硼镁营养对不同硼效率甘蓝型油菜品种苗期硼形态的影响

通过营养液培养试验,研究硼镁对不同硼效率甘蓝型油菜品种苗期硼形态的影响。结果表明,硼正常时,硼高效品种青油10号自由态和半束缚态硼含量较低效品种高,能参与转运的硼较多;硼低效品种95105束缚态(细胞壁)硼含量较高,自由态和半束缚态硼含量较低,转运、利用效率低。低硼时,硼高效品种根部束缚态硼减少,自由态和半束缚态硼增加,而叶中各形态硼分配合理,达到优化利用;反之,硼低效品种适应营养胁迫的能力较高效品种差。镁在一定程度上可以使硼向有利于其移动的形态转化,提高硼库容量,但在低硼、低镁时,却减少硼低效品种硼库容量,加剧地上部硼的缺乏。     

不同硼效率甘蓝型油菜基因型对镁的吸收,分配差异的研究

在土培条件下,研究了不同硼效率甘蓝型油菜基因型镁的吸收、分配以及硼镁关系。结果表明,镁的吸收、分配及镁硼比值等存在基因型差异,硼对镁有一定影响。镁含量高效品种〈低效品种,镁吸收量高效品种〉低效品种,提高硼水平,镁含量下降,吸收量增加。在镁的分配、运转能力上,高效品种〉低效品种,且硼对高效品种的促进作用较大。镁硼比值为高效品种〈低效品种,镁硼比值Ｂ１〉Ｂ２〉Ｂ３。     

不同硼效率甘蓝型油菜基因型对镁的吸收、分配差异的研究

在土培条件下,研究了不同硼效率甘蓝型油菜基因型镁的吸收、分配以及硼镁关系.结果表明,镁的吸收、分配及镁硼比值等存在基因型差异,硼对镁有一定影响.镁含量高效品种＜低效品种,镁吸收量高效品种＞低效品种,提高硼水平,镁含量下降,吸收量增加.在镁的分配、运转能力上,高效品种＞低效品种,且硼对高效品种的促进作用较大.镁硼比值为高效品种＜低效品种,镁硼比值B1＞B2＞B3.     

不同硼效率棉花品种木质部和韧皮部汁液中矿质养分的差异

溶液培养条件下研究硼对2个硼效率不同的棉花品种木质部、韧皮部中硼及其它矿质养分运输的影响。结果表明,缺硼使2个棉花品种木质部汁液硼含量及溢出量明显降低,低效品种降低幅度大于高效品种。供硼充足(0.5mg/L)时,2个棉花品种木质部汁液中硼浓度均小于培养液中硼浓度;缺硼(0.002mg/L)时,高效品种与低效品种木质部汁液硼浓度分别是培养液硼浓度的32.0和20.5倍。缺硼使2个棉花品种木质部汁液中钾、锰、铜、锌含量均升高,高效品种升高幅度较大;钙含量均降低,低效品种降低幅度较大;高效品种镁含量增高,低效品种降低。而2个棉花品种木质部各养分(钾、镁、钙、锰、铜、锌)溢出量均降低,低效品种降低更明显。无论在缺硼或供硼充足时,2个棉花品种韧皮部中硼浓度均极低,但韧皮部溢泌液中其它养分受缺硼影响品种间表现不同,高效品种韧皮部钾、镁、锰、铜溢出量升高,低效品种则降低;2个品种钙、锌溢出量均降低,低效品种降低幅度更大。     

不同硼效率甘蓝型油菜品种悬浮细胞的硼钙营养效应

采用细胞培养的方法 ,研究了培养基中硼钙营养水平变化对不同硼效率甘蓝型油菜品种悬浮细胞生长及硼、钙和镁含量的影响。结果表明 ,增加硼营养显著促进了悬浮细胞的生长 ,钙对悬浮细胞生长的影响因硼供应状况和品种而异。两品种间差异表现为 ,低硼的抑制效应、高硼的促进效应以及较高钙的抑制效应都是硼低效 (对缺硼敏感 )品种Bakow(原代号 9141,曾用代号 01)比硼高效 (对缺硼不敏感 )品种特早 16 (原代号 9118,曾用代号 03)明显。增硼显著降低悬浮细胞的钙含量 ;镁含量因增硼先上升而后下降。增钙对悬浮细胞中硼含量无显著影响 ,硼钙镁之间存在着复杂的关系。     

不同硼效率棉花品种果胶与不同形态硼含量的差异

溶液培养条件下研究硼(B)对不同B效率棉花品种水溶性果胶、原果胶及水溶性B、酸溶性B含量的影响.结果表明,供B充足时,B低效棉花品种各部位果胶含量均大于高效品种,不同形态B含量也有同样的规律.缺B时,高效品种各部位果胶含量一般高于低效品种.严重缺B使2个棉花品种不同形态B含量均显著降低,水溶性B变化比酸溶性B更明显,低效品种各部位酸溶性B及上部幼叶水溶性B含量降低幅度均大于高效品种,下部老叶和根中水溶性B降低幅度小于高效品种.缺B还使2个品种上部幼叶与下部老叶水溶性B含量比值增加,高效品种增加幅度大于低效品种,该比值在品种间的差异明显大于水溶性B和酸溶性B含量的差异,可以更好地反映不同品种的B效率.     

甘蓝型油菜硼营养高效在F1代的遗传研究

采用上培方法,研究了甘蓝型油菜不同硼效率品种为亲本的15个杂交组合农艺性状、棚的吸收、累积、分配和硼效率系数等性状在F1代的表现。结果表明,正反文杂交一代生育期短、抽更早,与高效亲本相近;对土壤轻度缺棚的反应也与高效亲本一致。土壤轻度缺硼时,苗期硼含量较低,累积吸收的单位棚量所形成的干物质重较高,蒙期茎中棚的相对含量高,有利于正反交杂交一代硼素向生长点的运转和再利用。硼效率系数与高效亲本达显著相关,说明甘蓝型油菜硼高效品种与低效品种间的杂交一代表现为硼高效,硼营养高效性状为显性。     

硼对不同硼效率棉花品种苗期叶片膜脂过氧化和多胺含量的影响

土培条件下研究缺硼对不同硼效率棉花品种苗期叶片膜伤害、保护酶活性及多胺化合物含量的影响。结果表明 ,遭受缺硼胁迫时 ,两棉花品种叶片膜透性和丙二醛含量增加 ,膜脂肪酸不饱和度下降 ,保护酶SOD、POD、CAT活性降低 ,但硼高效品种变化幅度比低效品种小。缺硼还导致两品种叶片中腐胺 (Put)含量显著降低 ,而低效品种降低幅度更大 ,但缺硼使高效品种叶片中精胺 (Spm)、亚精胺 (Spd)含量及多胺总量 (Spd +Spm +Put)显著升高 ,低效品种仅有升高趋势。推测硼高效品种在缺硼条件下保持较高的多胺含量是其保护酶活性较高、膜受伤害程度较轻的原因之一。     

采用嫁接试验探讨不同甘蓝型油菜品种硼效率差异机理初报

溶液培养条件下,比较研究不同B效率甘蓝型油菜品种及其相互嫁接植株苗期对B吸收分配的差异.试验表明,缺B胁迫明显降低高效、低效油菜品种及其相互嫁接植株地上部B含量和累积量,高效品种降低幅度明显小于低效品种,两者相互嫁接植株降低幅度均居于高效品种与低效品种之间.无论缺B与否,高效品种和以高效品种为砧木的植株地上部B分配率分别有高于低效品种和以低效品种做砧木植株的趋势.推断甘蓝型油菜品种B效率差异是由根系吸收能力以及向地上部运输能力共同作用的结果.     

不同硼效率甘蓝型油菜品种中硼的形态及其相互关系

采用硼高效 (9589,9590)和硼低效 (9141,95105)甘蓝型油菜品种各 2个及其 4个杂交种 (95105 9589,95105 9590 ,91419589,9141 9590)作试材 ,研究了不同硼效率甘蓝型油菜品种及其F1代花期各部分叶片中硼的形态。结果表明 ,在B2 (0 .35mg/kg)处理下 ,硼高效品种上叶 ,中叶 ,下叶和花中水溶态硼含量和束缚态硼含量低 ,半束缚态硼含量高 ,而硼低效品种 3种硼形态的含量则与此相反 ;F1代基本居于二者之间 ,且趋向于高效品种 ;在B1(0 .2 0mg/kg)处理下 ,各叶片和花中各硼形态含量也呈现类似的特征 ,但存在例外 ,尤其在花中。B1和B2处理下 ,除B1处理上叶外 ,硼高效品种和F1代的花和叶片中水溶态硼和束缚态硼相对含量较低 ,半束缚态硼相对含量较高 ,硼低效品种与此相反。建立了硼形态之间的相互平衡关系 ,并把这种平衡关系同硼效率联系起来 ,表明硼形态和硼效率存在密切关系 ,可望由此揭示不同甘蓝型油菜品种硼效率差异的生理机制。     

不同硼效率棉花品种根系参数和伤流液组分的差异

溶液培养条件下研究硼对不同硼效率棉花品种根系参数、伤流量及伤流液组分的影响。结果表明,缺硼抑制棉花根系生长,根重、根体积、根长、活跃吸收面积、总吸收面积、活跃吸收面积占总吸收面积的比例以及伤流量均显著降低,高效品种降低幅度比低效品种小;缺硼还使高效品种根系比表面积升高,而低效品种无显著变化。缺硼显著影响不同棉花品种伤流液各组分含量,与低效品种相比,高效品种伤流液中有较多的NO3-、K+、无机磷、可溶性糖和游离氨基酸。说明缺硼条件下高效品种根系比低效品种具有更强的吸收、代谢活力,可能是其硼效率较高的主要原因之一。     

油菜硼高效的遗传

Field experiments were conducted to study the inheritance of boron efficiency in oilseed rape (Brassica napus L.) by evaluating the boron (B) efficiency coefficient (BEC, the ratio of the seed yield at below the critical boron level to that at the boron-sufficient level) with 657 F_2:3 lines of a population derived from a cross between a B-efficient cultivar, Qingyou 10, and a B-inefficient cultivar, Bakow. Qingyou 10 had high BEC as well as high seed yield at low available soil B. On the contrary, Bakow produced low seed yield at low B status. Boron deficiency decreased the seed yield of the F_2:3 lines to different extents and the distribution of BEC of the population showed a bimodal pattern. When the 657 F_2:3 lines were grouped into B-efficient lines and B-inefficient lines according to their BEC, the ratio of B-efficient lines to B-inefficient lines fitted the expected ratio (3:1), indicating that one major gene controlled the B-efficiency trait. 127 F_2:3 lines selected from the population at random, with distribution of BEC similar to that of the overall population, were used to identify the target region for fine mapping of the boron efficiency gene.     

Calcium and L-histidine interaction on nutrients accumulation in three tomato cultivars under nickel stress

This study was carried out to evaluation of some nutrients accumulation including Ni (nickel), Fe (iron), Mg (magnesium), Cu (copper), Ca (calcium) as well as Ni translocation factor (TF) in three tomato cultivars grown hydroponically under treatments including L-histidine (His) and calcium chloride (CaCl₂) (300 µM) exposed to nickel (II) sulfate (NiSO₄) (150 and 300 µM) by ICP-OES (inductively coupled plasma atomic emission spectroscopy) analysis. Tomato cultivars included Cal-J N3, Early Urbana Y and. Results indicated that shoot Ni accumulation increased under Ni stress. Ca and His reduced root, shoot Ni content and Ni TF (translocation factor) under Ni stress in Petoearly CH cultivar. Ni stress also decreased root and shoot Ca, Fe, Mg and Cu while Ca and His improved the nutrients uptake, and inhibited Ni accumulation. Therefore, Ca and His alleviated adverse effects of Ni stress on nutrients in Petoearly CH cultivar. Beside, Ca and His interaction alleviate Ni stress by inhibition of Ni uptake and translocation as well as plant nutrition improvement in the tomato plants.     

Physiological and genetic responses to boron deficiency in Brassica napus: A review

Abstract

Boron (B) is an essential microelement for the growth and development of plants, and B deficiency affects many biochemical and physiological processes. Brassica napus L. has a high demand for B and is extremely sensitive to B deficiency. Seed yields and oil quality of B. napus are often limited by the low availability of B in soils. Developing new cultivars of B. napus with high B efficiency is therefore required, which requires a greater understanding of responses to B deficiency. Significant genotypic differences in response to low soil B have been observed among varieties of B. napus. B-efficient genotypes can grow and yield normally and usually have a larger root system than B-inefficient genotypes at low B conditions. The mechanisms for B efficiency in B. napus are attributed to B absorption, transportation and utilization. In addition, the cell wall component plays an important role in the tolerance of B. napus to B deficiency, and the B-efficient line presents fewer B-binding sites in the cell walls compared with the B-inefficient line. Genetic and proteomic analyses in B. napus revealed the modulation of a complex network in response to B deficiency. This review gives a comparative overview of the physiological and genetic responses to B deficiency in B. napus and discusses the possible underlying mechanisms of B efficiency.     

土壤-烤烟矿质营养元素相互关系的主组分分析

对云南五种植烟土壤上烤烟三个品种、六个生育期烟叶中钾、磷、硫、钙、镁、铁、锰、锌、铜、硼浓度和烟株根区土壤中ＡＢ／Ｄ联合提取的养分含量及土壤ｐＨ、有机质和碱解氮的测试数据，用主组分分析（ＰＣＡ）方法，讨论土壤─烤烟系统矿质营养元素的相互关系及交互作用。结果显示：①烟叶矿质营养元素浓度随烤烟生育期有规律的变化，受根区土壤化学性质影响的变异大于品种间的差异；②烟叶钾、磷、铜、硼表现明显的稀释效应，钙、镁表现积累效应；③烤烟钾、磷、铜、硼营养存在相互协同作用，钙对钾、磷、铜、硼营养有明显的拮抗作用。④增加土壤有机质，利用钾、磷、铜、硼的协同作用，减轻钙对钾、磷、硼的拮抗是改善烤烟营养，提高烤烟产量品质的关键之一。     

Influence of Cadmium Toxicity on Growth and Antioxidant Enzyme Activity in Rice Cultivars with Different Grain Cadmium Accumulation

Abstract

The effect of cadmium (Cd) toxicity on growth, lipid peroxidation, and antioxidant enzymes was studied using two rice cultivars, Bing 97252 with low and Xiushui 63 with high grain Cd accumulation. Plants were exposed to 0–5 μ M Cd in hydroponic culture. Cadmium stress inhibited plant height and chlorophyll content and altered melondialdehyde (MDA) content and the activities of superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD). Roots and shoots responded differently to Cd in terms of antioxidant enzyme activity. Generally, the activities of SOD, POD, and CAT decreased with increase in Cd level, while the activity of MDA increased with increase in Cd level. With the increase in Cd concentration in nutrient solution, MDA content in shoots and roots of Xiushui 63 increased at a much higher rate than did that of Bing 97252 at both growth stages. At booting stage, a decrease of 46%–52% in SOD activity was noted in plant roots grown under 5 μ M Cd, while at tillering stage the decrease was 13%–19% compared with the control. A significant decrease in chlorophyll content and plant height was noted under higher Cd treatment (1.0 and 5.0 μ mol) at two stages. The higher MDA and lower chlorophyll content in the cultivar Xiushui 63 showed that it is more sensitive to Cd than the cultivar Bing 97252.     

高铝低磷胁迫对胡枝子生长及矿质元素吸收的影响

限制酸性土壤作物生长的最重要、最普遍的因子是Al3+ 的毒害和 P 的缺乏.本文用溶液培养试验研究两种不同生态型的二色胡枝子在高Al低P胁迫下的矿质营养元素积累情况.试验表明,江西胡枝子比河北胡枝子更耐低 P 低 pH 的生长环境,但两者间耐Al性无显著差异;100 μm/L Al 处理显著地抑制了两种胡枝子对 Ca 的吸收,降低了根系 Mg 的积累量,对植株的 K、P、Fe、Zn、Cu 含量没有显著影响;低 P 处理没有显著降低两种胡枝子对 Ca、K、Fe、Zn、Cu 和江西胡枝子对 Mg 的吸收,但是低 P 处理显著降低了河北胡枝子对 Mg 的吸收和转运.二色胡枝子植株吸收的 Al 主要积累在根部,地上部分Al含量仅是根系的1% 左右.     

Nickel toxicity in two durum wheat cultivars differing in drought sensitivity

The effects of nickel (Ni) on growth, leaf water status, and mineral nutrient concentration were studied in two wheat (Triticum durum Desf.) cultivars with different sensitivity to water stress: ‘Adamello’ [drought sensitive (DS)] and ‘Ofanto’ [drought tolerant, (DT)]. The DT cultivar showed a higher Ni absorption capacity: ‘Ofanto’ seedlings grown in the presence of 35 μM Ni had a 3.5 times greater concentration of Ni in roots than did ‘Adamello’. Despite the greater Ni tissue content, the DT cultivar exhibited better growth and nutritional status when compared to the DS cultivar. In the DS cultivar the concentration of chlorophyll a and b was reduced by Ni treatment. Chlorophyll a concentration decreased in the DT cultivar, but to a lesser extent than in the DS cultivar; chlorophyll b was not altered by Ni level in the DT cultivar. Nickel caused a decrease in the water potential (ψ_w) and relative water content (RWC) in both cultivars, but these decreases were greater in the DS cultivar. The antioxidative defense enzymes, guaiacol peroxidase, ascorbate peroxidase, and glutathione reductase, showed increased activity in Ni‐treated DS seedlings; this increase in activity was not observed in the DT seedlings. These data suggest that different wheat genotypes may markedly differ in Ni uptake and sensitivity and that a enhanced capacity to counteract Ni stress may be associated with drought resistance.