杨树长期育种中遗传测定效率理论分析

The major goal for long-term poplar breeding can be formulated as maximizing annual progress in Group Merit Gain at a given annual budget （GMG/Y＊）. To evaluate different breeding scenarios, a deterministic simulator BREEDING CYCLE ANALYZER covering the most important aspects （gain, cost, time, technique, and gene diversity） of a full breeding cycle was used. The breeding strategies considered was based on pairwise crossing of the selected breeding population and balanced within family selection for the next breeding population. A main scenario and a number of alternative scenarios within these constraints were evaluated using estimates of the best available inputs for poplars. In focus was a comparison between three different testing scenarios for selecting the parents mated to create future breeding generations, thus selecting based on phenotype, clone test or progeny test. For the main scenario, the highest GMG/Y, and the optimal selection age for clone, phenotype and progeny strategies were 0.7480 %, 0.6989% and 0.4675%; 7, 6, and 11 years respectively. Clone test was best except when heritability was high, plant price was high or total budget was low; phenotype strategy was the second except for the case of extremely low narrow-sense heritability, for which the progeny strategy was a little more efficient than phenotype strategy. GMG/Y was markedly affected by narrow-sense heritability, additive variance at mature age, rotation age, plant-dependent cost, total budget and the time needed to produce the test plants, while diversity loss and recombination cost had rather weak effect on GMG/Y. Short rotation age and cheap testing cost favoured all three testing strategies. Comparably short rotation age, low plant-dependent cost and high total budget seem to promote early selection for progeny strategy.     

Efficiency of breeding strategy where grandparents — but not parents — contribute equally to the breeding population

? The objective was to optimise the strategy for maintaining the genetic balance in long-term breeding where the grandparents contribute equally to the breeding population, but the parents do not.

? The annual genetic progress under a budget constraint was compared for a number of scenarios. The factors considered were: genetic parameters, recruitment population size, mating number per grandparent, cost of plants and parents, selection age and time components. Phenotypic selection forward was assumed. Using more parents than grandparents affects the testing population only, thus, gene diversity loss and breeding population size remain constant.

? Using larger number of parents than grandparents was a superior strategy at all scenarios tested. The strategy with 6 parents per grandparent and 1% of the tested plants selected on their phenotype at age 15 for further mating is recommendable. The strategy to choose 6 parents per grandparent was robust over a heritability range 0.05 to 0.2. At a higher heritability, the optimum number of parents per grandparent was 2 to 4.

? Using larger number of parents than grandparents has the potential to improve annual genetic gain in the magnitude of 50% compared to the strategies currently used for Scots pine in Sweden.

     

Balancing seed yield and breeding value in clonal seed orchards

Seed orchards should produce seeds that are both abundant and of high genetic value. This study suggests methods to achieve such a compromise and study their efficiency. The methods were applied on data obtained from 41 seed orchard clones of Scots pine from mid-Sweden. The value of the seed orchard crop was set as a function of its breeding value, the amount of seeds produced and their gene diversity, measured as the effective number of clones. The proportion of ramets of different clones that maximized this value was regarded as the optimum for deployment of the clones in a seed orchard. The results were compared with truncation selection for breeding value, truncation selection for clone benefit (the product of seed production and breeding value) and linear deployment (where ramets are deployed linearly in relation to breeding value). The influence of two parameters was studied: the relative importance of breeding value for seed value and the size of the penalty for reducing the value of the seed crop with respect to lost gene diversity. The conventional wisdom is to select the clones with the highest breeding values, but that turned out to be the most inferior alternative studied. Clone benefit truncation provided a good approximation to optimal benefit for cases, where the effective number was low and dependence of breeding value limited.     

Early selection in eucalypt breeding in Australia – optimum selection age to minimise the total cost of kraft pulp production

Optimum ages for early combined-trait selection were derived for a breeding objective of reducing the total cost of unbleached eucalypt kraft pulp, assuming typical heritabilities and age-age genetic correlations for basic density and growth. The optimum biological selection age, the selection age where average annual gain is maximised, was four years for an assumed rotation age of 15 years. The optimum economic selection age, the selection age where the present value of all future gains is maximised, was three years. These conclusions were relatively robust to changes in assumptions used in analysis.     

Genomic selection in forest tree breeding: the concept and an outlook to the future

Using large numbers of DNA markers to predict genetic merit [genomic selection (GS)] is a new frontier in plant and animal breeding programs. GS is now routinely used to select superior bulls in dairy cattle breeding. In forest trees, a few empirical proof of-concept studies suggest that GS could be successful. However, application of GS in forest tree breeding is still in its infancy. The major hurdle is lack of high throughput genotyping platforms for trees, and the high genotyping costs, though, the cost of genotyping will likely decrease in the future. There has been a growing interest in GS among tree breeders, forest geneticists, and tree improvement managers. A broad overview of pedigree reconstruction and GS is presented. Underlying reasons for failures of marker-assisted selection were summarized and compared with GS. Challenges of GS in forest tree breeding and the outlook for the future are discussed, and a GS plan for a cloned loblolly pine breeding population is presented. This review is intended for tree breeders, forest managers, scientist and students who are not necessarily familiar with genomic or quantitative genetics jargon.     

Breeding without breeding: selection using the genomic best linear unbiased predictor method (GBLUP)

We demonstrate, using height data from a clonal trial, how the genomic best linear unbiased predictor method (GBLUP) is ideal for determining future breeding potential in situations (either in plantations or wild stands) where high mortality due to biotic or abiotic factors has occurred. The method is effective because it does not require the development of structured pedigree or classical progeny testing, rather it uses DNA fingerprinting to determine the genealogical relationship among individuals. The resulting genetic network is known as the realized relationship matrix, which in turn is used in classical quantitative genetics analyses to determine the genetic worth of all fingerprinted individuals. Selection of desirable individuals among the surviving population is aimed at maximizing genetic diversity even when the original genetic source is unknown. This is accomplished by determining the number of founder genome equivalents which can be used to estimate the inbreeding effective population size. During the selection phase, genetic diversity can be balanced against genetic gain so that diversity is maximized while gain for any particular attribute is optimized.     

森林道路对山雀类利用人工鸟巢的影响

于2002 年11月至2003年6 月间, 在韩国安养首尔大学冠岳植物园(37°25'05"N,126°56' 85"E)阔叶林、针叶林和针阔混交林内研究了森林道路对山雀类利用人工鸟巢的影响。对在人工巢箱中繁殖的山雀类,杂色山雀(Parus varius)、沼泽山雀( P. palustris)和大山雀(P. major)的繁殖对的数量、巢位、窝大小、卵重及卵大小进行了调查。调查结果显示,在 3 个研究区,杂色山雀在森林内部(距离道路 75–150m)的繁殖对数量,比森林边缘(距离道路 0–75 m)多,其窝大小、卵重等尺寸也明显比森林边缘高,但沼泽山雀基本不受森林道路的影响。在人类活动频繁的地区人工巢箱为洞穴鸟类提供良好的繁殖条件。人工鸟巢的设置将有利于这些鸟类的保护和管理。表 3 参 16。     

Optimum lifetime for Swedish <Emphasis Type="Italic">Picea abies</Emphasis> seed orchards

The optimum lifetime of Picea abies seed orchards in Sweden was calculated using a model which considers changes in seed yield over time, annual progress of genetic gain, establishment, management and seed costs, and both the monetary and genetic value of the seeds produced. The longer the seed orchard is active, the more genetically outdated the produced seeds will become, and thus their value will decline with time. A main scenario was constructed using the best available estimates for Swedish Norway spruce and was compared to various alternatives reflecting likely variations. The optimal lifetimes in all of these scenarios was in the order of 40 years. Sensitivity analyses showed that use of orchards for slightly more or less than their optimal lifetimes does not lead to large losses. A more effective tree breeding program could slightly reduce the optimal lifetimes. The optimal lifetime depends strongly on the relationship between monetary value and genetic gain. Thus, in cases where genetic progress is sufficiently rapid, the optimal lifetime may be less than 30 years.     

Genetic characterisation of a Eucalyptus nitens base breeding population in South Africa

The measurement and statistical analysis of data from eight Eucalyptus nitens trials, established in the summer rainfall forestry region of South Africa during the 1980s and 1990s, have enabled the characterisation of the Institute for Commercial Forestry Research's breeding population. Provenance testing showed that the more northerly New South Wales (Australia) Eucalyptus nitens provenances of Barren Mountain and Barrington Tops are distinctly better suited to the summer rainfall areas of South Africa than the southern New South Wales provenances and the Victorian provenance, Penny Saddle. Generally, the species was not badly affected by Coniothyrium canker. High type B genetic correlations for all site pairs, except one comparison, ranged from 0.75 to 0.99 for diameter at breast height (dbh), indicating very little or no genotype × environment interaction for dbh for the genotypes tested in this study. Narrow-sense heritability coefficients ranged from 0.01 to 0.34, indicating that the species generally exhibited sufficient breeding opportunity for improvement of diameter growth. High genetic correlations of greater than 0.90 between diameter measurements at 52 to 62 months after establishment and diameter measurements at 94 or 113 months were found, indicating that selections can be reliably made at five or six years. Predicted genetic gains were highest in the trials at Goedehoop and Arthur's Seat, with increases in dbh of 3.07 cm (17.1%) and 3.17 cm (20.7%), respectively, at full rotation.     

江苏盐城黑嘴鸥繁殖期不同阶段行为时间分配及活动规律

1999— 2 0 0 2年夏季对江苏盐城黑嘴鸥繁殖期不同阶段行为时间分配及活动规律进行了定量研究。结果表明 :繁殖前期黑嘴鸥各种行为具有较强的规律性 ,其出现频率依次为 :取食 39 36 % ,休整 37 95 % ,运动 9 4 8% ,警戒 8 30 % ,饮水 5 0 3% ;繁殖后期黑嘴鸥成鸟的各种行为不具明显的规律性 ,其出现的频率依次为取食 4 2 5 3% ,休整 1 4 2 1 % ,运动 1 7 6 5 % ,警戒 1 4 5 2 % ,饮水 1 0 93%。繁殖后期运动和警戒行为所花费的时间比繁殖前期显著增加 (P <0 0 0 1 ) ,休整行为显著减少 (P <0 0 0 1 ) ,导致这种差异性主要是取食地点的变化和后期的育雏行为。黑嘴鸥雌雄抱孵时间比为 1 2 4∶1。抱孵时间随孵化天数的增加而相应延长 ,一次抱孵时间最长可达到 2 0 1min。     

花桥早熟板栗实生选种研究

1996~2005年,我们对花桥早熟板栗开展了实生选种研究,初步选育出花桥1号和花桥2号两个优良早熟新品种。研究结果表明:花桥1号和花桥2号两个新品种确实具有成熟期早、果粒大、抗性强、栽培管理容易、丰产性好、商品价值高等优良性状,在湘中丘岗山地具有广阔的开发前景,属地方名特优新品种。     

Variations in growth, survival and carbon isotope composition (delta(13)C) among Pinus pinaster populations of different geographic origins

To evaluate differences in growth and adaptability of maritime pine (Pinus pinaster Ait.), we studied growth, polycyclism, needle tissue carbon isotope composition (delta(13)C) as an estimate of water-use efficiency (WUE) and survival of seven populations at 10 years of age growing in a performance trial at a provenance test site in Escaroupim, Portugal. Six populations were from relatively high rainfall sites in Portugal and southwestern France (Atlantic group), and one population was from a more arid Mediterranean site in Spain. There were significant differences between some populations in total height, diameter at breast height, delta(13)C of bulk needle tissue, polycyclism and survival. A population from central Portugal (Leiria, on the Atlantic coast) was the tallest and had the lowest delta(13)C. Overall, the variation in delta(13)C was better explained by the mean minimum temperatures of the coldest month than by annual precipitation at the place of origin. Analyses of the relationships between delta(13)C and growth or survival revealed a distinct pattern for the Mediterranean population, with low delta(13)C (and WUE) associated with the lowest growth potential and reduced survival. There were significant negative correlations between delta(13)C and height or survival in the Atlantic group. Variation in polycyclism was correlated with annual precipitation at the place of origin. Some Atlantic populations maintained a high growth potential while experiencing moderate water stress. A detailed knowledge of the relationships between growth, survival and delta(13)C in contrasting environments will enhance our ability to select populations for forestry or conservation.     

A comparison of deterministically predicted genetic gains with those realised in a South African Eucalyptus grandis breeding program

Tree breeders attempt to predict the genetic gains that are likely to be achieved through selection and breeding of new generations, using stochastic or deterministic modelling. There are many factors that may cause a discrepancy between the predicted and realised genetic gains. Often the predictions for genetic gains are based on single trait selection, whereas in reality the breeding tends to be multitrait in nature. The violation of Hardy-Weinberg conditions, assumptions regarding outcrossing and relatedness, assumptions regarding the effect of the interaction between the environment and the genotype, and numerous possible errors in the process of breeding, all could result in unexpected discrepancies between the realised and predicted genetic gains. A series of genetic gains trials containing representatives of three generations of Eucalyptus grandis selections were compared with the view to verifying the effectiveness of the E. grandis breeding program. Genetic gains of the F₃ (third generation of pedigreed progeny) over the F₂ generation (second generation of pedigreed progeny) were 15% for tree growth (volume). A comparison between F₂ and P₀ revealed an improvement of between 20% and 33% for growth. This exercise highlighted complexities of modelling the predicted genetic gains of assimilated genetic breeding trials. The predictions of genetic gains did deviate (in both directions) from those realised, although these deviations may be explained as functions of imperfect modelling. On average, however, the predicted genetic gains for tree volume over three generations was 13% between generations, whereas the average realised genetic gain in the genetic gains trial was 14%. It is therefore assumed that the E. grandis breeding population is indeed performing as expected, following classical tree breeding assumptions.     

红角鸮种群数量及繁殖生态的调查研究

红角鸮在山西省内分布广泛,笔者于2008年至2010年在阳圪台林场对红角鸮的迁徙、种群数量、繁殖特性、栖息地和食物组成等进行了生态调查,结果表明,红角鸮在山西省的迁徙特点是每年最早迁入的日期差异为2 d~4 d,最晚迁离的日期差异为1 d~4 d,居留期为162 d~167 d,间隔期为198 d~204 d;红角鸮繁殖季前的种群数量为0.75只/km2,繁殖季后为1.08只/km2;鼠类、两爬类、昆虫类等为其主要食物。     

云南本地野鸡与美国七彩山鸡杂交繁殖技术初探

近几年野生动物养殖逐渐有了市场,珍禽野味以其独特的风味,较高的食用价值、药用价值、观赏价值,深受市场青睐。但是,野生野鸡受国家法律保护,加之自然界存量极少,因此大力发展人工养殖野鸡相关产业,对林业产业的发展将会有一定的推动作用。美国七彩山鸡因其长期人工驯养,品种逐渐退化,急需引进野生品种基因作为外源基因,达到优化品种的目的。本文针对云南本地野鸡与美国七彩山鸡的杂交繁殖研究,从技术上来论证以云南本地野鸡作为美国七彩山鸡品种改良外源基因来源的可行性。     

Genetic parameters and comparison between native and local landraces of Eucalyptus globulus Labill. ssp. globulus growing in the central highlands of Colombia

Aims

The objective of this study was to compare the merit of the Colombian landrace relative to the various Australian native races of Eucalyptus globulus Labill. ssp. globulus and study the genetic control of key traits such as growth, wood density, and leaf phase change in the unique conditions of the Colombian highlands.

Methods

The genetic study was based on open-pollinated families from native Australian and Colombian landrace origin, tested across four trials spanning two generations of breeding. A multisite mixed linear model with genetic groups was fitted to the data to estimate race merit and the variance and covariances between traits, ages, and sites.

Results

Race effects for growth were small and only significant at the older site. In contrast, races differ significantly for height to phase change and density. The Colombian landrace and South and NE Tasmania races changed leaf type at a higher tree height. King Island and Recherche Bay had low density values, whereas the Colombian landrace had the highest. Heritability was moderate for growth traits (between 0.09 and 0.40), high for height to phase change (between 0.42 and 0.69), and moderate for wood density (0.28). The genetic correlation between growth and height to phase change was in general positive, but variable across sites. There was no correlation between growth and density. Despite large differences in growth between trials, pairwise genetic correlations suggest that genotype-by-site interaction is negligible. However, there was a poor correspondence between first-generation (ex-native stands) and second-generation families (ex-multi-provenance progeny trial). This suggests that breeding value estimates based on native open-pollinated material are unreliable.

Conclusion

Results confirm negligible race differences for growth, but not for wood density. Future breeding efforts should include the various genetic backgrounds including the Colombian landrace which constitutes a very important source, especially because of its high density. Despite a low Genotype by Environment interaction, the poor genetic correspondence between the first- and second-generation trials indicates that selections for growth should be based on information coming mainly from the advanced-generation trials.     

Norway spruce emblings as cutting donors for tree breeding and production

In the Nordic countries, Norway spruce (Picea abies) is a major species in tree breeding. In order to facilitate breeding work and availability of highly bred forest regeneration material, the time required for breeding and implementation of results should be shortened. This could be done by accelerating production of clonal material for field testing, and possibly for planting stock, by combining production of rooted cuttings with somatic embryogenesis (SE). This would allow efficient production of numerous plants of the same genotype, with equal age and propagation history between genotypes. In the present work, we studied the rooting potential of cuttings from Norway spruce emblings. Altogether 36 clones from 12 families representing elite breeding materials and ornamental forms were examined under different rooting conditions (container type and rooting media) in 2015 and 2016. Our results show that Norway spruce emblings are good donors for cuttings. Best combination (peat–vermiculite mixture and Plantek 81f containers) resulted in 91% rooting, variation among the tested clones of elite breeding materials being 55–100% per treatment. The rooting variation between families is acceptable for breeding purposes. High rooting (87–96%) of ornamental forms indicates propagation potential with the combination of SE and rooted cuttings.     

Phenotypic plasticity and climatic adaptation in an Atlantic maritime pine breeding population

Context

Pinus pinaster Ait. is found in the Iberian Peninsula under Mediterranean and Atlantic conditions. Both climates encounter each other in Galicia (NW Spain), where two bioclimatic regions can be differentiated: coastal and inland. A breeding program was launched in the coastal area, with two breeding and deployment areas delimited.

Aims

We analyse plasticity patterns across regions in a coastal breeding population to assess the suitability of current breeding areas and how genetic material will likely respond to future climate.

Methods

Total height at ages 3 and 8?years was assessed in 16 trials established along the coast and in inner Galicia. Clustering of environments with similar genotypic performance, family sensitivities to climatic factors and stability analyses were performed.

Results

Sizeable genetic variation in plasticity was found among families, and crossover genotype-by-environment interactions were detected within and between regions. It was unfeasible to regionalize Galicia into alternative areas of stable genotypic performance. Only the cold regime was found to noticeably underlie the array of phenotypic responses to changing environmental conditions.

Conclusions

Results suggest that previous delimitation in two breeding areas is pointless and indicate reduced effects of a changing climate towards Mediterranean conditions on decreasing population fitness.     

Predicting individual breeding values and making forward selections from open-pollinated progeny test trials for seed orchard establishment of interior Lodgepole Pine (<Emphasis Type="Italic">Pinus contorta</Emphasis> ssp. <Emphasis Type="Italic">latifolia</Emphasis>) in British Columbia

Ten-year height data from three open-pollinated lodgepole pine progeny test trials were analysed in order to select individuals for seed orchard establishment in the low and high elevation seed-planning units (TOLO and TOHI) of the new Thompson Okanagan seed planning-zone in interior British Columbia, Canada. The total test population had about 35,000 individuals from 362 families of 9 provenances and was divided into two portions, each serving as the base population for its appropriate seed-planning unit. For each population, various genetic parameters were estimated and individual breeding values were predicted. Combined-site estimates of individual, family, and within-family heritability ranged from 0.23 to 0.38, 0.77 to 0.84, and 0.17 to 0.29, respectively. Estimated coefficients of type-b genetic correlation were greater than 0.81, indicating little G × E interaction in this region. Provenance effects were large. Larch Hills and Inonoaklin River were the two superior provenances with an expected gain of 13% and 10% in stem volume at rotation age of 60 years in TOLO. Individual breeding values was not uniformly distributed among provenances and the top 100 individuals originated from 20 families in TOLO and 32 families in TOHI. Co-ancestry among these individuals should not be a serious concern in seed orchard establishment as the predicted genetic loss due to excessive inbreeding is only 1.06% in TOLO and 0.6% in TOHI. The status numbers of the two prospective seed orchards are 32 and 44, respectively, and both are well above the required minimum of 10 in British Columbia. Using unrelated clones for seed orchard establishment may result in reduced optimum genetic gain and thus is not a favourable option.     

河北平山鸳鸯(Aix galericulata)繁殖种群亟待保护

自2003年来在河北平山蛟潭庄镇-合河口乡一带发现鸳鸯繁殖种群后,其种群数量逐年增加表明此地具有适宜鸳鸯的繁殖生境（如筑巢地、觅食地等）,但附近较多的人类活动给这个正在增长的繁殖种群带来了潜在风险。因此,维持此繁殖种群的稳定亟待制定合理保护策略和增强当地公众保护意识。