Effect of Nitrogen and Potassium Stress and Cultivar Differences on Potassium Ions and Nitrate Uptake in Sugarcane

ABSTRACT

This study was conducted to evaluate the effect of nitrogen (N) and potassium (K) stress on nitrate (NO₃ ^?) and potassium ion (K⁺) uptake of two sugarcane (Saccharum officinarum L.) cultivars known to differ with regard to their resistance to drought and salinity stress. The plants were hydroponically grown in a greenhouse and subjected to varying levels of N and K. Nitrogen and K stress increased root affinity for NO₃ ^? and K⁺ absorption by increasing apparent maximum uptake rate (V_max) and decreasing apparent affinity (K_m) for NO₃ ^? and K⁺ uptake in both cultivars. In addition, dry-matter allocation to roots increased with decreasing N and K supply. The results suggest that the acclimation to long-term N starvation in sugarcane involves regulation of both nitrate-specific assimilatory steps and growth responses. The cultivar ‘H69-8235,’ which was drought and salinity resistant, showed greater adaptability to a low- nutrient environment due to its higher root allocation and affinity for NO₃ ^? and K⁺ under N and K stress, suggesting that the resistance of sugarcane to multiple stresses may involve a general stress-response system.     

Nitrate/ammonium ratio effects on nutrient solution pH,dry matter yield,and nitrogen uptake of sorghum 1

Abstract

Sorghum [Sorghum bicolor (L.) Moench] seedlings were grown in nutrient solutions in a growth chamber to investigate the effects of different ratios of NO₃ ^– and NH₄ ⁺ on nutrient solution pH, dry matter yield, and N uptake. Nutrient solutions and plant tissues were assayed throughout the time plants grew in the nutrient solutions.

Nutrient solution pH depended on source of N. The pH rose to near 8 with NO₃ ^– as the sole source of N and decreased to near or below 4 with NH₄ ⁺ added to the solutions. Upon depletion of NH₄ ⁺ from solution, pH values rose abruptly to near 8 and remained near this value throughout the duration of the experiments. Dry matter yield was generally higher for plants grown with some NH₄ ⁺ compared to plants grown with NO₃ ^– alone. Nitrogen uptake was generally higher in plants grown with the higher proportions of NH₄ ⁺. Nitrogen concentrations remained unchanged with plant age as NO₃ ^–/ NH₄ ⁺ ratio varied. For solutions low in NH₄ ⁺, N concentrations in roots increased with plant age. Severe Fe deficiency appeared in plants when solution pH reached and remained above 7.     

Effects of Seasonal Nitrate Flush on Nitrogen Metabolism and Soluble Fractions Accumulation in Two Rice Varieties

ABSTRACT

Two rice varieties, ‘Piaui’ (a landrace) and ‘IAC-47’ (an improved variety), were grown in nutrient solution containing 20 mg nitrate (NO₃ ^?)-nitrogen (N) L^{? 1} up to 32 days after germination (DAG). After this, a group of plants received 200 mg NO₃ ^?NL^{? 1}, while the other was kept at 20 mg NO₃ ^?NL^{? 1} up to 42 DAG. From 42 until 56 DAG, all plants received 5 mg NO₃ ^?NL^{? 1}. Plants were collected at 42 and 56 DAG, soluble fractions, nitrate reductase (NR) and GS enzymatic activities were determined. The nutritional history of the plants affected significantly the uptake and use of nitrogen (N), and should be taken into consideration in the studies of N-use efficiency. The variety ‘Piaui’ was more efficient than ‘IAC-47’ in N-uptake use, accumulating more NO₃ ^? in its tissues at the initial phases of its cycle for subsequent utilization.     

Nitrogen fertilization inhibits soil microbial respiration regardless of the form of nitrogen applied

We tested how amendments of different forms of nitrogen (N) affect microbial respiration rates by adding six different forms of N (NH₄NO₃, (NH₂)₂CO (urea), KNO₃, NH₄Cl, (NH₄)₂SO₄, Ca(NO₃)₂) to three distinct soils. All inorganic N forms led to a net reduction in microbial respiration, and the magnitude of the observed response (up to 60 % reduction) was consistent across all soils and negatively correlated with N concentration. Urea also reduced respiration rates in nearly all cases, but the effect was attenuated by the associated input of labile organic carbon. We observed decreases in respiration regardless of soil type, the specific N counter ion, N added as NH₄⁺ or NO₃⁻, or the effects of N form on soil pH, suggesting that decreases in respiration rates were mainly a direct result of the increase in soil N availability, rather than indirect effects caused by the form of N added.     

Interactive Effects of Nitrogen Source and Salinity on Growth Indices and Ion Content of Indian Mustard

ABSTRACT

The interactions between salinity and different nitrogen (N) sources nitrate (NO₃ ^?), ammonium (NH₄ ⁺), and NO₃ ^? + NH₄ ⁺ were investigated on Indian mustard (Brassica juncea cv. RH30). Treatments were added to observe the combined effect of two salinity levels (8 and 12 ds m^{? 1}) and three nitrogen sources (NO₃ ^?, NH₄ ⁺, and NO₃ ^? + NH₄ ⁺) on different growth parameters and mineral composition in different plant parts, i.e., leaves, stem, and root. Salinity has been known to affect the uptake and assimilation of various essential nutrients required for normal growth and development. Different growth parameters, i.e., leaf area, dry weight of different plant parts, absolute growth rate (AGR), relative growth rate (RGR), and net assimilation rate (NAR) declined markedly by salinity at pre-flowering and flowering stages. All growth indices were less sensitive to salinity (12 d s m^{? 1}) with the nitrate form of nitrogen. It is pertinent mention that a high dose (120 kg ha^{? 1}) of nitrogen in ammonium form NH₄ ⁺, acted synergistically with salinity in inhibiting growth. Plants fed with combined nitrogen (NO₃ ^? + NH₄ ⁺) had an edge over individual forms in ameliorating the adverse effects of salinity on growth and yield. Under salt stress, different nutrient elements such as N, phosphorus (P), potassium (K⁺), and magnesium (Mg^{2 +}) were decreased in different plant parts (leaves, stem, and root). The maximum and minimum reduction was observed with ammoniacal and combined form of nitrogen, respectively, while the reverse was true of calcium (Ca^{2 +}), sodium (Na⁺), chloride (Cl^?), and sulfate (SO₄ ^2?) at harvest. Nitrogen application (120 Kg ha^{? 1}) in combined form had been found to maintain highest concentrations of N, P, Mg^{2 +}, and Ca^{2 +} along with reduced concentrations of Na⁺, Cl^?, and SO₄ ^{2 ?}. However, reverse was true with ammoniacal form of nitrogen.     

Accumulation and partitioning of biomass and soluble carbohydrates in maize seedlings as affected by source of nitrogen,nitrogen concentration,and cultivar 1

Abstract

Seedlings of four maize hybrids were grown hydroponically to investigate the impact of different N sources (Ca(NO₃)₂, (NH₄)₂SO₄ and a 1:1 mixture of both) on (i) production and partitioning of root and shoot dry matter, (ii) concentration of soluble carbohydrates in roots and shoots and their partitioning to these plant parts, (iii) concentration of starch in the shoot, and (iv) N uptake. During the main phase of the experiments (duration 14d), the plants were grown in a greenhouse at 25/22°C day/night temperatures and a photoperiod of 16h. Nitrogen was supplied at three concentrations (2.8, 28, and 280 ppm). The root‐zone pH was 6.5. Under the lowest N supply, the N sources produced similar root and shoot dry matters. At the highest N level (280 ppm), NO₃‐fed plants were superior. In contrast, the mixture of NH₄ and NO₃ ^? was optimum at 28 ppm. More or less pronounced N form by N concentration interactions were also found in the concentration and distribution of soluble carbohydrates and in all remaing traits. There were almost statistically significant cultivar by N form interactions in shoot dry matter (P = 0.07) and total dry matter (P = 0.06), indicating the existence of considerable genotypic variation in sensivity to NH₄‐N.     

Comparative Effect of Nitrogen Forms on Nitrogen Uptake and Cotton Growth Under Salinity Stress

The effects of nitrogen (N) forms (ammonium- or nitrate-N) on plant growth under salinity stress [150 mmol sodium chloride (NaCl)] were studied in hydroponically cultured cotton. Net fluxes of sodium (Na⁺), ammonium (NH₄⁺), and nitrate (NO₃^?) were also determined using the Non-Invasive Micro-Test Technology. Plant growth was impaired under salinity stress, but nitrate-fed plants were less sensitive to salinity than ammonium-fed plants due mainly to superior root growth by the nitrate-fed plants. The root length, root surface area, root volume, and root viability of seedlings treated with NO₃-N were greater than those treated with NH₄-N with or without salinity stress. Under salinity stress, the Na⁺ content of seedlings treated with NO₃-N was lower than that in seedlings treated with NH₄-N owing to higher root Na⁺ efflux. A lower net NO₃^? efflux was observed in roots of nitrate-fed plants relative to the net NH₄⁺ efflux from roots of ammonium-fed plants. This resulted in much more nitrogen accumulation in different tissues, especially in leaves, thereby enhancing photosynthesis in nitrate-fed plants under salinity stress. Nitrate-N is superior to ammonium-N based on nitrogen uptake and cotton growth under salinity stress.     

Growth,Nutrition, and Photosynthetic Response of Black Walnut to Varying Nitrogen Sources and Rates

ABSTRACT

Black walnut (Juglans nigra L.) half-sib 1+0 seedlings were exponentially fertilized with ammonium (NH₄ ⁺) as ammonium sulfate [(NH₄)₂SO₄], nitrate (NO₃ ^?) as sodium nitrate (NaNO₃), or a mixed nitrogen (N) source as ammonium nitrate (NH₄NO₃) at the rate of 0, 800, or 1600 mg N plant^?1 and grown for three months. One month following the final fertilization, N concentration, growth, and photosynthetic characteristics were assessed. Compared with unfertilized seedlings, N addition increased plant component N content, chlorophyll content, and photosynthetic gas exchange. Net photosynthesis ranged from 2.45 to 4.84 μmol m^?2 s^?1 for lower leaves but varied from 5.95 to 9.06 μmol m^?2 s^?1 for upper leaves. Plants responded more favorably to NH₄NO₃ than sole NH₄ ⁺ or NO₃ ^? fertilizers. These results suggest that N fertilization can be used to promote net photosynthesis as well as increase N storage in black walnut seedlings. The NH₄NO₃ appears to be the preferred N source to promote black walnut growth and physiology.     

Evaluation of the sensitivity of a 15N test for nitrogen deficiency in plants

Abstract

A test for nitrogen (N) deficiency in plants showed that rate of uptake of ¹⁵N by excised roots was inversely related to the amount of N supplied to the growing plant. Deficiency was successfully identified from the rate of uptake of ¹⁵NH₄ ⁺ from solution in a two hour period, and this was adopted in the early development of the method. In this study, modifications were tested in a sensitivity analysis, to compare ¹⁵NH₄ ⁺ and ¹⁵NO₃ ^? uptake for different times of immersion and with different washing treatments, to determine the most sensitive method. Common bentgrass (Agrostis capillaris L.), grown in sand culture at a range of N concentrations from deficient to optimum, was the test plant. Although uptake by roots from N deficient treatments was always significantly higher for both ammonium (NH₄) and nitrate (NO₃), the uptake of NH₄ was two to five times higher than that of NO₃. The rate of uptake of both ions was approximately linear over 20 mins, one hour, or two hours. Although a two hour period of immersion would be necessary in some cases to obtain sufficient ¹⁵N in the roots for analysis, it should therefore be possible to interpolate, for direct comparison of rates of uptake by different species, and also with results from similar phosphorus (P) and potassium (K) bioassays, where radioisotope uptake is measured over a shorter time (15 min). The results suggest that the method initially adopted was the most sensitive to identify N deficiency.     

Effect of Raw and NH4+-enriched Zeolite on Nitrogen Uptake by Wheat and Nitrogen Leaching in Soils with Different Textures

Leaching of nutrients in soil can change the surface and groundwater quality. The present study aimed at investigating the effects of raw and ammonium (NH₄⁺)-enriched zeolite on nitrogen leaching and wheat yields in sandy loam and clay loam soils. The treatments were one level of nitrogen; Z₀: (100 kg (N) ha^?1) as urea, two levels of raw zeolite; Z₁:(0.5 g kg^?1 + 100 kg ha^?1) and Z_2: (1 g kg^?1 + 100 kg ha^?1), and two levels of NH₄⁺-enriched zeolite; Z₃: (0.5 g kg^?1 + 80 kg ha^?1) and Z₄: (1 g kg^?1 + 60 kg ha^?1). Wheat grains were sown in pots and, after each irrigation event, the leachates were collected and their nitrate (NO₃^?) and NH₄⁺ contents were determined. The grain yield and the total N in plants were measured after four months of wheat growth. The results indicated that the amounts of NH₄⁺ and NO₃^? leached from the sandy loam soil were more than those from the clay loam soil in all irrigation events. The maximum and minimum concentrations of nitrogen in the drainage water for both soils were observed at control and NH₄⁺-zeolite treatments, respectively. Total N in the plants grown in the sandy loam was higher compared to plants grown in clay loam soil. Also, nitrogen uptake by plants in control and NH₄⁺-zeolite was higher than that of raw-zeolite treatments. The decrease in the amount of N leaching in the presence of NH₄⁺-zeolite caused more N availability for plants and increased the efficiency of nitrogen fertilizers and the plants yield.     

The influence of the form of nitrogen nutrition on uptake and distribution of cadmium in lettuce varieties

Four lettuce (Lactuca saliva L.) varieties ('Benita’, ‘Wendy’, ‘Mirena’, and ‘Jacky') were grown for 43 days in a pH‐controlled complete nutrient solution without cadmium (Cd) or with either 0.01 or 0.03 mg Cd/1 and with NH₄ or NO₃ as the form of nitrogen nutrition. Cadmium did not affect dry matter yield. ‘Wendy’ had a significantly higher total dry matter production when grown on NO₃ compared to NH₄, whereas growth of ‘Mirena’ was best on NH₄ (P<0.05). Dry weights of ‘Benita’ and ‘Jacky’ were not affected by the N source. Cadmium concentrations in shoots (and roots) of plants grown on NH₄ were significantly higher than in plants grown on NO₃. The Zn concentrations in the shoots were also enhanced. The distribution of Cd in the lettuce varieties was independent of the form of N nutrition. It is concluded that the N source directly affects the amount of Cd taken up, without influencing the Cd distribution.     

NUTRIENT UPTAKE KINETICS OF CLOUDBERRY

Mineral fertilization of cloudberry (Rubus chamaemorus) has been inconclusive so far. Nutrient absorption of cloudberry was studied by solution depletion 1) to characterize its nutrient uptake kinetics and 2) to determine its preferred nitrogen (N) form. Two accessions, ‘Fjordgull’ (a female cultivar from Norway) and a local wild clonal selection from ‘Baie-Comeau’ (Canada) were tested. Ammonium (NH₄) and phosphorus (P) absorption presented a two-component kinetic while nitrate (NO₃), potassium (K), and glycine absorption was better fitted with the Michaelis-Menten equation alone. Cloudberry preferences for nitrogen form were in the order: NH₄-N, glycine, NO₃-N. ‘Fjordgull’ exhibited much higher K_m and J_max for P uptake than the ‘Baie-Comeau’ accession, but they exhibited similar NH₄-N uptake kinetics. However, K_m values for all nutrients tested except NH₄-N were higher than reported for other species. Cloudberry does not appear to have evolved efficient absorption kinetics to compensate for the overall very low abundance of nutrients of peatlands.     

Effect of Two Nitrogen Forms on the Growth and Iron Nutrition of Pea Cultivated in Presence of Bicarbonate

ABSTRACT

Two cultivars of pea: ‘PS210713’ (‘PS’), sensitive to iron deficiency, and ‘Marveille de Kelvedon’ (‘MK’), tolerant, were cultivated in controlled climatic conditions during one month, on a nutrient solution containing either nitrate (NO₃ ^?, 4 mM) or ammonium (NH₄ ⁺, 4 mM) and in the presence of bicarbonate (10 mM). The effects of these nitrogen forms on the growth and the mineral nutrition, and especially iron nutrition are analyzed.

The reduction of growth by bicarbonate was approximately 30% in case of NO₃ ^? nutrition in the two cultivars, whereas in ammoniacal treatment the reduction is only 6% and 18% respectively in ‘PS’ and ‘MK’ cultivars.

In presence of bicarbonate, the plant growth is not stimulated by NO₃ ^?relatively to its growth on ammoniacal medium, as often noticed when plants are cultivated on medium without bicarbonate: In presence of this compound, the biomass production of plant pea, was not influenced by the nitrogen forms. The nitric source led to a ferric chlorosis in the sensitive cultivar plants whereas any chlorosis was noted when ammoniacal source was applied. On the other hand, nitric nitrogen form decreased the nitrogen feeding of plants and increased the potassium one, while the effect of the ammoniacal nitrogen form on these nutrients was quite the inverse. In addition, the later increased the allocation of iron towards shoots. Besides, with this nitrogen source there was not accumulation of nitrate in the plant tissues. In nitric feeding case, the nitrate is mainly accumulated in the roots of the two cultivars. It is noticeable that the sensitive cultivar (‘PS’) accumulates three times more nitrate than the tolerant one (‘MK’).

On the level of the whole plant, the iron and phosphorus nutrition seems unaltered by the nitrogen form.     

Growth and nutrition of pansy as influenced by N‐form ratio and temperature

Abstract

Pansy (Viola xwittrockiana Gams.) producers often observe nutrient disorders among plants grown during warm periods (>18°C) of the growing season. These disorders typically are not seen when production temperatures are optimal (≥18°C) even though fertility regimes may remain the same. Our objectives were to assess the effects of temperature and nitrogen (N) fertility on growth and nutrition of pansy. Pansies cultivar ‘Crown White’ were grown until lateral branches had open flowers. Treatments consisted of two temperatures (12 and 22°C) and three NO₃ ^?:NH₄ ⁺ molar % ratios (100:0, 62:38, and 25:75) with a total concentration of 100 mg N L^?1. A modified Hoagland's solution was used with NO₃ ^?‐N supplied as Ca(NO₃)₂ and KNO₃ and with NH₄ ⁺‐N as (NH₄)₂SO₄. Cumulative nutrient absorption and foliar nutrient content were determined when plant lateral branches flowered. Root and shoot growth were limited when NH₄ ⁺ was present in solutions at high ambient air temperature (22°C), but not at low temperature (12°C). Individual absorption and accumulation of plant nutrients varied with N regimes and temperatures. Overall, pansies absorbed more total N, NH₄ ⁺, NO₃ ^?, calcium (Ca), potassium (K), magnesium (Mg), phosphorus (P), zinc (Zn), and less iron (Fe) and manganese (Mn) at 12°C than at 22°C. In addition, absorption of NO₃ ^? by pansy was negligible if any NH₄ ⁺ was present in solutions at 22°C. Results suggest that pansy growers should adjust fertility programs according to production temperatures to avoid possible nutritional disorders and maximize plant growth. If maximum growth is to be obtained in warm temperatures, the use of NH₄ ⁺‐containing fertilizers should be reduced or eliminated. However, the choice of NO₃ ^?:NH₄ ⁺ ratio for nutrition may be less important under cool growing conditions.     

Effect of N‐form on macronutrient and micronutrient concentration and uptake of creeping bentgrass 1

Abstract

Nitrogen‐form effect on nutrient uptake and the subsequent concentration of nutrients in turfgrass plant tissue has not been thoroughly investigated. This study evaluated the effects of clipping regime and N‐form on the tissue concentration of macronutrients and micronutrients and macronutrient uptake in ‘Penncross’ creeping bentgrass (Agrostis palustris Huds.). Turfgrass plugs were grown under greenhouse conditions in a modified Hoagland's solution with a combination of three nutrient solutions (100% NO₃ ^?, 100% NH₄ ⁺, and 50:50 ratio of NH₄ ⁺:NO₃ ^?) and two cutting regimes (cut and uncut). Concentrations of macronutrients and micronutrients were determined for shoot, root and verdure. Nutrient uptake was determined weekly. Uncut NO₃ ^?‐treated plants accumulated higher concentrations of K, Ca, Mg, B and Cu in the shoot tissue; P, K, Ca, Mg, B, Cu, Mn and Zn in the root tissue; and P, Ca, Mg, B, Fe and Mn in the verdure compared to uncut NN₄ ⁺‐treated plants. Nitrate uptake was greater with uncut NO₃ ^?‐treated plants than was NH₄ ⁺ absorption with uncut NH₄ ⁺‐treated plants. Plants grown with the uncut 50:50 treatment adsorbed more NH₄ ⁺ than NO₃ ^?. Plants grown with the uncut NO₃ ^? and 50:50 treatments adsorbed higher amounts of P, K, and Ca compared to the NH₄ ⁺ treatment. The cut NO₃ ^?‐treated plants accumulated higher concentrations of K in the shoot tissue; P, Ca, Mg, B, Cu, Fe and Mn in the root tissue; and B in the verdure than did the cut NH₄ ⁺‐treated plants. Cut NO₃ ^?‐treated plants adsorbed less NO₃ ^? than did cut NH₄ ⁺‐treated plants adsorbed NH₄ ⁺. The cut 50:50 treatment adsorbed more NH₄ ⁺ than NO₃ ^?. Plants grown with NO₃ ^? and 50:50 treatments, under both cutting regimes, resulted in higher concentrations of most macro‐ and micronutrients and greater nutrient uptake compared to the NH₄ ⁺‐treated plants.     

WHEAT RESPONSE TO NITROGEN SOURCE UNDER SALINE CONDITIONS

ABSTRACT

The influence of nitrogen (N) sources on biomass yield and nutrient uptake of wheat (Triticum aestivum L.) under saline conditions was studied in a greenhouse experiment. Six different forms of N {nitrate-N as Ca(NO₃)₂, urea-N [CO(NH₂)₂], ammonium-N as (NH₄)₂SO₄, nitrate-N+urea-N, nitrate-N+ammonium-N and a control (no N fertilizer)} were factorially combined with three levels of salinity to give a total of 18 treatments that were replicated three times. Each of the five levels of applied N was at the rate of 100?kg?ha^?1. The salinity levels (ECe) were 6.2 and 12.1?dSm^?1, denoted as S ₁ and S ₂ and untreated soil (S ₀), respectively. A basal dose of phosphorus (P) and potassium (K) was also applied. Five wheat plants were grown in each pot for six weeks. Data were collected for shoot and root biomass and shoot samples were analyzed for N, P, K, calcium (Ca), magnesium (Mg), sodium (Na), chloride (Cl), and micronutrients contents. Plant growth and nutrient uptake were influenced by both salinity and source of N. As expected, increasing salinity decreased dry matter production of shoot and root, whereas N application increased plant growth across all levels of salinity. The total dry biomass (shoot and root) of wheat was significantly higher in combined N treatments than in single sources. Irrespective of N forms most of the nutrient concentrations in the shoot was increased with increasing level of salinity. Among the fertilizers the concentration of cation was higher in nitrate-treated plants than in other forms of N. Ammonium-N and urea-N tended to inhibit the uptake of cations compared to nitrate-N under saline conditions. The trend for P and Cl concentration was almost opposite to that of cations concentration in the shoot. The uptake of nutrients seemed to be influenced by cation–anion balance in soil-plant system. Nitrogen concentration of shoot was greatly enhanced by all forms of N in the following order: Ni>NiAm>Am>NiUr>Ur>control. The interactive effect of salinity and fertilizer on iron (Fe), manganese (Mn), and zinc (Zn) contents was not consistent. Among the fertilizers the concentration of trace elements in the shoot was also not significantly different. It was concluded that the plant growth and nutrient concentration of shoot could depend upon N source and level of salinity. The mixed application of both ammoniacal and nitrate forms of N could possibly be conducive to plant growth in salt affected soils.     

Influence of ambient acidity on the absorption of NO3 − and NH4 + by tomato punts

Abstract

The effects of ambient acidity on NO₃ ^? and Nh₄ ⁺ absorption by 26‐day‐old tomato plants (Lycopersicon esculentum Mill.) were examined in solution culture. The absorption rate per unit root mass was measured for 6 hr. The NO₃ ^? absorption rate from 0.4 mM NaNO, was 36% greater at pH 4.5 than at pH 6.5. In contrast, the NhY absorption rate was approximately 42% greater at pH 5.5 or 6.5 than at pH 4.5. The presence of equimolar NHr from 0.4 mM NH^NO, decreased the NO, absorption rate at pH 5.5 or 6.5 but did not reduce the rate at pH 4.5. The NO, absorption rate was inhibited less at pH 5.5 when equimolar NHr was supplied from 0.2 mM (NH₄)₂S0₄ as opposed to NH₄NO₃. At pH 5.5, the N0₃ ^? absorption rate increased with increased #OPNH₄#CP₂SO₄ concentration. The presence of equimolar NO₃ ^? supplied as either NaNO₃ or NH₄NO₃ had no effect on the NH₄ ⁺ absorption rate at pH 5.5 or 6.5. However, at pH 4.5, the NH₄ ⁺ absorption rate was slightly reduced from NH₄NO₃ solutions relative to that from a (NH₄)₂S0₄ solution.     

Effect of Nitrogen Status on Salinity Tolerance of Tall Fescue Turf

ABSTRACT

Turfgrass salinity tolerance is usually studied under conditions of non-limiting nutrition, even though most turfgrasses are managed with growth-limiting levels of nitrogen (N). This study examined the effect of N status (replete versus deficient) on salinity tolerance in tall fescue (Festuca arundinacea Schreb.). Additionally, the interactive effects of N status and salinity on tissue ion concentrations were determined. Two cultivars (‘Monarch’ and ‘Finelawn I’) were grown in nutrient solution culture. Treatments included N level (100% or 25% of maximum N demand) and salinity (0, 40, 80, 120 meq L^?1). Salinity reduced leaf growth under high-N conditions, but much less so under low-N conditions. Concentrations of potassium (K), sodium (Na), and chloride (Cl) in the leaf sap were significantly higher in low-N than in high-N plants, indicating that increased salinity tolerance in low-N turf was not due to ion exclusion. These results suggest that efforts to screen turfgrasses for salt tolerance should be conducted using realistic N-fertility levels.     

Comparison of hydroponic solutions for poinsettia nutritional studies 1

Poinsettia cultivars Supjibi and Freedom were grown in eight hydroponic solutions to develop a baseline solution for further nutritional studies. Four solutions contained nitrogen (N) from Ca(NO₃)₂‐4H₂O and KNO₃ (denoted as ‐NH₄) and four contained Ca(NO₃)₂‐4H₂O, KNO₃, nitric acid, and NH₄NO₃ as the N sources (denoted as +NH₄). The four ‐NH₄ and +NH₄ solutions were further divided by an IX or 2X rate of micronutrients [boron (B), copper (Cu), iron (Fe), manganese (Mn), molybdenum (Mo), and zinc (Zn)] (denoted as IX or 2X). A factorial of these four solutions at 2 concentrations (100 mg L¹ of N and potassium (K) and 15 mg L¹ phosphorus (P), or 300 mg L¹ of N and K and 46 mg L^‐1 P) was studied. Greater leaf and stem dry weight for both ‘Supjibi’ and ‘Freedom’ was observed in plants grown with the +NH₄ solutions, with a larger increase occurring with’ Supjibi’. Leaf NH₄‐N content for both cultivars was higher for both the 100 and 300 mg L^‐1 N and K fertilization rates when NH₄‐N was included. The leaf K content was highest for the plants grown with the +NH/2X solution for ‘Supjibi’, for both fertilization rates, and leaf K content increased as the K application rate increased. Results indicate that for nutritional studies with poinsettias, hydroponic solutions should include between 12.5% to 33% of the N in the NH₄ form, a calcium magnesium (Ca:Mg) ratio of 2:1, and a micronutrient concentration of (mg I/¹) 0.5, 0.02, 6.6, 0.5, 0.1, and 0.05, respectively, for B, Cu, Fe, Mn, Mo, and Zn, for adequate plant growth.