IMPACT OF LOW RATES OF NITROGEN APPLIED AT PLANTING ON SOYBEAN NITROGEN FIXATION

Environmental conditions in the northern Great Plains can delay emergence, nitrogen (N) fixation and growth of soybean due to cool and wet soil conditions at planting. The objective was to evaluate the impact of low rates of N applied at planting on soybean N fixation and crop growth. A field experiment was established within corn soybean rotation using a split-plot design with four replications. Whole plots were no-tillage and conventional tillage and split plots were starter fertilizer. Nitrogen sources were ammonium nitrate or urea applied at four rates. The amount of plant N fixation increased with growth stage reaching a maximum fixation at the R5 growth stages. Plant ureide content decrease with increase N applied for all growth stages except R7. The increase in plant biomass contributed to an overall increase in yield indicating that in unfavorable environments application of N at planting can have a positive impact on soybean growth.     

Enhancing Corn Production Through the Use of Starter Fertilizer in the Northern Great Plains

Abstract

The northern portion of the Great Plains has environmental conditions that require unique management practices to ensure optimum corn (Zea mays L.) yield and quality. The objective was to investigate the effect of starter fertilizer on corn yield and quality under different soil management. A field experiment was established within a 2‐year corn/soybean [Glycine max (L.) Merrill] rotation. Whole‐plot treatments were tillage with split‐plot treatments of starter fertilizer. Starter fertilizer treatments consisted of two nitrogen (N) sources, each at four rates, all contained phosphorus (P) and potassium (K). An additional treatment of no starter fertilizer was also incorporated into the experiment. There was a significant increase in yield with application of starter‐N for all years except 2002. The most dramatic yield increase was obtained with the comparison between the no starter (no N, P, or K) treatment and the P and K treatment (no N+P and K). Starter fertilizer with only P and K also increased yield, oil production, and N removal in all years compared with no starter fertilizer treatment. Application of starter fertilizer can have a significant positive impact on yield and quality of corn grown in the northern Great Plains.     

Soil residual nitrogen under various crop rotations and cultural practices

Crop rotation and cultural practice may influence soil residual N available for environmental loss due to crop N uptake and N immobilization. We evaluated the effects of stacked vs . alternate‐year crop rotations and cultural practices on soil residual N (NH₄‐N and NO₃‐N contents) at the 0–125 cm depth, annualized crop N uptake, and N balance from 2005 to 2011 in the northern Great Plains, USA. Stacked rotations were durum (Triticum turgidum L.)–durum–canola (Brassica napus L.)–pea (Pisum sativum L.) (DDCP) and durum–durum–flax (Linum usitatissimum L.)–pea (DDFP). Alternate‐year rotations were durum–canola–durum–pea (DCDP) and durum–flax–durum–pea (DFDP). Both of these are legume‐based rotations because they contain legume (pea) in the crop rotation. A continuous durum (CD) was also included for comparison. Cultural practices were traditional (conventional tillage, recommended seeding rate, broadcast N fertilization, and reduced stubble height) and improved (no‐tillage, increased seeding rate, banded N fertilization, and increased stubble height) systems. The amount of N fertilizer applied to each crop in the rotation was adjusted to soil NO₃‐N content to a depth of 60 cm observed in the autumn of the previous year. Compared with other crop rotations, annualized crop biomass N was greater with DCDP and DDCP in 2007 and 2009, but was greater with DDFP than DCDP in 2011. Annualized grain N was greater with DCDP than CD, DFDP, and DDFP and greater in the improved than the traditional practice in 2010 and 2011. Soil NH₄‐N content was greater with CD than other crop rotations in the traditional practice at 0–5 cm, but was greater with DDCP than CD and DDFP in the improved practice at 50–88 cm. Soil NO₃‐N content was greater with CD than other crop rotations at 5–10 cm, but was greater with CD and DFDP than DCDP and DDCP at 10–20, 88–125, and 0–125 cm. Nitrate‐N content at 88–125 and 0–125 cm was also greater in the traditional than the improved practice. Nitrogen balance based on the difference between N inputs and outputs was greater with crop rotations than CD. Increased N fertilization rate increased soil residual N with CD, but legume N fixation increased N balance with crop rotations. Legume‐based crop rotations (all rotations except CD) reduced N input and soil residual N available for environmental loss, especially in the improved practice, by increasing crop N uptake and N immobilization compared with non‐legume monocrop.     

Soil pH and Exchangeable Cation Responses to Tillage and Fertilizer in Dryland Cropping Systems

Long-term use of nitrogen (N) fertilizers can lead to fertility-lowering soil chemical changes. To examine this in geologically young soils in the northern Great Plains of North America, we present near-surface (0–7.6 cm) soil chemistry data from 16 years of two crop rotations: continuous crop (CC; spring wheat [Triticum aestivum L.]—winter wheat [T. aestivum]—sunflower [Helianthus annuus L.]) and crop-fallow (C-F; spring wheat—fallow) that underwent factorial tillage (none, minimum, conventional) and N rate (low, medium, high) treatments. For CC, the N rate (but not tillage) had a significant effect on pH, with the high N rate leading to the largest pH decline (?0.76). The nitrogen rate also had a significant effect on cation exchange capacity (CEC) for CC, whereby CEC increased with the N rate. Managers utilizing high N rates should be aware of the potential for soil acidification, even in the northern Great Plains of North America.     

Utilization of Existing Technology to Evaluate Spring Wheat Growth and Nitrogen Nutrition in South Dakota

Abstract

Sensor‐based technologies for in‐season application of nitrogen (N) to winter wheat (Triticum aestivum L.) have been developed and are in use in the southern Great Plains. Questions arise about the suitability of this technology for spring wheat production in the northern Great Plains. A field experiment was established in Brookings, SD, to evaluate the GreenSeeker Hand Held optical sensor (NTech Industries, Ukiah, CA) for predicting in‐season N status on three spring wheat cultivars (Ingot, Oxen, and Walworth) across five N treatments. Nitrogen rates were 0, 34, 68, 102, and 136 kg N ha^?1 applied preplant as ammonium nitrate. Sensor readings and plant biomass samples were collected at Feekes 6 and Feekes 10 growth stages. The sensor measures reflectance in the red and near infrared (NIR) regions of the electromagnetic spectrum. A normalized difference vegetation index (NDVI) was calculated. The ability of the sensor readings to predict biomass, plant N concentration, and plant N uptake for each sampling date was determined. In general, biomass, plant N concentration, and N uptake increased with increasing N rate for both sampling dates. Readings collected at Feekes 6 and Feekes 10 showed a significant relationship with plant biomass, N concentration, and N uptake for all varieties. Plant N uptake and NDVI resulted in a higher regression coefficients compared to biomass and plant N concentration for all varieties. Results suggest that existing sensor‐based variable nitrogen technology developed for winter wheat could be utilized in the northern Great Plains for estimating in‐season N need for spring wheat.     

Mineral nitrogen impairs the biological nitrogen fixation in soybean of determinate and indeterminate growth types

Soybean (Glycine max Merrill) crop production in Brazil relies mainly on biological nitrogen fixation (BNF) for nitrogen (N) supply. Recent adoption of indeterminate growth-type genotypes has raised doubts on the need for supplemental mineral N that might negatively affect the BNF. We assessed the effects of mineral N on BNF attributes of soybean genotypes grown in central and southern Brazil. Genotypes were inoculated with Bradyrhizobium sp. and/or received mineral N in three sets of experiments. In the first set, two genotypes received increasing rates of mineral N in nutrient solution, which consistently reduced the BNF. In the second set, mineral N applied at sowing and/or topdressing reduced nodulation and ureides-N in determinate and indeterminate growth-type genotypes. In the third set, mineral N applied at R5.3 stage, foliar or as topdressing, did not increase grain yield in four field experiments. Mineral N impaired BNF irrespective of the growth type and had no effect on grain yield.     

Effects of co-inoculation of Bradyrhizobium elkanii BLY3-8 and Streptomyces griseoflavus P4 on Rj4 soybean varieties

Co-inoculation of selected nitrogen-fixing bacteria with plant growth-promoting bacteria is the promising way for the improvement of soybean production through enhancing plant growth, nodulation, and N₂ fixation. Therefore, this experiment was conducted to study the effects of co-inoculation of Bradyrhizobium elkanii BLY3-8 with Streptomyces griseoflavus P4 on plant growth, nodulation, N₂ fixation, N uptake, and seed yield of Rj₄ soybean varieties. Two experiments with completely randomized design and three replicates were done in this study. N₂-fixation ability of soybean was evaluated by acetylene reduction activity (ARA) and relative ureide method. In the first experiment, synergetic effect in N₂ fixation and nodulation was occurred in co-inoculation treatment (BLY3-8 + P4) in Yezin-3 and Fukuyutaka. Based on these results, co-inoculation effect of BLY3-8 and P4 was assessed on Yezin-3 and Fukuyutaka varieties at three different growth stages, using Futsukaichi soil under natural environmental conditions. This study shows that co-inoculation of BLY3-8 and P4 significantly increased N₂ fixation at V6 stage; plant growth, nodulation, N₂ fixation, and N uptake at R3.5 stage; and shoot growth, N uptake, and seed yield at R8 stage, in Rj₄ soybean varieties compared with the control. Significant difference in plant growth, nodulation, N₂ fixation, N uptake, and yield between co-inoculation and control, not between single inoculation and control, suggests that there is a synergetic effect due to co-inoculation of BLY3-8 and P4.     

Improving dryland cropping system nitrogen balance with no‐tillage and nitrogen fertilization

Studies on N balance due to N inputs and outputs and soil N retention to measure cropping system performance and environmental sustainability are limited due to the complexity of measurements of some parameters. We measured N balance based on N inputs and outputs and soil N retention under dryland agroecosystem affected by cropping system and N fertilization from 2006 to 2011 in the northern Great Plains, USA. Cropping systems were conventional tillage barley (Hordeum vulgaris L.)–fallow (CTB‐F), no‐tillage barley–fallow (NTB‐F), no‐tillage barley–pea (Pisum sativum L.) (NTB‐P), and no‐tillage continuous barley (NTCB). In these cropping systems, N was applied to barley at four rates (0, 40, 80, and 120 kg N ha^?1), but not to pea and fallow. Total N input due to N fertilization, pea N fixation, soil N mineralization, atmospheric N deposition, nonsymbiotic N fixation, and crop seed N and total N output due to grain N removal, denitrification, volatilization, N leaching, gaseous N (NO_x) emissions, surface runoff, and plant senescence were 28–37% greater with NTB‐P and NTCB than CTB‐F and NTB‐F. Total N input and output also increased with increased N rate. Nitrogen accumulation rate at the 0–120 cm soil depth ranged from –32 kg N ha^?1 y^?1 for CTB‐F to 40 kg N ha^?1 y^?1 for NTB‐P and from –22 kg N ha^?1 y^?1 for N rates of 0 kg N ha^?1 to 45 kg N ha^?1 y^?1 for 120 kg N ha^?1. Nitrogen balance ranged from 1 kg N ha^?1 y^?1 for NTB‐P to 74 kg N ha^?1 y^?1 for CTB‐F. Because of increased grain N removal but reduced N loss to the environment and N fertilizer requirement as well as efficient N cycling, NTB‐P with 40 kg N ha^?1 may enhance agronomic performance and environmental sustainability while reducing N inputs compared to other management practices.     

Starter fertilizer effects on grain sorghum hybrids grown on a soil high in residual phosphorus in a no‐tillage environment

Conservation tillage crop production systems have become common in the central Great Plains because they reduce soil erosion and increase water‐use efficiency. The high residue levels associated with no‐tillage systems can cause soils to be cool and wet which can reduce nutrient uptake and growth of crops. Starter fertilizer applications have been effective in improving nutrient uptake even on soils high in available nutrient elements. Resent research indicates that corn (Zea mays L.) hybrids differ in their responses to starter fertilizer. No information is currently available concerning grain sorghum [Sorghum bicolor (L.) Moench] hybrid response to starter fertilizer. The objective of this study was to evaluate grain sorghum hybrid responses to starter fertilizer in a no‐tillage environment on a soil high in available phosphorus (P). This field experiment was conducted from 1995 to 1997 at the North Central Kansas Experiment Field, located near Belleville, on a Crete silt loam soil (fine, montmorillonitic, mesic, Pachic Arguistoll). Treatments consisted of 12 grain sorghum hybrids and two starter fertilizer treatments. Fertilizer treatments were starter fertilizer [34 kg nitrogen (N) and 34 kg P₂O₅ ha^‐1] or no starter fertilizer. Starter fertilizer was applied 5 cm to the side and 5 cm below the seed at planting. Immediately after planting, N was balanced on all plots to give a total of 168 kg N ha^‐1. In all three years of the experiment, grain yield, total P uptake (grain plus stover), grain moisture content at harvest, and days to mid‐bloom were affected by a hybrid x starter fertilizer interaction. Starter fertilizer consistently increased yields, reduced harvest grain moisture, improved total P uptake, and reduced the number of days needed from emergence to mid‐bloom of Pioneer 8505, Pioneer 8522Y, Pioneer 8310, Dekalb 40Y, Dekalb 48, Dekalb 51, Dekalb 55, and Northrup King 524, buthadno effect on Pioneer 8699, Dekalb 39Y, Northrup King 383Y, and Northrup King 735. When averaged over the three years, starter fertilizer increased grain yield of responding hybrids (hybrids in which the 3‐year average grain yield was significantly increased by the application of starter fertilizer) by 920 kg ha^‐1. In responding hybrids, starter fertilizer reduced grain moisture at harvest by 54 g kg¹ and also shortened the period from emergence to mid‐bloom by five days. Starter fertilizer increased V6 stage aboveground dry matter production and N and P uptake of all hybrids tested. Results of this work show that in high residue production systems even on soils high in available P, starter fertilizer can consistently increase yield of some hybrids, whereas other hybrids are not affected.     

Cover crop potential of winter oilseed crops in the Northern U.S. Corn Belt

ABSTRACT

Winter camelina [WC, Camelina sativa (L.) Crantz] and field pennycress (FP, Thlaspi arvense L.) are emerging oilseed crops in corn–soybean rotations, but little is known about their cover crop potential. A 2-year study was conducted in Minnesota, USA to evaluate the effect of winter oilseed crops on nitrogen (N) use, growth and yield of corn and soybean. Treatments included WC, FP, winter rye (WR, Secale cereale L.), and a no cover crop (NC) control. Oilseed crops produced 40–50% less spring biomass and accumulated less N compared to WR. The tissue-N of WC and FP was 39.0% and 6.6% higher than WR, respectively. The C:N ratio of cover crops was lower than 20:1, suggesting rapid decomposition. Compared with NC, cover crops lowered soil nitrate before major crops planting, but the post-harvest N profile following corn and soybean was not affected. Compared with NC, cover crops significantly decreased corn yield, with 8.7%, 9.5% and 9.8% reduction following WC, FP and WR, respectively. Cover crops did not affect growth, yield and N uptake of soybean. Oilseed crops showed potential to improve N cycling in the rotation, but more research of their impact on major crops is needed.     

Nitrogen Fixation,Ureide, and Nitrate Accumulation Responses to Soybean Aphid Injury in Glycine max

There is little information available about soybean aphid (Aphis glycines Matsumura) effects on the physiology and mineral nutrition of soybean (Glycine max [L.] merr.). Controlled-environment studies were conducted to measure soybean aphid infestation effects on dry weight, nitrogen (N) fixation, ureide-N, and nitrate-N concentration and accumulation. Plants grown in perlite using –N nutrient solution culture were infested at the 3rd trifoliolate (V3) stage and measured for N fixation, nodule characteristics, and ureide-N concentration at the full pod (R4) stage. When compared to uninfested control plants, aphid infestation reduced total nodule volume per plant by 34%, nodule leghemoglobin per plant by 31%, plant N fixation rate by 80% and shoot ureide-N concentration by 20%. Soil-grown plants were infested at the first trifoliolate (V1) stage and shoots were measured for dry weight, nitrate-N, and ureide-N at the full bloom (R2) stage. Infestation reduced shoot dry weight by 63%, increased nitrate-N concentration by 75%, but did not significantly affect ureide-N concentration. Because nutrient concentration is a single-point measurement that results from the integration of two dynamic processes, nutrient accumulation and dry matter production, we conclude that aphid-induced reductions in N fixation, coupled with decreased dry weight accumulation, caused shoot ureide-N concentration to remain unchanged in aphid-injured plants when compared to uninfested plants. Because nitrate-N concentration was greater in aphid-damaged shoot tissue, we further conclude that nitrate-N accumulation was less sensitive to aphid injury than dry weight accumulation.     

Cultivar effects on nitrogen fixation in peas and lentils

Increasing nitrogen fixation in legume crops could increase cropping productivity and reduce nitrogen fertilizer use. Studies have found that crop genotype, rhizobial strain, and occasionally genotype-specific interactions affect N fixation, but this knowledge has not yet been used to evaluate or breed for greater N fixation in US crops. In this study five USDA varieties of lentils (Lens culinaris Medik.) and five varieties of peas (Pisum sativum L.) were tested with 13 to 15 commercially available strains of Rhizobium leguminoserum bv. viciae to identify the better N fixing rhizobial strains, crop varieties, and specific pairings. Peas and lentils inoculated with individual strains were grown in growth chambers for 6 week. Plants received (¹⁵NH₄)₂ SO₄ (5 at.%) starter fertilizer to measure N fixation by isotope dilution. Below- and above-ground biomass, numbers of nodules, and the proportion of plant N supplied by fixation (PNF) were determined. The percent of N fixed was significantly affected by crop variety and significantly correlated with number of nodules in both lentils and peas. This implies that one strategy for enhancing crop N fixation is developing varieties that have higher rhizobium infection rates. Total N fixation in lentils was significantly influenced by both crop variety and rhizobial strain. Eston variety lentil and Shawnee variety pea had the highest PNF of 80.8% and 91.3%, respectively. The different strains of R. leguminoserum affected PNF in lentils but not in peas. These findings suggest that N fixation improvement in lentils and peas may be addressed most effectively by breeding crops for greater N fixation hosting capacity.     

NO3‐N Soil Test—approaches to use and interpretation

Abstract

Nitrate‐nitrogen (NO₃‐N) soil tests have been instituted by several Great Plains States to aid in making nitrogen fertilizer recommendations. The NO₃‐N soil test is useful in the Great Plains because climatic conditions are such that NO₃‐N leaching is low and soil rooting depths are great.

Essentially four basic approaches to the use and interpretation of the NO₃‐N soil test have been developed and are discussed. They are: (1) total amount of NO₃‐N extracted from a two foot soil profile is available to the growing crop; (2) only a portion of the extractable NO₃‐N is available to the growing crop; (3) other mineral nitrogen, such as NH₄‐N, is just as available to the growing crop and (4) some nitrogen becomes available from the soil organic matter. The NO₃‐N soil test requires that deep samples (two feet or more) be taken and that they are air‐dried as rapidly as possible. Late fall or early spring soil samples are necessary for interpretation of the NO₃‐N soil test.     

Effects of co-inoculation of Bradyrhizobium japonicum SAY3-7 and Streptomyces griseoflavus P4 on plant growth,nodulation, nitrogen fixation,nutrient uptake,and yield of soybean in a field condition

ABSTRACT

Co-inoculation of nitrogen-fixing bacteria with plant growth-promoting bacteria has become more popular than single inoculation of rhizobia or plant-growth-promoting bacteria because of the synergy of these bacteria in increasing soybean yield and nitrogen fixation. This study was conducted to investigate the effects of Bradyrhizobium japonicum SAY3-7 and Streptomyces griseoflavus P4 co-inoculation on plant growth, nodulation, nitrogen fixation, nutrient uptake, and seed yield of the ‘Yezin-6’ soybean cultivar. Nitrogen fixation was measured using the acetylene reduction assay and ureide methods. Uptake of major nutrients [nitrogen (N), phosphorus (P), potassium (K), calcium (Ca), and magnesium (Mg)] was also measured. This study showed that single inoculation of SAY3-7 significantly increased shoot biomass; nodulation; Relative Ureide Index (RUI %), percent nitrogen derived from N fixation (% Ndfa); N, P, K, Ca, and Mg uptakes; during the later growth stages (R3.5 and R5.5), compared with control. These observations indicate that SAY3-7 is an effective N-fixing bacterium for the plant growth, nodulation, and nitrogen fixation with an ability to compete with native bradyrhizobia. Co-inoculation of SAY3-7 and P4 significantly improved nodule number; nodule dry weight; shoot and root biomass; N fixation; N, P, K, Ca, and Mg uptake; at various growth stages and seed yield in ‘Yezin-6’ soybean cultivar compared with the control, but not the single inoculation treatments. Significant differences in plant growth, nodulation, N fixation, nutrient uptake, and yield between co-inoculation and control, not between single inoculation and control, suggest that there is a synergetic effect due to co-inoculation of SAY3-7 and P4. Therefore, we conclude that Myanmar Bradyrhizobium strain SAY3-7 and P4 will be useful as effective inoculants in biofertilizer production in the future.     

Selection of type A and type B strains for improving symbiotic effectiveness on non-Rj and Rj4 genotype soybean varieties

The selection of effective rhizobia for higher efficiency nitrogen fixation is one of the most important steps for inoculant production. Therefore, this experiment was conducted to select the most effective type A and type B strains for specific Rj-gene harboring soybean varieties and to test the symbiotic effectiveness of selected strains on different Rj-gene harboring soybean varieties. Screening experiments using the specific soybean varieties were done with a completely randomized design and three replications in this study. Evaluation of the effective Myanmar Bradyrhizobium strains for plant growth, nodulation and N₂ fixation were studied in pot experiments using sterilized vermiculite in the Phytotron (controlled-environmental condition). Then, a pot experiment was conducted using Futsukaichi soil in the screen house (natural environmental condition). The N₂ fixation ability of soybean was evaluated by acetylene reduction activity (ARA) and the relative ureide index method. In the first screening experiment, type A and type B strains with higher nitrogen fixation and proper nodulation on their respective soybean cultivars were selected for the next screening. In the second screening, Bradyrhizobium elkanii AHY3-1 (type A), Bradyrhizobium japonicum SAY3-7 (type A), B. elkanii BLY3-8 (type B) and B. japonicum SAY3-10 (type B) isolates, which showed higher nitrogen fixation and nodulation in Yezin-3 (Rj₄) and Yezin-6 (non-Rj), were selected for the next experiment. In the third screening experiment, SAY3-7 and BLY3-8, which had higher nitrogen fixing potential and proper nodulation, were selected as effective isolates. These two isolates were compatible with non-Rj and Rj₄ soybean varieties for nodulation and nitrogen fixation. Based on the results of the screening experiment, these two strains were tested for their symbiotic efficacy in Futsukaichi soil. This study shows that inoculation treatment of SAY3-7 and BLY3-8 significantly increased plant growth, nodulation, and N₂ fixation at the V6, R3.5 and R8 stages in Yezin-3 (Rj₄) and/or Yezin-6 (non-Rj), and the seed yield at R8 stage, in Yezin-3 (Rj₄) and Yezin-6 (non-Rj) soybean varieties compared with the control treatment. It can be concluded that SAY3-7 and BLY3-8 are suitable for inoculant production because of their higher nitrogen fixation ability, proper nodulation and better productivity of Myanmar soybean cultivars.     

Nitrogen Accumulation in Soybean Following Defoliation

The effects of defoliation on soybean [Glycine max (L.) Merr.] growth and yield have been well studied, but relatively little is known about its nitrogen (N) accumulation after defoliation. An experiment was conducted to examine soybean recovery and N accumulation following defoliation. The indeterminate cultivar (‘Tousan 69’) was planted in a greenhouse, and two defoliation treatments (no defoliation and 67% defoliation) were imposed at the R₂ stage when plants had at least one flower in the two uppermost nodes. At 0, 15, 30 and 45 days after defoliation (DAD), plants were destructively sampled to measure dry mass production, nitrogen accumulation and nitrogen fixation. Seed yield and N concentration also were measured at maturity. Neither the seed yield nor its N concentration was affected by defoliation. Although defoliation temporarily reduced soybean dry weight and N accumulation during 15 DAD, defoliated plants completely recovered their dry weight and N accumulation 30 DAD. There was little difference in N concentration between defoliated and non defoliated plants, indicating that N acquisition was restored during the recovery process. Recovery of N accumulation in defoliated plant was due to complete recovery of N₂-fixing ability and maybe related to improvement in N absorption after defoliation.     

Earthworm contributions to soil nitrogen supply in corn-soybean agroecosystems in Quebec, Canada

Accurately quantifying the soil nitrogen(N) supply in crop fields is essential for enabling environmentally sustainable and economically profitable crop production. It requires using field-based methods to account for the contribution of soil biota, including earthworms, to N mineralization in temperate agroecosystems. The direct contribution of earthworms to the soil N cycle is the N they release throughout their life and after death, and it can be estimated using the secondary production method. This study was conducted in 2014 and 2015 in two adjacent fields with annual corn-soybean rotation in Ste-Anne-de-Bellevue, Quebec, Canada. The cumulative biomass of Aporrectodea spp. in two no-till corn-soybean agroecosystems was determined, and the direct N flux from these earthworms was estimated during the corn and soybean phases of the rotation. Secondary production was estimated by sampling earthworms biweekly during April–June and September–November and inferring the change in earthworm biomass between sampling dates using a size frequency calculation. The N flux was calculated as the sum of the N released through excretion, during periods when earthworms were active, and from mortality. The secondary production of the Aporrectodea population was estimated to be 8–43 g ash-free dry weight m~(-2) year~(-1), and the N flux was 22–105 kg N ha~(-1) year~(-1). The N flux was higher at the early vegetative growth stage, which is a period of high N demand for corn. These findings suggest that refining the N fertilization recommendation by accounting for soil N supplied by earthworms could potentially reduce fertilizer costs and environmental N losses.     

Time-course of dinitrogen fixation of promiscuous soybean cultivars measured by the isotope dilution method

 Soybean cultivars capable of nodulating with indigenous Bradyrhizobium spp. have been developed by the International Institute of Tropical Agriculture (IITA) and national programs in Africa in order to avoid artificial inoculation by resource-poor farmers in Africa. The current selection procedure for enhanced N₂ fixation is based on an assessment of nodule formation which does not directly quantify the proportions of crop N derived from the atmosphere. We have monitored N accumulation patterns and N₂ fixation in nine promiscuous soybean cultivars with different maturity periods, using the ¹⁵N dilution technique. Nodule development generally peaked at the early podfill stage for all cultivars except Tgx 1519-1D and Tgx 1447-2D in which it continued to increase. The proportion of crop N derived from fixation (%NDFA) ranged between 51% and 67%, 77% and 84%, and 66% and 73% at full bloom, early podfill, and physiological maturity stages, respectively. Total N accumulation increased in all soybean genotypes with increasing plant age. Significant correlations (P<0.001) were established between nodule weight and %NDFA, even though this did not explain the relationship between nodule development and N₂ fixation in cultivars such as Tgx 1519-1D. Promiscuous soybean cultivars retained between 10% and 19% of total N accumulated at the final harvest, in belowground biomass. Our results indicated that these soybean cultivars can derive substantial proportions of plant N from N₂ fixation in soils where compatible indigenous bradyrhizobia populations are adequate and effective. Also, we have substantiated the claims that qualitative nodulation parameters currently used to select varieties with a high N₂ fixation capacity need to be validated with other measurements of N₂ fixation. Received: 5 November 1998     

Effect of starter fertilizer in seed-row on emergence,biomass and nutrient uptake by six pulse crops grown under controlled environment conditions

Abstract

Legumes have a unique ability to obtain a significant portion of atmospheric nitrogen (N₂) through a symbiotic relationship with Rhizobia spp of bacteria but it takes time, thus, an early supply of N to the plant may positively influence growth and development. However, too much fertilizer in close proximity to the seed can damage the seedling. Therefore, this study was conducted to determine the maximum safe rates for starter seed-row fertilizer application under low seedbed utilization conditions (15%). Emergence, biomass yield and nitrogen (N), phosphorus (P) and sulfur (S) uptake responses to starter fertilizer products and blends applied at 0, 10, 20 and 30?kg?N?ha^?1 in the seed-row were investigated for six different pulse crops: soybean, pea, faba bean, black bean, lentil and chickpea. The general sensitivity (injury potential) for starter N, P, S fertilizer was lentil?≥?pea?≥?chickpea?>?soybean?≥?black bean?>?faba bean. Lentil, pea and chickpea could generally only tolerate the 10?kg?N?ha^?1 rates while soybean and black bean could tolerate 10–20?kg?N?ha^?1. Faba bean emergence appeared relatively unaffected by all three rates of N and showed least sensitivity to seed row placed fertilizer. In terms of 30-day biomass response, soybean and black bean were most responsive to fertilization, while pea, faba bean, lentil and chickpea were least responsive to the starter fertilizer applications, with no benefit increasing above the 10?kg?N?ha^?1 rate.     

Effects of Rhizobial inoculant and nitrogen fertilizer on yield and nodulation of common bean

A two‐year field experiment was conducted to determine if using mixed strains of Rhizobium inoculant and starter nitrogen (N) fertilizer could improve yield and nodulation of four common bean varieties on a Vertisol at Alemaya, Ethiopia. A granular mixed inoculant of CIAT isolates 384, 274, and 632 and a starter N fertilizer at a rate of 23 kg N ha^‐1 (50 kg urea ha^‐1) were applied separately at planting. Inoculation with mixed strains and starter N fertilizer gave a significantly higher grain yield, nodule number, and dry matter yield for most varieties used. Both grain yield and dry matter yield showed a significant correlation (r=0.93 and r=0.87; P<0.05 for grain yield and dry matter yield, respectively, for 1991 crop season and r=0.90 and r=0.86; P<0.05 for grain yield and dry matter yield, respectively, for 1992 crop season) with nodule number. It is recommended that resource‐poor farmers adopt the practice of using a Rhizobium inoculant or starter N to improve common bean yields in the Hararghe highlands, Ethiopia.