共查询到19条相似文献,搜索用时 62 毫秒
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2005年9~10月,辽宁省抚顺县某猪场饲养的仔猪,出现呼吸困难、精神沉郁、食欲下降、腹泻等症状,随后发生零星死亡。发病后采取一些治疗措施,但效果不十分明显。笔者通过对病猪和死亡猪病料的病原学检查并结合发病猪群的流行病学等,诊断 相似文献
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动物衣原体病的诊断方法探讨 总被引:2,自引:0,他引:2
动物衣原体病主要是由鹦鹉热衣原体(Chlamydia. psittaci)和反刍动物衣原体(C. pecorum)所引起的一种多症候传染病,而且是一种人畜共患病.衣原体所引起的病症复杂,给畜禽的生产带来了严重的损害.关于本病的诊断方法,国内外学者进行了较多研究,取得了很大进展. 相似文献
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猪衣原体病是由鹦鹉热衣原体引起的一种接触性传染病.该病的发生呈散发、地方流行性,病猪和潜伏感染的带菌猪是本病的主要传染源,另外需节肢动物如蚤、虱、螨、蜱等可为传播媒介,而定居在猪场的野鼠和野鸟可能被感染携带衣原体成为自然疫源地,构成对猪场的威胁.各种年龄的猪均能感染,但临床表现有所不同:母猪主要表现为流产、死胎、弱产、传染性不育等繁殖障碍;断奶仔猪常会出现肺炎、肠炎以及关节炎等;公猪感染后会出现睾丸炎、睾丸萎缩.本病的发生与卫生条件差、饲养密度过高、通风不良、潮湿阴冷、饲料营养不全、引水缺乏等因素有关. 相似文献
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采用间接血凝(IHA)试验对200头奶牛进行了衣原体病检测,其结果为阳性34头,阳性率为17%,表明民和县奶牛群中有衣原体病感染,且感染率较高。 相似文献
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猪衣原体病又称鹦鹉热(Psittacosis)或鸟疫(Ornithosis),是由鹦鹉热衣原体引起的一种接触性传染病,临床上表现为肠炎、肺炎、胸膜炎、心包炎、关节炎,睾丸炎、子宫感染等,母猪的子宫感染常导致流产。近年,我县不少防疫不健全的小规模猪场的生猪,在临床上出现衣原体的疑似病例,针对这种状况,2005年1月,我们在对全县20个乡镇开展疫病免疫监测的同时,进行了猪衣原体病的血清学调查,现将调查情况报告如下: 相似文献
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奶牛无浆体病PCR诊断方法的建立及应用 总被引:3,自引:0,他引:3
利用边缘无浆体(Anaplasma marginale)高度保守的msp5基因,建立了奶牛无浆体病PCR诊断方法。特异性试验表明与牛巴贝斯虫、双芽巴贝斯虫、温氏附红细胞体、大肠杆菌、金黄色葡萄球菌和牛白细胞DNA无交叉反应;敏感性试验表明可检测到约200个感染红细胞。利用该方法对黑龙江省西部多个养牛场送检的140份血样进行检测,其中曾出现过高热、气喘、流涎和贫血等临床症状的"无名高热"奶牛血样95份、无临床症状奶牛血样45份,结果有上述临床症状奶牛PCR血样阳性率为44.2%,无症状奶牛血样PCR阳性率为13.3%。从而证实无浆体是引起奶牛"无名高热"的病原之一。 相似文献
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A simplified PCR assay for fast and easy mycoplasma mastitis screening in dairy cattle 总被引:1,自引:0,他引:1
Hidetoshi Higuchi Hidetomo Iwano Kazuhiro Kawai Takehiro Ohta Tetsu Obayashi Kazuhiko Hirose Nobuhiko Ito Hiroshi Yokota Yutaka Tamura Hajime Nagahata 《Journal of veterinary science (Suw?n-si, Korea)》2011,12(2):191-193
A simplified polymerase chain reaction (PCR) assay was developed for fast and easy screening of mycoplasma mastitis in dairy cattle. Species of major mycoplasma strains [Mycoplasma (M.) bovis, M. arginini, M. bovigenitalium, M. californicum, M. bovirhinis, M. alkalescens and M. canadense] in cultured milk samples were detected by this simplified PCR-based method as well as a standard PCR technique. The minimum concentration limit for detecting mycoplasma by the simplified PCR was estimated to be about 2.5 × 103 cfu/mL and was similar to that of the standard PCR. We compared the specificity and sensitivity of the simplified PCR to those of a culture method. Out of 1,685 milk samples cultured in mycoplasma broth, the simplified PCR detected Mycoplasma DNA in 152 that were also positive according to the culture assay. The sensitivity and specificity of the simplified PCR were 98.7% and 99.7%, respectively, for detecting mycoplasma in those cultures. The results obtained by the simplified PCR were consistent with ones from standard PCR. This newly developed simplified PCR, which does not require DNA purification, can analyze about 300 cultured samples within 3 h. The results from our study suggest that the simplified PCR can be used for mycoplasma mastitis screening in large-scale dairy farms. 相似文献
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Pseudocowpox in dairy cattle 总被引:1,自引:0,他引:1
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