截获的鳞球茎茎线虫的检验鉴定

2007年、2008年和2010年,广东检验检疫技术中心分别3次从来自迪拜、也门旅客携带的大蒜和荷兰进境的郁金香种球中截获检疫性有害生物——鳞球茎茎线虫(Ditylenchus dipsaci(K(u|¨)hn,1857)Filipjev,1936)。对上述线虫进行了形态观察、测量,并对后两者的28S-D2D3的序列进行了扩增、克隆、测序,与Ditylenchus dipsaci(FJ707360-363)的同源性达99%。其中从荷兰进境的郁金香种球中截获鳞球茎茎线虫为系统内首次从贸易产品中检出,国家质检总局向荷方进行了通报,并发布《关于荷兰郁金香种球传带鳞球茎茎线虫的警示通报》。     

The spiculum: an additional useful character for the identification of Ditylenchus dipsaci and D. destructor (Nematoda: Anguinidae)

The quarantine nematodes Ditylenchus dipsaci and D. destructor are often routinely identified by morphology. This study identifies optimal conditions for specimen preparation of samples in order that the spiculum shape is clearly observable without distortion and allows the 2 species to be distinguished.     

PM 7/2 (2) Tobacco ringspot virus

Specific scope

This Standard describes a diagnostic protocol for Tobacco ringspot virus 1 This Standard should be used in conjunction with PM 7/76 Use of EPPO diagnostic protocols.

Specific approval and amendment

First approved in 2000‐09. Revision approved in 2017‐03     

PM 7/89 (2) Heterodera glycines

Specific scope

This Standard describes a diagnostic protocol for Heterodera glycines. 1 This Standard should be used in conjunction with PM 7/76 Use of EPPO diagnostic protocols. Terms used are those in the EPPO Pictorial Glossary of Morphological Terms in Nematology. 2

Specific approval and amendment

Approved in 2008–09. Revision approved in 2017–11.     

PM 7/39 (2) Aphelenchoides besseyi

Specific scope

This Standard describes a diagnostic protocol for Aphelenchoides besseyi. This Standard should be used in conjunction with PM 7/76 Use of EPPO diagnostic protocols. Terms used are those in the EPPO Pictorial Glossary of Morphological Terms in Nematology a .

Specific approval and amendment

Approved in 2003‐09. Revised in 2017‐04. This revision was prepared on the basis of the IPPC Diagnostic Protocol adopted in 2016 on Aphelenchoides besseyi, Aphelenchoides fragariae and Aphelenchoides ritzemabosi (Annex 17 to ISPM 27; IPPC, 2016 ). The EPPO Diagnostic Protocol only covers A. besseyi. It differs in terms of format but it is consistent with the content of the IPPC Standard for morphological identification for this species. With regard to molecular methods, one real‐time PCR test available in the region is included as well as DNA barcoding.     

PM 7/28 (2) Synchytrium endobioticum

Specific scope

This Standard describes a diagnostic protocol for Synchytrium endobioticum. 1 This Standard should be used in conjunction with PM 7/76 Use of EPPO diagnostic protocols.

Specific approval and amendment

Approved in 2003‐09. Revision approved in 2017‐06.     

PM 7/18(2): Monilinia fructicola

Specific scope

This standard describes a diagnostic protocol for Monilinia fructicola ¹ 1 Use of names of chemicals or equipment in these EPPO Standards implies no approval of them to the exclusion of others that may also be suitable.
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Specific approval and amendment

First approved in 2002–09. Revised in 2009–09

     

PM 7/17(2): Guignardia citricarpa

Specific scope

This standard describes a diagnostic protocol for Guignardia citricarpa. ¹ 1 Use of brand names of chemicals or equipment in these EPPO Standards implies no approval of them to the exclusion of others that may also be suitable.

Specific approval and amendment

First approved in 2002–09. Revised in 2009–09.

     

PM 7/4(2): Bursaphelenchus xylophilus

Specific scope

This standard describes a diagnostic protocol for Bursaphelenchus xylophilus ¹ 1 Use of brand names of chemicals or equipment in these EPPO Standards implies no approval of them to the exclusion of others that may also be suitable.
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Specific approval and amendment

First approved in 2000–09. Revised on 2009–09.