Establishment of Chlorophyll Meter Measurements to Manage Crop Nitrogen Status in Potato Crop

Management of nitrogen (N) use is necessary to promote maximum development and production of tubers. For this reason, chlorophyll meters have been used to make N-fertilizer recommendations in potato. An assay was carried out with increasing doses of N. To define the thresholds IV for potato crops, correlations among IV, ISN, RR, and N content had been established. IV is the green index of the leaf measured by the chlorophyll meter. This index estimates the chlorophyll content in the leaf and through this the N concentration in vegetal tissue, based on the assumption that N and chlorophyll content have a strong correlation. ISN is the sufficiency nitrogen index. It was determined as the relation between the green index reading of each experimental unit and the highest average green index reading of the assay. RR is the relative yield. It was calculated as the ratio between the yield of each treatment and the highest average yield of the assay. The results had shown that varieties did not present N deficiencies during the vegetative growth phase, with IV and ISN values respectively equal to 41 SPAD units and 93 percent. During the tuber bulking phase, the necessary IV and ISN thresholds to reach maximum yields, for N content equal to 3 percent, were, respectively, between 38.5 and 40.5 SPAD units and between 84 and 94.8 at 70 and 89 days after planting. We concluded that the chlorophyll meter is an appropriate tool to determine the nutritional status in potato crops.     

Nutrients Accumulation,Biometric, Chlorophyll and Potassium Indexes and Production in Minituber Potato as Function of Potassium Doses in Organic and Hydroponic Conditions

ABSTRACT

Potato is one of the most economically important crops in Brazil. In this crop, potassium (K) is the most accumulated nutrient and has significant and positive effect on potato plant growth and tuber yield. Thus, the aim of this work was to determine the effects of K doses on nutrients accumulation, biometric, chlorophyll, and K indexes in potato plants grown. Two experiments were installed simultaneously in an unheated greenhouse with Agata cultivar. The experiment 1 was carried out in pot with commercial organic substrate with five K doses (0, 0.66, 1.32, 1.98, and 2.64 g dm^3?1). The experiment 2, nominated hydroponic system, with washed sand above a layer of expanded clay pebbles where the plants received daily nutrient solution application. The treatments were five K doses (0.0, 2.5, 5.0, 7.5 e 10.0 mmol L^?1). The biometrics data were collected 21 days after emergence and at harvest. Both experiments were set at a randomized block design with four replications. In both experiments, no visual symptoms of deficiency or excess were observed but only in plants at 0 mmol L^?1 of K. In both experiments, K doses interacted with some biometric and chlorophyll indexes measured in the fourth completely expanded leaf with portable SPAD and Dualex devices, commonly used as a guide to in-season fertilizer N management, leading to recommend their use only in plants properly nourished in K. K critical concentration in the fourth fully expanded leaf was 61.7 g kg^?1.     

Nitrogen and Potassium Fertilization Responses of Potato (Solanum tuberosum) cv. Spunta

A potato field experiment was conducted for 2 consecutive years to determine the effects of nitrogen (N) and potassium (K) fertilization rates on the yield and quality of potato cv. Spunta cultivated on soil low in N and K. A 3?×?4 complete factorial experiment was used with three rates of nitrogen (330, 495, and 660 kg N ha^–1) and four rates of potassium (112, 225, 450, and 675 kg K₂Ο ha^–1). An additional treatment without fertilization was used as the control. On soils low in N and K, potatoes showed low yield response to K fertilizer. The greatest tuber yields for both years were achieved at 495 kg N ha^–1 and 112 kg K₂O ha^–1 (29.81 t ha^–1) and 225 kg ha^–1 (27.13 t ha^–1), respectively. Differences in mean fresh weight due to treatment application were not significant. Application of 495 kg N ha^–1 significantly reduced harvest index (the ratio of tuber dry weight to the total dry weight at harvest) compared to 330 kg N ha^–1, but at 660 kg N ha^–1 harvest index achieved the greatest significant value. Potassium fertilization had no significant influence on harvest index. Nitrogen rates positively influenced the number of tubers. The addition of 450 kg K₂O ha^–1 significantly enhanced the number of tubers compared to the lower K rates, and the number was significantly decreased by the application of 675 kg K₂O ha^–1. Tuber dry-matter concentration was significantly promoted by N fertilization in both cultivation years, but it was negatively affected by K fertilization in the first year of cultivation. There was no change in tuber N with N application, but N application strongly increased nitrate (NO₃) concentration, which fluctuated between 360 and 1382 mg kg^–1 wet mass. Tuber NO₃ was negatively correlated with tuber yield, indicating that high levels of NO₃ in tubers can adversely affect yield. Tuber response to K fertilization was not in accordance with the rate of applied nutrient.     

Effect of External Nitrogen and Potassium Supply on Xylem Sap Composition of Sugarcane

ABSTRACT

This study was conducted to evaluate the effect of nitrogen (N) and potassium (K) availability on root exudate composition of two sugarcane cultivars known to differ with regard to their resistance to drought and salinity stress. The plants were hydroponically grown in a greenhouse and subjected to three levels of N (0.1, 1.0, and 10 mM N) and three levels of K (0.02, 0.2, and 2 mM K). Nitrogen and K stress altered the xylem sap composition. Nitrogen stress significantly reduced nitrate (NO₃ ^?), ammonium (NH₄ ⁺), calcium (Ca), magnesium (Mg), and amino acid content and increased the pH, phosphorus (P), and K content. Whereas, K stress significantly decreased pH, K, NH₄ ⁺, and amino acid content but increased Ca, Mg, and P content. Nitrogen and K stress had opposing effects on xylem sap pH and osmolality. Results indicated that sugarcane plants recycle compounds between the phloem and xylem. The results also suggested that the NO₃ ^? and K concentration of xylem sap could be effectively used to estimate the N and K status of the soil solution.     

不同用量包膜氯化钾对马铃薯产量、品质及土壤钾供应的影响

利用马铃薯盆栽试验,通过设置不施钾肥、普通氯化钾、30%、50%、70%以及全量(等量于普通氯化钾)包膜氯化钾的处理,研究不同用量包膜氯化钾对马铃薯产量、品质及土壤钾素供应的影响。结果表明:包膜氯化钾25℃静水浸泡的钾素日均释放量约为1.2%,符合控释肥行业标准,在土埋下的钾素释放率呈S型,于70~90天释放率达到最大值,与马铃薯块茎形成及膨大时期相吻合,有利于产量形成;较普通氯化钾,包膜氯化钾的马铃薯增产幅度为13.92%~36.54%;包膜氯化钾全量处理的Vc含量、可溶性蛋白及淀粉含量显著高于普通氯化钾,而包膜氯化钾全量施用的马铃薯还原性糖含量显著低于其他处理,降低幅度为13.9%~36.2%;包膜氯化钾钾素缓慢释放,满足植株后期对钾的需求,在块茎形成期后,包膜氯化钾50%以上用量处理的植株吸钾量显著高于其他处理;包膜氯化钾50%以上用量的土壤速效钾含量在块茎形成期后显著高于通氯化钾,同时,土壤水溶性钾与非特殊吸附钾含量较普通氯化钾分别增加8.4%~25.1%和9.3%~36.1%,土壤钾素供应充足。综上,包膜氯化钾50%以上用量有利于提高马铃薯产量、商品薯率和品质,同时增加土壤钾素供应。     

Effect of Root Zone Temperature on Accumulation of Molybdenum and Nitrogen Metabolism in Potato Plants

Abstract

Changes in root temperature caused by the application of plastic covers were studied in relation to the uptake and content of molybdenum (Mo) in the different organs of potato (Solanum tuberosum L. var. Spunta) plants (roots, tubers, stems, and leaves) and in relation to nitrogen (N) metabolism. For the semi‐forcing technique of mulching, four different covers were used: T ₁ (transparent polyethylene), T ₂ (white polyethylene), T ₃ (coextruded black and white polyethylene), and T ₄ (black polythylene). The control treatment had no mulch. The results revealed a positive and significant effect of plastic covers on root temperatures: T ₀ = 16°C, T ₁ = 20°C, T ₂ = 24°C, T ₃ = 27°C, T ₄ = 30°C. These thermal differences significantly influenced the Mo concentration, particularly in the T ₂ and T ₃ treatments in the leaves, roots, and tubers. The same temperatures significantly altered N metabolism in both the aerial and underground parts of the plants, and a strong interrelationship was found between Mo and nitrate reductase (NR) activity. The mulching of this crop proved to be a promising technique in phytoremediation.     

Changes in Metallic Cation Accumulation in Tomato (Solanum Lycopersicum L.) Affected by Boron and Potassium

The aim of this study was to determine the combined effects of boron (B) and potassium (K) on metallic cation accumulation, membrane permeability (MP) and growth of tomato (Solanum lycopersicum L.). Four levels of B (0, 5, 10 and 20 mg kg^?1) and two levels of K (0 and 200 mg kg^?1) were applied. B treatments, with or without K, enhanced the MP of leaves and the concentration of metallic cations except for manganese (Mn) and zinc (Zn) concentration as well B concentration. B uptake increased linearly with increasing B treatments. Metallic cation uptakes decreased at the highest B level. Excess B decreased the shoot and root biomass with and without K supply; however, K partially improved the detrimental effect of toxic B on plant growth. It was concluded that supplying K to growth media can be beneficial for alleviating plant growth reduction and imbalances of metallic cations accumulation caused by excess B.     

Effect of Nutritional Treatments on Physiological Characteristics and Tuberization of Potato Plants under Hydroponic Sand Culture

Under hydroponic condition, number and size of potato tubers are usually controlled by nutritional factors such as nitrogen (N), phosphorus (P), and pH. The main objective of the present study was to find an appropriate combination of N, P, and pH (with respect to tuber number) under hydroponic sand culture and to evaluate the physiological traits affected by nutrients and pH. A factorial experiment based on completely randomized design with four replications was conducted. Results showed that higher phosphorus concentration for 10 days increased tuber number per plant, but tuberization was not influenced by nitrogen interruption and intermittent reduction of pH. Neither N, P, nor pH treatments affected total nitrogen concentration of potato leaf, stem, and tuber. Higher phosphorus concentration increased the level of endogenous abscisic acid (ABA) and indole-3-acetic acid (IAA), induced tuberization and thereby increased net photosynthesis rate of potato plants.     

不同氮素形态配施氨基酸对翠冠梨生长及品质的影响研究

为探讨不同氮素配施氨基酸对梨树生长、产量及品质的影响,连续两年在8年生翠冠梨上开展了等氮条件下单施硝铵和尿素及配施氨基酸的研究。结果表明,单施硝铵处理较单施尿素处理显著增加了SPAD值、叶面积和百叶重,分别比尿素处理增加了2.8%、5.9%和7.2%。硝铵配施氨基酸处理显著增加了单果重。单施硝铵处理较单施尿素处理显著增加了单株产量、硬度和可溶性糖,有增加可溶性固形物和可滴定酸含量的趋势。花后110天硝铵配施氨基酸处理比单施硝铵处理显著增加了果糖、葡萄糖和蔗糖含量。各种糖在不同生育期所占比例不同,花后46天果实中山梨醇所占比例最高,约占总糖含量的69%~73%,葡萄糖和果糖比例相当;随着果实的发育,果糖和蔗糖比例逐渐增加,山梨醇比例降低。总体看来,硝铵配施氨基酸处理更有利于梨树生长和果实品质的提高。     

Effects of Iron and Zinc Fertilizers on the Accumulation of Fe And Zn Ions in Potato Tubers

The aim of this study was the evaluation of the methods to increase the yield of potato and the accumulation of iron (Fe) and zinc (Zn) in response to iron and zinc fertilizers. In these studies, ‘Marfona’ potato (Solanum tuberosum) cultivar was used in a statistical factorial design experiment with three factors: methods of fertilizer application (2 levels of spray and one level by irrigation), kind of fertilizers (iron and zinc) and levels of fertilizer, (0, 1, 2, and 3 mg L^?1). Results showed that iron and zinc fertilizers increased the tubers weight and the tubers number weight^?1 ratio (P < 0.01). Also, results showed that the effects of Fe and Zn fertilizers application in irrigation water was more significant (P < 0.01) than by spray application. In addition, application of Fe and Zn fertilizers resulted in higher concentrations of these ions in the harvested tubers improving their nutritional values.     

Effects of Seasonal Nitrate Flush on Nitrogen Metabolism and Soluble Fractions Accumulation in Two Rice Varieties

ABSTRACT

Two rice varieties, ‘Piaui’ (a landrace) and ‘IAC-47’ (an improved variety), were grown in nutrient solution containing 20 mg nitrate (NO₃ ^?)-nitrogen (N) L^{? 1} up to 32 days after germination (DAG). After this, a group of plants received 200 mg NO₃ ^?NL^{? 1}, while the other was kept at 20 mg NO₃ ^?NL^{? 1} up to 42 DAG. From 42 until 56 DAG, all plants received 5 mg NO₃ ^?NL^{? 1}. Plants were collected at 42 and 56 DAG, soluble fractions, nitrate reductase (NR) and GS enzymatic activities were determined. The nutritional history of the plants affected significantly the uptake and use of nitrogen (N), and should be taken into consideration in the studies of N-use efficiency. The variety ‘Piaui’ was more efficient than ‘IAC-47’ in N-uptake use, accumulating more NO₃ ^? in its tissues at the initial phases of its cycle for subsequent utilization.     

沼液对成都平原地区土壤氮、磷、钾含量及其平衡的影响

为探讨在成都平原地区冲积性水稻土上施用沼液后土壤氮磷钾元素含量的变化及养分平衡的状况,实现沼液资源化利用的绿色发展。采用田间试验,设置10个沼液施用量梯度,并以清水与常规化肥为对照。研究施用不同量沼液对土壤氮、磷、钾等养分含量的影响,并对田间各养分平衡状况进行综合分析。结果表明:当沼液施用量在108.0～126.0 t/hm~2时土壤全氮、碱解氮、全钾及速效钾含量较高,为1.35～1.42 g/kg、63.31～65.34 mg/kg、12.90～13.26 g/kg、45.45～59.25 mg/kg。随着沼液施用量的增加,土壤全磷含量(0.92～1.10 g/kg)变化不明显,而速效磷含量(8.49～18.85 mg/kg)较清水对照相比降低11.0%～59.9%。沼液处理组土壤氮、磷、钾元素的平衡范围为-25.61～66.68,-7.99～-15.34,-81.33～-145.82 kg/hm~2。当沼液施用量大于108.0 t/hm~2时,氮素能基本维持平衡。随沼液施用量增加,磷素亏损得到缓解,而钾素亏损加剧。施用适量沼液能提高土壤氮钾元素含量,但单施沼液难以满足土壤磷钾元素的平衡。因此,在实际生产过程中沼液施用量应控制在108.0～144.0 t/hm~2,并与10～15 kg/hm~2磷肥、115～120 kg/hm~2钾肥配合施用才能达到理想的效果。     

黑膜覆盖对旱地马铃薯块茎淀粉积累和关键酶活性的影响

为探明宁南半干旱雨养农业区马铃薯块茎中淀粉和关键酶活性对黑色地覆盖膜的响应机理,在2016年和2017年以宁南山区马铃薯主栽品种青薯9号为材料,通过两年大田试验,研究了黑膜覆盖(BF)、白膜覆盖(WF)和不覆膜(NF)对马铃薯块茎中淀粉及其组分积累、淀粉合成关键酶活性的影响。结果表明,与WF和NF相比,BF的直链淀粉、支链淀粉、总淀粉含量分别增加11.37%~35.68%、8.56%~27.05%、8.33%~27.60%,淀粉积累速率提高13.80%~37.90%;BF、WF、NF条件下块茎ADPG焦磷酸化酶(AGPase)、UDPG焦磷酸化酶(UGPase)、淀粉分支酶(SBE)、可溶性淀粉合酶(SSS)、束缚态淀粉合酶(GBSS)活性均呈单峰曲线变化,BF的淀粉合成关键酶活性明显高于WF和NF。与WF和NF相比,BF的AGPase活性分别增加12.81%和40.24%;UGPase活性分别增加15.34%和36.52%、SBE活性分别增加16.64%和44.17%、SSS活性分别增加13.69%和34.76%、GBSS活性分别增加15.75%和45.44%。BF产量较WF和NF分别提高14.54%和57.23%。相关分析表明,马铃薯块茎中直链淀粉、支链淀粉和总淀粉含量与AGPase、UGPase、SBE、SSS、GBSS活性在多数测定时期呈正相关(P<0.01)。因此,在干旱半干旱地区采用黑色地膜覆盖栽培能够通过影响淀粉合成关键酶,进而提高马铃薯块茎中的淀粉及其组分含量,最终影响马铃薯的产量。     

福建甘薯氮磷钾施肥指标研究

根据近年来在甘薯主产区完成的118个氮磷钾肥效田间试验结果,建立甘薯氮磷钾施肥指标体系,包括甘薯施肥效应和土壤肥力分级、土壤速效氮磷钾丰缺指标、不同产量水平的氮磷钾最佳用量和比例、土测值与最佳施肥量关系式以及施肥时期和施肥方法等5个方面内容。结果表明,土壤对甘薯产量的平均贡献率为62.5%,氮磷钾平均增产效果是N>K>P,均达显著水平;土壤碱解氮、Olsen-P和速效钾的高产临界指标分别为176 mg kg-1、17 mg kg-1和106 mg kg-1;平均经济施肥量是N165kg hm-2、P2O5 63 kg hm-2、K2O 204 kg hm-2,比例为1∶0.38∶1.24,但不同土壤肥力等级的推荐施肥量存在较大差异;土壤速效氮磷钾土测值与最佳施肥量之间满足指数模型,该式实现了根据土测值预测具体地块推荐施肥量的目的。研究结果为福建甘薯高效施肥提供了科学依据。     

超高产春玉米氮磷钾的吸收与分配

采用田间试验的方法,在供试玉米产量15 435 kg/hm2的超高产水平下,对春玉米氮、磷、钾吸收、分配与转移规律进行了系统研究。结果表明:在灌浆期前,超高产春玉米对氮、磷、钾养分吸收规律变化较为相似,即阶段吸收量和日均吸收量随生育时期的推进逐渐增大;而灌浆期后,植株对氮、磷、钾养分吸收趋势出现显著差异,即氮、磷吸收持续增高,钾吸收迅速下降。养分在各器官体内的分配特点体现在氮、磷分配随生长中心的转移而发生变化,而钾向生长中心的转移变化不明显。     

N、P、K肥对玉米幼苗吸收和积累重金属的影响

采用室内盆栽试验,研究复合污染土壤中施加氮肥（NH4Cl）、磷肥（Na2HPO4）和钾肥（KCl）对高生物量经济作物玉米（Zeamays L.）幼苗生长以及吸收和积累重金属的影响。结果表明,不同施肥方式和浓度处理对玉米生物量变化以及吸收重金属有不同影响,NH4Cl能显著提高玉米地上部生物量、土壤Pb、Cd有效态含量,增加玉米对重金属Pb、Cd、As的提取量,最大分别可提高1.7、2.0倍和1.2倍。不同施肥方式和处理浓度均显著影响土壤有效态Pb含量,Na2HPO4在中浓度处理时显著降低土壤Pb的有效性,高浓度时则显著增加土壤有效态As含量,使玉米地上部对As的积累量有明显提高。在不同的浓度水平下,钾肥处理使玉米提取Pb含量显著高于氮肥和磷肥,其中低浓度KCl处理使玉米提取Pb量比对照增加2.4倍。对Pb-Cd-As复合污染农田土壤来说,施用氮肥（NH4Cl）处理对强化玉米的修复效果最好。     

Interactive Effect of Sulfur and Nitrogen on Nitrogen Accumulation and Harvest in Oilseed Crops Differing in Nitrogen Assimilation Potential

ABSTRACT

Field experiments were conducted to determine the interactive effect of sulfur (S) and nitrogen (N) on nitrogen accumulation, its distribution in various plant parts, and nitrogen harvest of oilseed crops viz. rapeseed (Brassica campestris L. cv. ‘Pusa Gold’) and taramira (Eruca sativa Mill.) differing in their N-assimilation potential. Two combinations of S and N (in Kg/ha): 0S + 100N (?S+N) and 40S + 100N (+S+N) were used. The results showed that combined application of S and N (+S+N) significantly (P < 0.05) increased the nitrogen accumulation in both the genotypes at all the growth stages compared with N applied alone (?S+N). This increase in nitrogen accumulation was due to the improvement in the reduction of nitrate into reduced nitrogen as evident from higher nitrate reductase (NR) activity in the leaves of plants grown with both S and N, compared with N alone. Nitrate-N content in the leaves of plants grown with only N (?S+N) was higher compared to those grown with both S and N (+S+N), showing that combined application of S along with N (+S+N) appreciably reduced the nitrate content in the leaves due to higher NR activity. This decline in nitrate (NO₃ ^?) was followed by an overall increase in N-accumulation in the plants. Consequently, the nitrogen content in the plant was increased by 29–148% in rapeseed and 38-166% in taramira with +S+N treatment. Combined application of S along with N (+S+N) also increased seed protein content and nitrogen harvest index of both the genotypes. It is concluded that combined application of S along with N (+S+N) not only increased the N-accumulation, but also its mobilization towards economic sinks.     

施氮量对宁夏旱区马铃薯块茎淀粉形成和产量的影响

为探索不同施氮量对宁夏旱区马铃薯块茎淀粉形成和产量的影响机理,分别于2017年(平水年)和2019年(丰水年)在宁夏南部山区开展大田试验,以马铃薯栽培品种青薯9号为材料,采用单因素随机区组试验设计,以不施氮肥(N0)为对照,研究施纯氮量75(N1)、150(N2)、225 kg·hm-2(N3)对马铃薯淀粉含量、淀粉形...     

Effect of Potassium Fertilizers on Cotton Yield and Nitrogen Uptake Efficiency in an Aridisol

A field experiment was carried out to study the effect of different rates of potassium (K) fertilizer [0, 50, 100, and 150 potassium oxide (K₂O) ha^–1] in the presence of increased supply of nitrogen (N) (120, 180, and 240 kg N ha^–1) on cotton (Gossypium hirsutum L.) yield and the N and K use efficiencies using the ¹⁵N isotopic dilution technique. Potassium fertilizer increased cotton yield, which was significant and more pronounced with the application of N in the high level (N3). The greatest cotton yield (6442 kg ha^–1) was obtained in N2K3 treatment with an increase of 14% over the control. In addition, K fertilizer significantly increased N uptake efficiency in the N2 and N3 treatments. The greatest N uptake efficiency (98%) was in N2K3 treatment. The greatest K uptake efficiency (42%) was occurred in N3K1 treatment. In conclusion, the use of K fertilizer could be useful when growing cotton in soils of moderate to high N content to improve N uptake efficiency and consequently increase cotton yield.     

Influence of Nitrogen and Potassium Fertilization on Yield and Quality of Rosemary in Relation to Harvest Number

Rosemary is an important aromatic and spice plant. Its cultivation for production of its essential oil has gained importance in recent years. An experiment was conducted in a red sandy loam (Kandiustalf) soil to study the impact of cultivation of this multiharvest crop at different nitrogen (N) and potassium (K) application regimes on soil fertility and crop yield at the farm of the Central Institute of Medicinal and Aromatic Plants, Resource Center, Bangalore, during 2003–2005. The treatments consisted of all combinations of N levels (0, 150, and 300 kg per hectare per year) and K levels (0, 50, and 100 kg per hectare per year). Five harvests were taken at about 5‐month intervals. The results showed that initially the soil alone was able to meet the crop requirements for K, but with the progress of time, addition of K to the soil in the form of fertilizer became necessary for obtaining optimum yields. At the end of five harvests, exchangeable K in soil was significantly lower as application of K decreased from 100 to 50 and 0 kg per hectare per year. Soil fertility could be maintained and oil yields of rosemary from four harvests could be increased from 146.6 L to 344.7 L per hectare by application of 150 kg N and 100 kg K.