans, Entomophthora planchoniana Cornu; Foeniculum vulgare (Miller)/Dysaphis apiifolia (Theobald)/Z. radicans; Morrenia brachystephana Griseb/Aphis nerii Boyer de Fonscolombe/P. neoaphidis and Brassica rapa L./Brevicoryne brassicae (L.)/P. neoaphidis.  相似文献   

17.
Different field isolates of the ‘beet poleroviruses’ Beet mild yellowing virus (BMYV) and Beet chlorosis virus (BChV) (genus Polerovirus, family Luteoviridae) collected in France and Poland were evaluated for transmissibility from and to sugar beet plants by different aphid species. In general, both BMYV and BChV were efficiently transmitted by Myzus persicae and by a French clone of Macrosiphum euphorbiae. In contrast, transmissibility of the two poleroviruses by an English clone of M. euphorbiae was evidently weaker, although the aphid samples contained the virus as demonstrated by RT-PCR. None of the BMYV or BChV isolates was transmitted by Aphis fabae or Myzus ascalonicus. In attempting to correlate biological properties with molecular variations, the RT proteins were sequenced. Some amino acid point variations, presumably affecting aphid transmissibility, were identified.  相似文献   

18.
甘藍蚜、桃蚜和棉蚜的有翅型与无翅型,均能传播大白菜病毒病,其中以甘藍蚜为最主要的传毒介体。一头带毒蚜可使80%的健苗发病;一头无毒蚜經吸毒一分钟的传病率为35%;健苗經一头有毒蚜传毒一分钟,就有10%发病。病毒系非持久性的,蚜虫一次吸毒后的传毒期限为25分钟左右。在烏魯木齐地区,有翅甘藍蚜全年中的迁飞高峯期,都是在7月下旬。証实了这时期与大白菜病毒病的流行,有极密切的关系。当年春、夏季播种的甘蓝和苤藍,尤其是晚甘蓝和晚苤蓝,是大白菜(包括冬蘿卜)苗期的初次毒源和蚜源。初步認为,能感染病毒的几种十字花科及藜科杂草,不是本病病毒和甘藍蚜的重要越夏寄主。  相似文献   

19.
This paper reviews the contribution of vector activity and plant age to virus spread in potato crops. Determining which aphid species are vectors is particularly important for timing haulm destruction to minimize tuber infection by potato virus Y (PVY). Alate aphids of more than 30 species transmit PVY, and aphids such asRhopalosiphum padi, that migrate in large numbers before flights of the more efficient vector,Myzus persicae, appear to be important vectors. Differences in methodology, aphid biotypes and virus strains prevent direct comparisons between estimates of vector efficiencies obtained for aphids in different countries in north western Europe.M. persicae is also the most efficient vector of potato leafroll virus (PLRV), but some clones ofMacrosiphum euphorbiae transmit PLRV efficiently toNicotiana clevelandii and potato test plants. The removal of infected plants early in the season prevents the spread of PLRV in cool regions with limited vector activity. The proportion of aphids acquiring PLRV from infected potato plants decreases with plant age, and healthy potato plants are more resistant to infection later in the season. Severe symptoms of secondary leafroll developed on progeny plants of cv. Maris Piper derived from mother plants inoculated with PLRV in June or July of the previous year. Progeny plants derived from mother plants inoculated in August showed only mild symptoms, but the concentration of PLRV in these plants was as high as that in the plants with severe symptoms.  相似文献   

20.
The effectiveness of two synthetic pyrethroids, cypermethrin and fenvalerate, and of methomyl applied to cabbage plants was tested against the cabbage aphid, Brevicoryne brassicae (L.), and its parasitoid, Diaeretiella rapae (M'Intosh). Dosage rates chosen were equivalent to the manufacturer's maximum and minimum recommendations. Methomyl was considerably more toxic to B. brassicae adults than the two synthetic pyrethroids used. Fenvalerate, at the lowest rate used (71 g ha?1), showed satisfactory effectiveness in reducing aphid adult population on treated plants by c. 77% below that of the control. All insecticides and rates used significantly reduced the emergence of parasitoid adult D. rapae from mummified aphids compared to the control. Fenvalerate clearly had less impact on emergence than cypermethrin or methomyl at any dosage used, while methomyl has appeared to be the most toxic compound in this respect. Longevity of parasitoid adults was highly affected by insecticide application, particularly those adults emerging from mummified aphids exposed to methomyl or cypermethrin. However, only a slight difference in longevity was seen at the lower rate of 71 g ha?1 of fenvalerate. At the lowest dosage rates used, methomyl and cypermethrin reduced the F1-progeny of adult parasitoid females by 70.5% and 58.3%, respectively, of the control, whereas fenvalerate reduced the progeny by only 36.4%.  相似文献   

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