华北地区采用无机氮测试和植株速测进行夏玉米氮肥推荐

A field experiment with a split-plot design was carried out at Dongbeiwang Farm in Beijing Municipality to establish reliable N fertilizer recommendation indices for summer maize (Zea mays L.) in northern China using the soil Nmin(mineral N) test as well as the plant nitrate and SPAD (portable chlorophyll meter readings) tests. The results showed that Nrnin sollwert (NS) 60 kg N ha^-1 at the third leaf stage and N rate of 40 to 120 kg N ha^-1 at the tenth leaf stage could meet the N requirement of summer maize with a target yield of 5.5-6 t ha^-1. Sap nitrate concentrations and SPAD chlorophyll meter readings in the latest expanded maize leaves at the tenth leaf stage were positively correlated with NS levels, indicating that plant nitrate and SPAD tests reflected the N nutritional status of maize well. Considering that winter wheat subsequently utilized N after the summer maize harvest, the 0-90 cm soil Nmin (74 kg N ha^-1) and apparent N loss (12 kg N ha^-1) in the NS60＋40 treatment were controlled at environmentally acceptable levels. Therefore NS60＋40, giving a total N supply of 100 kg N ha^-1, was considered the optimal N fertilizer input for summer maize under these experimental conditions.     

TRACING NITROGEN IN SOIL-ROOT-PETIOLE-LEAF CONTINUUM IN SUGAR BEETS: CAN SPAD-502 HELP?

This work aimed to study whether soil plant analysis development (SPAD) and its modifications (SPAD/SLA, SPAD/N) were sensitive indicators of intra-seasonal nitrogen (N) changes in the soil-root-petiole-leaf continuum in sugar beets. In a three-year, field experiment, 11 occasions of measurement per year took place. Significant intra-seasonal changes of soil [total N, nitrate (NO₃)-N], root (α-amino N), petiole (NO₃-N) and leaf [SPAD,%N, SLA, SPAD/N, SPAD/SLA, specific leaf nitrogen (SLN)] traits were found. SPAD readings failed to predict N status changes in soil and sugar beets. SPAD modifications (SPAD/SLA, SPAD/N) gave significant correlations with root α-amino N (r = ?0.80, P < 0.001, n = 33) and petiole NO₃-N (r = ?0.53, P < 0.01, n = 33) which are considered as sugar beet N status indicators. SPAD readings adjusted for intra-seasonal, leaf ontogenetic changes as they captured by SLA improved the correlations with the indicators of sugar beet N status and especially with root α-amino N. Also, SPAD/SLA gave a significantly correlation with leaf %N (r = ?0.82, P < 0.001, n = 33).     

PERFORMANCE OF DUALEX IN SPRING WHEAT FOR CROP NITROGEN STATUS ASSESSMENT,YIELD PREDICTION AND ESTIMATION OF SOIL NITRATE CONTENT

Dualex and SPAD are devices developed for the purpose of testing crop nitrogen (N) status. These instruments were used in a wheat experiment in order to compare their respective performance in assessing leaf nitrogen (N) concentration, response to N topdressing application, soil nitrate (NO₃)-N levels and in predicting grain yield. The experiment included different N rates in 2005 and 2006 in the Montérégie region of Quebec, Canada. Dualex readings correlated negatively with SPAD readings, leaf N concentration, soil NO₃-N content and wheat grain yield. SPAD alone and the ratio of SPAD to Dualex measurements (Chl/Phen) were linearly related to N application rate but no effect of N application rate was found for individual Dualex parameters. However, both SPAD and Dualex readings were affected by year effects. The Dualex was also capable of indirect evaluation of in-season soil NO₃-N accumulation and the prediction of wheat yield, but more so as Chl/Phen.     

Correlation of leaf chlorophyll readings and stem nitrate concentrations in peppermint

Abstract

The SPAD chlorophyll meter appears promising for rapid, on‐farm analysis of crop nitrogen (N) status. Leaf SPAD chlorophyll levels have been correlated with total leaf N concentrations, but it has not been determined how they relate to other widely applied N diagnoses such as petiole or stem nitrate (NO₃) analysis. Our objective was to examine the relationship between leaf SPAD readings and stem NO₃ levels in peppermint (Mentha piperita L.). Upper canopy SPAD chlorophyll and stem NO₃ concentrations were determined weekly during two seasons for peppermint grown with variable N inputs. Leaf SPAD levels exhibited significant linear‐plateau responses with respect to stem NO₃, indicating that SPAD readings do not respond to luxury N consumption. The meter is therefore promising for the detection of crop N deficiencies by comparison of production fields to well fertilized plots or strips. Break‐points in the linear‐plateau regressions describe saturation concentrations of stem NO₃ with respect to leaf SPAD levels peaking at 12,000 mg NO₃‐N/kg in mid to late July and declining later in the season. The SPAD meter may be applied directly to N management by use of reference plots or it may be used as a tool to aid in determination of criteria for other diagnoses such as tissue NO₃.     

Ammonium concentration in solution affects chlorophyll meter readings in tomato leaves

Abstract

Combinations of NH₄‐N:NO₃‐N usually result in higher tomato (Lycopersicon esculentum Mill.) yields than when either form of nitrogen (N) was used alone. Leaf chlorophyll content is closely related to leaf N content, but the effect of the NH₄‐N:NO₃‐N ratio on leaf greenness was not clear. The objective of this study was to determine the influence of NH₄‐N:NO₃‐N ratios on chlorophyll meter (SPAD) readings, and evaluate the meter as a N status estimator and tomato yield predictor in greenhouse production systems. Fruit yield and SPAD readings increased as the amount of NH₄‐N in solution increased up to 25%, while higher ratios of NH₄‐N resulted in a decline in both. The N concentration in tomato leaves increased as concentration of NH₄‐N in solution increased. Fruit yield increased as chlorophyll readings increased. SPAD readings, total N in leaves, fresh weight of shoots, and fruit yield all showed a quadratic response to NH₄‐N, reaching a peak at 25 or 50% of N as NH₄‐N. SPAD readings taken at the vegetative and flowering stages of growth had the highest correlation (r²=0.54) with N concentration in leaves, but this could not be used as a reliable estimate of N status and fruit yield. Lack of correspondence between high N concentration values and fruit yield indicated a detrimental effect of NH₄‐N on chlorophyll molecules or chloroplast structure. The SPAD readings, however, may be used to determine the optimum NH₄‐N concentration in solution to maximize fruit yield.     

Leaf chlorophyll readings as an indicator of nitrogen status and yield of spinach (Spinacia oleracea L.) grown in soils amended with Luecaena leucocephala prunings

A field study was conducted to evaluate the nitrogen status and yield of spinach grown in soils amended with prunings of Leucaena leucocephala, (applied at a rate of 3, 5, 7 or 11 t ha^?1). A ‘no fertilizer’ 0 nitrogen (N) and 150 kg N ha^?1 (recommended) were the control treatments. SPAD readings were recorded for the top six leaves. Nitrogen sufficiency indices were used to indicate the N status of plants. Application of L. leucocephala prunings increased spinach yields (8.98–13.86 t DM ha^?1) relative to the 0N treatment (1.35 t DM ha^?1) and yields increased with increasing rate of pruning application. SPAD readings showed a linear increase with the increase in applied prunings. There was preferential distribution of N to upper leaves. The relationship between shoot N concentration and SPAD readings was linear and strongest for the top three leaves (r² = 0.84–0.92). The results indicate the potential of chlorophyll meter readings in assessing N status of leafy vegetables grown on soils amended with different levels of legume tree prunings.     

Determination of cotton nitrogen status with a handheld chlorophyll meter

The ability of a hand‐held chlorophyll meter (SPAD‐502 Chlorophyll Meter³, Minolta Camera Co., Ltd., Japan) to determine the N status of cotton (Gossypium hirsutum L.) was studied at field sites in Alabama and Missouri. Meter readings on the uppermost fully‐expanded leaf were compared to leaf‐blade N and petiole NO₃‐N at first square, first bloom and midbloom as to their seed cotton yield predictive capability. Nitrogen was applied at rates of 0, 45, 90, 135, 180 and 225 kg ha^‐1 to establish a range of cotton chlorophyll levels, tissue N concentrations, and seed cotton yields. A typical curvilinear cotton yield response to N fertilizer was observed in Alabama experiments.

Because of adverse weather conditions, cotton yield in Missouri experiments did not respond to N. Chlorophyll meter readings were significantly correlated to leaf‐blade N concentration at all three stages of growth for all experiments. In Alabama, chlorophyll meter readings compared favorably to leaf‐blade N and petiole NO₃‐N with respect to their seed cotton yield predictive capability at all three stages of growth. It appears that hand‐held chlorophyll meters would be as reliable as leaf‐blade N and petiole NO₃‐N for predicting supplemental N fertilization requirements of cotton. However, more research will be required prior to use of chlorophyll meter readings for routine cotton‐N recommendation purposes.     

氮肥形态及配比对菠菜生长和安全品质的影响

【目的】铵态氮肥和硝态氮肥是蔬菜生长过程中经常施用的氮肥种类,氮肥形态及配比对蔬菜生长和安全品质有着重要影响。菠菜是一种叶菜类蔬菜,富含矿质元素、维生素C和维生素E。本文通过施用铵态氮和硝态氮肥,探究氮肥形态及其配比(NH+4-N/NO-3-N)对菠菜生长和安全品质的影响。【方法】采用水培试验,设置5种不同氮素形态配比(NH+4-N/NO-3-N比值分别为100∶0、75∶25、50∶50、25∶75和0∶100)的营养液,定期采集菠菜样品并测定菠菜的生物量、株高、根系长度、硝酸盐和亚硝酸盐、有机酸和氨基酸参数值。【结果】随着NH+4-N/NO-3-N比值从100∶0变化到0∶100,菠菜的生物量、株高、根系长度、硝酸盐和亚硝酸盐累积量以及有机酸含量均呈增加趋势,而氨基酸总量则明显下降;当NH+4-N/NO-3-N比值为0∶100时,菠菜茎叶生物量为6.2g/plant,株高和根系长度分别为16.3 cm和22.5 cm,分别是NH+4-N/NO-3-N比值为100∶0时的6倍、2.2倍和2.0倍,表明菠菜是一种喜硝酸盐氮的蔬菜;当NH+4-N/NO-3-N比值由0∶100变为25∶75时,即在氮肥组合中增加25%的铵态氮肥,此时的硝酸盐和亚硝酸盐含量分别由398.5 mg/kg、1.42 mg/kg降为249.1 mg/kg、0.98mg/kg,降幅为37.5%和8.0%,表明在菠菜生长过程中适当增施铵态氮肥可有效降低硝酸盐和亚硝酸盐在茎叶中的累积;当NH+4-N/NO-3-N比值从100∶0变化到0∶100,对6种有机酸(苹果酸、富马酸、琥珀酸、α-酮戊二酸、柠檬酸和丙酮酸)而言,增加幅度最大的是富马酸,约8.6倍,增加幅度最小的是柠檬酸,约2.5倍,苹果酸则在NH+4-N/NO-3-N=25∶75时达到最大值,为985.3 mg/L;随着NH+4-N比例的减少,菠菜茎叶中的氨基酸总量呈下降趋势,NH+4-N/NO-3-N比值为100∶0、75∶25、50∶50、25∶75和0∶100的氨基酸总量分别为21.80μmol/g、12.92μmol/g、9.20μmol/g、8.30μmol/g和7.50μmol/g,表明菠菜的营养价值降低,这一趋势与上述所研究的指标(株高、根系长度、硝酸盐和亚硝酸盐含量以及有机酸含量)有着明显的区别。【结论】菠菜是一种典型的喜硝态氮类蔬菜,施用硝态氮肥可明显提高菠菜产量,但过高的施用量可导致菠菜安全品质下降。适当增施铵态氮肥可降低硝酸盐和亚硝酸盐在菠菜体内的累积,并有效调节氨基酸和有机酸的代谢。因此,在菠菜种植过程,应该合理地搭配铵态氮肥和硝态氮肥,以便在保证安全性和营养价值的基础上获取最大的生物量。     

Tomato leaf chlorophyll meter readings as affected by variety,nitrogen form,and nighttime nutrient solution strength

Leaf chlorophyll content is closely related to leaf nitrogen (N) content, so it is reasonable to assume that ammonium‐N (NH₄‐N): nitrate‐N (NO₃‐N) ratio in the nutrient solution used to grow tomatoes (Lycopersicon esculentum Mill.) hydroponically may affect leaf greenness, and consequently chlorophyll meter (SPAD) readings. It has also been shown that increasing nutrient solution strength (NSS) increases tomato productivity, but there are no reports regarding how NSS affects SPAD readings under greenhouse conditions. Genotype may also influence SPAD readings, and standardization for cultivar and sampling time may be needed. The objective of this study was to characterize SPAD readings for five tomato cultivars and SPAD reading response to a combination of two NSS (1X and 4X Steiner solution strength daily applied 18 days after transplanting at 7 p.m.) and two concentrations of NH₄‐N in solution (0 and 25%) in order to evaluate the potential of SPAD readings as a tomato yield predictor in greenhouse production systems. The SPAD readings were not uniform across tomato varieties tested, being consistently higher for ‘Max’ and lower for the other varieties. Initially, SPAD readings for tomato varieties used in this study were low at the vegetative stage, and increased up to 40 DAT, but subsequently decreased at 49 DAT, or the fruit set of the first and second clusters. After this time, SPAD readings showed no variation. Chlorophyll meter readings for ‘Max’ were higher in the top plant layers, but decreased in the top plant layer of the other tomato varieties. The SPAD readings were higher for plants supplied with 25% NH₄‐N than those without NH₄‐N in solution, but the use of a nighttime nutrient solution did not affect SPAD readings. None of the possible interactions among tomato variety, NH₄‐N: NO₃‐N ratio, and NSS were consistently significant.     

TOMATO LEAF CHLOROPHYLL METER READINGS AS AFFECTED BY VARIETY,NITROGEN FORM,AND NIGHTTIME NUTRIENT SOLUTION STRENGTH

ABSTRACT

Leaf chlorophyll content is closely related to leaf nitrogen (N) content, so it is reasonable to assume that NH₄–N:NO₃–N ratio in the nutrient solution used to grow tomatoes (Lycopersicon esculentum Mill.) hydroponically may affect leaf greenness, and consequently chlorophyll meter (SPAD) readings. It has also been shown that increasing nutrient solution strength (NSS) increases tomato productivity, but there are no reports regarding how NSS affects SPAD readings under greenhouse conditions. Genotype may also influence SPAD readings, and standardization for cultivar and sampling time may be needed. The objective of this study was to characterize SPAD readings for five tomato cultivars, and SPAD reading response to a combination of two nutrient solutions strength (NSS) (1X and 4X Steiner solution strength daily applied 18 days after transplanting at 7 p.m.) and two concentrations of NH₄–N in solution (0 and 25%) in order to evaluate the potential of SPAD readings as a tomato yield predictor in greenhouse production systems. The SPAD readings were not uniform across tomato varieties tested, being consistently higher for “Max” and lower for the other varieties. Initially, SPAD readings for tomato varieties used in this study were low at the vegetative stage, and increased up to 40 days after transplant (DAT), but subsequently decreased at 49 DAT, or the fruit set of the first and second clusters. After this time, SPAD readings showed no variation. Chlorophyll meter readings for Max were higher in the top plant layers, but decreased in the top plant layer of the other tomato varieties. The SPAD readings were higher for plants supplied with 25% NH₄–N than those without NH₄–N in solution, but the use of a nighttime nutrient solution did not affect SPAD readings. None of the possible interactions among tomato variety, NH₄–N:NO₃–N ratio, and NSS were consistently significant. SAPD readings may be useful in monitoring low or high supply of N in greenhouse grown tomato plants.     

Evaluation of the Minolta SPAD‐502 chlorophyll meter for nitrogen management in corn

The field study investigated the relationship of Minolta SPAD 502 (SPAD) readings to applied nitrogen (N) fertilizer rate, corn (Zea mays L.) yield, and leaf N concentration. The experiment was conducted on a total of six sites in Illinois during 1991 and 1992. Ten different open pedigree corn hybrids were grown at a final population of 65,000 plants ha^‐1. Nitrogen was applied at four rates(0, 90, 180, and 270 kgN ha^‐1) as 28% liquid N solution. Significant main effects of environment (E), and hybrid (H), and E x H interaction were detected for all measured parameters. SPAD readings and leaf N concentration at all sampling times (V7, R1, and R4) as well as grain N concentration were affected by N fertilizer rate. Maximum mean grain yield and maximum grain N concentration were obtained at 110 and 195 kg N ha^‐1, respectively. At all sampling times the correlation of SPAD readings to N fertilizer rate were low but significant (R=0.22 at V7 and R1, R=0.11 at R4). SPAD correlation to corresponding leaf N concentration improved over time. The Pearson correlation was R=0.33 at V7 and increased to R=0.78 at R4. The SPAD meter did a good job at providing a measure of the relative greenness of living leaves at a specific point in time. Chlorophyll readings can therefore be useful in detecting N deficiencies in growing crops. But, the SPAD meter cannot be used to make accurate predictions of how much fertilizer N will be needed by a crop during the future growing season. We conclude then that the SPAD meter will be most useful as a diagnostic aid rather than a tool for N management in corn.     

Nitrate Dynamic in a Tropical Mollisol Amended with Organic Manures,Planted with Sweet Corn,and Monitored with SPAD Readings

Two field experiments were conducted to evaluate the use of relative chlorophyll content (RCC) in sweet corn leaves as an indicator of nitrate concentration in soil. Study objectives were (1) to evaluate the effect of manure types (chicken and dairy), rates (0, 168, 336, and 672 kg ha^?1 equivalent N), and application frequency (one and two applications) of organic amendments on (a) nitrate nitrogen (NO₃-N) concentration within and below the crop root zone and (b) the RCC of sweet corn leaves and (2) to quantify the relationship between RCC and NO₃-N concentrations. The results showed a significant increase in NO₃-N concentrations within and below the root zone and plant leaf RCC under chicken manure treatments. Manure application rates and frequency had a significant effect on both NO₃-N concentration within and below the root zone and leaf RCC. It appears that leaf RCC can be used as an indicator of NO₃-N availability for sweet corn cultivated under the study conditions.     

Effect of Nitrogen Fertilizer Forms on Growth,Photosynthesis, and Yield of Rice Under Cadmium Stress

ABSTRACT

A pot experiment was conducted to study the influence of four nitrogen (N) fertilizer forms [Urea; calcium nitrate, Ca(NO₃)₂; ammonium sulfate, (NH₄)₂SO₄; and organic N] on growth, photosynthesis, and yield of rice under two cadmium (Cd) levels (0 and 100 mg Cd kg^?1 soil). Cadmium addition significantly reduced photosynthetic rate, and the reduction varied with N fertilizer form, with ammonium (NH₄ ⁺)-N and urea treated plants having more reduction. Nitrogen form had a distinct effect on SPAD value, and the effect was also dependent on Cd level and growth stage. Cadmium-stress significantly reduced flag leaf area, but for the second leaf, only the plants supplied with organic N showed the reduction. There was a significant difference in plant height among four N forms, with NH₄ ⁺- and nitrate (NO₃ ^?)-treated plants having the highest and lowest height, respectively. Cadmium stress caused significant reduction in grains per panicle and total plant weight, and the reduction varied with N form, with organic N treatment showing more reduction. There were significant differences among N forms in N and Cd concentrations of the plants subjected to Cd stress, with NH₄ ⁺-N treated plants having highest N and lowest Cd concentrations and NO₃ ^?-treated plants having lowest N and highest Cd uptake. The results showed that the inhibition of Cd stress on growth and yield formation of rice is closely related to N fertilizer form.     

Einfluß der NO3- bzw. NH4-Ernährung auf Ertrag und Nitratgehalt von Spinat und Kopfsalat

Influence of NO₃ and NH₄-nutrition on yield and nitrate content of spinach and lettuce Field and pot experiments were conducted in order to study the effect of N-supply (N_min + N-fertilization) and partial and complete NH₄-nutrition on yield and nitrate content of spinach and lettuce. The highest yields were obtained when the N-supply of spring spinach was 250 kg N/ha, autumn spinach 200 kg N/ha and lettuce 120 kg N/ha. Nitrate content of plants could be diminished by reducing N-supply. However, if N-supply is below the optimum value yield depressions are inevitable. NH₄-nutrition reduced in both species nitrate content. But, spinach yield was reduced heavily. Very low nitrate content of plants occured, when N-nutrition of plants cultivated in nutrient solution, was changed from NO₃ to NH₄ during the last days before harvest. Herewith similar weights and head quality were obtained as with permanent NO₃-nutrition.     

Corn (Zea mays L.) Grain and Stover Yield as Affected by Soil Residual Mineral Nitrogen

Evaluation of nitrogen (N) dynamic in soil using regression equations is important for proper determination of N fertilization. A 3-year field experiment was conducted to (1) develop the best-fitted regression model relating corn grain and stover yield to soil residual ammonium (NH₄)-N and nitrate (NO₃)-N for corn yield prediction and (2) evaluate how such a model can be beneficial to the health of ecosystem by predicting the appropriate rates of N fertilization for corn production. Soil NH₄-N and NO₃-N were determined at corn harvest at the depths of 0–30 and 30–60 cm. Nitrogen fertilizer rates and soil mineral N accounted for a maximum of 93% variation in corn grain yield. Soil mineral N enhanced corn yield more than N fertilizer. Totals of 63.1 and 14.1 kg/ha of soil residual NO₃-N and NH₄-N were found in the 0- to 60-cm depth, indicating the importance of performing soil N tests.     

Use of a chlorophyll meter to evaluate the nitrogen status of dryland winter wheat

Abstract

Chlorophyll meter leaf readings were compared to grain yield, leaf N concentration and soil NH₄‐N plus NO₃‐N levels from N rate studies for dryland winter wheat Soil N tests and wheat leaf N concentrations have been taken in the spring at the late tillering stage (Feekes 5) to document a crop N deficiency and to make fertilizer N recommendations. The chlorophyll meter offers another possible technique to estimate crop N status and determine the need for additional N fertilizer. Results with the chlorophyll meter indicate a positive association between chlorophyll meter readings and grain yield, leaf N concentration and soil NH₄‐N plus NO₃‐N. Additional tests are needed to evaluate other factors such as differences among locations, cultivars, soil moisture and profile N status.     

紫色土施氮对莴笋营养效应的研究

3种紫色土施用化学氮肥盆栽莴笋的研究结果表明,石灰性紫色土莴笋生长状况最差,施中、高氮明显降低其株高、根重和茎、叶产量,而中性紫色土施氮的生物效应最佳。酸性紫色土莴笋叶片((NO3--N)/(TN)100)值最低、((AAN)/(TN)100)值最高;植株氮素表观利用率为中性土酸性土石灰性紫色土。中性、酸性和石灰性紫色土施氮分别为133、67、67mg/kg土时,莴笋3个生长期叶片N/P、N/K、K/P值变异系数(C.V％)小,生物量高。施氮显著提高中性和石灰性紫色土莴笋叶片NRA(r值0.9275*～0.9956* *),降低CAT活性和Vc含量,使茎中(△NO3-)/(△FN)值高于酸性紫色土2～5倍,但却提高酸性紫色土莴笋水溶性氨基酸、粗蛋白和可溶性糖含量,改善其食用价值。     

NITRATE ACCUMULATION IN SPINACH AS INFLUENCED BY SULFUR AND PHOSPHORUS APPLICATION UNDER INCREASING NITROGEN LEVELS

Nitrate (NO₃) accumulation by spinach was studied under increasing nitrogen (N) levels (60, 120 and 240 kg N ha^?1) along with sulfur (45 kg S ha^?1) and phosphorus (P; 90 kg P₂O₅ ha^?1) application. Plants were harvested at 50 and 65 days after sowing. Plant samples were analyzed for NO₃-N and total N, P, S, potassium (K), calcium (Ca), and magnesium (Mg). Radio assay of 35S was done to estimate percent sulfur derived from fertilizer and percent fertilizer sulfur utilization. Spinach maintained a very high level of NO₃-N in its tissue throughout the growing period. NO₃-N was increased with increasing nitrogen level and was reduced with phosphorus and sulfur application and also with advancement in growth. Total N, P, S, K, Ca and Mg uptake were increased with increasing nitrogen levels as well as with application of sulfur and phosphorus. Sulfur application caused increase in percent sulfur derived from fertilizer and percent utilization of fertilizer sulfur.     

硝酸盐反射仪和SPAD法对玉米氮素营养诊断的比较

精准的营养诊断是了解作物氮素营养及推荐施肥的基础。本文在田间滴灌条件下利用SPAD叶绿素仪(SPAD-502 Plus)和硝酸盐反射仪(RQ flex10)两种诊断方法对玉米关键生育时期的氮素营养诊断进行研究,旨在筛选出适宜的诊断方法,并依据诊断值建立滴灌玉米不同生育时期的施肥模型。试验设置0 kg(N)·hm~(-2)(N0)、225 kg(N)·hm~(-2)(N225)、330 kg(N)·hm~(-2)(N330)、435 kg(N)·hm~(-2)(N435)和540 kg(N)·hm~(-2)(N540)5个施氮水平,在不同生育时期测定了玉米叶片SPAD值和叶鞘NO_3~-含量,并分别与施氮量、植株全氮含量、产量进行方程拟合,比较两种诊断方法对玉米氮素营养的响应。研究结果表明:1)玉米叶片SPAD值和叶鞘NO_3~-含量均随施氮量的增加而显著升高,且在拔节期对施氮量的响应最敏感。叶鞘NO_3~-含量对施氮量变化的响应较SPAD值大,其与施氮量及玉米产量的拟合度均高于SPAD值,说明硝酸盐反射仪法对滴灌玉米氮素丰缺的反应更灵敏。2)玉米全氮含量与叶片SPAD值呈显著线性关系,而与叶鞘NO_3~-含量则以线性加平台表示。当叶鞘NO_3~-含量小于186 mg·L~(-1)时,植株全氮与NO_3~-间呈显著线性相关;当叶鞘NO_3~-含量大于186 mg·L~(-1)时,植株全氮随NO_3~-含量增加趋于不变。3)本农作区滴灌玉米最佳经济施氮量为402.5 kg·hm~(-2),对应的玉米产量为17 049 kg·hm~(-2)。玉米拔节期、抽雄吐丝期和灌浆期的临界叶鞘NO_3~-含量分别为729.3 mg·L~(-1)、536 mg·L~(-1)和81.2 mg·L~(-1)。SPAD叶绿素仪和硝酸盐反射仪均可对滴灌玉米进行氮素营养诊断,但硝酸盐速测值能更敏感地反映氮素丰缺状况,基于硝酸盐反射法进行作物氮素营养诊断及推荐施肥具有较好的准确性与适用性。