Establishment of a male fertility prediction model with sperm RNA markers in pigs as a translational animal modelOA

Background: Male infertility is an important issue that causes low production in the animal industry. To solve the male fertility crisis in the animal industry, the prediction of sperm quality is the most important step. Sperm RNA is the potential marker for male fertility prediction. We hypothesized that the expression of functional genes related to fertilization will be the best target for male fertility prediction markers. To investigate optimum male fertility prediction marker, we compared t...     

Supplementing the early diet of broilers with soy protein concentrate can improve intestinal development and enhance short-chain fatty acid-producing microbes and short-chain fatty acids,especially butyric acidOA

Background: Research on nutrition in early-life commonly focuses on the maturation of the intestine because the intestinal system is crucial for ensuring continued growth. To explore the importance of early nutrition regulation in animals, soy protein concentrate(SPC) was added to the early diet of broilers to investigate its effects on amino acid digestibility, intestinal development, especially intestinal microorganisms, and broiler metabolites. A total of 192 oneday-old Arbor Acres(AA) male b...     

A review of the resistome within the digestive tract of livestockOA

Antimicrobials have been widely used to prevent and treat infectious diseases and promote growth in food-production animals. However, the occurrence of antimicrobial resistance poses a huge threat to public and animal health, especial y in less developed countries where food-producing animals often intermingle with humans. To limit the spread of antimicrobial resistance from food-production animals to humans and the environment, it is essential to have a comprehensive knowledge of the role of th...     

Fecal microbiota dynamics and its relationship to diarrhea and health in dairy calvesOA

Background: Diarrhea is a major cause of morbidity and mortality in young calves, resulting in considerable economic loss for dairy farms. To determine if some gut microbes might have resistance to dysbiotic process with calf diarrhea by dictating the microbial co-occurrence patterns from birth to post-weaning, we examined the dynamic development of the gut microbiota and diarrhea status using two animal trials, with the first trial having 14 Holstein dairy calves whose fecal samples were collec...     

Oxytocin in pig seminal plasma is positively related with in vivo fertility of inseminated sowsOA

Background: Identification of relevant in vivo biomarkers for fertility remains a challenge for the livestock industry.Concentrations of the small peptide hormone oxytocin(OXT), involved in male reproductive function and present in the seminal plasma(SP) of several species could be a robust one. This study characterized concentrations of SPOXT in ejaculates from boars used in artificial insemination(AI) programs aiming to evaluate its relationship with sperm quality variables and in vivo fertili...     

Single-cell transcriptomic atlas of goat ovarian agingOA

Background The ovaries are one of the first organs that undergo degenerative changes earlier in the aging process, and ovarian aging is shown by a decrease in the number and quality of oocytes. However, little is known about the molecular mechanisms of female age-related fertility decline in different types of ovarian cells during aging, especially in goats. Therefore, the aim of this study was to reveal the mechanisms driving ovarian aging in goats at single-cell resolution.Results For the firs...     

Efficient production of chimeric mice from embryonic stem cells injected into 4- to 8-cell and blastocyst embryos

Background： Production of chimeric mice is a useful tool for the elucidation of gene function. After successful isolation of embryonic stem （ES） cell lines, there are many methods for producing chimeras, including co-culture with the embryos, microinjection of the ES ceils into pre-implantation embryos, and use of tetraploid embryos to generate the full ES-derived transgenic mice. Here, we aimed to generate the transgenic ES cell line, compare the production efficiency of chimeric mice and its proportion to yield the male chimeric mice by microinjected ES cells into 4- to 8-cell and blastocysts embryos with the application of Piezo-Micromanipulator （PMM）, and trace the fate of the injected ES cells. Results： We successfully generated a transgenic ES cell line and proved that this cell line still maintained pluripotency. Although we achieved a satisfactory chimeric mice rate, there was no significant difference in the production of chimeric mice using the two different methods, but the proportion of the male chimeric mice in the 4- to 8-cell group was higher than in the blastocyst group. We also found that there was no tendency for ES cells to aggregate into the inner cell mass using in vitro culture of the chimeric embryos, indicating that they aggregated randomly. Conclusions： These results showed that the PMM method is a convenient way to generate chimeric mice and microinjection of ES cells into 4- to 8-cell embryos can increase the chance of yielding male chimeras compared to the blastocyst injection. These results provide useful data in transgenic research mediated by ES cells.     

"乳炎必治注射液"对母畜乳房炎的疗效观察

乳炎必治注射液系纯中草药制剂。经临床大面积推广应用表明，乳炎必治试用于奶牛、奶山羊、猪、兔等家畜的乳房炎和产后疾病。共收治母猪73400头，其中治愈68300头，治愈率为93．10％；好转3300头，总有效率为97．55％。治疗母兔27600只，治愈25500只，治愈率为92．40％；好转1300只，有效率为97．10％；奶牛1200头，治愈1080头，治愈率为90．00％，好转94头，有效率为97．83％；奶山羊680只，治愈540只，治愈率为79．41％，好转11只，有效率为95．59％。同时对无名高热、产后综合症及仔畜黄、白痢亦有较好的效果。     

Analysis of High Reproductivity on Wild Yak Bull

Sperm concentration of wild yak bull is 2. 13 billion per milliliter. Survival time at 0-4 ℃ is 57hrs. After thawing survival time at 37 ℃ is 12 hrs. Resistance coefficient is 144,000. Abnormal sperm rate and acrosomal integrated rate of post-thawing is 9.17% and 87.53% respectively. Moving viscosity is 1. 169 cp. Total nitrogen is 1 437.7 mg/100 ml. Head of sperm is significantly shorter and end piece is significantly longer than that of domestic yak and yellow cattle. Activity of hyaluronidase is highly stronger than the domestic yak,and the activity of LDH is higher than the domestic yak by 48 %. Hence,the fertility of the wild yak bull is powerful and the artificial inseminated rate with domestic yak and yellow cattle is 88.9% and 71.58% respectively.     

四川野生假俭草的抗寒性比较

通过冬季对四川野生假俭草进行脯氨酸含量的测定及绿色期的观察 ,表明四川不同生态型野生假俭草的抗寒性存在差异 ,脯氨酸含量的大小与绿色期的长短呈正相关关系。在 4种生态型中 ,雅安假俭草所含脯氨酸的量最高 ,为 3 2 .84μg/g ,绿色期表现最长 ,为 3 2 3d ,而都江堰假俭草脯氨酸含量为 10 .87μg/g ,绿色期表现最短。     

高原地区矮小鸡与藏鸡屠宰试验

通过对在高原地区10只成年矮小鸡和10只成年藏鸡屠宰测定,分析结果显示:平均体重矮小鸡比藏鸡高2．2％;藏鸡公鸡全净膛率为53．9％,矮小鸡公鸡全净膛率为64．9％,差异极显著;藏鸡母鸡全净膛率为58．1％,矮小鸡母鸡全净膛率为51．5％,差异极显著;藏鸡公鸡半净膛率为74．2％,矮小鸡公鸡半净膛率为81．5％,差异显著;藏鸡母鸡半净膛率为77．1％,矮小鸡母鸡半净膛率为66．2％,差异极显著。经测定说明矮小鸡的屠宰率高于藏鸡。     

李新品种‘金山晚脆李’的选育

‘巫溪晚脆李’是从地方青脆李实生变异植株中选育出的成熟较晚、果型端庄、肉质脆嫩、离核味甜的青脆李新品种。该品种树势强健,生长快,比本地酒泉香脆李晚25d-30d,在巫溪海拔1100m区域成熟时间在八月中旬。果实较大,近圆形,平均纵径4.2cm、横径4.74cm,单果重58.7g。果皮绿色或黄绿色,皮中等厚,果点明显,果粉厚,白色。果顶略凹。果肉绿黄色,肉质硬脆,汁多味香,脆嫩,纯甜适口。可溶性固形物含量12%-15%,可滴定酸0.71％-0.84％,Ｖｃ含量1.56 mg/100g-2.85mg/100g,果核小,离核,呈扁圆形,果实可食率达97.83%-97.91％。无明显采前裂果和果内流胶,晚熟,丰产,果实硬熟时肉质松脆,但软熟后出现肉变粉质、硬度下降现象,常温下不耐贮运。耐高温高湿和寡日照气候,适应性好,适宜在重庆海拔在500m-1300m的山地地区发展。     

甘露寡糖、中药和微生态制剂对肉鸡抗氧化性能的影响

1992只1日龄AA肉鸡随机分为4个处理组,饲喂四种不同的日粮。42日龄测定各组肉鸡组织及血清抗氧化指标的水平。结果表明:(1)除心脏和血清外,各试验组MDA含量均与IV组差异显著(P<0.05)。(2)肝脏中SOD变化较明显,各试验组均高于IV组,且差异显著(P<0.05)。心脏各组之间差异不显著。(3)心脏GSH-Px含量以III、II组较高,且均与IV组差异显著。血清以II组最高,且与其他各组之间差异显著(P<0.05)。(4)心脏T-AOC含量变化较大,各试验组均高于IV组,且差异显著(P<0.05)。血清以II组最高,且与其他试验组差异显著(P<0.05)。     

高效液相色谱法测定烯丙孕素口服液中烯丙孕素的含量

建立了烯丙孕素口服液中烯丙孕素含量测定的高效液相色谱分析方法及样品前处理方法。采用正相高效液相色谱法,前处理溶剂为异丙醇-正己烷(5:20),色谱柱为ZORBAX CN C18柱(250×4.0 mm,5μm),流动相正己烷-异丙醇(98:2),流速1.0 mL/min,柱温35℃,检测波长235 nm。该方法中烯丙孕素的定量限浓度为804.9 ng/mL,线性范围为0.401~0.601 mg/mL,平均回收率为100.3%,该方法前处理简单、准确度高,可适用于该制剂中烯丙孕素的定性定量测定。     

鸡线虫对雏鸡成活率及发育的影响

通过雏鸡人工感染鸡蛔虫和异刺线虫的试验证明，鸡蛔虫、异刺线虫单独或混合感染，对雏鸡的成活率及发育均有程度不同的影响。与不感染对照组相比，感染３００个／只蛔虫卵的雏鸡成活率低１９％（Ｐ＜０．０５），死亡峰期在感染后７～１４天，增重低３０．１％（Ｐ＜０．０１），影响增重峰期为感染后第１～１０和２１～４０天；感染同量异刺线虫卵雏鸡成活率低１０．３％（Ｐ＞０．０５），死亡主因是组织滴虫病，增重低１６．６％（Ｐ＜０．０５），影响增重峰期在感染后第１１～２０天，混合感染减半量的两种虫卵，成活率低１４％（Ｐ＜０．０５），增重低３２．９％（Ｐ＜０．０１），死亡及发育受阻期与单独感染蛔虫卵组相似，但部分鸡死于组织滴虫病；单独感染减半量蛔虫卵，成活率低１７．３％（Ｐ＜０．０５），增重低１０．１％（Ｐ＞０．０５），死亡及影响增重峰期基本在感染后第１～１０天。     

闽西地区规模化猪场猪瘟抗体水平监测及免疫效果分析

应用猪瘟正向间接血凝试验 ,检测了闽西地区规模化猪场 10 93份血清的猪瘟抗体水平。结果表明 ,猪瘟血清抗体水平≤ 1∶ 8的有 2 6 7份 ,免疫合格率为 75 .5 %。哺乳仔猪检测 10 7份 ,猪瘟血清抗体水平≤ 1∶ 8的有 34份 ,免疫合格率为 6 8%。断奶仔猪检测 333份 ,猪瘟血清抗体水平≤ 1∶ 8的有 12 3份 ,免疫合格率为 6 2 %。育肥猪检测 34 0份 ,猪瘟血清抗体水平≤ 1∶ 8的有 94份 ,免疫合格率为 72 %。母猪检测 313份 ,猪瘟血清抗体水平≤ 1∶ 8的有 16份 ,免疫合格率为 95 %。所有猪群中断奶仔猪免疫合格率最低 ,与临床上断奶仔猪猪瘟发病率最高相吻合。     

RADIOGRAPHIC AND HISTOLOGIC APPEARANCE OF SYNOVIAL OSTEOCHONDROMATOSIS OF THE FEMOROTIBIAL BURSAE IN A HORSE: A CASE HISTORY REPORT

Synovial osteochondromatosis, an uncommon monarticular disease characterized by intrasynovial proliferation of cartilage cells, is described in a 16-year-old horse. Synovial osteochondromatosis is a direct metaplasia of connective tissue cells within the synovial or bursal structure. Etiology is unknown although trauma is implicated. Radiographic diagnosis is difficult and may be confused with chondrosarcoma. The presence of intra articular loose bodies and calcified granular matter is not pathognomonic for the disease. Histologic diagnosis is dependent on wide excision biopsy and serial sectioning. Radical synovectomy is the treatment of choice if extracapsular expansion is identified. Intrasynovial osteochondromatosis appears self-limiting. Cosmetic appearance and limitation of motion are the reasons treatment is sought.