Effect of Nitrogen Fertilizers on Zinc Accumulation in Fescue

Abstract

This research evaluated effects of nitrogen fertilizers on availability of zinc (Zn) in soils. Two slit loams of the Hadley series (Typic Udifluvents) were used. Zinc sulfate was mixed with the soils to give Zn at 125, 250, 500, or 1,000 mg/kg and incubated for 14 days. Fertilizers (compost, cow manure, urea) were mixed with the soils to supply N at 200 mg/kg. Fourteen days after the fertilizers were mixed with the Zn‐treated soils, soil samples were taken for analysis of plant‐available Zn by extraction with Morgan's solution or water. After the soil samples were taken, fescue (Festuca arundinacea Schreb.) seeds were placed into pots to assess germination, growth, and Zn accumulation. Higher concentrations of Morgan's extractable Zn were detected in soils treated with compost (201 mg/kg) than with calcium nitrate (179 mg/kg), manure (153 mg/kg), or urea (152 mg/kg). However, with water extraction, higher Zn concentrations were detected in soils treated with calcium nitrate (36 mg/kg) with the lowest concentrations being extracted from soils treated with urea (8 mg/kg). Extraction of Zn by Morgan's solution or water increased as the soil‐Zn levels increased. Fescue germinated and grew at all of the soil‐Zn levels. The highest concentration of Zn occurred in plants grown in soils amended with calcium nitrate or urea, and the lowest concentration was in plants grown in soils amended with compost or manure. Fescue grown in soils amended with urea had the largest dry mass, and plants grown with compost or manure had the smallest. Zinc concentration and accumulation for fescue shoots increased as the soil‐Zn levels increased. These results suggest that accumulation of Zn in fescue can be enhanced by selection of nitrogen‐containing fertilizers that affect the solubility of Zn in soils.     

Effect of dicyandiamide on growth and nutrient uptake of cotton

Abstract

The nitrification inhibitor dicyandiamide (DCD) offers potential for improving efficiency of N applications to cotton grown on sandy soils of the southeastern Coastal Plain. Research has indicated that cotton is sensitive to DCD. The purpose of this greenhouse experiment was to investigate the effect of DCD on growth and nutrient uptake of DPL 90 cotton grown for 73 days in pots containing a typical Coastal Plain soil (Norfolk sandy loam, Typic Paleudult). Nitrogen (50 mg kg^‐1) as NaNO₃ or urea, and DCD (0, 2.5, 5, 10, 15 and 20 mg kg^‐1) were applied to the soil at first true leaf and plants were harvested 58 days later. Sodium nitrate increased leaf dry weight and total dry weight of plants 9.1 and 6.0%, respectively, over urea fertilized plants. Leaf area, dryweight, and stem dry weight were reduced linearly with DCD. Fertilization with urea increased concentrations of leaf P, K, and Mn and reduced the concentration of Mg in leaf tissue. Dicyandiamide increased leaf N, P, and K concentrations but reduced concentrations of Ca, Mg, and Mn. Uptake rates (μg^‐1 g^‐1 fresh root day^‐1) of Ca and Mg were increased 7.5 and 13.7%, respectively, with NaNO₃ vs. urea, while P uptake rate was 15.5% greater for urea‐fertilized plants vs. NaNO₃‐fertilized plants. Dicyandiamide reduced Ca and Mg uptake rates. Phosphorus uptake rates were increased by DCD when urea was the N source. The effects of DCD on cotton growth and nutrient uptake generally resulted from the compound itself and were not an indirect result of nitrification inhibition. Although significant reductions in plant growth did not occur unless DCD exceeded that normally applied with recommended N rates on this soil, these results suggest a need for caution when applying DCD to cotton grown on sandy soils.     

Correlations between Phosphorus Fractions and Total Leachate Phosphorus from Cattle Manure– and Swine Manure–Amended Soil

Although many studies have examined the effect of different application rates of cattle manure, swine manure, and urea fertilizer on the distribution of phosphorus (P) fractions in soil, few studies have correlated P fractions in soil with inorganic P (P_i) and organic P (P_o) in leachates. As part of a long-term field study, cattle and swine manures were applied to a loamy soil based on a nitrogen (N) content equivalent of 100 (low) and 400 (high) kg total N ha^?1 yr^?1 and were compared to urea fertilizer at 100 kg N ha^?1 yr^?1 and an unamended control soil. Readily available P_i [resin and sodium bicarbonate (NaHCO₃)] was significantly greater in cattle manure– and swine manure–amended soil at a high application rate than in the control. With some exceptions, urea did not significantly affect P fractions in sequentially extracted P pools. Leaching of P_i and P_o was at levels of environmental concern when cattle and swine manures were applied at the high application rate but not at the low application rate. Cattle manure had significantly greater concentrations of P_i and P_o removed by leaching compared to swine manure, most likely because of its narrow N/P ratio and greater amount of P added. Positive correlations were observed between resin P_i and total leachate P_i and between NaHCO₃-P_i and total leachate P_i, indicating the value of these measurements in predicting P mobility. The results suggest that a threshold (40 μg P g^?1 of soil) must be exceeded before a positive correlation occurs.     

EFFECT OF SOIL AMENDMENT WITH THIN STILLAGE AND GLYCEROL ON PLANT GROWTH AND SOIL PROPERTIES

Controlled environment experiments were set up in 2007 and 2008 to evaluate the potential of using by-products of the biofuel industry as soil amendments to improve fertility and plant growth in Saskatchewan soils. Trials were run with thin stillage (a by-product of ethanol production) and glycerol (by-product of biodiesel production). Canola (B. napus L.) and wheat (T. aestivum) were grown as the test crop in amended pots. Plant yield, composition, and soil properties were measured after five weeks. The stillage was found to be an effective soil amendment for increasing plant biomass yield. Per unit of nitrogen (N) added, canola yields were less than that of urea when nitrogen was the only limitation, due to only a portion of the nitrogen in the thin stillage becoming available over the five week period. However, when nutrients other than nitrogen were limiting, canola dry matter yields with thin stillage amendment approached or exceeded that of urea, due to the ability of the amendments to supply other nutrients such as phosphorus in addition to nitrogen. Glycerol, an amendment that only contains carbon, hydrogen and oxygen, was effective in increasing soil organic carbon content, but required supplemental fertilizer to account for nutrient tie-up by microorganisms during decomposition in the soil. The amendments did not have any biologically significant effects on other soil chemical parameters measured, including soluble metals, pH or salinity.     

Labile organic matter fractions as early‐season nitrogen supply indicators in manure‐amended soils

Soil test indicators are needed to predict the contribution of soil organic N to crop N requirements. Labile organic matter (OM) fractions containing C and N are readily metabolized by soil microorganisms, which leads to N mineralization and contributes to the soil N supply to crops. The objective of this study was to identify labile OM fractions that could be indicators of the soil N supply by evaluating the relationship between the soil N supply, the C and N concentrations, and C/N ratios of water extractable OM, hot‐water extractable OM, particulate OM, microbial biomass, and salt extractable OM. Labile OM fractions were measured before planting spring wheat (Triticum aestivum L.) in fertilized soils and the soil N supply was determined from the wheat N uptake and soil mineral N concentration after 6 weeks. Prior to the study, fertilized sandy loam and silty clay soils received three annual applications of 90 kg available N (ha · y)^?1 from mineral fertilizer, liquid dairy cattle manure, liquid swine manure or solid poultry litter, and there was a zero‐N control. Water extractable organic N was the only labile OM fraction to be affected by fertilization in both soil types (P < 0.01). Across both test soils, the soil N supply was significantly correlated with the particulate OM N (r = 0.87, P < 0.001), the particulate OM C (r = 0.83, P < 0.001), and hot‐water extractable organic N (r = 0.81, P < 0.001). We conclude that pre‐planting concentrations of particulate OM and hot‐water extractable organic N could be early season indicators of the soil N supply in fertilized soils of the Saint Lawrence River Lowlands in Quebec, Canada. The suitability of these pre‐planting indicators to predict the soil N supply under field conditions and in fertilized soils from other regions remains to be determined.     

用离子交换树脂膜方法预测有效氮和硫的供应及氮硫肥对油菜和小麦生长的影响

Field experiments were conducted with five rates （0, 75, 150, 225, and 450 kg P205 ha^-1） of seedbed P fertilizer application to investigate the yield of tomato in response to fertilizer P rate on calcareous soils with widely different levels of Olsen P （13-142 mg kg^-1） at 15 sites in some suburban counties of Beijing in 1999. Under the condition of no P fertilizer application, tomato yield generally increased with an increase in soil test P levels, and the agronomic level for soil testing P measured with Olsen method was 50 or 82 mg kg^-1 soil to achieve 85% or 95% of maximum tomato yield, respectively. With regard to marketable yield, in the fields where Olsen-P levels were 〈 50 mg kg^-1, noticeable responses to applied P were observed. On the basis of a linear plateau regression, the optimum seedbed P application rate in the P-insufflcient fields was 125 kg P205 ha^-1 or about 1.5-2 times the P removal from harvested tomato plants. In contrast, in fields with moderate （50 〈 Olsen P 〈 90 mg kg^-1） or high （Olsen P 〉 90 mg kg^-1） available P, there was no marked effect on tomato fruit yield. Field survey data indicated that in most fields with conventional P management, a P surplus typically occurred. Thus, once the soil test P level reached the optimum for crop yield, it was recommended that P fertilizer application be restricted or eliminated to minimize negative environmental effects.     

Effect of sulfur additions on the yield and elemental composition of canola and spring wheat 1

Growth chamber experiments of canola (Brassica napus L.) and spring wheat (Triticum aestivum L.) were conducted using three soils testing low in extractable sulfate (9–12 mg/kg). Experiments were designed to determine how the three soils compared in plant available sulfur (S) and to test the effects of various rates of potassium sulfate (K₂SO₄) and gypsum on the elemental composition and dry matter yield of the two crops. Based upon plant response, the Queens sandy loam soil appeared to have the least amount of plant available S of the three soils. There was no yield increase associated with S applied to either crop. Canola plants in each soil responded to increased applications of S with greater S uptake and considerably lower nitrogen:sulfur (N:S) ratios. Of the three soils used, only the wheat plants grown in the Queens soil responded to S applications through increased S uptake and a lower N:S ratio. Both sources of S were equally effective in providing available S to the plants.     

Using Wood Ash to Ameliorate Acidity and Improve Phosphorus Availability in Soils Amended with Partially Decomposed Cattle or Chicken Manure

The application of partially decomposed animal manure can acidify the soil by nitrification and may cause problems with phosphorus (P) availability. This study investigated the influence of applying wood ash to two soils amended with partially decomposed cattle or chicken manure on pH and P. The treatments consisted of two soils, a clay loam and sandy loam, each amended with partially decomposed chicken or cattle manure applied at 0, 5, or 15 t ha^?1, and wood ash was applied to each manure treatment at rates of 0 or 2 t ha^?1. The addition of wood ash significantly increased pH, thereby making more P available in soil and maize (Zea mays L.) tissues for both soils after being amended by manure. Both chicken and cattle manure significantly increased all the measured variables compared to the unamended soils. These results suggest that wood ash is an important amendment that could be used to amend partially decomposed manure, thereby not jeopardizing P availability to crops.     

Differential tolerance of canola to arsenic when grown hydroponically or in soil 1

Separate studies found canola tolerant to arsenic (As) when grown in hydroponic solutions and generally sensitive to As when grown in soil. Fourteen‐day‐old canola was transferred to pots containing either soil or nutrient solution and then grown for an additional 14 days in a growth chamber at different times for the two different media. Plants were grown in 0.25‐strength Hoagland's solution containing either 0, 0.27, 6.67, or 13.3μM As or in three soils with As added at rates of 0,5, and 10 mg.kg^‐1. Soil‐solution As concentrations were determined via column displacement and were the same or less (0.147 to 4.27μM) than the hydroponic As concentrations. Soil‐solution phosphorus (P) concentrations were determined in the same manner and averaged 9.28μM P compared to 500μM P from the hydroponic solutions. Chlorosis, wilting, and stunted growth—symptoms of As toxicity—occurred in canola at the highest As rate in two of the three soils used. Dry‐matter yields from the third soil were low from all treatments and a lack of response to As additions was probably due to injury from other soil‐related factors. Shoot As concentrations were generally similar from both experiments. Canola did not suffer a yield loss or exhibit As toxicity symptoms when grown in the hydroponic solutions. Leaf P was 8,000 mg.kg^‐1 in the hydroponically‐grown canola and 100 mg.kg^‐1 for the soil‐grown canola. These values are greater than (hydroponic) and lower than (soil media) sufficiency levels for plants similar to canola. High solution P concentrations in the hydroponic solution may have detoxified As by competing with As for uptake in the solution and during metabolism. Phosphate should be added to hydroponic solutions in As studies at levels close to normal soil P levels and added daily to replenish plant uptake.     

Utilizing Composted Beef Cattle Manure and Slaughterhouse Waste as Nitrogen and Phosphorus Fertilizers for Calcareous Soil

Effectively utilizing composts requires that their nitrogen (N) and phosphorus (P) contents be used as fertilizer, but how this is best accomplished is not fully understood. The authors' objective was to quantify N and P availability of a calcareous clay loam soil receiving composts derived from four contrasting beef cattle feedlot feedstocks applied at 50, 150, and 450 mg total P kg^?1 and supplemented twice with fertilizer N for a 42-week greenhouse plant bioassay. Three composted manures from beef cattle fed distinct diets and a composted mix of slaughterhouse and construction waste were applied. Inorganically fertilized and non-amended soils were included as controls. Canola (Brassica napus L.) and pea (Pisum sativum L.) were grown in pots containing 1.5 kg air-dried soil for six alternating 7-week cycles. Soils amended with composted manure from beef cattle fed typical finishing diets had the lowest apparent N recovery (31%) and the greatest soil nitrate after 42 weeks (25 mg N kg^?1). Phosphorus availability was greater with composted manure from beef cattle fed distillers' dried grains than composted manure from beef cattle fed typical finishing diets and a composted mixture of slaughterhouse and construction waste. Apparent P recovery (66%) was greatest from composted manure of beef cattle fed corn (Zea mays L.) distillers' dried grains applied at 50 mg total P kg^?1. Composted manure from beef cattle fed distillers' dried grains had greater P availability than conventional composted beef cattle feedlot manure. Overall, performance of the composted mixture of slaughterhouse and construction waste was similar to the composted beef cattle manures.     

Assessment of urea coated with pomegranate fruit powder as N slow-release fertilizer in maize

It has been hypothesized that plant polyphenol plus lignin, cellulose, and hemicelluloses can immobilize/remineralize inorganic fertilizers, such as urea, better than polyphenol alone. To test this hypothesis, urea was coated with pomegranate (Punica granatum L.) fruit powder (containing polyphenol+lignin+cellulose) at rates of 0%, 30%, 70%, and 100% (w/w) of fertilizer urea. Fertilizer nitrogen was applied at 100 and 200 mg kg^?1 soil in a clay loam soil. This natural coating material, particularly at the rate of 100% (w/w), improved the distribution of mineral nitrogen (N) and available phosphorus (P) in soil during both early and advanced growth stages of maize and significantly increased total crop N and P uptake at both rates of fertilizer nitrogen compared to urea alone. The results suggest that urea coated with the powder of pomegranate fruit could potentially be an N slow-release fertilizer for use in better synchronizing crop N demand with soil N supply.     

Effects of urea nitrogen and residual composted municipal waste and sheep manure on the growth and chemical composition of two triticale genotypes

In 2012, a greenhouse experiment was conducted to investigate the effects of field soil (C0), residual composted municipal waste (CMW), and residual composted sheep manure (SM) on the growth of triticale in pots previously growing oilseed rape in 2011. To each soil group, one of three levels of urea nitrogen (N) fertilizer was added. Results demonstrated that triticale grown in pots previously containing oilseed rape plants containing SM or CMW with 150 kg urea N ha^?1 had the highest N content. Plants grown in SM with 150 kg N ha^?1 had the greatest seed yield, but yield was not significantly different from plants grown in CMW receiving 150 kg N ha^?1. Triticale plants enriched by either SM or CMW had a higher amount of N, copper, zinc, and manganese compared to the field soil control.     

Responses of soil microbial communities to manure and biochar in wheat cultivation of a rice-wheat rotation agroecosystem in East China

Soil contamination in agroecosystems remains a global environmental problem. Biochar has been suggested as an organic amendment to alleviate soil pollution, sequester carbon(C), and improve soil fertility. However, information on how bacterial and fungal communities in acidic bulk and rhizosphere soils respond to swine manure and its biochar is still lacking. In this study, biochar and swine manure were applied at two rates of 1.5 and 3 t ha^-1 in a rice-wheat rotation field to assess ...     

Nitrification and acidification from urea application in red soil (Ferralic Cambisol) after different long-term fertilization treatments

Purpose

Long-term manure applications can prevent or reverse soil acidification by chemical nitrogen (N) fertilizer. However, the resistance to re-acidification from further chemical fertilization is unknown. The aim of this study was to examine the effect of urea application on nitrification and acidification processes in an acid red soil (Ferralic Cambisol) after long-term different field fertilization treatments.

Materials and methods

Soils were collected from six treatments of a 19-year field trial: (1) non-fertilization control, (2) chemical phosphorus and potassium (PK), (3) chemical N only (N), (4) chemical N, P, and K (NPK), (5) pig manure only (M), and (6) NPK plus M (NPKM; 70 % N from M). In a 35-day laboratory incubation experiment, the soils were incubated and examined for changes in pH, NH₄ ⁺, and NO₃ ^?, and their correlations from urea application at 80 mg N kg^?1(?80) compared to 0 rate (?0).

Results and discussion

From urea addition, manure-treated soils exhibited the highest acidification and nitrification rates due to high soil pH (5.75–6.38) and the lowest in the chemical N treated soils due to low soil pH (3.83–3.90) with no N-treated soils (pH 4.98–5.12) fell between. By day 35, soil pH decreased to 5.21 and 5.81 (0.54 and 0.57 unit decrease) in the NPKM-80 and M-80 treatments, respectively, and to 4.69 and 4.53 (0.43 and 0.45 unit decrease) in the control-80 and PK-80 treatments, respectively, with no changes in the N-80 and NPK-80 treatments. The soil pH decrease was highly correlated with nitrification potential, and the estimated net proton released. The maximum nitrification rates (K _max) of NPKM and M soils (14.7 and 21.6 mg N kg^?1 day^?1, respectively) were significantly higher than other treatments (2.86–3.48 mg N kg^?1 day^?1). The priming effect on mineralization of organic N was high in manure treated soils.

Conclusions

Field data have shown clearly that manure amendment can prevent or reverse the acidification of the red soil. When a chemical fertilizer such as urea is applied to the soil again, however, soil acidification will occur at possibly high rates. Thus, the strategy in soil N management is continuous incorporation of manure to prevent acidification to maintain soil productivity. Further studies under field conditions are needed to provide more accurate assessments on acidification rate from chemical N fertilizer applications.     

中国青海湖地区草地蝗虫与土壤的关系

A growth chamber study was conducted to determine the relationships between the supply of soil available nitrogen （N） and sulfur （S） and canola （Brassica napus） and wheat （Triticum aestivum L. ‘Biggar＇） N and S uptake and yield in three Western Canadian soils. The suitability of one-hour burial with an anion exchange membrane （AEM） was assessed for its utility as a quick test of the available N：S balance in the soil. Canola and wheat were grown on a Luvisolic soil low in available S and on Brown and Black Chernozemic soils low in both available N and S, with different rates and combinations of N and S fertilizers applied. AEM burial was used to assess soil available nitrate and sulfate supply rates after fertilization. Dry matter yield and N and S concentrations in plant tissues were determined after 6 weeks of growth. The soil available N：S ratio determined by AEM burial closely reflected the relative supplies of available N and S as revealed in the N：S ratios of plant tissue dry matter. The highest yields were achieved where the available N：S ratio in soil and plant tissue ranged from 5 to 13. Thus, a one-hour burial of an AEM probe in the field may be a useful tool to quickly test if a balanced N and S supply is present in the soil for optimum crop yield.     

Effects of Slow‐Release Fertilizers on Tomato Growth and Nitrogen Leaching

Tomatoes (Lycopersicon esculentum Mill.) were grown in 9.46‐L plastic pots in a glasshouse for evaluation of their growth and nitrogen (N) losses through leaching. Plants were fertilized with either ammonium nitrate (AN) or one of three slow‐release N fertilizers. The slow‐release N fertilizers were Georgia Pacific liquid 30‐0‐0 (L30), Georgia Pacific granular 42‐0‐0 (N42), and Georgia Pacific granular 24‐0‐0 (N24). Each fertilizer was applied at 112 low N rate (L) and 224 high N rate (H) kg N ha^?1. The pots were filled with either a sandy soil from Florida or a loam soil from Georgia. Increasing the N rate did not influence shoot biomass at 19 days after transplanting (DAT) and increased biomass production at 77 DAT. Shoot biomass differed significantly among fertilizer treatments. The accumulation of N in shoots was significantly influenced by fertilizer source, rate, and soil type. The plants grown in the loam soil accumulated significantly more N than those grown in the sandy soil with the same treatment. In the loam soil, the highest and lowest N accumulations occurred in the N42‐H and N24‐L treatments, respectively; and in the sandy soil the corresponding treatments were AN‐H and N24‐L. The amount of N leached varied with the different fertilizers, soils, and time. The net leaching of N ranged from ?0.4% to 6.3% of the fertilizer N applied for the loam soil and 6.5% to 32.9% for the sand soil. The net amount of N leached from the loam soil at both high and low application rates declined in the following order: AN > N24 > N42 > L30; the corresponding order for the sandy soil was AN‐H > N42‐H > L30‐H > N24‐H. L30 had the least leaching potential, and ammonium nitrate had the most. Slow‐release fertilizers had significantly less leaching N than did ammonia nitrate.     

Uptake of 15N by wetland rice in response to application of 15N-labelled Sesbania rostrata and urea

 Pot experiments were carried out to evaluate the response of rice to Sesbania rostrata green manure N as compared to urea fertilizer N under flooded conditions. After growing S. rostrata for 21 days with a ¹⁵N-labelled N source, the labelled Sesbania was applied to wetland rice as a green manure and the uptake of ¹⁵N from this substrate was compared to that from labelled urea. Rice was cultivated twice in the same pots. The rice was grown for a period of 49 days in each case, separated by a period of 21 days when the soil was allowed to dry. The ¹⁵N content of the soil and shoots and roots of rice was determined and ¹⁵N balances established. The total N content of the shoots and roots of rice was determined by a non-tracer method. The percentage recovery of ¹⁵N from shoot material which was derived from urea N was more than twice that from S. rostrata. The recovery of ¹⁵N from the pots receiving both green manure and urea was low, and not significantly different from that recovered from the green manure treatment. As much as 64.5–73.5% and 40.1–41% of the ¹⁵N remained in the soil which had received green manure or urea, respectively. The overall recoveries of ¹⁵N varied between 86.5% and 94.4%. At the second harvest, the oven-dry weight of shoots was significantly (P<0.05) higher in green-manure treated pots, but the total N content did not differ significantly. Labelled N remaining in the soil after amendment with the green manure was much more available to the rice crop than that remaining after the addition of urea-N. The total recovery of labelled N (shoots plus roots) amounted to 65.5% and 74%, respectively of the residual labelled N in the two S. rostrata treatments (i.e. 19.55 mg ¹⁵N pot^–1 and 39.10 mg ¹⁵N pot^–1) and 23.2% and 23.2% of the residual labelled N in the two urea treatments (i.e. 19.55 mg ¹⁵N pot and 39.10 mg ¹⁵N pot^–1), respectively. Received: 8 December 1997     

Nitrogen Fertilization of Soybean Affects Root Growth and Nodulation on Two Soil Types in Mississippi

Greenhouse studies were conducted to evaluate the influence of nitrogen (N) sources [urea + ?N-(n-butyl) thiophosphoric triamide, NBPT (urease inhibitor) and polymer-coated urea (PCU)] and rates on soybean root characteristics, nodule formation, and biomass production on two soil types (silt loam and clay) commonly cropped to soybean in Mississippi. About 15% less belowground biomass was produced in clay soil than in silt loam soil directly corresponding to all other root parameters including root length, root area, root diameter, and nodule number. Pooled across N rates, N additions resulted in 19% and 52% decrease in belowground biomass and number of nodules, respectively, across soils compared to soybean receiving no N. The N rate was the most critical factor as it influenced all root growth parameters. Number of nodules were 24% greater with PCU than urea + NBPT. Nitrogen additions and clay soil negatively impacted soybean root growth, nodulation, and belowground biomass production.

Abbreviations: Polymer-coated urea, PCU; N-(n-butyl) thiophosphoric triamide, NBPT     

矿山生态型水蓼对施用猪粪土壤中磷的吸取净化效果

【目的】 针对猪粪不合理利用导致土壤中磷过剩和磷流失的突出问题,明确磷富集植物矿山生态型水蓼 (Polygonum hydropiper) 对施用猪粪土壤磷的吸取净化效果,为高效提取施用猪粪土壤中的过量磷,降低磷素的流失风险提供科学依据。 【方法】 采用微区模拟试验,根据农田磷肥安全用量 (< 200 kg/hm2),设1、2、3 kg/m2共3个猪粪用量,以不施猪粪为对照 (CK),共4个处理,每处理3次重复。在矿山生态型水蓼收获期 (移栽后3个月) 采集植物地上部,采用微波消解仪 (CEM MARS5, USA) 消解—全自动间断化学分析仪 (AQ2, UK) 测定植株磷含量,分析矿山生态型水蓼对猪粪处理土壤中磷的富集能力。在矿山生态型水蓼移栽前和收获后按5点采样法分别采取0—20 cm和20—40 cm土层土壤样品,测定土壤样品中的水溶性磷和有效磷含量,分析种植矿山生态型水蓼前后猪粪处理土壤中易溶性磷含量的变化情况。 【结果】 不同用量猪粪处理下,矿山生态型水蓼地上部生物量较不施猪粪处理分别显著增加了18.4、24.6和42.0 g/株,最大时高达不施猪粪处理的2.16倍。各猪粪处理矿山生态型水蓼地上部磷含量显著高于不施猪粪处理,较不施猪粪处理分别增高了0.60、0.91和1.49 g/kg,最高为施猪粪处理的1.53倍。矿山生态型水蓼地上部磷积累量随猪粪用量的增加而显著增大,较不施猪粪处理分别增加了P 84.0、124和236 mg/株,最高为不施猪粪处理的3.32倍。各处理下,种植矿山生态型水蓼后0—20 cm土层中的水溶性磷和有效磷含量与种植前相比均显著降低,水溶性磷含量降低了74.9%～81.5%,有效磷含量降低了48.9%～60.0%;20—40 cm土层的水溶性磷和有效磷含量均无明显变化。 【结论】 种植矿山生态型水蓼能显著减少表层土壤中水溶性磷和有效磷量,高效吸取净化猪粪带入土壤中磷。因此,在高磷含量的土壤上种植、收获矿山生态型水蓼,是降低土壤磷素流失风险的有效生物手段。      

Evaluation of the Nitrification Inhibitor 3,4-Dimethylpyrazole Phosphate in Two Chilean Soils

ABSTRACT

Two Chilean soils were used to evaluate the performance of the nitrification inhibitor 3,4-dimetilpirazol phosphate (DMPP) added to ammonium-sulfate-nitrate (ASN) in comparison with traditional nitrogen (N) sources and different N-application forms. Two experiments were conducted: In the first, broccoli (Brassica oleracea var. italica) plants were cultivated in pots under greenhouse conditions, and received a N-fertilization equivalent to 150 Kg N ha^?1 as ASN+DMPP (one application), urea (two splits), and sodium-potassium nitrate (three splits). In the second, ryegrass (Lolium spp.) plants were grown in pots under shading conditions. In this case, ASN+DMPP and urea were applied at N rates equivalent to 150 and 300 Kg N ha^?1 in a single application. In the first experiment, ASN+DMPP increased dry-matter production, maintained a higher N content in the soil (at least until the middle of the growing period), and improved fertilizer N-use efficiency (FUE) in one soil. There were no significant differences in N-leaching losses. In the ryegrass experiment, ASN+DMPP increased dry-matter production and FUE, while N-leaching losses were reduced. Treatments with ASN+DMPP maintained higher N levels in soil throughout the growing period, and there were no significant differences in the available N fraction between the two N rates. The use of DMPP-containing fertilizers may be a good alternative for increasing FUE.