Prediction of grain protein content in winter wheat through leaf color measurements using a chlorophyll meter

A field trial was conducted over a 3-year period at the Hokkaido Kitami Agricultural Experiment Station to examine whether the grain protein content (GPC) of a winter wheat cultivar (Triticum aestivum L. cv. Chihokukomugi) suitable for Japanese noodle-making could be predicted before harvest. The prediction of the GPC was accurate based on the color of the second leaf (just below the flag leaf) at the end of the emergence of the inflorescence, when nitrogen application was graded. In order to evaluate the reliability of this test, a survey of 95 wheat fields in the eastern part of Hokkaido was also carried out during a 3-year period. The prediction of the GPC for this cultivar based on the color of the second leaf was less accurate across many sites. The results of this survey, however, suggested that the leaf color could be used as an index for ranking the GPC as low or high in relation to processing requirements. When the leaf color value of the second leaf measured with a chlorophyll meter at the end of the emergence of the inflorescence was less than 40, it was predicted that the GPC would be lower than the processing requirement. This index could be applied to the cultivars grown in the eastern part of Hokkaido, except for those grown on peat soils.     

Possibility of using a SPAD chlorophyll meter to establish a normalized threshold index of nitrogen status in different potato cultivars

Leaf SPAD values are thought to be an indicator of potato nitrogen (N) status; however, when making plant N diagnosis, no single threshold leaf SPAD value can be used for all potato cultivars because of cultivar variation. We conducted field experiments over 3?years to test whether the difference in SPAD values between the upper and lower leaves was consistent among potato cultivars, and whether this value responded to N application levels. The results showed cultivar affects the SPAD values of the fourth and eighth leaves (SPAD_L4 and SPAD_L8), but not SPAD_L4-8, the difference between SPAD_L4 and SPAD_L8. Moreover, the SPAD_L4-8 values responded well to N application levels. Therefore the SPAD_L4-8 could be applied as a general index of N status across different potato cultivars, and establishing a threshold SPAD_L4-8 value might be more useful in guiding N fertilization recommendations for different potato cultivars.     

小麦叶片SPAD空间分布及其与氮素营养状况的关系

利用2个小麦品种在3个施氮水平下的田间试验数据,研究了小麦叶片氮素状况和SPAD（Soil and Plant Analyzer Development）值随叶位的空间分布特征,并对不同叶位叶片的氮素状况、SPAD值之间及其与叶片平均氮素状况之间的相关性进行分析。结果表明,随施氮量的增加,顶1叶、顶2叶、顶3叶、顶4叶的氮含量和氮积累量都呈增加趋势,并且基本呈顶1〉顶2〉顶3〉顶4的顺序;各叶位SPAD值呈两段式变化规律,开花前顶2叶SPAD值最大,开花后呈顶1〉顶2〉顶3〉顶4的趋势。顶3叶与顶2叶的SPAD值分别与叶片平均氮含量和氮积累量的相关关系最密切。     

Relationship between Nitrogen Use Efficiency and Response Index in Winter Wheat

Although nitrogen use efficiency (NUE) of small grains is well documented at 33% worldwide, there has been little research relating NUE to yield factors. This study examined the relationship between NUE and the response index at harvest (RI_HARVEST) of winter wheat (Triticum aestivum L.). Yield data from a long-term fertility study established at Lahoma, Oklahoma in 1971 was used to explore the relationship. In this report, six nitrogen (N) rates at non-limiting levels of P and K were evaluated. Regression analysis showed a positive relationship between NUE and RI_HARVEST for all years across all N rates (r² = 0.37). But this relationship was improved (r² = 0.45) when both RI_NDVI and RI_HARVEST were included in the model. The linear relationship between NUE and RI_HARVEST was significantly improved, when yield data and corresponding NUE were separated according to the annually applied fixed N rate. As the N rate increased the resulting slope of the relationship between NUE and RI was reduced. These analyses also demonstrate that temporal variability in NUE exists and that NUE can be predicted.     

小麦氮素营养与籽粒灌浆期氮素转移的研究进展

对小麦氮素营养与籽粒灌浆期氮素转移研究进展进行了综述。过去进行的大量研究结果表明,小麦籽粒最终累积的氮素有相当一部分来自于灌浆期间营养器官中氮素的再转移,来自营养器官氮(内源氮)与土壤中新吸收氮(外源氮)的比例基本上是1:2。因此,花后营养器官氮素营养水平是决定小麦籽粒产量、籽粒中氮素累积量和蛋白质含量的一个重要因素。灌浆期间营养器官氮素向籽粒发生转移的同时,常常伴随着叶片光合性能的下降和叶片的衰老。不同基因型品种在灌浆期的氮素转移程度不同,表现为随品种演替,旗叶、茎秆和叶鞘中氮素的输出率增加,而转移氮对籽粒氮的贡献率却下降。氮收获指数(NHI)可以描述植物向籽粒分配氮的能力,是衡量作物对氮利用效率的指标。氮收获指数存在显著的基因型差异,虽然现代小麦品种吸氮量高于古老品种,但氮收获指数在现代和古老小麦品种间的差异因不同研究者而异,有的认为现代品种高于古老品种,但也有人认为与年代无关;小麦氮收获指数一般在0.55～0.80之间,很少超过0.8。过去对小麦籽粒灌浆期间的氮素转移虽然进行了大量研究工作,取得了许多重要进展,但仍有许多问题需要进一步深入研究,如根冠关系和灌浆过程中氮素转移的相互关系,田间个体和群体调控及不同高产栽培模式下灌浆过程中氮素转移的     

镉污染水稻土中水稻氮素营养的SPAD诊断

治理和安全利用重金属污染水稻土过程中,氮素营养的快速诊断是制定合理施肥措施的基础。叶绿素测定仪SPAD已广泛应用于非污染耕地土壤中玉米、小麦和棉花等作物的氮素营养诊断,然而针对镉污染条件下的不同类型水稻土,SPAD在诊断水稻氮素营养的适应性方面仍然缺乏系统研究。本研究采集了我国水稻主产区的21种典型水稻土,以德农2000为供试水稻品种,设置无镉污染、轻度镉污染、重度镉污染处理的水稻盆栽试验,研究水稻叶片SPAD值对镉污染水稻土氮素供应诊断和水稻产量预测的能力。结果表明:水稻土类型影响了水稻叶片SPAD值对不同水平镉污染的响应,土壤p H是主控因子,其相对影响的平均值为20%。水稻叶片SPAD值与不同生育期水稻籽粒氮素含量显著正相关,其中拔节期的相关系数最大;同时水稻叶片SPAD值与水稻营养生长期(苗期至拔节期)土壤溶液总氮和铵态氮含量呈显著正相关,且不受镉污染程度和土壤类型的影响。总体上,不同生育期(尤其是拔节期)水稻叶片的SPAD值可以表征镉污染条件下不同类型水稻土氮素养分供应对水稻氮素营养的影响。     

Use of Chlorophyll Meter to Assess the Effect of Nitrogen on Sweet Pepper Development and Growth

Abstract

The evolution of both leaf expansion and chlorophyll content was assessed in potted sweet pepper plants subjected to four different levels of nitrogen (mg N/kg of soil): N1 = 25 (basal dressing); N2 = 50 (basal dressing); N3 = 100 (basal dressing and one side dressing); and, N4 = 150 (basal dressing and two side dressings). In each plant, the first leaves (numbered 1–5) were chosen at the main stem and the next four ramifications. The relative chlorophyll content of leaves 1 to 5, from all treatments, was obtained by a portable chlorophyll meter, SPAD-502, twice a week. The SPAD readings were subsequently converted into total chlorophyll (μ g cm^{? 2}). The plant dry weight, the number of fruits per plant, and the N content of leaves were measured at final harvest (70 d after transplantation, DAT). Until the first side dressing (35 DAT), the increase in chlorophyll content was similar in all treatments, decreasing afterward under the N1 and N2 treatments (leaves 1 and 2), while under the N3 and N4 treatments the increase in the chlorophyll content continued after the first side dressing. The application of the second side dressing (53 DAT) under the N4 treatment induced a subsequent increase in chlorophyll content in all leaves compared with those of N3. An early senescence was observed under the N1 and N2 treatments compared with the others. Applied N in side dressing led to an increase in leaf width (leaves 2–5) and longevity, mainly in leaves 2 and 3, and a subsequent increase under fruit number and fruit dry weight under the N3 and N4 treatments.     

不同施肥下冬小麦生长过程中土壤矿质氮变化及其与冬小麦叶片SPAD值的关系

通过调查分析,研究了土长期施肥条件下土壤中矿质氮含量变化及其与地上冬小麦叶片SPAD值。结果表明:(1)整个冬小麦生长期不同土层硝态氮和铵态氮的变化趋势不一致,硝态氮含量是先下降后上升的变化,而铵态氮含量呈一直上升的变化趋势。在没有施过氮肥的处理中,0—20cm,20—40cm土层中土壤硝态氮、铵态氮含量显著低于施用氮肥的处理;(2)冬小麦生长时期,各个处理叶片SPAD值各异,但都是先升高后下降的变化,无氮肥施用的叶片SPAD值低于施氮肥的处理;(3)冬小麦各个生长时期叶片SPAD值与土壤不同层次(0—20cm,20—40cm)硝态氮含量呈正相关关系,而与铵态氮含量相关性不显著,这表明小麦是对硝态氮较为敏感的作物。本试验结果可以为进一步合理调控氮肥施用、明确施氮对小麦产量和品质的影响提供一定的基础依据。     

施氮水平对强筋小麦氮素同化及籽粒蛋白质组分积累的影响

在高产条件下研究了施氮水平对强筋小麦济麦20氮素同化及籽粒蛋白质组分积累和品质的影响,结果表明,在0~195 kg/hm2施氮量范围内,增施氮肥显著提高旗叶硝酸还原酶和谷氨酰胺合成酶活性,提高各器官氮素含量和积累量,促进籽粒单体蛋白、可溶性和不溶性谷蛋白积累,提高籽粒蛋白质含量及可溶性和不溶性谷蛋白占总蛋白的比例,改善籽粒品质;285 kg/hm2施氮量处理与195 kg/hm2施氮量处理相比,旗叶硝酸还原酶和谷氨酰胺合成酶活性及籽粒蛋白质含量均无显著变化,但单体蛋白含量及占总蛋白质的比例升高,可溶性和不溶性谷蛋白含量及占总蛋白质的比例降低,籽粒品质下降,兼顾高产和优质的适宜施氮量为105~195 kg/hm2。     

Optimizing the Nitrogen Management Strategy for Winter Wheat in the North China Plain Using Rapid Soil and Plant Nitrogen Measurements

Excessive nitrogen (N) fertilizer with improper split-application in small-scale farming is widespread for reducing N use efficiency and polluting the environment. The objective of this study was to develop a strategy for providing winter wheat with twice-topdressing N by quickly measuring the soil and plant N status. During the period 2009–2011, a field experiment was conducted for winter wheat cultivar Zhongmai-175 in the North China Plain. The mineral N (Nmin) pool at a soil depth of 0–90 cm and topdressing N twice, as total N supply, was gradually increased from 0 to 420 kg N ha^–1 to mimic the farmers´ practices. Measurements with the Soil Plant Analysis Development (SPAD) meter were taken on the uppermost fully expanded leaf, and the SPAD index was expressed relative to SPAD readings of sufficiently fertilized plants. Grain yield exhibited linear-plus-plateau responses to total N supply with a significant difference between years, the r² ranged from 0.73 to 0.94. With a basal N application of 30 kg ha^–1, the soil Nmin at 0–90 cm supplemented by twice-topdressing N (1:1 ratio) at Zadoks growth stage (ZGS) 22–23 in early spring and ZGS 47–52 was required at 150–165 kg N ha^–1 to achieve a maximum grain yield of 3.9–5.3 t ha^–1. The SPAD index exhibited a strong exponential response to N supply irrespective of plant growth stage and year (r² = 0.95–0.97); the value of 0.94 was critical in denoting N deficiency from sufficiency status. The N topdressing at ZGS 47–52 could be precisely modified/estimated by the equation y = 161.7–218x^5.16, where x is the SPAD index. Since SPAD readings varied significantly from year to year, our study suggests that it might be difficult to precisely manage field N for winter wheat.     

Effect of Nitrogen and Trinexapac-Ethyl Rates on the SPAD Index of Wheat Leaves

ABSTRACT

The objective of this study was to evaluate the effect of nitrogen (N) and trinexapac-ethyl (TE) rates on the SPAD index in wheat flag leaf. The treatments were five N rates (30, 60, 90, 120, 150 kg ha^?1) combined with four TE rates (0, 63, 125, 188 g ha^?1). The experiment was carried out in a randomized block design with four repetitions. SPAD index, leaf N content and grain yield showed quadratic response to the increase in N rates, whereas area, wet and dry weight of flag leaf presented linear increase. TE caused linear increase in SPAD index, linear decrease in leaf area, reduction in grain yield with smaller N rates and increase with larger N rates. The N content, and the wet and dry weight of flag leaf were not affected by TE. SPAD calibration to estimate N status in wheat should be specific for each TE rate.     

15Nitrogen Study on Accumulation and Allocation of Nitrogen Applied at Anthesis on Top of Early Nitrogen Applications in Winter Wheat

The objective of this experiment was to assess the effect of interaction between early and late nitrogen (N) fertilization on grain yield, protein content, and distribution of N between organs of winter wheat. Wheat was grown in pots in vegetation house. Experiment has been carried on in three years 2001–2003, each year in four replications. Wheat supplied with 0.9, 1.4, 1.9, 2.4, and 2.9 g N/pot was top dressed at anthesis by 0.85 g labeled nitrogen ¹⁵N/pot. Plants supplied with 0.9 g N/pot enhanced grain yield by 28% and gluten content by 62% and plant supplied with 2.9 g N/pot by 27% and 13%, respectively. The amount of ¹⁵N fertilizer stored in plant organs decreased proportionally to the amount of previously accumulated nitrogen, but the share of ¹⁵N in plant parts was almost constant. Grain accumulated about 86% of total ¹⁵N taken up by plants. Irrespective on nitrogen status prior to anthesis, about 50% of ¹⁵N has been used for gluten formation. The percentage of ¹⁵N recovery fraction ranged from 79% to 86%.     

Use of a hand‐held chlorophyll meter in winter wheat: Evaluation of different measuring positions on the leaves

A portable chlorophyll meter (Minolta SPAD‐502) was used to assess the nitrogen status of winter wheat (Triticum aestivum L.) in two fertilizer trials at Apelsvoll Research Centre, located in south‐east Norway. The midpoint of the last fully developed leaf was found to be the best position on the winter wheat plant on which to take chlorophyll meter readings. This conclusion was reached after examination of the relationships between soil‐plant analyses development (SPAD) readings taken at different positions on the plant and leaf nitrogen concentration, grain yield and grain protein content. Emphasis was also laid on finding a measuring position that was convenient from a practical point of view. The relationships between chlorophyll meter readings and the parameters investigated were better at Zadoks growth stage (GS) 49 than earlier in the season at GS 31.     

Contribution of Nitrogen Fixed by Mung Bean to the Following Wheat Crop

Crop rotation is a supportive management practice in which legumes greatly improve the growth and yield of subsequent cereal crops, particularly wheat. In this study, effects of mung bean (Vigna radiata)?? on the yield and quality of grains of following wheat crop were determined. An experiment was carried out with two sets of wheat (Triticum aestivum) crops: one grown following mung bean (Vigna radiata) crop and the other grown after a fallow period. Concentrations of macronutrients, sugar, protein, amino acids, and phytohormones of wheat were determined. The grain protein concentration of wheat was improved if cultivated after mung bean, and nitrogen and other macronutrients of wheat were increased significantly. In case of phytohormone contents in the wheat crop, abscisic acid concentration showed no change, but the concentrations of gibberellin increased significantly by 41% and indole acetic acid by 30% as a result of crop rotation. Sugar content in wheat cultivated after mung bean BRM-318 showed 10% increase and wheat protein content increased by 17% and 20%, respectively. The present study demonstrated that crop rotation simulated yield and improved the nutritional value of wheat grain compared to wheat which followed the fallow period.     

Aluminum toxicity in maize: Biomass production and nutrient uptake and translocation

The effect of increasing aluminum (Al) concentrations on root nutrient contents along with the concurrent translocation to the shoot of C₄ plants prompted this study. Two‐week‐old maize (Zea mays cv XL‐72.3) plants were therefore submitted for 20 days to Al concentrations ranging from 0 to 3.00 mM in a medium with low ionic strength were used as a test system. Aluminum concentrations in root tissues showed a 3‐fold increase between 0 and 3.00 mM Al treatment, and was not detected in the shoot. Root plasma membrane‐H⁺ ATPase activity decreased after the 0.33 mg L^‐1 Al treatment, while membrane permeability increased up to 1.00 mM Al treatment. Root and shoot biomass decreased after the 0.33 mM Al treatment. All elements in the roots, except potassium (K), manganese (Mn), and zinc (Zn) were highest for plants treated with 0.33 mM Al. Potassium increased continuously between 0 and 3.00 mM Al treatments, and iron (Fe) decreased above 0.33 mM. Only a slight decrease in nitrogen (N) was observed. All the measured nutrients in shoots, except N, Mn, and Fe decreased above 0.33 mM, but calcium (Ca) and magnesium (Mg) had little variation as Al varied. Data indicated that maximum net uptake for mineral nutrients, except Mn, occurred up to 0.33 mM Al. Translocation of phosphorus (P), K, Mn, and Zn decreased above 0.33 mM Al, N, and Ca decreased when any Al was added, and no clear trend was observed for Mg and Fe. Between the 0 and the 3.00 mM Al treatments, electrolytic conductance did not increased significantly indicating that the observed inhibitions of translocation from roots to shoots were not directly related to increasing membrane degradation.     

Effect of Nitrogen Topdressing at Anthesis and the Association of Flag-Leaf Nitrogen with Grain Protein Concentration in Irrigated Spring Wheat

ABSTRACT

Grain protein is an important component affecting the market value of hard red spring wheat (Triticum aestivum). In high-yielding irrigated environments, consistently attaining desired protein levels is a chronic problem. Nitrogen (N) management has a strong effect on protein concentration. The objective of this experiment was to evaluate the robustness of using flag-leaf N concentration as a tool for guiding in-season N application in order to obtain high-protein wheat. Three on-farm trials were conducted (each location using a cultivar of the farmer's choice) where N rates were varied. Nitrogen rates evaluated were 0, 79, 157, 236, and 314 kg ha^?1. To evaluate the benefit of topdressing, all N was applied basally, or 45 kg ha^?1 N was reserved from the basal dose for application at heading. Yield and protein response to applied N were variable across the three sites. This study postulated that this response was a function of initial soil-N availability. Where there was a yield response to N, it appeared that reserving a portion of N for topdressing increased protein but tended to decrease yield. At levels of N where yield was not limited, reserving a portion of N for topdressing did not appear to affect yield or protein. Although a linear relationship between grain protein and flag-leaf N was obtained by pooling data across sites, there was enough variation in this relationship to limit its utility as a tool for guiding in-season N application.     

Estimation of Optimum Nitrogen Fertilizer Rates in Winter Wheat in Humid Mediterranean Conditions,II: Economically Optimal Dose of Nitrogen

Cereal grain and nitrogen (N) fertilizer prices have varied greatly in recent years. The aim of this study was to determine the optimum dose of N fertilizer needed to maximize revenues of soft red winter wheat in Alava (northern Spain). Economically optimum rates of N application (N_yield) ranged from 142 to 174 kg N ha^?1 depending on the price of both N fertilizer and wheat. Growers received an extra income of 0.006 [euro] kg^?1 if the grain protein content was greater than 12.5%, with the minimum required N dose to obtain this value (N_prot) being 176 kg ha^?1. The extra amount of N fertilizer required over N_yield to reach N_prot ranged from 2 to 34 kg N ha^?1, and the extra benefits associated varied from 24 to 36 [euro] ha^?1.     

机器学习结合高光谱植被指数与SPAD值估算冬小麦氮含量

冬小麦叶片氮含量与叶片光合作用和营养状况密切相关,直接影响植株生长发育,而茎秆中的氮含量与茎秆中纤维素、半纤维素和木质素的比例和含量密切相关,直接影响茎秆质量及植株的抗倒伏能力。然而,有关对冬小麦茎秆氮含量估算研究较为有限,限制了从氮含量角度判断茎秆质量及对倒伏的预测能力。为精准估算冬小麦不同器官（叶片、茎秆）氮含量,该研究通过2年田间试验,获取冬小麦4个关键生育期（拔节期、抽穗期、开花期、灌浆期）和3种施氮水平条件下（N1、N2和N3）的冠层光谱反射率、叶片、茎秆氮含量及叶片SPAD (soil and plant analyzer development, SPAD)值。分析了不同生育期和施氮水平条件下高光谱植被指数对叶片和茎秆氮含量的敏感性,并结合5种常用的机器学习算法：随机森林回归（random forest regression,RFR）、支持向量回归（support vector regression,SVR）、偏最小二乘回归（partial least squares regression,PLSR）、高斯过程回归（gaussian process regression,GPR）、深度神经网络回归（deep neural networks,DNN）构建冬小麦叶片和茎秆氮含量估算模型。结果表明：高光谱植被指数对叶片和茎秆氮含量的敏感性受到生育期和施氮水平的影响。在灌浆期,最佳植被指数双峰冠层植被指数 DCNI（double-peak canopy nitrogen index）对叶片氮含量的敏感性最高,R²为0.866。对茎秆氮含量,在抽穗期的敏感性最高,最佳植被指数归一化叶绿素比值指数 NPQI（normalized phaeophytinization index）与氮含量相关系数R²＝0.677。施氮水平的提升增加了光谱植被指数对茎秆氮含量的敏感性。结合SPAD值的机器学习算法提升了氮含量的估算精度,对叶片氮含量,在不同生育期和施氮水平条件下估算精度提升了1%～7%,其中在全生育期的归一化均方根误差NRMSE从0.254提升到0.214,抽穗期的NRMSE提升最大,从0.201提升到0.128。对茎秆氮含量,全生育期的NRMSE从0.443提升到0.400,抽穗期的NRMSE提升最大,从0.323提升到0.268。在全生育期,结合SPAD值的DNN模型对叶片（R²=0.782、NRMSE=0.214）和茎秆（R²=0.802、NRMSE=0.400）氮含量的估算精度最佳。研究说明,SPAD值与光谱植被指数结合有利于提升冬小麦不同生育期和施氮水平条件下叶片和茎秆氮含量的估算精度。     

追氮量对黑小麦品种(系)籽粒产量和品质的影响

为探究追氮量对不同黑小麦品种(系)籽粒产量和品质的影响,本研究采用大田裂区试验设计,主区为11个黑小麦品种(系),副区为追施氮量0(N0,对照)、34.5(N1)、51.8(N2)、69.0(N3)kg·hm²,于小麦灌浆期测定旗叶叶绿素含量(SPAD值),成熟期测定籽粒产量及产量构成要素、籽粒氮素含量和品质指标。结果表明,旗叶SPAD值在追氮处理下均增加,除B145-2、L10056、L10058的旗叶SPAD值在N1最高,其余8个品种(系)的旗叶SPAD值均随追氮量增加而升高。追施氮肥均有利于增加黑小麦产量,与N0相比,N1、N2和N3的产量增幅分别为15.2%~99.7%、17.5%~80.9%和0.7%~69.9%。11个品种(系)获得最高产量的适宜追氮量因品种而异,其中L10056、B120-2、运黑14207籽粒产量随追氮量增加而增加,B145-2、L10058籽粒产量在N2最高,其余6个品种(系)籽粒产量在N1最高。追氮量、品种及二者交互均对产量及构成因素存在极显著影响。追施氮肥能够提高多数黑小麦品种籽粒氮素含量,其中B145-2、L10058和运黑14207在N2最高,18鉴46、临黑131、冬黑1号、临黑187、2002-4、运黑161在N1最高。追氮量与籽粒产量、氮含量、蛋白含量呈正相关(相关性不显著),而产量与籽粒蛋白含量及多数品质指标存在极显著负相关关系。综合考虑黑小麦产量及品质的提高,追氮量在34.5~51.8 kg·hm²之间较为适宜。本研究结果可为黑小麦合理施用氮肥提供参考。     

追氮量对黑小麦品种(系)籽粒产量和品质的影响

为探究追氮量对不同黑小麦品种(系)籽粒产量和品质的影响,本研究采用大田裂区试验设计,主区为11个黑小麦品种(系),副区为追施氮量0(N0,对照)、34.5(N1)、51.8(N2)、69.0(N3)kg·hm²,于小麦灌浆期测定旗叶叶绿素含量(SPAD值),成熟期测定籽粒产量及产量构成要素、籽粒氮素含量和品质指标。结果表明,旗叶SPAD值在追氮处理下均增加,除B145-2、L10056、L10058的旗叶SPAD值在N1最高,其余8个品种(系)的旗叶SPAD值均随追氮量增加而升高。追施氮肥均有利于增加黑小麦产量,与N0相比,N1、N2和N3的产量增幅分别为15.2%~99.7%、17.5%~80.9%和0.7%~69.9%。11个品种(系)获得最高产量的适宜追氮量因品种而异,其中L10056、B120-2、运黑14207籽粒产量随追氮量增加而增加,B145-2、L10058籽粒产量在N2最高,其余6个品种(系)籽粒产量在N1最高。追氮量、品种及二者交互均对产量及构成因素存在极显著影响。追施氮肥能够提高多数黑小麦品种籽粒氮素含量,其中B145-2、L10058和运黑14207在N2最高,18鉴46、临黑131、冬黑1号、临黑187、2002-4、运黑161在N1最高。追氮量与籽粒产量、氮含量、蛋白含量呈正相关(相关性不显著),而产量与籽粒蛋白含量及多数品质指标存在极显著负相关关系。综合考虑黑小麦产量及品质的提高,追氮量在34.5~51.8 kg·hm²之间较为适宜。本研究结果可为黑小麦合理施用氮肥提供参考。