Genetic Diversity in Wild Populations of Poacynum hendersonii from Inland Arid Regions of Northwest China

[Objective] Study on the genetic diversity in wild populations of Poacynum hendersonii.[Method] Random amplified polymorphic DNA(RAPD)technique was employed to analyze the genetic diversity in five wild populations of P.hendersonii sampled from Xinjiang,Gansu and Qinghai provinces.[Result] Totally 165 clear and repeatable bands were generated in RAPD reaction by using 20 primers screened from 80 primers,of which 110 were polymorphic,accounting for 66.67%.At species level,Nei's gene diversity index(H),Shannon's information index(I)and genetic differentiation coefficient(Gst)were 0.220 5,0.304 7 and 0.908 2,respectively.P.hendersonii germplasm resources share a high level of genetic diversity,and genetic differentiation mainly exists among the populations.Results from genetic distances and cluster analysis showed that relationships among P.hendersonii populations were to some extent related with their geographical and climatic characters.[Conclusion] This study suggests that the conservation of P.hendersonii should focus on the protection of many populations,particularly the Qinghai population.     

中国西北内陆干旱区大叶白麻野生居群遗传多样性研究(英文)

[Objective] Study on the genetic diversity in wild populations of Poacynum hendersonii.[Method] Random amplified polymorphic DNA(RAPD)technique was employed to analyze the genetic diversity in five wild populations of P.hendersonii sampled from Xinjiang,Gansu and Qinghai provinces.[Result] Totally 165 clear and repeatable bands were generated in RAPD reaction by using 20 primers screened from 80 primers,of which 110 were polymorphic,accounting for 66.67%.At species level,Nei’s gene diversity index(H),Shannon’s information index(I)and genetic differentiation coefficient(Gst)were 0.220 5,0.304 7 and 0.908 2,respectively.P.hendersonii germplasm resources share a high level of genetic diversity,and genetic differentiation mainly exists among the populations.Results from genetic distances and cluster analysis showed that relationships among P.hendersonii populations were to some extent related with their geographical and climatic characters.[Conclusion] This study suggests that the conservation of P.hendersonii should focus on the protection of many populations,particularly the Qinghai population.     

中国西北内陆干旱区大叶白麻野生居群遗传多样性研究(英文)

[Objective] Study on the genetic diversity in wild populations of Poacynum hendersonii.[Method] Random amplified polymorphic DNA(RAPD)technique was employed to analyze the genetic diversity in five wild populations of P.hendersonii sampled from Xinjiang,Gansu and Qinghai provinces.[Result] Totally 165 clear and repeatable bands were generated in RAPD reaction by using 20 primers screened from 80 primers,of which 110 were polymorphic,accounting for 66.67%.At species level,Nei's gene diversity index(H),Shannon's information index(I)and genetic differentiation coefficient(Gst)were 0.220 5,0.304 7 and 0.908 2,respectively.P.hendersonii germplasm resources share a high level of genetic diversity,and genetic differentiation mainly exists among the populations.Results from genetic distances and cluster analysis showed that relationships among P.hendersonii populations were to some extent related with their geographical and climatic characters.[Conclusion] This study suggests that the conservation of P.hendersonii should focus on the protection of many populations,particularly the Qinghai population.     

新疆红花主要栽培品种遗传多样性的RAPD分析(英文)

[Objective] Study on the genetic diversity in main cultivars of safflower distributing in Xinjiang Uighur Autonomous Region by means of RAPD makers.[Method] Genomic DNAs of 29 safflower accessions from Xinjiang Uighur Autonomous Region were extracted for PCR amplification using 20 RAPD primers.[Result] Totally 156 bands were amplified,among which 144 bands were polymorphic(accounting for 92.31%),indicating that safflower is endowed with plentiful genetic diversity.Based on the DNA fingerprint,the 29 safflower accessions were grouped into four populations,the classification results may be not related with ecological regionality.[Conclusion] RAPD technique is an available tool to analyze the genetic diversity of safflower germplasm at molecular level.     

新疆红花主要栽培品种遗传多样性的RAPD分析(英文)

[Objective] Study on the genetic diversity in main cultivars of safflower distributing in Xinjiang Uighur Autonomous Region by means of RAPD makers.[Method] Genomic DNAs of 29 safflower accessions from Xinjiang Uighur Autonomous Region were extracted for PCR amplification using 20 RAPD primers.[Result] Totally 156 bands were amplified,among which 144 bands were polymorphic(accounting for 92.31%),indicating that safflower is endowed with plentiful genetic diversity.Based on the DNA fingerprint,the 29 safflower accessions were grouped into four populations,the classification results may be not related with ecological regionality.[Conclusion] RAPD technique is an available tool to analyze the genetic diversity of safflower germplasm at molecular level.     

Genetic Diversity in Main Cultivars of Safflower in Xinjiang Uighur Autonomous Region Based on RAPD Analysis

[Objective] Study on the genetic diversity in main cultivars of safflower distributing in Xinjiang Uighur Autonomous Region by means of RAPD makers.[Method] Genomic DNAs of 29 safflower accessions from Xinjiang Uighur Autonomous Region were extracted for PCR amplification using 20 RAPD primers.[Result] Totally 156 bands were amplified,among which 144 bands were polymorphic(accounting for 92.31%),indicating that safflower is endowed with plentiful genetic diversity.Based on the DNA fingerprint,the 29 safflower accessions were grouped into four populations,the classification results may be not related with ecological regionality.[Conclusion] RAPD technique is an available tool to analyze the genetic diversity of safflower germplasm at molecular level.     

Analysis on Genetic Relationship of Oxya chinensis and Oxya japonica from Xuzhou and Pingshan, China

Genetic relationship among Oxya chinensis populations and Oxya japonica populations collected from Xuzhou City, Jiangsu Province and Pingshan County, Hebei Province, China were analyzed by random amplified polymorphic DNA （RAPD） markers. A total of 125 DNA bands ranging from 200 to 2 200 bp were amplified by 10 random primers in DNA samples from 43 grasshopper individuals. One hundred and twenty-three （99%） of these bands were polymorphic. Shannon＇s index showed that the genetic, diversity within O. chinensis （0.3432） was higher than that of O. japonica （0.2781）. Nei＇s genetic distance between O. chinensis population and O. japonica population from the same area was less than that between populations from two different areas. The dendrogram based on Nei＇s genetic distance of RAPD markers was constructed using the unweighted pair group method with the arithmetic average （UPGMA） and Neighbor-Joining （NJ） methods. Cluster analysis indicated that all the individuals were grouped into two main clusters. O. chinensis populations from Xuzhou and Pingshan formed one cluster, and O. japonica populations from the two regions belonged to another cluster. The results demonstrated that RAPD can detect within species, and among closely related species. polymorphisms to distinguish minor difference among individuals     

Genetic Diversity Analysis of Cultured Populations of Jade Perch （Scortum bacoo） in China Using AFLP Markers

Genetic diversity of three cultured populations of jade perch （Scortum bacoo） are studied using amplified fragment length polymorphism （AFLP） markers, which is Guangzhou（GZ） population, Foshan （FS） population and Qingdao（QD） population. Nine primer combinations were used and 385 fragments were detected. Among the 385 fragments, 80 bands （20.78%） were polymorphic. And it can be speculated that the genetic diversity of the three cultured populations of jade perch was very poor according to the gene genetic diversity among populations （Ht）, the gene genetic diversity within populations （Hs） and Shannon-Weiner index （I）. From gene differentiation （Gst）, genetic distance （D）, genetic similarity （5）, and UPGMA analysis, it is found that the relationship among the three populations is very closed, and the difference in genetic diversity among the three populations is not significant. Further clustering relationships of the jade perch cultured populations also are correlated to historical-breeding observations and genetic relationships. The GZ population clustered together with the QD population first, then with FS population.     

Genetic Variation in Triticum turgidum L.ssp.turgidum Landraces from China Assessed by EST-SSR Markers

It was helpful for the wheat improvement to evaluate the genetic resources of Triticum turgidum L. ssp. turgidum landraces. In this study, 68 turgidum landraces accessions, belonging to four geographic populations in China, were investigated by using EST-SSR markers. A total of 63 alleles were detected on 22 EST-SSR loci, and the number of alleles on each locus ranged from 1 to 5, with an average of 2.9. The results of the analysis of molecular variance （AMOVA） indicated that 92.5% of the total variations was attributed to the genetic variations within population, whereas only 7.5% variations among populations. Although the four populations had similar genetic diversity parameters, Sichuan population was yet distinguished from other populations when comparing the population samples in pairs. Significant correlations were detected by the statistic analysis among six genetic diversity parameters among each other. The selection difference between heterozygosty and homozygosty was also observed among different EST-SSR locus. The genetic similarity （GS） ranged from 0.18 to 0.98, with the mean of 0.72, and all accessions could be clustered into 7 groups. The dendrogram suggested that the genetic relationships among turgidum accessions evaluated by EST-SSR markers were unrelated to their geographic distributions. These results implied that turgidum landraces from China had the unique characters of genetic diversity.     

Genetic Diversity Analysis Among Populations of Mink from China by Inter-simple Sequence Repeat （ISSR） Markers

Inter-simple Sequence Repeat （ISSR） analysis was applied to assess the genetic diversity within and among five populations of mink from Liaoning Province. A total of 20 primers were screened, five selected primers produced 35 discernible bands, with 30 （85.71%） being polymorphic, indicating high genetic diversity at the species level. The highest genetic diversity was observed in the brown mink population, whereas the lowest diversity was found in the standard-pitchy mink population. Based on genetic distance （1972）, a dendrogram was constructed by using UPGMA algorithm, and five populations were divided into two major groups. Group I consisted of only the standard-pitchy mink population, and Group II included other four populations, in Group II, sapphire mink was close to brown mink population. The results of genetic differentiation indicated that the genetic differentiation degree between populations was lower and the genetic variation primarily came from within populations. This paper showed that ISSR technique was a reliable tool that could be used to study genetic diversity in the mink.     

芨芨草遗传多样性研究

研究芨芨草的遗传多样性,探讨不同地理种群的遗传分化与地理位置及生境的关系,以期为芨芨草种质资源的开发、利用与保存提供参考。     

芨芨草遗传多样性研究

[目的]研究不同产地芨芨草间遗传多样性。[方法]用随机扩增多态DNA(RAPD)标记分别对芨芨草进行7个居群间和2个居群内的遗传多样性分析。[结果]芨芨草在居群间遗传上的聚类与地理位置直接相关,居群内的遗传分化与生境相关。[结论]PAPD技术可用芨芨草遗传多样性研究。     

不同来源辣椒疫霉菌的遗传多样性分析

[目的]探索来自辣椒寄主和土壤的辣椒疫霉的遗传多样性。[方法]通过利用12个10碱基随机引物对来自我国4个不同地理区域的22个辣椒疫霉菌株的亲缘关系进行RAPD分析。[结果]受试22个菌株共产生101条谱带,其中多态性为99条,占98.02%,说明受试辣椒疫霉菌具有丰富的遗传多样性。根据引物扩增的DNA指纹图谱,运用UPGMA分析法,以遗传相似系数0.5为阈值,将供试22个菌株划分为3个遗传聚类组(Ⅰ、Ⅱ、Ⅲ)。RAPD标记技术分析表明,来辣椒寄主和土壤的菌株的全基因组DNA扩增图谱差异很大。[结论]供试菌株具有丰富的遗传多样性,来自不同遗传背景的菌株差异显著,聚类组的划分与菌株的来源有一定的相关性。     

中国西北内陆干旱区大叶白麻野生居群遗传多样性研究

[目的]研究大叶白麻野生居群的遗传多样性。[方法]采用RAPD技术对分布于新疆、甘肃和青海的5个大叶白麻野生居群的遗传多样性水平进行检测。[结果]经筛选的20条引物共扩增出165条清晰、重复性好的DNA条带,其中多态性条带为110条,多态位点百分率(PPB)为66.67%。居群间Nei′s多样性指数为0.2205,Shannon’s信息指数为0.3047,遗传分化系数(Gst)为0.9082。大叶白麻具有较高的遗传多样性水平,遗传分化主要发生在居群间。聚类分析显示居群间的相似度与分布区的地理和气候特征相关。[结论]在利用大叶白麻资源的同时,应根据各居群的品质和遗传特征,对这些居群尤其是青海居群加以保护。     

RAPD法研究孝感荸荠和野生荸荠的遗传差异性

[目的]采用RAPD法研究孝感荸荠和野生荸荠的遗传差异性。[方法]利用随机扩增多态性DNA(RAPD)技术,对地方栽培种孝感荸荠、野生荸荠、蒲草和扁杆藨草的基因组DNA进行了遗传差异性分析。[结果]共筛选出841、842、807和840 4个随机引物,其中841随机引物的扩增产物多态性明显,获得清晰、重复性好的条带。聚类分析结果显示,栽培荸荠与野生荸荠的亲缘性相对于蒲草与扁杆藨草而言较近。[结论]为培植优质荸荠新品种提供了理论依据。     

新疆红花主要栽培品种遗传多样性的RAPD分析

[目的]利用RAPD技术分析新疆红花栽培品种遗传多样性。[方法]提取29份新疆红花栽培品种的基因组DNA并利用所筛选的20条RAPD引物进行PCR扩增。[结果]共扩增出156条带,其中144条带具有多态性,多态性条带占92.31%,说明新疆的红花具有较丰富的遗传多样性;根据引物扩增出的DNA指纹图谱,运用UPGMA分析法可将29份红花种质聚类划分为4个主要类群,该类群划分结果与红花的生态地域性可能不存相关性。[结论]该方法可用于在分子水平上分析红花品种的遗传多样性。