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绵羊人造瘤胃瘘管的手术体会 总被引:2,自引:0,他引:2
永久性瘤胃瘘是研究反刍动物消化功能试验的主要手段之一 ,广泛应用于教学和科研等实践中 ,但相关资料所介绍的手术方法不尽相同。因科研需要 ,笔者于 2 0 0 0年 5月份对12只 4~ 5月龄 ,体重为 35~ 40kg的绵羊做了永久性人造瘤胃瘘手术。术后全部绵羊的瘤胃瘘管都保持到 2个月以上。在手术过程中 ,笔者综合各种资料所介绍的方法 ,并针对实际操作进行了一定的改进 ,观察术后结果 ,对如何能使手术效果更好一些深有体会 ,现总结如下 ,供同行参考。1 手术方法所有羊只术前禁食 2 4h。麻醉采用鹿眠宝 (东北农业大学动物医学学院外科教研室… 相似文献
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反刍家畜消化道的“三位点”瘘管装置,包括瘤胃瘘管、十二指肠近端瘘管、回肠末端瘘管,是研究反刍动物营养代谢最直接有效的手段。因科研需要.笔者于2005年5月对天津市农业科学院反刍动物营养研究室5只12月龄、体重35~40 kg的绵羊,实施了消化道的“三位点”永久性人造瘘管手术。手术后瘘管伤口愈合良好,瘘管都能正常使用并保持到10个月以上。1术前准备1.1术羊准备手术羊术前禁食24 h,麻醉采用速眠新2号(解放军军事医学科学院兽医研究所研制),肌肉注射,剂量为0.01 mL/kg体重,注射后5~7 min即可进入手术3级麻醉状态。1.2药械准备1.2.1手术器… 相似文献
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血管插管手术现已被应用于许多医学教学和科研试验中.甚至是某些试验成功的先决条件。在动物营养研究方面.通过动物某内脏组织的动静脉插管.测定某种或某些营养物质的净吸收.依此观察该种营养物质在该脏器的代谢规律。在动物胎儿研究方面.动物宫内胎儿血管播管术是研究胎儿生长发育、生理生化、遗传病理等的重要手段.亦是研究人体胎儿方面以及免疫学、药理学、宫内胎儿疾病治疗学等动物模型常用手段之一。在动物疾病发病机理的血液学研究方面,通过患病动物发病器官的动静脉插管.依此研究血液中的免疫细胞在疾病的发生、 相似文献
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Six sheep were equipped with both a rumen fistula and a duodenal cannula. The duodenal cannula was made of two ordinary T shape cannulas which were vulcanized into a double T cannula. Both the rumen and the duodenal cannulas were made of soft rubber.In two experiments six different diets were fed. In the first experiment the diets consisted mainly of ammonia-treated straw (NH3-straw) plus barley or sodium hydroxide-treated straw (NaOH-straw) plus concentrate containing different N-supplements. In the second experiment, the treated straw (either NH3-straw or NaOH-straw) was fed alone. The animals in the two experiments were fed at maintenance level.Duodenal digesta were collected for periods of 12 h by inflating a balloon in the distal flange of the cannula, using pieces of foam rubber. The volume of fluid leaving the rumen was measured using the marker Gr-EDTA. The effect of digesta removal on blood constituents was studied. Three to four observations on each ration were made.Digesta flow and dry matter entering the duodenum were higher with NH3-straw than NaOH-straw fed either alone or with concentrate. However, there was a considerable variation of which a large part was of individual nature.The volume of fluid reaching the duodenum was always lower than the volume leaving the rumen, indicating a net absorption of water in the omasum and abomasum. This was estimated to be from 10.1 to 11.6 1/24 h in Experiment 1 and from 0.3 to 1.4 1/24 h in Experiment 2 (51–59 and 5–31 % of the ruminai outflow, respectively).A significant increase in plasma K and Mg and decrease in Pi and a-amino N concentration were observed due to 12 h collection of duodenal digesta. However, plasma Na, Ca, glucose and urea nitrogen concentrations remained at their pre-collection levels.Post-mortem examination of duodenum in sheep slaughtered after six and 10 months revealed a normal gut with no sign of dilatation in the immediate vicinity of the cannula. 相似文献