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1.
Cucurbitacin-containing phytonematicides consistently reduce nematode population densities in various crops. However, there is no information on how these products affect the accumulation of essential nutrient elements in crops. The objective of this study was to determine the influence of Nemafric-BL and Nemarioc-AL phytonematicides on accumulation of essential nutrient elements in leaf tissues of green bean (Phaseolus vulgaris L.) under greenhouse conditions. Weekly application of phytonematicides each at 0%, 2%, 4%, 8%, 16% and 32%, were assessed on plant growth variables, nematode suppression and nutrient elements (Ca, K, Na, Fe, Zn) in leaf tissues of green bean at 56 days after initiating the treatments. Phytonematicide concentrations significantly (P?≤?0.05) affected nematode numbers and nutrient elements, without affecting plant growth. Calcium (R2?=?0.97), K (R2?=?0.93), Na (R2?=?0.94) and Fe (R2?=?0.91) each with increasing Nemafric-BL phytonematicide concentration exhibited positive quadratic relations. In contrast, K (R2?=?0.97) and Fe (R2?=?0.98) each with increasing Nemarioc-AL phytonematicide concentration exhibited positive quadratic relations, whereas Na (R2?=?0.92) and Zn (R2?=?0.72) each with increasing phytonematicide concentration exhibited negative quadratic relations. In conclusion, accumulation of essential nutrient elements in green bean leaf tissues and increasing phytonematicide concentrations exhibited density-dependent growth patterns prior to the eventual expression of plant growth responses.  相似文献   

2.
Experimental System: Fermented dried fruit of wild cucumber (Cucumis myriocarpus Naude.) Had been successfully used as Nemarioc-AL phytonematicide in managing plant-parasitic nematodes in various cropping systems. However, the specialised oven-drying equipment are inaccessible to most resource-poor smallholder farmers in marginalised communities. Procedures: The objective of this study was to determine the efficacy of fermented crudes extracts of C. myriocarpus fruit when used in fresh form as Nemarioc-AL phytonematicide on management of population densities of root-knot (Meloidogyne species) nematodes and growth of tomato (Solanum lycorpersicum L.) plants. Seedlings at five leaf-stage were transplanted into pots containing 2700?ml steam-pasteurised river sand and Hygromix at 3:1 (v/v) ratio. Each seedling was inoculated with approximately 3000 eggs and second-stage juveniles (J2) at transplanting. The phytonematicide was applied at 0%, 2%, 4%, 8%, 16%, 32% and 64%, arranged in a randomised complete block design, with five replications and validated in time. Results: At 56 days after treatment, nematode variables and phytonematicide concentrations exhibited negative quadratic relations, with coeffients of determination (COD) ranging from 93% to 97%. Similarly, plant variables and phytonematicide concentrations exhibited positive quadratic relations, with COD from 96% to 98%. Conclusions: In conclusion, when prepared from fresh fruit of C. myriocarpus, Nemarioc-AL phytonematicide was suitable for use in managing nematode population densities of Meloidogyne species in tomato production.  相似文献   

3.
Contribution of egg hatch inhibition in the overall reduction of Meloidogyne incognita numbers by Nemafric-BL phytonematicide was determined in vitro using a series of water-diluted phytonematicide solutions. Hatched juveniles were assessed at four different incubation periods. Thereafter, each phytonematicide solution was water-diluted 5 times and incubated for 5 days to evaluate the reversibility of egg hatch inhibition. At all exposure periods, except the shortest (24?h), concentration effects on egg hatch were highly significant with juvenile numbers versus increasing concentrations exhibiting quadratic relations. The models for the three consecutive exposure periods explain the quadratic relations by 95%, 94% and 98%, respectively. Results suggested that egg hatch inhibition was one of the mechanisms involved in reducing M. incognita population densities by Nemafric-BL phytonematicide.  相似文献   

4.
Interactive effects of alternatives to methyl bromide on root-knot (Meloidogyne species) nematodes could provide information on whether to use the products combined or singularly. The objective of this study was to investigate the interactive effects of Nemarioc-AL phytonematicide, Steinernema feltiae and Trichoderma harzianum on eggs and reproductive factors of Meloidogyne incognita race 2 under greenhouse conditions. The interactive effects of Nemarioc-AL phytonematicide (N), S. feltiae (S) and T. harzianum (T) on population densities of M. incognita were examined in a 2?×?2?×?2 factorial experiment in pots filled with steam-pasteurised soil. Each tomato seedling was inoculated with 2000 eggs and second-stage juveniles (J2), with phytonematicide and biocontrol agents applied at commercial rates. At 56 days after the treatments, the second-order (N1S1T1) interaction reduced eggs and the reproductive factor (RF) in roots by 100% and 93%, respectively. Similarly, the first-order (N1S0T1) interaction reduced the two variables by 92% and 72%, respectively, whereas N1S1T0 interaction had 63% and 55% reduction effects, respectively. Nemarioc-AL phytonematicide was compatible with S. feltiae and T. harzianum and could, therefore, be used collectively to manage population densities of M. incognita.  相似文献   

5.
接种食细菌线虫对小麦生长和N、P吸收的影响   总被引:1,自引:0,他引:1  
LI Hui-Xin  HU Feng 《土壤圈》2001,11(1):57-62
A 40-day gnotobiotic microcosm experiment was carried out to quantify the effect of bacterial-feeding nematode on plant growth and nutrient absorption. The results showed that inoculation of bacterial-feeding nematode Protorhabditis sp. stimulated the growth of wheat (Triticum aestivum) and the uptake of N. By the end of the 40-day incubation wheat biomass and N uptake in the treatment with nematode and bacteria (Pseudomonas sp.) increased by 6.5% and 5.9%, respectively, compared with bacteria alone treatment. The presence of nematode mainly accelerated the growth of aboveground of wheat, while it slightly inhibited the root development. There was little difference in plant tissue N concentration between treatments. P concentration and uptake of wheat, however, were generally reduced by nematode. It appears that the enhancement of plant growth and nitrogen uptake is attributed to the enhancement of nitrogen mineralization induced by nematode feeding on bacteria, and the reduction of phosphorous uptake is the result of weak root status and competition by bacteria immobilization.  相似文献   

6.
Deficit irrigation has been widely investigated as a valuable strategy for dry regions where water is the limiting factor in crop cultivation. Soil moisture can be one of the important factors that influence root-knot nematode (RKN) disease development. To determine how different levels of irrigation can affect disease development, irrigation regimes ranging from 20% to full field capacity (FC) were applied to tomato and eggplant plants inoculated with Meloidogyne javanica (M. javanica) under controlled conditions. In addition, in vitro bioassays were done to evaluate the effect of water potential and soil moisture content on M. javanica viability in the soil and reproduction on plant hosts. The relative egg hatching percentage decreased significantly with decreasing water potential from –0.1 to –1 MPa. The use of 80% irrigation level caused minor reductions in growth but significantly reduced nematode infection load. Nematode infection was reduced even further at lower levels of irrigation, however this also led to marked reductions in fresh and dry weights of the tomato and eggplants. Therefore, deficit irrigation could be used at a rate of 80% or 60% of FC to increase water use efficiency and reduce the level of RKN (M. javanica) infection without greatly reducing the growth performance of tomato and eggplant crops.  相似文献   

7.
A pot experiment was conducted in heat-sterilized soil to evaluate the effect of effective microorganism (EM) application on growth, yield, and nutrient uptake in Vigna radiata (L.) Wilczek var. NIAB Mung 98 in different soil amendment systems. Pot soil was amended with farmyard manure (FYM), Trifolium alexanrinum L. crop residues (TCR), and half (½NPK) and recommended dose (NPK) of chemical fertilizers. The EM application significantly enhanced shoot and root biomass in TCR-amended soil. However, grain yield was significantly enhanced in FYM, TCR, and NPK amendments by 24%, 15%, and 84%, respectively, as a result of EM application. Effect of EM application on nutrient uptake was variable with respect to soil amendment and plant growth stage. In general, EM application enhanced plant nitrogen (N), phosphorus (P), and potassium (K) nutrition in organic amendments while its effect was either negative or insignificant in chemical fertilizer amendments. Effects of EM application on plant nutrient uptake were more pronounced at maturity than at flowering stage.  相似文献   

8.
Summary The nematode community in litter and soil was examined for a year in the Chihuahuan desert, before and after supplemental rainfall application. Proportions of nematode-active or anhydrobiotic forms and population densities were determined for 3 treatments: control (natural rainfall), a single, large (25-mm) monthly irrigation pulse, and 4 smaller (6-mm) irrigations spaced at weekly intervals. In litter the greatest nematode abundance was in the 6 mm week–1 treatment (48 nematodes 20 g–1 litter). Bacteriovores and fungivores accounted for approximately 95% of the numbers and biomass in all treatments. In soil, water amendments had no significant effect (P < 0.05) on annual mean densities of total nematodes, fungivores, bacterivores, or omnivore predators. Phytophage densities were greater on both irrigation treatments, with highest densities (9268 m–2) in the 6 mm week–1 soils, which was 5.9% of the total soil nematode density. Total densities of individual trophic groups were not significantly different before or after rainfall. Soil nematode densities fluctuated independently with trophic group, month, and season. Bacterial feeders and omnivore predators were the largest contributor to total soil nematode density and biomass. Prior to irrigation, there were no differences in the percentage of anhydrobiotes on the three treatments. Anhydrobiotes decreased after irrigation in all treatments, and were significantly lower in soils of the larger, monthly irrigation. Nematodes were inactive (anhydrobiotic) and decoupled from decomposition processes when soil water matric potentials reached –0.4 MPa.Dedicated to the late Prof. Dr. M.S. Ghilarov  相似文献   

9.
Field studies were conducted to determine the influence of carbonated irrigation water on bell pepper (Capsicum annuum L.) yield, plant nutrient status, canopy carbon dioxide (CO2) concentration, and soil pH. Marketable yield, early yield, marketable fruit size distribution, and plant nutrient status were unaffected by carbonated irrigation water. Air CO2 concentration in the lowest portion of the canopy increased during irrigation events, but returned to the ambient CO2 concentration shortly after irrigation ceased. The effect of carbonated irrigation water on soil pH was marginal and unpredictable.  相似文献   

10.
ABSTRACT

A pot experiment was carried out to investigate the tolerance of cucumber plants (Cucumis sativus L.) to root-knot nematode after inoculation with Glomus intraradices. Plants were inoculated with G. intraradices for four weeks and then transplanted in soil treated with Meloidogyne incognita for a further five weeks. The low phosphorus (P) loamy soil was amended with 50 and 100 mg P kg?1 soil. Mycorrhizal colonization increased shoot dry weight, shoot length, leaf numbers, root fresh weight and shoot P concentration, whereas nematode penetration and reproduction were significantly decreased. Similarly, P fertilization usually increased shoot growth and significantly decreased the number of galls and the number of egg masses and eggs per g root. Our results indicate that inoculation with G. intraradices and P fertilizer confer tolerance of cucumber plants to M. incognita by enhancing plant growth and by suppressing reproduction and/or galling of nematodes during the early stages of plant growth.  相似文献   

11.
The field experiment studied the effect of irrigation [irrigation 15 days before sowing (DBS), irrigation 15 DBS + rice straw mulch, irrigation 7 DBS and irrigation 7 DBS + one irrigation at flower initiation] and biofertilizers [no biofertilizer (control), Rhizobium inoculation and Rhizobium inoculation + phosphate-solubilizing bacteria (PSB)] on chickpea growth. In mulch treatment, paddy straw mulch was applied at 4 t ha?1 one day after pre-sowing irrigation and was retained until sowing. Pre-sowing irrigation at 15 DBS showed 28.7 and 30.0% less plant stand than irrigation applied 15 DBS + straw mulch and irrigation applied 7 DBS, respectively. Nodulation was significantly higher with irrigation 15 DBS + mulch and irrigation 7 DBS than with irrigation applied 15 DBS. The grain yield was 16.6, 20.3 and 44.0% higher in irrigation 15 DBS + mulch, irrigation 7 DBS and irrigation 7 DBS + irrigation at flowering treatments, respectively, over irrigation at 15 DBS. Rhizobium inoculation significantly improved the number of nodules and nodule dry weight compared with no treatment. Grain yield was significantly higher with Rhizobium than in untreated plots. Water use efficiency was highest when irrigation was applied 7 DBS.  相似文献   

12.
采用沼泽红假单胞菌、保加利亚乳杆菌、酵母菌和有效微生物群组成不同组合,以pH值、浑浊度、氨氮浓度、亚硝酸盐氮含量、BOD5值和CODmn值为考察指标,研究不同微生物制剂对肉鸡屠宰废水生物降效果,结果表明:沼泽红假单胞菌、保加利亚乳杆菌和有效微生物群以25∶25∶1的比例组成的复合微生物制剂,以0.01%为添加量时效果最好。  相似文献   

13.
Field water stress is a common problem in crop production, especially in arid and semi-arid zones and it is widely hypothesized that silicon (Si) could reduce water stress in plants. We set up a greenhouse study to evaluate some silicon sources—potassium silicate (K2SiO3), calcium silicate (CaSiO3) and silica gel for growth and nutrient uptake by four grass species under adequate and deficit irrigation. The four species studied were Rhodes grass (Chloris gayana), Timothy grass (Phleum pratense), Sudan grass (Sorghum sudanense) and Tall fescue (Festuca arundinacea). For all species, the biomass yield response to applied silicon under deficit irrigation was significantly better than under adequate irrigation. The yield response of Rhodes grass across silicon sources was 205% under deficit irrigation compared with only 59% under adequate irrigation; for Sudan grass it was 49% compared with 26% and for Timothy, it was 48% compared with a mere 1%. The higher responses under deficit irrigation suggest that the plants relied more on silicon to endure drought stress. Biomass yield of individual plants also differed according to soil water levels with Timothy grass being the most sensitive to water stress as it exhibited the highest yield response (209%) to adequate irrigation. This was followed by tall fescue (122%) and Rhodes grass (97%). Sudan grass was the least affected by deficit irrigation, possibly on account of improved root mass and its natural drought tolerance. Strong associations were noted between the uptake of silicon and those of nitrogen (N) and phosphorus (P) irrespective of soil water condition, but the uptake of potassium (K) was more strongly correlated with that of Si under deficit than adequate irrigation. Improvements in plant growth following Si application could therefore be linked to enhanced uptake of major essential nutrients.  相似文献   

14.
土壤磷固定是影响石灰性磷肥肥效的主要原因。本文在田间滴灌条件下采用连续浸提的方法对液体磷肥和固体颗粒磷肥及其不同施用方法对石灰性土壤各形态无机磷含量动态变化的影响进行了研究,并比较了不同处理下加工番茄磷素营养效应。结果表明:各施肥处理0—20 cm土层Ca2-P和Ca8-P含量随施肥时间明显下降,而Ca10-P含量则显著上升,表明磷肥在石灰性土壤中不断向Ca10-P转化并被固定。液体磷肥追肥处理0—20 cm土层Ca2-P含量在各时期均显著高于其他施肥处理(P<0.05),且液体磷肥追肥可以明显保持土壤0—20 cm土层较高的Ca8-P含量。与其他施肥处理相比,液体磷肥追施可减少石灰性土壤对磷的固定,增加0—20 cm土层Ca2-P和Ca8-P含量(P<0.05),显著提高土壤磷的有效性。液体磷肥追施处理可显著提高加工番茄叶片含磷量和经济产量(91725 kg/hm2)。与传统过磷酸钙颗粒磷肥作基肥处理(CK1)相比,液体肥料全做追肥可使加工番茄经济产量提高26.7%,并明显提高了磷肥利用率。在滴灌条件下石灰性土壤上液体磷肥分次追施比传统的固体颗粒磷肥基施具有明显的优势,是一种非常具有应用前景的施肥方式。  相似文献   

15.
BackgroundSaffron cultivation is vital in the Taliouine-Taznakht regions, but the influence of agricultural practices on soil nematode communities, critical for soil health and plant productivity, is not well understood.This study characterizes nematode communities in saffron fields of the Taliouine-Taznakht regions, assessing the impact of various agricultural practices on these communities, with a focus on their diversity, functional roles, and potential as bio-indicators of soil health.A total of 163 soil samples were collected from saffron fields in Taliouine-Taznakht. Nematode communities were identified, quantified, and their functional diversity analyzed. Principal Component Analysis (PCA) was used to visualize relationships between nematode communities and sampling sites. Co-inertia analysis assessed the impact of agricultural practices on nematode diversity.The nematode communities were diverse and varied across regions. PCA identified unique nematode community compositions in different saffron fields. Omnivorous nematodes were strongly linked with Taouyalte (TA), and herbivorous ones were prevalent at Agadir Melloul (AM) and Sidi Hssaine (SH). Modern crop types, high-frequency irrigation, and alfalfa-barley rotation were positively correlated with predator nematode abundance, potentially controlling plant parasitic nematodes and encouraging nutrient cycling. Conversely, monocropping, traditional irrigation, and long plantation age correlated with reduced structure and maturity indices, suggesting a less stable ecosystem.This study unveils the intricate relationships between nematode communities in Taliouine-Taznakht saffron fields and agricultural practices. Findings indicate that specific practices, such as crop rotation and modern irrigation techniques, can foster beneficial nematode groups that improve soil health and potentially regulate harmful plant parasitic nematodes. This knowledge is crucial for crafting sustainable and effective saffron cultivation strategies.  相似文献   

16.
Using biological decomposition of plants in an agroecology system is a good method to reduce biogas slurry pollutions. The effects of biogas slurry irrigation on the growth, photosynthesis, and nutrient status of Perilla frutescens seedlings were investigated. The results indicated that biogas slurry irrigation caused a marked increase in plant height, root length, biomass of shoot and root, photosynthetic pigment content, net photosynthetic rate, transpiration rate, stomatal conductance, intercellular carbon dioxide (CO2) concentration, and water-use efficiency. Biogas slurry irrigation changed the concentration of nutrient elements of various organs.  相似文献   

17.
Abstract

Nitrogen (N) deficiency has become more common in the traditional wheat cultivation areas of southern Finland as yield potentials have increased. Based on data for the period studied (1968-88) a grain protein concentration below 11.2% in spring wheat (Triticun aestivum L.) is an indicator of N deficiency. The mean of maximum grain yield obtained was 4655 kg ha?1 when grain protein concentration exceeded 11.2%. The estimation of plant tissue N content could be an effective diagnostic tool for identifying N status in the early growth stages of spring wheat. To address the feasibility of this test, the present study was conducted in 1990-91 to determine the critical plant tissue N concentrations of three plant parts at the early double-ridge stage (Stage 2), at the stage when stigmatic branches of the carpel begin to form (Stage 7) and at pollination (Stage 10). Nitrogen was applied at rates of 0 and 110 kg N ha?1 as granular ammonium nitrate and granular slow-release-nitrogen fertilizers to establish a wide range of plant tissue N levels, grain yields and grain protein concentrations. Critical plant N levels were calculated for the different plant parts using the Cate-Nelson procedure. From this study it can be concluded that the critical N level recommended for Stage 2 is 43 g of N kg?1 dry matter of the whole plant. Critical N levels recommended for Stage 7 are 28 g of N kg?1 dry matter of the whole plant, 30 g of N kg?1 of the leaves and 13 mg total N in dry matter. Critical N levels recommended for Stage 10 are 12 g of N kg?1 of the whole plant, 23 g of N kg?1 of the leaves and 15 mg total N in dry matter.  相似文献   

18.
连作番茄根区病土对番茄生长及土壤线虫与微生物的影响   总被引:4,自引:0,他引:4  
探索连作番茄根区病土对番茄根结线虫病的诱导效果及引起连作障碍的微生态机制,可为深入了解番茄连作障碍发生机理及探究番茄连作障碍防治方法提供科学依据。本研究利用盆栽试验,测定了番茄在健康土壤及接种病土土壤中生物学特性变化及根结线虫侵染状况,并分析鉴定了土壤中微生物及线虫的种类与数量。结果表明,接种连作番茄根结线虫病株根区病土会对番茄生长及根结线虫侵染产生影响:1)番茄苗期根系根结数达9个?株~(-1),健康土壤无根结;土壤线虫数量较健康土壤增加390.4%;收获期番茄根结线虫侵染率达62.7%,病情指数为80.0%。2)番茄生长受到抑制,叶片防御酶活性降低,收获期茎叶及根系鲜质量较健康土壤分别减少50.2%及33.1%,苗期番茄叶片PPO活性较健康土壤降低15.8%,POD活性较健康土壤增加24.0%,差异均达显著水平(P0.05)。3)番茄根系更易感染有害菌,根系内病原菌甘蓝假单胞菌数量较健康土壤增加463倍,根区土壤细菌、真菌及放线菌总数分别增加46.3%、94.5%及134.0%。4)食细菌线虫、食真菌线虫及植物寄生性线虫数量分别为健康根区土壤的3.3倍、1.6倍及7.3倍,其中的植物寄生线虫95.6%为根结线虫。综上所述,接入连作番茄根结线虫病株根区病土不仅导致番茄遭受根结线虫侵染,而且会导致土壤线虫总量及植物寄生线虫所占比例大幅增加,并使番茄根系内有害细菌数量显著增加,对番茄生长造成显著抑制作用,同时影响番茄的生理生化特性,受线虫侵染番茄防御性酶活性降低,使其更易被根结线虫及病原菌侵染,番茄根区土壤线虫、微生物及根系内优势细菌的种类与数量及其之间的作用发生改变。  相似文献   

19.
Shifting sand dunes threatened the Baotou-Lanzhou railway being kept on operation smoothly seriously crossing Shapotou on the southeastern edge of the Tengger Desert (China). Artificial vegetation input was employed in Shapotou and a vegetated belt was established for stabilizing the shifting sand dunes. Nematode communities from bare soil between vegetation (ST, STI) and soil under the vegetation (ST-V, STI-V) in the vegetation belt, were investigated. All sites received natural rainfall, of which STI and STI-V received irrigation supplements. Total 43 genera were found in four types of soil samples, and Acrobeles, Acrobeloides, Chiloplacus, Panagrolaimus, Aphelenchoides and Ditylenchus were dominant genera in our study. Nematode abundance per 100 g fresh soil (47–552), the number of taxa identified (8–20), species richness (1.51–3.41), the proportion of plant feeders (0.3%–4.2%) and abundance of all cp groups responded to vegetation. The proportion of omnivores (0.5%–7%), the number of taxa identified, Shannon index (1.26–2.12), species richness, genus dominance (0.18–0.40) and abundance of cp3–5 responded to irrigation supplements. The application of PCA helped to reveal that almost all nematode taxa exhibited a positive loading on the horizontal axis, it implied that nematode abundance was generally higher on sites with planted input. Besides, it showed a vegetation gradient from bare soil between vegetation to soil under vegetation along the first axis, while an irrigation gradient along the second axis. Similarly, classification analysis based on all cased involved indicated that two-main group of nematodes was distinguished by their habitats under vegetation from bare soil. Those results suggested that vegetation input might play a more important role on nematode community composition than irrigation supplements for stabilization of shifting sand dunes. Therefore, nematode community composition could be as an indicator of stabilization of sand dune approaches of plant input with only natural rainfall versus with natural rainfall in combination with irrigation supplements in our system. However, the apparent anhydrobiotic state of the nematode fauna required special consideration in desert system, thus the utilization of the nematodes as an indicator of stabilization of sand dune regimes should be further explored on anhydrobiotic nematodes.  相似文献   

20.
With increasing demand for irrigation water, agricultural scientists and planners pay more attention to the utilization of diluted seawater as an alternative source for irrigation of crops. A greenhouse pot experiment was conducted to study how seawater stress(SS) affects growth, water content, cationic accumulation, and active ingredients in leaves of Aloe vera after 30 d of growth in nutrient media with 0%(control), 22%(22% SS), and 42%(42% SS) seawater stress. Results indicated the SS did not change dry biomass of leaves and stems, but gradually decreased biomass allocation to roots with increasing seawater stress. Na^+ and Cl^- in A. vera plant did not increase obviously with a big increase in seawater percentage due to low transpiration of Aloe vera. 42% SS decreased N concentration in most plant organs, but did not change or increased P concentration. Seawater stress tended to decrease concentrations of K^+ and Ca^2+ in A. vera. However, seawater salinity tended to increase the concentrations of aloin concentration in top(young) and middle leaves, and there was no significant effect of both stresses on aloin concentration in base(old) leaves. The 42% SS treatment decreased polysaccharide concentrations only in the base leaves, but not in top and middle leaves. In summary, supplying suitably diluted seawater for 30 d could increase the qualities and value of A. vera, without substantial effects on shoot dry biomass production.  相似文献   

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