Increased mortality of fish due to changing Al-chemistry of mixing zones between limed streams and acidic tributaries

The present study is mainly focusing on mortality variations of fish due to changing Alchemistry of mixing zones. An artificial mixing zone was made by pumping water from a limed stream and an acidic tributary into a mixing channel. Atlantic salmon (Salmo salar L.) parr were exposed to the mixed water, limed stream water, and acidic tributary water. Mortality, blood haematocrit and plasma Cl^?-concentration were recorded. Neither mortality, nor changes in haematocrit and plasma Cl^? were observed when fish were exposed to limed water, while in both acidic and mixed water, mortalities and loss of plasma Cl^? were observed. The highest mortality rates were found within the initial part (0 to 20 s) of the mixing zone. Blood haematocrit increased only in fish exposed to acidic tributary water. Our results shows that changes in Al-chemistry and subsequent Al-polymerization occur when acidic tributary water is mixed with limed stream water. We have also demonstrated that the toxicity which can arise in mixing zones are greater than in the original acidic water before mixing. The variations in mortality observed are associated with the quality and quantity of Al-polymerization as well as ageing of the polymers.     

Aluminum toxicity to fish in acidic waters

An important consequence of acidification is the mobilization of Al from the edaphic to the aquatic environment. Elevated Al levels in acidic waters may be toxic to fish. Eggs, larvae, and postlarvae of white suckers (Catostomus commersoni) and brook trout (Salvelinus fontinalis) were exposed in laboratory bioassays to pH levels 4.2 to 5.6 and inorganic Al concentrations of 0 to 0.5 mg l^?1. Aluminum toxicity varied with both pH and life history stage. At low pH levels (4.2 to 4.8), the presence of Al (up 0.2 mg l^?1 for white suckers; 0.5 mg l^?1 for brook trout) was beneficial to egg survival through the eyed stage. In contrast, Al concentrations of 0.1 mg l^?1 (for white suckers) or 0.2 mg l^?1 (for brook trout) and greater resulted in measurable reductions in survival and growth of larvae and postlarvae at all pH levels (4.2 to 5.6). Aluminum was most toxic in over-saturated solutions at pH levels 5.2 to 5.4. The simultaneous increase in Al concentration with elevated acidity must be considered to accurately assess the potential effect of acidification of surface waters on survival of fish populations.     

Long-term changes in fish populations of acid streams and lochs in galloway South West Scotland

During 1978–79, and again in 1984, fish populations were surveyed in 22 lochs and 27 streams in Galloway, southwest Scotland. Chemical analyses of these waters and of bulk precipitation were made over the same period. The study area includes moorland catchments and catchments with young or semi-mature coniferous forest. Trout were not caught in nets set in 5 lochs which were known to contain fish in the past. Angling records also indicated a decline in catches and increased average weight of trout in two other lochs. Evidence for the decline in fish populations suggests that this process has occurred over a period of at least 50 yr. In fishless lochs and streams the levels of acidity and Al were in the range known to be toxic to fish. Stream acidity and sulphate concentrations were significantly higher in catchments with semi-mature coniferous forests. The available evidence for long-term acidification of Galloway lochs and streams is discussed and it is concluded that acid depositions are likely to be the major cause of changes in the status of fisheries in this region.     

Liming the acid lake Hovvatn,Norway: A whole-ecosystem study

Hovvatn, a 1-km² chronically-acidified lake in southernmost Norway, was treated with 200 tonne of powdered limestone in March 1981. An additional 40 tonne were added to a 0.046 km² pond (Pollen) draining into Hovvatn. The lakes were stocked with brown trout in June 1981 and in each subsequent year. At ice-out pH rose from 4.4 to 6.3 (Hovvatn) and 7.5 (Pollen), Ca and alkalinity increased, and total Al decreased by 120 μg L^?1. None of the other major ions exhibited significant changes in concentration. Total organic Cand Pincreased after liming. The phytoplankton community was dominated by chrysophytes and did not change significantly following liming. The zooplankton community was typical of acid lakes prior to liming. There was a clear succession in species dominance following treatment, although no new species immigrated to the lakes. Zoobenthos changed from a community characterized by low abundance and reduced number of species to increased abundances of oligochaetes, mayflies and chironomids. Hovvatn and Pollen were barren of fish prior to stocking. The stocked fish showed remarkably high growth rate during the first years. Liming apparently improved conditions for zoobenthos, enhancing the processing of fine detritus which in turn resulted in elevated levels of TOC and P in the lakewaters during the first year after liming. The “oligotrophication” process typical of acid lakes was temporarily reversed by liming. The interactions between groups of organisms in Hovvatn and Pollen indicates that many years are required before a new steady-state can be attained following liming.     

Aluminium chemistry in two contrasted acid forest soils and headwater streams impacted by acid deposition,Vosges mountains,N.E. France

Al chemistry was studied in two acidic watersheds, one with a podzol, the other with an acid brown soil, in the Vosges mountains (N.E. France), by analysing both leaching and centrifugation soil solutions and spring waters over 3 yr. In the podzol, Al was mobilized in the eluvial horizons under the predominant influence of organic acidity, then leached down the profile as organic and F-bound Al. Strong undersaturation with respect to proto-imogolite and imogolite showed that the proto-imogolite theory of podzolization could not apply. Al was transferred from the soil to spring water mostly as Al³⁺ and Al-F. Al³⁺, as well as additional minor species (AlOH²⁺, AlSO₄ ⁺), originated from the redissolution of the top of the spodic horizons under the influence of both soil solution acidity and the occurrence of mobile anions derived from atmospheric deposition. Conversely, in the acid brown soil, Al mobilization was regulated by nitrate and occurred mainly as Al³⁺. Most of Al was retained in the deep soil and only traces of monomeric Al reached spring water. In the podzol eluvial horizons, soil solutions were undersaturated with respect to all relevant mineral phases and their chemical composition agree with the concept of a mobilization of Al from the solid soil organic Al and a control of Al³⁺ activity by complexation reaction with the solid and soluble soil organic matter and F. In the acid brown soil, soil solutions were found to be in equilibrium with natural alunite, and the formation of this mineral, if confirmed, would account for the occurrence of 'open' vermiculites instead of the expected hydroxy-Al interlayered vermiculites. Al solubility control in surface water of both watersheds remains unclear. The Al-F species in both watersheds and the likely control of Al solubility by alunite in the acid brown soil emphasize the influence of acid deposition on Al chemistry in acid watersheds.     

Liming as an ameliorator of copper toxicity in black oat (Avena strigosa Schreb.)

This study aimed to assess the effect of liming as an ameliorator of copper (Cu) toxicity in black oats (Avena strigosa Schreb.) grown in soil with a sandy texture. The treatments were soil supplementation with increasing doses of lime (0.0, 0.5 and 1.0 g kg^?1) and two of Cu (0 and 50 mg kg^?1). Pre-germinated black oat seeds were sown for 30 days in the soil, subsequently, the plant height, root and shoot dry mass and concentration of total chlorophyll, chlorophylls a and b, carotenoids and total soluble carbohydrates and starch were analyzed. The dose of 50 mg kg^?1 soil Cu caused a toxic effect on black oat plant growth and development, affected the internal distribution of nutrients, decreased the leaf chlorophyll concentration and increased the leaf concentration of total soluble carbohydrates and starch. Increasing lime doses ameliorated the toxic effects of Cu.     

The fish fauna of mountain streams in South Korean national parks and its significance to conservation of regional freshwater fish biodiversity

The fish fauna of mountain streams in the Jiri (440 km²) and Seorak (373 km²) National Park areas of South Korea was investigated from September 1998 to May 2001. A total of 5979 fish were collected and classified into 22 families and 60 species from both national parks. At Jiri, 30 species in 12 families of fishes were collected, while at Seorak there were 42 species in 17 families. Zacco temmincki [relative abundance (RA) 47.9%] was dominant at both parks. Subdominant species were Rhynchocypris kumkangensis (RA 10.8%), Zacco platypus (RA 9.4%), and Pungtungia herzi (RA 5.3%). A total of 20 species were found to be Korean endemic species, representing seven families. The relative abundance of Korean endemic species was higher at Seorak (30.5%) than Jiri (18.5%). One exotic species (Oncorhynchus mykiss) and two species translocated outside their native catchments (Hypomesus nipponensis and Coreoperca herzi) were collected. The proportion of Korean endemic freshwater fish species in both parks (33.3%) was higher than the overall proportion on the Korean peninsula (23.6%). Within the 14 South Korean mountain-area national parks as a whole, larger parks tend to have higher fish diversity. We conclude that South Korean national parks are important for conservation of the regional fish fauna, especially for endemic and endangered species. Current threats to conservation of fishes within South Korean national parks are identified and management solutions are suggested.     

Liming and wet acid deposition effects on tree growth and nutrition: Experimental results

Results from a factorial acid treatment and liming experiment in a stand of Scots pine planted in 1968 to 1970 are presented. Field plots, 75 m² in size, were supplied with 50 mm of artificial rain 38 times from 1974 to 1981. "Rain" of varying acidities was produced using groundwater mixed with H₂SO₄. Lime was applied at four levels in 1974 in a factorial acid treatment-liming design. Tree growth and foliar nutrient concentrations were measured annually from 1974 to 1988. The soil was sampled periodically from 1975 to 1988 at mainly 3 yr intervals. Tree growth was initially stimulated by increased acid loadings. Negative effects occurred after 5 yr. Positive effects of liming developed after 9 yr. No major signs of recovery from the most acid treatments have yet been found. Treatment effects appeared to be linear, indicating no threshold values for growth reactions. Treatment effects on foliar concentrations were found for a majority of elements analyzed. Increased acid loadings decreased the Mg, Ca and Mn concentrations, while K concentrations increased — especially during later years. Liming improved the nutrient status at increased acid loadings for Mg, Ca and Mn and decreased K concentrations. Soil sampling in 1984 showed major losses of Mg, Ca and Mn by increased acid loadings, while the K content was less affected. A link seems to exist between tree growth and the Mg situation in soil and foliage.     

Liming and wet acid deposition effects on tree growth and nutrition:Experimental results

Results from a factorial acid treatment and liming experiment in a stand of Scots pine planted in 1968 to 1970 are presented. Field plots, 75 m² in size, were supplied with 50 mm of artificial rain 38 times from 1974 to 1981. “Rain” of varying acidities was produced using groundwater mixed with H₂SC₄. Lime was applied at four levels in 1974 in a factorial acid treatment-liming-design. Tree growth and foliar nutrient concentrations were measured annually from 1974 to 1988. The soil was sampled periodically from 1975 to 1988 at mainly 3 yr intervals. Tree growth was initially stimulated by increased acid loadings. Negative effects occurred after 5 yr. Positive effects of liming developed after 9 yr. No major signs of recovery from the most acid treatments have yet been found. Treatment effects appeared to be linear, indicating no threshold values for growth reactions. Treatment effects on foliar concentrations were found for a majority of elements analyzed. Increased acid loadings decreased the Mg, Ca and Mn concentrations, while K concentrations increased — especially during later years. Liming improved the nutrient status at increased acid loadings for Mg, Ca and Mn and decreased K concentrations. Soil sampling in 1984 showed major losses of Mg, Ca and Mn by increased acid loadings, while the K content was less affected. A link seems to exist between tree growth and the Mg situation in soil and foliage.     

Evaluation of three experimental low-technology approaches to acid mitigation in headwater streams

Although powered dosing devices may be effective in regulating pH and acid neutralizing capacity in acid-sensitive streams, their high costs and maintenance requirements may make them unsuitable for most sites. Three alternative approaches were tried on a mountain trout stream with pH 4.5 to 5.3 and essentially no acid neutralizing capacity: (1) A barrier of wire gabions across the stream, filled with limestone chunks, raised pH about 0.5 unit for about 2 weeks. Its effect then became undetectable. Effectiveness could have been improved by using high-Ca (>95%) stone of more uniform size (5 cm in diameter), and improving the control of leaf accumulation. No biological effects of the device were detected. (2) Metal mesh baskets fed by gravity with soda ash (sodium carbonate) briquettes worked well in raising pH by as much as 1.5 units and acid neutralizing capacity by as much as 40 ueq.L^?1 for several months. However, it proved difficult to provide adequate replenishment to keep these flow-regulated devices operating during high-flow periods. (3) To reduce consumption of soda ash, we tested devices that dissolved the briquettes in an enclosed chamber and fed the concentrated sodium carbonate solution into the stream. These provided adequate neutralization when operating properly but frequently failed due to pipe clogging and to the hardening of the briquettes into a solid mass. Studies of benthic invertebrate drift and abundance associated with the soda ash treatment showed no significant effect in a few hours or in 60 days.     

Liming and fertilization of acid forest soil: Short-term effects on runoff from small catchments

Increased atmospheric deposition of strong acids and deposition of potentially acidifying compounds (e.g. ammonium) has caused a decline in pH and exchangeable base cations in forest soils in Sweden. In recent years, attention has been paid to liming of forest soil as a method to counteract the effects of acid deposition. Experiments with liming, fertilization and woodash treatment of acid forest soils started in 1984. The aim of this study was to determine the effects of low doses of lime (500 to 1500 kg ha^–1) in combination with N fertilizers on tree growth, nutritional status of trees as well as soil, and runoff chemistry. This paper describes the short term effects of liming and fertilization on runoff from ten small catchments in two regions in south Sweden. The effects of liming were small in both areas. In the catchments fertilized with N (NH₄NO₃), a substantial leakage of various N species appeared in runoff after treatment. The increased N output was dominated by nitrate. The excess leakage of N during 2 yr after fertilization was 25 and 13% as an average of the applied N in the two study areas. The mobile nitrate increased the base cations output via runoff with 10 to 100% during 1 yr after N treatment. The runoff of Al increased with 60 to 1009, the first year in the fertilized catchments. Mobilization of cations was also influenced by ammonium, especially K that was exchanged by ammonium on the surface of the soil particles. The effects of woodash-treatment were small, however, sulfate in the ash leaked out following application and about 100% of the added sulfate was found in runoff during the first year.     

Liming and fertilization of acid forest soil: Short-term effects on runoff from small catchments

Increased atmospheric deposition of strong acids and deposition of potentially acidifying compounds (e.g. ammonium) has caused a decline in pH and exchangeable base cations in forest soils in Sweden. In recent years, attention has been paid to liming of forest soil as a method to counteract the effects of acid deposition. Experiments with liming, fertilization and woodash treatment of acid forest soils started in 1984. The aim of this study was to determine the effects of low doses of lime (500 to 1500 kg ha^?1) in combination with N fertilizers on tree growth, nutritional status of trees as well as soil, and runoff chemistry. This paper describes the short term effects of liming and fertilization on runoff from ten small catchments in two regions in south Sweden. The effects of liming were small in both areas. In the catchments fertilized with N (NH₄NO₃), a substantial leakage of various N species appeared in runoff after treatment. The increased N output was dominated by nitrate. The excess leakage of N during 2 yr after fertilization was 25 and 13% as an average of the applied N in the two study areas. The mobile nitrate increased the base cations output via runoff with 10 to 100% during 1 yr after N treatment. The runoff of Al increased with 60 to 100% the first year in the fertilized catchments. Mobilization of cations was also influenced by ammonium, especially K that was exchanged by ammonium on the surface of the soil particles. The effects of woodash-treatment were small, however, sulfate in the ash leaked out following application and about 100% of the added sulfate was found in runoff during the first year.     

Liming in relation to soil acidity and p fertilizer efficiency

Abstract

An acid brown podzolic (pH 4.0) soil of wide distribution in Ireland was limed with Ca(OH)₂ to pH values of 5.2 and 7.4. With each increment of lime significant increases in growth and F uptake by Italian ryegrass occurred, the increases being greatest with the first increment. Increasing soil pH resulted in decreased extractable aluminum and iron as well as exchangeable hydrogen. Available P as measured by various extractants increased with increasing soil pH.

The relative performance of three P fertilizers, superphosphate, phospal and basic slag was affected by liming. Basic slag was more effective than superphosphate at pH 4.0, was as effective at pH 5.2 and was less effective at pH 7.4. Phospal was poorest at each pH level. While growth and P uptake increased with increasing P levels the relative responses to the three fertilizers decreased with increasing soil pH.     

Aluminium sulphate solubility in acid forest soils in Denmark

Ion leaching in 3 sandy spruce forest soils of different origin and pH was investigated in the laboratory. Zero-tension lysimeters containing undisturbed soil columns of varying soil depth were subjected to H₂SO₄ loadings for a period of 9 weeks. The analysis of the resulting leachate supports the hypothesis that Al-sulphate minerals may form in acidic soils when exposed to acid (H₂SO₄) deposition. In the B horizon of a glaciofluvial sandy soil (pH 4.2), both H⁺ and sulphate ions were retained to maintain 2pH + PSO₄ = 11.9 in the leachate solutions. This relation between H⁺ and sulphate activity may be due to an adsorption mechanism or a precipitation mechanism. The precipitation mechanism is favored by the good fit of leachate composition to the conditions for jurbanite [AlOHSO₄] formation from gibbsite [Al(OH)₃]. In the B horizon of a sandy till at pH 3.7, the Al in soil solution (0.5 mmol L^?1) was leached with sulphate. As the sulphate load was increased, some sulphate was retained. This may also be due to the dissolution and precipitation of an Al-sulphate mineral. The ion activity products of leachate solutions from the B horizon of this soil were close to the pK_s reported for jurbanite. The conditions for the possible existence and/or formation of Al-sulphate minerals in acidic soils are discussed.     

Liming in Ontario: Short-term biological and chemical changes

The short-term results of a 5-yr experimental lake neutralization program involving whole-lake liming of Trout and Bowland Lakes are reviewed. Whole-lake liming did not prevent spring melt pulses of acid and Al in a zone situated under the ice nearshore. Liming did improve whole-lake water quality in a previously acidified lake (Bowland Lake) so that lake trout (Salvelinus namaycush) could be restocked successfully. In situ bioassays showed a statistically significant improvement in survival of overwintering lake trout eggs after liming. Lake trout fry exposed during spring melt also showed increased survival rates after liming except at one site in Trout Lake where liming did not prevent the occurrence of 100% mortality. The resident yellow perch (Perca flavescens) population in Bowland Lake exhibited short-term increases in survival and in recruitment the first year following neutralization. Growth rate responses differed among age cohorts. A preliminary shoal liming experiment in Laundrie Lake suggested that nearshore spawning shoals which are not necessarily protected by whole-lake liming may be protected by treatment with fine calcite gravel.     

Liming to mitigate acidification in freshwater ecosystems: A review of the biological consequences

The use of lime to mitigate the biological effects of surface water acidification, has recently become a widespread practice. Following a background section on liming strategies and physico-chemical effects, the observed effects of this treatment on the decomposition process and major biological components of freshwater ecosystems are reviewed. Available studies, which cover up to about 6 to 8 yr post-treatment, describe many positive biological responses to liming. These include population expansion of acid-sensitive plant and animal species, and restoration of fisheries. However, no ecosystems have so far been returned to pre-acidification status, and possible reasons for this are discussed. The frequent persistence of acid episodes in limed waters may adversely affect some species. Reductions in available P and dissolved organic C may also be limiting factors, whilst precipitated metal hydroxides are potentially toxic. When physicochemical conditions are favorable, biological recovery may be restricted by other factors, including colonization rates and the reduced genetic diversity of small residual or founding populations. If the long-term ecological consequences of liming are to be predicted, further understanding of these limitations and the stability properties of aquatic communities is necessary.     

Aluminium toxicity with sorghum genotypes in nutrient solutions and its amelioration by magnesium

Effects of Al toxicity and interaction of Al and Mg on growth of twelve sorghum (Sorghum bicolor (L.) Moench) genotypes have been studied in nutrient solutions (pH 4.2). Aluminium at 30 μM decreased biomass (dry matter yield) of the individual genotypes by factors between 1.27 and 7.36, with identical sensitivity grouping of genotypes as obtained in an earlier pot experiment with an acid soil. Resembling acid-soil stress, Al toxicity was simultaneously expressed in two independent ways, i.e. impairment of root development and induced Mg deficiency. The effect of Al on total dry matter production of the genotypes was correlated more closely with changes in specific root length (m g⁻¹ dry root) than with changes in internal Mg status. Increased Mg concentrations in the solutions (2.5 and 7.5 instead of 0.25 mM) not only decreased Al-induced Mg deficiency but also reduced the concentrations of Al in/on the roots and its damaging effect on root development. Therefore, the sorghum genotypes were less sensitive to Al at the higher Mg levels. At a high Mg concentration in the solution (7.5 mM) dry matter yield of two genotypes was even stimulated by Al.     

杀毙灵对三种鱼苗急性毒性试验的初步探讨

在室内常温、静水条件下 ,采用换水生物试验法 ,以杀毙灵对巴西鲷、建鲤、白鲢三种鱼苗进行急性毒性试验。结果表明 :杀毙灵对巴西鲷、建鲤、白鲢的安全浓度 ( m L/m3)分别为 0 .0 0 2 6、0 .0 0 36、0 .0 0 4 4。96h鱼苗对杀毙灵的耐受能力为 :白鲢 >建鲤 >巴西鲷。     

Manganese Deficiency in Canola Induced by Liming to Ameliorate Soil Acidity

Applying lime to ameliorate soil acidity has been observed to induce manganese (Mn) deficiency in canola (Brassica napus L.) crops grown on acid sandy soils near Albany and gravelly acid sands of the Great Southern Districts of southwestern Australia. These soils were often Mn-deficient in patches for wheat (Triticum aestivum L.) production when they were newly cleared for agriculture requiring application of Mn fertilizer to ensure grain yields were not reduced by the deficiency. Since then, these soils have acidified and in the 1990s, canola started to be grown on these soils in rotation with wheat and lupins (Lupinus angustifolius L.). These limed soils may now have become marginal to deficient in Mn for canola production. The effect of liming may change the effectiveness of fertilizer Mn. In addition, the effect of liming on the residual value of Mn fertilizer applied to these soils for canola production is unknown. Therefore, a glasshouse experiment was conducted using Mn deficient sand. Three levels of finely-powdered calcium carbonate were added and incubated in moist soil for 42 days at 22±2°C to produce 3 soils with different pH values [1:5 soil:0.01 M calcium chloride (CaCl₂)]: 4.9 (original soil), 6.3, and 7.5. Five Mn levels, as solutions of Mn sulfate, were then added and incubated in moist soil for 0, 50, and 100 days before sowing canola. To estimate the residual value (RV) of incubated Mn for canola production, the effectiveness of the incubated Mn was calculated relative to the effectiveness of Mn applied just before sowing canola (freshly-applied Mn). The RV of the incubated Mn was determined using yield of dried canola shoots, the Mn application level required to produce 90% of the maximum shoot yield, and Mn content in dried shoots (Mn concentration in shoots multiplied by yield of dried shoots). As measured using both yield of dried shoots and Mn content of dried shoots, the residual value of Mn decreased with increasing soil pH and with increasing period of incubation of Mn with moist soil. The critical Mn concentration, for 90% of the total yield of dried canola shoots, was (mg Mn kg^?1) ～17 in youngest mature growth (apex and youngest emerged leaf, YMG), and ～22 for the rest of dried shoots. These values were similar to current critical values for un-limed soils suggesting critical Mn concentrations remain the same for limed soils. Plant testing of canola is recommended if soils are to be limed to ameliorate soil acidity. When plant tests indicate a high likelihood of Mn deficiency, foliar Mn sprays need to be applied to that crop to ensure Mn deficiency does not reduce grain production that year, and fertilizer Mn needs to be re-applied to the soil when sowing the next crop to reduce the likelihood of Mn deficiency for subsequent crops.