Comparison of phosphate‐phosphorus and total phosphorus in DTPA extracts for assessing plant‐available phosphorus in soilless potting media

Abstract

Hakea francisiana and H. laurina were grown in soilless media based on pine bark, to which had been added one of the following phosphorus (P) sources: crushed bone, rock phosphate, calcined rock phosphate, sewage sludge, or sludge compost. Available P was assessed through extraction with unbuffered 2 mM DTPA. Similar regression equations between shoot P content and P in 2 mM DTPA extracts of the media at potting were obtained for both total P in the extract (determined by inductively coupled plasma emission spectrometry) and PO₄‐P. The difference between them was small compared with the variation caused by different rates of dissolution of P the various sources during the growing period. Extractants give only an approximate guide to plant P uptake when the medium contains sources that slowly dissolve during the growing period. Nevertheless, the data indicate that, irrespective of P source, the maximum P concentration in a 2 mM DTPA extract (1:1.5 v/v) of the medium that is tolerated by P‐sensitive plants is 3–4 mg/L. This is similar to the concentration found previously for superphosphate as the source of P.     

Semi‐micro determination of total phosphorus in soils,sediments, and organic materials: A simplified perchloric acid digestion procedure

Abstract

A semi‐micro modification of perchloric acid digestion method for the determination of total P in soils, sediments, and organic materials is described. Samples are digested for 30 minutes with nitric acid at 115 °C and then for 2 hours with perchloric acid at 170 °C in an aluminum heating block. After dilution, samples are hydrolyzed at 100 °C for 1 hour in order to decompose pyrophosphate, and the liberated orthophosphate is determined without a neutralization step by the phosphomolybdate blue method of Murphy and Riley (1962). Comparability with the method of Sommers and Nelson (1972) is demonstrated using nine samples of different types. The same reaction conditions of digestion and color development as in the determination of total P in waters (Kopá?ek and Hejzlar, 1993) enable a comparable estimate of total P in solid and water samples in complex environmental studies.     

A phenol‐hypochlorite manual determination of ammonium‐nitrogen in Kjeldahl digests of plant tissue

Abstract

A phenol‐hypochlorite (Berthelot) procedure for determining NH₄‐N in acid digests of plant tissue is described. EDTA suppresses Cu interference and precipitation of hydroxides and phosphates. The oxidizing reagent includes an alkaline buffer to account for variations in acidity between digestion solutions, the main source of error in an unbuffered procedure. The reagent combinations and addition sequence employed allow for delays of up to at least 30 minutes between reagent additions without affecting the final colour intensity. The proposed procedure is as sensitive as similar methods used for determining NH₄‐N in natural waters and yields results which agree well with those obtained by steam distillation.     

A preliminary comparison of the ammonium acetate‐edta soil phosphorus extraction method to the bray‐1 and olsen soil phosphorus extraction methods

Abstract

The ammonium acetate (NH₄OAc)‐EDTA soil phosphorus (P) extraction method was compared to either the Bray‐1 soil P extraction method for non‐calcareous soils or the Olsen soil P extraction method for calcareous soils to predict com and wheat plant tissue P concentration and grain yield responses. The NH₄OAc‐EDTA method predicted yield and tissue P concentration responses to P fertilizer applications more accurately than the Olsen method at three of five sites. Both the Bray‐1 and NH₄OAc‐EDTA methods were successful in predicting corn and wheat yield responses to P fertilizer applications in non‐ calcareous soils in many locations. However, a direct comparison of extracted soil P levels showed that the NH₄OAc‐EDTA method extracted soil P at levels which were more closely related to the Bray‐1 method than the Olsen method.     

A method for estimating organic‐bound iron and aluminum contents in soils

Abstract

Estimation of organic‐bound iron (Fe) and aluminum (Al) is an important diagnostic technique in pedology. The commonly used sodium pyro‐phosphate method yields somewhat ambiguous results and is inconvenient if an ultracentrifuge is not available in the laboratory. This study showed that 0.1M sodium nitrilotriacetate (NTA) could be used instead of pyrophosphate. The Fe and Al extracted by 0.1 M NTA were highly correlated to that by sodium pyrophosphate with R² = 0.993 for Fe and R² = 0.992 for Al, while the dissolution effects on standard mineral samples in NTA was kept at a minimum. NTA has the advantage of not requiring ultrafiltration, ultracentrifugation, or the addition of a flocculating agent as is the case for the pyrophosphate method.     

Effects of phosphorus application method and rate on furrow‐irrigated ridge‐tilled grain sorghum

Conservation tillage systems, including ridge‐tillage, have become increasing popular with producers in the central Great Plains because of their effectiveness in controlling soil erosion and conserving water. A major disadvantage of the ridge system is that nutrient placement options are limited by lack of any primary tillage options. The objective of this research was to investigate the effects of method of phosphorus (P) placement and rate on irrigated grain sorghum [Sorghum bicolor (L.) Moench] grown in a ridge‐tillage system on a soil low in available P. This experiment was conducted from 1993 to 1995 on a producer's field near the North Central Kansas Experiment Field at Scandia, Kansas on a Carr sandy loam soil (course, loamy, mixed, calcareous, mesic, Typic Udifuvents). Treatments consisted of fertilizer application methods, surface broadcast, single band starter (5 cm to the side and 5 cm below seed), dual band starter (one band on each side of the row), and knifed in the center of the row middle (38 cm from each adjacent row). Each of these treatments was made at either 22 or 44 kg P₂O₅ ha^‐1, and nitrogen (N) also was included at the rate of 13 kg ha^‐1. Additional treatments were, a combination of 13 kg N and 44 kg P₂O₅ ha^‐1 applied half broadcast and half as a single band starter, a 1:1 N:P₂O₅ ratio (44 kg N and 44 kg P₂O₅ ha^‐1) applied as a single band starter, and a 3:1 ratio (134 kg N and 44 kg P₂O₅ ha^‐1) applied as a single band starter. A no‐P check plot also was included. Broadcast and center‐of‐row middle knife applications were made approximately 1 week before planting. After planting, N was balanced on all plots to give a total of 180 kg ha^‐1. Applied P treatments improved grain yield and nutrient uptake and consistently shortened the time from emergence to mid‐bloom in all 3 years of the experiment. On this low soil test P soil, treatments that subsurface banded P increased grain yield by 1.27 Mg ha^‐1 compared to broadcast treatments. Placing N and P in a single starter band 5 cm to the side and 5 cm below the seed was as effective as placing a band on each side of the row. Knife applying N and P in the center of the row was not as effective as placement beside the row. Single band starter application of N and P in a 1:1 and or 3:1 N:P₂O₅ ratio consistently increased yields and nutrient uptake and shortened the time to mid‐bloom as compared to the single band starter treatment that provided only 13 kg N ha^‐1. Over the 3 years of the study, these 1:1 and 3:1 N:P₂O₅ ratio starters were clearly superior to an other treatments.     

A copper‐phosphorus interaction in wheat 1

Abstract

Examination of results from two experiments designed to compare copper (Cu) carriers, rates, and application methods and an experiment designed to test the residual effects of Cu on spring wheat (Triticum aestivum L.) pointed to the existence of an interaction between Cu and phosphorus (P). Mineral element data for aboveground whole‐plant samples from the three experiments indicated that, with low Cu levels in the soil, when Cu was applied as CUSO₄ in an apparently unavailable position, or when the Cu had been applied to the soil as CuHEDTA, P concentrations in the plant were very high. Also, when CUSO₄ was applied to the soil and incorporated to a depth of 60 to 100 mm, concentrations of Cu increased and P decreased in the plant. In each case, if Cu concentrations in the plant were adequate, then normal plant development occurred. A Cu‐P interaction which affected P uptake or translocation was evident.     

Variation of plant‐test phosphorus for individual lupin and wheat tops

Abstract

Two field experiments measured variation of phosphorus (P) concentration in dried tops (plant‐test P) of individual lupin (Lupinus angustifolius cv. Merrit) and wheat (Triticum aestivum cv. Cadoux) plants collected from random locations in experimental plots treated with different levels of superphosphate. Plant‐test P for the individual tops varied by between 4 to 65% of mean values. Coefficient of variation for the 10 individual plants per plot varied from 10 to 24%. For each plot, mean plant‐test P for the 10 individual tops were similar to values for bulk samples of 30 plants collected at random within the plot. It is concluded that a bulk sample of 30 lupin or wheat plant tops need to be collected from uniform areas in farmer paddocks to provide a representative estimated of the current P status of the crop.     

Use of a solid‐state chloride electrode for chloride determinations in soil extracts

Abstract

Studies were conducted to evaluate the use of a solid‐state chloride electrode for chloride determinations in soil extracts. The solid‐state chloride ‐electrode was used as an end point detector in the titration of chloride with AgN0₃ and direct reading of chloride in solution. The solid‐state electrode gave results very similar to those obtained with a silver electrode (r = 0.999) when used as an end point detector. Values obtained using the solid‐state electrode as a direct reading electrode were also well correlated (r = 0.998) but were slightly higher than results obtained by titration with a silver electrode.     

Importance of acid‐insoluble residue in plant analysis for total macro and micro elements

Abstract

Insoluble siliceous residues remaining after HNO₃/HClO₄ decomposition of the plant tissues National Bureau of Standards, standard reference materials spinach (Spinacea oleracea), orchard leaves and tomato leaves (Lycopersicon escuientum), contained varying quantities of the macro and micro elements Na, K, Mg, Ca, Mn, Fe, Cu and Zn. For the different samples with total element concentrations ranging from 11 μg/g (Cu in orchard leaves) to 45,000 μg/g (K in tomato leaves), residues contained element concentrations ranging from 0.05 to 88 times the respective concentrations in the samples. Contributions of residues to element concentrations in the plant tissues varied from 0.04 to 42% of the total concentrations. Overall, these constituted negligible (ca 0.1% for Mg and Ca), small (0.5% for Zn, Mn, K and 1% for Cu), and large (6% for Fe, 28% for Na) contributions depending on sample and analyte. Residue contributions to total element concentrations of plant tissues must be considered for reliable estimations of macro and micro elements.     

A computer‐assisted method for CEC estimation and quality control in a routine soil‐test operation

Abstract

Cation‐exchange capacity (CEC) of 30 Alabama soils was estimated by two different methods based or routine soil‐test results consisting of soil‐water pH, Adams‐Evans buffer pH, and Mehlich‐1 extractable cations (K, Mg, and Ca), which were obtained automatically by a computerized data acquisition system. In one method, CEC was calculated by solving a quadratic equation involving soil‐water and buffer pH's; in the other, CEC was estimated as the summation of extractable cations and exchangeable acidity. The two estimated CEC's agreed well with each other and also had the same magnitude as CEC determined by the normal NaOAc, pH 8.2 method. By averaging the two calculated values, an even closer estimation of the measured CEC was found. These calculations and comparisons can be accomplished quickly and efficiently by a minicomputer via a simple FORTRAN program.

In addition, a discrepancy between the two estimated CEC's would indicate possible errors in analytical determinations and/or the inadequacy of the soil testing procedures. Therefore, an additional means for quality control in a routine soil‐test operation can be obtained by comparing the two CEC values.     

Influence of chloride on the uptake and trans‐location of phosphorus in potato

Both chloride (Cl) and sulphate (SO₄) were found to impair the uptake of phosphorus (H2³²PO₄) through the roots of potato in a hydroponically conducted experiment. Radioactive phosphorus (³²P) was used as the marker. The presence of either Cl and SO₄ alone in the nutrient solution made no difference on the impairment. Both ions also inhibited the long‐distance transport of P from roots to shoots. The inhibition by Cl was much greater than that by SO₄, and increased with the concentration of Cl rising from 0 to 15.5 mM in solution.     

Colorimetric determination of phosphorus in citrate‐bicarbonate‐dithionite extracts of soils

Abstract

Existing colorimetric methods for determining phosphorus (P) in citrate‐bicarbonate‐dithionite (CBD) extracts from soils and sediments require pretreatments of extracts prior to the determination of the element. A method is proposed for the determination of P in CBD extracts without any chemical treatments. Maintaining the citrate concentration below 2.4 mM, maximum and stable absorbance develops in P‐Mo‐Sb system within one hour and remains stable for 20 hours. An evaluation of potential interference was made by adding varying amounts of P to CBD extracts over a range of extractable iron (Fe) and aluminum (Al) levels. Near 100 percent recovery of the added P suggests the applicability of the method to soils and sediments. Major advantages of the method are speed, simplicity, reliability and stability of the colored complex.     

A new soil test method for the determination of plant‐available cadmium in soils

Abstract

A new soil test procedure using 1M NH₄Cl was developed for the extraction of plant‐available cadmium (Cd) from soils. Five grams of soil is weighed into a 50‐mL polyethylene vial to which 30 mL of 1M NH₄Cl solution is added. The soil suspension is then shaken on a horizontal shaker for 16 h at 25°C at 180 cycles per min. The suspension is then centrifuged at 2,500g for 5 min and the supernatant filtered through a 0.45 μm nitrocellulose filter under vacuum. Cadmium in the extract is then determined at 228.8 nm on a graphite furnace equipped atomic absorption spectrophotometer. A highly significant correlation was observed between the natural logarithm (In) of 1M NH₄Cl‐extractable Cd in soils and the Cd content in the grain of durum wheat (Triticum turgidum var. durutn L.) grown on the same soils (r = 0.974, p = 3.8 x 10^‐7). In comparison with several commonly used extradants, such as ABDTPA, CaCl₂, NH₄OAc, and NH₄NO₃, the 1M NH₄Cl‐extracted Cd from soils was found to be a better index of Cd availability.     

Short‐term evolution of phosphorus in an ultisol

Abstract

Evolution of residual phosphate was monitored as function of time on an Ultisol without cropping in a field trial at Sembawa Rubber Research Station, South Sumatra, Indonesia. Three treatments were imposed and either triple superphosphate at a rate of 600 kg ha^‐1 containing 21.54% phosphorus (P) or lime at the rate of 4.14 tons ha^‐1 was applied. During the 20 months of incubation, extractable phosphate as evaluated by hydrochloric acid‐ammonium fluoride (Bray‐I) declined in all treatments. The decrease in the P fertilized plot was more pronounced in relation to the control and the limed soil. Only 56% of P added to the soil was initially recovered as extractable P. This percentage was 30–40% in limed soils. No significant pH difference was recorded for all treated plots except for limed soils. An increase of 1.3 pH units was initially obtained by liming, but the pH dropped to about 0.9 unit after 20 months of incubation. In addition, total P in arable layer remained fairly constant. According to the recoveries of P added referring to 4.3 P 100 g^‐1, half‐life of a single P dose was estimated at 31 months for a P fertilized soil and almost threefold delay for combination of P fertilization and lime application, which was obtained from the following equation:     

Evaluation of methods for determining sulphur‐35 and sulphur‐32 in the same trapping solution of Johnson and Nishita method

Abstract

A laboratory study was conducted to investigate whether the Zn‐Na acetate solution, used for trapping H₂S in the Johnson‐Nishita method (1952), could be used to quantitatively determine ³⁵S and ³²S in the same trapping solution, as the Zn‐Na acetate solution allows more sensitive S determination using methylene blue than does the bismuth sulphide colour development of H₂S trapped in NaOH solution. Results obtained using the Zn‐Na acetate solution were variable compared with those obtained from the normal procedure of using NaOH as the H₂S trapping solution, indicating that the Zn‐Na acetate solution was unsuitable, probably because of the rapid transformation of H₂S to water‐insoluble ZnS.

The applicability of methylene blue development for H₂S trapped in NaOH solution was also investigated. Results showed that ³²S could be determined quantitatively provided methylene blue colour development was conducted less than 30 minutes after the removal of H₂S‐trapping NaOH solution from the Johnson‐Nishita digestion‐distillation unit. A delay of more than 30 minutes caused a significant linear reduction (R² = 0.86 ‐ 0.92***) in methylene blue colour, indicating that H₂S in NaOH solution was readity: oxidized to other S forms which did not undergo methylene blue development.

Toluene and dioxane were compared as liquid scintillants for assaying ³⁵S activity trapped as H₂S in either NaOH or Zn‐Na acetate solution before and after methylene blue development. Before colour development, toluene improved the counting efficiency of ³⁵S in NaOH solution by 23 to 27%. After colour development, toluene improved the counting efficiency of both NaOH and Zn‐Na acetate solutions.     

A simple method for determination of ammonium in semimicro‐Kjeldahl analysis of soils and plant materials using a block digester

Abstract

A simple method for determination of ammonium in semimicro‐Kjeldahl analysis of soils and plant materials using a Tecator or Technicon 40‐tube block digester is described. It involves use of an inexpensive steam distillation apparatus that permits direct distillation of ammonium from the tubes used for Kjeldahl digestion in 40‐tube block digesters. The method is rapid and precise, and it gives results that agree closely with those obtained by the customary method of ammonium N analysis involving transfer of the Kjeldahl digest before distillation.     

A graphical method for fertilizer sharing in a limited‐capital situation

Abstract

When fertilization capital is limited, the maximum economic rate which gives the greatest profit per unit area cannot be applied to all crops, therefore, a predicting method for the most profitable fertilizer rates for each crop is needed. This work describes a graphical method for sharing the available fertilizer between two different crops, or else the same crop in two different soils or crop sequences where the fertilizer needs are not the same. The method can also be used in the case of irrigation when the capital or the amount of the available water are limited.     

A turbidimetric method for determining phosphate‐extractable sulfates in tropical soils

Abstract

Turbidimetric methods, using Ba ions to precipitate SO₄, are frequently used to determine soil sulfates extracted with phosphate solutions. These methods, as routinely performed, seriously underestimate SO₄ in some soils of the tropics because phosphate is removed from the extractant by soil adsorption and because many extracts fail to yield satisfactory precipitate even if the extracting procedure is adequate. Decolorizing the extracts with carbon black, treating extracts with strong oxidizing agents, adding SO₄ spikes, and seeding the extracts with BaCl₂ seed‐crystals improve precision, but some extracts, especially those from soils derived from volcanic ash, do not yield reliable precipitates even though these procedures are employed. This paper presents a method that consistanlty yielded more SO₄ than other turbidimetric procedures with which it was compared. The proposed method was further validated against an ion‐chromatographic method for SO₄ determination. The two methods yielded virtually identical results.

The proposed method consists of extracting SO₄ with 0.04 M Ca(H₂PO₄)₂ pH 4, at a soil‐to‐solution ratio of 1: 10. Repeated extraction is necessary for phosphate‐retentive soils. (A. single extraction was approximately 40% effective for removing indigenous SO₄ from a Hydric Dystrandept subsoil, approximately 78% effective for an Eutrustox.) Organic materials are removed from the extracts by adsorption on charcoal; SO₄ is concentrated in the extract by volume reduction; a SO₄ spike is added; BaCl₂‐seed crystal is added, after which volume is increased by adding BaCl₂ solution. Optical density is read at 600 nm.     

Evaluation of an anion‐exchange membrane for extracting plant available phosphorus in soils

Abstract

Anion‐exchange resins (AER) have been used to determine plant available phosphorus (P) since the fifties and their results have shown strong relationships with plant growth and P uptake irrespective of soil properties. However, this procedure is still not widely used by laboratories because of difficulties in handling resin beads under routine conditions. New kinds and different shapes of resins are being produced each with specific characteristics that must be evaluated before use in laboratory procedures. Thus the objective of this work was to evaluate an AER manufactured in membranes reinforced with a Modacrylic fabric. These anion‐exchange membrane (AEM) sheets are commercially available, making them suitable for soil testing. The membranes were cut in pieces (1.0×7.5 cm) identified as AEM‐strips. The AEM‐strips were soaked in 0.5M HCl for a few days and transferred, after being rinsed with deionized water (DI), to 0.5M NaHCO₃ to convert them to HCO₃ ^‐ form. The AEM‐strips and resin beads in nylon bags recovered 98.4 and 98.0% of the P content in an aqueous P solution, respectively. Three eluent solutions were evaluated with different shaking times. The 0.1M H₂SO₄ and 1.0M NaCl in 0.1M HCl were equally suitable for the molybdenum blue color development without any pH adjustment, while the pH of the 0.5M HCl was too low. The elution of P from the AEM‐strips was independent of time with a 15‐min shaking being adequate for removal of all P from the strips. A comparison of soil sample preparation demonstrated that it was not necessary to vigorously grind or sieve the soil to improve the repeatability of the results. The AEM‐strips were compared with other methods (Pi impregnated filter paper, Mehlich I and Bray 1) using 32 soils from Guatemala with widely varying physico‐chemical and mineralogical properties. Phosphorus extracted by the AEM and Pi procedures (similar principle) were highly correlated and gave similar results irrespective of soil type. The acid extraction (Mehlich I and Bray 1 methods) attacked soil components (apatites) resulting in higher and inconsistent amounts of P extracted which may not be available to plants; the correlation between these methods within soils of similar properties was good, but when all soils were considered together the relationship was not significant. This demonstrated that the acid extraction method for P is not suitable for soils containing apatites, while those based on a sink for P (AEM and Pi) can be applied irrespective of the type of soil.