宫炎清灌注液治疗奶牛子宫内膜炎临床试验

考察宫炎清灌注液治疗奶牛子宫内膜炎临床疗效。方法：选取临床上已发生子宫内膜炎的奶牛．将宫炎清灌注液加温至37℃左右，进行子宫灌注，100ml／次，隔日一次，四次为一个疗程：结果：宫炎清灌注液对奶牛子宫内膜炎的治疗其有效率为100％．治愈率为85．7％。     

纳米银对奶牛子宫内膜炎的临床治疗试验

以不同药物对子宫内膜炎患牛分别进行临床治疗。以比较用化学还原法制取的纳米银与盐酸土霉素、环丙沙星、甲砜霉素药物对奶牛子宫内膜炎治疗的疗效。结果表明，纳米银对奶牛子宫内膜炎的治愈率、有效率分别是91．67％和100％，与其他组相比差异显著。说明纳米银对奶牛子宫内膜炎具有较好的疗效。     

盐酸土霉素及清宫液治疗奶牛子宫内膜炎试验

对７７头患子宫内膜炎的奶牛进行治疗试验，其中４６头患牛采用盐酸土霉素治疗，３１头患牛采用清宫液治疗，治疗结果：土霉素治疗组治愈率８０．４３％，好转率１０．８６％，无效８．６％；清宫液治疗组治愈率８７．０９％，好转率６．４５％无效６．４５％。试验结果表明：盐酸土霉素及清宫液治疗奶牛子宫内膜炎的效果显著，可在生产实践中推广应用。     

乙烯雌酚加土霉素配合治疗奶牛子宫内膜炎

选择顽固性、卡他性及慢性子宫内膜炎奶牛65例,运用乙烯雌酚和土霉素配合进行临床治疗试验。结果表明,临床上配合运用乙烯雌酚和土霉素对奶牛子宫内膜炎疗效显著,对照组奶牛用药后经15~20天治愈,治愈率为65%;试验组奶牛用药后经7~10天治愈,治愈率为95%。表明乙烯雌酚与土霉素配合使用具有抑菌消炎、诱导发情和恢复子宫机能的作用。     

复方中草药灌注液治疗奶牛子宫内膜炎临床效果观察

目的 研究旨在通过试验观察中草药治疗奶牛子宫内膜炎疗效,以其为临床应用中草药治疗奶牛子宫内膜炎提供参考。方法 将60 头患子宫内膜炎奶牛,随机分成5 组,每组12 头,复方中草药高、中、低剂量组（分别子宫灌注150 mL/头、100 mL/头和50 mL/头）、土霉素组（子宫灌注50 mL/头）和对照组。结果 复方中草药中剂量组和高剂量组的总有效率为75.00%和83.33%,均高于土霉素组的总有效率66.67%;土霉素组在治疗结束后的平均发情天数缩短了14 d,所有复方中草药组的发情天数也缩短了11~19 d;3 个情期内土霉素组受胎率为50.00%,复方中草药组受胎率介于41.67%~66.67%之间。结论 复方中草药对奶牛子宫内膜炎的治疗效果优于土霉素,其中以高剂量组效果最佳。     

科苑采撷

用溶菌酶治疗奶牛子宫内膜炎效果好经临床１３６例治疗奶牛产后子宫内膜炎试验观察，溶菌酶对奶牛子宫内膜炎的总治愈率、治疗后各组子宫恢复的程度均较抗生素治疗组和空白对照组明显好，对子宫内膜炎的治愈率为８２．３５％～８８．２４％，明显好于金霉素的治愈率５６．２５％。溶菌酶试验组平均受胎率为７５．３８％，明显高于抗生素对照组的５６．２５％，空白对照组的１５．６３％。治愈牛产后至初配平均天数也较短。纤维素酶在蛋鸡料中的作用在蛋鸡常规饲料中添加０．１％复合纤维素酶，试验组的产蛋率比对照组提高１０．８％，蛋重提…     

溶葡萄球菌酶对奶牛子宫内膜炎的临床治疗试验

溶葡萄球菌酶是从Staphylococcus Simulans培养物中提取的一种肽链内切酶,在较低浓度下能迅速裂解葡萄球菌细胞壁而呈现较强的杀菌活性且不易产生耐药性。为探讨溶葡萄球菌酶在防治奶牛子宫内膜炎上的应用,本试验选择140头患子宫内膜炎的奶牛,通过子宫内灌注给药的方式,每次分别灌注400u、800u和1600u溶葡萄球菌酶,连续给药3次,并以1O％Y-霉素注射液为对照,进行临床治疗试验。结果显示,溶葡萄球菌酶高、中、低剂量的治愈率分别为90．0％、73．3％和36．7％,对照药物治愈率为70．0％;在繁殖性能方面,高、中剂量组的奶牛情期内受胎率和总受胎率等明显高于低剂量组。表明溶葡萄球菌酶是一种治疗奶牛子宫内膜炎效果良好的生物类药物。     

聚维酮碘制剂治疗奶牛子宫内膜炎疗效观察

为了解聚维酮碘治疗奶牛子宫内膜炎的临床效果,选择患有不同类型子宫内膜炎的奶牛80例,采用4%聚维酮碘进行临床治疗试验,土霉素做对照。结果表明:4%聚维酮碘组与土霉素对照组的有效率和受孕率分别为84.4%和73.1%,82.9%和68.6%,差异均不显著(P>0.05)。     

不同药物治疗奶牛子宫内膜炎的临床观察

奶牛子宫内膜炎是奶牛最常见的多发病之一，奶牛的繁育和奶牛场的经济效益造成了极大的影响，对奶牛子宫内膜炎的治疗具有十分重要的意义。先后选择了患有子宫内膜炎的奶牛40头，随机分为5组，每组8头，分别采用临床上较常用的5种方剂进行对比治疗，结果方剂乳酸环丙沙星配合缩宫素的治疗效果最为良好，一个疗程（3d）后，其治愈率达到了85％，而且作用迅速。所以乳酸环丙沙星配合缩宫素对治疗奶牛子宫内膜具有良好的临床应用和推广价值。     

重组溶葡萄球菌酶对奶牛子宫内膜炎的疗效研究

为研究重组溶葡萄球菌酶对奶牛子宫内膜炎的疗效,以青霉素钠、土霉素为对照药物,子宫内灌注给药,分别在河北、内蒙古、广州、上海奶牛场试验,共治疗急、慢性子宫内膜炎病例359头,抽检249头奶牛子宫分泌物进行细菌学分离鉴定与统计分析。结果表明,大肠杆菌、金黄色葡萄球菌、链球菌、化脓棒状杆菌是4种检出率较高的病原菌。重组溶葡萄球菌酶治疗组与土霉素、青霉素钠比较,按400、800、1200U／次的三个浓度剂量治疗,隔日投药1次,连续用药3次,800、1200U／次的浓度剂量显效率超过80％,显著优于青霉素钠的疗效（P〈0．01）,与土霉素注射液组相当。追加用药次数可进一步提高奶牛子宫内膜炎的痊愈率。重组溶葡萄球菌酶能有效治疗由金黄色葡菌球菌、链球菌、化脓棒状杆菌等革兰氏阳性菌感染的子宫内膜炎,治愈后受孕率在70％左右。尤其是对抗生素治疗无效的慢性子宫内膜炎同样具有很好的疗效。因此,用重组溶葡萄球菌酶治疗奶牛子宫内膜炎安全、高效。     

应用激素配合抗生素治疗奶牛子宫内膜炎的效果

应用氯前列稀醇和两厢情注射液配合抗生素治疗奶牛子宫内膜炎患牛35头,其治疗效果分别达94.7%和93.8%,单纯应用抗生素治疗子宫内膜炎5头,其治愈率为80%。且两个情期受胎率实验组明显高于对照组。     

复方蒲公英喷雾剂对奶牛隐性乳房炎的疗效观察

运用复方蒲公英喷雾剂对奶牛隐性乳房炎进行疗效观察。将CMT法检测呈阳性的奶牛分3组：复方蒲公英喷雾剂组,中药对照组,空白对照组。给药后用CMT诊断液检测治疗情况。结果发现,复方蒲公英喷雾剂对奶牛隐性乳房炎的治愈率达88.9%,有效率97.2%;中药对照组治愈率56.5%,有效率82.6%。表明复方蒲公英喷雾剂对奶牛隐性乳房炎有良好的治疗效果,使用方便,适于临床应用。     

生物氧疗法在治疗奶牛临床型乳房炎上的应用

本试验采用生物氧(成分为臭氧)结合抗生素治疗奶牛临床型乳房炎,并对生物氧疗法的疗效进行评价。2009年9月份~11月份,选取患有临床型乳房炎的奶牛30头并随机分为两组,每组15头。试验组采用生物氧结合抗生素疗法,对照组只采用抗生素疗法。结果发现,试验组平均治愈时间为7.4d,治愈14头,治愈率为93.33%,而对照组平均治愈时间为12.6d,治愈11头,治愈率为73.33%。从试验结果可以发现生物氧疗法在治疗奶牛临床型乳房炎上效果显著,有良好的应用前景。     

奶牛子宫内膜炎非抗菌治疗试验

选用８６头患子宫内膜炎的奶牛分为３组进行非抗菌治疗试验，１，２，３组分别采用催产素，氯前列烯醇和碘溶液治疗。结果表明：１，２，３组的治愈率，好转率和无效率分别为８０，５％，１２．２％，７．３％，８６．４％，９．１％，４．１％，６９．６０％，２１．７％，８．７％。     

Study on Efficiency of Balloon Technique against Infertility in Dairy Cows with Metritis

Uterine disease after parturition is present in up to 40% of dairy cows and can cause cow infertility,which restricts efficiency of the cow production and leads to large economic loss.System administration and intrauterine infusion are widely utilized to treat cow endometritis.In recent years,the balloon technique is used gradually.Uterus washing pipe is used to inject liquid into the uterus and then the liquid and the inflammatory substances are discharged by the veterinarian.After the inflammatory substances cleaning up,the drug is delivered into the uterus.In order to provide a new method that can treat the infertility in dairy cows with metritis,323 cows which were diagnosed with metritis were used to detect the efficiency of balloon technique,the location of the balloon and the different drugs perfusion against infertility in dairy cows with metritis.The results showed that the balloon technique was showed remarkable effect against cow metritis comparing to the conventional technique.The pregnancy rate of the balloon technique was more than 60%,by contrast,the conventional technique was about 35%.Compared with being placed in the cornua uteri,the efficacy was more obvious when the balloon was placed in the uterine body,and the pregnancy rate was more than 60%.However,the pregnancy rate of another method was almost 40%.There were prominent curative effect of the three drug combinations.The pregnancy rates of the saline+Oxytetracycline,Lutajing+Gongdekang and saline were more than 70%,60% and 55%, respectively.Therefore,compared to the conventional technique and cornua uteri technique,balloon technique（uterine body）significantly increases the pregnancy rate,which is beneficial to be widely used in production.So it is significant that the new technique used widely is important for improving the production efficiency of dairy cows.     

气囊子宫体冲洗新方法治疗奶牛子宫炎引起不孕症的效果研究

分娩后奶牛发生子宫炎症的概率高达40%,子宫炎引发的不孕症是制约奶牛业生产效率的主要因素之一,造成了巨大经济损失。对于子宫炎的治疗主要有传统的子宫内灌注给药和系统给药方法。近年来,气囊子宫冲洗法逐渐被应用,其利用专用子宫冲洗管,将气囊置于奶牛子宫,注入液体冲洗子宫后,人工把液体及炎性物质全部排出,冲洗干净后投药。本研究利用323头被诊断为子宫炎的奶牛,进行气囊法与常规法治疗效果、气囊所放位置和冲洗用药对治疗效果的影响研究。结果表明,与常规法相比,气囊子宫体冲洗法提高了子宫炎奶牛的配种率,气囊法配种率达60%以上,而常规法配种率仅为35%左右。气囊子宫体冲洗法治疗效果明显优于子宫角放置法,气囊子宫体冲洗法配种率达60%以上,而气囊子宫角冲洗法治疗后其配种率仅为40%左右,且子宫体冲洗法节省时间、易于操作,安全系数高。3种药物组合都取得明显效果,生理盐水+土霉素子宫注射剂法的配种率达70%以上,露它净+宫得康法的配种率达60%以上,只用生理盐水冲洗的配种率也达55%以上。因此,与子宫内灌注给药、气囊子宫角放置冲洗等方法相比,气囊子宫体冲洗法显著提高了子宫炎奶牛的配种率,适用于在规模化生产中进行应用。这一新方法的推广对提高奶牛生产效率具有重要意义。     

Risk factors for postpartum endometritis and the effect of endometritis on reproductive performance in dairy cows in Korea

The objectives of this study were to determine the risk factors for postpartum endometritis by evaluating several reproductive factors in individual cows, and to determine the effect of endometritis on the subsequent reproductive performance in dairy herds in Korea. The data, including health status, parity and body condition score (BCS) of cows, and calving date, were collected from 320 cows in eight dairy herds calving from January 2001 to October 2002. We used logistic regression to evaluate the effects of these factors on endometritis. A stepwise procedure, used to obtain the appropriate model with alpha=0.05, revealed that retained placenta, metabolic disorders and parity are the important risk factors for endometritis. The mean intervals from calving to first service and conception were prolonged (P<0.01) by 23 and 36 days, respectively, in the endometritis group compared to the non-endometritis group. The first service conception rate was lower (P<0.05) in the endometritis group (47.6%) than in the non-endometritis group (62.6%). The number of services per conception was higher (P<0.05) in the endometritis group (1.9) than in the non-endometritis group (1.6). We conclude that retained placenta, metabolic disorders and cow parity are strongly correlated with the development of postpartum endometritis, which decreases reproductive performance in dairy herds in Korea.     

中药复方灌注剂对奶牛乳房炎的防治效果及免疫增强作用

选取临床型、隐性型和健康型荷斯坦奶牛共80头,用于中药复方灌注剂对奶牛乳房炎的防治及免疫试验,对治疗前后乳中体细胞数(SCC)、乳清中白蛋白(SA)、乳酸脱氢酶(LDH)、丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、淋巴细胞刺激指数(SI)、嗜中性粒细胞吞噬率和使用前后干奶期乳房炎的发病率进行了研究。结果表明,中药复方灌注剂和抗生素对隐性乳房炎的治愈率分别为94.44%和88.24%,有效率均为100%;对临床型乳房炎的治愈率分别为86.67%和76.92%,有效率为93.33%和84.62%;对干奶期乳房炎的预防效果分别为88.89%和94.12%,而生理盐水对照组发病率高达45.45%。中药复方灌注剂可以极显著降低患病奶牛乳中SCC、SA、LDH、ALT、AST水平(P＜0.01),提高SI和嗜中性粒细胞吞噬率(P＜0.01)。试验证实了中药复方灌注剂对奶牛乳房炎有较好的防治效果,能够修复奶牛受损的乳腺上皮细胞,降低受损细胞产生的酶含量和血乳的渗透性,提高患病奶牛机体的免疫机能。     

中草药防治奶牛不孕症研究

奶牛不孕症严重影响乳业生产的发展。根据发展乳业生产的需要和祖国医学的特点,我们应用中草药补中益气汤加减,促进产后母牛子宫复旧。试验组80头牛,对照组71头牛,试验组母牛发情时间较对照组提早20.17天;试验组母牛在产后100天内的受胎率为60％(45/75);对照组100天内的受胎率为40.6％(28/69),两者差异显著(P<0.05)。应用复方仙阳汤治疗母牛产后卵巢静止和持久黄体性不孕症150例,发情排卵140头,有效率为93.3％;配种受胎120头,受胎率为85.7％。     

Ulcerative blepharitis and conjunctivitis in adult dairy cows and association with Moraxella bovoculi

Nine lactating dairy cows were evaluated because of eye lesions. Examination revealed mild to severe ulceration of the lower and/or upper eyelids, mild to severe swelling surrounding affected eyes, and profuse lacrimation. Lesions typically affected 1 eye, and involved the eyelid skin and conjunctiva. Oxytetracycline treatment led to cure within 2 wk.