山羊红细胞免疫功能的研究及意义

应用红细胞Ｃ３ｂ受体花环试验和红细胞免疫复合物花环境试验，对山羊红细胞免疫功能进行了初步研究。观察到山羊细胞可形成Ｃ３ｂ受体花环及免疫复合物花环，说明山羊红细胞具有Ｃ３ｂ受体（ＣＲ１），在生理及病理条件下发挥重要的免疫机能，支持了ＲＣＩＳ学说明，本文还对Ｃ３ｂＲＲ和ＩＣＲ所代表的ＣＲ１生物化学状态进行了推断，并对红细胞免疫系统研究的的最新进展进行了报道。     

红细胞免疫功能在家禽中的应用研究进展

红细胞免疫功能是一个较新的研究领域。1981年I.siege1发现了红细胞的多种免疫功能,并预见了血清中存在着红细胞免疫调节系统及红细胞杀伤病原的作用。“红细胞免疫系统”的新概念,更新了人们对红细胞功能的认识,使红细胞免疫研究得到了迅速发展。一红细胞免疫的分子生物学基础     

红细胞免疫功能的研究进展及其应用

红细胞不仅具有运输氧气和二氧化碳的呼吸功能,而且还具有重要的免疫功能.红细胞是机体非特异免疫系统的重要组成部分,还参与调节机体的特异性和非特异性免疫反应.本文综述了红细胞的免疫功能、检测方法、影响红细胞免疫的因素及红细胞免疫在动物医学上的应用.     

双峰驼红细胞免疫功能测定

应用红细胞Ｃ３ｂ受体（ＲＢＣ－Ｃ３ｂＲ）花环和红细胞免疫复合物（ＲＢＣ－ＩＣ）花环试验，对３３峰双峰驼红细胞免疫功能检测证实，双峰驼红细胞膜上具有Ｃ３ｂ受体，ＲＢＣ－Ｃ３ｂＲ花环率为２．７１±１．７２％，ＲＢＣ－ＩＣ花环率为２．３３±１．７５％。说明双峰驼红细胞具有免疫功能，同时，红细胞免疫系统（ＲＣＩＳ）概念亦适用于双峰驼。     

中草药添加剂对鹌鹑红细胞免疫功能影响的研究

1981年,提出了红细胞免疫系统的假说,认为红细胞不仅具有呼吸功能,而且具有识别、粘附、杀伤抗原、清除免疫复合物(CIC)的功能[1],同时还得出试验结论,凡影响免疫功能的疾病,其红细胞上C3b受体的数量都出现明显的减少,其原因是病原     

红细胞在肿瘤免疫中的作用与地位

1981年Siegel提出了红细胞免疫系统（red cellimmunesystem）的新概念，开辟了机体免疫系统的新领域。在肿瘤的发生发展过程中，机体的免疫功能起着非常重要的作用，过去的研究多只涉及白细胞免疫系统，而红细胞在其中所起的作用一直受到忽视，因而对红细胞的作用与地位有必要加以重新认识。     

含硒型牛副伤寒活疫苗对牦牛红细胞免疫功能的影响

Siegel等在前人研究的基础上提出了红细胞免疫系统（RCIS）的新概念,并认为RCIS概念至少适用于哺乳动物,而非灵长类动物所特有,这一概念引起了医学界的广泛关注。郭峰等也发现了人红细胞上存在C3bR,并建立了检测方法。在鸡、兔等动物也发现了红细胞C3bR。硒作为一种日益关注的免疫增强剂,有利于增强机体的免疫功能和红细胞免疫功能。该研究旨在通过含硒型牛副伤寒活疫苗免疫牦牛后,探讨其对牦牛红细胞免疫功能的影响。     

蓝孔雀红细胞免疫功能的研究及意义探讨

红细胞在固有免疫反应及参与适应性免疫反应中都具有重要的作用，应用红细胞C3b受体花环（C3bRR）和免疫复合物花环（ICRR）试验。首次证实蓝孔雀红细胞膜上也有C3b受体，可借助CR1进行免疫黏附，表现出重要的免疫功能，从而证明蓝孔雀的红细胞具有免疫功能，表明红细胞免疫系统（Red cell immune system，RCIS）概念适用于蓝孔雀。研究结果显示，蓝孔雀红细胞C3bRR率及ICRR率分别为50．986±5．527和20．654±2．342，C3bRR率和ICRR率之间差异极显著（P〈0．01），结果与大多数禽类和哺乳动物相似。通过对两种花环的形成及其临床诊断价值进行探讨，以便为红细胞免疫系统（RCIS）的深入研究和应用提供参考。     

樱桃谷鸭红细胞免疫功能的研究及其意义

应用红细胞Ｃ３ｂ受体花环和免疫复合物花环试验，证实樱桃谷鸭红细胞表面存在补体Ｃ３ｂ受体，表明红细胞免疫系统的概念亦适用于这种动物。     

雏鸡红细胞免疫功能发育变化及日粮添加免疫生长促进剂对其的影响

机体的免疫系统是由免疫细胞和免疫分子组成的一个完整的免疫网络,红细胞免疫系统是其中不可缺少的一个重要组成部分〔１〕。由于新生雏鸡免疫生理的特殊性,各种应激因素易使雏鸡潜伏感染转化为活动感染〔２〕,因此雏鸡的免疫功能调节应着手于如何增强机体免疫性,这对...     

电感耦合等离子-质谱法测定饲料中钠、镁、铬、锰、铁、铜、锌、砷、硒、镉和铅

应用电感耦合等离子-质谱技术(ICP-MS),建立饲料中钠、镁、铬、锰、铁、铜、锌、砷、硒、镉和铅等元素的测定方法。对饲料样品的前处理方法、仪器工作参数和11种元素标准曲线进行优化;并以加标回收、分析方法比对和重复测试说明方法的准确性和精密性。方法在0～1 000 ng/ml范围内线性良好,仪器检出限为0.557 7～5.072 ng/ml,具有良好的精密度,其回收率在88.1%～104.4%之间,相对标准偏差小于5.0%。同时与原子吸收和原子荧光方法进行比对,测定结果相近。所建立的方法简单、快速,可替代原子吸收和原子荧光方法测定饲料中的11种金属元素,为饲料的质量控制提供理想的元素分析方法。     

Supplementation of broodmares with copper, zinc, iron, manganese, cobalt, iodine, and selenium

In experiment 1, 6 pregnant mares received a concentrate that contained a trace mineral premix that provided 14.3 mg Cu, 40 mg Zn, 28 mg Fe, 28 mg Mn, 0.08 mg Co, 0.16 mg I, and 0.16 mg Se/kg concentrate (group A). Seven mares received the same concentrate plus 502 mg Zn and 127 mg Cu once daily (group B). No differences (P > .05) in foal growth data, or Cu, Zn, and Fe concentrations of mare milk, mare serum, or foal serum were observed. In experiment 2, 6 pregnant mares received the same concentrate as group A (group C), and 8 mares received the same concentrate fortified with 4× the trace mineral premix (group D). Group C mares had higher serum Zn concentration at 1 day (P < 0.01) and 56 days (P < 0.04). Group C mares had higher milk Fe concentration at 28 days (P < .01), and group D mares had higher milk Cu concentration at 56 days (P < .01). Group C foals had higher serum Cu concentration at 14 days (P < .03). The results from this study provide no evidence to indicate that supplementing late gestating and lactating mares with higher dietary trace mineral levels than those recommended currently by NRC has any influence on foal growth and development, or on the Cu, Zn, and Fe concentrations of the mare milk, mare serum, or foal serum.     

Comparisons of Angus, Charolais, Galloway, Hereford, Longhorn, Nellore, Piedmontese, Salers, and Shorthorn breeds for weight, weight adjusted for condition score, height, and condition score of cows

Breed differences for weight (CW), height (CH), and condition score (CS) were estimated from records (n = 12,188) of 2- to 6-yr-old cows (n = 744) from Cycle IV of the U.S. Meat Animal Research Center's Germplasm Evaluation (GPE) Program. Cows were produced from mating Angus and Hereford dams to Angus, Hereford, Charolais, Shorthorn, Galloway, Longhorn, Nellore, Piedmontese, and Salers sires. Samples of Angus and Hereford sires were 1) reference sires born from 1962 through 1970 and 2) 1980s sires born in 1980 through 1987. The mixed model included cow age, season of measurement and their interactions, year of birth, pregnancy-lactation code (PL), and breedgroup as fixed effects for CW and CS. Analyses of weight adjusted for condition score included CS as a linear covariate. The model for CH excluded PL. Random effects were additive genetic and permanent environmental effects associated with the cow. Differences among breed groups were significant (P < 0.05) for all traits and were maintained through maturity with few interchanges in ranking. The order of F1 cows for weight was as follows: Charolais (506 to 635 kg for different ages), Shorthorn and Salers, reciprocal Hereford-Angus (HA) with 1980s sires, Nellore, HA with reference sires, Galloway, Piedmontese, and Longhorn (412 to 525 kg for different ages). Order for height was as follows: Nellore (136 to 140 cm), Charolais, Shorthorn, Salers, HA with 1980s sires, Piedmontese, Longhorn, Galloway and HA with reference sires (126 to 128 cm). Hereford and Angus cows with reference sires were generally lighter than those with 1980s sires. In general, breed differences for height followed those for weight except that F1 Nellore cows were tallest, which may in part be due to Bos taurus-Bos indicus heterosis for size.     

液相色谱-串联质谱法测定动物尿液中11种β-受体激动剂残留量的不确定度评估

采用液相色谱-串联质谱法测定动物尿液中11种β-受体激动剂残留量,对标准溶液、体积、质谱峰面积、浓缩过程及回收率等测定不确定度因素进行了分析,通过评定各不确定度分量及标准不确定度,得出11种β-受体激动剂的扩展不确定度在0.7 ～ 1.1 ng/mL范围内.由各因素对合成不确定度的贡献比分析可知,影响较大的因素为试验回收率及标准溶液浓度.     

Granulocyte isolation from whole blood of goat, sheep, cattle, horse, dog, pig, and man

A simple two step procedure for the isolation of caprine, ovine, bovine, equine, canine, porcine and human peripheral blood granulocytes is described. After enrichment of granulocytes by centrifugation, contaminating erythrocytes are lysed hypotonically. Recovery, purity, and viability of the granulocyte suspensions are determined. FACScan analysis of the cell suspensions measuring cellular size by forward and sideward light scatter is compared with the corresponding analysis of whole blood leukocytes. Constituencies of the isolated cell suspensions and loss of granulocyte subpopulations through isolation procedure is discussed with regard to granulocyte function assays.