陇东绒山羊周岁母羊主要经济性状遗传力估测

试验测定周岁陇东绒山羊的主要经济性状,采用父系半同胞相关法估测了遗传力。结果表明,陇东绒山羊周岁母羊产绒量、体重、体高、体长、胸围、管围、羊绒直径、绒层高度、羊绒伸直长度的遗传力分别为0.4129、0.3871、0.3732、0.3128、0.3468、0.2873、0.4013、0.3916、0.3908,为陇东绒山羊的科研育种提供了一定的科学依据。     

陇东绒山羊周岁母羊主要经济性状遗传力估测

试验测定周岁陇东绒山羊的主要经济性状,采用父系半同胞相关法估测了遗传力.结果表明,陇东绒山羊周岁母羊产绒量、体重、体高、体长、胸围、管围、羊绒直径、绒层高度、羊绒伸直长度的遗传力分别为0.4129、0.3871、0.3732、0.3128、0.3468、0.2873、0.4013、0.3916、0.3908,为陇东绒山羊的科研育种提供了一定的科学依据.     

陇东绒山羊专门化新品系培育的研究

对陇东绒山羊的选育和提高,组建了陇东绒山羊绒长绒多型和绒细绒密型专门化新品系,其产绒量两品系公母羊分别为586．32±121．39g、467．48±95．16g和579．28±113．26g、456．13±80．78g,和陇东绒山羊公母羊相比,产绒量有一定提高,但差异不显著（P〉0．05）;羊绒细度两品系公母羊分别为14．95±0．83μm、14．89±0．86μm和14．68±0.79μm、14．45±0．81μm,和陇东绒山羊相比,差异不显著（P〉0．05）。     

陇东优质高产绒山羊选育的研究

通过对陇东绒山羊的选育和提高,组建了陇东优质高产绒山羊群,其成年公母羊产绒量分别为693.75±135.29 g和493.46±93.78 g,和陇东绒山羊新品系公母羊相比,产绒量显著提高（P〈0.05）;公母羊羊绒细度分别为15.03±0.85μm和14.56±0.76μm,和陇东绒山羊相比,差异不显著（P〉0.05）;公母羊羊绒的自然长度分别为5.89±0.63 cm和5.26±0.37cm,和陇东绒山羊相比,差异显著（P〈0.05）。因此,陇东优质高产绒山羊产绒量高,羊绒纤维细、长、延伸性好,属优质山羊绒,符合国际羊绒精纺要求并达到了同类纤维理想水平。     

陇东绒山羊发展方略探讨

陇东白绒山羊是上世纪七十年代初引入辽宁绒山羊作为父本杂交改良当地土种黑山羊,历经级进杂交、横交固定、自群繁育三个阶段育成陇东白绒山羊品种类群,在此基础上加大选种选配,突出产绒量及羊绒细度等主要经济性状的选择,推广种羊,扩大数量,于“十五”末育成了以产绒为主、绒肉兼用的陇东绒山羊新品种。截止2006年底,庆阳市境内陇东白绒山羊存栏量已经达到3．7万只,成为当地畜牧业的支柱产业之一。     

陇东绒山羊血液酶(SGPT、SGOT)与产绒量关系的试验

试验测定绒山羊血液酶活性和产绒量并做相关性分析。结果表明 ,陇东绒山羊SGPT活性测定值与产绒量之间存在显著负相关 ,r =- 0 35 9(P <0 0 5 ) ,SGPT活性对产绒量存在显著的直线回归关系 (P <0 0 5 )。SGPT活性可用于间接评估陇东绒山羊羊绒生产水平 ,而SGOT与产绒量之间无显著变化 (P >0 0 5 ) ,影响程度不大     

陇东绒山羊新品种培育的研究

引入辽宁绒山羊杂交改良陇东黑山羊，经过省市县科技人员30余年的联合攻关和科学研究，培育出陇东绒山羊新品种，数量达22162只，其产绒量公、母羊分别为562．50±103．21g和440．4±83．24g，比同龄陇东黑山羊分别增绒397．5g和304．4g，差异极显著（P〈0．01）；比同龄辽陇杂种公母羊分别增绒155．15g和176．08g，差异显著（P〈0．05）；羊绒细度公母羊分别为14．82um和14．89um；羊绒伸直长度分别为87．36mm和81．09mm；强度3．89g；伸度41．14％；含绒率47．46％。符合国际羊绒精纺要求和同类纤维理想水平。     

陇东白绒山羊选育效果分析

引入辽宁绒山羊,杂交改良陇东黑山羊,经过省市县科技人员联合攻关,培育出了适应华池当地自然条件的陇东白绒山羊品种类群羊7 659只,其年产绒量公、母羊分别为528．36 g和361．47 g,比同龄陇东黑山羊分别提高90．48％和160．16％,差异极显著(P<0．01);比同龄辽陇杂种公母羊分别提高29．71％和36．75％,差异显著(P<0．05);羊绒细度公母羊分别为14．89μm和14．75μm;羊绒长度公母羊分别为8．22 cm和7．25 cm,符合国际羊绒精纺要求。     

陇东绒山羊专门化品系选育研究报告

选育和提高陇东绒山羊的生产性能,以陇东绒山羊为试验动物,采用数量遗传学和现代繁殖新技术,在甘肃东部地区选育出陇东绒山羊专门化新品系,数量达到18 685只,其中绒长型9 632只,绒细型9 053只。新品系被毛全白,以产绒为主,绒肉兼用,两品系特一级成年公母羊产绒量分别为626.49 g、506.87 g和601.67 g、465.76 g;羊绒细度分别为15.02μm、14.82μm和14.49μm、14.25μm;羊绒伸直长度分别为90.37 mm、75.82 mm和85.41 mm、79.78 mm;春季体重两品系公母羊分别为36 kg和28 kg以上,屠宰率为47.68%,经产母羊产羔率105%。选育结果陇东绒山羊专门化品系外貌特征一致,遗传性能稳定,白色个体稳定在97.6%以上,繁殖性能良好,其中双羔率占约5%。陇东绒山羊专门化新品系具有体质结实,角型整齐,被毛全白,产绒量高,羊绒品质优良,耐粗饲,抗病力强,适应干旱环境条件等优点。     

陇东绒山羊专门化品系培育效果与分析

为了进一步培育优质高产陇东绒山羊群体,利用引进的绒细型和绒长型种公羊进行分区域杂交改良和品系繁育,培育出适宜陇东干旱半干旱自然生态条件的陇东绒山羊专门化品系。培育结果表明:绒细型特一级成年公母羊平均产绒量分别为601.67g和465.76g,体重分别为36.16kg和29.31kg,羊绒细度分别为14.49μm和14.25μm;绒长型特一级成年公母羊平均产绒量分别为626.49g和506.37g,体重分别为37.74kg和29.65kg,羊绒细度分别为15.02μm和14.82μm。同时利用微卫星DNA技术研究了9对基因座位在陇东绒山羊群体中的遗传多样性,通过标记多态性与生产性状的最小二乘线性模型,进行了分子标记与经济性状的相关分析,并在这些位点上找到经济性状优势基因型以及对相应性状具有正效应或负效应的等位基因。     

癸氧喹酯干混悬剂含量测定的改进

采用高效液相色谱法测定癸氧喹酯干混悬剂的含量,在2-250μg/mL范围内,峰面积的常用对数与进样量浓度的常用对数呈良好的线性关系,R^2=1(n=5),平均回收率为99.24%~99.51%,RSD在0.05%~0.28%。此方法分析时间短,样品前处理简便、定量结果准确,重现性好,结果满意,为其质量控制提供了依据。     

商会自治的基石——商会自治规范研究

在现代法律秩序中,商会自治规范是制定法的基础和必要的补充,甚至在某些方面替代了制定法;商会自治规范主要包括商会组织规范、行为规范、惩罚规范以及争端解决规范等;其效力仅及于其内部成员;商会自治规范和制定法之间存在冲突,但也存在整合的基础。     

猪毛色遗传的研究进展

本文概述了猪的毛色类型、猪的毛色遗传模式,着重综述了猪毛色基因分子基础的研究进展,指出存在问题并就未来发展方向做了思考。     

中华人民共和国农业部公告 第267号

为贯彻落实《兽药生产质量管理规范》(简称《兽药GMP》),进一步推动兽药GMP实施进程,我部制定了《兽药生产质量管理规范检查验收办法》,现予公告。本公告自2003年6月1日起施行。附件:兽药生产质量管理规范检查验收办法二○○三年四月十日第一章 总则 第一条 为推动《兽药生产质量管理规范》(以下简称兽药GMP)的实施,规范兽药GMP检查验收工作,制定本办法。 第二条 农业部负责全国兽药GMP管理和检查验收工作;负责制修订兽药GMP检查验收管理规定;负责兽药GMP检查员队伍建设和监督管理工作,负责国际兽药贸易中GMP互认工作。 …     

鸡败血支原体垂直传播模式及其阻断方法

以国际标准强毒Ｒ株人工感染非免疫产蛋鸡,定时扑杀,分别从鼻窦、眶下孔、气管、肺、气囊、卵巢和输卵管分离ＭＧ,并收集感染鸡所产蛋分离ＭＧ。结果表明,人工感染４８小时后上、下呼吸道及肺已被全面感染,９６小时气囊已被感染,１２０小时输卵管已能分离到ＭＧ,卵巢始终分离不到ＭＧ。人工感染鸡自１４４小时便能在其所产蛋中分离出ＭＧ。药物治疗能在７２小时内消除感染,油乳剂苗则需２４天后逐渐降低蛋内ＭＧ分离率,药物卵内注射、种蛋药浴、高温处理均能杀死卵内ＭＧ,但以研制的种蛋浸泡剂药浴效果为最好。     

Comparison of 2 methods of centesis of the bursa of the biceps brachii tendon of horses

REASONS FOR PERFORMING STUDY: Centesis of the bicipital bursa using an 8.9 cm long spinal needle has been reported but the alternative of employing a 3.8 cm long hypodermic needle requires validation. OBJECTIVE: To compare the efficacy of 2 different methods of centesis of the bicipital bursa and to evaluate the usefulness of ultrasonographic imaging to determine the location of solution administered when centesis of the bursa is attempted. METHODS: For Trial 1, 6 clinicians, who had no previous experience of centesis of the bicipital bursa, attempted to inject a solution composed of an aqueous radiopaque contrast medium and physiological saline solution (PSS) into the bicipital bursae of 2/12 horses using the previously described distal approach to inject one bursa and a proximal approach to inject the contralateral bursa. The bicipital tendon and bursa were examined ultrasonographically before and after injection; and both shoulders were examined radiographically to identify the location of the medium. In Trial 2, another 6 clinicians, also with no previous experience of centesis, repeated Trial 1, using 6 horses, but the radiopaque contrast medium was mixed with air instead of PSS. RESULTS: Accuracy of centesis using the proximal approach was 39% and that of the distal approach 28%. Ultrasonographic examination of the shoulder allowed the location of solution and air to be accurately predicted in all 12 shoulders examined. CONCLUSIONS: Clinicians who have had no previous experience performing centesis of the bicipital bursa are unlikely to be successful in centesis using either approach. Radiographic examination after injecting a radiopaque contrast medium may be necessary to assess the success of centesis especially if bursal fluid is not obtained during centesis. Injecting air along with the radiopaque contrast medium provides more accurate ultrasonographic confirmation of centesis and better radiographic definition than does injection without air.     

不同样本商品蜂蜜中硒含量的测定

用硝酸和高氯酸消化蜂蜜,使硒游离出来,在微酸性环境下,硒和2,3-二氨基萘(DAN)生成有较强荧光的物质,用环己烷萃取,在激发波长378nm,荧光波长518nm处测定其荧光强度。蜂蜜中硒含量范围:0.10～0.82μg/g。表明:蜂蜜应视为天然富硒营养品。     

乳酸杆菌益生作用机制的研究进展

乳酸杆菌作为益生菌广泛用于人和动物。本文综述了乳酸杆菌改善宿主健康的机制。乳酸杆菌可通过产生抗菌物质如乳酸、过氧化氢、细菌素,或者通过竞争营养或肠道黏附位点来抑制致病菌;通过诱导黏附素的分泌或阻止细胞凋亡而增强肠道的屏障功能,从而保护肠道。文章重点讨论了乳酸杆菌表面成分（表面蛋白、脂磷壁酸和肽聚糖）与肠道受体（Ｃ型凝集素受体、Ｔｏｌｌ样受体和 Ｎｏｄ样受体）,阐述了他们结合后启动免疫调节信号,调控肠道免疫功能以发挥改善健康作用的机制。     

Effects of size of ingestively masticated fragments of plant tissues on kinetics of digestion of NDF

Ingestively masticated fragments were collected and sized via sieving. Different sizes of esophageal masticate and ruminal digesta fragments, and ground fragments of larger masticated pieces were incubated in vitro, and undigested NDF remaining at intervals of up to 168 h of incubation was determined. The ruminal age-dependent time delay (tau) for onset of digestion of NDF was positively correlated (P < 0.004) with the mean sieve aperture estimated to retain 50% of the fragments between successive sieve apertures (MRA). Degradation rate of potentially degradable NDF (PDF) and level of indigestible NDF were not related (P > 0.10) to MRA of masticated and ground fragments. Estimates of tau were positively related to MRA, with slopes of bermudagrass < corn silage < ruminal fragments of corn silage. It was concluded that fragment size-, and consequently, ruminal age-dependent onset of PDF degradation of a mixture of different fragment sizes results in an age-dependent rate of degradation of the more rapidly degrading of two subentities of PDF. Models are proposed that assume a tau before onset of simultaneous degradation of PDF from two pools characterized as having gamma-modeled age-dependency and age-constant rates. The ruminal age-dependent pool seems to be associated with the faster-degrading pool, and its rate parameter increases with range in MRA in the population of fragments. Conceptually, the ruminal age-dependent rate parameter for PDF degradation seems to represent a composite of several effects: 1) effects of the size-dependent tau; 2) range in MRA of the population of ingestively masticated fragments; and 3) subentities of PDF that degrade via more rapid age-dependent rates compared with subentities of PDF that degrade via age-constant rates. The estimated fractional rates of ruminative comminution of ingestively masticated fragments (0.060 to 0.075/h) were of a magnitude similar to the mean fractional rates of PDF digestion (0.030 to 0.085/h), which implies that ruminative comminution may be first-limiting to fractional rate of PDF digestion. The in vivo roles of ingestive and ruminative mastication of fragments on PDF degradation must be considered in any kinetic system for estimating PDF digestion in the rumen. These results and others in the literature suggest that the rate of surface area exposure rather than intrinsic chemical attributes of PDF may be first-limiting to degradation rate of PDF in vivo.