Water,land, and energy use efficiencies and financial evaluation of air conditioner cooled greenhouses based on field experiments

High temperature and humidity can be controlled in greenhouses by using mechanical refrigeration cooling system such as air conditioner(AC)in warm and humid regions.This study aims to evaluate the techno-financial aspects of the AC-cooled greenhouse as compared to the evaporative cooled(EV-cooled)greenhouse in winter and summer seasons.Two quonset single-span prototype greenhouses were built in the Agriculture Experiment Station of Sultan Qaboos University,Oman,with dimensions of 6.0 m long and 3.0 m wide.The AC-cooled greenhouse was covered by a rockwool insulated polyethylene plastic sheet and light emitting diodes(LED)lights were used as a source of light,while the EV-cooled greenhouse was covered by a transparent polyethylene sheet and sunlight was used as light source.Three cultivars of high-value lettuce were grown for experimentation.To evaluate the technical efficiency of greenhouse performance,we conducted measures on land use efficiency(LUE),water use efficiency(WUE),gross water use efficiency(GWUE)and energy use efficiency(EUE).Financial analysis was conducted to compare the profitability of both greenhouses.The results showed that the LUE in winter were 10.10 and 14.50 kg/m² for the AC-and EV-cooled greenhouses,respectively.However,the values reduced near to 6.80 kg/m² in both greenhouses in summer.The WUE of the AC-cooled greenhouse was higher than that of the EV-cooled greenhouse by 3.8%in winter and 26.8%in summer.The GWUE was used to measure the total yield to the total greenhouse water consumption including irrigation and cooling water;it was higher in the AC-cooled greenhouse than in the EV-cooled greenhouse in both summer and winter seasons by almost 98.0%–99.4%.The EUE in the EV-cooled greenhouse was higher in both seasons.Financial analysis showed that in winter,gross return,net return and benefit-to-cost ratio were better in the EVcooled greenhouse,while in summer,those were higher in the AC-cooled greenhouse.The values of internal rate of return in the AC-and EV-cooled greenhouses were 63.4%and 129.3%,respectively.In both greenhouses,lettuce investment was highly sensitive to changes in price,yield and energy cost.The financial performance of the AC-cooled greenhouse in summer was better than that of the EV-cooled greenhouse and the pattern was opposite in winter.Finally,more studies on the optimum LED light intensity for any particular crop have to be conducted over different growing seasons in order to enhance the yield quantity and quality of crop.     

Identifying water vapor sources of precipitation in forest and grassland in the north slope of the Tianshan Mountains,Central Asia

Identifying water vapor sources in the natural vegetation of the Tianshan Mountains is of significant importance for obtaining greater knowledge about the water cycle, forecasting water resource changes, and dealing with the adverse effects of climate change. In this study, we identified water vapor sources of precipitation and evaluated their effects on precipitation stable isotopes in the north slope of the Tianshan Mountains, China. By utilizing the temporal and spatial distributions of preci...     

Effect of the W-beam central guardrails on wind-blown sand deposition on desert expressways in sandy regions

Many desert expressways are affected by the deposition of the wind-blown sand,which might block the movement of vehicles or cause accidents.W-beam central guardrails,which are used to improve the safety of desert expressways,are thought to influence the deposition of the wind-blown sand,but this has yet not to be studied adequately.To address this issue,we conducted a wind tunnel test to simulate and explore how the W-beam central guardrails affect the airflow,the wind-blown sand flux and the deposition of the wind-blown sand on desert expressways in sandy regions.The subgrade model is 3.5 cm high and 80.0 cm wide,with a bank slope ratio of 1:3.The W-beam central guardrails model is 3.7 cm high,which included a 1.4-cm-high W-beam and a 2.3-cm-high stand column.The wind velocity was measured by using pitot-static tubes placed at nine different heights(1,2,3,5,7,10,15,30 and 50 cm)above the floor of the chamber.The vertical distribution of the wind-blown sand flux in the wind tunnel was measured by using the sand sampler,which was sectioned into 20 intervals.In addition,we measured the wind-blown sand flux in the field at K50 of the Bachu-Shache desert expressway in the Taklimakan Desert on 11 May 2016,by using a customized 78-cm-high gradient sand sampler for the sand flux structure test.Obstruction by the subgrade leads to the formation of two weak wind zones located at the foot of the windward slope and at the leeward slope of the subgrade,and the wind velocity on the leeward side weakens significantly.The W-beam central guardrails decrease the leeward wind velocity,whereas the velocity increases through the bottom gaps and over the top of the W-beam central guardrails.The vertical distribution of the wind-blown sand flux measured by wind tunnel follows neither a power-law nor an exponential function when affected by either the subgrade or the W-beam central guardrails.At 0.0H and 0.5H(where H=3.5 cm,which is the height of the subgrade),the sand transport is less at the 3 cm height from the subgrade surface than at the 1 and 5 cm heights as a result of obstruction by the W-beam central guardrails,and the maximum sand transportation occurs at the 5 cm height affected by the subgrade surface.The average saltation height in the presence of the W-beam central guardrails is greater than the subgrade height.The field test shows that the sand deposits on the overtaking lane leeward of the W-beam central guardrails and that the thickness of the deposited sand is determined by the difference in the sand mass transported between the inlet and outlet points,which is consistent with the position of the minimum wind velocity in the wind tunnel test.The results of this study could help us to understand the hazards of the wind-blown sand onto subgrade with the W-beam central guardrails.     

Optimal bandwidth selection for retrieving Cu content in rock based on hyperspectral remote sensing

Hyperspectral remote sensing technology is widely used to detect element contents because of its multiple bands,high resolution,and abundant information.Although researchers have paid considerable attention to selecting the optimal bandwidth for the hyperspectral inversion of metal element contents in rocks,the influence of bandwidth on the inversion accuracy are ignored.In this study,we collected 258 rock samples in and near the Kalatage polymetallic ore concentration area in the southwestern part of Hami City,Xinjiang Uygur Autonomous Region,China and measured the ground spectra of these samples.The original spectra were resampled with different bandwidths.A Partial Least Squares Regression(PLSR)model was used to invert Cu contents of rock samples and then the influence of different bandwidths on Cu content inversion accuracy was explored.According to the results,the PLSR model obtains the highest Cu content inversion accuracy at a bandwidth of 35 nm,with the model determination coefficient(R²)of 0.5907.The PLSR inversion accuracy is relatively unaffected by the bandwidth within 5-80 nm,but the accuracy decreases significantly at 85 nm bandwidth(R²=0.5473),and the accuracy gradually decreased at bandwidths beyond 85 nm.Hence,bandwidth has a certain impact on the inversion accuracy of Cu content in rocks using the PLSR model.This study provides an indicator argument and theoretical basis for the future design of hyperspectral sensors for rock geochemistry.     

Assessing the collapse risk of Stipa bungeana grassland in China based on its distribution changes

The criteria used by International Union for Conservation of Nature(IUCN) for its Red List of Ecosystems(RLE) are the global standards for ecosystem-level risk assessment, and they have been increasingly used for biodiversity conservation. The changed distribution area of an ecosystem is one of the key criteria in such assessments. The Stipa bungeana grassland is one of the most widely distributed grasslands in the warm-temperate semi-arid regions of China. However, the total distribution area of this grassland was noted to have shrunk and become fragmented because of its conversion to cropland and grazing-induced degradation. Following the IUCN-RLE standards, here we analyzed changes in the geographical distribution of this degraded grassland, to evaluate its degradation and risk of collapse. Past(1950-1980) distribution areas were extracted from the Vegetation Map of China(1:1,000,000). Present realizable distribution areas were equated to these past areas minus any habitat area losses. We then predicted the grassland’s present and future(under the Representative Concentration Pathway 8.5 scenario) potential distribution areas using maximum entropy algorithm(MaxEnt), based on field survey data and nine environmental layers. Our results showed that the S. bungeana grassland was mainly distributed in the Loess Plateau, Hexi Corridor, and low altitudes of the Qilian Mountains and Longshou Mountain. This ecosystem occurred mainly on loess soils, kastanozems, steppe aeolian soils and sierozems. Thermal and edaphic factors were the most important factors limiting the distribution of S. bungeana grassland across China. Since 56.1% of its past distribution area(4.9×10~4 km^2) disappeared in the last 50 a, the present realizable distribution area only amounts to 2.2×10~4 km^2. But only 15.7% of its present potential distribution area(14.0×10~4 km^2) is actually occupied by the S. bungeana grassland. The future potential distribution of S. bungeana grassland was predicted to shift towards northwest, and the total area of this ecosystem will shrink by 12.4% over the next 50 a under the most pessimistic climate change scenario. Accordingly, following the IUCN-RLE criteria, we deemed the S. bungeana grassland ecosystem in China to be endangered(EN). Revegetation projects and the establishment of protected areas are recommended as effective ways to avert this looming crisis. This empirical modeling study provides an example of how IUCN-RLE categories and criteria may be valuably used for ecosystem assessments in China and abroad.     

conomic losses from reduced freshwater under future climate scenarios: An example from the Urumqi River,Tianshan Mountains

As important freshwater resources in alpine basins,glaciers and snow cover tend to decline due to climate warming,thus affecting the amount of water available downstream and even regional economic development.However,impact assessments of the economic losses caused by reductions in freshwater supply are quite limited.This study aims to project changes in glacier meltwater and snowmelt of the Urumqi River in the Tianshan Mountains under future climate change scenarios(RCP2.6(RCP,Representative Concentration Pathway),RCP4.5,and RCP8.5)by applying a hydrological model and estimate the economic losses from future meltwater reduction for industrial,agricultural,service,and domestic water uses combined with the present value method for the 2030 s,2050 s,2070 s,and 2090 s.The results indicate that total annual glacier meltwater and snowmelt will decrease by 65.6%and 74.5%under the RCP4.5 and RCP8.5 scenarios by the 2090 s relative to the baseline period(1980-2010),respectively.Compared to the RCP2.6 scenario,the projected economic loss values of total water use from reduced glacier meltwater and snowmelt under the RCP8.5 scenario will increase by 435.10×10⁶ and 537.20×10⁶ CNY in the 2050 s and 2090 s,respectively,and the cumulative economic loss value for 2099 is approximately 2124.00×10⁶ CNY.We also find that the industrial and agricultural sectors would likely face the largest and smallest economic losses,respectively.The economic loss value of snowmelt in different sectorial sectors is greater than that of glacier meltwater.These findings highlight the need for climate mitigation actions,industrial transformation,and rational water allocation to be considered in decision-making in the Tianshan Mountains in the future.     

Coupling between the Grain for Green Program and a household level-based agricultural eco-economic system in Ansai,Shaanxi Province of China

The implementation of the Grain for Green Program(GGP)has changed the development track of the agricultural eco-economic system in China.In response to the results of a lag study that investigated the coupling between the GGP and the agricultural eco-economic system in a loess hilly region,we used a structural equation model to analyze the survey data from 494 households in Ansai,a district of Yan’an City in Shaanxi Province of China in 2015.The model clarified the direction and intensity of the coupling between the GGP and the agricultural eco-economic system.The coupling benefits were derived through linkages between the program and various chains in the agricultural eco-economic system.The GGP,the agroecosystem of Ansai and their potential coupling effects were in a state of general coordination.The agroecosystem directly affected the coupling effect,with the standardized path coefficient of 0.87,indicating that the agroecosystem in Ansai at this stage provided basic material support for the coupling between the GGP and the agricultural eco-economic system.The direct path coefficient of agroeconomic system impacted on the coupling effect was-0.76,indicating that partial contradictions occurred between the agroeconomic system and the coupling effect.Therefore,although the current agroecosystem in Ansai should be provided sufficient agroecological resources for the benign coupling between the program and the agricultural eco-economic system,agricultural development failed to effectively transform agroecological resources into agricultural economic advantages in this region,which resulted in a relative lag in the development of the agricultural economic system.Thus,the coupling between the GGP and the agricultural eco-economic system was poor.To improve the coupling and the sustainable development of the agricultural eco-economic system in cropland retirement areas,the industrial structure needs to be diversified,the agricultural resources(including agroecological resources,agricultural economic resources and agricultural social resources)need to be rationally allocated,and the chain structure of the agricultural eco-economic system needs to be continuously improved.     

Effects of long-term warming on the aboveground biomass and species diversity in an alpine meadow on the Qinghai-Tibetan Plateau of China

Ecosystems in high-altitude regions are more sensitive and respond more rapidly than other ecosystems to global climate warming.The Qinghai-Tibet Plateau(QTP)of China is an ecologically fragile zone that is sensitive to global climate warming.It is of great importance to study the changes in aboveground biomass and species diversity of alpine meadows on the QTP under predicted future climate warming.In this study,we selected an alpine meadow on the QTP as the study object and used infrared radiators as the warming device for a simulation experiment over eight years(2011-2018).We then analyzed the dynamic changes in aboveground biomass and species diversity of the alpine meadow at different time scales,including an early stage of warming(2011-2013)and a late stage of warming(2016-2018),in order to explore the response of alpine meadows to short-term(three years)and long-term warming(eight years).The results showed that the short-term warming increased air temperature by 0.31℃and decreased relative humidity by 2.54%,resulting in the air being warmer and drier.The long-term warming increased air temperature and relative humidity by 0.19℃and 1.47%,respectively,and the air tended to be warmer and wetter.The short-term warming increased soil temperature by 2.44℃and decreased soil moisture by 12.47%,whereas the long-term warming increased soil temperature by 1.76℃and decreased soil moisture by 9.90%.This caused the shallow soil layer to become warmer and drier under both short-term and long-term warming.Furthermore,the degree of soil drought was alleviated with increased warming duration.Under the long-term warming,the importance value and aboveground biomass of plants in different families changed.The importance values of grasses and sedges decreased by 47.56%and 3.67%,respectively,while the importance value of weeds increased by 1.37%.Aboveground biomass of grasses decreased by 36.55%,while those of sedges and weeds increased by 8.09%and 15.24%,respectively.The increase in temperature had a non-significant effect on species diversity.The species diversity indices increased at the early stage of warming and decreased at the late stage of warming,but none of them reached significant levels(P>0.05).Species diversity had no significant correlation with soil temperature and soil moisture under both short-term and long-term warming.Soil temperature and aboveground biomass were positively correlated in the control plots(P=0.014),but negatively correlated under the long-term warming(P=0.013).Therefore,eight years of warming aggravated drought in the shallow soil layer,which is beneficial for the growth of weeds but not for the growth of grasses.Warming changed the structure of alpine meadow communities and had a certain impact on the community species diversity.Our studies have great significance for the protection and effective utilization of alpine vegetation,as well as for the prevention of grassland degradation or desertification in high-altitude regions.     

Characteristics and hazards of different snow avalanche types in a continental snow climate region in the Central Tianshan Mountains

Snow avalanches are a common natural hazard in many countries with seasonally snow-covered mountains.The avalanche hazard varies with snow avalanche type in different snow climate regions and at different times.The ability to understand the characteristics of avalanche activity and hazards of different snow avalanche types is a prerequisite for improving avalanche disaster management in the mid-altitude region of the Central Tianshan Mountains.In this study,we collected data related to avalanche,snowpack,and meteorology during four snow seasons(from 2015 to 2019),and analysed the characteristics and hazards of different types of avalanches.The snow climate of the mid-altitude region of the Central Tianshan Mountains was examined using a snow climate classification scheme,and the results showed that the mountain range has a continental snow climate.To quantify the hazards of different types of avalanches and describe their situation over time in the continental snow climate region,this study used the avalanche hazard degree to assess the hazards of four types of avalanches,i.e.,full-depth dry snow avalanches,full-depth wet snow avalanches,surface-layer dry snow avalanches,and surface-layer wet snow avalanches.The results indicated that surface-layer dry snow avalanches were characterized by large sizes and high release frequencies,which made them having the highest avalanche hazard degree in the Central Tianshan Mountains with a continental snow climate.The overall avalanche hazard showed a single peak pattern over time during the snow season,and the greatest hazard occurred in the second half of February when the snowpack was deep and the temperature increased.This study can help the disaster and emergency management departments rationally arrange avalanche relief resources and develop avalanche prevention strategies.     

Transformation of vegetative cover on the Ustyurt Plateau of Central Asia as a consequence of the Aral Sea shrinkage

The gradual shrinkage of the Aral Sea has led to not only the degradation of the unique environments of the Aral Sea,but also numerous and fast developing succession processes in the neighborhood habitats surrounding the sea.In this study,we investigated the vegetative succession processes related to the Aral Sea shrinkage in the Eastern Cliff of the Ustyurt Plateau in Republic of Uzbekistan,Central Asia.We compared the results of our current investigation(2010–2017)on vegetative communities with the geobotany data collected during the 1970s(1970–1980).The results showed great changes in the mesophytic plant communities and habitat aridization as a result of the drop in the underground water level,which decreased atmospheric humidity and increased the salt content of the soil caused by the shrinkage of the Aral Sea.In the vegetative communities,we observed a decrease in the Margalef index(DMg),which had a positive correlation with the poly-dominance index(I-D).The main indications of the plant communities'transformation were the loss of the weak species,the appearance of new communities with low species diversity,the stabilization of the projective cover of former resistant communities,as well as the appearance of a new competitive species,which occupy new habitats.     

诱虫作物在害虫治理中的应用

诱虫作物主要从物理特性和化学特性两个方面形成比主栽作物更强的引诱力,使主栽作物得以保护。诱虫作物在生产中主要用来诱集害虫、给天敌提供避难和繁殖场所,其作用效果与诱虫作物的品种、播期和田间管理、害虫行为、对天敌的调控、与其他害虫防治方法的有机配合等有重要关系。采用引诱-排斥多策略相结合的方法,研究利用对害虫同时具有诱集和杀灭作用的植物作为诱虫作物,将大大增强诱虫作物在害虫治理中应用的可行性。     

Advances in insect phototaxis and application to pest management: a review

Many insects, especially nocturnal insects, exhibit positive phototaxis to artificial lights. Light traps are currently used to monitor and manage insect pest populations, and play a crucial role in physical pest control. Efficient use of light traps to attract target insect pests is an important topic in the application of integrated pest management (IPM). Phototactic responses of insects vary among species, light characteristics and the physiological status of the insects. In addition, light can cause several biological responses, including biochemical, physiological, molecular and fitness changes in insects. In this review, we discuss several hypotheses on insect phototaxis, factors affecting insect phototaxis, insect‐sensitive wavelengths, biological responses of insects to light, and countermeasures for conserving beneficial insects and increasing the effect of trapping. In addition, we provide information on the different sensitivities to wavelengths causing positive phototactic behavior in > 70 insect pest and beneficial insect species. The use of advanced light traps equipped with superior light sources, such as light‐emitting diodes (LEDs), will make physical pest control in IPM more efficient. © 2019 Society of Chemical Industry     

昆虫对双酰胺类杀虫剂抗性机制研究进展

双酰胺类杀虫剂是以昆虫鱼尼丁受体为作用靶标的新型杀虫剂,由于其作用机制独特,对多种鳞翅目害虫具有良好的防治效果而得到广泛应用。但已经有多种害虫的田间种群对该类药剂产生了抗性,甚至导致田间防治失败。本文在综述昆虫对双酰胺类杀虫剂抗性现状的基础上,重点总结了抗性机制方面的最新研究进展,并对今后的研究方向进行了展望,以期为进一步深入研究双酰胺类杀虫剂的抗性机制提供借鉴。     

稻田中性昆虫多样性及其生态功能分析

以广东省四会市大沙镇水稻害虫综合防治稻作区为试验基地 ,用吸虫器法采样 ,对稻田节肢动物群落中的中性昆虫、捕食性天敌、寄生性天敌与害虫进行了系统调查。在整个稻田节肢动物群落中 ,稻田中性昆虫物种丰富度的比例 ,在早稻和晚稻分别为 1 6 .98%和 6 .82 % ;而其数量比例为 :早稻 73.6 1 %、晚稻 37.94 %。稻田中捕食性天敌亚群落的发生与重建比水稻害虫亚群落发展快 ,前者在水稻生长发育前期主要依赖于稻田中的中性昆虫重建其亚群落     

中国大豆病虫害发生现状及全程绿色防控技术研究进展

病虫害频发是制约大豆高产稳产的重要因素之一。中国大豆生产中已知病虫害近500种,产生重大危害的有50余种,造成的大豆产量损失一般为15%～30%,严重时甚至大面积绝收。该文综述了中国大豆生产中主要病虫害的种类、大豆主要产区病虫害的发生现状和全程绿色防控技术的相关研究进展,探讨了当前大豆病虫害防控中存在的问题,并对未来发展方向进行展望,以期为后续开展大豆病虫害监测与绿色防控关键技术的研究与应用提供借鉴。     

昆虫不育技术在害虫防治中的应用和研究进展

昆虫不育技术是一种通过释放不育昆虫来控制田间害虫种群的生物防治新策略。为将昆虫不育技术应用于防治全球入侵害虫草地贪夜蛾Spodoptera frugiperda,该文对昆虫不育技术的作用机理和防治现状进行了综述,并对国内外利用该技术成功防治害虫的作用原理、作用方式和对多种害虫防治的成功案例进行了归纳;同时,对防治靶标基因doublesex的功能性作用进行总结。本综述基于CRISPR/Cas9基因编辑系统调控靶标基因,整理并展望通过释放携带显性致死基因的昆虫种群实现虫口密度控制的研究现状,为促进昆虫不育技术防治害虫提供理论基础。     

高毒力杀虫真菌基因工程菌株选育的进展与方向

病原真菌主要通过体壁侵染昆虫,在害虫防治中具有重要作用.利用昆虫病原真菌研制杀虫真菌农药的主要技术难点是选育高毒力杀虫真菌工程菌,本文综述了高毒力杀虫真菌工程菌构建技术的现状和菌株选育的进展,分析了其中存在的问题.根据昆虫病原真菌致病机制、害虫新靶标发现等方面的研究进展,提出了高毒力杀虫真菌工程菌选育技术的发展方向.     

Killing two bugs with one stone: a perspective for targeting multiple pest species by incorporating reproductive interference into sterile insect technique

The sterile insect technique is an environmentally friendly method to control and even eradicate agricultural and veterinary insect pests without using chemical pesticides in excess. However, the continuous production and release of sterile insects is economically costly and eradication programs using sterile insects have not always been successful owing to the incomplete mating ability of the sterile insects. Here we focus on the theory and empirical findings of interspecific negative mating interaction, known as reproductive interference, to develop a more cost‐effective and value‐added pest management program. We suggest that sterile insects can be used for simultaneous control of both wild‐type conspecifics and closely related pest species by taking advantage of the fact that, when species recognition abilities are incomplete, courtship and mating are often misdirected toward heterospecies. This new approach might help mitigate economic damage and human health crises caused by pest insects. © 2018 Society of Chemical Industry     

海南省椰子病虫害的监测与调查

通过近2年来对海南省椰子作物病虫害的野外监测、调查和室内鉴定的结果表明,目前在海南省椰子作物上发生的病虫共有37种,其中害虫30种,病害7种。主要病虫种类包括椰心叶甲(BrontispalongissimaGestro)、红棕象甲(Rhynchophorus ferrugineus Fabricius)、二疣犀甲(Oryctes rhinocerosL.)、椰花四星象甲(Diocalandra frumenti Fabricius)、红脉穗螟(Tirathaba rufivena Walker)、椰子泻血病〔Ceratocystis para-doxa(Dade)Mor〕、椰子灰斑病(Pestalotia palmarum Cooke)、椰子芽腐病(Phytophthora palmivora Butler)和椰子干腐病(Paecilomyces varioti Bainier)等,其中椰心叶甲、红棕象甲、二疣犀甲、椰花四星象甲和椰子泻血病对海南椰子树危害最为严重,应重点予以防控。     

Insecticide toxicity and synergism by enzyme inhibitors in 18 species of pest insect and natural enemies in crucifer vegetable crops

The toxicities of three enzyme inhibitors and their synergistic effects on four insecticides were studied by using the dry film method on field populations of 18 species of insects collected in Jianxin and Shanjie, China, from 2003 to 2005. Meanwhile, the inhibitory effects of these enzyme inhibitors on the activities of acetylcholinesterases (AChE), carboxyesterases (CarE) and glutathione-S-transferases (GST), in vivo, were also studied. In general, triphenyl phosphate (TPP) and diethyl maleate (DEM) showed low toxicities to six herbivorous pest insects, four ladybirds and eight parasitoids. Piperonyl butoxide (PB) exhibited low toxicities to the herbivorous pest insects and ladybirds, but high toxicities to the eight parasitoids. The tolerance to the insecticides in 11 pest insects and natural enemies was mainly associated with the tolerance to PB. PB showed the highest synergism on methamidophos, fenvalerate, fipronil and avermectin in nine species of pest insects and natural enemies. In general, TPP and DEM showed significant synergisms to these four insecticides in four parasitoid species. However, in contrast to their effects on the parasitoids, the synergistic effects of TPP and DEM on the four insecticides by TPP and DEM against four pest insects and one ladybird varied depending on the insect species and enzyme inhibitor. Activity of AChE, CarE or GST could be strongly inhibited, in vivo, by PB, TPP or DEM, depending on the insect species and enzyme inhibitors. From the results obtained in this study, mixed-function oxidase (MFO) was thought to play the most critical role in insect tolerances to the tested insecticides in the field. Low competition existed in the evolution of insecticide resistance in the field populations of parasitoids, as compared with herbivorous pest insects and ladybirds. Possible causes of the high synergistic effects of PB on the four classes of insecticides, based on multiattack on the activity of CarE, GST or AChE in the insect species, are also discussed.