Use of a chlorophyll meter to evaluate the nitrogen status of dryland winter wheat

Abstract

Chlorophyll meter leaf readings were compared to grain yield, leaf N concentration and soil NH₄‐N plus NO₃‐N levels from N rate studies for dryland winter wheat Soil N tests and wheat leaf N concentrations have been taken in the spring at the late tillering stage (Feekes 5) to document a crop N deficiency and to make fertilizer N recommendations. The chlorophyll meter offers another possible technique to estimate crop N status and determine the need for additional N fertilizer. Results with the chlorophyll meter indicate a positive association between chlorophyll meter readings and grain yield, leaf N concentration and soil NH₄‐N plus NO₃‐N. Additional tests are needed to evaluate other factors such as differences among locations, cultivars, soil moisture and profile N status.     

Use of a hand‐held chlorophyll meter in winter wheat: Evaluation of different measuring positions on the leaves

A portable chlorophyll meter (Minolta SPAD‐502) was used to assess the nitrogen status of winter wheat (Triticum aestivum L.) in two fertilizer trials at Apelsvoll Research Centre, located in south‐east Norway. The midpoint of the last fully developed leaf was found to be the best position on the winter wheat plant on which to take chlorophyll meter readings. This conclusion was reached after examination of the relationships between soil‐plant analyses development (SPAD) readings taken at different positions on the plant and leaf nitrogen concentration, grain yield and grain protein content. Emphasis was also laid on finding a measuring position that was convenient from a practical point of view. The relationships between chlorophyll meter readings and the parameters investigated were better at Zadoks growth stage (GS) 49 than earlier in the season at GS 31.     

Chlorophyll meter as nitrogen management tool in malting barley

Abstract

Variable precipitation in many regions makes it difficult to predict yield goals and nitrogen (N) rates for malting grade barley (Hordeum vulgare L.). During years with below normal growing season precipitation, barley fertilized at the recommended rate often exhibits grain protein concentrations exceeding what is acceptable for malting. A study was conducted to evaluate the chlorophyll meter as a N management tool. Barley was grown under several N rates in the field. Chlorophyll meter readings and N additions were made at the Haun 4 to 5 growth stage, and grain yield and protein concentrations were evaluated at maturity. Chlorophyll meter readings, normalized as meter reading from treatment plot divided by that from a plot receiving a full N treatment at the Haun 4 to 5 growth stage, were correlated with grain yield (r²=0.67). Stands having normalized chlorophyll meter readings below 95% responded to N additions with yields equivalent to the fully fertilized stand and grain protein concentrations acceptable for malting. A N management strategy is proposed whereby 40 to 50% of the N calculated for the yield goal is applied at planting and a fully fertilized reference strip is included for each variety or soil type. At the Haun 4 to 5 growth stage, chlorophyll meter readings are taken in the reference strip and in the field. Normalized chlorophyll meter readings below 95% of the reference strip indicate a need for additional N fertilizer. This strategy will provide producers with additional time (up to a month) to evaluate growing season conditions before investing in additional crop inputs and will improve the likelihood that a barley crop acceptable for malting will be produced.     

Determination of cotton nitrogen status with a handheld chlorophyll meter

The ability of a hand‐held chlorophyll meter (SPAD‐502 Chlorophyll Meter³, Minolta Camera Co., Ltd., Japan) to determine the N status of cotton (Gossypium hirsutum L.) was studied at field sites in Alabama and Missouri. Meter readings on the uppermost fully‐expanded leaf were compared to leaf‐blade N and petiole NO₃‐N at first square, first bloom and midbloom as to their seed cotton yield predictive capability. Nitrogen was applied at rates of 0, 45, 90, 135, 180 and 225 kg ha^‐1 to establish a range of cotton chlorophyll levels, tissue N concentrations, and seed cotton yields. A typical curvilinear cotton yield response to N fertilizer was observed in Alabama experiments.

Because of adverse weather conditions, cotton yield in Missouri experiments did not respond to N. Chlorophyll meter readings were significantly correlated to leaf‐blade N concentration at all three stages of growth for all experiments. In Alabama, chlorophyll meter readings compared favorably to leaf‐blade N and petiole NO₃‐N with respect to their seed cotton yield predictive capability at all three stages of growth. It appears that hand‐held chlorophyll meters would be as reliable as leaf‐blade N and petiole NO₃‐N for predicting supplemental N fertilization requirements of cotton. However, more research will be required prior to use of chlorophyll meter readings for routine cotton‐N recommendation purposes.     

Chlorophyll meter readings in corn as affected by plant spacing

Abstract

Heightened environmental consciousness has increased the perceived need to improve nitrogen (N) use efficiency by crops. Synchronizing fertilizer N availability with maximum crop N uptake has been proposed as a way to improve N‐use efficiency and protect ground water quality. Chlorophyll meters (Minolta SPAD 502) have the potential to conveniently evaluate the N status of corn (Zea mays L.) and help improve N management. A potential problem with the use of chlorophyll meters is the effect of within‐row plant spacing on meter reading variability. Chlorophyll meter readings and leaf N concentration of irrigated corn at anthesis and grain yield at harvest were measured on plants grouped into eight within‐row plant spacing categories. Leaf N concentration was not affected by plant spacings, but chlorophyll meter readings and grain yield per plant increased as plant competition decreased and N fertilizer rate increased. These data indicate that avoiding plants having extreme spacings can greatly increase precision when using chlorophyll meters to evaluate the N status of corn.     

Chlorophyll Meter Readings in Winter Wheat: Cultivar Differences and Prediction of Grain Protein Content

Leaves of winter wheat (Triticum aestivum L.) were measured with a Hydro N-Tester (HNT), a portable chlorophyll meter, to obtain a relative estimate of leaf chlorophyll content, which gives an indication of plant N-status. The aim was to find if there were differences in HNT values between the winter wheat cultivars investigated, and to assess whether these values could be used for prediction of grain protein content (GPC). In Experiment 1, chlorophyll meter readings were taken at growth stages (GS) Zadoks 31 and 55 in five winter wheat (Triticum aestivum L.) cultivars in 17 annual field trials located in southeast Norway in 1996, 1997, and 1998. There were significant differences in HNT values between cultivars at both GS 31 and 55, but no differences were found between the three most commonly used cultivars in Norway at present, cvs. Bjørke, Mjølner and Rudolf. Because of the variation between years the HNT values at GS 55 were found to be unsuitable for direct prediction of the GPC. In Experiment 2, HNT was used in 21 annual N-fertilizer trials with winter wheat, performed in 1996 and 1997 and located in southeast Norway. A delay in the measurements from GS 55 to GS 65 generally improved the GPC prediction in 1996. Nitrogen applied at GS 55 increased GPC by approximately 1 unit of percent in cv. Folke, regardless of HNT value, while in cv. Portal nitrogen applied at this stage gave no increase in GPC at HNT values beyond 700.     

Chlorophyll meter predicts nitrogen status of tall fescue

The nitrogen (N) status of a crop can be used to predict yield and supplemental N fertilizer requirements, and rapid techniques for evaluating the N status of crops are needed. A study was conducted to determine the feasibility of using a hand held chlorophyll meter (SPAD 502, Minolta Co. Ltd., Japan) to monitor N status of tall fescue (Festuca arundinacea Schreb.). Four diverse tall fescue genotypes were grown at three locations in Alabama and fertilized at four N‐rates from 0 to 336 kg ha^‐1. A similar experiment was conducted in the greenhouse using soil from the same field sites. Chlorophyll meter readings (SPAD) were taken, and extractable chlorophyll content, tissue N concentration and dry matter yield were determined at harvest. SPAD, extractable chlorophyll content, tissue N concentration, and dry matter yield increased quadratically (0.67 < R² < 0.99) with increasing N fertilization in both experiments. All genotypes responded similarly to applied N, with some differences in magnitude. Relationships between SPAD meter readings and extractable chlorophyll and tissue N concentrations were linear with r² > 0.95. An additional independent variable, the square root of the inverse of SPAD, lowered the residual mean square by 11 and 16%, respectively, for tissue‐N and chlorophyll concentrations, but did not increase the R². This would be preferred for predictive purposes. Tissue N concentrations at higher N‐rates were sufficient for maximum yield which occurred at 290 and 248 kg N ha^‐1 for greenhouse and field, respectively, but were lower than previously reported sufficiency values. The chlorophyll meter is an easy and efficient method of detecting tall fescue N status.     

Optimum Nitrogen Fertilization of Winter Wheat Based on Color Digital Camera Images

Abstract

Site‐specific nitrogen (N) fertilizer management based on soil N_min (soil mineral N) and the plant N status (sap nitrate analysis and chlorophyll meter (SPAD) reading test) has been shown to be effective in decreasing excessive N inputs for winter wheat in the North China Plain, but the multiple sampling of soil and plants in individual fields is too time‐consuming and costly for producers and farmers. In this study, a color digital camera was used to capture wheat canopy images at a specific growth stage to assess N needs. Treatments included a farmer's N treatment (typical farmer practice), an optimum N treatment (N application based on soil–plant testing), and four treatments without N (one to four cropping seasons without any N fertilizer input). Digital images were analyzed to get red, green, and blue color‐band intensities for each treatment. Normalized intensities of the red, green, and blue color bands were well correlated with soil N_min, SPAD readings, sap nitrate concentration, and total N concentration of winter wheat. This research indicated the potential of using a digital camera as a tool combined with an improved N_min method to make N fertilizer recommendations for larger fields.     

Ammonium concentration in solution affects chlorophyll meter readings in tomato leaves

Abstract

Combinations of NH₄‐N:NO₃‐N usually result in higher tomato (Lycopersicon esculentum Mill.) yields than when either form of nitrogen (N) was used alone. Leaf chlorophyll content is closely related to leaf N content, but the effect of the NH₄‐N:NO₃‐N ratio on leaf greenness was not clear. The objective of this study was to determine the influence of NH₄‐N:NO₃‐N ratios on chlorophyll meter (SPAD) readings, and evaluate the meter as a N status estimator and tomato yield predictor in greenhouse production systems. Fruit yield and SPAD readings increased as the amount of NH₄‐N in solution increased up to 25%, while higher ratios of NH₄‐N resulted in a decline in both. The N concentration in tomato leaves increased as concentration of NH₄‐N in solution increased. Fruit yield increased as chlorophyll readings increased. SPAD readings, total N in leaves, fresh weight of shoots, and fruit yield all showed a quadratic response to NH₄‐N, reaching a peak at 25 or 50% of N as NH₄‐N. SPAD readings taken at the vegetative and flowering stages of growth had the highest correlation (r²=0.54) with N concentration in leaves, but this could not be used as a reliable estimate of N status and fruit yield. Lack of correspondence between high N concentration values and fruit yield indicated a detrimental effect of NH₄‐N on chlorophyll molecules or chloroplast structure. The SPAD readings, however, may be used to determine the optimum NH₄‐N concentration in solution to maximize fruit yield.     

Chlorophyll meter readings,N leaf concentration and their relationship with N use efficiency in oregano

Plant-based diagnostic techniques such as chlorophyll meter (CM) readings and nitrogen (N) leaf concentration are used to determine the level of crop N nutrition, but research is limited on perennial crops and especially on aromatic and medicinal plants such as Greek oregano (Origanum vulgare spp. hirtum). The objectives of this study were to determine in a perennial crop species whether there is a relationship between the CM readings, N leaf concentration, and N use efficiency (NUE) and to use CM readings as a diagnostic tool for predicting dry matter yield in response to N fertilization. The CM readings varied across environments, growth stages, and fertilization treatments and were correlated with NUE. NUE was also affected by the environment, N fertilization, and interactions among these variables. This study provides new information about the effect of N application on CM readings, N leaf concentration, and NUE in a perennial crop such as oregano.     

Using a chlorophyll meter to manage nitrogen applications to corn with high nitrate irrigation water

Abstract

Chlorophyll meters can aid in measuring the nitrogen (N) status of corn (Zea mays), but will the use of chlorophyll meters decrease total N use or increase corn grain yield? Use of a fully fertilized reference strip with a chlorophyll meter (SPAD 502) is an accepted management strategy. The critical level of relative chlorophyll necessary to trigger supplement N is uncertain. To determine the impact of using a 96 or 92% critical level of chlorophyll readings relative to a fully fertilized reference strip, 0 and 112 N kg ha^‐1 were applied at planting for three years. Specific plots were fertilized with an additional 56 kg N ha^‐1 whenever the meter reading on those plots was below either 96 or 92% of the reference area. The experiment was conducted on two Typic Haplustolls and an Entic Haplustoll. Irrigation water contained nitrate ranging from 20 to 38 mg L^‐1. Irrigation water supplied from 43 to 120 kg N ha^‐1 season^‐1. For all treatments that received N‐supplements based on the chlorophyll meter, yields were statistically the same. The chlorophyll meter is a useful management tool to help schedule N applications, when irrigation water is contaminated with nitrate since use of the chlorophyll meter prevented any yield limiting N deficiencies.     

SOIL-CROP NITROGEN RELATIONSHIPS IN MAIZE GROWN ON CALCAREOUS FIELDS

Maize yield is mainly controlled by nitrogen (N) fertilizers. Failure to predict economic optimum N rate causes over-fertilization of N through traditional application methods. Five field trials were conducted to study soil-crop N relationships in maize under field conditions. Optimum level of N application in this study was much lower than conventional N fertilization in calcareous soils of southern Iran, demonstrating over-fertilization of N fertilizers by farmers. Linear regression equations were found between total nitrogen (TN) and chlorophyll meter readings (CMR) (years 2001 and 2002) in both locations demonstrating that chlorophyll meter might be used in predicting TN in maize shoot. Results indicated that soil sampling location influenced on soil NO₃-N concentration at 4–6 leaf stage. In conclusion, N use efficiency can be improved by in-season soil NO₃-N testing. It appears that in Kooshkak and Bajgah experimental stations, soil samples for NO₃-N testing should be collected from shoulder and bottom of furrows, respectively.     

华北地区采用无机氮测试和植株速测进行夏玉米氮肥推荐

A field experiment with a split-plot design was carried out at Dongbeiwang Farm in Beijing Municipality to establish reliable N fertilizer recommendation indices for summer maize (Zea mays L.) in northern China using the soil Nmin(mineral N) test as well as the plant nitrate and SPAD (portable chlorophyll meter readings) tests. The results showed that Nrnin sollwert (NS) 60 kg N ha^-1 at the third leaf stage and N rate of 40 to 120 kg N ha^-1 at the tenth leaf stage could meet the N requirement of summer maize with a target yield of 5.5-6 t ha^-1. Sap nitrate concentrations and SPAD chlorophyll meter readings in the latest expanded maize leaves at the tenth leaf stage were positively correlated with NS levels, indicating that plant nitrate and SPAD tests reflected the N nutritional status of maize well. Considering that winter wheat subsequently utilized N after the summer maize harvest, the 0-90 cm soil Nmin (74 kg N ha^-1) and apparent N loss (12 kg N ha^-1) in the NS60＋40 treatment were controlled at environmentally acceptable levels. Therefore NS60＋40, giving a total N supply of 100 kg N ha^-1, was considered the optimal N fertilizer input for summer maize under these experimental conditions.     

Optical Sensor‐Based Algorithm for Crop Nitrogen Fertilization

Abstract

Nitrogen (N) fertilization for cereal crop production does not follow any kind of generalized methodology that guarantees maximum nitrogen use efficiency (NUE). The objective of this work was to amalgamate some of the current concepts for N management in cereal production into an applied algorithm. This work at Oklahoma State University from 1992 to present has focused primarily on the use of optical sensors in red and near infrared bands for predicting yield, and using that information in an algorithm to estimate fertilizer requirements. The current algorithm, “WheatN.1.0,” may be separated into several discreet components: 1) mid‐season prediction of grain yield, determined by dividing the normalized difference vegetative index (NDVI) by the number of days from planting to sensing (estimate of biomass produced per day on the specific date when sensor readings are collected); 2) estimating temporally dependent responsiveness to applied N by placing non‐N‐limiting strips in production fields each year, and comparing these to the farmer practice (response index); and 3) determining the spatial variability within each 0.4 m² area using the coefficient of variation (CV) from NDVI readings. These components are then integrated into a functional algorithm to estimate application rate whereby N removal is estimated based on the predicted yield potential for each 0.4 m² area and adjusted for the seasonally dependent responsiveness to applied N. This work shows that yield potential prediction equations for winter wheat can be reliably established with only 2 years of field data. Furthermore, basing mid‐season N fertilizer rates on predicted yield potential and a response index can increase NUE by over 15% in winter wheat when compared to conventional methods. Using our optical sensor‐based algorithm that employs yield prediction and N responsiveness by location (0.4 m² resolution) can increase yields and decrease environmental contamination due to excessive N fertilization.     

Utilizing Existing Sensor Technology to Predict Spring Wheat Grain Nitrogen Concentration

Optimum grain nitrogen (N) concentration and yield in spring wheat (Triticum aestivum L.) can be problematic without proper N fertilizer management. Sensor-based technologies have been used for application of fertilizers and also to predict yield in wheat, although little has been done in the prediction of grain N. Field studies were conducted in South Dakota in 2006 (Gettysburg, Bath, and Cresbard) and 2007 (Gettysburg, Aurora, Leola, and Artas). There were five N treatments (0, 56, 112, 168, and 224 kg N ha^?1) applied pre-plant with a second N application applied foliar at anthesis. Sensor readings were taken at growth stages Feekes 10, anthesis, and postfoliar application using the GreenSeeker Hand Held optical sensor. Grain samples were taken at maturity and analyzed for total N. Using similar information collected in 2003 and 2005, a critical normalized difference vegetation index (NDVI) value was determined using the Cate–Nelson procedure. The critical NDVI value needed to ensure optimum grain N was 0.70. In 2006 and 2007, the plots that received an application of N at anthesis had higher grain N than the plots not receiving N. There was also a significant response between applied N and grain yield. The results show that with further studies, the Greenseeker could be used to apply N to maximize yield and grain N in a precise and accurate manner.     

Leaf chlorophyll readings as an indicator of nitrogen status and yield of spinach (Spinacia oleracea L.) grown in soils amended with Luecaena leucocephala prunings

A field study was conducted to evaluate the nitrogen status and yield of spinach grown in soils amended with prunings of Leucaena leucocephala, (applied at a rate of 3, 5, 7 or 11 t ha^?1). A ‘no fertilizer’ 0 nitrogen (N) and 150 kg N ha^?1 (recommended) were the control treatments. SPAD readings were recorded for the top six leaves. Nitrogen sufficiency indices were used to indicate the N status of plants. Application of L. leucocephala prunings increased spinach yields (8.98–13.86 t DM ha^?1) relative to the 0N treatment (1.35 t DM ha^?1) and yields increased with increasing rate of pruning application. SPAD readings showed a linear increase with the increase in applied prunings. There was preferential distribution of N to upper leaves. The relationship between shoot N concentration and SPAD readings was linear and strongest for the top three leaves (r² = 0.84–0.92). The results indicate the potential of chlorophyll meter readings in assessing N status of leafy vegetables grown on soils amended with different levels of legume tree prunings.     

Evaluation of the Minolta SPAD‐502 chlorophyll meter for nitrogen management in corn

The field study investigated the relationship of Minolta SPAD 502 (SPAD) readings to applied nitrogen (N) fertilizer rate, corn (Zea mays L.) yield, and leaf N concentration. The experiment was conducted on a total of six sites in Illinois during 1991 and 1992. Ten different open pedigree corn hybrids were grown at a final population of 65,000 plants ha^‐1. Nitrogen was applied at four rates(0, 90, 180, and 270 kgN ha^‐1) as 28% liquid N solution. Significant main effects of environment (E), and hybrid (H), and E x H interaction were detected for all measured parameters. SPAD readings and leaf N concentration at all sampling times (V7, R1, and R4) as well as grain N concentration were affected by N fertilizer rate. Maximum mean grain yield and maximum grain N concentration were obtained at 110 and 195 kg N ha^‐1, respectively. At all sampling times the correlation of SPAD readings to N fertilizer rate were low but significant (R=0.22 at V7 and R1, R=0.11 at R4). SPAD correlation to corresponding leaf N concentration improved over time. The Pearson correlation was R=0.33 at V7 and increased to R=0.78 at R4. The SPAD meter did a good job at providing a measure of the relative greenness of living leaves at a specific point in time. Chlorophyll readings can therefore be useful in detecting N deficiencies in growing crops. But, the SPAD meter cannot be used to make accurate predictions of how much fertilizer N will be needed by a crop during the future growing season. We conclude then that the SPAD meter will be most useful as a diagnostic aid rather than a tool for N management in corn.     

Agronomic Assessment of Nitrogen Use Efficiency in Spring Wheat and Interrelations with Leaf Greenness Under Field Conditions

Four spring wheat genotypes (Triticum aestivum L.) were grown without (N0 = 0 kg N ha^?1) and under ample (N1 = 250 kg ha^?1) nitrogen (N) fertilizer in field experiments in two seasons. The aim was to assess genotypic variation in N use efficiency (NUE) components and N-related indices during grain filling thus to identify superior wheat genotypes. Leaf chlorophyll (SPAD) readings at crucial growth stages were employed to help differentiate genotypes. Interrelations between yield and N-related indices with SPAD, where also assessed to explain possible pathways of improving NUE early in the growing season. Results showed that genotypic effects on NUE were mostly evident in 2000, a year with drier preanthesis and wetter postanthesis than the normal periods. ‘Toronit’ almost always had the highest biomass yield (BY) and grain yield (GY). Except in 1999 under N0, ‘L94491? showed the highest % grain N concentration (GNC). Genotypes affected SPAD at almost all stages and N fertilization delayed leaf senescence for all genotypes and growth seasons. Correlations between SPAD at different growth stages and GY, N biomass yield at maturity (NBYM) and GNC were significant (P≤ 0.001), positive and strong/very strong (>r = 0.7). N translocation efficiency (NTE) was inversely related to PANU (~r = ? 0.77, P≤ 0.001), suggesting that N after anthesis is being preferentially transported to the ears to meet the N demand of the growing grains. It is concluded that there is still a large potential for increased NUE by improved N recirculation, use of fast and inexpensive crop N monitoring tools and high yielding, N uptake efficient genotypes.

Abbreviations: NUE, Nitrogen use efficiency; SPAD, Minolta SPAD-502 chlorophyll meter, NHI, nitrogen harvest index; HI, Harvest index; NTE, N translocation efficiency from vegetative plant parts to grain; DMTE, dry matter translocation efficiency; CPAY, contribution of pre-anthesis assimilates to yield; PANU, Post-anthesis N uptake, d.a.s., days after sowing, N0, zero (0) kg ha^?1 applied N fertilizer, N1, 250 kg ha^?1 applied N fertilizer.     

INTER-RELATIONSHIPS OF APPLIED NITROGEN,SPAD, AND YIELD OF LEAFY AND NON-LEAFY MAIZE GENOTYPES

The SPAD chlorophyll meter was found to be a reliable, quick, and non-destructive tool used for directly measuring leaf chlorophyll and indirectly assessing the proportional parameter of leaf, and by extension, plant nitrogen (N) status. The meter has been used successfully to assess leaf N in conventional maize crops, but it has not been used with new maize (Zea mays L.) genotypes containing leafy (L) and reduced stature (RS) traits. SPAD meter readings were collected on the uppermost fully developed leaves (before silking) and on the ear leaf (after silking) of field grown maize genotypes with and without the L and RS traits. The experiment was conducted during 1996 and 1997 at two sites in Eastern Canada (Ottawa and Montreal). At each site in each year, a split plot arrangement of two treatment factors was used in a randomized complete block design with four blocks. The main plot treatments were levels of N (0, 85, 170, and 255 kg ha^?1), with six maize genotypes as subplot treatments. The hybrids included: (i) leafy reduced-stature, LRS, (ii) non-leafy normal stature, NLNS, (iii) leafy normal stature, (LNS), (iv) non-leafy reduced-stature, NLRS, (v) conventional commercial hybrids, Pioneer 3905 as the hybrid check for late maturity, and (vi) Pioneer 3979, a check for early maturity. The hybrids were chosen on the basis of their contrasting canopies and root architecture. The SPAD meter readings were collected on the same five plant genotypes over time (six times per site per year, except four times for the Ottawa site in 1997). All genotypes showed increasing meter reading values as plants aged until silking. In general, SPAD meter readings increased as N fertilization level increased at each measurement date for both sites and years. In general, LNS and P3905 hybrids showed greater SPAD meter readings than other hybrids at all sampling dates for both sites and growing seasons. Applied N rates were significantly correlated with the SPAD meter readings. More highly significant relationships were found for N fertilizer levels and SPAD meter readings for the hybrids in 1997 than for the hybrids in 1996. For the Montreal site in 1997, LRS, LNS and P3905 hybrids were among those showing the highest r values between N level and SPAD readings. The correlation coefficients between SPAD readings and grain yield were generally lower. However, the NLNS hybrid had a high SPAD-yield correlation at the Macdonald site in 1997.     

Assessing Variations in SPAD‐502 Chlorophyll Meter Measurements and Their Relationships with Nutrient Content of Trembling Aspen Foliage

Abstract

The relative chlorophyll content in leaves estimated by the SPAD‐502 chlorophyll meter can be an efficient way to evaluate plant nitrogen (N) status in many crops and some tree species. In this study, the SPAD meter was used to look for relationships between relative leaf chlorophyll content and leaf nutrient concentration in trembling aspen. The variations in leaf chlorophyll measurements were also evaluated by using three sampling times and three measurement techniques. Hypothetical experimental designs were created to achieve better design efficiency. The best representation of overall leaf chlorophyll was found when six SPAD readings were taken at different locations on each leaf. There was a positive correlation between overall leaf N and estimated chlorophyll content, especially in the top part of the trees. Overall, this study suggested that the SPAD meter can give an estimation of trembling aspen nutritional status, especially if the differential partitioning of N within trees is considered.