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猪圆环病毒(PCV)是当今危害世界养猪业最严重的病毒之一,可以造成免疫抑制,引起其他相关疾病的继发和并发感染,给养猪业造成了巨大的经济损失。通过对圆环病毒的致病机理的研究进展进行总结分析,以期为猪圆环病毒病的防控提供理论依据。 相似文献
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猪圆环病毒研究进展 总被引:7,自引:0,他引:7
猪圆环病毒(Porcine Circovirus,PCV)是德国学者Tischer于1974年在多株连续传代的PK-15(ATCCCCL31)细胞中发现的一种形态类似小RNA病毒的圆形小颗粒病毒和乳多泡病毒样病毒粒子,该病毒可以持续感染PK-15细胞,不会引起细胞病变(CPE).Clark报道,1991年在加拿大的猪群中存在一种被称为猪断奶多系统消耗综合征(Postweaning Multisystemic Wasting Syndrome,PMWS)的新猪病,患病猪主要表现为进行性消瘦,呼吸困难,体表淋巴结肿大,苍白和腹泻.同年,Pejsak等首先报道在苏格兰存在一种名为猪皮炎肾病综合征(Porcine dermatitis and nephropathy syndrome,PDNS)的新猪病,其特点是全身性脉管炎和肾小球肾炎. 相似文献
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猪圆环病毒研究进展 总被引:2,自引:0,他引:2
猪圆环病毒与猪的多种疾病密切相关,可给养猪业带来巨大经济损失,因此了解其病原特征、基因组结构、流行病学、致病机理、诊断方法至关重要。本文针对这几方面对猪圆环病毒作了一个较为全面的概述,使人们更好了解这一病毒,从而加强对该病的防治,减轻其对养猪业造成的危害。1病原特征及基因组结构功能猪圆环病毒(PCV)为无囊膜单股环状负链DNA病毒,是迄今发现的最小动物病毒。PCV以滚环方式复制,呈20面体对称,无囊膜,直径17nm ̄22nm,含有1.76kb ̄2.3kb的单链环状DNA,相对分子质量58ku,沉降系数为52S。该病毒根据其抗原性及其基因组成不… 相似文献
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猪圆环病毒的致病机理及其影响因素 总被引:4,自引:0,他引:4
猪圆环病毒2型(PCV2)感染可损伤猪的免疫系统,引起感染猪的胸腺萎缩、T细胞和B细胞数量减少;细胞因子的表达量改变,如IL-10的量上升,IL-8的量明显下降,IFN-γ量减少。同时,PCV2感染的靶细胞在胎儿期是心肌细胞、肝细胞和巨噬细胞,而出生后只有巨噬细胞。因而推测猪感染PCV后,机体的免疫力下降,感染猪处于亚健康状态,其它病原因子如猪繁殖与呼吸综合征病毒、细小病毒、衣原体、猪流感、肺炎支原体和胸膜肺炎放线杆菌等,或应激因素如营养元素缺乏、免疫刺激和较差的饲养管理等多种因素都可加重PCV2感染猪的病情,进而发展成临床症状和病理变化较明显的断奶后仔猪多系统综合征、猪肾病综合征等与猪圆环病毒相关痰病。 相似文献
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Effect of porcine circovirus type 2 (PCV2) vaccination on porcine reproductive and respiratory syndrome virus (PRRSV) and PCV2 coinfection 总被引:1,自引:0,他引:1
Opriessnig T Madson DM Prickett JR Kuhar D Lunney JK Elsener J Halbur PG 《Veterinary microbiology》2008,131(1-2):103-114
The objectives were to determine if PCV2 vaccination is effective in reducing disease and lesions associated with PRRSV and PCV2 coinfection and if there is a difference between intradermal (ID) and intramuscular (IM) route of PCV2 vaccination. Seventy-four, 21-day-old pigs were randomly allocated into one of six groups. On day 0, pigs were vaccinated with 2ml Suvaxyn((R)) PCV2 One Dose (Fort Dodge Animal Health, Inc.) by intramuscular (VAC-M-COINF) or intradermal (VAC-D-COINF) routes. On day 28, pigs were either singularly (PRRSV-only, PCV2-only) or coinfected (COINF) with PRRSV and PCV2. All pigs in all groups were necropsied on day 42. All vaccinated pigs seroconverted (IgM, IgG, and neutralizing antibodies) to PCV2 between 14 and 28 days post-vaccination. After challenge, all groups inoculated with PRRSV had reduced average daily gain compared to CONTROLS and PCV2-only (P<0.001). COINF pigs had significantly (P<0.05) reduced anti-PCV2-IgG antibody levels and neutralizing antibody levels compared to both vaccinated groups. COINF pigs had more severe lung lesions compared to VAC-M-COINF (P<0.05). COINF pigs had higher amounts of PCV2 DNA in serum samples and feces (P<0.05) and increased amounts of PCV2 in lymphoid tissues (P<0.05) compared to both vaccinated groups. In summary, PCV2 vaccination was effective at inducing a neutralizing antibody response and significantly reducing PCV2-associated lesions and PCV2 viremia in pigs coinfected with PCV2 and PRRSV. Differences between intradermal and intramuscular routes of vaccine administration were not observed. 相似文献
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Patterson AR Opriessnig T 《Animal health research reviews / Conference of Research Workers in Animal Diseases》2010,11(2):217-234
Porcine circovirus type 2 (PCV2) is a small, non-enveloped, circular, single-stranded DNA virus of economic importance in the swine industry worldwide. Based on the sequence analyses of PCV2 strains, isolates can be divided into five subtypes (PCV2a-e). PCV2 is an ubiquitous virus based on serological and viremia data from countries worldwide. In addition, PCV2 DNA was discovered in archived samples prior to the first recognition of clinical disease. Recently, a worldwide shift in PCV2 subtype from PCV2a to PCV2b occurred. PCV2 DNA can be detected in fecal, nasal, oral and tonsillar swabs as well as in urine and feces from both naturally and experimentally infected pigs. PCV2 DNA can be detected early in the infectious process and persists for extended periods of time. The effectiveness of disinfectants for reducing PCV2 in vitro is variable and PCV2 is very stable in the pig environment. Limited data exist on the horizontal transmission of PCV2. Direct transmission of PCV2 between experimentally or naturally infected animals and na?ve animals has been documented and the incorporation of clinical or subclinically infected animals into a population represents a risk to the herd. Indirect transmission through the oral, aerosol or vaccine routes is likely a lesser risk for the transmission of PCV2 in most swine populations but may be worth evaluating in high heath herds. The objective of this review was to discuss data on the epidemiology and horizontal transmission of PCV2. 相似文献
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A meta-analysis was performed with the aim to identify factors with a relevant influence on the expression of clinical postweaning multisystemic wasting syndrome (PMWS) under experimental conditions. Data from 44 studies were included in the analysis. Several variables were studied: number of pigs in the experiment, intake of colostrum, serological status against porcine circovirus type 2 (PCV2), strain of PCV2 used for inoculation, the route and dose of inoculation, and use of potential triggering factors (such as co-infections, vaccinations, or immunomodulator products). Multiple correspondence analysis and log-linear regression methods were used to establish the relationships between the studied variables and the number of PCV2 infected pigs that developed PMWS. Based on the results of the meta-analysis, the most successful animal experiment aimed to develop PMWS should include: (1) colostrum-deprived pigs, (2) age of inoculation below 3 weeks, (3) high doses of PCV2 inoculum, (4) PCV2 strain from genotype 1, and (5) co-infection with another swine pathogen as a triggering factor. 相似文献
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The objective of this study was to evaluate the effect of porcine circovirus type 2 (PCV2) vaccines on PCV2-viremic and -seropositive piglets born from naturally PCV2-infected sows against postnatal PCV2 challenge. The experimental design was aimed at mimicking commercial swine rearing conditions to evaluate the response of the PCV2 vaccine on PCV2-viremic and -seropositive piglets after experimental PCV2 challenge. PCV2a (or 2b)-viremic piglets received a PCV2 vaccine at 21 days of age followed by a PCV2b (or 2a) challenge at 49 days of age (28 days post vaccination). The PCV2 vaccines elicited a high level of humoral (as measured by immunoperoxidase monolayer assay and neutralizing antibody titers) and cellular (as measured by the frequency of PCV2-specific interferon-γ-secreting cells) immune response in the PCV2-viremic piglets after vaccination even in the presence of maternally derived antibodies (MDA). The initial infection of PCV2 in the pigs was not affected by PCV2 vaccination, however the challenging PCV2 was reduced by PCV2 vaccination on PCV2-viremic pigs. The results from this study demonstrate that the PCV2 vaccine used in this study is effective at reducing PCV2 viremia and lymphoid PCV2 DNA, even for PCV2-viremic pigs with passively acquired MDA at the time of vaccination. 相似文献
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猪圆环病毒2型细胞培养适应毒株的培育和鉴定 总被引:8,自引:4,他引:8
从临床表现为仔猪断奶后多系统衰竭综合征(PMWS)淋巴组织病料,经聚合酶链式反应(PCR)证实为猪圆环病毒2型(PCV2)感染,采用无污染的猪肾细胞系(PK15)分离培养,并连续传代培育成一株细胞培养适应毒,命名为PCV2/LG株。分离毒株经细胞培养,于第25代后毒价显著升高,于第35代毒价可达10^5.6TCID 50/mL。采用免疫过氧化物酶单层细胞染色法(IPMA)、免疫电镜技术、分子克隆及核酸序列分析等鉴定表明,分离株感染细胞后病毒抗原主要分布在细胞核及细胞质中;病毒感染的阳性细胞呈散在分布,阳性细胞数可达50%以上;免疫电镜观察到与PCV2特异抗体结合形成的病毒免疫复合物呈实心小颗粒样粒子团,病毒粒子直径约为17nm;病毒抗原基因组由1768个核苷酸组成,与GenBank登录的8个PCV2基因组序列同源性达96.2%以上。用2mL的病毒细胞培养物(10^5.6TCID 50/mL)接种30日龄PCV2抗体阴性仔猪3头,可引起典型PMWS临床症状。本研究为进一步开展该病毒的致病性、疫苗免疫、诊断及分子生物学等研究奠定了基础。 相似文献
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Giuseppe Sarli Federico Morandi Serena Panarese Barbara Bacci Domenico Ferrara Carlo Bianco Laura Fusaro Maria Laura Bacci Giovanna Galeati Michele Dottori Paolo Bonilauri Davide Lelli Giorgio Leotti Thais Vila Francois Joisel Gordon Allan Cinzia Benazzi Fabio Ostanello 《Acta veterinaria Scandinavica》2012,54(1):51
Background
Since 1999, field evidence of transplacental infection by porcine circovirus type 2 (PCV2) and reproductive failure has been reported in pigs. The objective of this study was to evaluate the clinical and pathological consequences of PCV2 infection in conventional PCV2-seropositive gilts by insemination with PCV2b-spiked semen.Results
Six PCV2 seropositive gilts were inseminated with PCV2b-supplemented semen (infected) and three animals with semen and cell culture medium (controls). Only three out of the six infected animals were pregnant by ultrasonography on day 29 after insemination, while two out of the three controls were pregnant. One control gilt aborted on day 23 after insemination but not due to PVC2. Viraemia was demonstrated in four out of six infected and in one control gilt that became infected with PCV2a. Anti-PCV2 antibody titres showed dynamic variations in the infected group throughout the study. Among infected gilts, the animal with the lowest anti-PCV2 titre (1/100) at the beginning of the experiment and another that reached a similar low value during the experiment showed evident seroconversion over time and had also PCV2 positive foetuses. One placenta displayed mild focal necrosis of the chorionic epithelium positively stained by immunohistochemistry for PCV2 antigen.Conclusions
PCV2-seropositive gilts can be infected with PCV2 after intrauterine exposure and low maternal antibody titre may increase the probability of a foetal infection. 相似文献19.
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The objective of this study was to determine the amount of porcine circovirus type 2 (PCV2) shed in nasal, oral and fecal secretions over time following natural PCV2 infection. Fecal, oral and nasal swabs and blood were collected at regular intervals starting at 28 days post-farrowing (DPF) until 209 DPF from four pigs naturally infected with PCV2. PCV2 DNA was detected in all sample types. There were no differences in the amount of PCV2 DNA present in different sample types over time. PCV2 DNA was detectable in sera and secretions in pigs through 209 DPF. Natural exposure to PCV2 results in a long term infection and PCV2 is shed in similar amounts by nasal, oral and fecal routes. 相似文献