Hydrochloric acid (0.1M) and dtpa extractable and total iron and manganese in basaltic soil profiles of the Nigerian savanna

Abstract

The contents of 0.1 M HCl and DTPA extractable and total iron (Fe) and manganese (Mn) were determined in soil profiles developed on four groups of basaltic parent materials, namely, the Newer‐, Older‐, Lateritized‐Older‐, and Biu‐basalts. HCl‐and DTPA‐extractable and total Fe in the soils ranged from 15.0 to 66.3 mg/kg, 1.1 to 26. 7 mg/kg, and 4.50 to 10.50%, respectively, with corresponding means of 37.5 mg/kg, 9.5 mg/kg, and 7.33%. The corresponding forms of Mn ranged from 1.1 to 89.0 (mean, 39.8) mg/kg, 0.3 to 114.7 (mean, 24.3) mg/kg and 34 to 1010 (mean, 346) mg/kg, respectively. The soils were high in total Fe and Mn and sufficient in the available forms (HCl‐and DTPA‐extractable forms). Total Fe and Mn were neither related to the available forms or to soil properties tested. The available forms were, however, related one with another and largely associated with organic matter.     

Extractable Micronutrients Status of Soils in a Plinthitic Landscape at Zaria,Nigeria

Desilication and leaching are processes that accompany plinthilization, leading to nutrient depletion. Soils from 12 profiles in a plinthitic landscape were analyzed for extractable micronutrients [iron (Fe), zinc (Zn), manganese (Mn), and copper (Cu)]. Soils of the landscape from crestal to lower‐slope position contain plinthite in the profile, whereas those of the valley floor are devoid of plinthite. The micronutrients were extracted using diethylenetriaminepentaacetic acid (DTPA) and 0.1 M hydrochloric acid (HCl). The results showed that 0.1 M HCl extracted more of the micronutrients than DTPA. The DTPA‐extractable Fe, Zn, Mn, and Cu in all the soils ranged from 1.15 to 12.44 (mean, 3.69); 0.71 to 2.75 (mean, 1.86); trace 12.44 (mean, 3.35), and trace 3.76 (mean, 0.63) mg kg^?1, respectively. The DTPA‐extractable micronutrient contents were generally greater than the critical available level (4.5 mg kg^?1 for Fe, 0.8 mg kg^?1 for Zn, 1.0 mg kg^?1 for Mn, and 0.2 mg kg^?1 for Cu). The 0.1 M HCl‐extractable micronutrients in the landscape ranged from 8.00 to 30.40 (mean, 15.19); 0.30 to 6.49 (mean, 1.35); 1.00 to 27.20 (mean, 7.74); and 0.26 to 15.0 (mean, 2.77) mg kg^?1 for Fe, Zn, Mn, and Cu, respectively. Both DTPA‐ and 0.1 M HCl‐extractable micronutrients were generally lower in the plinthitic horizons than in the nonplinthitic horizons and higher in the Ap than the subsoil horizons. Correlation analysis showed a significant relationship between DTPA‐Fe and DTPA‐Mn, Cu, and organic carbon (r = 0.913**, 0.411**, and 0.385**). There was a significant and positive relationship between 0.1 M HCl‐extractable Mn and organic carbon (C), total nitrogen (N), and available phosphorus (P) (r = 0.413**, 0.337**, and 0.350**, respectively).     

Distribution of Total and DTPA‐Extractable Zinc,Copper, Manganese,and Iron in Vertisols of India

Abstract

Vertisols of India are developed over isohyets of 600 to 1500 mm, and their chemical cycles are set by drainage, landforms, and particle size, which results in variable pedogenic development within the otherwise homogeneous soils. The purpose of this study was to identify pedogenic processes in the distribution of total and DTPA‐extractable zinc (Zn), copper (Cu), manganese (Mn), and iron (Fe). The soils are developed over basaltic parent material of Cretaceous age. Soil samples were drawn from genetic horizons of the 13 benchmark profiles and analyzed by using HF–HClO₄ acid for total and DTPA extraction. Correlation coefficients were calculated taking all samples together. The total concentration varied from 24 to 102 mg kg^?1 for Zn, 21 to 148 mg kg^?1 for Cu, 387 to 1396 mg kg^?1 for Mn, and 2.36 to 9.50% for Fe. Their variability was proisotropic and haplodized, and their concentrations increased with advancing isohyets. Within the isohyets, hindrance in drainage caused retention of Zn and Cu but loss of Fe. The piedmont soils had more Fe than alluvium soils. The spatial distribution of total contents of Zn, Cu, and Fe was influenced by the pedogenic processes associated with Haplusterts but not with provenance materials. Surface concentrations of the elements by biotic lifting and/or harvest removal were negated by the pedoturbation that further contributed to the irregular distribution of the elements in the profiles. Total Zn and total Cu had positive coefficients of correlations with coarse clay, whereas total Mn and total Fe were positively correlated with fine clay. The DTPA‐extractable forms were functions of isohyets and drainage and showed association with organic carbon content and coarse clay.     

Accumulation of copper in soils and leaves of tomato plants in greenhouses in Turkey

Copper (Cu)‐containing fertilizers, fungicides, and bactericides has been used extensively in the greenhouses in the Antalya province in recent decades with the use of such materials tending to increase year by year. The level of DTPA‐extractable Cu was determined in 210 soils (0–20 and 20–40 cm depth) and 105 leaf samples obtained from 105 tomato greenhouses. The DTPA‐extractable Cu contents of the soils taken from the 0–20 depth ranged between 0.76 and 88.03 mg kg^‐1 (mean 7.79 mg kg^‐1). The percentage of soils containing DTPA‐extractable Cu greater than the critical toxicity level (20 mg kg^‐1) was 8.1. The Cu content of tomato leaf samples ranged between 2.4 and 1,490 mg kg^‐1 (mean 166.5 mg kg^‐1). The Cu concentration in leaf samples was very high due to the intensive use of foliar applied Cu‐containing chemicals. As a consequence, 24.8% of the leaf tomato samples analyzed contained over 200 mg Cu kg^‐1, the maximum accepted tolerance level. Therefore, it may be necessary to reduce the use of Cu‐containing pesticides and fertilizers, or at least reduce the amount of Cu‐containing fertilizers being used in those greenhouses where Cu‐containing pesticides have been or are being used.     

Extractability and plant uptake of heavy metals in alum shale soils

Abstract

Fifty soil samples (0–20 cm) with corresponding numbers of grain, potatoes, cabbage, and cauliflower crops were collected from soils developed on alum shale materials in Southeastern Norway to investigate the availability of [cadmium (Cd), copper (Cu), zinc (Zn), lead (Pb), nickel (Ni), and manganese (Mn)] in the soil and the uptake of the metals by these crops. Both total (aqua regia soluble) and extractable [ammonium nitrate (NH₄NO₃) and DTPA] concentrations of metals in the soils were studied. The total concentration of all the heavy metals in the soils were higher compared to other soils found in this region. Forty‐four percent of the soil samples had higher Cd concentration than the limit for application of sewage sludge, whereas the corresponding values for Ni, Cu, and Zn were 60%, 38%, and 16%, respectively. About 70% the soil samples had a too high concentration of one or more of the heavy metals in relation to the limit for application of sewage sludge. Cadmium was the most soluble of the heavy metals, implying that it is more bioavailable than the other non‐essential metals, Pb and Ni. The total (aqua regia soluble) concentrations of Cd, Cu, Zn, and Ni and the concentrations of DTPA‐extractable Cd and Ni were significantly higher in the loam soils than in the sandy loam soils. The amount of NH₄NCyextractable metals did not differ between the texture classes. The concentrations of DTPA‐extractable metals were positively and significantly correlated with the total concentrations of the same metals. Ammonium nitrate‐extractable metals, on the other hand, were not related to their total concentrations, but they were negatively and significantly correlated to soil pH. The average concentration of Cd (0.1 mg kg^‐1 d.w.) in the plants was relatively high compared to the concentration previously found in plants grown on the other soils. The concentrations of the other heavy metals Cu, Zn, Mn, Ni, and Pb in the plants were considered to be within the normal range, except for some samples with relatively high concentrations of Ni and Mn (0–11.1 and 3.5 to 167 mg kg‘¹ d.w., respectively). The concentrations of Cd, Cu, Zn, Ni, and Mn in grain were positively correlated to the concentrations of these respective metals in the soil extracted by NH₄NO₃. The plant concentrations were negatively correlated to pH. The DTPA‐extractable levels were not correlated with plant concentration and hence DTPA would not be a good extractant for determining plant availability in these soils.     

Zinc in wheat grain as affected by nitrogen fertilization and available soil zinc

Greenhouse and field experiments were conducted to determine the influence of nitrogen (N) fertilization and DTPA‐extractable soil zinc (Zn) on Zn concentration in wheat (Triticum aestivum L., cv. Pioneer 2375) grain. Application of zinc sulfate (ZnSO₄) in the range of 0 to 8 mg Zn kg^‐1 increased linearly DTPA‐extractable Zn in an incubated calcareous soil from 0.3 to 5.0 mg kg^‐1. Application of these rates of ZnSO₄ to the same soil under greenhouse conditions increased Zn concentration of wheat grain from 26 to 101 mg kg^‐1. The influence of 134 kg urea‐N ha^‐1 on Zn concentration in wheat grain at eight field sites, with DTPA‐extractable soil Zn levels ranging from 0.3 to 4.9 mg kg^‐1, was studied. Nitrogen fertilizer increased wheat‐grain yields in four of the eight experiments but had little effect on grain‐Zn concentration. Grain‐Zn concentration ranged from 31 to 45 mg kg^‐1 in N‐fertilized plots at the various sites and was related (r=0.74*) to DTPA‐extractable soil Zn.     

Distribution of Forms of Copper and their Association with Soil Properties and Uptake in Major Soil Orders in Semi-arid Soils of Punjab,India

Profiles of semi-arid-zone soils in Punjab, northwestern India, were investigated for different forms of copper (Cu), including total Cu, diethylenetriaminepentaacetic acid (DTPA)–extractable Cu, soil solution plus exchangeable Cu, Cu adsorbed onto inorganic sites, Cu bound by organic sites, and Cu adsorbed onto oxide surfaces. When all soils were considered, total Cu content ranged from 7 to 37 mg kg^?1, while DTPA-extractable and soil solution plus exchangeable Cu contents ranged from 0.30 to 3.26 mg kg^?1 and from 0.02 to 0.43 mg kg^?1, respectively. Copper adsorbed onto inorganic sites ranged from 0.62 to 2.6 mg kg^?1 and that onto oxide surfaces ranged from 2.0 to 13.2 mg kg^?1. The Cu bound by organic sites ranged from 1.2 to 12.2 mg kg^?1. The magnitudes of different forms of Cu in soils did not exhibit any consistent pattern of distribution. Organic matter and size fractions (clay and silt) had a strong influence on the distribution of different forms of Cu. The content of all forms of Cu was generally greater in the fine-textured Alfisols and Inceptisols than coarse-textured Entisols. Soil solution plus exchangeable Cu, Cu held onto organic sites, and and Cu adsorbed onto inorganic sites (crystalline) had significant positive correlations with organic carbon and silt contents.The DTPA Cu was positively correlated with organic carbon, silt, and clay contents. Total Cu content strongly correlated with silt and clay contents of soils. Among the forms, Cu held on the organic site, water soluble + exchangeable Cu, and Cu adsorbed onto oxide surface were positively correlated with DTPA-extractable Cu. The DTPA-extractable Cu and soil solution plus exchangeable Cu seems to be good indices of Cu availability in soils and can be used for correction of Cu deficiency in the soils of the region. The uptake of Cu was greater in fine-textured Inceptisols and Alfisols than coarse-textured Entisols. Among the different forms only DTPA-extractable Cu was positively correlated with total uptake of Cu.     

Micronutrient cation survey of lowland rice in sierra leone

Abstract

Lowland rice plants were sampled at two growth stages and analyzed for Zn, Cu, Mn, and Fe. Most of the sites were deficient in Cu and one‐third of the sites were rated deficient in Zn. All sites were in the adequate range with respect to Mn. Many Fe values were in the excess to toxic range. Average content of micronutrient cations in rice plants was uniformly greater 30 days after the rice was transplanted than 60 days after transplanting.

Correlations between extractable Mn in lowland soils and Mn in associated rice plants were highly significant with the DTPA extractant and significant with the HCl method, both 30 and 60 days after the rice was transplanted. With HCl, extractable Fe in lowland soils was highly correlated with Fe in associated rice plants, but this relationship was not as close with the DTPA extractant.

In lowland soils, extractable Zn increased significantly and consistently with increases in Cu and Mn extracted with DTPA and HCl. Extractable Zn also increased significantly with increases in Fe when the HCl extractant was used, but not with DTPA.     

Labile pools of copper and zinc in calcareous soils as measured by DTPA and plant extraction

Abstract

Labile pools of Cu and Zn were measured on two calcareous soils from Saudi Arabia, using successive extraction with DTPA, and successive cropping followed by extraction with DTPA after cropping. The examined soils differed in their ability to supply Cu and Zn. The first DTPA extraction removed a major proportion of the labile pool, particularly in the sandy soil. The two soils showed a general decrease in extractable Cu and Zn with progressive extractions, with DTPA extractable Cu declining more rapidly than Zn. DTPA extractable Cu and Zn determined after cropping were highly correlated with DTPA extractable Cu and Zn values obtained after successive extractions. The results gave evidence on both the contribution of element dissolution from insoluble forms with progressive extraction or cropping, and the usefulness of the DTPA extractant for monitoring the availability of Cu and Zn in these calcareous soils.     

砂质土壤积累的铜和锌的可提取性与移动性研究

An investigation was conducted to study problems of determining a reasonable percentage for ecological water-use in the Haihe River Basin of China. Three key aspects for the ecological water requirement （EWR） were analyzed, involving i） the EWR for river system, ii） the EWR for wetlands and lakes, and iii） the EWR for discharge into the sea to maintain the estuary ecological balance of the Haihe River. The Montana method and related water level-flow relationships, and the statistic approach based on hydrological records were applied to estimate different components of EWR. The results showed that the total ecological water demand in the region, was about 3.47-14.56 billion m^3. Considering flow regime change and uncertainty, the ecological water demand could be estimated by the hydrological frequency approach. Preliminary analysis showed that for different annual runoff under the frequencies of 20%, 50%, 75% and 95%, the ecological water demand approached 12%-50%, 18%-74%, 24%-103%, 35%-148% and 16%-66%, respectively. By further analysis to balance ecological water-use and socioeconomic water-use, the rational percentage of ecological water-use was estimated as 35%-74%, that provides useful information to judge whether the allocation of water resources is reasonable, and was proved to be satisfactory by comparing with the practical condition.     

Micronutrients distribution in salt-affected soils of the Punjab in relation to soil properties

Salt-affected soils in arid and semi-arid tracts of the Indian Punjab are prone to deficiency of micronutrients. Nine profiles from alluvial terraces, sand dunes and palaeochannels in the southwestern Punjab were investigated for total and diethylenetriamine-penta-acetic acid (DTPA) extractable Zn, Cu, Mn and Fe. Soil physiography exerted significant influence on the spatial distribution of micronutrients. Total contents varied from 20–78 for Zn, 8–32 for Cu, and 88–466 mg kg^?1 for Mn and 0.82–2.53% for Fe. DTPA-extractable contents varied from 0.10–0.98 for Zn, 0.14–1.02 for Cu, 0.54–13.02 for Fe and 0.82–9.4 mg kg^?1 for Mn. Total contents were higher in fine-textured soil than in coarse-textured soils. Concentration of micronutrients in the surface layer was low and there occurred more accumulation in the Cambic horizon. Organic carbon, pH, clay, silt and calcium carbonate exerted strong influence on the distribution of micronutrients. DTPA extractable Zn, Cu, Mn and Fe increased with increasing organic carbon but decreased with increase in pH and calcium carbonate content. Total micronutrient contents increased with increase in clay, silt and calcium carbonate contents and decreased with increase in sand content.     

Distribution and Mobility of Trace Elements in Soils and Vegetation Around the Mining and Smelting Areas of Tharsis, Ríotinto and Huelva, Iberian Pyrite Belt, SW Spain

Trace elements may present an environmental hazard in the vicinity of mining and smelting activities. However, the factors controlling their distribution and transfer within the soil and vegetation systems are not always well defined. Total concentrations of up to 15,195 mg . kg ^–1 As, 6,690 mg . kg^–1 Cu, 24,820 mg . kg^–1 Pb and 9,810 mg . kg^–1 Zn in soils, and 62 mg . kg^–1 As, 1,765 mg . kg^–1 Cu, 280 mg . kg^–1 Pb and 3,460 mg . kg ^–1 Zn in vegetation were measured. However, unusually for smelters and mines of a similar size, the elevated trace element concentrations in soils were found to be restricted to the immediate vicinity of the mines and smelters (maximum 2–3 km). Parent material, prevailing wind direction, and soil physical and chemical characteristics were found to correlate poorly with the restricted trace element distributions in soils. Hypotheses are given for this unusual distribution: (1) the contaminated soils were removed by erosion or (2) mines and smelters released large heavy particles that could not have been transported long distances. Analyses of the accumulation of trace elements in vegetation (median ratios: As 0.06, Cu 0.19, Pb 0.54 and Zn 1.07) and the percentage of total trace elements being DTPA extractable in soils (median percentages: As 0.06%, Cu 15%, Pb 7% and Zn 4%) indicated higher relative trace element mobility in soils with low total concentrations than in soils with elevated concentrations.     

Influence of residual phosphate fertilizer on labile and extractable zinc in hawaii soils

Abstract

Zinc availability was studied using five soils from Hawaii which had histories of massive phosphorus applications. Heavy phosphate fertilization usually increased extractable Zn, irrespective of the extractant used. The extra extractable Zn associated with the added P probably came from Zn as an accessory element in the fertilizer. Treble superphosphate commonly used in Hawaii contains about 1400 ppm Zn. The Zn content of phosphate fertilizers must be considered before making statements about the effect of fertilizer P on Zn solubility and availability in soils.

Two solutions (0.1N HCl and 0.005M DTPA) were compared as Zn extractants for Hawaii soils. DTPA extracted less Zn than 0.1N HCl. Zinc extracted by repeated HCl treatment was more closely related to the labile Zn pool (E‐values and L‐values) than was DTPA‐extractable Zn. The results suggest that 0.1N HCl extractable Zn, Zn E‐value and Zn L‐value measured the quantity of a single fraction of soil Zn.

Repeated extraction of soil with 0.1N HCl seems to be a suitable procedure for evaluating the Zn status of acid, highly weathered soils of Hawaii.     

Diethylenetriaminepentaacetic Acid–Extractable Micronutrients Status in Soil under a Rice–Wheat System and Their Relationship with Soil Properties in Different Agroclimatic Zones of Indo‐Gangetic Plains of India

Available micronutrient status of zinc, copper, manganese, and iron (Zn, Cu, Mn, and Fe) in surface soil samples under a rice–wheat system collected from farmers' fields in 40 districts representing different agroclimatic zones of the Indo‐Gangetic Plain (IGP) were determined. The selection of farmers, villages, blocks, and districts within an agroclimatic zone was made on the basis of a multistage statistical approach. In Trans‐Gangetic Plains, the diethylenetriaminepentaacetic acid (DTPA)–extractable Zn ranged from 0.11 to 5.08, Cu ranged from 0.22 to 4.72, Mn ranged from 2.9 to 101.2, and Fe ranged from 1.05 to 97.9 mg kg^?1. In the Upper Gangetic Plains, the DTPA‐extractable Zn ranged from 0.04 to 2.53, Cu ranged from 0.06 to 4.32, Mn ranged from 11.1 to 421.0, and Fe ranged from 3.48 to 90.2 mg kg^?1. In the Middle Gangetic Plains, the DTPA‐extractable Zn ranged from 0.17 to 8.60, Cu ranged from 0.09 to 7.80, Mn ranged from 3.0 to 155.1, and Fe ranged from 9.22 to 256.7 mg kg^?1. In the Lower Gangetic Plains, the DTPA‐extractable Zn ranged from 0.04 to 3.46, Cu ranged from 0.21 to 4.38, Mn ranged from 9.54 to 252.2, and Fe ranged from 3.60 to 182.5 mg kg^?1. The intensively cultivated Trans‐Gangetic transect representing the midplain and Siwalik had more available micronutrients than the arid plain. Midplain and arid plain showed 17 to 20% of soil samples were low to medium in Zn and 5 and 8% were low in Fe. In the Upper Gangetic Plains, only 25% samples were deficient in Zn, especially in central and southwest plains. In the Middle Gangetic Plains, 20 to 30% of samples were deficient in Zn, and very few samples were deficient in other micronutrients. In the Lower Gangetic Plains, a majority of the samples were medium to high in micronutrients except in Barind and Rarh Plains where 30% of samples were deficient in Zn. In the Lower Gangetic Plains, the available micronutrients were plentiful. Available micronutrients increased with increase in organic C content and decreased with increase in sand content, pH, and calcium carbonate. These soils are alluvial in nature, and there was no definite pattern of micronutrient distribution with depth in the profile. However, there was more accumulation in the Ap horizon than in the B horizon.     

The profile distribution of total and extractable copper in selected Nigerian soils

Abstract

The profile distribution of total, DTPA‐ and 0.1N HCl‐extractable Cu was determined in 11 Nigerian soil profiles formed from various parent materials including the coastal plain sands, shales, basalt, granite and banded gneiss.

Total Cu ranged from 7 to 72 ppm with a mean of 35 ppm₀ The soils formed from basalt had the highest values while those on coastal plains had the least content. Generally, there was a higher content in the subsoils than in the surface horizons. The total Cu significantly correlated with percent clay and the free oxide contents of Fe and Mn.

DTPA ‐ and 0.1N HCl‐extractable Cu ranged from 0.08 to 2.81 ppm and 0.10 to 7.78 ppm, respectively. Soils on metamorphic rocks gave the highest values of DTPA‐extractable Cu. The DTPA‐extractable Cu ‐was only related to pH but the acid extractable Cu was associated with total Cu, clay, free Fe₂O₃ and MnO₂ contents.     

Relationship Between Extractable Metals in Acid Soils and Metals Taken Up by Tea Plants

Abstract

The accumulation of heavy metals in plants is related to concentrations andchemical fractions of the metals in soils. Understanding chemical fractions and availabilities of the metals in soils is necessary for management of the soils. In this study, the concentrations of copper (Cu), cadmium (Cd), lead (Pb), and zinc (Zn) in tea leaves were compared with the total and extractable contents of these heavy metals in 32 surface soil samples collected from different tea plantations in Zhejiang province, China. The five chemical fractions (exchangeable, carbonate‐bound, organic matter‐bound, oxides‐bound, and residual forms) of the metals in the soils were characterized. Five different extraction methods were also used to extract soil labile metals. Total heavy metal contents of the soils ranged from 17.0 to 84.0 mgCukg^?1, 0.03 to 1.09 mg Cd kg^?1, 3.43 to 31.2 mg Pb kg^?1, and 31.0 to 132.0 mg Zn kg^?1. The concentrations of exchangeable and carbonate‐bound fractions of the metals depended mainly on the pH, and those of organic matter‐bound, oxides‐bound, and residual forms of the metals were clearly controlled by their total concentrations in the soils. Extractable fractions may be preferable to total metal content as a predictor of bioconcentrations of the metals in both old and mature tea leaves. The metals in the tea leaves appeared to be mostly from the exchangeable fractions. The amount of available metals extracted by 0.01 mol L^?1 CaCl₂, NH₄OAc, and DTPA‐TEA is appropriate extractants for the prediction of metals uptake into tea plants. The results indicate that long‐term plantation of tea can cause sol acidification and elevated concentrations of bioavailable heavy metals in the soil and, hence, aggravate the risk of heavy metals to tea plants.     

Zinc and copper toxicity in peanut,soybean, rice,and corn in soil mixtures

Abstract

Applications of zinc (Zn) and copper (Cu) at excessive rates may result in phytotoxicity. Experiments were conducted with mixtures of soils that were similar except for their Zn and Cu levels. The critical toxicity levels (CTL) in the soils and plants for these elements were determined. Peanut (Arachis hypogaea L.), soybean [Glycine max (L.) Merr.], corn (Zea mays L.), and rice (Oryza sativa L.) were the crops grown. One soil mixture had Mehlich 3‐extractable Zn concentrations up to 300 mg dm^‐3 with no corresponding increase in soil Cu; two soil mixtures had soil Zn concentrations up to 400 and 800 mg dm^‐3 with a corresponding increase in soil Cu up to 20 and 25 mg dm^‐3, respectively; and four soil mixtures had no increase in soil Zn, but had Mehlich 1‐extractable Cu concentrations from 6 to 286 mg kg^‐1. Under a given set of greenhouse conditions, the estimated Mehlich 3‐extractable Zn CTL was 36 mg dm^‐3 for peanut, 70 mg dm^‐3 for soybean, between 160 and 320 mg dm^‐3 for rice, and >300 mg dm^‐3 for corn. No soil Cu CTL was apparent for peanut or soybean, but for corn it was 17 mg dm^‐3 and for rice 13 mg dm^‐3. With different greenhouse procedures and the Mehlich 1 extractant, the soil CTL for rice was only 4.4 mg kg^‐1. Therefore, peanut and soybean were more sensitive to Zn toxicity, whereas corn and rice were more sensitive to Cu toxicity. Plant Zn CTL for peanut was 230 mg kg^‐1, while that for soybean was 140 mg kg^‐1. Copper appeared to be toxic to corn and rice at plant concentrations exceeding 20 mg kg^‐1.     

Effect of incubation and microbial inhibition at field moisture capacity on changes in DTPA‐extractable Fe,Zn, and Cu in soils of varying pH

Abstract

To determine the effect of incubation on DTPA‐extractable Fe, Zn, and Cu in soils with a wide pH range (4.2 ‐ 9.4) and to determine the nature of this effect, soils were incubated at field moisture capacity for 1 week with and without a sterilant (toluene). After incubation these soils as well as their air‐dry counterparts were analyzed for DTPA‐extractable Fe, Zn, and Cu.

Incubated soils were significantly lower in DTPA‐extractable Fe, Zn, and Cu than air‐dry soils over all soil pH values tested but there was no significant difference in mean values for incubated soils due to the addition of toluene. The results suggest that, upon incubation at field moisture capacity, the decrease in DTPA‐extractable Fe, Zn, and Cu observed was noa‐microbial in nature.     

Metal accumulation by bermudagrass grown on four diverse soils amended with secondarily treated sewage effluent

Land treatment is increasingly being utilized as a method of waste disposal for both sewage effluent and sludges. While there has been considerable attention directed toward the fate of metallic constituents of sewage sludges, there have been fewer studies of the fate and mobility of metals appled to soils in sewage effluent. This study was undertaken utilizing secondarily treated sewage effluent amended to contain less than 1 mg l^?1 each of Cd, Cu, Ni, Pb, and Zn. The effluent was applied weekly for a period of 1 yr on large undisturbed monoliths of four diverse soils enclosed in lysimeters and sprigged to common bermudagrass (Cynodon dactylon L.). Soil samples were collected periodically and extracted with DTPA to measure plant available metals. Vegetation was harvested, weighed, subsampled and analyzed for total metal content. Total plant uptake of Cd, Cu, Pb, and Ni during the year was less than 1% of that applied. Vegetative uptake of Zn was as high as 2%. Metal uptake was greatest in the soil with the lowest initial pH. Heavy metal concentrations in plant tissue exhibited a cyclic trend. A similar increasing cyclic trend was evident in the DTPA extractable metals in the surface 0 to 12.5 cm of the treated soils. Decreases in plant and DTPA extractable metals occurred when the soils dried, allowing O₂ to enter. Vegetative concentrations of Cd, Cu, and Ni exceeded normal ranges of 0.2 to 0.8, 4 to 15, and 1.0 mg kg^?1, respectively, for vegetation while Pb and Zn were near normal. Only Cd concentration of vegetation posed a threat to grazing animals.     

Comparison of four extractants and chemical fractions for assessing available zinc and copper in soils of India

Abstract

Relative suitability of different extraction procedures for estimating available zinc (Zn) and copper (Cu) in soils was assessed using DTPA, 0.1 N HCl, ammonium acetate+EDTA, and double acid (HCl+ H₂SO₄) as extractants and rice as a test crop in Neubauer experiment. The relationships between Zn concentration and uptake of Zn by rice plants and Zn extracted by the different methods showed that DTPA‐TEA, pH 7.3, could very suitably be used to assess Zn availability in soils. However, 0.1 N HCl was better for assessing the Cu availability in soils to the rice plants. Water‐soluble and exchangeable fractions of Zn and Cu had significant positive correlations with Zn and Cu concentrations, respectively obtained by all the four extractants tested. The results also showed that DTPA and ammonium acetate+EDTA extracted organically bound Zn, whereas DTPA, 0.1 N HCl and ammonium acetate+EDTA extracted organically bound Cu. Water‐soluble, exchangeable and organic matter bound fractions exhibited significant relationships with Zn and Cu concentrations, their uptake and rice dry matter yield.