猪胚胎的手术法和非手术法移植试验

试验用12头经产母猪,其中4头为供体,8头为受体。在供体母猪中,手术法和非手术法各2头。非手术法未能冲出胚胎。手术法冲出胚胎72枚,注射PMSG1200IU的1头母猪,在配种后第5天从子宫角冲出胚胎48枚,未注射PMSG的1头母猪冲出胚胎24枚。胚胎均处于囊胚阶段。移植受体母猪4头,45天均未见返情,其中1头42天流产。有3头母猪妊娠分娩,产仔最高的1窝为15头仔猪,成活12头;产仔最低的1窝为11头仔猪,成活10头。在30～32天断奶时平均成活10.67头,断奶体重和大群母猪所产仔猪无显著差异。     

一起仔猪伪狂犬病的诊治报告

1 发病情况 上杭县临城镇某养猪户,2006年1月建场,存栏母猪16头.8月15日,其中1头长大二元母猪分娩12头仔猪.据畜主诉,8月25日发现该窝仔猪突然发病,嗜睡、拉稀、体温升高.     

猪瘟并发猪肺疫的诊疗报告

宁夏中宁县宁华养殖厂共饲养．400头猪,其中母猪263头,商品猪40头,仔猪97头。2004．年7月16日该养殖场死亡母猪1头。7月20日又死亡1头。据畜主讲,20日死亡的母猪为第5胎,产后15天,产仔12头。每次仔猪出窝以8头份猪瘟疫苗、1．5头份猪肺疫疫苗防疫。     

仔猪黄痢 白痢的诊治

1 病例介绍 病例1 河北省邯郸市成安县王某饲养200头猪,其中母猪10头,均在2008年9～11月份产仔.2008年9月22日1头母猪产仔8头,产后第2天有5头仔猪开始发病并死亡2头,第3天全窝仔猪均发病,死亡3头,第4天全部死亡.     

一例仔猪黄痢的诊断与综合防治

1 发病情况 张某饲养50头猪，有10头母猪，其中5头母猪均在7、8月份产仔，第1头母猪在7月26日产下11头仔猪，第2天有2头仔猪开始发病并死亡1头，第3天全窝仔猪均发病，死亡3头，第4天死亡5头，第5天全部死亡。     

仔猪瞎眼症的调查

<正> 绍兴县龙虎山茶牧场,先后两次发生仔猪瞎眼症,现报道如下:一、发生情况1986年,该场用大约克和金华两头乌搞3元杂交,当年2月配种,6月产仔。结果有2头经产3胎的大约克纯种母猪,每窝产仔10头,其中有1半仔猪是瞎眼。有6头经产7胎的约约金母猪,共产仔50多头,全部都是瞎眼。     

双肌臀大约克猪作终端父本的洋土三元杂交育肥试验

为了研究国内近年来引进的双肌臀大约克猪的生产性能和杂交利用前景 ,笔者对其进行了杂交育肥试验和屠宰测定 ,现将试验结果报告如下。1　试验时间和分组第 1次试验是 1 998年 1 0月 1 5日～ 1 999年 1月 2 8日 ,在 3～ 7胎的长·二和大·二生产母猪中 ,选与配公猪分别为加系大约克和丹系长白的断奶仔猪各 5窝 ,每窝按体重随机选 3公 3母计 30头参加育肥试验。第 2次试验是 1 999年 9月 1 5日～1 999年 1 2月 2 9日 ,在第 2胎的双肌臀大约克和第 3～ 7胎的长·二生产母猪中 ,选纯种双肌臀大约克断奶仔猪 4窝和与配公猪分别为加系大约克和双…     

乙脑病毒引起母猪死产

我省某畜牧场于1984年由法国引进大约克种公猪两头、种母猪5头。其中有两头母猪流产期先后各产仔两窝,每窝仔猪12—13头,全部为死胎。胎儿发育正常,外表无明显变化。因怀疑为乙脑或布病,曾被淘汰处理。另外3头母猪产仔三窝,共37头,其中死胎10头,存活27头,将其培育成二代母猪14头。14头二代母猪有6头每窝均产死胎,多者11—13头仔猪全为死胎,少者每窝2—3     

母猪乳房炎致仔猪乳毒症的诊治

近年来 ,在收治的母猪乳房炎的病猪中 ,2 8头母猪就有15 6头仔猪因母猪乳房炎而致仔猪乳毒症死亡 ,其中未治死亡仔猪 5 8头 ,治疗无效死亡仔猪 2 1头。1　发病情况母猪在产仔前 3d就出现乳房炎的 7头 ,追访原因为喂霉变饲料。产后 3d出现乳房炎的 15头 ,产后 7d出现乳房炎的 6头。吮乳仔猪出现乳毒症死亡最早的为生后 7d ,最迟的为生后 30d。母猪乳房炎致仔猪乳毒症 ,最少的一窝发病 2头 ,最多的一窝发病 12头。仔猪出现病状多呈渐进性 ,未见全窝仔猪同时发病。有 5窝仔猪 39头全部陆续死亡。每窝仔猪内死亡每头间隔 1～ 3d。2　症状2 1　…     

无肛仔猪阴道瘘手术

一、病例介绍 邢台市郊区某养猪户饲养一头母猪产仔7头,5日龄时发现一头仔猪肛门处水肿,排便困难,查看后发现无肛门,且有稀便从阴道少量排出,确诊为仔猪锁肛与阴道瘘并发症,应畜主要求实行手术治疗。     

商会自治的基石——商会自治规范研究

在现代法律秩序中,商会自治规范是制定法的基础和必要的补充,甚至在某些方面替代了制定法;商会自治规范主要包括商会组织规范、行为规范、惩罚规范以及争端解决规范等;其效力仅及于其内部成员;商会自治规范和制定法之间存在冲突,但也存在整合的基础。     

癸氧喹酯干混悬剂含量测定的改进

采用高效液相色谱法测定癸氧喹酯干混悬剂的含量,在2-250μg/mL范围内,峰面积的常用对数与进样量浓度的常用对数呈良好的线性关系,R^2=1(n=5),平均回收率为99.24%~99.51%,RSD在0.05%~0.28%。此方法分析时间短,样品前处理简便、定量结果准确,重现性好,结果满意,为其质量控制提供了依据。     

猪毛色遗传的研究进展

本文概述了猪的毛色类型、猪的毛色遗传模式,着重综述了猪毛色基因分子基础的研究进展,指出存在问题并就未来发展方向做了思考。     

Comparison of 2 methods of centesis of the bursa of the biceps brachii tendon of horses

REASONS FOR PERFORMING STUDY: Centesis of the bicipital bursa using an 8.9 cm long spinal needle has been reported but the alternative of employing a 3.8 cm long hypodermic needle requires validation. OBJECTIVE: To compare the efficacy of 2 different methods of centesis of the bicipital bursa and to evaluate the usefulness of ultrasonographic imaging to determine the location of solution administered when centesis of the bursa is attempted. METHODS: For Trial 1, 6 clinicians, who had no previous experience of centesis of the bicipital bursa, attempted to inject a solution composed of an aqueous radiopaque contrast medium and physiological saline solution (PSS) into the bicipital bursae of 2/12 horses using the previously described distal approach to inject one bursa and a proximal approach to inject the contralateral bursa. The bicipital tendon and bursa were examined ultrasonographically before and after injection; and both shoulders were examined radiographically to identify the location of the medium. In Trial 2, another 6 clinicians, also with no previous experience of centesis, repeated Trial 1, using 6 horses, but the radiopaque contrast medium was mixed with air instead of PSS. RESULTS: Accuracy of centesis using the proximal approach was 39% and that of the distal approach 28%. Ultrasonographic examination of the shoulder allowed the location of solution and air to be accurately predicted in all 12 shoulders examined. CONCLUSIONS: Clinicians who have had no previous experience performing centesis of the bicipital bursa are unlikely to be successful in centesis using either approach. Radiographic examination after injecting a radiopaque contrast medium may be necessary to assess the success of centesis especially if bursal fluid is not obtained during centesis. Injecting air along with the radiopaque contrast medium provides more accurate ultrasonographic confirmation of centesis and better radiographic definition than does injection without air.     

不同样本商品蜂蜜中硒含量的测定

用硝酸和高氯酸消化蜂蜜,使硒游离出来,在微酸性环境下,硒和2,3-二氨基萘(DAN)生成有较强荧光的物质,用环己烷萃取,在激发波长378nm,荧光波长518nm处测定其荧光强度。蜂蜜中硒含量范围:0.10～0.82μg/g。表明:蜂蜜应视为天然富硒营养品。     

乳酸杆菌益生作用机制的研究进展

乳酸杆菌作为益生菌广泛用于人和动物。本文综述了乳酸杆菌改善宿主健康的机制。乳酸杆菌可通过产生抗菌物质如乳酸、过氧化氢、细菌素,或者通过竞争营养或肠道黏附位点来抑制致病菌;通过诱导黏附素的分泌或阻止细胞凋亡而增强肠道的屏障功能,从而保护肠道。文章重点讨论了乳酸杆菌表面成分（表面蛋白、脂磷壁酸和肽聚糖）与肠道受体（Ｃ型凝集素受体、Ｔｏｌｌ样受体和 Ｎｏｄ样受体）,阐述了他们结合后启动免疫调节信号,调控肠道免疫功能以发挥改善健康作用的机制。     

中华人民共和国农业部公告 第267号

为贯彻落实《兽药生产质量管理规范》(简称《兽药GMP》),进一步推动兽药GMP实施进程,我部制定了《兽药生产质量管理规范检查验收办法》,现予公告。本公告自2003年6月1日起施行。附件:兽药生产质量管理规范检查验收办法二○○三年四月十日第一章 总则 第一条 为推动《兽药生产质量管理规范》(以下简称兽药GMP)的实施,规范兽药GMP检查验收工作,制定本办法。 第二条 农业部负责全国兽药GMP管理和检查验收工作;负责制修订兽药GMP检查验收管理规定;负责兽药GMP检查员队伍建设和监督管理工作,负责国际兽药贸易中GMP互认工作。 …     

鸡败血支原体垂直传播模式及其阻断方法

以国际标准强毒Ｒ株人工感染非免疫产蛋鸡,定时扑杀,分别从鼻窦、眶下孔、气管、肺、气囊、卵巢和输卵管分离ＭＧ,并收集感染鸡所产蛋分离ＭＧ。结果表明,人工感染４８小时后上、下呼吸道及肺已被全面感染,９６小时气囊已被感染,１２０小时输卵管已能分离到ＭＧ,卵巢始终分离不到ＭＧ。人工感染鸡自１４４小时便能在其所产蛋中分离出ＭＧ。药物治疗能在７２小时内消除感染,油乳剂苗则需２４天后逐渐降低蛋内ＭＧ分离率,药物卵内注射、种蛋药浴、高温处理均能杀死卵内ＭＧ,但以研制的种蛋浸泡剂药浴效果为最好。     

Effects of size of ingestively masticated fragments of plant tissues on kinetics of digestion of NDF

Ingestively masticated fragments were collected and sized via sieving. Different sizes of esophageal masticate and ruminal digesta fragments, and ground fragments of larger masticated pieces were incubated in vitro, and undigested NDF remaining at intervals of up to 168 h of incubation was determined. The ruminal age-dependent time delay (tau) for onset of digestion of NDF was positively correlated (P < 0.004) with the mean sieve aperture estimated to retain 50% of the fragments between successive sieve apertures (MRA). Degradation rate of potentially degradable NDF (PDF) and level of indigestible NDF were not related (P > 0.10) to MRA of masticated and ground fragments. Estimates of tau were positively related to MRA, with slopes of bermudagrass < corn silage < ruminal fragments of corn silage. It was concluded that fragment size-, and consequently, ruminal age-dependent onset of PDF degradation of a mixture of different fragment sizes results in an age-dependent rate of degradation of the more rapidly degrading of two subentities of PDF. Models are proposed that assume a tau before onset of simultaneous degradation of PDF from two pools characterized as having gamma-modeled age-dependency and age-constant rates. The ruminal age-dependent pool seems to be associated with the faster-degrading pool, and its rate parameter increases with range in MRA in the population of fragments. Conceptually, the ruminal age-dependent rate parameter for PDF degradation seems to represent a composite of several effects: 1) effects of the size-dependent tau; 2) range in MRA of the population of ingestively masticated fragments; and 3) subentities of PDF that degrade via more rapid age-dependent rates compared with subentities of PDF that degrade via age-constant rates. The estimated fractional rates of ruminative comminution of ingestively masticated fragments (0.060 to 0.075/h) were of a magnitude similar to the mean fractional rates of PDF digestion (0.030 to 0.085/h), which implies that ruminative comminution may be first-limiting to fractional rate of PDF digestion. The in vivo roles of ingestive and ruminative mastication of fragments on PDF degradation must be considered in any kinetic system for estimating PDF digestion in the rumen. These results and others in the literature suggest that the rate of surface area exposure rather than intrinsic chemical attributes of PDF may be first-limiting to degradation rate of PDF in vivo.