Growth and Iron Uptake of Lowland and Aerobic Rice Genotypes under Flooded and Aerobic Cultivation

With increasing water shortages in China, rice (Oryza sativa L.) cultivation is gradually shifting away from continuously flooded conditions to partly or even completely aerobic conditions. The effects of this shift on the growth and iron (Fe) nutrition of different aerobic and lowland rice genotypes are poorly understood. A field experiment was conducted to determine the effects of cultivation system (aerobic vs. flooded), genotype (five aerobic rice varieties and one lowland rice variety), and Fe fertilization [no Fe and 30 kg ha^?1 ferrous sulfate (FeSO₄·7H₂O] on rice grain yield and Fe nutrition. Plants were sampled at tillering and physiological maturity. In both aerobic and flooded plots, Fe application significantly increased shoot dry weight, shoot Fe concentration, and shoot Fe content at tillering but not physiological maturity. At physiological maturity, grain yield and Fe and grain harvest indices were significantly lower in aerobic than in flooded plots. Shoot dry weight and shoot Fe content differed among genotypes at tillering and at physiological maturity. The grain harvest index of aerobic rice genotype 89B-271-17(hun) was significantly greater than that of the other five genotypes when no Fe was applied. Because soil Fe fertilization did not improve the Fe nutrition of rice in aerobic plots, the results indicate that the shift from flooded to aerobic cultivation will increase Fe deficiency in rice and will increase the problem of Fe deficiency in humans who depend on rice for nutrition.     

Impacts of Combination of Foliar Iron and Boron Application on Iron Biofortification and Nutritional Quality of Rice Grain

To increase iron (Fe) concentration in edible portions of staple food crops, an agronomic approach via foliar Fe-containing solutions might be sustainable and economical strategy; however, little information is available in the literature. So the present work was carried out to examine the effects of Fe in association with boron (B) foliar fertilization on Fe biofortification and the nutritional quality of rice (Oryza sativa L.) grain. The work was conducted in 2006 at the research experiment station at Zhejiang University on japonica rice ‘Bing 98110’ planted on a silty loam soil in pots. The following spray treatments were performed at rice anthesis: (1) control (the deionized water spray); (2) 0.1% (w/v) FeSO₄ · 7H₂O; (3) 0.1% (w/v) Fe(II)-AA (Complex of 0.1% FeSO₄ · 7H₂O and 0.4% compound amino acids; 18.6% N); (4) 0.2% (w/v) H₃BO₃ (boric acid, 17.5% B); (5) combined spray of 0.1% (w/v) FeSO₄ · 7H₂O and 0.2% (w/v) H₃BO₃; (6) combined spray of 0.1% (w/v) Fe(II)-AA and 0.2% (w/v) H₃BO₃. Foliar Fe and B complex application did biofortify Fe concentration and other measured nutritive values in polished rice. Compared to the control, Fe concentration in seed increased significantly 18.9% with the combination of foliar Fe(II)-AA and B, Zn content increased significantly 26.7%, and protein and total 16 amino acids, such as lysine, threonine, and arginine that were essential for human nutrition as well as glutamic acid, aspartic acid, valine, leucine, and phenylalanine, etc., also increased markedly by 30.9% and by 37.0%, respectively.     

Protoplast culture utilization in studies on legume crops

Abstract

The available literature on whether or not foliar iron (Fe)-containing solutions can be one of the sustainable and low-cost strategies to increase Fe concentration in edible portions of staple food crops consisted of a few previous studies of Fe in plants, and mainly focused on Fe-deficiency remedies. Our experiment was carried out to examine the effects of foliar Fe-containing solutions on Fe enrichment and on the nutritional, cooking, and eating qualities of polished rice of the japonica ‘Bing 98110’ planted on a powdery loam soil under pot conditions. The results showed that Fe concentration in polished rice could be enriched with foliar Fe(II)-amino acids [the main formulation was the complex of 0.1% (w/v) FeSO₄ ^?7H₂O and 0.4% (w/v) compound amino acids, 18.6% N] application. Compared with the control, Fe concentration increased significantly, by 88.0%. Meanwhile, the positive effects on Zn concentration and protein and amino acid content improvement were found with boric acid (H₃BO₃, B) added to Fe(II)-amino acids foliar application. In detail, Zn concentration significantly increased (19.6%), and protein and lysine were increased significantly by 30.1 and 35.1%, respectively. Also the cooking and eating qualities were improved with foliar Fe(II)-amino acids and B compound spray.     

Agronomic evaluation of mulching and iron nutrition on productivity,nutrient uptake,iron use efficiency and economics of aerobic rice-wheat cropping system

Field experiments were carried out during rainy (kharif) and winter (rabi) seasons (June–April) of 2008–2010 at Indian Agricultural Research Institute (IARI), New Delhi, to study the productivity, nutrients uptake, iron (Fe) use-efficiency and economics of aerobic rice-wheat cropping system as influenced by mulching and Fe nutrition. The highest yield attributes, grain and straw yields (5.41 tonnes ha^?1 and 6.56 tonnes ha^?1, respectively) and nutrient uptake in rice was recorded with transplanted and puddled rice (TPR) followed by aerobic rice with Sesbania aculeata mulch. However, residual effect of aerobic rice with wheat straw mulch was more pronounced on yield attributes, grain and straw yields (4.20 and 6.70 tonnes ha^?1, respectively) and nutrient uptake in succeeding wheat and remained at par with aerobic rice with Sesbania mulch. Application of iron sulfate (FeSO₄) at 50 kg ha^?1 + 2 foliar sprays of 2% FeSO₄ was found to be the best in terms of all the yield attributes, grain and straw yield (5.09 and 6.17 tonnes ha^?1, respectively) and nutrient uptake and remained at par with 3 foliar sprays of 2% FeSO₄. Although residual effect of iron application failed to increase the yield attributes, yield and nutrient uptake nitrogen, phosphorus and potassium (N, P, K) except Fe. The highest system productivity, nutrient uptake, gross returns, net returns, B: C ratio and lowest cost of cultivation were recorded with aerobic rice with wheat straw and Sesbania aculeata mulch. Application of FeSO₄ at 50 kg ha^?1 + two foliar sprays of 2% FeSO₄ was found better in respect of system productivity, nutrient uptake, gross returns, net returns, B:C ratio and cost of cultivation in aerobic rice-wheat cropping system. The Fe use efficiency values viz. partial factor productivity (kg grain kg^?1 Fe), agronomic efficiency (kg grain increased kg^?1 Fe applied), agrophysiological efficiency (kg grain kg^?1 Fe uptake), physiological efficiency (kg biomass kg^?1 Fe uptake), apparent recovery (%) utilization efficiency and harvest index (%) of applied Fe were significantly affected due to methods of rice production and various Fe nutrition treatments in aerobic rice and aerobic rice-wheat cropping system.     

Tropical Rice Cultivars from Lowland and Upland Cropping Systems Differ in Iron Plaque Formation

In iron toxic wetlands, ferric hydroxide is commonly deposited on rice roots. This study aims to to evaluate the differences in iron plaque formation in rice cultivars from different cropping systems. Thirty days old seedlings of Brazilian rice cultivars from the lowland cropping system (‘BRS Atalanta’ and ‘Epagri 107’) and upland cropping system (‘Canastra’) or both systems (‘BRSMG Curinga’) and the cultivar ‘Nipponbare’ were exposed to iron excess [4 mM iron sulfate heptahydrate (FeSO₄.7H₂O)] for seven days in nutrient solution. It was observed iron plaque formation and ruptures of the root epidermal cells. The lowland cultivars showed higher Fe content in iron plaque. Iron stain was detected in the root hairs, epidermis, hypodermis, and exodermis. The root exodermis may be contributed to prevent the deposit of iron in the cortex of the lowland cultivars and in the cultivar ‘BRSMG Curinga’. It was observed in plants with iron plaque formation significant reductions in the shoot content of phosphorous, manganese and magnesium due to different causes. The differences in iron plaque formation among the cultivars might be an indicative of variations in exodermis selectivity, root oxidative capacity, and iron nutrition mechanisms.     

Response of date palm to iron fertilization by trunk injection and soil application

To study the response of date palm trees to Fe fertilization, two date palm cultivars, Khlas and Ruzaiz, were fertilized with different levels of FeEDDHA (trunk injected and through soil) and FeSO₄ · 7H₂O(trunk injected). Leaves were collected 100 days after iron application and analyzed for micronutrients. The injection with 100 g FeSO₄ · 7H₂O/tree increased the Fe contents in the leaves of both cultivars, and with 100 g FeEDDHA/tree in the leaves of Ruzaiz cultivar. Soil application of FeEDDHA was not effective. The contents of Cu, Mn and Zn in the leaves were not affected by Fe fertilization.     

Ratios of Nutrients in Lowland Rice Grown on Two Iron Toxic Soils in Nigeria

ABSTRACT

Iron (Fe) toxicity is a widespread nutritional soil constraint affecting rice production in the wetland soils of West Africa. Critical levels of total iron in plant causing toxicity is difficult to determine as different rice cultivars respond to excessive Fe^{2 +} in various ways in what is called “bronzing” or “yellowing” symptoms (VBS). An investigation was conducted to evaluate the relationship between plant growth and nutrient ratios at four iron levels (1000, 3000, 4000 μ g L^?1) and control. This involved two rice cultivars (‘ITA 212’ and ‘Suakoko 8’), and two soil types (Aeric Fluvaquent and Aeric Tropaquept). The experimental design was a 2 × 2 × 4 factorial in a completely randomized fashion with four replications. The results showed that nutrient ratios [phosphorus (P)/Fe, potassium (K)/Fe, calcium (Ca)/Fe, magnesium (Mg)/Fe, and manganese (Mn)/Fe), Fe content, and Fe uptake vary widely with the iron levels as well as with the age of the cultivars. The iron toxicity scores expressed as VBS increased with increasing Fe^{2 +} in the soils, resulting in simultaneous reduction of the following variables: plant height, tiller numbers/pot, relationships grain yield (GY) and dry matter yield (DMY). There were no significant difference between nutrient ratios, Fe contents, Fe uptake, the GY and DMY of both rice cultivars on both soil types. Multiple stepwise regression analysis showed that Fe uptake and Fe contents contributed 42% and 17% respectively to the variation in the grain yield of ‘ITA 212’ on Aeric Tropaquept. On both soil types and cultivars, Fe uptake and Fe content contributed between 26 and 68% to the variation in the DMY, while the nutrient ratios (P/Fe, K/Fe, Ca/Fe, and Mn/Fe) contributed between 3% and 13% DMY. Thus, it could be concluded that iron toxicity in rice is more a function of a single nutrient (Fe) rather than nutrient ratios.     

ZINC-COATED UREA IMPROVES PRODUCTIVITY AND QUALITY OF BASMATI RICE (ORYZA SATIVA L.) UNDER ZINC STRESS CONDITION

A field study conducted for two years (2006 and 2007) at the Research Farm of the Indian Agricultural Research Institute, New Delhi, India showed that zinc (Zn) fertilization increased yield attributes, grain and straw yield, enhanced Zn concentrations and its uptake and improved kernel quality before and after cooking in basmati rice ‘Pusa Sugandh 5’. A 2% Zn-coating with zinc sulfate (ZnSO₄·7H₂O) was found to be the best but a 2% Zn-coating with zinc oxide (ZnO) was very close to it in terms of grain and straw yield and Zn concentrations in basmati rice grain and straw under Zn stress conditions. Partial factor productivity (PFP) of applied Zn varied from 984–3,387 kg grain kg Zn^?1, agronomic efficiency (AE) varied from 212–311 kg grain kg^?1 Zn (applied) and physiological efficiency (PE) of Zn varied from 6,384–17,077 kg grain kg^?1 Zn (absorbed). Thus, adequate Zn fertilization of basmati rice can lead to higher grain yield and Zn-denser grains with improved cooking quality in basmati rices under Zn stress soil conditions.     

Nitrogen‐Supplying Capacity of Soils for Rice and Wheat and Nitrogen Availability Indices in Soils of Northwest India

Abstract

Quantitative assessment of soil nitrogen (N) that will become available is important for determining fertilizer needs of crops. Nitrogen‐supplying capacity of soil to rice and wheat was quantified by establishing zero‐N plots at on‐farm locations to which all nutrients except N were adequately supplied. Nitrogen uptake in zero‐N plots ranged from 41.4 to 110.3 kg N ha^?1 for rice and 33.7 to 123.4 kg N ha^?1 for wheat. Availability of soil N was also studied using oxidative, hydrolytic, and autoclaving indices, salt‐extraction indices, light‐absorption indices, and aerobic and anaerobic incubation indices. These were correlated with yield and N uptake by rice and wheat in zero‐N plots. Nitrogen extracted by alkaline KMnO₄ and phosphate borate buffer and nitrogen mineralized under aerobic incubation were satisfactory indices of soil N supply. For rice, 2 M KCl and alkaline KMnO₄ were the best N‐availability indices. Thus, alkaline KMnO₄ should prove a quick and reliable indicator of indigenous soil N supply in soils under a rice–wheat cropping system.     

IRON STRESS INDUCES PRIMARY AND SECONDARY MICRONUTRIENT STRESSES IN HIGH YIELDING TROPICAL RICE

Two indica rice (Oryza sativa L.) cultivars, viz. ‘Swarna’ and ‘Kalinga III’ were compared for their response to iron (Fe) stress. The cultivars were raised with four Fe levels viz. 0.05, 1, 5, 10 mg L^?1 in hydroponic culture. Plant growth, soluble protein, chlorophyll content and phytoferritin of leaves increased significantly with increase in Fe concentration up to 5 mg L^?1, but decreased at 10 mg L^?1. In contrast, lipid peroxidation, decreased up to 5 mg L^?1 then increased at 10 mg L^?1. However, at 10 mg L^?1 of Fe these parameters were more adversely affected in ‘Swarna’ than ‘Kalinga III’. The later also accumulated relatively more Fe, zinc (Zn), manganese (Mn), and copper (Cu) from the growing medium. Zinc concentrations of the tissue, on the other hand, exhibited the opposite trend. Iron stress may, thus lead to secondary metallic ion stresses and under such situations cultivars like ‘Kalinga III’ will perform better than ‘Swarna’.     

Effect of Cadmium and Iron on Rice (Oryza Sativa L.) Plant in Chelator-Buffered Nutrient Solution

ABSTRACT

To better understand the mechanisms responsible for differences in uptake and distribution of cadmium (Cd), nutrient-solution experiments were conducted with different varieties of rice (Oryza sativa), ‘Khitish’ and ‘CNRH3’. The plants were grown in a complete nutrient solution with different levels of pCd (-log free Cd⁺² activity) and pFe [-log free iron (Fe⁺²) activity]. The required concentrations of chelating agent and metals were determined using a computerized chemical equilibrium model such as Geochem-PC. Experimental treatments included a combination of four pCd activity levels (0, 7.9, 8.2, and 8.5) applied as Cd (NO₃)₂ 4H₂O, and two pFe activity levels (17.0 and 17.8) applied as FeCl₃. The application of both Cd and Fe in solution culture significantly affected plant growth, yield, and Cd accumulation in plant tissue. In general, yield of rice was decreased by an increase in amount of solution Cd; however, yield response varied among the cultivars. At the 7.9 pCd level, yields of rice cultivars ‘Khitish’ and ‘CNRH3’ were reduced to 69% and 65%, respectively, compared with control plants. Root Cd concentrations ranged from 2.6 mg kg^?1 (control plants) to 505.7 mg kg^?1 and were directly related to solution Cd concentrations. In rice plants, Cd toxicity symptoms resembled Fe chlorosis. Differential tolerance of varieties to phytotoxicity was not readily visible, but a significant interaction of substrate Cd and variety was obtained from dry-matter yields. Significant interactions indicated that response of tissue Cd concentration, plant Cd uptake, and translocation of Cd to the aerial parts were dependent on variety as well as substrate Cd. Uptake of Cd by roots was significantly higher than by shoots. Higher Cd uptake by rice plants decreased the uptake of other beneficial metals.

The effect of Cd and Fe on the rate of phytometallophore release was also studied in the nutrient solution. Among the rice genotypes, ‘Khitish’ was the most sensitive to Cd toxicity. In both genotypes, with the onset of visual Cd-toxicity symptoms, the release of phytometallophore (PM) was enhanced. Among the rice varieties, ‘Khitish’ had the highest rate of PM release. Treatments with the metal ions studied produced a decrease in chlorophyll and enzyme activity. A decrease in concentrations of chlorophyll pigments in the third leaf was observed due to the highest activity level of Cd (pCd 7.9). Activities of enzymes such as peroxidase (POD) and superoxide dismutase (SOD) are altered by toxic amounts of Cd. Changes in enzyme activities occurred at the lowest activity of Cd (pCd 8.5) in solution. Peroxidase activity increased in the third leaf. Results showed that in contrast with growth parameters, the measurements of enzyme activities may be included as early biomarkers in a plant bioassay to assess the phytotoxicity of Cd-contaminated solution on rice plants. Evidence that Cd uptake and translocation are genetically controlled warrants the selection of varieties that assimilate the least Cd and that translocate the least metal to the plant part to be used for human and animal consumption.     

Effect of long-term balanced and imbalanced inorganic fertilizer and FYM application on chemical fraction of DTPA-extractable micronutrients and yields under rice–wheat cropping system in mollisols

The imbalanced use of chemical fertilizers under intensive cultivation practices over a period of years leads to various soil-associated problems particularly nutrient availability. Thus, to examine the effect of long-term application of balanced and imbalanced inorganic fertilizer and farm yard manure (FYM) application on the chemical fraction of DTPA-extractable micronutrients under rice–wheat cropping system after 29 years, the observations were recorded from the ongoing field experiment at Govind Ballabh Pant University of Agriculture and Technology, Pantnagar, India. An application of balanced inorganic fertilizer with FYM in rice, while without FYM in wheat significantly improved the DTPA-extractable Zn, Fe, Mn and Cu after rice and wheat crops in both the surface and sub-surface soil layers. Lowest DTPA-extractable Zn, Fe, Mn and Cu were recorded, in surface and sub-surface soil under rice and wheat crops in control. The highest DTPA-extractable Zn, in both surface and sub-surface layers of rice (3.31, 1.62 mg kg⁻¹, respectively) and wheat (2.96, 0.99 mg kg⁻¹, respectively) was recorded because of application of N₁₈₀+P₈₀+K₄₀+Zn(F) + FYM in rice and N₁₈₀+P₈₀+K₄₀+Zn(F) in wheat. However, the DTPA-extractable Fe, Mn and Cu were highest in rice and wheat because of N₁₂₀+P₄₀+K₄₀+FYM and N₁₂₀+P₄₀+K₄₀ application, respectively. The balanced use of inorganic fertilizer with FYM (N₁₈₀+P₈₀+K₄₀+Zn(F) + FYM) in rice and without FYM [N₁₈₀+P₈₀+K₄₀+Zn(F)] in wheat supported the highest rice (6.74 t ha⁻¹) and wheat (3.50 t ha⁻¹) grain yields, while lowest in control. Based on the study results, long-term application of FYM at 5 tonnes ha⁻¹ in rice crop sustained the availability of DTPA-extractable cationic micronutrients to rice and wheat in Mollisols.     

Soil Application of Boron Improves the Tillering,Leaf Elongation,Panicle Fertility,Yield and its Grain Enrichment in Fine-Grain Aromatic Rice

Boron (B) is one of the essential micronutrients having a specific role, particularly during reproductive phase, in rice. In a previous experiment on aerobic rice, panicle sterility was noted as one of the major challenges. This experiment was conducted to evaluate the influence of soil-applied B on tillering, panicle sterility, water relations, and grain enrichment in fine-grain aromatic rice cultivars ‘Super Basmati’ and ‘Shaheen Basmati’. Boron was soil applied at 0.50, 0.75, 1, 1.25, and 1.50 kg ha^?1 while the control treatment did not receive B. Rate of leaf emergence and elongation and tiller appearance were significantly improved by B application. Likewise, B application also improved the leaf chlorophyll contents and water relations in both rice cultivars. Substantial improvement in kernel yield and yield contributing traits was also observed by B application owing to decrease in panicle sterility. A linear increase in leaf and kernel B contents was observed with increase in B application rate. However, the range for an optimum B application rate is very narrow and increase of B application beyond 1 kg ha^?1 was toxic. In conclusion, soil application of B is an effective way to decrease panicle sterility and increase the kernel yield and grain B enrichment in rice.     

Control of arsenic toxicity in rice plants grown on an arsenic‐polluted paddy soil

Abstract

Paddy soils of over 500 hectares had been polluted by arsenic (As) from tailings at an abandoned lead‐zinc mine at Shaoxing, Zhejiang, China. Several field experiments were conducted to establish measures for reducing As toxicity to rice plants. The results obtained were as follows. Fresh Chinese milkvetch (Astragalus sinicus L.) was not supposed to be used as green manure in arsenic polluted paddy soils. Although liming (1,500 kg CaO hectare^‐1) could reduce water‐soluble As (H₂O‐As) in the soil, the rice plant grew badly. The treatments of FeCl₃ (25 mg Fe kg^‐1 soil) and MnO₂ (25 mg Mn kg^‐1 soil) could markedly lower the H₂O‐As and arsenite [As(III)] percentage in the soil and make the plant grow better than the control experiment (CK). Without adding any materials to the soil, wetting and drying (furrowing and draining) in the paddy soil could increase soil redox potential greatly and lower the H₂O‐As and As(III) percentage obviously leading to better rice growth. In addition, the As contents of roots, flag leaf, grain, and husked rice of 11 new cultivare of early rice were determined and correlation analysis was conducted. Uptake and accumulation of As in different parts of cultivars Zhefu‐802 and Erjiufeng at the 4 As levels of the paddy soil demonstrated that the As contents in husked rice of both cultivars exceeded the hygienic standard (0.7 mg As kg^‐1) when they grew in the paddy soil having total As content of about 70 mg kg^‐1 for Zhefu‐802 and 100 mg kg^‐1 for Erjiufeng, respectively.     

Bicarbonate‐induced iron chlorosis in java citronella

Abstract

A field experiment was conducted during 1989–91 to investigate and resolve iron (Fe) chlorosis that appears in the summer season in Java citronella (Cymbopogon winterianus Jowitt). The experiment consisted of nitrogen (N) levels of 0, 200, and 400 kg N/ha/year as main plots and foliar applications of ferrous sulfate (FeSO₄‐7H₂O) (nil and spray once in two months) as subplots in a split‐plot design. A foliar application of a 3% FeSO₄‐7H₂O solution once in two months resulted in the disappearance of the Fe‐deficiency symptoms, increased the leaf chlorophyll content, and the total and ferrous‐Fe contents in the leaf tissue. At the 400 kg N/ha/year treatment level, the foliar Fe spray increased significantly herb and oil yields. Thus, under intensive cultivation of Java citronella, a foliar application of Fe will avoid economic losses of essential oil yields when Fe‐deficiency symptoms appear.     

Effect of Integrated Nutrient Management on Soil Fertility and Yield of Rice (Oryza sativa L.) under the System of Rice Intensification in the Indian Subtropics

Field experiments were conducted at Water Management Research Station, Begopara, Nadia, WB, India, during the rabi seasons of 2008–2009 and 2009–2010 to find out the integrated effect of nitrogen (N), phosphorus (P), potassium (K), farmyard manure (FYM) and zinc (Zn) under the system of rice intensification (SRI) techniques using eight treatments on the fertility changes in soil. The results revealed that the amounts of organic carbon and available N content in soil were found to maintain the highest fertility status with the highest yield in T₆ (NPK + FYM 10 tha^?1 + Zn 5 kgha^?1) and gave the highest N uptake (55.98 kgha^?1). The availability of P decreased with the increased level of Zn application and gave the highest P uptake (23.52 kgha^?1) in the treatment T₅ (NPK + FYM 10 tha^?1). The highest Zn content (4.71 mgkg^?1) was recorded in the treatment T₇ (NPK + FYM 10 tha^?1+ Zn 10 kgha^?1).     

Effects of excess soil manganese on stomatal function in two soybean cultivars

Manganese tolerant ‘Lee’ and Mn sensitive ‘Forrest’ soybean cultivars were grown in a potting soil with no known Mn toxicity and in Loring soil treated with excess Mn. Manganese toxicity in Loring soil was induced by the addition of Mn at 0, 100, 200 and 400 ug g^‐1 as MnSO₄.H₂O. A preliminary experiment was conducted to determine the appropriate Mn stress levels for Lee and Forrest soybean cultivars in Loring soil. Because the Loring soil produced severe Mn toxicity in both cultivars, even with an intial pH of 4.9 and no added Mn, CaCO₃ (2 g kg^‐1 ) was added to Increase the pH to 6–6.3. Soil was analysed for extractable and water soluble Mn and plants for Mn, Ca and Fe.

A second experiment was conducted to determine the effect of Mn toxicity on stomatal function. The procedure was the same as in the first experiment except that the CaCO₃ treatment was 2.5 g kg^‐1 to raise soil pH to 6.2 ‐6.5. Plants were grown in a greenhouse for 10 days and then moved to a growth chamber before making stomatal conductance measurements. A steady state porometer (LI 1600) was used. Results indicated that Mn toxicity closed stomates and decreased transpiration rates. This effect was more pronounced in Mn sensitive Forrest than in Mn tolerant Lee.     

Effects of Fertilizers on Yield,Sustainability, and Soil Fertility under Rainfed Pigeon pea + Rice System in Subhumid Oxisol Soils

A long-term field experiment was conducted at the research farm of the All-India Coordinated Research Project for Dryland Agriculture, Phulbani, Orissa, India, from 2001 to 2006 to identify the best integrated nutrient-use treatments for ensuring greater productivity, profitability, sustainability, and improved soil quality in pigeon pea + rice (two rows of pigeon pea followed by five rows of rice alternately) intercropping system. In all, nine treatments, eight comprising integrated nutrient-use practices, chemical fertilizer (CF), farmyard manure (FYM), and green leaf manure (GLM) to supply nitrogen (N) at 45 kg N ha^–1 and one farmer's practice equivalent to 25 kg N ha^–1 (FYM 5 t ha^–1), were tested on a long-term basis. Results of the study revealed that 20 kg N ha^–1 (FYM) + 25 kg N (CF) gave maximum mean rice grain yield of 1.52 t ha^–1, followed by 20 kg N (GLM) + 25 kg N (urea) with grain yield of 1.51 t ha^–1. In the case of pigeon pea, 30 kg N (FYM) +15 kg N (urea) gave maximum pigeon pea grain yield of 0.94 t ha^–1, which was 34% greater than the sole application of chemical fertilizer. Pigeon pea grain yield tended to increase with increasing proportion of organic N in FYM + CF or GLM + CF combinations. Application of 20 kg N (FYM) + 25 kg N (urea) recorded maximum mean rice equivalent yield of 3.59 t ha^–1 and sustainability yield index of 59%. While studying profitability, application of 20 kg N (FYM) + 25 kg N (CF) gave maximum net returns of US$168.94 ha^–1. Impact of treatments on soil quality as assessed in terms of relative soil quality indices (RSQI) increased with increasing proportion of organic sources of N. Using an innovative and new approach, an index of integrated productivity–sustainability–profitability–soil quality performance index (I _P,S,Pr,SQ) was computed to make a precise evaluation of the treatments. Based on this index, the order of performance of the treatments was T₆ [20 N (FYM) + 25 N (CF)] (7.7) > T₇ [30 N (FYM) + 15 N (CF) (6.9)] > T₃ [20 N (GL) + 25 N (CF)] (6.8) > T₅ [10 N (FYM) + 35 N (CF) (6.6)] > T₉ [GL] (6.5) > T₈ [CF] (6.2) > T₄ [30 N (GL) + 15 N (CF)] (6.0) > T₂ [10 N (GL) + 35 N (CF)] (5.7) > T₁ [FYM at 5 t ha^–1] (4.1). Thus, the results and the methodology adopted in this study using long-term data would be very useful to researchers, farmers, land managers, and other stakeholders not only in India but also across the world under similar climatic and edaphic situations.     

Losses of iron in leachates from organic components of potting media

Abstract

Losses over 18 weeks of iron (Fe) in leachates from several peats and wood wastes that had been amended with ferrous sulfate (FeSO₄‐7H₂O) were generally less than 1% of the total Fe in the material. Increasing additions of Fe either had little effect on Fe losses in leachates or lowered them, mainly through a lowering in the concentration of Fe‐organic complexes in leachates. DTPA‐extractable Fe in wood wastes did not decline with leaching but there were some reductions in peats. The data suggest that a single pre‐plant addition of 0.75–1.0 g/L FeSO₄‐7H₂O would supply enough Fe for at least six months of plant growth in media based on peat with a low native Fe content.     

石灰性紫色土施铁肥与接种根瘤菌对花生-根瘤菌共生固氮作用的影响

以缺铁的石灰性紫色土为供试土壤进行盆栽实验,选用三株慢生型花生根瘤菌Spr3 5、Spr3 7、Spr4 5及gusA和celB标记的菌株gusA3 5、gusA3 7、gusA4 5、celB3 5、celB3 7、celB4 5接种天府9号花生。通过标记根瘤菌形成的根瘤能与检测试剂产生颜色反应的特征,检测施铁肥及施不同浓度的铁肥对花生 根瘤菌有效性和竞争性的影响。结果发现:缺铁的石灰性紫色土上单施铁肥、单接种根瘤菌、接种根瘤菌配施铁肥均能促进花生与根瘤菌的共生固氮效应和竞争结瘤能力,但接种根瘤菌配施铁肥的效果最好,单接种根瘤菌的效果次之,单施铁肥的效果差。喷施0 .2 %硫酸亚铁溶液的效果比0 .3%的好。植株全氮含量和叶绿素含量都是指示共生固氮效应的重要指标,与花生产量间存在极显著的相关性,相关系数分别为0 .76 3和0 .795。gusA和celB两种标记方法检测的结果基本一致,两种标记根瘤菌的平均占瘤率分别为79.6 4 %、75 .6 2 %、74 .4 1%。供试菌株中Spr4 5的有效性和竞争性最强,Spr3 7次之,Spr3 5最差