Occurrence of tan spot of wheat caused by Pyrenophora tritici-repentis on wheat in England and Wales in 1987

Drechslera tritici-repentis , anamorph of Pyrenophora tritici-repentis , was found in diamond-shaped, dark-brown lesions on leaves of several winter wheat crops in England and Wales. Lesions were darker than the otherwise similar lesions caused by Septoria nodorum.     

Dynamics of primary and secondary infection in take-all epidemics

ABSTRACT Using a combination of experimentation and mathematical modeling, the effects of initial (particulate) inoculum density on the dynamics of disease resulting from primary and secondary infection of wheat by the take-all fungus, Gaeumannomyces graminis var. tritici, were tested. A relatively high inoculum density generated a disease progress curve that rose monotonically toward an asymptote. Reducing the initial inoculum density resulted in a curve that initially was monotonic, rising to a plateau, but which increased sigmoidally to an asymptotic level of disease thereafter. Changes in the infectivity of particulate inoculum over time were examined in a separate experiment. Using a model that incorporated terms for primary and secondary infection, inoculum decay, and host growth, we showed that both disease progress curves were consistent with consecutive phases dominated, respectively, by primary and secondary infection. We examined the spread of disease from a low particulate inoculum density on seminal and adventitious root systems separately. Although seminal roots were affected by consecutive phases of primary and secondary infection, adventitious roots were affected only by secondary infection. We showed that the characteristic features of disease progress in controlled experiments were consistent with field data from crops of winter wheat. We concluded that there is an initial phase of primary infection by G. graminis var. tritici on winter wheat as seminal roots grow through the soil and encounter inoculum, but the rate of primary infection slows progressively as inoculum decays. After the initial phase, there is an acceleration in the rate of secondary infection on both seminal and adventitious roots that is stimulated by the increase in the availability of infected tissue as a source of inoculum and the availability of susceptible tissue for infection.     

Deposition of spray liquid on the soil below cereal crops after applications during the growing season

The aim of this study was to investigate how big a proportion of the spray liquid applied in a typical application was deposited on the soil surface below the crop. From an agronomical point of view this part of the spray is an undesirable loss of foliar acting pesticides during application and it also constitutes a primary source of leaching and surface run‐off. The investigation was carried out during 1998–2001 in winter wheat and in spring barley. Applications were carried out at approximate intervals of 10 days during the whole growing season in the two cereal crops. The results showed that a high proportion of the spray liquid reaches the soil below the crop during applications at early growth stages corresponding to the growth stages where weed control on farms is carried out. At later growth stages soil deposition declined and reached values below 5% of the applied dose in winter wheat after inflorescence emergence (50–59 BBCH). The spring barley crops remained more open and minimum soil deposit values were approximately 15% of the applied dose at growth stages (50–59 BBCH). During ripening soil deposition values increased again as crop cover decreased.     

东北春麦区小麦白粉菌的侵染循环

 小麦白粉菌(Blumeria graminis DC.Speer)可以在我国东北的南部冬麦区以菌丝垫形态潜伏在小麦苗基部叶片和叶鞘上越冬,以混在干燥保存的小麦种子中的闭囊壳越夏。自生麦苗和白粉病的野生寄主在侵染循环中不起作用。小麦白粉菌的毒力频率(Vf)分析表明,公主岭、沈阳、海城、大连和烟台的小麦白粉菌同属于一个群体。东北春麦区小麦白粉病初发日期和高空天气图分析表明,东北春麦区小麦白粉病的初侵染菌源来自胶东半岛冬麦区。在一定的天气系统控制下,小麦白粉菌的分生孢子随夏初的偏南气流,在700毫巴高度层向北传播,进入东北春麦区。随降雨沉降,并侵染小麦植株。     

Effect of tillage, cover crop and crop rotation on the composition of weed flora in a sandy soil

The development of integrated weed management strategies requires knowledge of mechanisms that influence compositional changes in weed flora. A 9-year study was initiated in 1988 at Delhi, Canada, on a loamy sand soil to evaluate the effect of tillage systems [conventional (CT) and no-till (NT)] and cover crops (only in NT) on weed density, species composition and associations, and crop yield in a winter wheat ( Triticum aestivum L.)/bean/winter wheat rotation. Three bean types: soyabean ( Glycine max L. Merr.), white bean ( Phaseolus vulgaris L.) and kidney bean ( P . vulgaris L.) were included. The NT system included variations: rye ( Secale cereale L.) or maize ( Zea mays L.) cover crop, volunteer wheat disked after harvest and wheat stubble. Data were collected in 1994, 1995 and 1996. Tillage systems, cover crops and crop type had differential effects on weed densities, species composition and associations. Weed densities were not affected by tillage or cover crops in wheat but, in the beans, densities were greater in the CT than in the NT systems. Various associations of weed species with tillage system, cover crop and crop type were observed. Crop yields were not affected by tillage type or cover crop, except that soyabean yields were highest in plots with cover crops.     

Long-term weed community dynamics in Swiss organic and integrated farming systems

In 1991, a farming-system comparison was established on Burgrain Farm (Alberswil) to investigate the long-term sustainability of farming systems in Switzerland. In this study, the impacts of the three farming systems [organic (ORG), and integrated (IF) with an extensive (IF ext ) and an intensive (IF int ) variant] on weed dynamics and diversity in six fields planted with winter wheat, maize, summer/winter barley, potatoes/oilseed rape and temporary grassland are examined. Altogether, 51 plant species were recorded from 1999 to 2006 in the maize and winter wheat crop. Total weed ground cover prior to harvest was seven times higher for wheat and 15 times higher for maize in ORG than in IF int , but grain yields were not negatively affected. Weed diversity was higher for ORG than for IF. In the temporary grassland, Taraxacum officinale and Rumex obtusifolius increased with time and dominated the weed community in the maize which followed. Chenopodium and Polygonum species dominated in the wheat, especially in ORG. We conclude from this study that an optimal combination of direct and indirect means for controlling weeds would allow organic farming at this site, provided that problematic weeds (e.g. Elytrigia repens ) can be kept at the low level observed at the end of 2006.     

Thrips (Thysanoptera: Thripidae) mitigation in seedling cotton using strip tillage and winter cover crops

BACKGROUND: Thrips are the most consistent insect pests of seedling cotton in the southeastern United States, where symptoms can range from leaf curling to stand loss. In a 2 year study, thrips adults and immatures were sampled at 14, 21 and 28 days after planting on cotton planted with a thiamethoxam seed treatment in concert with crimson clover, wheat or rye winter cover crops and conventional or strip tillage to investigate potential differences in thrips infestations. RESULTS: Densities of adult thrips, primarily Frankliniella fusca (Hinds), peaked on the first sampling date, whereas immature densities peaked on the second sampling date. Regardless of winter cover crop, plots that received strip tillage experienced significantly fewer thrips at each sampling interval. In addition, assessment of percentage ground cover 42 days after planting showed that there was more than twice as much ground cover in the strip‐tilled plots compared with conventionally tilled plots. Correlation analyses showed that increased ground cover was inversely related to thrips densities that occurred on all three sampling dates in 2008 and the final sampling date in 2009. CONCLUSIONS: Growers who utilize strip tillage and a winter cover crop can utilize seed treatments for mitigation of early‐season thrips infestation. Copyright © 2010 Society of Chemical Industry     

Reaction of selected winter wheat cultivars from Europe and United States to Karnal bunt

A study was conducted to determine the susceptibility of popular United States (U.S.) and European winter wheat cultivars to the fungal pathogen Tilletia indica. Historically, the disease has been limited to autumn-sown spring-habit wheat areas and not associated with winter wheat. In 1997, Karnal bunt was observed on winter wheat in limited regions of Texas. This region marks the southern end of the contiguous U.S. central winter wheat belt, which extends north into Canada. The aim of this study was to assess the levels of disease resistance in winter wheat. Fifty U.S. and European winter wheat cultivars were tested using two different greenhouse inoculation procedures. For each cultivar, 12 spikes in boot were inoculated by boot-injection with a sporidial suspension (1.0 ml/boot, 10,000 spores ml⁻¹), and 12 other emerged spikes were spray-inoculated with the same concentration. The experiment was repeated for three seasons. Among cultivars, mean seed infection ranged from 2.1 to 87.2% and 0 to 15.6% for boot-injected and spray-inoculated treatments, respectively. Results showed that the majority of winter wheat cultivars tested were susceptible to Karnal bunt.     

An Epidemiological Analysis of the Role of Disease-Induced Root Growth in the Differential Response of Two Cultivars of Winter Wheat to Infection by Gaeumannomyces graminis var. tritici

ABSTRACT Epidemiological modeling combined with parameter estimation of experimental data was used to examine differences in the contribution of disease-induced root production to the spread of take-all on plants of two representative yet contrasting cultivars of winter wheat, Ghengis and Savannah. A mechanistic model, including terms for primary infection, secondary infection, inoculum decay, and intrinsic and disease-induced root growth, was fitted to data describing changes in the numbers of infected and susceptible roots over time at a low or high density of inoculum. Disease progress curves were characterized by consecutive phases of primary and secondary infection. No differences in root growth were detected between cultivars in the absence of disease and root production continued for the duration of the experiment. However, significant differences in disease-induced root production were detected between Savannah and Genghis. In the presence of disease, root production for both cultivars was characterized by stimulation when few roots were infected and inhibition when many roots were infected. At low inoculum density, the transition from stimulation to inhibition occurred when an average of 5.0 and 9.0 roots were infected for Genghis and Savannah, respectively. At high inoculum density, the transition from stimulation to inhibition occurred when an average of 4.5 and 6.7 roots were infected for Genghis and Savannah, respectively. Differences in the rates of primary and secondary infection between Savannah and Genghis also were detected. At a low inoculum density, Genghis was marginally more resistant to secondary infection whereas, at a high density of inoculum, Savannah was marginally more resistant to primary infection. The combined effects of differences in disease-induced root growth and differences in the rates of primary and secondary infection meant that the period of stimulated root production was extended by 7 and 15 days for Savannah at a low and high inoculum density, respectively. The contribution of this form of epidemiological modeling to the better management of take-all is discussed.     

Pseudocercosporella anguioides, a weakly pathogenic fungus associated with eyespot in winter wheat at a site in England

Pseudocercosporella anguioides is reported for the first time from Britain. Isolates were made from the leaf sheaths of four plants of a sample of 2716 with suspected eyespot lesions taken in April 1986 from a winter wheat crop. The eyespot pathogen, P. herpotrichoides , was isolated from 724 plants in the same sample. P. anguioides was not found in a sample of eyespot-infected straws taken from the same crop in July 1986. In infection tests, the P. anguioides isolates produced no obvious lesions on the leaf sheaths of wheat seedlings grown in pots, but were sometimes reisolated from symptomless leaf sheaths. Although P. anguioides occurred infrequently, care is needed to distinguish it from P. herpotrichoides when monitoring the eyespot pathogen.     

新疆拜城冬小麦地主要杂草类型与不同轮作方式杂草种类调查

采用对角线5点取样法调查新疆拜城地区冬小麦田杂草发生情况,结果表明：该地区冬小麦地有杂草16科41种,一年生杂草占60．98％,多年生杂草占39．02％,优势杂草为无芒稗、狗尾草、藜,其密度分别为120．7、32．6、14．1株／m^2;冬小麦—青贮玉米、冬小麦—草木樨能够抑制冬小麦地杂草的发生,其中冬小麦套种草木樨抑制杂草效果极显著（P〈0．01）。     

甘肃省冬小麦干旱灾害风险评估及其区划

为提高西北旱作冬小麦干旱风险管理水平,选用甘肃省41个气象站1971—2016年逐日常规气象观测资料及甘肃省冬小麦农业生产相关资料,基于自然灾害风险理论,从危险性、暴露性、脆弱性、防灾能力4个因子出发,建立了甘肃省冬小麦干旱灾害风险评估模型,并用Arc GIS对甘肃省冬小麦进行干旱风险区划。结果表明:甘肃省冬小麦全生育期干旱高危险区和次高危险区主要位于陇中北部、陇东北部和陇南南部;高暴露区和次高暴露区集中在陇东和陇南地区;高脆弱区和次高脆弱区主要位于陇东大部;次低防灾能力区和低防灾能力区位于陇东大部和陇南北部。甘肃省冬小麦高风险区分布于庆阳市北部和陇南市南部,次高风险区和中度风险区主要位于陇东大部、陇中北部和天水市北部,省内其余冬小麦区属于次低和低风险区。该研究成果将为甘肃冬小麦防灾减灾及可持续发展提供一定理论依据。     

Susceptibility of European bread and durum wheat cultivars to Tilletia indica

Representative European wheat cultivars were tested under quarantine containment for their susceptibility to Tilletia indica, the cause of Karnal bunt of wheat. Fifteen winter and 15 spring wheat ( Triticum aestivum ) and 11 durum wheat ( Triticum durum ) cultivars were inoculated by boot injection just prior to ear emergence to test their physiological susceptibility. Selected cultivars were then re-tested by spray inoculation after ear emergence to determine their morphological susceptibility, which is a better predictor of field susceptibility. At maturity, the ears and seeds were assessed for incidence and severity of disease. For the physiological susceptibility tests, 13/15 winter wheat cultivars were infected and the percentage of infected seeds ranged from 1 to 32%. For spring cultivars, 13/15 cultivars were infected and the percentage of infected seeds ranged from 1 to 48%. For the durum cultivars, 9/11 were infected and the percentage of infected seeds ranged from 2 to 95%. Across all cultivars, 35/41 were infected. Based on historical Karnal bunt susceptibility categories using coefficients of infection, one cultivar was classed as highly susceptible, three as susceptible, 11 as moderately susceptible, 20 as resistant and only six as highly resistant. The spray-inoculation morphological susceptibility tests broadly confirmed the physiological susceptibility results, although lower levels of infection were observed. Overall, the range of susceptibility was similar to that found in cultivars grown in Karnal bunt affected countries. The results demonstrate that European wheat cultivars are susceptible to T. indica and thus could potentially support the establishment of T. indica if introduced into Europe.     

Association study of resistance to Soilborne wheat mosaic virus in U.S. winter wheat

Soilborne wheat mosaic virus (SBWMV) is one of the most important winter wheat pathogens worldwide. To identify genes for resistance to the virus in U.S. winter wheat, association study was conducted using a selected panel of 205 elite experimental lines and cultivars from U.S. hard and soft winter wheat breeding programs. Virus symptoms were evaluated twice in virus-infected fields for the panel at Manhattan, KS in spring 2010 and 2011 and for a subpanel of 137 hard winter wheat accessions at Stillwater, OK in spring 2008. At the two locations, 69.8 and 79.5% of cultivars were resistant or moderately resistant to the disease, respectively. After 282 simple-sequence repeat markers covering all wheat chromosome arms were scanned for association in the panel, marker Xgwm469 on the long arm of chromosome 5D (5DL) showed a significant association with the disease rating. Three alleles (Xgwm469-165bp, -167bp, and -169bp) were associated with resistance and the null allele was associated with susceptibility. Correlations between the marker and the disease rating were highly significant (0.80 in Manhattan at P < 0.0001 and 0.63 in Stillwater at P < 0.0001). The alleles Xgwm469-165bp and Xgwm469-169bp were present mainly in the hard winter wheat group, whereas allele Xgwm469-167bp was predominant in the soft winter wheat. The 169 bp allele can be traced back to 'Newton', and the 165 bp allele to Aegilops tauschii. In addition, a novel locus on the short arm of chromosome 4D (4DS) was also identified to associate with the disease rating. Marker Xgwm469-5DL is closely linked to SBWMV resistance and highly polymorphic across the winter wheat accessions sampled in the study and, thus, should be useful in marker-assisted selection in U.S. winter wheat.     

我国小麦秋苗条锈病发生规律及其区间菌源传播关系

全国冬小麦秋苗均可遭受来自西北和西南越夏区条锈菌的侵染与危害, 条锈菌在寄主上进一步繁殖和发展, 引起本地或者外地小麦条锈病流行。不同麦区秋苗条锈病发生时间、发生程度及其菌源传播规律各不相同, 年度间亦存在差异。根据小麦条锈病发生流行频率、病菌越冬和越夏情况、秋季菌源和春季菌源的有无与多少、提供时间及其影响范围与作用, 结合地理生态条件、气候特点、小麦种植区划与栽培模式等, 将中国小麦条锈病发生流行区域划分为8个明显不同的生态区系, 即关中、华北春季流行区; 成都平原、江汉流域冬季繁殖区; 西北、川西北越夏易变区; 云贵高原越夏冬繁区; 新疆冬春麦常发区; 西藏高原青稞、小麦常发区; 南方晚播冬麦偶发区; 内蒙古、东北春麦偶发区。通过病害实地调查、病菌群体遗传多样性和高空气流轨迹分析, 进一步揭示了区间菌源传播关系。陇南、陇中、陇东、宁南、海东、陕西宝鸡以及川西北和云贵高原等地区离越夏区较近, 冬小麦播种较早, 秋苗条锈病发生早、发病重, 秋季随西北气流传播到平原冬麦区和海拔较低的冬麦区侵染危害秋播麦苗, 其菌源数量对全国小麦条锈病发生流行程度起着至关重要的作用, 是中国小麦条锈病的秋季菌源基地, 面积约67万hm2; 成都平原、江汉流域、陕南、豫南、云贵坝区等麦区, 离条锈菌越夏区相对较远, 小麦播种期也较晚, 秋苗发病较晚较轻, 但冬季气候温和, 雨露条件充沛, 条锈菌在冬季可以不断侵染和繁殖, 在早春可积累大量菌源, 然后向北部和西部广大麦区扩散传播, 引起小麦条锈菌春季侵染, 是中国小麦条锈病的春季菌源基地, 面积约200万hm2。云贵高原越夏冬繁区可为我国广大麦区特别是长江中下游麦区提供部分菌源, 荆州等鄂东南地区是云贵菌源向长江下游麦区传播的中转站。云南与甘肃之间存在大量的基因流, 推测云南可能是中国小麦条锈菌重要的起源中心。西南和西北秋季菌源对长江流域麦区的相对重要性有待进一步研究明确。     

湖北省小麦条锈菌越夏的初步研究

 调查试验结果表明,在湖北省西北部包括郧阳和神农架两地区,在海拨1700-2050公尺的高山地带,七、八月份最热一旬平均气温,18.7~19.7℃,虽条锈菌可越夏,但很困难.广泛调查残余的绿色小麦、黑麦植株和自生麦苗都没有发现条锈病,不能为本省广大冬麦秋苗提供越夏菌源。调查了十多种禾本科杂草,其中仅在甜茅(Glyceria sp.)上发现很少条锈病夏孢子堆,室内接种可侵染小麦,但在条锈病流行中没有发现其作用.     

Scanning electron microscopy study of infection structure formation of Puccinia graminis f.sp. tritici in host and non-host cereal species

The development of uredospore-derived infection structures of Puccinia graminis f.sp. tritici in wheat, barley, sorghum and maize was examined by scanning electron microscopy (SEM). Germ tubes grew over the leaf surface until a stoma was located. An appressorium formed over the stoma and the leaf was penetrated by an infection peg. Within the substomatal chamber of all species the infection peg developed a substomatal vesicle by 6 h post-inoculation (hpi). from which a primary infection hypha developed parallel to the long axis of the leaf. In wheat, barley and maize, when a primary infection hypha abutted onto a host cell, a septum was laid down between the tip of the hypha and the substomatal vesicle, delimiting a haustorial mother cell by 12 hpi; haustorial mother cells did not form in sorghum. Secondary infection hyphae arose on the substomatal vesicle side of the septum; infection did not progress further in maize, but in wheat and barley secondary infection hyphae branched, and proliferated intercellularly forming the fungal thallus. A haustorial mother cell was delimited when an intercellular hypha abutted onto a host cell. Infection sites with haustorial mother cells were observed at 12 hpi in barley and 24 hpi in wheat. In all four plant species, some atypical substomatal vesicle initials, substomatal vesicles and primary infection hyphae were observed.     

关于旅大地区小麦秆锈菌和叶锈菌夏孢子世代的越冬问题

 关于小麦秆锈和叶锈的越冬问题,国外业巳有许多报告^{[10,13,14,15,16,17,18]}。国内在小麦锈菌越冬方面也有一些报导。     

阿坝州小麦条锈病发生规律的研究

1979—1985年在四川省阿坝州越夏区进行条锈病发生规律调查。该地区海拔2900米以上的晚熟小麦约2.5万亩,条锈病持续发生至9月下旬,为自生麦苗和部分早播秋苗提供菌源。自生麦苗的发病面积近3万亩,其中休闲地约2.5万亩,主要分布在松潘、黑水2400—2900米地区,自生麦苗较多,发病较重;荞麦复种地约4000亩,主要分布在黑水、茂汶和汶川等地1700—2500米地带,自生麦苗较少,发病较轻。自生麦苗提供菌源的关键时期是9月上旬至10月下旬。秋苗发病面积约3.7万亩,主要分布在松潘、黑水2700—2950米地带,自9月中旬至12月下旬不断发生流行,而以8月中旬至9月中旬播种的秋苗发病较重,菌源较多,这在越夏菌源传播中起着很重要的作用。条锈菌在阿坝州又可越冬,构成周年侵染循环,常年流行,这不仅严重危害当地小麦生产,而且向广大冬麦区秋苗提供大量菌源。因此,综合治理阿坝州越夏区至关重要。     

Epidemiology and chemical control of take-all on seminal and adventitious roots of wheat

ABSTRACT Epidemiological modeling is used to examine the effect of silthiofam seed treatment on field epidemics of take-all in winter wheat. A simple compartmental model, including terms for primary infection, secondary infection, root production, and decay of inoculum, was fitted to data describing change in the number of diseased and susceptible roots per plant over thermal time obtained from replicated field trials. This produced a composite curve describing change in the proportion of diseased roots over time that increased monotonically to an initial plateau and then increased exponentially thereafter. The shape of this curve was consistent with consecutive phases of primary and secondary infection. The seed treatment reduced the proportion of diseased roots throughout both phases of the epidemic. However, analysis with the model detected a significant reduction in the rate of primary, but not secondary, infection. The potential for silthiofam to affect secondary infection from diseased seminal or adventitious roots was examined in further detail by extending the compartmental model and fitting to change in the number of diseased and susceptible seminal or adventitious roots. Rates of secondary infection from either source of infected roots were not affected. Seed treatment controlled primary infection of seminal roots from particulate inoculum but not secondary infection from either seminal or adventitious roots. The reduction in disease for silthiofam-treated plants observed following the secondary infection phase of the epidemic was not due to long-term activity of the chemical but to the manifestation of disease control early in the epidemic.