共查询到20条相似文献,搜索用时 31 毫秒
1.
Effects of the Consumption of Caffeinated and Decaffeinated Instant Coffee Beverages on Oxidative Stress Induced by Strenuous Exercise in Rats 总被引:1,自引:1,他引:0
Viana AL Fonseca Md Meireles EL Duarte SM Rodrigues MR Paula FB 《Plant foods for human nutrition (Dordrecht, Netherlands)》2012,67(1):82-87
Many authors attribute the antioxidant activity of brewed coffee to its caffeine content. In addition, caffeine intake has
been associated with increased performance during physical exercise. This study analyzed the in vivo effects of drinking caffeinated and decaffeinated instant coffee (8%, w/v) on oxidative stress and antioxidant enzyme activity
in the anterior tibialis muscles of rats subjected to intense exercise. It was observed that exercise induced lipid peroxidation
(estimated using malondialdehyde) and protein oxidation (evaluated by determining the formation of carbonyl groups) in the
muscle (P < 0.05). Decaffeinated instant coffee and caffeine solution did not exhibit antioxidant activity in vivo. Caffeinated instant coffee beverage intake did not induce changes in superoxide dismutase and glutathione peroxidase activities
but was able to diminish lipid and protein oxidation in the anterior tibialis muscles of rats after exercise (P < 0.05), contributing to a reduction in the oxidative stress triggered by exercise. 相似文献
2.
Jimenez M Guzman AP Azuara E Garcia O Mendoza MR Beristain CI 《Plant foods for human nutrition (Dordrecht, Netherlands)》2012,67(1):57-63
Porophyllum tagetoides is an annual warm-weather herb that has an intense typical smell. Its leaves are commonly used in soup preparation and traditional
medicine for treatment of inflammatory diseases. Its volatile compounds and antioxidant properties were evaluated in crude,
aqueous and ethanol leaf extract and an oil emulsion using different antioxidant assays in vitro, such as: DPPH radical scavenging activity, redox potential, polyphenol content, reducing power and optical density. A high
antioxidative activity was found when comparing leaves with stems. The crude extract from leaves showed a very high reducing
power (2.88 ± 0.20 O.D.) and DPPH radical-scavenging activity (54.63 ± 4.80%), in concordance with a major concentration of
vitamin C (23.97 ± 0.36 mg/100 g). Instead, the highest polyphenol content (264.54 ± 2.17 mg GAE/g of sample) and redox potential
(561.23 ± 0.15 mV) were found by the ethanol and aqueous extract, respectively. Aldehydes and terpenes such as nonanal, decanal,
trans-pineno, β-myrcene and D-limonene were the major volatiles found. This study suggests that Porophyllum tagetoides extracts could be used as antioxidants. 相似文献
3.
Ban X Huang B He J Chen Y Zeng H Han L Wang Y 《Plant foods for human nutrition (Dordrecht, Netherlands)》2011,66(2):175-180
The inflorescence of cultivated Coptis chinensis has been valued for tea production for many years in China. The antioxidant activities of C. chinensis inflorescence extracts prepared by various solvents were investigated by using several established in vitro systems: 2,2′-azinobis(3-ethylbenzthiazoline-6-sulphonic acid (ABTS), α,α-diphenyl-β-picrylhydrazyl (DPPH) and superoxide
radical scavenging assays, reducing power assay, and ferrothiocyanate (FTC) and thiobarbituric acid (TBA) assays. The results
showed that the 70% ethanol extract (EE) had the strongest antioxidant activity in vitro among the various extracts. Based on the in vitro results, EE was used to evaluate the antioxidant activity of C. chinensis inflorescence in vivo. The liver and kidney of intoxicated animals showed a significant decrease in superoxide dismutase (SOD), catalase (CAT)
and glutathione (GSH) levels, while the malondialdehyde (MDA) level showed a significant increase. These changes were significantly
reversed after treatment with EE and the standard vitamin E. Thus, the C. chinensis inflorescence may be a valuable natural source that can be applicable to food industries. 相似文献
4.
Pahua-Ramos ME Ortiz-Moreno A Chamorro-Cevallos G Hernández-Navarro MD Garduño-Siciliano L Necoechea-Mondragón H Hernández-Ortega M 《Plant foods for human nutrition (Dordrecht, Netherlands)》2012,67(1):10-16
Avocado seed contains elevated levels of phenolic compounds and exhibits antioxidant properties. We investigated the effect
of Avocado Seed Flour (ASF) on the lipid levels in mice on a hyperlipidemic diet. The concentration of phenols was determined
by high-performance liquid chromatography, antioxidant activity was evaluated using the Trolox equivalent antioxidant capacity
method, and dietary fiber was measured using the Association of Official Analytical Chemists (AOAC) method. The LD50 of ASF was determined using Lorke’s method and hypolipidemic activity was evaluated in a hypercholesterolemic model in mice.
Protocatechuic acid was the main phenolic compound found in ASF, followed by kaempferide and vanillic acid. The total phenolic
content in the methanolic extract of ASF was 292.00 ± 9.81 mg gallic acid equivalents/g seed dry weight and the antioxidant
activity resulted in 173.3 μmol Trolox equivalents/g DW. In addition, a high content of dietary fiber was found (34.8%). The
oral LD50 for ASF was 1767 mg/kg body weight, and treatment with ASF significantly reduced the levels of total cholesterol, LDL-C,
and prediction of the atherogenic index. Therefore, the antioxidant activity of phenolic compounds and dietary fiber in ASF
may be responsible for the hypocholesterolemic activity of ASF in a hyperlipidemic model of mice. 相似文献
5.
Viviana R. Lucero López Gabriela S. Razzeto Nora L. Escudero M. Sofía Gimenez 《Plant foods for human nutrition (Dordrecht, Netherlands)》2013,68(4):396-402
Hyperlipidemia and hepatic steatosis are frequent alterations due to alcohol abuse. Amaranth is a pseudocereal with hypolipidemic potential among other nutraceutical actions. Here we study the effect of Amaranthus hypochondriacus (Ah) seeds on serum and liver lipids, and the expression of genes associated to lipid metabolism and liver histology in male Wistar rats intoxicated with ethanol. The animals were divided into four groups; two groups were fed the American Institute of Nutrition 1993 for maintenance diet (AIN-93M), and the other two with AIN-93M containing Ah as protein source. One of each protein group received 20 % ethanol in the drinking water, thus obtaining: CC (control casein), EC (ethanol casein), CAh (control Ah) and EAh (ethanol Ah). When comparing EAh vs. EC, we found a positive effect of Ah on lipids, preventing the increment of serum cholesterol (p?<?0.001), through the higher expression of the LDL receptor (p?<?0.001); and it also decreased free (p?<?0.05) and esterified cholesterol (p?<?0.01) in liver, probably via the reduction of the 3-hydroxy-3-methylglutaryl coenzyme A reductase expression (p?<?0.001). We also observed that amaranth contributed to the decrease of fat deposits in liver, probably through the decrease in acetyl-CoA carboxylase alpha (p?<?0.01), glycerol-3-phosphate acyltransferase 1 (p?<?0.01) and diacylglycerol O-acyltransferase 2 (p?<?0.05) expression. The histological study showed a decrease in the fat deposits in the amaranth group when compared to casein; this is consistent with the biochemical and molecular parameters studied in this work. In conclusion, amaranth could be recommended to avoid the alterations in the lipid metabolism induced by alcohol and other harmful agents. 相似文献
6.
Adriana Schultz Moreira Laura González-Torres Raul Olivero-David Sara Bastida Juana Benedi Francisco J. Sánchez-Muniz 《Plant foods for human nutrition (Dordrecht, Netherlands)》2010,65(3):290-298
The effects of diets including restructured meats (RM) containing Wakame or Nori on total liver glutathione status, and several
antioxidant enzyme gene expressions and activities were tested. Six groups of ten male growing Wistar rats each were fed a
mix of 85% AIN-93 M diet and 15% freeze-dried RM for 35 days. The control group (C) consumed control RM, the Wakame (W) and
the Nori (N) groups, RM with 5% Wakame and 5% Nori, respectively. Animals on added cholesterol diets (CC, CW, and CN) consumed
their corresponding basal diets added with cholesterol (2%) and cholic acid (0.4%). Alga and dietary cholesterol significantly
interact (P < 0.002) influencing all enzyme expressions but not activities. The cholesterol supplement decreased most enzyme expression
and activity. W-RM vs. C-RM increased (P < 0.05) expression of GPx, GR, Mn-SOD, and Cu,Zn-SOD and decreased that of catalase. N-RM vs. C-RM increased (P < 0.05) expression of catalase and Mn-SOD. GR activity increased in W-RM rats while SOD activity increased, but that of Se-GPx
decreased in N animals. W-RM increased total and reduced glutathione and decreased the redox index. CN diet induced significantly
lower plasma cholesterol levels (P < 0.001) than the CW diet. In conclusion, Nori-RM is a hypocholesterolemic food while Wakame-RM is an antioxidant food. This
should be taken into account when including this kind of RM as potential functional foods in human. 相似文献
7.
Doolaege EH Raes K De Vos F Verhé R De Smet S 《Plant foods for human nutrition (Dordrecht, Netherlands)》2011,66(2):196-202
The absorption, distribution and elimination of carnosic acid, the main antioxidant found in rosemary was studied, in vivo, in rats. Therefore, carnosic acid was administrated in a single dose, intravenously (20.5 ± 4.2 mg/kg) and orally (64.3 ± 5.8 mg/kg),
to four and nine rats, respectively. Blood samples were collected at different time points, and plasma concentrations of carnosic
acid were determined using LC-MS. Furthermore, total collection of urine and feces was done during 4 h and 24 h for the intravenous
and oral administrations, respectively. After euthanizing the rats, intestinal content, liver and muscle tissue were sampled
to determine carnosic acid concentrations. The bioavailability of carnosic acid, after 360 min, was 40.1%. Traces of carnosic
acid were found in the rats intestinal content, liver and muscle tissue of abdomen and legs. The recovery of carnosic acid
in the feces, 24 h after oral administration, was 15.6 ± 8.2%. Carnosic acid is absorbed into the bloodstream after oral administration
in rats and is therefore bioavailable. It was found that carnosic acid in vivo is present in its free form and that its main elimination route is the fecal route. 相似文献
8.
Antioxidant activity of pressurized low polarity water (PLPW) extracts of cow cockle seed and extraction residues were determined
using DPPH, ABTS, and FRAP assays. The effect of extraction conditions (temperature (125, 150 and 175 °C) and time) on the
antioxidant activity and the relationship amongst the antioxidant activity and extract composition (total phenolics and saponin
content) were determined. The antioxidant activity of PLPW extracts increased with extraction temperature. Increasing activity
with time was also observed at 175 °C. PLPW extraction residues had the highest activity suggesting antioxidant compounds
were not completely extracted by PLPW. Antioxidant activity correlated well with total phenolics content of samples (R
2 ≥ 0.94), however no correlation was observed with the saponin content. A strong correlation was observed between the antioxidant
activity values obtained using different methods (R
2 ≥ 0.94). These results point to the potential of PLPW extraction as a method to modify the activity of biological materials
for the production of customized extracts. 相似文献
9.
Yun S Zhang T Li M Chen B Zhao G 《Plant foods for human nutrition (Dordrecht, Netherlands)》2011,66(3):212-217
The objective of this study was to evaluate the effect of proanthocyanidins (PAs) on iron uptake from soybean seed ferritin
(SSF) crude by rats with iron deficiency anemia (IDA) for the first time. Six groups of Sprague–Dawley (SD) rats (n = 10) were used, which contain (1) SSF crude group; (2) SSF crude + PAs group; (3) PAs group; (4) FeSO4 group; (5) iron deficiency control group; and (6) control group. The bioavailability of iron was examined by measuring hemoglobin
(Hb) concentration value, red blood cell (RBC) numbers, and serum iron stores. After 8 weeks, Hb concentration was almost
recovered to the normal level upon feeding SSF crude or FeSO4 to rats. In contrast, Hb concentration was recovered to less extent when SSF crude plus PAs was used instead of SSF crude
alone (P < 0.05). A similar profile was observed with these three sample groups when serum iron and RBC were used as parameters. All
rats in PAs group died at the 8th week. Taken together, all these results demonstrated that PAs inhibited iron uptake of rats
from SSF, and are toxic for rats with IDA. 相似文献
10.
Tundis R Loizzo MR Menichini F Bonesi M Conforti F Statti G De Luca D de Cindio B Menichini F 《Plant foods for human nutrition (Dordrecht, Netherlands)》2011,66(3):261-269
The present study aimed to evaluate for the first time the phenols, flavonoids, carotenoids, capsaicin and dihydrocapsaicin
content and the antioxidant and hypoglycemic properties of Capsicum annuum var. acuminatum small and C. annuum var. cerasiferum air-dried fruits. The ethanol extract of C. annuum var. acuminatum small, characterized by the major content of total poliphenols, flavonoids, carotenoids and capsaicinoids, showed the highest
radical scavenging activity (IC50 of 152.9 μg/ml). On the contrary, C. annuum var. cerasiferum showed a significant antioxidant activity evaluated by the β-carotene bleaching test (IC50 of 3.1 μg/ml). The lipophilic fraction of both C. annuum var. acuminatum and C. annuum var. cerasiferum exhibited an interesting and selective inhibitory activity against α-amylase (IC50 of 6.9 and 20.1 μg/ml, respectively). 相似文献
11.
Jarinyaporn Naowaboot Patchareewan Pannangpetch Veerapol Kukongviriyapan Bunkerd Kongyingyoes Upa kukongviriyapan 《Plant foods for human nutrition (Dordrecht, Netherlands)》2009,64(2):116-121
In Thailand, beverages containing mulberry leaf (Morus alba L.) are believed to promote good health, especially in people with diabetes. We examined the effects of long-term administration
of an ethanolic extract of mulberry leaf (MA) on blood glucose, oxidative damage, and glycation in streptozotocin-induced
diabetic rats. Daily administration of 1 g/kg MA for six weeks decreased blood glucose by 22%, which was comparable to the
effect of 4 U/kg insulin. Lipid peroxidation, measured as malondialdehyde and lipid hydroperoxide concentrations (3.50 ± 0.33
and 3.76 ± 0.18 μM, respectively) decreased significantly (P < 0.05) compared to nontreated control diabetic rats (8.19 ± 0.45 and 7.50 ± 0.46 μM, respectively). Hemoglobin A1C, a biomarker for chronic exposure to high concentration of glucose, was also significantly decreased in the MA-treated group
(6.78 ± 0.30%) in comparison to untreated group (9.02 ± 0.30%). The IC50 of in vitro antiglycation and free radical scavenging activities of MA were 16.4 ± 5.6 μg/ml and 61.7 ± 2.1 μg/ml, respectively. These
findings support that long-term administration of MA has antihyperglycemic, antioxidant and antiglycation effects in chronic
diabetic rats, which may be beneficial as food supplement for diabetics. 相似文献
12.
Rajangam Udayakumar Sampath Kasthurirengan Ayyappan Vasudevan Thankaraj Salammal Mariashibu Jesudass Joseph Sahaya Rayan Chang Won Choi Andy Ganapathi Sei Chang Kim 《Plant foods for human nutrition (Dordrecht, Netherlands)》2010,65(2):91-98
The phenolic compounds and flavonoids were determined from the extracts of Withania somnifera root (WSREt) and leaf (WSLEt). The WSREt has 28.26 mg/g total phenolic compounds and 17.32 mg/g flavonoids, whereas WSLEt
has 5.4 mg/g total phenolic compounds and 5.1 mg/g flavonoids. The WSREt, WSLEt and glibenclamide were orally administered
daily to diabetic rats for 8 weeks. After the treatment, the levels of urine sugar, blood glucose, liver glycogen, and antioxidants
like vitamin C and E in plasma and superoxide dismutase (SOD), catalase (CAT), thiobarbituric acid reactive substances (TBARS),
glutathione peroxidase (GPx), glutathione-S-transferase (GST) and reduced glutathione (GSH) in liver, kidney and heart were
determined. Diabetic rats showed a significant (p < 0.05) elevation in glucose and TBARS and a significant (p < 0.05) reduction in glycogen, vitamin C and E, SOD, CAT, GPx, GST, and GSH levels when compared to normal control rats. Administration
of WSREt, WSLEt and glibenclamide to diabetic rats restored the levels to normal. In the light of aforesaid facts, it is suggested
that the presence of phenolic compounds including flavonoids in W. somnifera root and leaf extracts and their antioxidant activity may play a vital role in reduction of blood glucose level in alloxan-induced
diabetic rats. 相似文献
13.
Korekar G Stobdan T Arora R Yadav A Singh SB 《Plant foods for human nutrition (Dordrecht, Netherlands)》2011,66(4):376-383
Fourteen apricot genotypes grown under similar cultural practices in Trans-Himalayan Ladakh region were studied to find out
the influence of genotype on antioxidant capacity and total phenolic content (TPC) of apricot kernel. The kernels were found
to be rich in TPC ranging from 92.2 to 162.1 mg gallic acid equivalent/100 g. The free radical-scavenging activity in terms
of inhibitory concentration (IC50) ranged from 43.8 to 123.4 mg/ml and ferric reducing antioxidant potential (FRAP) from 154.1 to 243.6 FeSO4.7H2O μg/ml. A variation of 1–1.7 fold in total phenolic content, 1–2.8 fold in IC50 by 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay and 1–1.6 fold in ferric reducing antioxidant potential among the examined
kernels underlines the important role played by genetic background for determining the phenolic content and antioxidant potential
of apricot kernel. A positive significant correlation between TPC and FRAP (r = 0.671) was found. No significant correlation was found between TPC and IC50; FRAP and IC50; TPC and physical properties of kernel. Principal component analysis demonstrated that genotypic effect is more pronounced
towards TPC and total antioxidant capacity (TAC) content in apricot kernel while the contribution of seed and kernel physical
properties are not highly significant. 相似文献
14.
Yoshinori Okada Mizue Okada Yumi Sagesaka 《Plant foods for human nutrition (Dordrecht, Netherlands)》2010,65(3):225-232
To search for dried plant seeds with potent anti-diabetes activity, we conducted a large scale screening for inhibitory activity
on tumor necrosis factor-alpha and facilitating activity on adiponectin production in vitro. These activities in 3T3-L1 adipocytes were screened from ethanol extracts of 20 kinds of dried plant seed marketed in Japan.
komatsuna (Brassica rapa var. perviridis), common bean (Phaseolus vulgaris L.), qing geng cai (Brassica rapa var. chinensis), green soybean (Glycine max), spinach (Spinacia oleracea L.) and sugar snap pea (Pisum sativum L.) markedly enhanced adiponectin production (11.3 ~ 12.7 ng/ml) but Japanese radish (Raphanus sativus), edible burdock (Arctium lappa L.), bitter melon (Momordica charantia) and broccoli (Brassica oleracea var. italica) did not (0.9 ~ 2.7 ng/ml). All adiponectin-production-enhancing seeds except spinach (2.7 pg/ml) and okra (Abelmoschus esculentus) (6.6 pg/ml) effectively decreased tumor necrosis factor-alpha levels (0.0 pg/ml). We further examined the effects on free
radical scavenging activities in the dried seed extracts. Although scavenging activity correlated well with total phenolic
content of samples, no correlation was observed with adiponectin production. These results point to the potential of dried
seed extracts as a means to modify the activity of tumor necrosis factor-alpha for the adiponectin production. 相似文献
15.
Miranda-Velasquez L Oranday-Cardenas A Lozano-Garza H Rivas-Morales C Chamorro-Cevallos G Cruz-Vega DE 《Plant foods for human nutrition (Dordrecht, Netherlands)》2010,65(4):392-395
The aim of this study was to determine the hypocholesterolemic activity of Cnidoscolus chayamansa. In an in vivo model, high-cholesterol diet administered to mice Balb/c induced hypercholesterolemia. Three extracts from Cnidoscolus chayamansa (ethanol, methanol and an aqueous extract) were tested on hypercholesterolemic mice. Active extracts were assessed against
the in vitro inhibitory activity of the same three extracts on the HMG-CoA reductase enzyme by using Vero cells. The specific chemical
groups present in the phytochemical extracts were also determined. Only the aqueous extract (at either doses employed) showed
a significant cholesterol reduction (27.9 and 31.1%, for 50 and 100 mg kg−1, respectively P < 0.01). The extract did not inhibit the HMG-CoA reductase enzyme, suggesting that its compounds act at another level in
cholesterol metabolism. Reactions to secondary metabolites indicate the presence of alkaloids in the aqueous and ethanol extracts
and phenol hydroxyls in the ethanol and methanol extracts. 相似文献
16.
To identify the potential of green leafy vegetables (GLV) as antioxidants, methanolic extracts of Amaranthus sp., Centella asiatica, Murraya koenigii and Trigonella foenum graecum were studied for their antioxidant activity in different systems at multiple concentrations. Total antioxidant activity assessed
by phosphomolybdenum method, free radical scavenging activity by 1,1-diphenly-2-picryl hydrazyl (DPPH), reducing power and
ferrous ion chelating activity were determined. The GLV were analyzed for ascorbic acid, total and β-carotene and total polyphenol
contents. The ascorbic acid, total carotene, β-carotene and total phenolic content (tannic acid equivalents) of the GLV ranged
between 15.18–101.36, 34.78–64.51, 4.23–8.84 and 150.0–387.50 mg/100 g GLV, respectively. The extracts were found to have
significantly different levels of antioxidant activities in the systems tested. The total antioxidant activity was highest
in Murraya koenigii (2,691.78 μmol of ascorbic acid/g sample) and least in Centella asiatica (623.78 μmol of ascorbic acid/g sample). The extract concentration causing 50% inhibition of DPPH (IC50) was determined (M. koenigii < C.asiatica < Amaranthus sp. < T. graecum). The maximum DPPH scavenging activity and reducing power was exhibited by Murraya koenigii. Multiple regression analysis showed that the relationship of total antioxidant activity, free radical scavenging activity,
and reducing power with polyphenol and total and β-carotene was highly significant.
Paper awarded the Young Scientist Award in Experimental Nutrition at the Young Scientist Award Session of the 39th Annual
Meet of the Nutrition Society of India, Hyderabad, India on 15–16th Dec 2007. 相似文献
17.
Paśko P Zagrodzki P Bartoń H Chłopicka J Gorinstein S 《Plant foods for human nutrition (Dordrecht, Netherlands)》2010,65(4):333-338
The effect of Chenopodium quinoa seeds on lipid profile, glucose level, protein metabolism and selected essential elements (Na, K, Ca, Mg) level was determined
in high—fructose fed male Wistar rats. Fructose decreased significantly LDL [42%, p < 0.01] and activity of alkaline phosphatase [20%, p < 0.05], and increased triglycerides level [86%, p < 0.01]. The analysis of blood of rats fed quinoa indicated, that these seeds effectively reduced serum total cholesterol
[26%, p < 0.05], LDL [57%, p < 0.008] and triglycerides [11%, p < 0.05] when compared to the control group. Quinoa seeds also significantly reduced the level of glucose [10%, p < 0.01] and plasma total protein level [16%, p < 0.001]. Fructose significantly decreased HDL [15%, p < 0.05] level in control group but when the quinoa seeds were added into the diet the decrease of HDL level was inhibited.
Quinoa seeds did not prevent any adverse effect of increasing triglyceride level caused by fructose. It was shown in this
study that quinoa seeds can reduce most of the adverse effects exerted by fructose on lipid profile and glucose level. 相似文献
18.
Hua Bai Chunxu Hai Miaomiao Xi Xin Liang Rui Liu 《Plant foods for human nutrition (Dordrecht, Netherlands)》2010,65(3):271-276
Maize silks, dried cut stigmata of maize female flowers, are a traditional medicinal plant. This study was conducted to investigate
the antioxidant effect of maize silks ethanol extract (MSE) against oxidative damage in vivo. γ-radiation was employed to induce oxidative stress in mice and the variation of malondialdehyde (MDA), glutathione/glutathione
disulfide ratio (GSH/GSSG), blood cells, NF-E2-related factor 2 (Nrf2) and related antioxidant enzymes were examined. The
results showed that radiation elevate levels of MDA, induce hematological abnormalities and decrease levels of GSH/GSSG and
Nrf2 expression in liver and kidney. MSE administration significantly abolished elevation of MDA levels in liver, maintained
hepatic GSH/GSSG ratio and ameliorated hematological abnormalities dose dependently. Moreover, MSE up-regulated the hepatic
protein expression of Nrf2 dose dependently and the activities as well as protein expression of Nrf2-related antioxidant enzymes
were also increased. However, the antioxidant ability of MSE seemed not to be as effective in kidney as in liver. These findings
firstly proved the protective role of MSE against oxidative stress, which was in part via up-regulation of Nrf2 and seemed
to be tissue specific. 相似文献
19.
Yong-Seo Park Mi-Kyung Lee Buk-Gu Heo Kyung-Sik Ham Seong-Gook Kang Ja-Yong Cho Shela Gorinstein 《Plant foods for human nutrition (Dordrecht, Netherlands)》2010,65(2):186-191
This study was conducted in order to compare the nutrient and chemical contents of two Korean teas: traditional Chungtaejeon
tea (CTJ) with that of green tea (GT). Main bioactive compounds and the antioxidant activities using four radical scavenging
assays (ABTS, CUPRAC, FRAP and DPPH) in methanol and acetone extracts of both teas were determined. It was found that the
contents of vitamin C, amino acids and total nitrogen in CTJ were lower than that of GT (p < 0.05). Caffeine, reducing sugar and chlorophyll contents in CTJ were similar to GT. Catechin (C), epicatechin (EC), and
epigallocatechin (EGC) contents were lower in CTJ than in green tea (p < 0.05), but gallocatechin (GC), epicatechin gallate (ECG), epigallocatechin gallate (EGCG) and catechin gallate (CG) showed
no significant differences between CTJ and GT (p ≥ 0.05). The contents of polyphenols, flavonoids, flavanols and tannins in
CTJ methanol were 229.30 ± 11.3 mg GAE/g dry weight (DW), 15.24 ± 0.8 mg CE/g DW, 109.10 ± 5.1 mg CE/g DW and 25.68 ± 1.2
mg CE/g DW, respectively, and significantly higher than in acetone extracts (p < 0.05). Flavonoids (quercetin and kaempferol) were higher in GT than in CTJ and myricetin was higher in CTJ (p < 0.05). Threonine and aspartic acid was lower, and glutamic acid was higher in CTJ compared with GT (p < 0.05). Free amino acid content in CTJ and GT showed no significant difference. Potassium and magnesium in CTJ were lower
compared to GT, but no significant difference was found for iron, manganese and calcium. Also, the level of the antioxidant
activity by all four used assays was significantly higher in CTJ and in methanol was higher than in acetone extracts (p < 0.05). In conclusion, traditional fermented Korean tea Chungtaejeon contains high quantities of bioactive compounds and
possesses high antioxidant activity. The contents of the bioactive compounds and the levels of antioxidant activities are
significantly higher in methanol than in acetone extracts. 3-D fluorescence and FTIR- spectroscopy showed slight differences
between the two investigated tea samples and can be used as additional tools for identification of polyphenols. Both studied
teas can be recommended as a source of bioactive compounds. 相似文献
20.
Salvador Gonzalez-Palomares Mirna Estarrón-Espinosa Juan Florencio Gómez-Leyva Isaac Andrade-González 《Plant foods for human nutrition (Dordrecht, Netherlands)》2009,64(1):62-67
The effect of the drying temperature on the volatile components and sensory acceptance of the Roselle (Hibiscus sabdariffa) extract in powder was investigated. The Roselle extraction was carried out by maceration with 7 L of 30% ethanol (v/v), 560 g of fresh Roselle calyces for 168 h. The Roselle extracts were spray dried at different temperatures 150, 160, 170,
180, 190, 200 and 210 °C, giving different outlet values about yield and final moisture. The volatile compounds in Roselle
extract and dried samples were performed using needles of solid phase microextraction (SPME) and gas chromatography/mass spectrometry
(GC/MS HP-5890). Twenty volatile compounds were identified in the extracts among them terpenoids, esters, hydrocarbons and
aldehydes. Fourteen volatile compounds were identified in the powder sample, but only ten were present in the Roselle extract.
This indicates that some compounds were lost and some others were generated due to a degradation process. An acceptability
sensory analysis showed that the best powder sample was the Roselle extract dehydrated using temperature between 190 °C and
200 °C (p < 0.05). There was not statistically significant difference in the pH of Roselle extracts ranging from 3.4 to 3.9. It was
concluded that the spray drying temperature of the Roselle extracts has an effect on the volatile compounds losses. 相似文献