Effect of Salt Stress on Growth and Chlorophyll Content of Some Cultivated Cotton Varieties Grown in Syria

Five upland cotton varieties (Gossypium hirsutum L.) (Aleppo 118, Aleppo 33/1, Aleppo 90, Raqqa 5, and Deir-Ezzor 22) were evaluated under different salinity indices [0, 50, 100, and 200 mM sodium chloride (NaCl)] for 56 days. During the course of the experiment, plant size (HT), leaf number (LN) and leaf area (LA), chlorophyll SPAD (Soil Plant Analyses Development), chlorophyll (Chl) a and b content, and osmotic potential were measured in both control and salt-stressed plants for all tested varieties. Salt-stress application reduced the HT and LN of all varieties, whereas LA decreased as salinity level increased for all tested varieties comapared to their respective control expect for Deir-Ezzor 22 variety. Our data showed that LA, Chl a and b, and chlorophyll SPAD can be used to discriminate between salt-tolerant varieties and salt-sensitive ones. In this respect, Deir-Ezzor 22 variety differed by showing high salt tolerance relative to Aleppo 118 and Aleppo 33/1 varieties. Therefore, this investigation aimed for a better understanding of the different responses presented among these varieties toward salt treatment.     

磁化水对盐胁迫下黄瓜生长和生理特性的影响

为探究磁化水浇灌对盐胁迫下黄瓜幼苗生长和生理特性的影响,采用人工气候室内进行的二因素二水平砂培试验,即2个磁化水水平(D:蒸馏水、M:磁化蒸馏水)和2个盐处理水平(Na0:0 mmol/L,Na1:100 mmol/L NaCl),共4个处理,研究了盐胁迫下磁化水浇灌对黄瓜幼苗生长、水分关系、光合气体交换、抗氧化能力、养分(N,P和K)及Na含量等的影响。结果表明:(1)无论在非盐胁迫和盐胁迫下,浇灌磁化水均具有改善黄瓜幼苗生长及/或生理活动的作用。磁化水在盐胁迫下改善黄瓜生长和生理活动的作用大于非盐胁迫下,且随盐胁迫或磁化水处理时间的延长,磁化水的效应增加。(2)盐胁迫下,磁化水浇灌改善黄瓜幼苗生长的主要生理机制在于:增加了黄瓜幼苗根系生长,改善了叶片水分关系,增加了叶片叶绿素含量、光合速率和水分利用效率,增强了叶保护酶活性从而降低了叶细胞膜受伤害程度,降低了根、茎和叶中的Na含量从而增加K⁺/Na⁺比。研究结果为磁化水用于次生盐渍化土壤上生长的黄瓜灌溉提供了理论依据。     

外源SA、GSH对Cd胁迫下绵毛水苏生理和生长的影响

为探究不同外源物质对Cd胁迫下绵毛水苏生理和生长的缓解效果,采用盆栽试验研究叶面喷施不同浓度水杨酸(SA)(0.5、1.0、1.5、2.0 mmol·L-1)和谷胱甘肽(GSH)(0.1、0.2、0.3、0.4 mmol·L-1)对300 mg·kg-1 Cd胁迫下绵毛水苏幼苗生长、渗透调节物质、抗氧化酶活性以及Cd含...     

外源水杨酸对盐胁迫下棉花幼苗光合作用的影响

为了探究水杨酸(SA)对盐胁迫下棉花幼苗的缓解机理,以2个对盐胁迫敏感程度不同的棉花品种中棉所41号和中棉所49号为材料,研究SA浸种和叶面喷施对盐胁迫下棉花幼苗光合作用的影响。结果表明,0.05、0.06 mmol·L~(-1) SA浸种和0.08、0.12、0.18 mmol·L~(-1) SA叶面喷施均可提高棉花幼苗的株高、干物质积累、叶绿素含量、净光合速率、胞间CO_2浓度和气孔导度,能有效缓解0.6%NaCl的胁迫。中棉所41号在0.05 mmol·L~(-1)SA浸种处理和0.08 mmol·L~(-1) SA叶面喷施处理表现最好,中棉所49号在0.06 mmol·L~(-1) SA浸种处理和0.18 mmol·L~(-1) SA叶面喷施处理表现最好。主成分分析表明,单株鲜重、叶干重、净光合速率和气孔限制值可以作为评价不同浓度的SA对盐胁迫缓解效果的主要指标。综上所述,SA浸种与叶面喷施2种处理方式均可缓解0.6%NaCl带来的伤害,但存在浓度效应,适宜的浓度与棉花的基因型与耐盐性密切相关。本研究为进一步揭示外源SA对棉花盐胁迫的缓解机理提供了参考,同时也为新疆盐碱地棉花种植提供了理论依据。     

盐分胁迫下控释尿素配施腐植酸对棉花幼苗生长和抗氧化系统的影响

为探究盐分胁迫下,控释尿素配施腐植酸对棉花幼苗生长特性和植株体内抗氧化系统的影响,以滨海盐化潮土(全盐含量4.5 g/kg)为供试土壤,设置普通尿素、控释尿素、普通尿素+腐植酸、控释尿素+腐植酸4个施肥处理,以不施氮肥处理为对照进行棉花盆栽试验,测定棉花植株生物产量、叶片叶绿素含量、叶片及根系抗氧化酶(SOD、POD、...     

外源脱落酸对铝胁迫下栝楼叶绿素荧光及生理活性的影响

通过比较不同栝楼品种的耐铝性差异,确定最佳缓解浓度,选取耐铝性有差异的保定栝楼和浦阳栝楼为试验材料,设置6组不同浓度的Al~(3+)和脱落酸,研究Al~(3+)和脱落酸的互作对2个栝楼品种植株的根长、叶绿素荧光特性、根系活力、MDA含量、抗氧化酶活性和抗氧化剂的含量。结果表明:(1)保定栝楼和浦阳栝楼在铝胁迫和外源脱落酸的作用下,各指标体现出相似的变化趋势,但是变化程度和敏感性显露出了一定的区别,保定栝楼具有更强的耐铝能力。(2)铝胁迫使得2个品种栝楼的根长、根系活力、叶绿素荧光参数中的F_v/F_m、F_m和ETR、抗氧化酶SOD、POD、CAT以及抗坏血酸-谷胱甘肽(AsA-GSH)循环系统中AsA、GSH含量都有所抑制,而F_0、MDA和DAsA上升。(3)外源脱落酸加入后,2个品种的抗氧化酶以及AsA、GSH均显现了先上升后下降的趋势,MDA持续降低,F_0和DAsA呈现出由降而升的趋势,其中5mg/L的外源脱落酸使栝楼的根长、根系活力、F_v/F_m、F_m、ETR、SOD、POD、CAT以及AsA、GSH上升到峰值,MDA下降,F_0和DAsA降到谷值。(4)经过隶属函数和耐铝系数的分析,2个品种都能得出相同的结果,铝胁迫下各浓度外源脱落酸对2个品种的缓解程度为5 mg/L7.5 mg/L2.5 mg/L0mg/L10mg/L。保定栝楼的耐铝能力强于浦阳栝楼,低浓度的外源脱落酸可以缓解铝胁迫,5 mg/L浓度的外源脱落酸缓解铝胁迫效果最佳,在生产实际中要选择耐铝性较强的栝楼进行栽培,并辅以适当浓度的外源脱落酸。     

Effect of Exogenous Silicon on Ion Distribution of Tomato Plants Under Salt Stress

Seedlings of two tomato cultivars were exposed to 0, 50, or 100 mM sodium chlroide (NaCl) stress with or without silicon (Si) for 10 days, and leaf electrolyte leakage, root activity, plant growth, and ion sodium, potassium, calcium, and magnesium (Na⁺, K⁺, Ca²⁺, and Mg²⁺) contents were determined. No significant differences were observed in total biomass and the root/crown ratio of salt-stressed plants treated with exogenous Si, but leaf electrolyte leakage of both cultivars treated with 50 mM NaCl and Si was lower than that in the same salt treatment without Si. Root activities of both cultivars were significantly affected by treatment with NaCl and exogenous Si. Application of Si induced a significant decrease in Na⁺ content and increases in K⁺, Ca²⁺, and Mg²⁺ contents in leaves of plants treated with 50 mM NaCl, and consequently the K⁺/Na⁺ and Ca²⁺/Na⁺ ratios increased by at least two times. The effects of Si on the ion contents of the roots were not notable.     

外源水杨酸对干旱胁迫及复水下苎麻生理特性和产量的影响

以中苎2号为研究对象,通过设置干旱胁迫(DS)、1 mmol·L-1水杨酸(SA)喷施+干旱胁迫(DS+SA)、1 mmol·L-1SA喷施+正常浇水(CK+SA)、正常浇水(CK)4个处理,研究干旱胁迫下外源SA对苎麻可溶性蛋白和可溶性糖、叶片和韧皮部相关酶活性等生理机制及其生长发育的影响。结果表明,干旱胁迫第20天时,与DS处理相比,DS+SA处理的苎麻叶片可溶性蛋白和可溶性糖含量显著增加(P0.05),分别提高了20.02%和17.91%。外源SA能明显缓解胁迫植株的氮代谢相关酶(NR、GS、GOGAT和GDH)和纤维发育相关酶(蔗糖合成酶、IAA氧化酶和β-1,3-葡聚糖酶)活性的降低;到干旱胁迫第20天和第30天,DS+SA处理NR活性比DS处理提高了2.02倍和0.73倍,GOGAT活性提高了67.83%和22.90%,而DS+SA处理的GS活性到干旱胁迫第10天和第20天与DS处理差异显著(P0.05)。DS处理的纤维发育相关酶到第30天下降幅度较大,3种酶活性分别只有CK的37.84%、30.71%和35.73%,而DS+SA处理能达到CK的58.23%、74.17%和60.11%。干旱导致苎麻生长缓慢,生物量与CK差异显著(P0.05),经DS+SA处理的植株的产量指标均高于DS处理。因此,在干旱胁迫下,外源水杨酸能够明显缓解植物体内相关酶活性的降低,维持植物正常生长,增强苎麻植株对干旱胁迫的耐受性。本研究为苎麻应对干旱胁迫提供了理论依据。     

冷激结合水杨酸处理对黄瓜果实冷害及能量和脯氨酸代谢的影响

为探讨冷激结合水杨酸处理减轻黄瓜果实冷害的作用及其机理,本试验分别研究了冷激(0℃冰水混合物)、水杨酸(1 mmol·L-1)以及二者结合(含有1 mmol·L-1水杨酸的0℃冰水混合物)3种处理方式对黄瓜果实低温贮藏期间能量和脯氨酸代谢的影响.结果表明,冷激、水杨酸以及二者结合处理均能抑制黄瓜果实冷害程度、相对电导率...     

不同盐胁迫对滴灌棉花生理生长及产量的影响

为探明不同程度轻度盐胁迫对滴灌棉花光合生理、生长指标及产量的影响,采取桶栽的方式人为设置不同土壤含盐量,研究了6种不同盐胁迫(CK:1.5g/kg,T1:3.0g/kg,T2:4.0g/kg,T3:5.3g/kg,T4:6.2g/kg,T5:7.3g/kg)处理下棉花的生理生长及产量的变化规律,为新疆轻度盐碱地棉花栽培管理提供理论基础。结果表明,随着盐胁迫程度的增加及胁迫时间的延长,棉花光合生理指标均呈下降趋势(P0.01)。T1、T2处理对棉花净光合速率(P_n)、蒸腾速率(T_r)、气孔导度(Gs)、细胞间CO_2浓度(Ci)及叶片水分利用效率(WUE)的影响不显著(P0.05),而T3、T4及T5处理对光合生理指标具有显著抑制性(P0.05);生育前期盐分胁迫下P_n与T_r、G_s、C_i均呈下降趋势,而L_s值随着盐胁迫的增加呈升高趋势。吐絮期的P_n、G_s、_Ls均小于蕾期,而C_i却大于蕾期;株高、叶面积在生育前期受到盐胁迫的影响显著,到生育后期T2、T3处理株高、叶面积增长迅速,最大值均为T3处理;T1、T2较CK处理对棉花产量及单株铃数、单铃质量影响不大,T3处理产量迅速降低,通过分段耐盐函数得到棉花在0—40cm土层全生育期的耐盐临界值为5.441 9g/kg(St)、耐盐极限值为44.201 6g/kg(S0);产量与P_n、WUE、株高(H)、叶面积(S)拟合程度较好,相关系数分别为0.69,0.73,0.50和0.72。综合分析,与中、重度盐分胁迫相比,轻度盐胁迫下导致棉花光合速率下降的原因生育前期为气孔限制因素,生育后期为非气孔限制因素,光合参数与盐胁迫程度关系密切,而棉花产量又表现出与光合作用指标具有较好的相关关系。     

种子引发剂氯化胆碱和吲哚丁酸钾对盐胁迫下水稻幼苗生长和生理特性的影响

为探讨种子引发剂对盐胁迫下水稻幼苗生长及生理特性的影响机制,以杂交稻湘两优900和常规稻黄华占为材料,研究引发剂氯化胆碱(CC)和吲哚丁酸钾(IBAK)对盐胁迫下水稻幼苗生长、光合特性、抗氧化代谢及渗透调节的影响。结果表明,盐胁迫抑制水稻幼苗生长,3.0 mg·L^-1CC和1.0 mg·L^-1IBAK为缓解盐胁迫对水稻幼苗生长抑制的适宜引发浓度,添加引发剂CC的湘两优900根干重显著增加9.64%,而添加引发剂CC和IBAK的黄华占根系总长度、叶面积、地上干重、根干重、壮苗指数分别显著增加24.78%和14.39%、43.06%和36.33%、11.45%和7.43%、34.60%和28.62%、41.89%和33.55%。引发剂CC和IBAK处理可显著提高盐胁迫下两品种水稻叶片的净光合速率(Pn)、气孔导度(Gs)、蒸腾速率(Tr)和叶绿素含量;激活超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)和抗坏血酸过氧化物酶(APX)活性;同时增加抗坏血酸(AsA)、谷胱甘肽(GSH)、可溶性蛋白和脯氨酸含量;抑制活性氧积累,降...     

基于数字基因表达谱筛选黄瓜中水杨酸诱导基因

本文通过筛选水杨酸诱导黄瓜叶片差异表达基因(DEGs),旨在揭示水杨酸提高黄瓜抗病性机制和信号转导过程.以抗霜霉病黄瓜东农649为试材,无菌培养幼苗至4叶期,喷施5 mmol·L-1的水杨酸,喷施前和喷施后3、12和24 h分别采取叶片,提取总RNA,制备cDNA文库,应用Illumina高通量测序技术对水杨酸处理前后的4个叶片cDNA文库进行差异基因数字表达谱分析.结果表明,SA诱导了多数与叶绿体和细胞壁相关基因的下调表达和多个与抗病相关基因的上调表达,对受体激酶、细胞色素P450、脂氧合酶和脂转运蛋白等信号转导相关基因的表达均有显著影响.本研究获取了丰富有效的数据,初步筛选的DGEs涉及到植物的PCD(程序性细胞死亡)、信号转导、系统获得性抗性(SAR)形成等过程,证实了SA与上述过程有密切关系,为最终揭示SA提高黄瓜抗病性机制奠定了基础.     

Effects of Exogenous Salicylic Acid on Physiological Characteristics of Peanut Seedlings under Iron-Deficiency Stress

The effects of salicylic acid (SA) on iron (Fe) deficiency in peanut (Arachis hypogaea L.) were studied by adopting the hydroponic experiment. Iron deficiency caused serious chlorosis, inhibited plant growth and dramatically decreased the concentration of Fe in the roots. Furthermore, it decreased the active Fe content and chlorophyll content, and disturbed ionic homeostasis. In addition, Fe deficiency significantly increased the content of malondialdehyde (MDA) and the superoxide anion (O₂^??) generation rate. Addition of SA increased Fe concentration in the shoots and roots, active Fe content, chlorophyll content, the net photosynthetic rate, and transpiration rate. Moreover, SA supplementation alleviated the excess absorption of manganese (Mn), copper (Cu) and zinc (Zn) induced by Fe deficiency. In addition, the chlorosis symptom was alleviated and the plant growth was improved. Meanwhile, addition of SA increased the activities of catalase (CAT) and peroxidase (POD), and decreased the content of MDA and the O₂^?? generation rate. These results suggest that exogenous SA can alleviate Fe-deficiency induced chlorosis by promoting the plant growth, improving the efficiency of Fe uptake, translocation and utilization, protecting antioxidant enzymes system, and stimulating mineral element maintenance.     

外源NO对盐胁迫下甜高粱种子萌发和幼苗生长的影响

为探究外源NO对盐胁迫下甜高粱种子萌发和幼苗生长的影响,以国能4号甜高粱为试验材料,采用不同浓度(0.05、0.1和0.2 mmol·L^-1)硝普钠(SNP,NO供体)处理1.6%NaCl胁迫下的种子和幼苗,统计种子发芽数,测定幼苗叶片叶绿素、脯氨酸、可溶性糖、可溶性蛋白、丙二醛(MDA)含量及超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)和抗坏血酸过氧化物酶(APX)活性等生理生化指标。结果表明,盐胁迫抑制了甜高粱种子的萌发,种子发芽指标(种子发芽率、发芽势和发芽指数)显著降低,1.6%NaCl是甜高粱种子萌发的敏感盐浓度;喷施不同浓度SNP可显著提高盐胁迫下甜高粱种子的发芽率及幼苗叶片叶绿素、可溶性糖、可溶性蛋白和脯氨酸含量,降低叶片MDA含量,增强抗氧化酶活性,其中0.1 mmol·L^-1SNP处理效果最佳。0.1 mmol·L^-1SNP处理下,与单独盐浓度处理相比,甜高粱种子发芽率、发芽势和发芽指数分别增加了29.51%、39.21%和38.91%;幼苗叶片叶绿素a、叶绿素b、总叶绿素、可溶性糖、可溶性蛋白和脯氨酸含量分别提高了230.00%、184.38%、214.13%、17.00%、8.78%和40.63%;MDA含量降低了34.01%;SOD、POD、CAT和APX活性分别增强了33.38%、55.75%、23.17%和116.46%。综上,盐胁迫下适宜浓度的SNP处理,可提高甜高粱幼苗叶片渗透调节物质含量,增强抗氧化酶活性,清除体内活性氧,从而促进幼苗生长,增强植株抗盐性。本研究结果为提高甜高粱耐盐性及揭示其耐盐机制提供了理论依据。     

Influence of Salicylic Acid and Jasmonic Acid on Wheat Under Drought Stress

Salicylic acid and jasmonic acid play an important role in plants coping with abiotic stresses. An experiment was conducted to examine the effect of salicylic acid and jasmonic acid on wheat under drought. Seeds were primed with jasmonic acid (100µM) and salicylic acid (10 Mm). Water stress was applied by withholding water and each treatment was replicated three times with a factorial block design. Application of Salicylic acid and Jasmonic acid mitigated drought effects in wheat. Results revealed that 100µM Jasmonic acid was more effective than 10 mM SA. Drought decreased germination by 26%, whereas application of Jasmonic acid and Salicylic acid ameliorated stress with the increase of germination by 27% and 21%, respectively. An increase in the shoot length of 23% and 20% was observed with Jasmonic acid and Salicylic acid, under drought conditions. The increase in water potential was 60% and 47% with JA and SA while the increase in proline and soluble sugar was 14% and 25% respectively. The application of Jasmonic acid and Salicylic acid has a potential to enhance the growth of wheat plants under drought.     

Effect of Free and Encapsulated Raoultella Planticola Rs-2 on Cotton Growth Promotion Under Salt Stress

The effectiveness of free and encapsulated Raoultella planticola Rs-2 inoculants in promoting cotton growth under salinity stress were investigated. The germination and seedling rates of cotton seeds inoculated with encapsulated Rs-2 (CRs-2-S) were more higher than those of cotton seeds treated with free Rs-2 (FRs-2-S) and non-inoculated strain (CK-S) under salt stress. Meanwhile, the root length, shoot length, and biomass of cotton plants in CRs-2-S increased compared with those of plants in FRs-2-S and CK-S. Moreover, the content of photosynthetic pigments of cotton leaves, peroxidase and superoxide dismutase of cotton seedlings in CRs-2-S treatment were greater than those in FRs-2-S and CK-S treatments. The proline and malendialdehyde contents of cotton seedlings in CRs-2-S decreased compared with FRs-2-S and CK-S-treated seedlings. The nutritional elemental uptake of cotton plants in CRs-2-S treatment was enhanced and the accumulation of sodium decreased compared with FRs-2-S and CK-S plants. Results demonstrate that encapsulated Rs-2 has more potential positive effects on cotton seedlings than free cells.     

盐胁迫对银水牛果幼苗生长和生理特性的影响

通过盆栽试验,采用人为加入NaCl的方法以控制土壤含盐量(0.40%,0.60%,0.80%),研究盐胁迫对2年生银水牛果(Shepherdia argentea)幼苗叶片生长和生理特性的影响,结果表明:(1)随着土壤含盐量的增加,银水牛果幼苗的生长明显受到抑制,株高、生物积累量降低,单株总叶面积、叶片相对含水量减少,净光合速率下降;(2)随胁迫时间的延长,银水牛果幼苗叶片净光合速率在含盐量低于0.60%的土壤中先降低后升高,表明植物幼苗已适应了该盐分的土壤,银水牛果具有较强的耐盐性,本试验条件下以净光合速率下降50%为标准求得银水牛果的耐盐阈值为0.63%;(3)在相同含盐量的土壤中,与对照相比,Ca~(2+)的降幅小于K~+,表明银水牛果幼苗叶片以维持较高的Ca~(2+)含量,保证自身代谢的正常进行,通过提高Mg~(2+)含量以维持自身正常的光合作用,从而适应盐胁迫。     

Effect of Biochemical and Physiological Response to Salt Stress in Camelina sativa

Camelina sativa is a promising low-input, high yielding biodiesel crop that can be suitable to grow on marginal lands and is a good source of omega-3 fatty acids. The objective of the study was to compare the rate of growth in different varieties of C. sativa in response to salt stress. Three biological replicates were collected from three varieties of C. sativa grown in vitro in a controlled growth room. The Blaine Creek, Cheyenne and Suneson varieties of C. sativa were analyzed for their physiological and biochemical response to salt stress using the following criteria: germination rates, measurement of length, dry weights, fresh weights, and stomatal size. The biochemical measurements focused on proline, chlorophyll, and flow cytometry analysis. Blaine Creek’s growth during salt stress was overall the most affected, while Suneson and Cheyenne generated higher amounts of biomass, thus indicating that C. sativa can be grown on saline soils.     

根施5-氨基乙酰丙酸对亚适宜温光下黄瓜幼苗生长的影响

为缓解亚适宜温光环境对黄瓜幼苗生长产生的不利影响,以中农26号为试验材料,研究亚适宜温光下根施不同浓度5-氨基乙酰丙酸(ALA)对黄瓜幼苗生长和生理特性的影响。结果表明,亚适宜温光下,根施5 mg·L^-1 ALA可以显著提高黄瓜幼苗的全株干重、壮苗指数和根系活力,与对照相比,黄瓜幼苗全株干重和壮苗指数分别提高了32.5%和47.1%,根系活力提高了95.0%。根施5 mg·L^-1 ALA能够显著增加幼苗叶片叶绿素和ALA含量,与对照相比,总叶绿素和ALA的含量分别提高了49.5%和55.7%。根施5 mg·L^-1 ALA黄瓜幼苗的净光合速率(Pn)和气孔导度(Gs)分别较对照提高了22.9%和42.2%。根施5 mg·L^-1ALA后,抗氧化酶活性显著提高,叶片和根系的丙二醛(MDA)含量分别比对照降低了35.3%和18.1%。同时,根施适宜浓度的ALA对矿质元素的吸收和运输产生有一定影响,但对叶绿素合成过程中相关基因的表达无明显调控作用。本研究为缓解黄瓜幼苗亚适宜温光伤害提供了新方法,也为ALA在设施农业上的应用提供了技术支持。     

盐胁迫对蒙古黄芪种子萌发与幼苗生长的影响

[目的]分析蒙古黄芪种子在不同浓度NaCl,Na_2SO_4和NaHCO_3处理下的种子萌发和幼苗生长情况,为蒙古黄芪在盐碱地的引种、驯化和种植提供理论依据。[方法]通过发芽盒进行种子萌发出苗试验,测定分析蒙古黄芪种子在不同浓度NaCl,Na_2SO_4和NaHCO_3处理下的萌发率、发芽势、相对盐害率及其苗根长、苗高及根茎比。[结果](1)蒙古黄芪种子在NaCl0.2%,Na_2SO_40.6%和NaHCO_30.3%的盐胁迫下能够正常发芽,萌发率和发芽势均高于对照,在大于此浓度的盐胁迫下萌发率和发芽势均开始下降。(2)蒙古黄芪种子在NaCl0.6%,Na_2SO_40.8%和NaHCO_30.3%时的相对盐害率为负值,促进种子萌发,大于此浓度时,相对盐害率为正值,抑制种子萌发。(3)蒙古黄芪幼苗的茎长、根长及根茎比随着盐浓度的增大而增大,当NaCl和Na_2SO_4浓度分别在0.4%和0.6%时,黄芪幼苗的茎长和根长达最大,NaHCO_3浓度分别在0.6%和0.3%时,黄芪幼苗的茎长和根长达最大,超过此浓度黄芪幼苗的茎长和根长开始减小;当NaCl,Na_2SO_4和NaHCO_3浓度分别为0.6%,0.8%和0.3%时根茎比达最大,分别为1.658,1.709,1.291,超过此浓度,根茎比开始下降,但均大于1。[结论]蒙古黄芪在NaCl0.6%,Na_2SO_40.8%和NaHCO_30.3%的盐胁迫下种子和幼苗能够正常萌发和生长。