Response of giant reed (Arundo donax L.) to nitrogen fertilization and soil water availability in semi-arid Mediterranean environment

The aim of the present work was to evaluate the effect of soil water availability and nitrogen fertilization on yield, water use efficiency and agronomic nitrogen use efficiency of giant reed (Arundo donax L.) over four-year field experiment.After the year of establishment, three levels for each factor were studied in the following three years: I₀ (irrigation only during the year of establishment), I₁ (50% ETm restitution) and I₂ (100% ETm restitution); N₀ (0 kg N ha⁻¹), N₁ (60 kg N ha⁻¹) and N₂ (120 kg N ha⁻¹).Irrigation and nitrogen effects resulted significant for stem height and leaf area index (LAI) before senescence, while no differences were observed for stem density and LAI at harvest.Aboveground biomass dry matter (DM) yield increased following the year of establishment in all irrigation and N fertilization treatments. It was always the highest in I₂N₂ (18.3, 28.8 and 28.9 t DM ha⁻¹ at second, third and fourth year growing season, respectively). The lowest values were observed in I₀N₀ (11.0, 13.4 and 12.9 t DM ha⁻¹, respectively).Water use efficiency (WUE) was significantly higher in the most stressed irrigation treatment (I₀), decreasing in the intermediate (I₁) and further in the highest irrigation treatment (I₂). N fertilization lead to greater values of WUE in all irrigation treatment.The effect of N fertilization on agronomic nitrogen use efficiency (NUE) was significant only at the first and second growing season.Giant reed was able to uptake water at 160–180 cm soil depth when irrigation was applied, while up to 140–160 cm under water stress condition.Giant reed appeared to be particularly suited to semi-arid Mediterranean environments, showing high yields even in absence of agro-input supply.     

Influence of harvest time and frequency on light interception and biomass yield of festulolium and tall fescue cultivated on a peatland

In this study, we report efficiencies of light capture and biomass yield of festulolium and tall fescue cultivated on a riparian fen in Denmark under different harvesting managements. Green biomass targeted for biogas production was harvested either as two cuts (2C) or three cuts (3C) in a year. Three different timings of the first cut in the 2C systems were included as early (2C-early), middle (2C-mid) and late (2C-late) cuts corresponding to pre-heading, inflorescence emergence and flowering stages, respectively. The fraction of intercepted photosynthetically active radiation (f_PAR) was derived from the canopy reflectance measured on 61 dates throughout a year, and cumulative interception of PAR (IPAR) and radiation use efficiency (RUE) was calculated. The dynamics of f_PAR and biomass accumulations was similar for both crops before the first cuts in all managements. Festulolium f_PAR in 2C-early and 2C-mid managements declined faster than in 2C-late and 3C managements in the second growth period and thus growing period IPAR of 2C-early and 2C-mid declined by 8% as compared to 3C management where IPAR was 925 MJ m⁻². Annual festulolium dry matter (DM) yield in 2C-early and 2C-mid managements (average 14.1 Mg DM ha⁻¹) decreased by 22% compared to 3C management (18.2 Mg DM ha⁻¹). The highest and the lowest RUE of festulolium were observed in 3C and 2C-mid managements as 1.97 and 1.59 g MJ⁻¹, respectively. For tall fescue f_PAR declined rather slowly in the second growing period in all 2C managements, which contributed to similar IPAR (908–919 MJ m⁻²), total biomass yield (16.4–18.8 Mg DM ha⁻¹ yr⁻¹) and RUE (1.80–2.07 g MJ⁻¹) for all managements. Whereas both crops were highly productive under both 3C management and 2C management with first harvest after flowering (i.e., 2C-late), the 2C-late strategy is recommended as the least intensive of the two management systems.     

Radiation use efficiency,chemical composition,and methane yield of biogas crops under rainfed and irrigated conditions

For biomethane production, the cup plant (Silphium perfoliatum L.) is considered a promising alternative substrate to silage maize (Zea mays L.) due to its high biomass potential and associated ecological and environmental benefits. It has also been suggested to grow cup plant on less productive soils because of its presumed drought tolerance, but robust information on the impact of water shortage on biomass growth and substrate quality of cup plant is rare. Therefore, this study assesses the effects of soil water availability on the chemical composition and specific methane yield (SMY) of cup plant. Furthermore above-ground dry matter yield (DMY) was analysed as a function of intercepted photosynthetic active radiation (PAR) and radiation use efficiency (RUE). Data were collected in a two-year field experiment under rainfed and irrigated conditions with cup plant, maize, and lucerne-grass (Medicago sativa L., Festuca pratensis Huds., Phleum pratense L.). The cup plant revealed a slight decrease of −6% in the SMY in response to water shortage (less than 50% of plant available water capacity). The average SMY of cup plant [306 l (kg volatile solids (VS))⁻¹] was lower than that of maize [362 l (kg VS)⁻¹] and lucerne-grass [334 l (kg VS)⁻¹]. The mean drought-related reduction of the methane hectare yield (MHY) was significantly greater for cup plant (−40%) than for maize (−17%) and lucerne-grass (−13%). The DMY reduction in rainfed cup plant was mainly attributed to a more severe decrease in RUE (−29%) than for maize (−16%) and lucerne-grass (−12%). Under water stress, the mean cup plant RUE (1.3 g MJ⁻¹) was significantly lower than that of maize (2.9 g MJ⁻¹) and lucerne-grass (1.4 g MJ⁻¹). Compared to RUE, the reduced PAR interception was less meaningful for DMY in rainfed crops. Hence, the cup plant is not suitable for growing on drought prone lands due to its high water demand required to produce reasonably high MHYs.     

Effect of irrigation and nitrogen fertilization on the production of biogas from maize and sorghum in a water limited environment

The expansion of biogas production from anaerobic digestion in the Po Valley (Northern Italy) has stimulated the cultivation of dedicated biomass crops, and maize in particular. A mid-term experiment was carried out from 2006 to 2010 on a silt loamy soil in Northern Italy to compare water use and energy efficiency of maize and sorghum cultivation under rain fed and well-watered treatments and at two rates of nitrogen fertilization. The present work hypothesis were: (i) biomass sorghum, for its efficient use of water and nitrogen, could be a valuable alternative to maize for biogas production; (ii) reduction of irrigation level and (iii) application of low nitrogen fertilizer rate increase the efficiency of bioenergy production. Water treatments, a rain fed control (I0) and two irrigation levels (I1 and I2; only one in 2006 and 2009), were compared in a split–split plot design with four replicates. Two fertilizer rates were also tested: low (N1, 60 kg ha⁻¹ of nitrogen; 0 kg ha⁻¹ of nitrogen in 2010) and high (N2, 120 kg ha⁻¹ of nitrogen; 100 kg ha⁻¹ of nitrogen in 2010). Across treatments, sorghum produced more aboveground biomass than maize, respectively 21.6 Mg ha⁻¹ and 16.8 Mg ha⁻¹ (p < 0.01). In both species, biomass yield was lower in I0 than in I1 and I2 (p < 0.01), while I1 and I2 did differ significantly. Nitrogen level never affected biomass yield. Water use efficiency was generally higher in sorghum (52 kg ha⁻¹ mm⁻¹) than in maize (38 kg ha⁻¹ mm⁻¹); the significant interaction between crop and irrigation revealed that water use efficiency did not differ across water levels in sorghum, whereas it significantly increased from I0 and I1 to I2 in maize (p < 0.01). The potential methane production was similar in maize and sorghum, while it was significantly lower in I0 (16505 MJ ha⁻¹) than in I1 and I2 (21700 MJ ha⁻¹). The only significant effect of nitrogen fertilization was found in the calculation of energy efficiency (ratio of energy output and input) that was higher in N1 than in N2 (p < 0.01). These results support the hypothesis that (i) sorghum should be cultivated rather than maize to increase energy efficiency, (ii) irrigation level should replace up to 36% of ETr and (iii) nitrogen fertilizer rate should be minimized to maximize the efficiency in biomass production for anaerobic digestion in the Po Valley.     

Optimized single irrigation can achieve high corn yield and water use efficiency in the Corn Belt of Northeast China

Decreasing the corn (Zea mays L.) gap between the potential yield and farm yield and reducing the risk of grain yield of drought are very important for corn production in the Corn Belt of Northeast China (CBNC). To achieve a high and stable corn yield, the effects of supplementary irrigation on yield, water use efficiency (WUE) and irrigation water use efficiency (IWUE) were studied using a modelling approach. The Root Zone Water Quality Model 2 was parameterized and evaluated using two years of experimental data in aeolian sandy soil and black soil. The evaluated model was then used to investigate responses to various irrigation strategies (rainfed, full irrigation and 12 single irrigation scenarios) using long-term weather data from 1980 to 2012. Full irrigation guarantees a high and stable corn grain yield (12.92 Mg ha⁻¹ and has a coefficient of variation (CV) of 14.8% in aeolian sandy soil; 12.30 kg Ma⁻¹ and CV of 11.1% in black soil), but has a low water use efficiency (19.92 and 21.81 kg ha⁻¹ mm⁻¹) and a low irrigation water use efficiency (10.01 and 11.03 kg ha⁻¹ mm⁻¹). A single irrigation can increase corn yields by 3–35% for aeolian sandy soil and 5–35% for black soil over different irrigation dates compared with no irrigation. The most suitable single irrigation date was during late June to early July for aeolian sandy soil (yield = 10.73 Mg ha⁻¹ and WUE = 27.94 kg ha⁻¹ mm⁻¹) and early to mid-July for black soil (yield = 11.20 Mg ha⁻¹ and WUE = 27.70 kg ha⁻¹ mm⁻¹). The lowest yield risk of falling short of the yield goal of 8, 9, and 10 Mg ha⁻¹ were 9.1%, 18.2%, and 33.33% in aeolian sandy soil and 3.0%, 15.25, and 21.2% in black soil when an optimized single irrigation was applied in late June or early July, respectively. Therefore, an optimized single irrigation should be applied in late June to early July with the irrigation amount to refill soil water storage of root zone to field capacity in CBNC.     

Old tall durum wheat cultivars are suited for dual-purpose utilization

The lateness, tallness and high vigour of old tall durum wheat cultivars could be advantageous for dual-purpose use and their high propensity for lodging should be reduced by grazing. A 3-year field trial was performed in Sardinia, Italy, in a typical Mediterranean environment. Crops of the durum wheat cultivar Senatore Cappelli were sown in October, and grazing was simulated by clipping half of the plots at the terminal spikelet stage of development. The forage biomass derived from clipping varied greatly between seasons (from 0.8 to 3.3 t ha⁻¹ dry matter) in response to the notable inter-seasonal variability in weather conditions. Cultivar Senatore Cappelli showed good recovery following clipping, with the ability to attain almost complete radiation interception well before anthesis. The high number of leaves that emerged after clipping might have contributed to this good recovery. Nevertheless, clipping reduced the dry matter produced by anthesis (16 t ha⁻¹ in clipped compared to 21 t ha⁻¹ in unclipped crops) as well as the final dry matter (DM_MAT) (19 t ha⁻¹ in clipped compared to 23 t ha⁻¹ in unclipped crops), although these differences disappeared when the clipped biomass was included. The lower lodging observed at anthesis in the clipped (21%) compared with unclipped crops (63%) likely reduced the difference between treatments. The lower DM_MAT of clipped treatments was reflected in a lower grain yield (GY) (3.4 t ha⁻¹ vs 4.2 t ha⁻¹ in the unclipped treatment). Clipping did not affect the amount of nitrogen present in the biomass, nitrogen uptake efficiency or radiation use efficiency. GY reduction after clipping was mediated by the reduction in spikes m⁻² and kernels m⁻² (KNO). Spike fertility was not affected by clipping, because the same amount of radiation was available for each spike (about 1 MJ). The period with reduced ground cover after clipping was reflected in an increased evaporation and reduced transpiration, which did not alter the total water used and increased the transpiration efficiency in terms of DM_MAT.Old tall durum wheat cultivars manifested good suitability for dual-purpose use in environments with low attainable yields because their low grain yield potential contributed to reducing the negative effects of clipping on GY. Their high straw yield and kernel protein percentage represented an advantage with respect to semi-dwarf cultivars.     

Effects of cinnamon extract,chitosan coating,hot water treatment and their combinations on crown rot disease and quality of banana fruit

The antifungal activities of cinnamon extract (CE), piper extract (PE) and garlic extract (GE) were evaluated on banana crown rot fungi (Colletotrichum musae, Fusarium spp. and Lasiodiplodia theobromae) in vitro. The assay was conducted with extracts of CE, PE and GE with concentrations of 0, 0.1, 0.5, 1.0, 5.0, 10.0 and 0.75 g L⁻¹ of carbendazim (CBZ) on potato dextrose agar at room temperature. CE completely inhibited conidial germination and mycelial growth of all fungi at 5.0 g L⁻¹. PE totally suppressed mycelial growth of all fungi at 5.0 g L⁻¹ and conidial germination at 10.0 g L⁻¹ except for Fusarium spp. GE had no significant effects but low concentrations (0.1 and 0.5 g L⁻¹) enhanced germ tube elongation of the three fungi. The ED₅₀ values were higher for mycelial growth than for conidia except for Fusarium spp. Combined treatments were investigated on crown rot development in banana fruit (Musa AAA group ‘Kluai Hom thong’). Treatments included 5.0 g L⁻¹ CE, 1% (w/v) chitosan solution, hot water treatment (HWT, 45 °C for 20 min), CE plus chitosan, CE plus HWT and 0.75 g L⁻¹ of CBZ, applied before and after inoculation of the fruit. Crown rot development was assessed during storage at 13 °C for 7 weeks. Disease development was least (25%) on CE treated fruit after inoculation compared to CBZ but was higher when CE was applied before inoculation. Chitosan significantly delayed ripening as in terms of peel color, firmness, soluble solids and disease severity. CE showed no negative effects on quality of fruit. CE plus HWT caused unacceptable peel browning.     

Development of critical nitrogen dilution curve in rice based on leaf dry matter

The critical nitrogen (N_c), defined as the minimum N concentration required for maximum growth, is proposed for diagnosis of the in-season N status in crop plants. It has been established for several crops including rice on whole-plant dry matter (DM) basis but has not been determined for canopy leaf basis. This research was undertaken to develop a new N_c dilution curve based on leaf dry matter (L_DM) and to assess its applicability to estimate the level of N nutrition for Japonica rice in east China. Three field experiments were conducted with varied N rates (0–360 kg N ha⁻¹) and three Japonica rice (Oryza sativa L.) hybrids, Lingxiangyou-18 (LXY-18), Wuxiangjing-14 (WXJ-14) and Wuyunjing (WYJ) in Jiangsu province of east China. Five hills from each plot were sampled from active tillering to heading for growth analysis and leaf N determination. The N_c dilution curve on leaf N concentration was described by the equation N_c = 3.76W^−0.218, when L_DM ranged from 0.67 to 4.25 t ha⁻¹. However, for L_DM < 0.67 t ha⁻¹, the constant critical value N_c = 4.09%L_DM was applied. This N_c dilution curve on L_DM basis was slightly higher than the curves on plant DM basis in Japonica rice, yet both lower than the reference curve of high yielding Indica rice in tropics. The N nutrition index (NNI) and accumulated N deficit (N_and) of leaves ranged from 0.65 to 1.06 and 79.62 to −6.39 kg ha⁻¹, respectively, during main growth stages under varied N rates in 2010 and 2011. The results indicate that the present N_c dilution curve and derived NNI and N_and adequately identified the situations of N-limiting and non-N-limiting nutrition in two rice varieties and could be used as reliable indicators of N status during growth of Japonica rice in east China.     

Short and long-term effects of different irrigation and tillage systems on soil properties and rice productivity under Mediterranean conditions

In Mediterranean environments, flood irrigation of rice (Oryza sativa L.) crops is in danger of disappearance due to its unsustainable nature. The aim of the present study was to determine the short- and long-term effects of aerobic rice production, combined with conventional and no-tillage practices, on soils' physical, physicochemical, and biological properties, as well as on the rice yield components and productivity in the semi-arid Mediterranean conditions of SW Spain. A field experiment was conducted for three consecutive years (2011, 2012, and 2013), with four treatments: anaerobic with conventional tillage and flooding (CTF), aerobic with conventional tillage and sprinkler irrigation (CTS), aerobic with no-tillage and sprinkler irrigation (NTS), and long-term aerobic with no-tillage and sprinkler irrigation (NTS7). Significant soil properties improvements were achieved after the long-term implementation of no-tillage and sprinkler irrigation (NTS7). The short-term no-tillage and sprinkler irrigated treatment (NTS) gave lower yields than CTF in 2011 and 2012, but reached similar yields in the third year (NTS 8229 kg ha⁻¹; CTF 8926 kg ha⁻¹), with average savings of 75% of the total amount of water applied in CTF. The NTS7 data showed that high yields (reaching 9805 kg ha⁻¹ in 2012) and water savings are sustainable in the long term. The highest water productivity was with NTS7 in 2011 (0.66 g L⁻¹) and 2012 (1.46 g L⁻¹), and with NTS in 2013 (1.05 g L⁻¹). Thus, mid- and long-term implementation of sprinkler irrigation combined with no-tillage may be considered as a potentially productive and sustainable rice cropping system under Mediterranean conditions.     

Energy of biomass sorghum irrigated with reclaimed wastewaters

The sustainability of biomass sorghum (Sorghum bicolor L. Moench) in the Mediterranean environments is linked to the potential to increasing the crop productivity using irrigation water of different qualities: fresh and wastewater. An experiment was conducted in Southern Italy during 2012 and 2013 growing seasons to determine the biomass production and to estimate the yielded energy from sorghum irrigated with fresh water and municipal wastewaters. Two stages of wastewater reclamation process were compared: tertiary and secondary treatments.During the growing seasons, the crop growth (biomass and LAI) was surveyed on sorghum crops irrigated with three water qualities. In order to determine the effects of the irrigation water qualities on the final energy yielded, on the harvested biomass, structural components (cellulose, hemicellulose and lignin contents for deriving the ethanol production) and high heating value were analyzed. The data obtained during two crop seasons showed that, sorghum irrigated with municipal wastewater plant produced more dry biomass (23.3 vs 20.3 t ha⁻¹), energy yield (383 vs 335 GJ ha⁻¹), and ethanol (6824 vs 6092 L ha⁻¹) than sorghum biomass with fresh water. As a consequence, the water efficiency for producing bioenergy increased when the waste waters were supplied in substitution of fresh waters. Different indices were calculated for comparing the effect of the water quality on the water use efficiency (WUE) of biomass sorghum crops.     

Pod and seed numbers as a function of photothermal quotient during the seed set period of field pea (Pisum sativum) crops

The effects of radiation and temperature during the seed set period (SSP) on pod number per square metre (PN m⁻²) and seed number per square metre (SN m⁻²) and those of temperature during grain filling on unit seed weight (USW, milligram per seed) of field pea (Pisum sativum L.) were examined in experiments involving irrigated crops of three or more cultivars of contrasting maturity sown on two or more dates per year from 1996 to 1998 at Buenos Aires, Argentina. The duration of the seed-setting phase was estimated from records of the progress of flowering on the main stem and an estimate (obtained using an optimisation procedure) of the thermal time from flowering at which the uppermost reproductive node reached the final stage of seed abortion (FSSA). The FSSA at a particular node was assumed to be achieved 200 °C day (T_b=4 °C) after flowering at the same node. The grain-filling phase was assumed to run from the achievement of FSSA at the first reproductive node through to 200 °C day (T_b=0 °C) after the date of achievement of the FSSA by the second flowering node.The treatments (cultivar, sowing date, year) produced important ranges of above-ground biomass (AGB) at maturity (271–782 g m⁻²), seed yield (SY, 119–331 g m⁻²), SN (1062–3698 seeds m⁻²) and USW (67–150 mg seed⁻¹). Seed yield was strongly correlated with SN, and there was full compensation between SN and USW in large-seeded cultivars in the high SN range, but not at lower values of SN or in small-seeded cultivars. Both PN (r=0.83) and SN (r=0.87, P<0.0005) were strongly correlated with the mean daily value of the photothermal quotient (PQ=incident radiation/(mean temperature − base temperature)) for the seed-setting phase. Large- and small-seeded cultivars had PN/PQ and SN/PQ relationships with slopes which did not differ among categories but with significantly different intercepts. When the effects of low temperatures during flowering and early grain growth were allowed for, outliers on the PN/PQ and SN/PQ relationships for unstressed crops fell within the confidence limits of the respective linear regressions. Unit seed weight showed a negative response to mean temperature during the grain-filling phase in large- and small-seeded cultivars. We conclude that the relationships established in these experiments, taken together with previous work by other authors, constitute a robust basis for modelling the yield of unstressed field pea crops.     

Energy efficiency of potato production practices for bioethanol feedstock in northern Japan

In 2007–2009, field experiments were conducted to identify agronomic practices affording the lowest energy inputs (i.e. total energy inputs from fuels and other agricultural material inputs required to produce 1 L of ethanol) under potato-based bioethanol feedstock production in northern Japan. On a hectare basis, for a standard 4.4 m⁻² planting density, conventional practices [two inter-row cultivations (weeding and preparation for ridging) and final ridging] yielded an estimate of 4.85 kL ha⁻¹, representing an energy input of 5.86 MJ L⁻¹. The energy input savings arising from the lesser fuel consumption associated with fewer tractor operations under no- and low-ridge cropping practices were outweighed by a reduction in ethanol yields, resulting in slightly greater energy inputs (6.09 ± 0.65 and 5.89 ± 0.30 MJ L⁻¹, respectively). Similarly, poorer ethanol yields outweighed the reduction in energy inputs arising from lessened seed potato production-associated energy inputs under lowered planting densities of 3.8 and 3.3 m⁻², resulting in ethanol yield-based energy inputs of 5.98 ± 0.33 and 6.01 ± 0.41 MJ L⁻¹, respectively. Omitting fungicide applications significantly lowered biocide-related energy inputs, but yielded 20 and 63% lower ethanol yields for Phytophthora-resistant and -susceptible genotypes, respectively, substantially worsening energy efficiencies (6.24 ± 0.42 and 12.2 ± 6.3 MJ L⁻¹). In northern Japan, use of high starch-yielding genotypes served as the only way to increase ethanol yields and improve energy efficiency for potatoes used in bioethanol feedstock production. A 29% greater ethanol yield (6.26 ± 0.46 kL ha⁻¹) and 21% better energy efficiency (4.63 ± 0.23 MJ L⁻¹) were achieved by replacing the standard potato cultivar with a high starch-yielding variety. The yield-based energy inputs with a high starch-yielding potato variety were significantly lower than those with conventional sugar beet in northern Japan (5.82 MJ L⁻¹).     

Effect of treatment with dimethyl dicarbonate on microorganisms and quality of Chinese cabbage

The effects of dimethyl dicarbonate (DMDC) (200 mg L⁻¹) treatment on microorganisms and quality of Chinese cabbage were investigated. The results indicated that in the different tissues treated by DMDC, a significant reduction (P < 0.05) of total aerobic plate count (TAPC) of the leaf (ca. 4.49 log cfu g⁻¹) and stalk (ca. 4.45 log cfu g⁻¹), as well as the count of total yeasts and molds of the leaf (ca. 3.02 log cfu g⁻¹) and stalk (ca. 3.62 log cfu g⁻¹), was obtained in comparison with the control (sterile water dip). However, in the flower bud and/or flower treated by DMDC, the reduction of TAPC (ca. 2.74 log cfu g⁻¹) and counts of total yeasts and molds (ca. 2.26 log cfu g⁻¹) were much lower. DMDC treatment affected appearance and texture early in storage, but an impact on the nutritional composition of Chinese cabbage during storage was not found. Additionally, DMDC treatment significantly (P < 0.05) inhibited the activity of polyphenol oxidase (PPO) and peroxidase (POD) late during storage. Therefore, DMDC is a new and effective alternative for sanitation of Chinese cabbage where a protective measure of appearance quality needs to be adopted.     

White lupine as a beneficial crop in Southern Europe. II. Nitrogen recovery in a legume–oat rotation and a continuous oat–oat

One experiment lasting for two years was carried out at Pegões (central Portugal) to estimate the impact of mature white lupine residue (Lupinus albus L.) on yield of fodder oat (Avena sativa L. cv. Sta. Eulalia) as the next crop in rotation, comparing with the continuous cultivation of cereal, under two tillage practices (conventional tillage and no-till) and fertilized with five mineral nitrogen (N) rates, with three replicates. Oat as a first crop in the rotation provided more N to the agro-ecosystem (63 kg N ha⁻¹) than did lupine (30–59 kg N ha⁻¹). This was at a cost of 100 kg of mineral N ha⁻¹, whereas lupine was grown without addition of N. A positive response of oat as a second crop was obtained per kg of lupine-N added to the system when compared with the continuous oat–oat. The cereal also responded positively to mineral N in the legume amended soil in contrast with the oat–oat sequence where no response was observed, partly due to the fast mineralization rate of lupine residue and a greater soil N immobilization in the continuous oat system. Each kg N ha⁻¹ added to the soil through the application of 73 kg DM ha⁻¹ mature lupine residue (above- and belowground material) increased by 72 kg DM ha⁻¹ the oat biomass produced as the second crop in rotation when 150 kg mineral N ha⁻¹ were split in the season, independent of tillage practice. Mature legume residue conserved in the no-tilled soil depressed the yield of succeeding cereal but less than the continuous oat–oat for both tillage practices, where the application of mineral N did not improve the crop response.     

Water yam (Dioscorea alata L.) growth and yield as affected by the planting date: Experiment and modelling

Tubers of water yam (Dioscorea alata L.) are an important source of carbohydrates for millions of people throughout the tropics. Planting of the crop in the Caribbean and Central America is usually carried out between March and July. As water yam is very sensitive to small variations in photoperiod, changes in the planting date may greatly affect crop development and growth. The aim of this study was to assess the change in the radiation use efficiency (RUE) as a function of the planting date, and its effect on yam growth and yields. For this, we adapted the CropSyst model to simulate yam development and growth. The model was modified, calibrated and tested using two independent data sets from field experiments carried out in Guadeloupe (French Antilles) under non-limiting conditions for water and nutrients and over a wide range of planting dates and photoperiods (e.g. from 12.9 h in March to 11.3 h in October). The planting-harvest period varied from 4.4 months for the late planting dates to 9.1 months for the early planting dates. The RUE was relatively stable in the vegetative phase, increased after tuber initiation (TI) to reach a maximum value during the exponential phase of tuber growth, and then decreased towards the end of growth. The mean value of RUE increased from the early (March, 1.4 g MJ^?1) to the intermediate planting dates (July, 2.7 g MJ^?1), and then decreased for the late planting dates (October, 1.3 g MJ^?1). Maximum RUE was negatively correlated with photoperiod. This particular behaviour of RUE was induced by a source–sink interaction which was controlled by crop development and photoperiod. The model described yam development and growth satisfactorily for all the planting dates: e.g. R² > 0.92 for the correlation between modelled and measured tuber biomass. Yields varied little from the early to the intermediate planting dates because of an offset between the length of the vegetative phase, which determined leaf area, and the level of RUE after TI, which determined the capacity of the crop to fill tubers. Growth and yield drastically decreased for the late planting dates because photoperiod induced fast TI which strongly affected the leaf area. The model was a powerful tool to identify the underlying mechanisms affecting yam yields for some early planting dates, and to assess the factors involved in the high year-to-year variability currently observed in water yam yields.     

Performance of fogged disinfectants to inactivate conidia of Penicillium digitatum within citrus degreening rooms

Fogging with formaldehyde of citrus packinghouses when the fruit are absent is a practice to control conidia of Penicillium digitatum (Pers.) Sacc., the cause of citrus green mold. Replacements for formaldehyde in these facilities are needed because of worker and environmental health issues. To evaluate the effectiveness of candidate sanitizers, craft wood sticks with conidia of P. digitatum were attached throughout commercial citrus ethylene degreening rooms and either water alone or the sanitizers were applied. The rooms were 20 ± 2 °C and humidified to 85–90% relative humidity. Aldehydes, peroxygen compounds, sodium hypochlorite, chlorine dioxide, quaternary ammonium, alcohols, one phenolic compound, and one organic acid were applied with a compressed air assisted atomizer or fan atomizer in a volume of approximately 6 L per 100 m³ of room volume dispensed over a 3 h period. Rates applied were expressed as active ingredient per m³ of room volume. All were compared to formaldehyde applied at 1.98 g m⁻³ of room volume. After 24 h, the craft wood sticks were retrieved, and germination of the conidia assessed. Five sanitizers reduced germination of conidia by more than 95% and equaled formaldehyde in effectiveness. They were (effective rates): (1) glutaraldehyde (0.1 g m⁻³); (2) hydrogen peroxide (4.4 g m⁻³); (3) Citrisol (1.0 g m⁻³), a proprietary mineral oxychloride oxidizer; (4) acetic acid (5.3 g m⁻³); and (5) peracetic acid (2.4 g m⁻³). The toxicity of effective sanitizers was determined by exposure of P. digitatum conidia for 10 min to concentrations of each and the proportion of survivors used to estimate EC₅₀ and EC₉₉ concentrations. The toxicity of the sanitizers in this assay did not predict their effectiveness when applied by fogging, probably because other factors, such as distribution, persistence, droplet size, or vapor pressure also influenced their effectiveness.     

Carbon isotope discrimination and water use efficiency relationships of alfalfa genotypes under irrigated and rain-fed organic farming

Carbon isotope discrimination (Δ) has been proposed as a method for evaluating water use efficiency (WUE) in C₃ plants and as a precise technique for screening plants with higer tolerance under water deficit conditions. In this research, 18 alfalfa genotypes from different geographical origins were evaluated under irrigated and rain-fed conditions in organically managed fields in Austria. Significant differences were found amongst harvests for Δ-shoot under both conditions while genotype by harvest interaction was only significant under irrigated condition. Drought stress under rain-fed condition reduced the overall mean of water use efficiency and carbon isotope discrimination responses(up to 34%), but the ratios of reduction differed for characters and genotypes. Narrow ranges were found for all traits especially for WUE-TBY (total biomass yield) (0.78 kg m⁻³) and Δ-shoot (0.53‰) based on genotype means over locations and years, although variation and ranges were higher under irrigated condition. Regarding the variable and low correlations, simultaneous assessment of genotypes for Δ-shoot and biomass production can ensure the selection of superior genotypes and minimize potential biomass reductions that may result from using Δ-shoot as the only selection criterion to improve WUE. Sitel was the most water use efficient genotype(2.79 and 4.48 kg m⁻³ based on shoot dry matter and total biomass,respectively) across two condition (widely adapted genotype) followed by Mohajeran, Fix232 and Verko under irrigated condition (as specific adapted genotypes) and Vlasta, Sanditi, Ghara-aghaj under rain-fed condition.     

Rheological and chemical predictors of texture and taste in dessert banana (Musa spp.)

To be able to account for sensory qualities earlier in the assessment of a new banana hybrid in a selection scheme, predicting the sensory perception of banana texture and taste by instrumental parameters was investigated. Thirteen cultivated banana and four new triploid hybrids were characterized by sensory profiling, and rheological and chemical analyses. Multilinear regressions were used to calibrate predictions using 13 cultivated bananas, and the quality of predictions was validated using four hybrids. The sensory characteristics sourness and sweetness were predicted by titratable acidity (R² = 0.68) and pH (R² = 0.66). Malate and citrate were the main contributors to sweetness and sourness. Astringency was predicted by total tannins (R² = 0.55). Rheological parameters from texture profile analyses (stress at fracture, fracturability) were more suitable than pulp puncture force to predict the sensory texture properties firmness (R² = 0.47) and melting (R² = 0.60). These textural properties were predicted by titratable acidity and dry matter content (R² = 0.62). Predictions of mealiness, adhesiveness, and heterogeneity were not efficient. Differences of 3.6–3.7 meq 100 g⁻¹ FW in titratable acidity or of 0.30 g 100 g⁻¹ FW in malate or citrate were required to ensure a detectable difference in sourness or sweetness (p = 0.9). Pulp puncture force needed to differ by a minimum of 0.9 N before a difference in firmness could be perceived by the panelists. In conclusion, while models to predict sourness and sweetness can now be used for high throughput phenotyping, we recommend additional tests for other sensory attributes.     

Biomass production and N balance of giant reed (Arundo donax L.) under high water and N input in Mediterranean environments

This study aimed to evaluate the productivity of Arundo donax under good water and N availability coming from non-conventional sources, in different Italian environments (Padova and Bologna in the north, Reggio Calabria and Catania in the south) in relation to three harvest periods (autumn; mid-winter; late-winter).In the northern locations A. donax had already reached maximum productivity the year after transplanting, with 85 and 98 t ha⁻¹ of dry matter at Padova and Bologna, respectively. At Reggio Calabria and Catania a further biomass increase was obtained from the second to third year of cultivation, when production was 62 t ha⁻¹ and 51 t ha⁻¹, respectively.The average dry matter production was 74, 66 and 65 t ha⁻¹ with autumn, mid-winter and late-winter harvesting, respectively.Under N input ranging from 225 to 329 kg ha⁻¹ year⁻¹ at the different locations, the apparent N balance (input–output) was negative except in Catania indicating a great potential of A. donax to provide high N uptake, which would be a useful feature in environments under the European Nitrates Directive.     

Treatment with chlorine dioxide extends the vase life of selected cut flowers

The accumulation of bacteria in vase water is often associated with premature senescence in many cut flower species. In the present study, we tested the efficacy of aqueous chlorine dioxide (ClO₂) to extend flower display life by preventing the build-up of bacteria in vase solutions. The addition of 2 or 10 μL L⁻¹ ClO₂ to clean deionized water extended the vase life of Alstroemeria peruviana ‘Senna’, Antirrhinum majus ‘Potomic Pink’, Dianthus caryophyllus ‘Pasha’, Gerbera jamesonii ‘Monarch’, Gypsophila paniculata ‘Crystal’ and ‘Perfecta’, Lilium asiaticum ‘Vermeer’, Matthiola incana ‘Ruby Red’ and Rosa hybrida ‘Charlotte’ flowers by 0.9–13.4 d (7–77%) relative to control (i.e. 0 μL L⁻¹ ClO₂) stems. The beneficial effects of ClO₂ treatment were associated with a reduction in the accumulation of aerobic bacteria in vase water and on cut surfaces of flower stems. ClO₂ treatment was also effective in maintaining or extending the vase life of A. majus ‘Potomic Pink’, Dendrathema × grandiflorum ‘Albatron’, G. paniculata ‘Perfecta’ and M. incana ‘Ruby Red’ flowers even when stems were placed into water containing 10¹¹ CFU L⁻¹ bacteria. The efficacy of 10 μL L⁻¹ ClO₂ in vase water containing 0.2 g L⁻¹ citric acid and 10 g L⁻¹ sucrose to extend the display life of G. jamesonii ‘Lorca’ and ‘Vilassar’ flowers was equal to or greater than other tested biocides (i.e. aluminum sulfate, dichloroisocyanuric acid, 8-hydroxyquinoline sulfate, Physan 20™, sodium hypochlorite). Taken collectively, the results of the present study highlight the potential of aqueous ClO₂ for use as an alternative antibacterial agent in flower vase solutions.