在施钾条件下灌溉水稻的养分吸收和利用效率研究

Potassium is one of the most important nutrients for rice production in many areas of Asia, especially in southeast China where potassium deficiency in soil is a widespread problem. Field experiments were conducted for four consecutive years in Jinhua City, Zhejiang Province, to determine utilization of nutrients (N, P and K) by inbred and hybrid rice and rice grain yields as affected by application of potassium fertilizer under irrigated conditions. Grain yield and nutrient harvest index showed a significant response to the NPK treatment as compared to the NP treatment. This suggested that potassium improved transfer of nitrogen and phosphorus from stems and leaves to panicles in rice plants. N and P use efficiencies of rice were not strongly responsive to potassium, but K use efficiency decreased significantly despite the fact that the amount of total K uptake increased. A significant difference between varieties was also observed with respect to nutrient uptake and use efficiency. Hybrid rice exhibited physiological advantage in N and P uptake and use efficiency over inbred rice. Analysis of annual dynamic change of exchangeable K and non-exchangeable K in the test soil indicated that non-exchangeable K was an important K source for rice. Potassium application caused an annual decrease in the concentration of available K in the soil tested, whereas an increase was observed in non-exchangeable K. It could be concluded that K fertilizer application at the rate of 100 kg ha^-1 per season was not high enough to match K output, and efficient K management for rice must be based on the K input/output balance.     

Incorporation of rice straw mitigates CH4 and N2O emissions in water saving paddy fields of Central Vietnam

This study evaluated the effects of rice straw and water regimes on CH₄ and N₂O emissions from paddy fields for two rice growing seasons (summer 2014 and spring 2015). Water regimes included alternating wet–dry irrigation (AWD) maintained at three levels (–5 cm, – 10 cm and –15 cm) in comparison to continuous flooding irrigation (CF). Rice straw (5 t ha^–1) was incorporated into the top soil (0 – 15 cm), distributed and burned in situ. Results showed that using burned in situ rice straw was found to reduce seasonal cumulative CH₄ emission (24–34% in summer; 18–28% in spring), N₂O emission (21–32% in summer; 22–29% in spring) and lower rice yield (8–9%) than rice straw incorporation into top soil. AWD methods reduced the amount of CH₄ production (22.6–41.5%) and increased N₂O emission (25–26%) without any decrease in rice yield. Rice straw incorporation into the top soil with AWD had higher water productivity (23–37%) than rice straw when burned in situ with CF. The results conclude that AWD and rice straw management can be employed as mitigation strategy for CH₄ and N₂O emissions from paddy fields in Central Vietnam.     

控制灌溉水稻叶片水平的水分利用效率试验研究

根据现场试验资料,分析了晚稻叶片水平的水分利用效率的日变化与全生育期变化,叶片的水分利用效率与气孔导度及外界影响因子包括光合有效辐射、土壤水分、叶气温差等的相互关系。结果表明：控制灌溉水稻叶片的水分利用效率在较高土壤水分时与对照处理差别不明显,适度土壤水分调控可以获得较高的水分利用效率;全生育期水稻叶片的水分利用效率随土壤水分变化而波动,过高过低的土壤水分均不利于水分利用效率的提高;叶片的光合速率、蒸腾速率与水分利用效率均随气孔导度的增加表现出先增后减的变化规律。水稻叶片的水分利用效率影响因素分析显示：水分利用效率随叶气温差、二氧化碳浓度和空气湿度的增加而增加;有利于获得较高水分利用效率的气孔导度、光合有效辐射、空气温度和土壤水分范围分别是200～350 μmol·m^-2·s^-1、400～900 μmol·m^-2·s^-1、28～34℃和85%～90%的土壤饱和含水率。     

Effect of biochar amendment on soil‐silicon availability and rice uptake

Rice growth and its resistance to pests had been often constrained by soil‐silicon (Si) availability. The purpose of this study was to assess the potential of biochar soil amendment (BSA) to improve Si availability in paddy soils. A cross‐site field trail with BSA was conducted in six locations with different climatic and crop‐production conditions across S China. Plant‐available Si content before field‐trials establishment and after rice harvest, as well as Si content in rice shoot were determined. Varying with site conditions, plant‐available Si content of soil was observed to increase significantly with BSA in most sites. Significant increase in rice shoot Si was detected in four out of the six sites, which was well correlated to the concurrent increase in soil pH under BSA treatment. This study demonstrates an important role of BSA to improve Si availability and uptake by rice mainly through increasing soil pH of the acid and slightly acid rice soils.     

Excessive application of farmyard manure reduces rice yield and enhances environmental pollution risk in paddy fields

The present study examined the effect of excessive application of farmyard manure (FM) on rice production and environmental pollution in paddy fields of Japan. A long-term field experiment was conducted during the period 1976–2006 to examine the trends of rice yield and yield components as affected by the excessive FM application (20 Mg ha^?1 year^?1 containing 110 kg N, 180 kg P₂O₅, and 320 kg K₂O). Rice growth, soil fertility, and surface water quality were also assessed in the final year (2006). The results obtained were compared with those of a conventional practice with recommended doses of inorganic fertilizer (IF), i.e. 85 kg N, 68 kg P₂O₅, and 53 kg K₂O ha^?1 year^?1, and an unfertilized control (CR). The excessive FM application resulted in a gradual decrease in grain yield, which was mostly explained by the reduction of grain fertility under the luxuriant rice growth. This reduction may have been due to the higher accumulation of soil nutrients such as N, P, and K. Moreover, the excessive FM application increased chemical oxygen demand, total P, and soluble K concentrations in the paddy surface water and their effluent loads compared to the conventional practice with the recommended IF application.     

有机肥对磷的吸附-解吸的影响及在红壤发育成的水稻土上的有效性

A field test with the traditional rotation of paddy rice/upland crop (wheat) was carried out on a paddy soil derived from red earth to elucidate the effect of organic manure on the phosphorus adsorption-desorption by soil and its P availability. Soil samples were taken from different treatments at rice harvesting stage and analysed. The isothermal adsorption of P by the samples fitted very well with Langmuir equation, and hence, the parameters in the equation, i.e., maximum adsorption (qm), constant related to bonding energy (k) and their product (k × qm) could be used as a comprehensive index to characterize the potential P adsorptivity of the soil. Organo-inorganic fertilization and organic manuring could decrease qm and k, while mineral P application had little effect on them. The isothermal desorption of P was significantly correlated with initially added and isothermally adsorbed P. Part of P added was fixed, which represented the P fixation capacity of soil, and organic manuring could obviously lower the P fixation. The content of soil available P had a significant negative correlation with qm, k and fixed P. It is concluded that organic manure could increase the P availability of paddy soil derived from red earth by decreasing qm, k, maximum buffering capacity (MBC=k × qm) and fixation capacity.     

水稻间歇灌溉研究: Ⅰ. 水分利用率

The effects of individual and combined additions of urea(100μg N g^-1soil) and insecticide (triazophos at field rate,FR) under different moisture levles of air-dried soil(AD),50% of water-holding capacity(WHC),100%,WHC and flooded soil(FS) on some selected soil properties in a paddy field soil were examined in a laboratory incubation study.The results indicated that after 21-day incubation at 25℃ ，the different moisture levels led to significant changes in the parameters studied,Flooding of soil with distilled waer significantly increased the electron transport system(ETS)/dehydrogenase activity and phenol content of the soil compared to the other moisture levels,while protein and phospholipis behaved differently at varied moisture levels with or without the addition of urea and /or triazophos.Increased ETS activity was observed with N addition at higher moisture levels thile insecticide incorporation decreased it at all moisture levels as compared to the control(moisture only).The phenol contents slightly decreasd and increased with N and insecticide applications ,respectively,The soil protein contents were found to be unaffected among all the soil treatents at all moisture levels.The soil protein contents were found to be unaffected among all the soil treatments at all moisture levels.However,among different moisture levels,reduced quantities of proteins were estimated at 50% WHC ,suggesting more N-mineralization.Lower quantities of soil biomass phospholipids,among all treatments,were recored at higher moisture levels(100% WHC and FS) than at the loer levels,An overall slight enhancement in phospholipid contents with N and small reduction with insecticide addition,respectively,was noticed against the untreated soil.The toxictiy of fertilizer and insecticide decreased as the soil moisture contents increased,suggesting rapid degradation of agrochemicals.     

煤基复混肥对复垦土壤养分、玉米产量及水肥利用的影响

以工矿区固体废弃物为基本原料,与化肥复混研制而成煤基复混肥,通过单施煤基复混肥及其与菌肥配施在复垦区进行了大田试验,旨在研究不同施肥处理对复垦土壤养分、玉米产量、水肥利用效率的影响。结果表明:不同施肥处理均可有效促进土壤养分含量;菌肥+煤基复混肥处理使土壤有效磷含量显著高于单施复混肥或基质+煤基复混肥处理。玉米产量随施肥量的增加而增大,且在N300施肥水平达到最高,表现为菌肥+煤基复混肥(N300,5 957.52kg/hm~2)基质+煤基复混肥(N300,5 695.73kg/hm~2)单施煤基复混肥(N300,5 391.15kg/hm~2)。过量施肥(N390)导致玉米产量不同程度降低。菌肥+煤基复混肥不同处理在N210、N300和N390施肥水平玉米产量显著高于单施复混肥(P0.05)。煤基复混肥及其与菌肥配施在N300施肥水平水分利用效率最高,表现为菌肥+煤基复混肥[N300,18.32kg/(mm·hm~2)]基质+煤基复混肥[N300,17.57kg/(mm·hm~2)]单施煤基复混肥[N300,16.73kg/(mm·hm~2)]。随施肥量的增加,肥料利用率呈明显下降趋势,菌肥+煤基复混肥处理在N120、N210和N300各施肥水平的氮、磷、钾肥利用率均显著高于单施复混肥。综上所述,本研究对促进工矿区固体废弃物农业资源化利用有一定意义。     

南方低产黄泥田与高产灰泥田基础地力的差异

【目的】黄泥田为南方红黄壤区广泛分布的低产田。定量评价黄泥田基础地力,明确与高产灰泥田水稻氮磷钾养分吸收利用的差异,可为黄泥田改良及水稻施肥提供依据。【方法】采集福建省20个县的典型黄泥田0—20cm土壤,同时采集与其邻近且由同一微地貌单元发育的高产灰泥田土壤,进行了水稻盆栽试验。试验设施肥(每盆N0.60g、P2O50.24g、K2O0.42g)和不施肥两个处理。氮肥采用15N丰度10%的尿素,磷肥用磷酸二氢钙,钾肥用氯化钾。水稻品种为‘中浙优1号’,采用移栽种植,每盆种植两穴。收获后,调查籽粒产量,采集土壤和植株样品,植株分地上部和根部,分析了生物量、氮磷钾含量,土壤分析了全氮含量。植株与土壤同位素氮用ZHT-03质谱仪测定15N%丰度。计算了土壤基础地力与水稻养分吸收、累积及肥料利用率。【结果】黄泥田的基础地力经济产量、基础地力地上部生物产量较灰泥田分别低26.9%与23.5%,相应的基础地力贡献率分别低14.1与9.7个百分点。基础地力贡献率(经济产量)与土壤有机质含量呈极显著正相关,与土壤容重呈极显著负相关。不论施肥与否,黄泥田水稻有效穗数均显著低于灰泥田,且不施肥水稻有效穗数与土壤有机质含量呈极显著正相关,而与土壤容重呈极显著负相关。施肥条件下,黄泥田水稻成熟期籽粒、茎叶和根系氮、磷、钾素含量均低于灰泥田,其中3个部位磷素含量较灰泥田分别低9.6%、38.4%和46.3%,差异均显著,黄泥田水稻籽粒和茎叶的钾素含量较灰泥田分别低10.8%和18.5%,差异均显著。施肥下黄泥田水稻成熟期籽粒、茎叶的氮素吸收量较灰泥田分别低10.8%和17.3%,磷素吸收量分别低12.5%和46.2%,钾素吸收量分别低16.6%和28.5%,差异均显著。等量施肥条件下,黄泥田的水稻氮肥利用率较灰泥田低4.6个百分点,但土壤氮肥残留率增加3.0个百分点。【结论】以高产灰泥田为标准,黄泥田基础地力具有20%以上的产量提升潜力。土壤有机质与容重是影响基础地力贡献率与有效穗的重要肥力因子。黄泥田水稻氮肥利用率显著低于灰泥田,但土壤氮素残留率较高。提高有机质、降低土壤容重是提升基础地力的主攻方向。     

长期施肥稻田土壤基础地力和养分利用效率变化特征

【目的】 研究长期不同施肥措施对土壤基础地力和氮磷钾养分吸收利用效率的影响,探明土壤基础地力和氮、磷、钾养分吸收利用效率的相互关系。 【方法】 采集双季水稻种植制度下 33 年长期定位施肥试验的不施肥 (CK)、施氮磷钾肥 (NPK) 和氮磷钾肥配施稻草 (NPKS) 3 个处理的土壤,设置施肥与不施肥盆栽试验,监测水稻产量、土壤基础地力产量和基础地力贡献率、水稻氮磷钾养分吸收量、氮磷钾养分利用效率,分析氮、磷、钾利用效率对土壤基础地力贡献率的响应。 【结果】 早晚稻土壤基础地力产量和基础地力贡献率三个处理土壤大小顺序均为 NPKS > NPK > CK,NPKS 处理土壤早晚稻两季平均基础地力产量和基础地力贡献率较 CK 处理土壤分别增加 113.8% 和 93.7%,NPK 处理分别增加 100.7% 和 81.9%。在同一施肥水平条件下,早、晚稻均以土壤基础地力较高的 NPKS 处理氮、磷、钾肥偏生产力,土壤养分依存率,氮、磷、钾素收获指数较高,氮、磷、钾肥回收利用率,肥料农学效率,肥料对产量的贡献率则较低。回归分析表明,氮、磷肥回收利用率,氮、磷、钾肥农学效率,氮、钾素生理利用率均随土壤基础地力贡献率的提高呈显著或极显著降低;氮、磷、钾肥偏生产力,氮、磷、钾素土壤依存率随土壤基础地力贡献率的提高呈显著或极显著提高。 【结论】 长期施氮磷钾肥或长期氮磷钾肥配施稻草均能提高土壤基础地力,以长期氮磷钾肥配施稻草的效果更显著。在较高基础地力土壤上生产,可以在保证作物高产稳产的情况下实现减量化施肥,实现农业生产的可持续性。      

等养分条件下稻草还田替代双季早稻氮钾肥比例的研究

【目的】研究等量氮磷钾养分投入条件下,长期稻草原位全量还田配施化肥对双季早稻氮 (N)、磷 (P)、钾 (K) 养分吸收累积、转运及养分利用的影响,为南方稻区稻草资源有效利用,维持并提高土壤肥力及水稻合理施肥提供理论依据。【方法】以江西温圳国家级耕地质量监测点长期定位试验为研究对象,陆两优 996 为供试材料,在不施化肥和施用化肥基础上设稻草还田、稻草烧灰还田和稻草不还田共 6 个处理,除空白对照外,所有处理养分投入量相等。于 2015 年在早稻移栽期、分蘖期、幼穗分化期、抽穗期和成熟期取地上部植株样,分析水稻植株不同部位的 N、P、K 含量和累积量以及转运比例,并计算 N、P、K 养分利用效率。【结果】稻草还田提高了水稻产量,施肥条件下稻草还田处理比稻草烧灰还田和稻草不还田处理平均增产 2.9%～ 6.4%,比不施肥区产量增幅高达 23.8%～ 26.0%,且差异达显著水平。无论是施肥区处理,还是无肥区处理,与稻草烧灰还田和稻草不还田处理相比,稻草还田植株中 K 含量及 N、P、K 积累量在整个生育期均较高,而 N、P 含量在生育后期较高,N、P、K 积累量以施肥区处理大于相应的无肥区处理;稻草还田提高水稻 N、P、K 养分农学效率、回收率和养分偏生产力,且 N、K 差异达显著水平,同时显著增加 K 的收获指数;稻草还田还提高了抽穗至成熟期茎鞘中 N、P、K 的转运量、转运率及转移养分对籽粒的贡献率,而叶片各处理间差异不显著。【结论】稻草还田配施化肥能提高水稻产量,同时还可以调节 N、P、K 养分的积累和转运,提高养分的吸收利用效率。本试验条件下,稻草还田可替代化肥氮肥 (N) 29.5%、磷肥 (P₂O₅) 4.0% 和钾肥 (K₂O) 50.0%。综合考虑,稻草还田相比稻草烧灰还田而言是南方稻区土壤养分管理实现高效利用的有效途径之一。     

秸秆还田下氮肥运筹对白土田水稻产量和氮吸收利用的影响

【目的】研究小麦秸秆直接还田条件下不同氮肥基追比例运筹方式对白土稻田水稻产量和氮素吸收利用的影响, 为华中低产白土稻田水稻合理施肥提供科学依据。【方法】设置2种小麦秸秆还田量(0和3000 kg/hm2)及3种氮肥基肥-分蘖肥-穗肥施用比例(80-0-20、 60-20-20 和40-30-30)和不施氮的对照, 共7个处理, 分别为N80-0-20、 N60-20-20、 N40-30-30、 N80-0-20+S、 N60-20-20+S、 N40-30-30+S和CK。水稻收获期采集代表性样品考察水稻产量结构性状, 同时测定水稻籽粒和秸秆产量, 分析籽粒和秸秆氮素含量, 计算水稻氮素吸收量和氮肥利用效率。【结果】基肥-分蘖肥-穗肥施用比例60-20-20的处理水稻籽粒产量最高, 两年试验较不施分蘖肥的对照分别增产9.4%~12.9%和7.4%~8.9%。实施小麦秸秆直接还田后, 水稻籽粒产量较不施秸秆的对照分别提高10.2%~23.4%和0.8%~5.5%。不施秸秆条件下, 基-蘖-穗肥施用比例60-20-20的处理水稻籽粒含氮量最高, 较不施氮的对照提高11.3%, 而秸秆含N量随中后期追肥比例的加大而提高。秸秆还田条件下, 氮肥后移能明显提高水稻籽粒和秸秆含氮量。水稻籽粒氮素吸收量, 基-蘖-穗肥比例60-20-20处理最多, 2011年较对照N80-0-20分别增加13.7%和24.8%, 2012年提高14.5%和9.2%; 秸秆氮素积累量则随中后期追肥用量的增加而增多, 基-蘖-穗肥比例40-30-30处理最多。不施秸秆条件下, 基-蘖-穗肥比例60-20-20的处理氮素干物质生产效率、 氮素稻谷生产效率、 氮收获指数均最高, 百公斤籽粒吸氮量最低。秸秆还田条件下, 氮素干物质生产效率和氮素稻谷生产效率均随中后期追肥量的增加而下降, 而百公斤籽粒吸氮量则最高。氮素农学效率、 氮肥回收利用率和偏生产力也是60-20-20比例的处理最高, 较对照N80-0-20农学效率分别提高4.90和2.44 kg籽粒/kg N, 氮肥利用率提高7.82和21.29个百分点, 偏生产力提高4.90和2.44个百分点。【结论】综合水稻产量、 氮素吸收量以及氮肥利用效率, 安徽省江淮丘陵低产白土地区, 小麦秸秆直接还田条件下, 单季中稻氮肥的基肥-分蘖肥-穗肥施用比例, 以60-20-20运筹方式较为适宜。     

Optimizing nitrogen management strategy under wheat straw incorporation for higher rice production and nitrogen use efficiency

Nitrogen (N) application plays an important role in rice production. Limited attention has already been paid to optimizing N fertilizer management strategy for higher grain yield and nitrogen use efficiency (NUE) of rice with crop residue incorporation. Field experiments were conducted with the objective to determine the response of several N application methods to rice production and to evaluate their NUE. Three N fertilizer application methods, i.e., local farmers' N fertilizer practice (FNP), modified farmers' N fertilizer practice (MNP), and increased the amount of N fertilizer practice (INP), were adopted with zero N application as control (CK). The results showed that, compared with that under FNP, grain yield was significantly higher under MFP, owing to signficantly enhanced total spikelets as a result of more panicles per unit area. Relative to FNP, MNP markedly increased nitrogen agronomic efficiency (AE_N), nitrogen recovery efficiency (RE_N), nitrogen physiological efficiency (PE_N) and nitrogen partial factor productivity (PFP_N), but AE_N, PE_N and PFP_N of INP were significantly lower. Further analysis showed that the number of tiller, leaf area index, aboveground biomass, SPAD value, plant N content and N uptake at the early vegetative stage were improved significantly under MNP compared to those under FNP, contributing to higher total aboveground biomass and total N uptake.     

Integrated rice management simultaneously improves rice yield and nitrogen use efficiency in various paddy fields

The hilly area of Southwest China is a typical rice production area which is limited by seasonal droughts and low temperature in the early rice growth period. A field experiment was conducted on three typical paddy fields (low-lying paddy field, medium-elevation paddy field, and upland paddy field) in this region. Nitrogen (N) treatment (180 kg N ha^-1 year^-1) was compared to a control treatment (0 kg N ha^-1 year^-1) to evaluate the effects of integrated rice management (IRM) on rice growth, grain yield, and N utilization. Integrated rice management integrated raised beds containing plastic mulch, furrow irrigation, and triangular transplanting. In comparison to traditional rice management (TRM), IRM promoted rice tiller development, with 7-13 more tillers per cluster at the maximum tillering stage and 1-6 more tillers per cluster at the end of tillering stage. Integrated rice management significantly increased the rice aboveground biomass by 34.4%-109.0% in different growth periods and the aboveground N uptake by 25.3%-159.0%. Number of productive tillers significantly increased by 33.0%, resulting in a 33.0% increase in grain yield and 8.0% improvement of N use efficiency (NUE). Grain yields were significantly increased in all three paddy fields assessed, with IRM being the most important factor for grain yield and productive tiller development. Effects of paddy field type and N level on N uptake by aboveground plants were reflected in the rice reproductive growth period, with the effects of IRM more striking due to the dry climate conditions. In conclusion, IRM simultaneously improved rice yield and NUE, presenting a valuable rice management technique in the paddy fields assessed.     

高、中、低产田水稻适宜施氮量和氮肥利用率的研究

为探明不同施氮水平对湖北省高、 中、 低产田水稻产量和氮肥利用率的影响。选用水稻品种两优培九为供试品种,采用大田小区试验,探索不同地力水平（高、 中、 低）下稻田的最佳施氮量,考察不同施氮水平对不同地力水平水稻产量及产量构成因素、 氮肥贡献率、 土壤氮素依存率和氮肥利用率的影响规律。结果表明, 在2011年大田试验中,高产田和中产田都在施氮量为N 180 kg/hm2 的处理中获得最高产量,分别比CK增产10为探明不同施氮水平对湖北省高、 中、 低产田水稻产量和氮肥利用率的影响。选用水稻品种两优培九为供试品种,采用大田小区试验,探索不同地力水平（高、 中、 低）下稻田的最佳施氮量,考察不同施氮水平对不同地力水平水稻产量及产量构成因素、 氮肥贡献率、 土壤氮素依存率和氮肥利用率的影响规律。结果表明, 在2011年大田试验中,高产田和中产田都在施氮量为N 180 kg/hm2 的处理中获得最高产量,分别比CK增产10.70%、 27.23%;而低产田则是在施氮为N 240 kg/hm2处理中产量达到最大,比CK增产44.70%。在2012年大田试验中,高产田、 低产田均在施氮为N 180 kg/hm2 时达到最高产量,分别比CK增产12.43%、 74.19%;而中产田在施氮处理为N 240 kg/hm2 时达到最大,比CK增产28.80%。在一定范围内,施氮量越高,氮肥农学利用率和氮肥生理利用率越高,偏生产力越低。综合产量、 产量构成因子以及氮肥利用率得出高产田与中产田适宜施氮量为N 120~180 kg/hm2,低产田适宜施氮量为N 180~240 kg/hm2。适宜施氮量上低产田中产田高产田。     

Characterization and implication of phytolith-associated potassium in rice straw and paddy soils

Rice straw contains up to 2.3% K in dry matter, including potassium (K) subcompartmented in phytoliths, complex siliceous structures formed in plant tissue via precipitation of Si. Rice straw is usually returned to the soil as a conventional practice to sustain soil nutrients, and therefore, the K pool accompanied with rice straw phytoliths is also cycled. Based on phytoliths obtained by ashing of rice straw at 400 °C and dissolution experiments using batch extraction in combination with physical separation of phytoliths by heavy liquid, this study evaluated the phytolith K(phytK) pool in rice straw and aged phytoliths in paddy soils. Entrapped organic matter containing K within phytolith silica cells was visualized by X-ray tomographic microscopy, and releases of this phytK pool accompanying phytolith dissolution were quantified. A 1% Na₂CO₃ solution, which has been commonly used to extract amorphous Si and to quantify soil phytoliths, showed obvious responses for K derived from phytolith dissolution, indicating that the Na₂CO₃ method can be developed for measurement of phytK. In 13 soil samples, Na₂CO₃-dissolvable K content assignable to phytK was 0.55 ± 0.39 g kg^?1 in the puddled horizon, suggesting the phytK pool is of high significance for the management of K in paddy soils.     

Soil organic carbon and phosphorus availability regulate abundance of culturable phosphate-solubilizing bacteria in paddy fields

Low availability of phosphorus(P) is a major constraint for optimal crop production, as P is mostly present in its insoluble form in soil. Therefore,phosphate-solubilizing bacteria(PSB) from paddy field soils of the Indo-Gangetic Plain, India were isolated, and their abundance was attempted to be correlated with the physicochemical characteristics of the soils. Ninety-four PSB were isolated on Pikovskaya's agar medium, and quantitative phosphate solubilization was evaluated using NBRIP medium. The isolates solubilized P up to a concentration of 1 006 μg mL~(-1) from tricalcium phosphate with the secretion of organic acids. These isolates were identified by 16 S rRNA gene sequence comparison, and they belonged to Gammaproteobacteria(56 isolates),Firmicutes(28 isolates), Actinobacteria(8 isolates), and Alphaproteobacteria(2 isolates). Phylogenetic analysis confirmed the identification by clustering the isolates in the clade of the respective reference organisms. The correlation analysis between PSB abundance and physicochemical characteristics revealed that the PSB population increased with increasing levels of soil organic carbon, insoluble P, K~+, and Mg~(2+). The promising PSB explored in this study can be further evaluated for their biofertilizer potential in the field and for their use as potent bio-inoculants.     

不同耕作模式下旱作玉米氮磷肥产量效应及水分利用效率

为了探讨不同耕作模式下氮磷肥施用量对旱作玉米产量及水分利用效率的影响,于2003－2008年在山西寿阳旱农试验站进行了免耕、少耕和传统耕作下氮磷肥用量（105、179和210 kg/hm2 N;N∶P2O5=1∶1）试验。6 a结果显示,该区推荐氮磷用量为105 kg/hm2,传统耕作模式下玉米平均产量和水分利用效率分别为5 234 kg/hm2和12.4 kg/(hm2·mm);少耕模式下玉米平均产量和水分利用效率分别达到5 751 kg/hm2和13.6 kg/(hm2·mm),较传统耕作提高9.9%和9.7%。而免耕模式下氮磷用量为179 kg/hm2时玉米平均产量和水分利用效率最高,分别为5 336 kg/hm2和13.2 kg/(hm2·mm),较传统耕作提高6.1%和9.7%。免耕模式下土壤保水效果最佳,干旱年增产作用尤为明显。3种耕作模式下玉米平均产量和水分利用效率以少耕为最高,免耕次之,传统耕作最低。     

长期不同施肥对红壤性水稻土磷素及水稻磷营养的影响

【目的】合理的土壤磷素管理对作物生产和环境保护具有重要意义。南方双季稻田土壤磷素特征及磷素吸收信息相对缺乏,本文利用江西省稻田土壤质量演变定位监测试验为平台,系统分析长期不同施肥措施下土壤全磷、磷活化系数及水稻磷素吸收量的变化特征和全磷与磷盈亏的响应关系等,为指导磷肥合理施用提供重要科学依据。【方法】从1984年开始在江西省南昌市进行长期定位试验,设置8个处理,分别为不施肥对照(CK),PK、NP、NK、NPK、70%化肥氮+30%有机肥氮(70F+30M)、50%化肥氮+50%有机肥氮(50F+50M)、30%化肥氮+70%有机肥氮(30F+70M)。早稻施用纯N、P2O5和K2O量分别为150、60和150 kg/hm^2,晚稻分别为180、60和150 kg/hm^2。早、晚稻施用的氮、磷、钾化肥均分别为尿素、过磷酸钙和氯化钾,有机肥分别为紫云英(N、P2O5、K2O含量分别为0.30%、0.08%、0.23%)和腐熟猪粪(N、P2O5、K2O含量分别为0.45%、0.19%、0.60%)。除30F+70M处理,其余处理均为等氮磷钾设计。于1984-2012年每年早、晚稻收获期采集秸秆和稻谷计产,并于晚稻收获后,测定土壤全磷和有效磷含量。分析土壤全磷、磷活化系数(PAC)及早、晚稻磷素吸收量随种植年限的变化规律,研究土壤全磷含量与磷累积盈亏的响应关系。【结果】经29年连续试验,NK处理土壤全磷含量以每年4.6 mg/kg的速度下降,而含磷化肥处理土壤全磷含量升高速率为3.3~19.4 mg/(kg·a)。有机无机配施处理(70F+30M、50F+50M和30F+70M)升高速率平均为16.1 mg/(kg·a),是施NPK肥处理的4.89倍。施磷土壤全磷含量平均增至1.07 g/kg (2010-2012平均值),较初始值提高了1.18倍。不施磷肥处理土壤磷活化系数(PCA)由试验初始的4.24%下降至2.5%左右,施磷肥处理则均显著升高,其中有机无机配施处理平均升高至8.51%,平均年升高速率是施NPK处理的2.89倍。早、晚稻磷素吸收量,施磷肥(PK、NP和NPK)和化肥配施有机肥处理(70F+30M、50F+50M和30F+70M)均显著高于CK,提高幅度分别为29.9%~124%和28.6%~103%,均衡施肥(NPK、70F+30M、50F+50M和30F+70M)磷素吸收量显著高于不均衡施肥(PK和NP)处理,前者平均分别较后两者提高了38.7%和32.9%。早、晚稻产量与磷素吸收量呈极显著线性正相关关系,每吸收磷(P) 1 kg,早稻和晚稻产量分别可提高115和106 kg/hm^2。不施肥(CK)条件下,土壤全磷变化与累积磷盈亏间无显著相关关系,施NK肥处理土壤中每亏缺磷100 kg/hm^2,土壤全磷含量降低6.0 mg/kg,施化学磷肥的3个处理,土壤中每盈余磷100 kg/hm^2,平均提高9.3 mg/kg,而3个有机–无机配施处理,土壤中每盈余磷100 kg/hm^2,平均增加63.3 mg/kg,是无机磷肥的6.78倍。【结论】无论是单施化学磷肥,还是有机无机配施均有效提高土壤全磷含量及磷活化系数,且在等磷量投入条件下,有机无机配施较单施化肥的效果更优。建议减少中国南部红壤性稻田土壤的总磷输入量和提高有机肥施用比例,以改善粮食生产和保护环境。