校对是期刊质量的保证--《杂交水稻》校对工作的一些体会

校对工作是编辑工作的继续和延伸 ,是确保期刊质量的重要工序。结合多年校对实践 ,总结了《杂交水稻》的校对方法和校对工作中的常见问题     

Intron removal requires proofreading of U2AF/3' splice site recognition by DEK

Discrimination between splice sites and similar, nonsplice sequences is essential for correct intron removal and messenger RNA formation in eukaryotes. The 65- and 35-kD subunits of the splicing factor U2AF, U2AF65 and U2AF35, recognize, respectively, the pyrimidine-rich tract and the conserved terminal AG present at metazoan 3' splice sites. We report that DEK, a chromatin- and RNA-associated protein mutated or overexpressed in certain cancers, enforces 3' splice site discrimination by U2AF. DEK phosphorylated at serines 19 and 32 associates with U2AF35, facilitates the U2AF35-AG interaction and prevents binding of U2AF65 to pyrimidine tracts not followed by AG. DEK and its phosphorylation are required for intron removal, but not for splicing complex assembly, which indicates that proofreading of early 3' splice site recognition influences catalytic activation of the spliceosome.     

医学期刊英文结构式摘要编校中应注意的几个问题

分析了国内医学期刊结构式英文摘要中存在的主要问题,如结构不完整,语态和时态运用不当,中英文不对应,缩略语使用不规范等,并提出了具体的解决办法。

     

Genetic dissection of transcriptional regulation in budding yeast

To begin to understand the genetic architecture of natural variation in gene expression, we carried out genetic linkage analysis of genomewide expression patterns in a cross between a laboratory strain and a wild strain of Saccharomyces cerevisiae. Over 1500 genes were differentially expressed between the parent strains. Expression levels of 570 genes were linked to one or more different loci, with most expression levels showing complex inheritance patterns. The loci detected by linkage fell largely into two categories: cis-acting modulators of single genes and trans-acting modulators of many genes. We found eight such trans-acting loci, each affecting the expression of a group of 7 to 94 genes of related function.