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鸡传染性法氏囊病实验室诊断技术研究近况(安徽农业大学)韩学毛,谢三星鸡传染性法氏囊病(IBD)是一种急性、接触性传染病。主要危害雏鸡和育成鸡。病原是传染性法氏囊病病毒(IBDV),由双股RNA组成,无囊膜。淋巴组织、法氏囊为病毒侵害的主要器官。该病发... 相似文献
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鸡传染性法氏囊病(IBD)是由鸡传染性法氏囊病病毒(IBDV)引起的一种主要危害雏鸡的免疫抑制性传染病。本病发病率高,发生本病的鸡场,常常出现新城疫、马立克氏病等疫苗接种的免疫失败,这种免疫抑制现象常使发病率和死亡率急剧上升。因此快速准确地检测鸡传染性法氏囊病病毒(IBDV),对正确诊断、预防鸡传染性法氏囊病,降低养殖户的经济损失十分重要。本文将就鸡传染性法氏囊病病毒常用的检测技术进行分类介绍。 相似文献
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鸡传染性法氏囊病(IBD)又称鸡传染性腔上囊病,是由传染性法氏囊病毒(IBDV)引起的急性、接触传染性疾病。此病主要侵害鸡的体液免疫中枢器官法氏囊等淋巴组织,因此该病的危害不仅表现在疫病本身,更重要的是引起鸡体的免疫机能障碍,影响各种疫苗的免疫应答,甚至导致免疫失败。 相似文献
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对28日龄无特定病原(SPF)鸡通过点眼、滴鼻方式人工感染了传染性法氏囊病病毒(IBDV)超强毒株GX;自感染第2天至第9天,每天用免疫胶体金试纸膜和琼脂扩散试验(AGP)两种方法检测九种组织(法氏囊、脾脏、胸腺、肾脏、肝脏、盲肠、扁桃体、肺脏、心脏、直肠内容物)的病毒含量变化,初步探索出传染性法氏囊病病毒在鸡体内各组织的侵染性变化规律。 相似文献
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用聚合酶链反应(PCR)技术直接检测鸡传染性法氏囊病病毒,可快速、特异及敏感地检出实验毒株和病鸡法氏囊内存在的微量病毒核酸。对病鸡、进口种鸡的实测结果表明,本法对诊断鸡传染性法氏囊病病毒具有重要的实用意义 相似文献
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鸡传染性法氏囊病病毒人工感染雏鸡法氏囊的病理学动态观察 总被引:3,自引:1,他引:2
鸡传染性法氏囊病病毒人工感染雏鸡法氏囊的病理学动态观察任玉红宁官宝(山西农业大学动物医学系,太谷030801)传染性法氏囊病(IBD)是鸡的一种由病毒引起的急性、接触性传染病。主要导致鸡体法氏囊淋巴细胞坏死而引起免疫抑制,使鸡对其它传染病易感性增强,... 相似文献
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魏静 《四川畜牧兽医学院学报》2009,(4):28-32
在现代法律秩序中,商会自治规范是制定法的基础和必要的补充,甚至在某些方面替代了制定法;商会自治规范主要包括商会组织规范、行为规范、惩罚规范以及争端解决规范等;其效力仅及于其内部成员;商会自治规范和制定法之间存在冲突,但也存在整合的基础。 相似文献
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本文概述了猪的毛色类型、猪的毛色遗传模式,着重综述了猪毛色基因分子基础的研究进展,指出存在问题并就未来发展方向做了思考。 相似文献
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以国际标准强毒R株人工感染非免疫产蛋鸡,定时扑杀,分别从鼻窦、眶下孔、气管、肺、气囊、卵巢和输卵管分离MG,并收集感染鸡所产蛋分离MG。结果表明,人工感染48小时后上、下呼吸道及肺已被全面感染,96小时气囊已被感染,120小时输卵管已能分离到MG,卵巢始终分离不到MG。人工感染鸡自144小时便能在其所产蛋中分离出MG。药物治疗能在72小时内消除感染,油乳剂苗则需24天后逐渐降低蛋内MG分离率,药物卵内注射、种蛋药浴、高温处理均能杀死卵内MG,但以研制的种蛋浸泡剂药浴效果为最好。 相似文献
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REASONS FOR PERFORMING STUDY: Centesis of the bicipital bursa using an 8.9 cm long spinal needle has been reported but the alternative of employing a 3.8 cm long hypodermic needle requires validation. OBJECTIVE: To compare the efficacy of 2 different methods of centesis of the bicipital bursa and to evaluate the usefulness of ultrasonographic imaging to determine the location of solution administered when centesis of the bursa is attempted. METHODS: For Trial 1, 6 clinicians, who had no previous experience of centesis of the bicipital bursa, attempted to inject a solution composed of an aqueous radiopaque contrast medium and physiological saline solution (PSS) into the bicipital bursae of 2/12 horses using the previously described distal approach to inject one bursa and a proximal approach to inject the contralateral bursa. The bicipital tendon and bursa were examined ultrasonographically before and after injection; and both shoulders were examined radiographically to identify the location of the medium. In Trial 2, another 6 clinicians, also with no previous experience of centesis, repeated Trial 1, using 6 horses, but the radiopaque contrast medium was mixed with air instead of PSS. RESULTS: Accuracy of centesis using the proximal approach was 39% and that of the distal approach 28%. Ultrasonographic examination of the shoulder allowed the location of solution and air to be accurately predicted in all 12 shoulders examined. CONCLUSIONS: Clinicians who have had no previous experience performing centesis of the bicipital bursa are unlikely to be successful in centesis using either approach. Radiographic examination after injecting a radiopaque contrast medium may be necessary to assess the success of centesis especially if bursal fluid is not obtained during centesis. Injecting air along with the radiopaque contrast medium provides more accurate ultrasonographic confirmation of centesis and better radiographic definition than does injection without air. 相似文献
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Effects of size of ingestively masticated fragments of plant tissues on kinetics of digestion of NDF
Ingestively masticated fragments were collected and sized via sieving. Different sizes of esophageal masticate and ruminal digesta fragments, and ground fragments of larger masticated pieces were incubated in vitro, and undigested NDF remaining at intervals of up to 168 h of incubation was determined. The ruminal age-dependent time delay (tau) for onset of digestion of NDF was positively correlated (P < 0.004) with the mean sieve aperture estimated to retain 50% of the fragments between successive sieve apertures (MRA). Degradation rate of potentially degradable NDF (PDF) and level of indigestible NDF were not related (P > 0.10) to MRA of masticated and ground fragments. Estimates of tau were positively related to MRA, with slopes of bermudagrass < corn silage < ruminal fragments of corn silage. It was concluded that fragment size-, and consequently, ruminal age-dependent onset of PDF degradation of a mixture of different fragment sizes results in an age-dependent rate of degradation of the more rapidly degrading of two subentities of PDF. Models are proposed that assume a tau before onset of simultaneous degradation of PDF from two pools characterized as having gamma-modeled age-dependency and age-constant rates. The ruminal age-dependent pool seems to be associated with the faster-degrading pool, and its rate parameter increases with range in MRA in the population of fragments. Conceptually, the ruminal age-dependent rate parameter for PDF degradation seems to represent a composite of several effects: 1) effects of the size-dependent tau; 2) range in MRA of the population of ingestively masticated fragments; and 3) subentities of PDF that degrade via more rapid age-dependent rates compared with subentities of PDF that degrade via age-constant rates. The estimated fractional rates of ruminative comminution of ingestively masticated fragments (0.060 to 0.075/h) were of a magnitude similar to the mean fractional rates of PDF digestion (0.030 to 0.085/h), which implies that ruminative comminution may be first-limiting to fractional rate of PDF digestion. The in vivo roles of ingestive and ruminative mastication of fragments on PDF degradation must be considered in any kinetic system for estimating PDF digestion in the rumen. These results and others in the literature suggest that the rate of surface area exposure rather than intrinsic chemical attributes of PDF may be first-limiting to degradation rate of PDF in vivo. 相似文献