VB12VB1对扁藻繁殖和生长的影响实验

对比实验表明ＶＢ１２ＶＢ１对扁藻的生长无明显影响,不是扁藻生长必需的外源性有机营养,打破了ＶＢ１２ＶＢ１对单胞藻类生长有明显促进作用的传统结论。     

氮源对雨生红球藻和扁藻生长的影响

以缺氮MAV培养基为基本培养基 ,分别添加 30× 10 -6的硝酸钾 (KNO3 )、尿素 ((NH2 ) 2 CO)、硝酸铵 (NH4NO3 )、氯化铵 (CH4Cl)对雨生红球藻和扁藻进行生长试验。结果表明 :不同氮源对雨生红球藻和扁藻的生长均有极显著影响 ,其中NH4Cl最有利于雨生红球藻和扁藻生长 ,NH4NO3 最不利于它们生长     

根瘤菌N_1对扁藻生长影响的研究

研究了由海藻培养体系中分离获得的10株细菌对扁藻生长影响。试验结果表明,菌株N1对自然带菌扁藻及除菌扁藻的生长均有明显的促进作用;经16SrDNA分析表明,菌株N1为根瘤菌属细菌。     

谷氨酸对亚心形扁藻（Platymonas subcordiformis）生长及蛋白质含量的影响

扁藻作为水产动物活饵料，在水产养殖业上具有重要的意义。目前在海产经济动物韵育苗中经常用扁藻作为直接或间接的饵料。为此，如何在短期内培养出优质高产的扁藻是本试验的目的。     

VB1、VB12对盐藻生长繁殖的影响

通过单因子试验和正交试验方法研究了维生素VB1、VB12对盐藻生长繁殖的影响。结果表明:VB1、VB12对盐藻生长繁殖的影响显著,盐藻最适的VB1质量浓度为10 mg/L,VB12质量浓度为0.005 mg/L,其交互作用的优水平为VB120 mg/L,VB120.005 mg/L。     

杜氏盐藻和亚心型扁藻混合培养生长的初步研究

在实验室条件下研究了杜氏盐藻和亚心型扁藻在单独培养和相同接种比例混合培养下的生长情况。结果显示,单独培养盐藻的生长经历了3个明显的阶段,生长曲线呈现"S"型;单独培养扁藻与混合培养藻在18 d内还未到达稳定期,仍保持一定的生长态势。混合培养、单独培养盐藻以及单独培养扁藻的最大光密度值(OD680)分别为0.784、0.702和0.765。混合培养藻的生物量(0.841 mg/ml)也稍高于单独培养盐藻(0.582 mg/ml)和单独培养扁藻的生物量(0.819 mg/ml)。试验结果表明,混合培养盐藻和扁藻具有一定的促进藻生长和提高生物量产出的潜力。     

三十烷醇对亚心形扁藻生长的影响

本文报道了三十烷醇对亚心形扁藻生长的促进作用。其最佳施用浓度为0.1ppm,接种后第4天,藻细胞浓度较对照组提高了51.7%;在扩大培养时,其藻细胞在接种后第12天,较对照组可提高133.4%。     

不同氮源、磷源营养盐对亚心型扁藻(Platymonus subcordiformis)生长的影响

以亚心形扁藻为实验对象,分别以NaNO3、CO(NH2)2和(NH4)2SO4作为氮源,KH2PO4和NaH2PO4作为磷源培养亚心形扁藻,并进行观察和计数,探讨不同氮源和磷源对亚心形扁藻生长的影响.结果显示,培养液中NaNO3、CO(NH2)2、(NH4)25O4、KH2PO4和NaH2PO4都能促进亚心形扁藻的生长,以CO(NH2)2和NaHl2 PO4实验组促生长效果较为明显,亚心形扁藻的生长速率CO(NH2)2(0.0 512)＞(NH4)2SO4(0.0 325)＞NaNO3(0.0 306);在正交实验中,以CO(NH2)2和NaH2PO4为氮、磷源的营养盐组合能够显著促进亚心形扁藻生长,该实验组指数末期藻细胞浓度达到6.1×105cell/mL,是亚心形扁藻作为生物饵料的理想投喂浓度.     

两种抗生素对亚心形扁藻生长的影响

在室温下,研究了不同浓度的土霉素和氯霉素对亚心形扁藻细胞的数量、大小以及叶绿素、类胡萝卜素含量等方面的影响。结果表明:在0~5mg/L范围内,土霉素的浓度越大,细胞数量在增长期内增幅越大,在衰退期内衰退越快;土霉素对色素含量有不同程度的促进作用。氯霉素浓度>1mg/L时,浓度越大,扁藻细胞数量增幅越小,类胡萝卜素越多。土霉素,氯霉素对扁藻细胞大小的影响均不明显。     

氮、磷、铁盐对扁藻生长及组分含量的影响

以海洋微藻培养常用的普适性康维方营养液为基础,按照各营养元素的物质的量分数相等的原则,分别选取不同种类的氮源(硝酸钠、氯化铵、尿素)、铁源(三氯化铁、硫酸亚铁、柠檬酸铁)、磷源(由磷酸二氢钠和磷酸氢二钠组成,同时构成pH分别为7.0、7.5、8.0的缓冲体系),采用L9(34)正交设计试验方案,通过对试验结果的直观分析和方差分析,表明氮源是影响扁藻生长、产物组成及其含量的显著性因素,尿素促进扁藻的生长,并利于蛋白质积累,铵态氮利于淀粉积累;磷酸盐及其所构成的缓冲体系对扁藻总脂积累具有比较显著的影响,初始pH=7.0的磷酸盐缓冲体系利于扁藻油脂的积累。     

4种单胞藻对卤虫生长和生殖影响的比较研究

以亚心形扁藻、小球藻、湛江等鞭金藻、绿色巴夫藻为饵料,卤虫为投喂对象,建立五个实验组分别进行研究,观察不同饵料对卤虫生长和生殖的影响,结果表明:投喂亚心形扁藻的卤虫生长最快,成活率高,生殖量也最多,其次为绿色巴夫藻组,湛江等鞭金藻的饵料效果最差.混合4种单胞藻投喂的效果也较好.     

Effect of vitamin B12 and taurine on the alleviation of nutritional anaemia in common sole (Solea solea)

Sole fed commercial pellets develop a nutritional anaemia. This study assessed the impact of dietary B12 and taurine on the haematocrit (Hct) and haemoglobin (Hb) level and mineral absorption in anaemic sole. Anaemic sole was fed one of four diets. Diets were equal regarding mineral, amino acid and macronutrient composition and formulated, according to a two by two factorial design: two B12 (0.34 vs. 1.9 mg kg dm^?1) and two taurine levels (3.5 vs. 7.6 mg kg dm^?1). The feeding level was restricted and equal for all diets. Hct and Hb levels in anaemic sole are influenced by dietary B12. A “high” level of B12 increases the Hct and Hb level. An increasing level of taurine suppresses the stimulating effect of the “high” level of B12. The applied B12 and taurine levels were unable to completely alleviate the anaemia in sole. Nevertheless, sole needs high dietary levels of B12 to alleviate anaemia. The impact of B12 and taurine on Hb and Hct was not related to a change in the absorption of iron. A “high” level of B12 positively affected the absorption of chromium and a high level of taurine negatively affected the absorption of cobalt in sole.     

初始体重差异对黄颡鱼补偿生长的影响

将黄颡鱼(Pseudobagrus fulvidracoR ichardson)按初始体重不同分为大(L)、小(S)两种规格(平均体重分别为11.79 g和2.76 g),在水温(28±1)℃、自然光照条件下,研究了初始体重差异对其补偿生长的影响。试验为期4周,每种规格设定3组,第1组为对照组,整个试验期间正常投喂;第2组第1周正常投喂,第2周停止投喂,后2周恢复投喂;第3组前两周2停止投喂,后2周恢复投喂。大规格鱼的3组分别以LC(对照组)、LD1和LD2表示,小鱼分别以SC(对照组)、SD1和SD2表示。结果显示:整个试验结束时,两种规格试验鱼试验组的体重均未赶上对照组,但各组的特定生长率在恢复生长过程中均显著高于对照组,表现为部分补偿生长;L规格鱼的补偿生长主要通过显著提高饲料转化效率和摄食率实现,S规格鱼通过显著提高摄食率实现;试验结束时,除LD2组的脂肪含量显著较低外,其余各组的鱼体生化成分与对照组无显著差异。     

Effects of Fumonisin B1-Containing Feed on the Muscle Proteins and Ice-Storage Life of White Shrimp (Litopenaeus vannamei)

Juvenile (5.5–5.7 g) white leg shrimp were exposed to mycotoxin in one indoor trial by feeding fumonisin B₁ (FB₁) at levels from 0.2 to 2.0 μg g^?1 FB₁ for 30 days. Shrimp growth was affected after exposure to more than 0.6 μg g^?1 FB₁. Soluble muscle protein concentration decreased, and changes in myosin thermodynamic properties were observed in shrimp after 30 days of exposure to FB₁. Marked histological changes in tissue of shrimp fed a diet containing FB₁ at 2.0 μg g^?1 were also observed. Shrimp fed diets containing more than 0.6 μg g^?1 FB₁ showed greater decrease in shear forces after 12 days of ice storage.     

Growth performance and disease resistance against Vibrio vulnificus infection of novel hybrid grouper (Epinephelus lanceolatus × Epinephelus fuscoguttatus)

Groupers are economically important for aquaculture in Thailand. A novel hybrid grouper (Epinephelus lanceolatus × Epinephelus fuscoguttatus) has been successful cross‐bred; therefore, the present work aimed to assess the hybrid's traits. The growth performance, strength and tolerance to a pathogenic bacterial infection of this hybrid were compared with its parent species, tiger grouper and giant grouper. The results of all measured growth parameters indicated that the hybrid strain grew fastest followed by giant and tiger grouper respectively. The expressions of the growth‐related genes, insulin‐like growth factor (IGF) I and II, were also analysed in fish muscle and liver which are the main target organs in fish growth regulation. Among tested species, similar expression patterns of IGF‐I and IGF‐II were detected in both organs. The levels of these genes in liver and muscle of hybrid and giant grouper were higher than those of tiger grouper comparable with the growth manner. After challenge with Vibrio vulnificus, the immunological parameters, clearance time of Vibrio in haemolymph and survival was measured to verify the fish immunity. Leucocyte number, lysozyme activity and the ability to eliminate the pathogen were very high in hybrid and giant grouper while these parameters were lower in tiger grouper. Correspondingly, the mortality rate of tiger grouper was higher than others and % survival at the end of observation time (15 days post challenge) was lowest in infected tiger grouper. Altogether, the results suggested that the hybrid grouper has desirable traits that will improve cultured grouper.     

饲料中添加蛋白酶Aquagrow对鲤生长和蛋白质消化酶活性的影响

为考察蛋白酶Aquagrow对鲤(Cyprinus carpio L)生长和蛋白质消化酶活性的影响,在鱼粉含量为6%的基础饲料中添加175 mg/kg的蛋白酶Aquagrow,饲养平均体重为48.7 g的鲤30 d.结果显示,基础饲料组(对照组)和添加蛋白酶组的鱼体增重率分别为99.4%、105.8%,饲料系数分别为1.85、1.75,添加蛋白酶提高了鲤鱼增重率6.4个百分点(P＜　0.05),降低了饲料系数5.4%(P＜　0.05),但对鱼体肌肉水分、粗脂肪、粗蛋白含量没有显著影响(P＞0.05);对消化酶活性的测定表明,添加蛋白酶可显著提高肠道组织蛋白酶活性和肠道内容物蛋白酶活性(P＜　0.05).上述研究表明,添加蛋白酶Aquagrow可提高鲤消化道蛋白酶活性,改善鲤生长性能.     

皮质醇混饲投喂对黄颡鱼幼鱼生长及外周血细胞的影响

采用皮质醇混饲投喂的试验方法,模拟自然条件下的慢性应激,观察和分析了应激激素皮质醇对黄颡鱼(Pelteobagrus fulvidraco)幼鱼生长和外周血细胞的效应。试验组饲料中皮质醇添加剂量为100 mg/kg,日投饵率为3%,试验期5周。结果表明:(1)外源皮质醇通过减少肠道黏膜皱褶和促进糖异生作用使黄颡鱼幼鱼增重受阻;(2)外源皮质醇使黄颡鱼幼鱼外周血白细胞数量显著减少、淋巴细胞百分比显著降低,但是中性粒细胞和单核细胞百分比则呈上升趋势;(3)外源皮质醇能促进红细胞增生,增加黄颡鱼幼鱼外周血红细胞数量。     

维生素B12对圆型臂尾轮虫种群增长、繁殖和个体大小的影响

为了解维生素B 12 对海水圆型臂尾轮虫( Brachionus rotundiformis )种群增长、繁殖和个体大小的影响,采用四种不同浓度的维生素B 12 (0、0.3、0.6、1.2 μg/mL)强化培养轮虫7 d。每天测定轮虫的种群密度和怀卵率,每隔一天测定轮虫背甲长和宽。试验结果显示:0.3 μg/mL维生素B 12 组轮虫的种群增长、怀卵率显著高于其他组( P<0.05);0.3 μg/mL维生素B 12 组轮虫的背甲长和宽均显著小于其他三组( P>0.05),而其他三组差异不显著。试验结论:在本试验条件下,维生素B 12 对圆型臂尾轮虫种群增长、繁殖和个体大小影响的最适浓度为0.3 μg/mL。