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为了探讨采后菠萝果实黑心病发病过程中酚类物质代谢的变化规律,以感病品种‘巴厘’和抗病品种‘无刺卡因’的菠萝果实为材料,将其贮藏至25℃条件下,每隔2 d调查病情指数的变化情况,并对菠萝果肉进行取样,测定果实总酚和2,8-二羟甲基蒽醌含量的变化,同时对酚类代谢相关酶(PPO、POD、CAT、PAL)活性的变化趋势进行测定,结果表明,菠萝果实在贮藏第6 d 时,发病率达到80%,病情指数为15.24%,第8 d时,病情指数达到70%。发病部位果肉总酚和2,8-二羟甲基蒽醌含量呈先上升后下降的趋势,峰值分别出现在第6 d和第8 d,显著高于健康部位。酚类代谢相关酶中,PPO活性从第4 d开始出现了快速升高,而POD、PAL则第4 d之后呈下降趋势。伴随着菠萝黑心病的发生,果肉酚代谢发生了显著的变化,酚代谢紊乱失衡可能是病害发生的重要机理之一。 相似文献
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本研究以广西马山老黑糖及水牛乳为主要原料,乳酸菌种YO-MIX883 LYO 50DCU为发酵剂,以酸奶感官品质评分和酸奶酸度的综合评分作为考察指标,采用单因素及正交试验方法对凝固型黑糖酸奶发酵工艺进行优化,得到最佳的发酵黑糖酸奶的工艺与配方。结果表明,在黑糖添加量8%、菌种添加量3%、发酵时间8h、发酵温度43℃、水牛奶85%,发酵后在4℃条件下冷藏24h,获得的发酵酸奶的感官品质评分为92分,产品组织状态均匀,口感细腻,略带有黑糖独特风味,感官评价最佳。最佳发酵条件下制备的凝固型黑糖酸奶酸度为90.5°T、乳酸菌数为1.3×107CFU/g,蛋白质含量3.4%,脂肪含量4.6%,均符合中国乳制品工业行业规范发酵水牛乳(RHB703-2012)的要求。 相似文献
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红茶枸杞酸奶发酵工艺条件的优化及其品质测定 总被引:1,自引:0,他引:1
为了探索红茶枸杞酸奶发酵的最佳工艺条件,试验以红茶、枸杞粉、牛奶为主要原料,添加蔗糖、复合发酵剂,经发酵后制作一种新型营养酸奶,根据单因素和响应面优化试验对红茶枸杞汁、复合发酵剂、蔗糖添加量及发酵温度等进行优化,并对研制出的酸奶进行了感官、理化和微生物测定。结果表明:红茶枸杞汁添加量为8.09%,复合发酵剂添加量为0.18%,蔗糖添加量为8.03%,发酵温度为41.54℃,在此条件下制得的红茶枸杞酸奶最终感官评分为97.98分,与预测值较近,为最佳发酵工艺条件。说明用最佳发酵工艺制作的酸奶品质较佳。 相似文献
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本文概述了猪的毛色类型、猪的毛色遗传模式,着重综述了猪毛色基因分子基础的研究进展,指出存在问题并就未来发展方向做了思考。 相似文献
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Reasons for performing study: Clinical experiences indicate that centesis of the lateral compartment of the femorotibial joint is often unsuccessful. Objective: To determine the accuracy of 2 published and 2 unpublished techniques of centesis of the lateral compartment of the femorotibial joint. Hypothesis: It was hypothesised that a drug can be administered into the lateral compartment of the femorotibial joint via a diverticulum of this joint that surrounds the medial aspect of the long digital extensor tendon and that this technique is more accurate than described techniques of centesis of this compartment. Methods: Twenty‐four stifles of 12 horses were divided equally into 4 groups and a radiocontrast medium injected into the lateral compartment of the femorotibial joint of each group using a hypodermic needle inserted: 1) caudal to the lateral patellar ligament and proximal to the tibial plateau, 2) caudal to the long digital extensor tendon and proximal to the tibial plateau, 3) between the long digital extensor tendon and bone of the extensor groove of the tibia or 4) directly through the long digital extensor tendon until it contacted bone. Twelve veterinary students who had no experience using any of these techniques performed the injections. Accuracy of each technique was determined by examining radiographs obtained after injecting the contrast medium. Results: The most successful technique for arthrocentesis was by inserting a needle through the long digital extensor tendon. This approach was successful in all attempted cases, whilst other techniques had lower rates of success. Conclusions: The lateral compartment of the femorotibial joint can be accessed accurately by inserting a needle through the long digital extensor tendon as it lies within the extensor groove. Other techniques may not be as accurate for clinicians inexperienced in arthrocentesis of the lateral compartment of the femorotibial joint. 相似文献
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魏静 《四川畜牧兽医学院学报》2009,(4):28-32
在现代法律秩序中,商会自治规范是制定法的基础和必要的补充,甚至在某些方面替代了制定法;商会自治规范主要包括商会组织规范、行为规范、惩罚规范以及争端解决规范等;其效力仅及于其内部成员;商会自治规范和制定法之间存在冲突,但也存在整合的基础。 相似文献
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Marioni-Henry K Vite CH Newton AL Van Winkle TJ 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2004,18(6):851-858
A retrospective review of records of 205 cats with histologically confirmed disease of the spinal cord was performed to identify the prevalence of disease in this nonrandomly selected population of cats. Clinical records were reviewed, and age, duration of neurologic illness, and clinical and histopathologic findings in cats with spinal cord disease were abstracted. Disease processes were classified into 7 categories and 23 groups. The most common diseases affecting the spinal cord of cats were feline infectious peritonitis (FIP), lymphosarcoma (LSA), and neoplasia of the vertebral column secondarily affecting the spinal cord. Information on age, onset and duration of clinical signs, and lesion localization at the postmortem examination in cats belonging to the 7 categories of disease were analyzed to create a practical list of differential diagnoses. Cats were also subcategorized into 3 groups based on their age at death. FIP was the most common disease of cats younger than 2 years of age. LSA and vertebral column neoplasia were the most common diseases affecting cats between 2 and 8 years of age. Vertebral column neoplasia was the most common disease affecting cats older than 8 years of age. Results of this histopathologic study showed that FIP and LSA were the most common disease processes affecting the spinal cord of cats. However, at least 21 other groups of diseases and their relative prevalence were identified. 相似文献
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以国际标准强毒R株人工感染非免疫产蛋鸡,定时扑杀,分别从鼻窦、眶下孔、气管、肺、气囊、卵巢和输卵管分离MG,并收集感染鸡所产蛋分离MG。结果表明,人工感染48小时后上、下呼吸道及肺已被全面感染,96小时气囊已被感染,120小时输卵管已能分离到MG,卵巢始终分离不到MG。人工感染鸡自144小时便能在其所产蛋中分离出MG。药物治疗能在72小时内消除感染,油乳剂苗则需24天后逐渐降低蛋内MG分离率,药物卵内注射、种蛋药浴、高温处理均能杀死卵内MG,但以研制的种蛋浸泡剂药浴效果为最好。 相似文献
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根据JJF1059-1999《测量不确定度评定与表示》建立了高效液相色谱法测定鸡肉中土霉素残留测量的不确定度评定方法,评定过程包括建立测量程序、分析不确定度来源、对不确定度分量进行量化。将不确定度归类为两大类。随机效应导致的不确定度可以通过一系列具有统计意义的重复观测值进行A类评定;系统效应导致的不确定度通过各测量器具、设备的说明书、校准证书提供的参数进行B类评定。本方法鸡肉中土霉素残留量的相对合成标准不确定度约为3.9%。 相似文献
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以5个金柑属品种、枳壳、软枝酸橘及广西土柠檬实生苗为材料,进行复水成活率、旱害指数、叶片相对含水量、超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)、丙二醛(MDA)、脯氨酸等生理指标及隶属函数的综合分析,为柑橘抗性砧木品种选育提供参考。结果表明:胁迫20d复水后的成活率融安滑皮金柑和长寿金柑最高,为100%,软枝酸橘最低,为88.9%;随着干旱胁迫加剧,各品种的旱害指数呈上升趋势,长寿金柑的旱害指数最低,为0.25,软枝酸橘的旱害指数最高,为0.61;从叶片相对含水量的结果来看,长寿金柑的变化幅度最小,软枝酸橘的变化幅度最大,在第18d时分别为61.19%和37.85%;3种保护酶之间相互协同作用减轻植株受害程度,其活性变化幅度与丙二醛和脯氨酸的含量变化相似。利用隶属函数法综合分析,得出各品种的抗旱性强弱依次为长寿金柑>枳壳>融安滑皮金柑>融安金柑>山金柑>广西土柠檬>金枣>软枝酸橘。 相似文献
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分别用CIDR(孕酮阴道栓)+PMSG(处理1)和CIDR+PMSG+LH(处理2)两种处理方法对山羊进行同期发情处理。结果发现,两种处理均以撤栓后第1d发情率最高。撒栓后0-24h和24h-48h的发情率,处理1和处理2(61.4%,13.6%和48%,20%)之间差异不显著;撤栓后48h-72h的发情率,处理1(4.59,6)显著低于处理2(24%)(P〈0.05);撤栓后0~72h发情率,两种处理(79.5%,92%)无显著差异(P〉0.05)。从手术时对卵巢的观察可以看出,有大卵泡或排卵的发情羊,处理1为91.2%,处理2为100%,二者无显著差异;排卵的发情羊,处理1为(58.8%)显著低于处理2为(91.2%)(P〈0.05)。 相似文献