Effect of a mixed herb-enriched diet on the innate immune response and disease resistance of Paralichthys olivaceus against Philasterides dicentrarchi infection

We investigated the effect of a mixed herb-enriched diet obtained from pomegranate Punica granatum, Dalmatian chrysanthemum Chrysanthemum cinerariaefolium, and mastic-leaved prickly-ash Zanthoxylum schinifolium on innate immune mechanisms (e.g., phagocytosis activity, respiratory burst activity, alternative complement activity, lysozyme activity, and disease resistance) of olive flounder Paralichthys olivaceus against the scuticociliate Philasterides dicentrarchi. All experimental groups were challenged with P. dicentrarchi (1 x 10(5) ciliates/mL) through intraperitoneal administration of the pathogen (50 microL) on day 1. On day 7, the infected groups were fed 0, 5, 50, and 100 mg/kg of the enriched diets. The innate immune parameters, cumulative mortality, and relative percent survival (RPS) were assessed at weeks 1, 2, 3, and 4. Administration of 50 or 100 mg/kg of the herbal-enriched diet enhanced immunity throughout the experimental period. However, at the 5-mg/kg dosage, the enriched diet did not enhance the innate immune estimates at any time. At doses of 50 and 100 mg/kg, administration of the diet preceding the challenge with P. dicentrarchi decreased the percentage cumulative mortality in the experimental groups and thereby increased RPS values. This study reports that administration of 50 or 100 mg/kg mixed herbal-enriched diet could positively influence the innate immune response to P. dicentrarchi and enhance the health status of olive flounder with respect to this microbe.     

Korean mistletoe enriched diet enhances innate immune response in kelp grouper, Epinephelus bruneus against Philasterides dicentrarchi

The present study investigated the immunostimulatory effect of Korean mistletoe Viscum album extract (KM-E) on innate immune response in kelp grouper Epinephelus bruneus against Philasterides dicentrarchi. Kelp grouper were divided into four groups of 25 each and fed with 0 (control), 0.5, 1.0, and 2.0% enriched diets with Korean mistletoe extract (KM-E). After feeding for 30 days, the fish were injected intraperitoneally (i.p.) with 100 μl of P. dicentrarchi (4.2 × 10(7)ciliates/ml) to study the immune responses at weeks 1, 2, and 4. The respiratory burst activity did not significantly enhance when fed with 0.5% and 1.0% supplementation diets on week 1 when compared to control diet. On weeks 2 and 4, the respiratory burst activity significantly increased with 1.0% and 2.0% diets. The phagocytic activity significantly enhanced with 1.0% and 2.0% diets, but not with 0.5% diet at any time. When fed with 1.0% KM-E-diet the lysozyme activity did not significantly vary at any week whereas with 1.0% and 2.0% diets it was significantly enhanced. The total protein level significantly increased with 1.0% and 2.0% KM-E-diets from weeks 1 to 4 as compared to control. The present study suggests that 1.0% or 2.0% KM-E-supplementation diet positively enhances the innate immune response in E. bruneus against P. dicentrarchi infection.     

Effect of dietary iron content on hematologic and other measures of iron adequacy in dogs

Eighteen 9- to 10-week old Beagles were fed casein-based diets (4,710 kcal of metabolizable energy/kg of body weight) containing either 12, 80, or 160 mg of iron/kg of diet. Growth and feed consumption were monitored throughout the 47-day study. Hematocrit (Hct), hemoglobin (Hb) concentration, mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), RBC numbers, erythrocyte protoporphyrin (EP) concentration, serum iron concentration, serum total iron-binding capacity (TIBC), and serum ferritin concentration were determined weekly. Growth rate and feed efficiency were not significantly influenced by dietary iron content. At 14 days, Hb concentration, Hct, MCV, MCH, RBC numbers, and serum iron concentration were significantly (P less than 0.05) lower in dogs fed the 12 mg/kg diet, and remained significantly low for the remainder of the study. Erythrocyte protoporphyrin concentration increased significantly (P less than 0.05) by 14 days in dogs fed the basal diet, and remained significantly high relative to that in dogs of the other dietary groups for the remainder of the study. Serum ferritin concentration decreased in dogs of the group fed the basal diet, with a significant (P less than 0.05) difference beyond day 42. Differences in Hct, MCH, MCV, or hemoglobin, serum iron, serum ferritin, or EP concentration were not found between groups fed 80 and 160 mg of iron/kg of diet. Liver nonheme iron content was significantly (P less than 0.05) affected by dietary iron content.     

果寡糖对固始鸡血液生理指标的影响

本试验旨在研究不同添加剂量的果寡糖对固始鸡血液生理指标的影响,并确定果寡糖的最佳添加剂量。选用1日龄固始鸡公雏150只,随机分成5个处理组,1个为对照组,其余4个为试验组,对照组不加果寡糖,其他4个组的果寡糖添加水平分别为3、5、7mg/kg和9mg/kg。测定红细胞压积(PCV)、血红蛋白(Hb)含量、红细胞数量、白细胞数量、平均红细胞体积(MCV)、平均红细胞血红蛋白含量(MCH)和平均红细胞血红蛋白浓度(MCHC)等指标。试验结果表明:1)21和42日龄时,果寡糖试验组的红细胞压积、血红蛋白含量、红细胞数量和白细胞数量均显著高于对照组(P<0.05);与对照组相比,试验组的MCH和MCHC均略有升高,并且可以显著提高MCHC(P<0.05);2)日粮中添加5mg/kg果寡糖对提高红细胞压积、血红蛋白含量、红细胞数量、白细胞数量、MCH和MCHC具有最佳效果。     

铁对1~4周龄五龙鹅生产性能、造血功能和铁代谢的影响

本试验旨在研究饲粮铁添加水平对1~4周龄五龙鹅生产性能、造血功能和铁代谢的影响,以确定鹅饲粮铁的适宜添加水平。试验选用1日龄五龙鹅360只,随机分为6个组,每个组6个重复,每个重复10只。Ⅰ组为对照组,饲喂基础饲粮,Ⅱ~Ⅴ组分别饲喂在基础饲粮中添加40、80、120、160、200 mg/kg铁的试验饲粮。试验期4周。结果表明,与对照组相比:1)饲粮中添加80~120 mg/kg铁能显著或极显著增加五龙鹅的体重和平均日增重(P0.05或P0.01),显著降低料重比(P0.05);2)饲粮中添加80~120 mg/kg铁显著提高了鹅的屠宰率、半净膛率和腿肌率(P0.05);3)饲粮中添加80 mg/kg铁显著提高了鹅血液的平均红细胞体积和平均红细胞血红蛋白含量(P0.05),饲粮中添加80~200 mg/kg铁显著提高了鹅血液的红细胞数和血红蛋白浓度(P0.05),饲粮中添加120~200 mg/kg铁显著或极显著提高了鹅血液的红细胞压积(P0.05或P0.01);4)饲粮中添加160~200 mg/kg铁显著降低了鹅血清中总铁结合力(P0.05),显著提高鹅血清中转铁蛋白饱和度(P0.05);饲粮中添加120 mg/kg铁显著降低了鹅血清中尿素氮和肌酐含量(P0.05),饲粮中添加80 mg/kg铁显著降低了鹅血清中尿酸含量(P0.05)。由此可见,以最佳生长性能为目标,1~4周龄五龙鹅饲粮中铁的适宜添加水平为99.56~116.91 mg/kg。     

Effect of the mycotoxin aurofusarin on the antioxidant composition and fatty acid profile of quail eggs.

1. The effect of the mycotoxin aurofusarin on the antioxidant composition and fatty acid profile of quail eggs was investigated. 2. Thirty eight 45-d-old Japanese quails were divided into two groups (experimental and control, 15 females +4 males in each group) and were fed on a maize-soya diet balanced in all nutrients. The diet of the experimental quails was supplemented with aurofusarin at the level of 26.4 mg/kg feed in the form of Fusarium graminearum culture enriched with aurofusarin. At the beginning and after 2, 4 and 8 week supplementation periods, eggs were collected and analysed. After 8 weeks of supplementation, experimental quails were fed on unsupplemented diet during the next 4 weeks and eggs were collected after 2 and 4 weeks on such a diet and analysed. 3. Aurofusarin caused a significant (P<0.05) decrease in vitamins E, A, total carotenoid, lutein and zeaxanthin concentrations and significantly (P<0.05) increased egg yolk susceptibility to lipid peroxidation. During two weeks on the diet without aurofusarin the levels of carotenoids in the egg yolk returned to the initial level, vitamins A and E returned to the initial level during 4 weeks on the same unsupplemented diet. 4. Dietary supplementation with aurofusarin was associated with a significant (P<0.01) decrease in the docosahexaenoic acid proportion in the phospholipid, cholesteryl ester and free fatty acid fractions of the egg yolk. At the same time the proportion of linoleic acid in the phospholipid, free fatty acid and triacylglycerol fractions significantly (P<0.05) increased. 5. It is concluded that mycotoxin aurofusarin is detrimental to the nutritional quality of eggs.     

苜蓿多糖对夏季高温环境下蛋鸡生产性能及蛋品质的影响

本试验旨在研究夏季高温环境下在饲粮中添加苜蓿多糖对蛋鸡生产性能及蛋品质的影响。将540只156日龄海兰褐蛋鸡随机分成6组,每组6个重复,每个重复15只鸡。对照组饲喂基础饲粮,试验组分别饲喂在基础饲粮中添加250、500、1 000、2 000和4 000 mg/kg苜蓿多糖的试验饲粮。试验期为6周。结果显示:与对照组相比,1)饲粮中添加不同水平苜蓿多糖对平均蛋重、平均日采食量、不合格蛋率和死淘率均无显著影响(P0.05),而添加500、1 000和4 000 mg/kg苜蓿多糖显著提高了蛋鸡的产蛋率(P0.05),添加500、1 000、2 000和4 000 mg/kg苜蓿多糖显著降低了料蛋比(P0.05)。2)饲粮中添加不同水平苜蓿多糖对蛋白高度和哈夫单位均无显著影响(P0.05);250 mg/kg苜蓿多糖添加组第4和6周末的蛋黄颜色显著提高(P0.05);500 mg/kg苜蓿多糖添加组第6周末的蛋形指数显著降低(P0.05),第1、4和6周末的蛋黄颜色显著提高(P0.05);1 000 mg/kg苜蓿多糖添加组第4和6周末的蛋壳颜色显著加深(P0.05),第1、2和4周末的蛋壳强度和蛋壳厚度显著提高(P0.05),第1和6周末的蛋形指数显著降低(P0.05),第1、2、4和6周末的蛋黄颜色显著提高(P0.05);2 000 mg/kg苜蓿多糖添加组第1和4周末的蛋壳厚度显著提高(P0.05),第6周末的蛋形指数显著降低(P0.05),第1、4和6周末的蛋黄颜色显著提高(P0.05);4 000 mg/kg苜蓿多糖添加组第4周末的蛋壳颜色显著加深(P0.05)且蛋壳强度显著提高(P0.05),第1和4周末的蛋壳厚度显著提高(P0.05),第6周末的蛋形指数显著降低(P0.05),第1、4和6周末的蛋黄颜色显著提高(P0.05)。结果表明,夏季高温环境下,在饲粮中添加适量的苜蓿多糖可以有效缓解蛋鸡的热应激,提高蛋鸡的生产性能,改善蛋品质,且适宜添加量为1 000 mg/kg。     

Effect of different dietary levels of Yucca schidigera powder on the performance,blood parameters and egg yolk cholesterol of laying quails

A total of 135 laying quails (Coturnix coturnix japonica), 9 weeks old, were divided into three dietary treatment groups. Three replicates were assigned to each treatment group consisting of 15 birds per cage. The diet was supplemented with 0, 100 and 200 ppm Yucca schidigera powder and given ad libitum to the quails for a period of 14 weeks. Body weight, egg production, feed consumption and feed efficiency were not different due to dietary treatments among the groups. Increased egg weight was determined in the control group. Yucca powder supplementation decreased serum glucose, cholesterol triglyceride level in laying quails. Serum total protein concentration was not changed by dietary treatments but albumin level was decreased in quails fed 100 ppm yucca powder. Egg yolk cholesterol concentration was not significantly different among the groups but tended to decline (11.5%) as a result of yucca supplementation. Red Blood Cell (RBC) and White Blood Cell (WBC) counts, packed cell volume (PCV), mean corpuscular volume (MCV) and mean corpuscular haemoglobin (MCH) were not affected by supplementation of yucca powder. However, haemoglobin (HB) concentration was slightly increased and mean corpuscular haemoglobin concentration (MCHC) was significantly increased by 200 ppm yucca powder supplementation to the diet.     

竹酢粉对妊娠后期母羊血液及初乳抗氧化、免疫指标的影响

本试验旨在研究竹酢粉对妊娠后期母羊血液及其初乳抗氧化和免疫指标的影响。试验选择胎次一致、身体状况良好的妊娠后期（妊娠约120 d）湖羊10只,随机分成2组,分别饲喂基础日粮（对照组）和基础日粮+2%竹酢粉（试验组）,试验期3周（1周预饲期+2周正式试验期）,试验结束采集血液测定生化、免疫和抗氧化指标;分娩1周内采集初乳测定免疫和抗氧化指标。结果显示：①试验组母羊血液总蛋白（TP）含量和谷丙转氨酶（GPT）活性显著高于对照组（P<0.05）,谷草转氨酶/谷丙转氨酶（GOT/GPT）和血小板数量则显著低于对照组（P<0.05）,而红细胞数量、血红蛋白浓度均高于对照组母羊,但差异并不显著（P>0.05）;②与对照组相比,试验组母羊血清的免疫球蛋白A（IgA）、白细胞介素-6（IL-6）含量显著升高（P<0.05）,总抗氧化力（T-AOC）、免疫球蛋白G（IgG）和免疫球蛋白M（IgM）含量极显著升高（P<0.01）,肿瘤坏死因子-α（TNF-α）含量低于对照组但无显著差异（P>0.05）;③竹酢粉能够显著降低母羊初乳TNF-α的含量（P<0.05）,显著升高初乳超氧化物歧化酶（SOD）活性（P<0.05）,但对初乳免疫球蛋含量无显著影响（P>0.05）。本研究结果表明,日粮中添加竹酢粉能改善妊娠后期湖羊的健康状况,提高母羊的抗氧化和免疫性能。     

Effects of dietary arginine on hematological parameters and innate immune function of channel catfish

The effects of elevated dietary arginine on the hematology and immune function of juvenile channel catfish Ictalurus punctatus were evaluated by means of in vivo and in vitro experiments. Healthy juvenile channel catfish (average weight, 34.8 g) were fed casein-gelatin-based diets containing 28% crude protein and supplemented with crystalline L-arginine (ARG) at 0.5, 1, 2, or 4% of diet. An intact-protein diet containing 1.3% arginine also was included to investigate the effects of amino acid form (crystalline-free amino acids versus intact protein). Each purified diet was fed to apparent satiation to triplicate groups of fish for 6 weeks. At the end of the experimental feeding period, the fish were injected intraperitoneally with two doses (3 d apart) of 2 mg lipopolysaccharide/kg body weight. Six days after the initial injection, the fish were anesthetized and tissue samples were obtained to evaluate hematological and humoral and cellular immune parameters, including phagocytic activity of peritoneal macrophages, hemoglobin, hematocrit, mean corpuscular volume (MCV), blood cell counts, plasma protein, and hepatic superoxide dismutase activity. High dietary levels (4% ARG) resulted in significantly higher levels of hemoglobin, hematocrit, and circulating erythrocytes. Dietary ARG did not significantly affect MCV and the number of circulating leukocytes, lymphocytes, eosinophils, and monocytes. In vitro, a moderate level (2 mM) of ARG in the culture media was found to be ideal in significantly enhancing phagocytosis. This study demonstrates that some aspects of the immune system of channel catfish are sensitive to changes in dietary ARG.     

浓缩大豆磷脂对肉仔鸡生产性能的影响

本试验采用单因子随机化设计,将225只艾维菌肉仔鸡随机分为5个处理组,每个处理组设3个重复,每个重复15只鸡,以玉米-豆粕型日粮为基础日粮,饲喂处理1设为空白对照组;处理2添加猪油,设为猪油对照组;处理3、4、5分别添加低、中、高水平的浓缩大豆磷脂,为试验组.在0～3周龄猪油以2%,浓缩大豆磷脂分别以2%、3%、4%;在4～6周龄猪油以3%,浓缩大豆磷脂分别以3%、4%、5%添加到基础日粮中以替代相同比例的玉米,蛋白质水平维持不变.研究添加猪油和不同水平的浓缩大豆磷脂对肉仔鸡生长性能的影响,并对其作用机理进行了初步探讨.结果表明:在0～3周龄时,中、高水平试验组极显著地提高了采食量(P<0.01);在4～6周龄时,中水平试验组具有较大的采食量,但高水平试验组采食量有所下降;从全期来看,猪油对采食量影响不大;在0～3周龄,中、高水平试验组极显著地提高了增重(P<0.01),而且在此阶段添加油脂显著提高了饲料转化率(P<0.05);在4～6周龄时,添加油脂极显著提高了增重和饲料转化率(P<0.01);从全期来看,添加油脂可以提高增重与饲料转化率,且在0～3周龄时浓缩大豆磷脂优于猪油.     

妊娠母羊饲喂竹酢粉对羔羊生长性能、血液生理生化指标、免疫与抗氧化能力的影响

试验旨在研究妊娠母羊饲喂竹酢粉对羔羊生长性能、血液生理生化指标、免疫与抗氧化能力的影响。选择初产、妊娠约120 d的健康湖羊50只，随机分为2组，分别饲喂基础日粮（对照组）和基础日粮+2%竹酢粉（试验组），饲喂3周，预饲期1周。分娩后，选择单羔母羊所产、3周龄左右、产期相近（1~4 d）羔羊20只（对照组和试验组各10只）测定其生长性能、血液生理生化、免疫与抗氧化能力等指标。结果表明：试验组羔羊的初生重（BW）、平均日增重（ADG）与对照组相比差异不显著（P>0.05），但日增重提高4.74%。与对照组相比，试验组羔羊血液中白细胞、红细胞、血小板数量和血红蛋白浓度均无显著差异（P>0.05）；血清谷草转氨酶（GOT）活性显著降低（P<0.05），总蛋白（TP）、白蛋白（ALB）、球蛋白（GLB）含量和白蛋白/球蛋白（A/G）有一定程度提高，总胆固醇（TC）含量、丙二醛（MDA）含量、超氧化物歧化酶（SOD）活性和总抗氧化能力（T-AOC）均有降低趋势（P>0.05）；试验组羔羊免疫球蛋白IgA、IgM和IgG含量高于对照组，但均无显著差异（P>0.05）。综上所述，在妊娠母羊日粮中添加2%竹酢粉可在一定程度上提高羔羊日增重，同时羔羊血清总蛋白、白蛋白、球蛋白、IgA、IgM和IgG含量也有一定程度提高。     

Effects of Fusarium mycotoxins with yeast cell wall absorbent on hematology,serum biochemistry,and oxidative stress in broiler chickens

The objectives of the present study were to investigate the toxicity of feed-borne Fusarium mycotoxins on hematology, serum biochemistry, and oxidative stress of broiler chickens, and to evaluate the efficacy of yeast cell wall adsorbent (YCW) in preventing mycotoxins-induced adverse effects. A total of 300 one-day-old Arbor Acres broiler chickens (mixed sex) were randomly divided into 3 groups and received different treatments (5 repetitions per treatment). Treatments were control, naturally contaminated diet (NCD; aflatoxin, 102.08 mg/kg; zearalenone, 281.92 mg/kg; fumonisin, 5,874.38 mg/kg; deoxynivalenol, 2,038.96 mg/kg), and NCD + 2 g/kg of YCW. Broilers fed NCD showed elevated serum alanine transaminase, aspartate transaminase, alkaline phosphatase, γ-glutamyl transferase (GGT), and malondialdehyde (MDA) at 21 d, and alanine transaminase, GGT, creatine kinase, and MDA at 42 d. Higher white blood cell, lymphocyte (LYM), platelet, red blood cell, hemoglobin, hematocrit, and mean corpuscular hemoglobin counts at 21 d, as well as LYM in whole blood and hepatic mRNA expression of heat shock protein 70 at 42 d were also observed in broilers fed with NCD compared with the control diet. However, lower mean corpuscular volume at 42 d and total superoxide dismutase (T-SOD) at both 21 and 42 d were observed in broilers with the NCD treatment when compared with those with the control treatment. Dietary addition of YCW at the level of 2 g/kg in addition to the NCD treatment showed a positive protective effect on GGT, T-SOD in serum, white blood cells, LYM, platelets, and hemoglobin in whole blood at 21 d, and T-SOD, MDA in serum, LYM, mean corpuscular volume in whole blood, and hepatic mRNA expression of heat-shock protein 70 at 42 d. It is suggested that feeding NCD for 42 d might result in a deleterious effect on broiler chickens, and addition of 2 g/kg of YCW can partly attenuate the detrimental effects of the NCD feeding.     

Influence of dietary calcium on lead poisoning in mallard ducks (Anas platyrynchos)

Forty-five mallard ducks were allotted into 3 dietary groups. Group I was fed pelleted calcium-supplemented corn; group II was fed a pelleted commercial duck ration; and group III was fed cracked corn. Ten ducks of each group were given four No. 4 lead shot via an esophageal tube, and 5 ducks of each dietary group were kept as pair-fed controls. Anorexia and weight loss were most severe in the treated group III ducks. Group III had a maximum reduction in food consumption of 87% followed by a slight improvement in appetite; they lost 35% of their initial body weight. Group I treated ducks had a reduction in food consumption of 64% that eventually returned to the quantities consumed at the start of the experiment; group I ducks lost 18% of their initial body weight. Group II treated ducks maintained healthy appetites during the experiment and had a weight gain of 2% of their initial body weight. The number of ducks that became moribund and were euthanatized differed significantly among the treated groups with 100% of group III, 50% of group I, and 0% of group II treated ducks becoming moribund. All 3 groups of treated ducks had increased protoporphyrin IX concentrations compared with controls. Groups I and III lead-treated ducks had significant (P less than 0.05) reductions in erythrocyte counts, PCV, hemoglobin concentrations, and mean corpuscular hemoglobin concentrations as compared with controls. Group II had reduced hemoglobin concentration and mean corpuscular hemoglobin concentration compared with controls.(ABSTRACT TRUNCATED AT 250 WORDS)     

Association of maximum voluntary dietary intake of freeze-dried garlic with Heinz body anemia in horses

OBJECTIVE: To characterize hematologic and clinical consequences of chronic dietary consumption of freeze-dried garlic at maximum voluntary intake in horses. ANIMALS: 4 healthy sex- and age-matched horses. PROCEDURE: An initial garlic dose (0.05 g/kg, twice daily) was fed to 2 horses in a molasses carrier as part of their normal ration and was gradually increased to maximum voluntary intake (0.25 g/kg, twice daily) over 41 days. Dietary supplementation then continued for a total of 71 days. Two control horses were fed molasses with no garlic with their ration. Blood samples were collected weekly and analyzed for hematologic and biochemical changes, including the presence of Heinz bodies. Recovery of affected blood values was followed for 5 weeks after termination of dietary supplementation with garlic. RESULTS: At a daily dose of > 0.2 g/kg, horses fed garlic developed hematologic and biochemical indications of Heinz body anemia, as characterized by increases in Heinz body score (HBS), mean corpuscular volume (MCV), mean corpuscular hemoglobin, platelet count, and serum unconjugated and total bilirubin concentrations and decreases in RBC count, blood hemoglobin concentration, mean corpuscular hemoglobin concentration, and serum haptoglobin concentration. Recovery from anemia was largely complete within 5 weeks after termination of dietary supplementation with garlic. Heinz body score and MCV remained high at the end of the 5-week recovery period. CONCLUSIONS AND CLINICAL RELEVANCE: Horses will voluntarily consume sufficient quantities of garlic to cause Heinz body anemia. The potential for garlic toxicosis exists when horses are chronically fed garlic. Further study is required to determine the safe dietary dose of garlic in horses.     

饲粮添加不同水平苜蓿粗多糖对蛋鸡生产性能和蛋品质的影响

本试验旨在研究饲粮添加不同水平苜蓿粗多糖对蛋鸡生产性能和蛋品质的影响。选取540只27周龄的海兰褐壳蛋鸡,随机分为6组,每组6个重复,每个重复15只鸡。对照组饲喂基础饲粮,试验组分别饲喂在基础饲粮中添加250、500、1 000、2 000和4 000 mg/kg苜蓿粗多糖的试验饲粮。试验期24周。结果表明:与对照组相比,1)试验1~8周,500、2 000和4 000 mg/kg苜蓿粗多糖添加组产蛋率均显著提高(P0.05);试验9~16周、17~24周和1~24周,250、500、1 000和2 000 mg/kg苜蓿粗多糖添加组产蛋率显著提高(P0.05),料蛋比显著降低(P0.05),且在试验17~24周和1~24周,总产蛋重量显著提高(P0.05),而平均蛋重和采食量无显著差异(P0.05);2)250、500和1 000 mg/kg苜蓿粗多糖添加组在试验第6、8、12、16、20和24周蛋壳颜色显著加深(P0.05),且在试验第8、12、16周较4 000 mg/kg苜蓿粗多糖添加组蛋壳颜色亦显著加深(P0.05);3)2 000 mg/kg苜蓿粗多糖添加组在试验第6、20、24周蛋壳强度显著提高(P0.05);4)试验第8、12、16、20和24周,250、500、1 000和2 000mg/kg苜蓿粗多糖添加组蛋壳厚度显著增加(P0.05),且4 000 mg/kg苜蓿粗多糖添加组在试验第8、20、24周蛋壳厚度亦显著增加(P0.05);5)2 000 mg/kg苜蓿粗多糖添加组在试验第10、12和24周蛋白高度和哈夫单位显著提高(P0.05),且2 000 mg/kg苜蓿粗多糖添加组在试验第10和12周蛋白高度和哈夫单位显著高于250、1 000和4 000 mg/kg苜蓿粗多糖添加组(P0.05)。试验表明,产蛋期蛋鸡饲粮中添加适宜水平的苜蓿粗多糖可以显著提高蛋鸡生产性能,改善蛋品质,且推荐适宜添加水平为250 mg/kg。     

灵芝发酵产物对感染PCV-2病毒断奶仔猪生长性能和免疫功能的影响

本研究以深层发酵灵芝为原料,评估灵芝发酵产物对感染猪圆环病毒-2(PCV-2)断奶仔猪生长发育及免疫功能的影响.试验1:选择160头21 d断奶的仔猪,随机分为4组,每组4个重复,每个重复10头猪.各组分别在基础日粮中添加0、50、100和150 mg/kg灵芝发酵产物.试验2:将120头21 d断奶的仔猪随机分为2组...     

Hematology and Clinical Chemistry in Amiata Donkey Foals from Birth to 2 Months of Age

The aim of the present study was to fill the void in data related to hematological and biochemical parameters of donkey foals. Whole-blood and plasma samples were obtained from 16 Amiata donkey foals at birth, at 24 and 48 hours, and at 1, 2, 3, 4, 6, and 8 weeks of age. RBC, WBC, hemoglobin concentration (Hgb), mean corpuscular volume, mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration, platelet count, glucose, creatinine, blood urea, triglycerides, total cholesterol, total bilirubin, aspartate aminotransferase, γ-glutamyl transferase, creatine-phosphokinase, alkaline phosphatase, total calcium, potassium, sodium, phosphorus, and albumin were measured. Similar to equine foals, values of RBC, Hgb, and Hct decreased significantly after the first 48 hours of life in Amiata donkey foals, reaching values similar to adult donkeys. No changes were found for mean corpuscular volume and mean corpuscular hemoglobin concentration during the study period. The WBC was low at birth when compared with subsequent measurements, but significantly increased in the subsequent surveys. Platelet count was lower in the first week, with a secondary peak 2 weeks later, and then a decline again up to the eighth week. In agreement with equine foals, electrolyte concentrations, triglycerides, and total cholesterol did not show significant differences. Creatinine, total bilirubin, and blood urea showed a trend similar to RBC, Hgb, and Hct. For the first time, data of hematological and biochemical parameters in Amiata donkey foals are provided.     

碱性矿物质对比目鱼生长性能、养分消化率和非特异性免疫的影响

文章旨在评估一种商品复合碱性矿物质对比目鱼生长性能、养分消化利用及非特异性免疫的影响.试验选择平均初始体重为(144.06±0.17)g的比目鱼900条,随机分为3组,每组5个水族箱,每个水族箱饲养60条.对照组饲喂基础日粮,处理组日粮分别在基础日粮中添加2和4?mg/kg碱性矿物质,试验持续105?d.结果:随着日粮...     

酵母铬对不同应激状态蛋鸡产蛋性能及血清生化指标的影响

选20周龄感染鸡传染性鼻炎、25周龄康复的罗曼产蛋鸡450只 ,随机等分5组 ,在相同基础日粮中添加0(对照)、0.2、0.4、0.6及0.8mg/kg铬量的酵母铬 ,经2周高温期预试、5周高温期及3周舒适期正试。结果表明 :(1)与舒适期相比 ,酵母铬显著提高热应激期产蛋率(P<0.01);补铬有降低试验期蛋重的趋势(P>0.05)。(2)热应激期高浓度酵母铬显著提高血清T4、胰岛素和肌酸激酶水平(P<0.01或P<0.05) ;降低了血糖水平(P<0.05)。低浓度酵母铬可提高试验期蛋鸡血清碱性磷酸酶含量(P<0.05) ,血清蛋白和胆固醇则受鸡的应激和生理状态及铬水平的影响