Genotypic Differences in Dry Bean Yield and Yield Components as Influenced by Nitrogen Fertilization and Rhizobia

Dry bean (Phaseolus vulgaris L.) is an important legume worldwide and nitrogen (N) is most yield limiting nutrients. A field experiment was conducted for two consecutive years to evaluate response of 15 dry bean genotypes to nitrogen and rhizobial inoculation. The N and rhizobia treatments were (i) control (0 kg N ha^?1), (ii) seed inoculation with rhizobia strains, (iii) seed inoculation with rhizobia strains + 50 kg N ha^?1, and (iv) 120 kg N ha^?1. Straw yield, grain yield, and yield components were significantly influenced by N and rhizobial treatments. Grain yield, straw yield, number of pods m^?2, and grain harvest index were significantly influenced by year, nitrogen + rhizobium, and genotype treatments. Year × Nitrogen + rhizobium × genotype interactions were also significant for these traits. Hence, these traits varied among genotypes with the variation in year and nitrogen + rhizobium treatments. Inoculation with rhizobium alone did not produce maximum yield and fertilizer N is required in combination with inoculation. Based on grain yield efficiency index, genotypes were classified as efficient, moderately efficient, and inefficient in nitrogen use efficiency (NUE). NUE defined as grain produced per unit N applied decreased with increasing N rate. Overall, NUE was 23.17 kg grain yield kg^?1 N applied at 50 kg N ha^?1 and 13.33 kg grain per kg N applied at 120 kg N ha^?1.     

Field-level comparison of nitrogen rates and application methods on maize yield,grain quality and nitrogen use efficiency in a humid environment

Appropriate nitrogen (N) management practices are of critical importance in improving N use efficiency (NUE), maize (Zea mays) yield and environmental quality. A six-year (2005–2010) on-farm trial was conducted in Ottawa, Canada to assess the effects of N rates and application methods on grain yield and NUE. In four out of the six-year study, grain yield increased by 60–77 kg ha^?1 by sidedress, compared to 49–66 kg ha^?1 for each kg N ha^?1 applied at preplant. Grain yield response to N between the two strategies was similar in the other growing seasons. Sidedress strategy required 15 kg N ha^?1 less of the maximum economic rate of N (MERN) than preplant application. Our results indicate that sidedress application of 90–120 kg N ha^?1 with a starter of 30 kg N ha^?1 resulted in greater yield, grain quality and NUE than preplant N application in this cool, humid and short growing-season region.     

Effect of nitrogen fertilizer rate and timing on sorghum productivity in Ethiopian highland Vertisols

Sorghum is cultivated on Vertisols in the Ethiopian Highlands. An experiment was conducted in the Gumara-Maksegnit watershed in 2013 and 2014 to assess the effect of rate and timing of nitrogen fertilizer application on the possibility to shorten the maturity period and to improve the productivity of sorghum. The experiment was laid out as Randomized Complete Block Design with three replications. Treatments were nitrogen doses between 0 and 87 kg N ha^?1 as urea applied at planting, at knee-height stage or in split doses at both stages. Results showed that application of 23, 41, 64 and 87 kg ha^?1 N gave a yield increase of 40, 53, 62 and 69% over the control (0 kg N ha^?1), respectively. In addition, split application of 41 kg ha^?1, 64 kg ha^?1 and 87 kg ha^?1 of nitrogen fertilizer, half at planting and half at knee height stage, gave 19%, 15% and 18% increase in sorghum grain yield over a single dose application, respectively. Applying 87 kg ha^?1 nitrogen fertilizer with split application half at planting and half at knee height stage, along with 46 kg ha^?1 of P₂O_5, gave the highest grain yield and income.     

Contribution of Biofertilizers and Fertilizer Nitrogen to Nutrient Uptake and Yield of Egyptian Winter Wheat

ABSTRACT

Nutrient uptake and grain and straw yield of Egyptian winter wheat (Triticum aestivum L. Merr.) were evaluated for two site-years after the seed inoculation with two biofertilizer products, Phosphorien, containing the phosphorus (P)-solubilizing bacteria Bacillus megatherium, and Nitrobien, containing a combination of nitrogen (N)-fixing bacteria Azotobacter chroococcum and Azospirillum liposerum. Ammonium nitrate and polymer-coated urea fertilizers were applied to plots alone and together with the biofertilizers at rates of either 83 kg N ha^?1 or 186 kg N ha^?1 for comparison. The highest grain yield (5.76–6.74 Mg ha^?1) and straw yield (11.49–13.32 Mg ha^?1) occurred at the highest fertilizer rates with N fertilizer. There was a slight additional increase in grain and straw yields when a biofertilizer was applied along with N fertilizer. A slightly higher grain and straw yield was measured with the polymer-coated urea treatment than with the ammonium nitrate treatment. The biofertilizer materials were not as effective as N fertilizers in producing grain (4.02–4.09 Mg ha^?1) or straw (7.71–8.11 Mg ha^?1) for either year, although the Nitrobien + Phosphorien combination increased these parameters over the N-fertilizer control. The effect of the Nitrobien biofertilizer in increasing grain yields was equivalent to a urea application rate of about 13 kg N ha^?1. Biofertilizer inoculations increased iron (Fe), manganese (Mn), zinc (Zn), and copper (Cu) concentrations in wheat tissue (at boot stage), but these higher levels did not influence grain or straw yield.     

Nitrogen topdressing at the jointing stage affects the nutrient accumulation and translocation in rainfed waxy maize

Timely and fitting nitrogen (N) application decreases costs and pollution risk in maize cultivation. To explore the accumulation and remobilization of dry matter (DM), N, phosphorus (P), and potassium (K) in waxy maize under various N topdressings (0?kg ha^?1, LN; 150?kg ha^?1, MN; 300?kg ha^?1, HN) at the jointing stage, a field trial involving two waxy maize varieties (Suyunuo 5 and Yunuo 7) was conducted in 2013–2016. The highest grain yield was obtained under MN mainly due to the highest grain numbers and grain weight. The increase in grain yield under MN was mainly due to the high DM accumulation post-silking, as well as high N, P, and K accumulation and remobilization pre-silking. Generally, the plants had high harvest index (HI) of DM (N, P, and K), partial N fertilizer productivity, and moderate N utilization efficiency (NUE) under MN.     

Sesbania rostrata green manure and the nitrogen content of rice crop and soil

The effects of four treatments upon the N content of rice crop and soil in 1m² irrigated microplots were compared: (1) PK fertilization + Sesbania rostrata (inoculated stems) ploughed in as green manure when it was 52 days old. (2) PK fertilization + S. rostrata (non-inoculated stems) ploughed in as green manure. (3) PK fertilization + ammonium sulphate (60kg N ha^?1). (4) PK fertilization alone (control).The application of chemical N fertilizer (treatment 3) increased the grain yield by 169 g m^?2 (1.69t ha^?1). whereas incorporating S. rostrata as green manure resulted in a grain yield increase of 372 g m^?2 (3.72t ha^?1). N₂ fixed by S. rostrata was estimated to be at least 26.7 g m^?2 (267kg N ha^?1), one third being transferred to the crop and two thirds to the soil.     

Developing nitrogen management strategies under drip fertigation for wheat and maize production in the North China Plain based on a 3‐year field experiment

The intensive winter wheat (Triticum aestivum L.)–summer maize (Zea mays L.) cropping systems in the North China Plain (NCP) rely on the heavy use of mineral nitrogen (N) fertilizers. As the fertigated area of wheat and maize in the NCP has grown rapidly during recent years, developing N management strategies is required for sustainable wheat and maize production. Field experiments were conducted in Hebei Province during three consecutive growth seasons in 2012–2015 to assess the influence of different N fertigation rates on N uptake, yield, and nitrogen use efficiency [NUE: recovery efficiency (RE_N) and agronomic efficiency (AE_N)]. Five levels of N application, 0 (FN₀), 40 (FN_40%), 70 (FN_70%), 100 (FN_100%), and 130% (FN_130%) of the farmer practice rate (FP: 250 kg N ha^?1 and 205.5 kg N ha^?1 for wheat and maize, respectively), corresponding to 0, 182.2, 318.9, 455.5, and 592.2 kg N ha^?1 y^?1, respectively, were tested. Nitrogen in the form of urea was dissolved in irrigation water and split into six and four applications for wheat and maize, respectively. In addition, the treatment “drip irrigation + 100% N conventional broadcasting” (DN_100%) was also conducted. All treatments were arranged in a randomized complete block design with three replications. The results revealed the significant influence of both N fertigation rate and N application method on grain yield and NUE. Compared to DN_100%, FN_100% significantly increased the 3‐year averaged N recovery efficiency (RE_N) by 0.09 kg kg^?1 and 0.04 kg kg^?1, and the 3‐year averaged N agronomic efficiency (AE_N) by 2.43 kg kg^?1 and 1.62 kg kg^?1 for wheat and maize, respectively. Among N fertigation rates, there was no significant increase in grain yield in response to N applied at a greater rate than 70% of FP due to excess N accumulation in vegetative tissues. Compared to FN_70%, FN_100%, and FN_130%, FN_40% increased the RE_N by 0.17–0.57 kg kg^?1 and 0.03–0.34 kg kg^?1and the AE_N by 4.60–27.56 kg kg^?1 and 2.40–10.62 kg kg^?1 for wheat and maize, respectively. Based on a linear‐response relationship between the N fertigation rate and grain yield over three rotational periods it can be concluded that recommended N rates under drip fertigation with optimum split applications can be reduced to 46% (114.6 kg N ha^?1) and 58% (116.6 kg N ha^?1) of FP for wheat and maize, respectively, without negatively affecting grain yield, thereby increasing NUE.     

Nitrogen Balance in the Magruder Plots Following 109 Years in Continuous Winter Wheat

Abstract

The Magruder plots are the oldest continuous soil fertility wheat research plots in the Great Plains region, and are one of the oldest continuous soil fertility wheat plots in the world. They were initiated in 1892 by Alexander C. Magruder who was interested in the productivity of native prairie soils when sown continuously to winter wheat. This study reports on a simple estimate of nitrogen (N) balance in the Magruder plots, accounting for N applied, N removed in the grain, plant N loss, denitrification, non‐symbiotic N fixation, nitrate (NO₃ ^?) leaching, N applied in the rainfall, estimated total soil N (0–30 cm) at the beginning of the experiment and that measured in 2001. In the Manure plots, total soil N decreased from 6890 kg N ha^?1 in the surface 0–30 cm in 1892, to 3198 kg N ha^?1 in 2002. In the Check plots (no nutrients applied for 109 years) only 2411 kg N ha^?1 or 35% of the original total soil organic N remains. Nitrogen removed in the grain averaged 38.4 kg N ha^?1 yr^?1 and N additions (manure, N in rainfall, N via symbiotic N fixation) averaged 44.5 kg N ha^?1 yr^?1 in the Manure plots. Following 109 years, unaccounted N ranged from 229 to 1395 kg N ha^?1. On a by year basis, this would translate into 2–13 kg N ha^?1 yr^?1 that were unaccounted for, increasing with increased N application. For the Manure plots, the estimate of nitrogen use efficiency (NUE) (N removed in the grain, minus N removed in the grain of the Check plots, divided by the rate of N applied) was 32.8%, similar to the 33% NUE for world cereal production reported in 1999.     

Adjusting Midseason Nitrogen Rate Using a Sensor-Based Optimization Algorithm to Increase Use Efficiency in Corn

ABSTRACT

This study was conducted to formulate an in-season nitrogen (N) fertilization optimization algorithm (NFOA) to estimate midseason N rates that maximize corn (Zea mays L.) growth and minimize fertilizer inputs. Treatments included: a zero kg N ha^?1; three treatments of 134 kg N ha^?1 fixed rate applied in split, preplant, or sidedress; two treatments of 67 kg N ha^?1 fixed rate preplant or sidedress applied; three NFOA-based midseason N rates (RI-NFOA, RICV-NFOA, flat-RICV-NFOA) with (67 kg N ha^?1) and without preplant N; and two resolutions (0.34 and 2.32 m²) tested for RICV-NFOA only. With the 67 kg N ha^?1 preplant application, midseason RI-NFOA-based N rates resulted in an N use efficiency (NUE) of 65% while the 134 kg N ha^?1 fixed rate split applied had 56% NUE. Using the RICV-NFOA, NUE and net returns to N fertilizer were higher when spatial variability was treated at 2.32 m² resolution.     

Nitrogen Rate Influence on Pearl Millet Yield,Nitrogen Uptake,and Nitrogen Use Efficiency in Nebraska

Abstract

Pearl millet is a potential dryland crop for Nebraska. Experiments were conducted in eastern Nebraska in 2000, 2001, and 2002, and in western Nebraska in 2000 and 2001. The objectives were to determine optimum nitrogen (N) rate, N uptake, and N use efficiency (NUE) for pearl millet. The hybrids “68×086R” and “293A×086R” and N rates of 0, 45, 90, and 135 kg N ha^?1 were used. Hybrids had similar yield, N uptake and NUE responses. In western Nebraska in 2000, pearl millet yield response to N rate was linear, but the yield increase was only 354 kg ha^?1 to application of 135 kg N ha^?1. In eastern Nebraska, pearl millet response to N rate was quadratic with maximum grain yields of 4040 in 2001 and 4890 kg ha^?1 in 2002 attained with 90 kg N ha^?1. The optimum N rate for pearl millet was 90 kg N ha^?1 for eastern Nebraska. For western Nebraska, drought may often limit pearl millet's response to N fertilizer.     

Impact of Various Ratios of Nitrogen and Sulfur on Maize and Soil pH in Semiarid Region

Nitrogen and sulfur play an important role in maize production. The aim of this study was to evaluate the effect of nitrogen (N) and sulfur (S) levels applied in various ratios on maize hybrid Babar yield at Peshawar in 2011 and 2013. Four N levels (120, 160, 200 and 240 kg N ha^?1) and four S levels (20, 25, 30 and 35 kg S ha^?1) were applied in three splits: a, at sowing; b, V8 stage; c, VT stage in ratios of 10:50:40, 20:50:30 and 30:50:20. Grains ear^?1, thousand grain weight, grain yield ha^?1 and soil pH were significantly affected by years (Y), N, S and their ratios, while no effect of N, S and their ratios was noted on ears plant^?1. Maximum grains ear^?1 (390), thousand grain weight (230.1 g) and grain yield (4119 kg ha^?1) were recorded in 2013. N increased grains ear^?1 (438), thousand grain weight (252 g) and grain yield (5001 kg ha^?1) up to 200 kg N ha^?1. Each increment of S increased grains ear^?1 and other parameters up to 35 kg S ha^?1, producing maximum grains ear^?1 (430), thousand grain weight (245 g) and grain yield (4752 kg ha^?1), while soil pH decreased from 8.06 to 7.95 with the application of 35 kg S ha^?1. In the case of N and S ratios, more grains ear^?1 (432), heavier thousand grains (246.7 g) and higher grain yield (4806 kg ha^?1) were observed at 30:50:20 where 30% of N and S were applied at sowing, 50% at V8 and 20% at VT stage. It is concluded that 200 kg N ha^?1 and 35 kg S ha^?1 applied in the ratio of 30% at sowing, 50% at V8 and 20% at VT stage is recommended for obtaining a higher yield of maize hybrid Babar.     

Balanced fertilization and bioregulators: enhancing productivity and profitability of wheat (Triticum aestivum)

A field experiment was conducted during the winter seasons between 2004 and 2006 to assess the role of balanced fertilization and bioregulators (foliar-applied brassinosteroid 0.5 mg I^?1, thiourea 1000 mg I^?1 and kinetin 10 mg I^?1) in enhancing the productivity of wheat. Conjoint application of NPKSZn (120 kg N, 40 kg P₂O₅, 30 kg K₂O, 40 kg S, 5.5 kg Zn ha^?1) recorded maximum improvements in yield attributes and significantly out-yielded all the fertilization treatments with 14.90, 4.97 and 6.39% increments in grain yield compared with NPK, NPKS and NPKZn treatments, respectively. Nutrient (N, P, K, S, Zn) content and uptake were also improved significantly with balanced fertilization. Among the bioregulators, application of 0.50 mg I^?1 brassinosteroid recorded maximum increments in grain yield (14.10%), followed by 10 mg I^?1 kinetin (12.31%) and 1000 mg I^?1 thiourea (9.92%), over control (4.99 t ha^?1). Bioregulators significantly enhanced the uptake of nutrients (N, P, K, S, Zn) over control. NPKSZn treatment also gave the maximum net return (Rs. 51,209 ha^?1). Among the bioregulators, brassinosteroid provided the maximum net return (Rs. 47,292 ha^?1) and benefit:cost (B:C) ratio (3.37) followed by thiourea (Rs. 45,500 ha^?1 and 3.35). Kinetin also provided yield advantage, however, it gave a significantly reduced B:C ratio compared with control.     

Optimization of nitrogen fertilizer rate under integrated rice management in a hilly area of Southwest China

China has the world''s highest nitrogen (N) application rate, and the lowest N use efficiency (NUE). With the crop yield increasing, serious N pollution is also caused. An in-situ field experiment (2011-2015) was conducted to examine the effects of three N levels, 0 (i.e., no fertilizer N addition to soil), 120, and 180 kg N ha^-1, using integrated rice management (IRM). We investigated rice yield, aboveground N uptake, and soil surface N budget in a hilly region of Southwest China. Compared to traditional rice management (TRM), IRM integrated raised beds, plastic mulch, furrow irrigation, and triangular transplanting, which significantly improved rice grain yield, straw biomass, aboveground N uptake, and NUE. Integrated rice management significantly improved ¹⁵N recovery efficiency (by 10%) and significantly reduced the ratio of potential ¹⁵N loss (by 8%-12%). Among all treatments, the 120 kg N ha^-1 level under IRM achieved the highest ¹⁵N recovery efficiency (32%) and ¹⁵N residual efficiency (29%), with the lowest ¹⁵N loss ratio (39%). After rice harvest, the residual N fertilizer did not achieve a full replenishment of soil N consumption, as the replenishing effect was insufficient (ranging from -31 to -49 kg N ha^-1). Furthermore, soil surface N budget showed a surplus (69-146 kg N ha^-1) under all treatments, and the N surplus was lower under IRM than TRM. These results indicate IRM as a reliable and stable method for high rice yield and high NUE, while exerting a minor risk of N loss. In the hilly area of Southwest China, the optimized N fertilizer application rate under IRM was found to be 100-150 kg N ha^-1.     

Impact of nitrogen fertilization and tillage practices on nitrous oxide emission from a summer rice ecosystem

ABSTRACT

Identification of the combination of tillage and N fertilization practices that reduce agricultural Nitrous oxide (N₂O) emissions while maintaining productivity is strongly required in the Indian subcontinent. This study investigated the effects of tillage in combination with different levels of nitrogen fertilizer on N₂O emissions from a rice paddy for two consecutive seasons (2013–2014 and 2014–2015). The experiment consisted of two tillage practices, i.e., conventional (CT) and reduced tillage (RT), and four levels of nitrogen fertilizer, i.e., 0 kg N ha^–1 (F1), 45 kg N ha^–1 (F2), 60 kg N ha^–1 (F3) and 75 kg N ha^–1 (F4). Both tillage and fertilizer rate significantly affected cumulative N₂O emissions (p < 0.05). Fertilizer at 45 and 60 kg N ha^–1 in RT resulted in higher N₂O emissions over than did the CT. Compared with the recommended level of 60 kg N ha^?1, a 25% reduction in the fertilizer to 45 kg N ha^?1 in both CT and RT increased nitrogen use efficiency (NUE) and maintained grain yield, resulting in the lowest yield-scaled N₂O-N emission. The application of 45 kg N ha^?1 reduced the cumulative emission by 6.08% and 6% in CT and RT practices, respectively, without compromising productivity.     

Effects of Nitrogen Application Rates on Rice Grain Yield,Nitrogen-Use Efficiency,and Water Quality in Paddy Field

Excessive nitrogen (N) fertilizer application is common in the central Zhejiang Province area, China. A three-year (2009–11) experiment was conducted to determine the optimum N application rate for this area by studying the effects of various N rates on rice (Oryza sativa L.) yield, N-use efficiency (NUE), and quality of paddy field water. Results showed that no significant yield differences were observed under N rates from 180 to 315 kg ha^?1. The NUE could be improved by reducing N application rates without significantly decreasing yield. Due to high ammonia (NH₄⁺-N) and nitrate (NO₃^—N) concentrations, 5–7 days after N application was a critical stage for reducing N pollution. The N rate for the greatest yield was 176 kg ha^?1, accounting for 65 percent of the conventional N rate (270 kg ha^?1). The N-rate reduction in this area may be necessary for maintaining high yield, improving NUE, and reducing environmental pollution.     

Increased plant nitrogen loss with increasing nitrogen applied in winter wheat observed with 15nitrogen

Nitrogen (N) fertilizer is generally the most costly input for winter wheat (Triticum aestivum L.) production. Therefore, it was important to maximize fertilizer use efficiency and minimize N losses to the environment. One of the mechanisms responsible for decreased N use efficiency (NUE) was plant N loss. The objectives of this experiment were to determine fertilizer N recovery in winter wheat when produced for forage and grain, and to quantify potential plant N losses from flowering to maturity in winter wheat. Two long‐term (>25 years) winter wheat (Triticum aestivum L.) N rate fertility experiments (Experiment 222 and Experiment 502) were selected to evaluate ¹⁵N fertilizer recovery. Percent ¹⁵N recovery was determined from all microplots in plant tissue at flowering, in the grain, and straw at harvest and in the soil. Fertilizer N(¹⁵NH₄ ¹⁵NO₃) was applied atratesof 0, 45, 90, and 135kg N ha^‐1 in Experiment 222, and 0, 22, 45, 67, 90, and 112 kg N ha^‐1 in Experiment 502. The ratio ofNO₃ ^‐to NH₄ ⁺ in wheat forage at flowering was positively correlated with estimated plant N loss. Estimated plant N loss (total N uptake in wheat at flowering minus N uptake in the grain and straw at maturity) ranged from a net gain of 12 kg N ha^‐1 to a loss of 42 kg N ha^‐1, and losses increased with increasing N applied.     

Effects of Organic and Inorganic Fertilizers on Soil and Plant Nutrients and Yields of Pearl Millet and Wheat under Semi-arid Inceptisols in India

A study was conducted to assess fertilizer effect on pearl millet–wheat yield and plant-soil nutrients with the following treatments: T₁, control; T₂, 100% nitrogen (N); T₃, 100% nitrogen and phosphorus (NP); T₄, 100% nitrogen, phosphorus and potassium (NPK); T₅, 100% NPK + zinc sulfate (ZnSO₄) at 25 kg ha^?1; T₆, 100% NPK + farmyard manure (FYM) at 10 t ha^?1; T₇, 100% NPK+ verimcompost (VC) at 2.5 tha^?1; T₈, 100% NPK + sulfur (S) at 25 kg ha^?1; T₉, FYM at 10 t ha^?1; T₁₀, VC at 2.5 t ha^?1; T₁₁, 100% NPK + FYM at 10 t ha^?1 + 25 kg S ha^?1 + ZnSO₄ at 25 kg ha^?1; and T₁₂, 150% NPK treatments. Treatments differed significantly in influencing soil-plant nutrients and grain and straw yields of both crops. Grain yield had significant correlation with soil-plant N, P, K, S, and zinc (Zn) nutrients. The study indicated superiority of T₁₁ for attaining maximum pearl millet grain yield (2885 kg ha^?1) and straw yield (7185 kg ha^?1); amounts of N (48.9 kg ha^?1), P (8.8 kg ha^?1), K (26.3 kg ha^?1), S (20.6 kg ha^?1), and Zn (0.09 kg ha^?1) taken up; and amounts of soil N (187.7 kg ha^?1), P (13.7 kg ha^?1), K (242.5 kg ha^?1), S (10.1 kg ha^?1), and Zn (0.70 kg ha^?1). It was superior for wheat with grain yield (5215 kg ha^?1) and straw yield (7220 kg ha^?1); amounts of N (120.7 kg ha^?1), P (13.8 kg ha^?1), K (30 kg ha^?1), S (14.6 kg ha^?1), and Zn (0.18 kg ha^?1) taken up; and maintaining soil N (185.7 kg ha^?1), P (14.5 kg ha^?1), K (250.5 kg ha^?1), S (10.6 kg ha^?1), and Zn (0.73 kg ha^?1). Based on the study, 100% NPK + FYM at 10 tha^?1 + Zn at 25 kg ha^?1 + S at 25 kg ha^?1 could be recommended for attaining maximum returns of pearl millet–wheat under semi-arid Inceptisols.     

Early nitrogen deficiency favors high nitrogen recovery efficiency by improving deeper soil root growth and reducing nitrogen loss in wheat

ABSTRACT

A two-year field and micro-plot ¹⁵N-labelled experiment was conducted under two levels of N application rate (240 and 180 kg N ha^–1) with three basal N application stages [seeding (L0), four-leaf stage (L4), and six-leaf stage (L6)] to investigate the effects of reducing basal N application amount and postponing basal N fertilization period on wheat growth and N use efficiency (NUE). No significant differences were observed in grain yield, root growth and root morphology between the N180L4 and N240L0 treatments, while the root-shoot ratio of N180L4 was significantly improved. Postponing basal N application period increased the residual basal ¹⁵N in soil and reduced basal ¹⁵N loss, and N180L4 treatment favored the highest ¹⁵N recovery efficiency (NRE), mainly due to reduced ¹⁵N loss. Grain yield and basal NRE were significantly positively correlated with root dry weight in deeper soil layers (40–60 cm), and the contribution of root growth to improved grain yield and NRE increased with the downward distribution of the roots. Therefore, postponing the basal N fertilization period under N deficiency promotes deeper root growth during the post-jointing period and increases basal N uptake, as well as reducing basal N loss and increasing grain yield and NUE.     

Agronomic Assessment of Nitrogen Use Efficiency in Spring Wheat and Interrelations with Leaf Greenness Under Field Conditions

Four spring wheat genotypes (Triticum aestivum L.) were grown without (N0 = 0 kg N ha^?1) and under ample (N1 = 250 kg ha^?1) nitrogen (N) fertilizer in field experiments in two seasons. The aim was to assess genotypic variation in N use efficiency (NUE) components and N-related indices during grain filling thus to identify superior wheat genotypes. Leaf chlorophyll (SPAD) readings at crucial growth stages were employed to help differentiate genotypes. Interrelations between yield and N-related indices with SPAD, where also assessed to explain possible pathways of improving NUE early in the growing season. Results showed that genotypic effects on NUE were mostly evident in 2000, a year with drier preanthesis and wetter postanthesis than the normal periods. ‘Toronit’ almost always had the highest biomass yield (BY) and grain yield (GY). Except in 1999 under N0, ‘L94491? showed the highest % grain N concentration (GNC). Genotypes affected SPAD at almost all stages and N fertilization delayed leaf senescence for all genotypes and growth seasons. Correlations between SPAD at different growth stages and GY, N biomass yield at maturity (NBYM) and GNC were significant (P≤ 0.001), positive and strong/very strong (>r = 0.7). N translocation efficiency (NTE) was inversely related to PANU (~r = ? 0.77, P≤ 0.001), suggesting that N after anthesis is being preferentially transported to the ears to meet the N demand of the growing grains. It is concluded that there is still a large potential for increased NUE by improved N recirculation, use of fast and inexpensive crop N monitoring tools and high yielding, N uptake efficient genotypes.

Abbreviations: NUE, Nitrogen use efficiency; SPAD, Minolta SPAD-502 chlorophyll meter, NHI, nitrogen harvest index; HI, Harvest index; NTE, N translocation efficiency from vegetative plant parts to grain; DMTE, dry matter translocation efficiency; CPAY, contribution of pre-anthesis assimilates to yield; PANU, Post-anthesis N uptake, d.a.s., days after sowing, N0, zero (0) kg ha^?1 applied N fertilizer, N1, 250 kg ha^?1 applied N fertilizer.     

NITROGEN NUTRITION ON LEAF CHLOROPHYLL,CANOPY REFLECTANCE,GRAIN PROTEIN AND GRAIN YIELD OF WHEAT VARIETIES WITH CONTRASTING GRAIN PROTEIN CONCENTRATION

Wheat cultivars (‘AC Barrie’, ‘Brook Field’, ‘Hoffman’, and ‘Norwell’) with different protein concentrations were compared under four nitrogen (N) levels (0, 50, 100 and 150 kg ha^?1) in an environment-controlled greenhouse, and the same experiment with an additional N level (200 kg N ha^?1) was repeated in the field in 2007. In the greenhouse experiment, application of 100 kg N ha^?1 resulted in significantly greater grain yield due mainly to higher number of grains per spike and heavier mean grain weight; in the field study, the 150 kg N ha^?1 treatment produced the greatest yield (P<0.01) primarily due to more number of grains per spike. Crude grain protein percentage was increased significantly with each increment of N up to the highest level; however, protein yield (kg ha^?1) increased significantly with fertilizer up to 150 kg N ha^?1. Leaf chlorophyll contents were increased linearly with increment of N levels up to 150 kg ha^?1 both in the greenhouse and field trials while leaf area indices continued to increase up to the highest application rate (200 kg N ha^?1). Canopy reflectance, expressed as normalized difference vegetation index (NDVI), attained maximum value with 150 kg N ha^?1 in the field experiment. Among the varieties tested, “Hoffman” out-yielded other three varieties due to heavier grain weight. Although highest grain and/or plant crude protein content were recorded in ‘AC Barrie’, it was the variety ‘Hoffman’ that produced the highest total protein (kg ha^?1) with largest NDVI and leaf area index (LAI) values.